TY  - JOUR
AU  - Saičić, Zorica
AU  - Radojičić, Ratko M.
AU  - Spasić, Snežana D.
AU  - Jovanović, Tamara B.
AU  - Simić-Krstić, Jovana B.
PY  - 2004
PY  - 2004
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/549
AB  - The C3 clone of B-16 mouse melanoma was cultured for 1, 6 and 9 days and analyzed. The changes which are not directly linked to melanogenesis in the B-16 / C3 cultures during their maturation were characterized. Early (1 day) confluent (6 days) and old (9 days) cell cultures are distinguished by their leucine aminopeptidase (LAP) and α-naphthyl acetate esterase (ANAE) isoenzyme patterns. Both quantitative and qualitative changes in LAP and ANAE isoenzyme can be observed during culture maturation. There is an increase in the activity of the enzyme copper, zinc-containing superoxide-dismutase (CuZn SOD). The increaase in the CuZn SOD enzyme activity might be related to B-16/C3 cell melanogenesis and / or to differentiation.
AB  - C3 klonovi B-16 melanoma miša su gajeni u kulturi 1, 6 i 9 dana i potom analizirani. Izvršena je karakterizacija promena B-16/C3 ćelijskih kultura tokom njihove maturacije koje nisu direktno povezane sa melanogenezom. Pokazano je da se kulture u početnoj fazi (1 dan), konfluentnoj fazi (6 dana) i kasnoj fazi (9 dana) ćelijskog rasta razlikuju na osnovu izoenzimskih spektara leucin-aminopeptidaze i alfa-naftil-acetat-esteraze. Tokom razvitka ćelijskih kultura B-16/C3 zapažene su kvantitativne i kvalitativne promene leucin-aminopeptidaze i alfa-naftil-acetat-esteraze u njihovim izoenzimskim spektrima. Utvrđeno je da postoji porast aktivnosti bakar-cink superoksid-dismutaze tokom starenja kulture. Porast aktivnosti bakar-cink superoksid-dismutaze može biti povezan sa melanogenezom i/ili diferencijacijom B-16/C3 ćelija.
T2  - Journal of the Serbian Chemical Society
T1  - Aktivnost superoksid-dismutaze tokom maturacije kulture ćelija b-16 melanoma miša
T1  - Superoxide dismutase activity as a function of culture aging of B-16 mouse melanoma cells
IS  - 12
VL  - 69
SP  - 1005
EP  - 1011
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_549
ER  - 

@article{
author = "Saičić, Zorica and Radojičić, Ratko M. and Spasić, Snežana D. and Jovanović, Tamara B. and Simić-Krstić, Jovana B.",
year = "2004, 2004",
abstract = "The C3 clone of B-16 mouse melanoma was cultured for 1, 6 and 9 days and analyzed. The changes which are not directly linked to melanogenesis in the B-16 / C3 cultures during their maturation were characterized. Early (1 day) confluent (6 days) and old (9 days) cell cultures are distinguished by their leucine aminopeptidase (LAP) and α-naphthyl acetate esterase (ANAE) isoenzyme patterns. Both quantitative and qualitative changes in LAP and ANAE isoenzyme can be observed during culture maturation. There is an increase in the activity of the enzyme copper, zinc-containing superoxide-dismutase (CuZn SOD). The increaase in the CuZn SOD enzyme activity might be related to B-16/C3 cell melanogenesis and / or to differentiation., C3 klonovi B-16 melanoma miša su gajeni u kulturi 1, 6 i 9 dana i potom analizirani. Izvršena je karakterizacija promena B-16/C3 ćelijskih kultura tokom njihove maturacije koje nisu direktno povezane sa melanogenezom. Pokazano je da se kulture u početnoj fazi (1 dan), konfluentnoj fazi (6 dana) i kasnoj fazi (9 dana) ćelijskog rasta razlikuju na osnovu izoenzimskih spektara leucin-aminopeptidaze i alfa-naftil-acetat-esteraze. Tokom razvitka ćelijskih kultura B-16/C3 zapažene su kvantitativne i kvalitativne promene leucin-aminopeptidaze i alfa-naftil-acetat-esteraze u njihovim izoenzimskim spektrima. Utvrđeno je da postoji porast aktivnosti bakar-cink superoksid-dismutaze tokom starenja kulture. Porast aktivnosti bakar-cink superoksid-dismutaze može biti povezan sa melanogenezom i/ili diferencijacijom B-16/C3 ćelija.",
journal = "Journal of the Serbian Chemical Society",
title = "Aktivnost superoksid-dismutaze tokom maturacije kulture ćelija b-16 melanoma miša, Superoxide dismutase activity as a function of culture aging of B-16 mouse melanoma cells",
number = "12",
volume = "69",
pages = "1005-1011",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_549"
}

Saičić, Z., Radojičić, R. M., Spasić, S. D., Jovanović, T. B.,& Simić-Krstić, J. B.. (2004). Aktivnost superoksid-dismutaze tokom maturacije kulture ćelija b-16 melanoma miša. in Journal of the Serbian Chemical Society, 69(12), 1005-1011.
https://hdl.handle.net/21.15107/rcub_ibiss_549

Saičić Z, Radojičić RM, Spasić SD, Jovanović TB, Simić-Krstić JB. Aktivnost superoksid-dismutaze tokom maturacije kulture ćelija b-16 melanoma miša. in Journal of the Serbian Chemical Society. 2004;69(12):1005-1011.
https://hdl.handle.net/21.15107/rcub_ibiss_549 .

Saičić, Zorica, Radojičić, Ratko M., Spasić, Snežana D., Jovanović, Tamara B., Simić-Krstić, Jovana B., "Aktivnost superoksid-dismutaze tokom maturacije kulture ćelija b-16 melanoma miša" in Journal of the Serbian Chemical Society, 69, no. 12 (2004):1005-1011,
https://hdl.handle.net/21.15107/rcub_ibiss_549 .

TY  - JOUR
AU  - Radojičić, Ratko M.
AU  - Spasić, Mihajlo
AU  - Saičić, Zorica
AU  - Simić-Krstić, Jovana B.
PY  - 2004
PY  - 2004
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/111
AB  - B-I6/C3 mouse melanoma cells undergo melanogenesis and differentiation 9 days after plating under usual conditions. In our experiments the effect of exogenous bovine CuZn superoxide dismutase (CuZn SOD) on B-I6/C3 cells in cultures was studied. The exogenous CuZn SOD was added, 24 hours after cell plating, in growth medium, which either contain or not Fetal Calf Serum (FCS). B-I6/C3 melanoma cells growth was followed over 5 days. Different effect of CuZn SOD on the culture was observed in relation to FCS present in growth medium. CuZn SOD induced a dose dependent increase in melanin content of B-I6/C3 cells in serum deprived medium. Our results are discussed in respect to dismutating CuZn SOD activity, which may act to an enhanced level of oxidative stress, related to the higher metabolism in melanoma cells. On the other hand we supposed that CuZn SOD produced an elevated level of hydrogen peroxide. Therefore hydrogen peroxide than may play some role in cell differentiation as transmembrane messenger.
AB  - Ćelije B-I6/C3 melanoma miša melaniziraju i diferenciraju devetog dana posle zasađivanja pod uobičajenim uslovima. U našim eksperimentima smo proučavali uticaj egzogeno dodavane CuZn superoksid-dismutaze ( CuZn SOD) na ćelije B-I6/C3 u kulturi. Egzogena CuZn SOD je dodavana 24 časa posle zasađivanja ćelija u medijume za rast koji su sadržavali ili ne goveđi fetalni serum. Rast ćelija B-I6/C3 je praćen pet dana. Zapažen je različit uticaj CuZn SOD na ćelijske kulture gajene sa serumom ili bez njega. CuZn SOD izaziva dozno zavisnu sintezu i nakupljanje melanina u ćelijama koje su rasle u medijumu koji nije sadržavao goveđi fetalni serum. Naši rezultatu se mogu diskutovati u svetlu dismutacione aktivnosti CuZn SOD koja deluje na povećani nivo oksidacionog stresa izazvanog visokim metabolizmom melanoma ćelija. S druge strane, pretpostavili smo da CuZn SOD svojom aktivnošću proizvodi više vodonik peroksida. Vodonik peroksid, nadalje, može da ima izvesnu ulogu u diferencijaciji ćelija kao transmembranski signalni molekul.
T2  - Acta biologica iugoslavica - serija C: Physiologica et pharmacologica acta
T1  - Uticaj goveđe CuZn superoksid dismutaze na kulture ćelija Ci klona B-16 mišijeg melanoma
T1  - Effect of bovine CuZn superoxide dismutase on C3 clone of B-16 mouse melanome cells in culture
IS  - 1-3
VL  - 40
SP  - 103
EP  - 108
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_111
ER  - 

@article{
author = "Radojičić, Ratko M. and Spasić, Mihajlo and Saičić, Zorica and Simić-Krstić, Jovana B.",
year = "2004, 2004",
abstract = "B-I6/C3 mouse melanoma cells undergo melanogenesis and differentiation 9 days after plating under usual conditions. In our experiments the effect of exogenous bovine CuZn superoxide dismutase (CuZn SOD) on B-I6/C3 cells in cultures was studied. The exogenous CuZn SOD was added, 24 hours after cell plating, in growth medium, which either contain or not Fetal Calf Serum (FCS). B-I6/C3 melanoma cells growth was followed over 5 days. Different effect of CuZn SOD on the culture was observed in relation to FCS present in growth medium. CuZn SOD induced a dose dependent increase in melanin content of B-I6/C3 cells in serum deprived medium. Our results are discussed in respect to dismutating CuZn SOD activity, which may act to an enhanced level of oxidative stress, related to the higher metabolism in melanoma cells. On the other hand we supposed that CuZn SOD produced an elevated level of hydrogen peroxide. Therefore hydrogen peroxide than may play some role in cell differentiation as transmembrane messenger., Ćelije B-I6/C3 melanoma miša melaniziraju i diferenciraju devetog dana posle zasađivanja pod uobičajenim uslovima. U našim eksperimentima smo proučavali uticaj egzogeno dodavane CuZn superoksid-dismutaze ( CuZn SOD) na ćelije B-I6/C3 u kulturi. Egzogena CuZn SOD je dodavana 24 časa posle zasađivanja ćelija u medijume za rast koji su sadržavali ili ne goveđi fetalni serum. Rast ćelija B-I6/C3 je praćen pet dana. Zapažen je različit uticaj CuZn SOD na ćelijske kulture gajene sa serumom ili bez njega. CuZn SOD izaziva dozno zavisnu sintezu i nakupljanje melanina u ćelijama koje su rasle u medijumu koji nije sadržavao goveđi fetalni serum. Naši rezultatu se mogu diskutovati u svetlu dismutacione aktivnosti CuZn SOD koja deluje na povećani nivo oksidacionog stresa izazvanog visokim metabolizmom melanoma ćelija. S druge strane, pretpostavili smo da CuZn SOD svojom aktivnošću proizvodi više vodonik peroksida. Vodonik peroksid, nadalje, može da ima izvesnu ulogu u diferencijaciji ćelija kao transmembranski signalni molekul.",
journal = "Acta biologica iugoslavica - serija C: Physiologica et pharmacologica acta",
title = "Uticaj goveđe CuZn superoksid dismutaze na kulture ćelija Ci klona B-16 mišijeg melanoma, Effect of bovine CuZn superoxide dismutase on C3 clone of B-16 mouse melanome cells in culture",
number = "1-3",
volume = "40",
pages = "103-108",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_111"
}

Radojičić, R. M., Spasić, M., Saičić, Z.,& Simić-Krstić, J. B.. (2004). Uticaj goveđe CuZn superoksid dismutaze na kulture ćelija Ci klona B-16 mišijeg melanoma. in Acta biologica iugoslavica - serija C: Physiologica et pharmacologica acta, 40(1-3), 103-108.
https://hdl.handle.net/21.15107/rcub_ibiss_111

Radojičić RM, Spasić M, Saičić Z, Simić-Krstić JB. Uticaj goveđe CuZn superoksid dismutaze na kulture ćelija Ci klona B-16 mišijeg melanoma. in Acta biologica iugoslavica - serija C: Physiologica et pharmacologica acta. 2004;40(1-3):103-108.
https://hdl.handle.net/21.15107/rcub_ibiss_111 .

Radojičić, Ratko M., Spasić, Mihajlo, Saičić, Zorica, Simić-Krstić, Jovana B., "Uticaj goveđe CuZn superoksid dismutaze na kulture ćelija Ci klona B-16 mišijeg melanoma" in Acta biologica iugoslavica - serija C: Physiologica et pharmacologica acta, 40, no. 1-3 (2004):103-108,
https://hdl.handle.net/21.15107/rcub_ibiss_111 .