TY  - JOUR
AU  - Banković, Jasna Z.
AU  - Joerg, Andrae
AU  - Todorović, Nataša
AU  - Podolski-Renić, Ana
AU  - Milošević, Zorica Z.
AU  - Miljković, Đorđe
AU  - Krause, Jannike
AU  - Ruždijić, Sabera
AU  - Tanić, Nikola
AU  - Pešić, Milica
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1018
AB  - Most chemotherapeutics harm normal cells causing severe side effects and induce the development of resistance in cancer cells. Antimicrobial peptides (AMPs), recognized as anticancer agents, may overcome these limitations. The most studied mechanism underlying multidrug resistance (MDR) is the over-expression of cell membrane transporter P-glycoprotein (P-gp), which extrudes a variety of hydrophobic drugs. Additionally, P-gp contributes to cell membrane composition and increases the net negative charge on cell surface. We postulated that NK-lysin derived cationic peptide NK-2 might discriminate and preferentially eliminate P-gp over-expressing cancer cells. To test this hypothesis, we employed MDR non-small cell lung carcinoma (NCI-H460/R) and colorectal carcinoma (DLD1-TxR) cell lines with high P-gp expression. MDR cancer cells that survived NK-2 treatment had decreased P-gp expression and were more susceptible to doxorubicin. We found that NK-2 more readily eliminated P-gp high-expressing cells. Acting in 'carpet-like' manner NK-2 co-localized with P-gp on the MDR cancer cell membrane. The inhibition of P-gp reduced the NK-2 effect in MDR cancer cells and, vice versa, NK-2 decreased P-gp transport activity. In conclusion, NK-2 could modulate MDR in unique way, eliminating the P-gp high-expressing cells from heterogeneous cancers and making them more vulnerable to classical drug treatment. (C) 2012 Elsevier Inc. All rights reserved.
PB  - Elsevier
T2  - Experimental Cell Research
T1  - The elimination of P-glycoprotein over-expressing cancer cells by antimicrobial cationic peptide NK-2: The unique way of multi-drug resistance modulation
IS  - 7
VL  - 319
DO  - 10.1016/j.yexcr.2012.12.017
SP  - 1013
EP  - 1027
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1018
ER  - 

@article{
author = "Banković, Jasna Z. and Joerg, Andrae and Todorović, Nataša and Podolski-Renić, Ana and Milošević, Zorica Z. and Miljković, Đorđe and Krause, Jannike and Ruždijić, Sabera and Tanić, Nikola and Pešić, Milica",
year = "2013",
abstract = "Most chemotherapeutics harm normal cells causing severe side effects and induce the development of resistance in cancer cells. Antimicrobial peptides (AMPs), recognized as anticancer agents, may overcome these limitations. The most studied mechanism underlying multidrug resistance (MDR) is the over-expression of cell membrane transporter P-glycoprotein (P-gp), which extrudes a variety of hydrophobic drugs. Additionally, P-gp contributes to cell membrane composition and increases the net negative charge on cell surface. We postulated that NK-lysin derived cationic peptide NK-2 might discriminate and preferentially eliminate P-gp over-expressing cancer cells. To test this hypothesis, we employed MDR non-small cell lung carcinoma (NCI-H460/R) and colorectal carcinoma (DLD1-TxR) cell lines with high P-gp expression. MDR cancer cells that survived NK-2 treatment had decreased P-gp expression and were more susceptible to doxorubicin. We found that NK-2 more readily eliminated P-gp high-expressing cells. Acting in 'carpet-like' manner NK-2 co-localized with P-gp on the MDR cancer cell membrane. The inhibition of P-gp reduced the NK-2 effect in MDR cancer cells and, vice versa, NK-2 decreased P-gp transport activity. In conclusion, NK-2 could modulate MDR in unique way, eliminating the P-gp high-expressing cells from heterogeneous cancers and making them more vulnerable to classical drug treatment. (C) 2012 Elsevier Inc. All rights reserved.",
publisher = "Elsevier",
journal = "Experimental Cell Research",
title = "The elimination of P-glycoprotein over-expressing cancer cells by antimicrobial cationic peptide NK-2: The unique way of multi-drug resistance modulation",
number = "7",
volume = "319",
doi = "10.1016/j.yexcr.2012.12.017",
pages = "1013-1027",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1018"
}

Banković, J. Z., Joerg, A., Todorović, N., Podolski-Renić, A., Milošević, Z. Z., Miljković, Đ., Krause, J., Ruždijić, S., Tanić, N.,& Pešić, M.. (2013). The elimination of P-glycoprotein over-expressing cancer cells by antimicrobial cationic peptide NK-2: The unique way of multi-drug resistance modulation. in Experimental Cell Research
Elsevier., 319(7), 1013-1027.
https://doi.org/10.1016/j.yexcr.2012.12.017
https://hdl.handle.net/21.15107/rcub_ibiss_1018

Banković JZ, Joerg A, Todorović N, Podolski-Renić A, Milošević ZZ, Miljković Đ, Krause J, Ruždijić S, Tanić N, Pešić M. The elimination of P-glycoprotein over-expressing cancer cells by antimicrobial cationic peptide NK-2: The unique way of multi-drug resistance modulation. in Experimental Cell Research. 2013;319(7):1013-1027.
doi:10.1016/j.yexcr.2012.12.017
https://hdl.handle.net/21.15107/rcub_ibiss_1018 .

Banković, Jasna Z., Joerg, Andrae, Todorović, Nataša, Podolski-Renić, Ana, Milošević, Zorica Z., Miljković, Đorđe, Krause, Jannike, Ruždijić, Sabera, Tanić, Nikola, Pešić, Milica, "The elimination of P-glycoprotein over-expressing cancer cells by antimicrobial cationic peptide NK-2: The unique way of multi-drug resistance modulation" in Experimental Cell Research, 319, no. 7 (2013):1013-1027,
https://doi.org/10.1016/j.yexcr.2012.12.017 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1018 .

TY  - CONF
AU  - Ruždijić, Sabera
AU  - Banković, Jasna Z.
AU  - Podolski-Renić, Ana
AU  - Pešić, Milica
AU  - Milošević, Zorica Z.
AU  - Tanić, Nikola T
AU  - Andra, J
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1161
C3  - European Journal of Cancer
T1  - NK-lysin Derived Peptide (NK-2) Sensitizes Resistant Cancer Cells to Classic Chemotherapeutics by Selective Killing of P-glycoprotein Over-expressing Cells
IS  - null
VL  - 48
EP  - S225
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1161
ER  - 

@conference{
author = "Ruždijić, Sabera and Banković, Jasna Z. and Podolski-Renić, Ana and Pešić, Milica and Milošević, Zorica Z. and Tanić, Nikola T and Andra, J",
year = "2012",
journal = "European Journal of Cancer",
title = "NK-lysin Derived Peptide (NK-2) Sensitizes Resistant Cancer Cells to Classic Chemotherapeutics by Selective Killing of P-glycoprotein Over-expressing Cells",
number = "null",
volume = "48",
pages = "S225",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1161"
}

Ruždijić, S., Banković, J. Z., Podolski-Renić, A., Pešić, M., Milošević, Z. Z., Tanić, N. T.,& Andra, J.. (2012). NK-lysin Derived Peptide (NK-2) Sensitizes Resistant Cancer Cells to Classic Chemotherapeutics by Selective Killing of P-glycoprotein Over-expressing Cells. in European Journal of Cancer, 48(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1161

Ruždijić S, Banković JZ, Podolski-Renić A, Pešić M, Milošević ZZ, Tanić NT, Andra J. NK-lysin Derived Peptide (NK-2) Sensitizes Resistant Cancer Cells to Classic Chemotherapeutics by Selective Killing of P-glycoprotein Over-expressing Cells. in European Journal of Cancer. 2012;48(null):null-S225.
https://hdl.handle.net/21.15107/rcub_ibiss_1161 .

Ruždijić, Sabera, Banković, Jasna Z., Podolski-Renić, Ana, Pešić, Milica, Milošević, Zorica Z., Tanić, Nikola T, Andra, J, "NK-lysin Derived Peptide (NK-2) Sensitizes Resistant Cancer Cells to Classic Chemotherapeutics by Selective Killing of P-glycoprotein Over-expressing Cells" in European Journal of Cancer, 48, no. null (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1161 .

TY  - CONF
AU  - Podolski-Renić, Ana
AU  - Pešić, Milica
AU  - Chiourea, M
AU  - Banković, Jasna Z.
AU  - Anđelković, Tijana
AU  - Milošević, Zorica Z.
AU  - Milinković, Vedrana P.
AU  - Gagos, S
AU  - Tanić, Nikola T
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1162
C3  - European Journal of Cancer
T1  - Characterization of Newly Established P-glycoprotein Over-expressing Multi-drug Resistant Human Cancer Cell Lines
IS  - null
VL  - 48
EP  - S244
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1162
ER  - 

@conference{
author = "Podolski-Renić, Ana and Pešić, Milica and Chiourea, M and Banković, Jasna Z. and Anđelković, Tijana and Milošević, Zorica Z. and Milinković, Vedrana P. and Gagos, S and Tanić, Nikola T",
year = "2012",
journal = "European Journal of Cancer",
title = "Characterization of Newly Established P-glycoprotein Over-expressing Multi-drug Resistant Human Cancer Cell Lines",
number = "null",
volume = "48",
pages = "S244",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1162"
}

Podolski-Renić, A., Pešić, M., Chiourea, M., Banković, J. Z., Anđelković, T., Milošević, Z. Z., Milinković, V. P., Gagos, S.,& Tanić, N. T.. (2012). Characterization of Newly Established P-glycoprotein Over-expressing Multi-drug Resistant Human Cancer Cell Lines. in European Journal of Cancer, 48(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1162

Podolski-Renić A, Pešić M, Chiourea M, Banković JZ, Anđelković T, Milošević ZZ, Milinković VP, Gagos S, Tanić NT. Characterization of Newly Established P-glycoprotein Over-expressing Multi-drug Resistant Human Cancer Cell Lines. in European Journal of Cancer. 2012;48(null):null-S244.
https://hdl.handle.net/21.15107/rcub_ibiss_1162 .

Podolski-Renić, Ana, Pešić, Milica, Chiourea, M, Banković, Jasna Z., Anđelković, Tijana, Milošević, Zorica Z., Milinković, Vedrana P., Gagos, S, Tanić, Nikola T, "Characterization of Newly Established P-glycoprotein Over-expressing Multi-drug Resistant Human Cancer Cell Lines" in European Journal of Cancer, 48, no. null (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1162 .

TY  - JOUR
AU  - Milinković, Vedrana P.
AU  - Banković, Jasna Z.
AU  - Rakić, Miodrag L
AU  - Milosević, Nebojsa T
AU  - Stanković, Tijana
AU  - Joković, Milos B
AU  - Milošević, Zorica Z.
AU  - Skender-Gazibara, Milica K
AU  - Podolski-Renić, Ana
AU  - Pešić, Milica
AU  - Ruždijić, Sabera
AU  - Tanić, Nikola T
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1108
AB  - The purpose of this study was to detect the level of genomic instability and p53 alterations in anaplastic astrocytoma and primary glioblastoma patients, and to evaluate their impact on glioma pathogenesis and patients outcome. AP-PCR DNA profiling revealed two types of genetic differences between tumor and normal tissue: qualitative changes which represent accumulation of changes in DNA sequence and are the manifestation of microsatellite and point mutation instability (MIN-PIN) and quantitative changes which represent amplifications or deletions of existing chromosomal material and are the manifestation of chromosomal instability (CIN). Both types of alterations were present in all analyzed samples contributing almost equally to the total level of genomic instability, and showing no differences between histological subtypes. p53 alterations were detected in 40% of samples, predominantly in anaplastic astrocytoma. The higher level of genomic instability was observed in elderly patients (>50 years) and patents with primary glioblastoma. Level of genomic instability had no impact on patients' survival, while presence of p53 alterations seemed to be a favorable prognostic factor in this case. Our results indicate that extensive genomic instability is one of the main features of malignant gliomas. (C) 2012 Elsevier Inc. All rights reserved.
T2  - Experimental and Molecular Pathology
T1  - Genomic instability and p53 alterations in patients with malignant glioma
IS  - 2
VL  - 93
SP  - 67
EP  - 206
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1108
ER  - 

@article{
author = "Milinković, Vedrana P. and Banković, Jasna Z. and Rakić, Miodrag L and Milosević, Nebojsa T and Stanković, Tijana and Joković, Milos B and Milošević, Zorica Z. and Skender-Gazibara, Milica K and Podolski-Renić, Ana and Pešić, Milica and Ruždijić, Sabera and Tanić, Nikola T",
year = "2012",
abstract = "The purpose of this study was to detect the level of genomic instability and p53 alterations in anaplastic astrocytoma and primary glioblastoma patients, and to evaluate their impact on glioma pathogenesis and patients outcome. AP-PCR DNA profiling revealed two types of genetic differences between tumor and normal tissue: qualitative changes which represent accumulation of changes in DNA sequence and are the manifestation of microsatellite and point mutation instability (MIN-PIN) and quantitative changes which represent amplifications or deletions of existing chromosomal material and are the manifestation of chromosomal instability (CIN). Both types of alterations were present in all analyzed samples contributing almost equally to the total level of genomic instability, and showing no differences between histological subtypes. p53 alterations were detected in 40% of samples, predominantly in anaplastic astrocytoma. The higher level of genomic instability was observed in elderly patients (>50 years) and patents with primary glioblastoma. Level of genomic instability had no impact on patients' survival, while presence of p53 alterations seemed to be a favorable prognostic factor in this case. Our results indicate that extensive genomic instability is one of the main features of malignant gliomas. (C) 2012 Elsevier Inc. All rights reserved.",
journal = "Experimental and Molecular Pathology",
title = "Genomic instability and p53 alterations in patients with malignant glioma",
number = "2",
volume = "93",
pages = "67-206",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1108"
}

Milinković, V. P., Banković, J. Z., Rakić, M. L., Milosević, N. T., Stanković, T., Joković, M. B., Milošević, Z. Z., Skender-Gazibara, M. K., Podolski-Renić, A., Pešić, M., Ruždijić, S.,& Tanić, N. T.. (2012). Genomic instability and p53 alterations in patients with malignant glioma. in Experimental and Molecular Pathology, 93(2), 67-206.
https://hdl.handle.net/21.15107/rcub_ibiss_1108

Milinković VP, Banković JZ, Rakić ML, Milosević NT, Stanković T, Joković MB, Milošević ZZ, Skender-Gazibara MK, Podolski-Renić A, Pešić M, Ruždijić S, Tanić NT. Genomic instability and p53 alterations in patients with malignant glioma. in Experimental and Molecular Pathology. 2012;93(2):67-206.
https://hdl.handle.net/21.15107/rcub_ibiss_1108 .

Milinković, Vedrana P., Banković, Jasna Z., Rakić, Miodrag L, Milosević, Nebojsa T, Stanković, Tijana, Joković, Milos B, Milošević, Zorica Z., Skender-Gazibara, Milica K, Podolski-Renić, Ana, Pešić, Milica, Ruždijić, Sabera, Tanić, Nikola T, "Genomic instability and p53 alterations in patients with malignant glioma" in Experimental and Molecular Pathology, 93, no. 2 (2012):67-206,
https://hdl.handle.net/21.15107/rcub_ibiss_1108 .