TY  - JOUR
AU  - Nestorović Živković, Jasmina
AU  - Aničić, Neda
AU  - Matekalo, Dragana
AU  - Skorić, Marijana
AU  - Filipović, Biljana
AU  - Marković, Tijana
AU  - Dmitrović, Slavica
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6561
AB  - Plants have developed a variety of defense mechanisms that allow them to recognize
and respond to specific stressors by triggering complex signaling networks that cause appropriate
biochemical changes to overcome the stress. In this study, we subjected in vitro grown N. nervosa
plants to PEG-induced dehydration stress for 1 day (1DPT), 3 days (3DPT) and 6 days (6DPT). Our
study investigated antioxidant enzyme activities, including catalase (CAT), peroxidase (POX) and
superoxide dismutase (SOD), unveiling dynamic responses to PEG-induced water stress. CAT levels
increased initially (1DPT) but declined with prolonged treatment; while POX activity significantly
increased at 3DPT and 6DPT; and SOD, particularly the Mn-SOD3 isoform, demonstrated a substantial
increase, emphasizing its role in the enzymatic free-radical scavenging activity. Furthermore,
examination of the phenolic acid content revealed that rosmarinic acid (RA) was the predominant
phenolic compound, followed by chlorogenic acid (CHLA), while ferulic acid (FA) and caffeic acid
(CAFFA) were present in lower concentrations. Notably, PEG-induced dehydration significantly
boosted RA content in N. nervosa plants at 3DPT. This increase highlights the plant’s response to
oxidative stress conditions and its role in non-enzymatic antioxidant defense mechanisms. These
findings significantly contribute to our comprehension of N. nervosa’s adaptive strategies under PEG induced dehydration stress, offering valuable insights into plant stress physiology within industrial
and agricultural contexts.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Polyethylene glycol (PEG)-induced dehydration alters enzymatic and non-enzymatic components of the antioxidant defense system in Nepeta nervosa Royle ex Bentham.
IS  - 12
VL  - 9
DO  - 10.3390/horticulturae9121277
SP  - 1277
ER  - 

@article{
author = "Nestorović Živković, Jasmina and Aničić, Neda and Matekalo, Dragana and Skorić, Marijana and Filipović, Biljana and Marković, Tijana and Dmitrović, Slavica",
year = "2023",
abstract = "Plants have developed a variety of defense mechanisms that allow them to recognize
and respond to specific stressors by triggering complex signaling networks that cause appropriate
biochemical changes to overcome the stress. In this study, we subjected in vitro grown N. nervosa
plants to PEG-induced dehydration stress for 1 day (1DPT), 3 days (3DPT) and 6 days (6DPT). Our
study investigated antioxidant enzyme activities, including catalase (CAT), peroxidase (POX) and
superoxide dismutase (SOD), unveiling dynamic responses to PEG-induced water stress. CAT levels
increased initially (1DPT) but declined with prolonged treatment; while POX activity significantly
increased at 3DPT and 6DPT; and SOD, particularly the Mn-SOD3 isoform, demonstrated a substantial
increase, emphasizing its role in the enzymatic free-radical scavenging activity. Furthermore,
examination of the phenolic acid content revealed that rosmarinic acid (RA) was the predominant
phenolic compound, followed by chlorogenic acid (CHLA), while ferulic acid (FA) and caffeic acid
(CAFFA) were present in lower concentrations. Notably, PEG-induced dehydration significantly
boosted RA content in N. nervosa plants at 3DPT. This increase highlights the plant’s response to
oxidative stress conditions and its role in non-enzymatic antioxidant defense mechanisms. These
findings significantly contribute to our comprehension of N. nervosa’s adaptive strategies under PEG induced dehydration stress, offering valuable insights into plant stress physiology within industrial
and agricultural contexts.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Polyethylene glycol (PEG)-induced dehydration alters enzymatic and non-enzymatic components of the antioxidant defense system in Nepeta nervosa Royle ex Bentham.",
number = "12",
volume = "9",
doi = "10.3390/horticulturae9121277",
pages = "1277"
}

Nestorović Živković, J., Aničić, N., Matekalo, D., Skorić, M., Filipović, B., Marković, T.,& Dmitrović, S.. (2023). Polyethylene glycol (PEG)-induced dehydration alters enzymatic and non-enzymatic components of the antioxidant defense system in Nepeta nervosa Royle ex Bentham.. in Horticulturae
Basel: MDPI., 9(12), 1277.
https://doi.org/10.3390/horticulturae9121277

Nestorović Živković J, Aničić N, Matekalo D, Skorić M, Filipović B, Marković T, Dmitrović S. Polyethylene glycol (PEG)-induced dehydration alters enzymatic and non-enzymatic components of the antioxidant defense system in Nepeta nervosa Royle ex Bentham.. in Horticulturae. 2023;9(12):1277.
doi:10.3390/horticulturae9121277 .

Nestorović Živković, Jasmina, Aničić, Neda, Matekalo, Dragana, Skorić, Marijana, Filipović, Biljana, Marković, Tijana, Dmitrović, Slavica, "Polyethylene glycol (PEG)-induced dehydration alters enzymatic and non-enzymatic components of the antioxidant defense system in Nepeta nervosa Royle ex Bentham." in Horticulturae, 9, no. 12 (2023):1277,
https://doi.org/10.3390/horticulturae9121277 . .

TY  - CONF
AU  - Marković, Tijana
AU  - Aničić, Neda
AU  - Marković Hadžić, Stefan
AU  - Božunović, Jelena
AU  - Dmitrović, Slavica
AU  - Nestorović Živković, Jasmina
AU  - Gašić, Uroš
AU  - Milutinović, Milica
AU  - Petrović, Luka
AU  - Mišić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5571
AB  - Because of their sessile lifestyle, plants have been constantly facing ultraviolet (UV-B) radiation,
a type of abiotic stress which affects their overall growth, development, physiology, and metabolism. Qualitative and quantitative content of specialized metabolites, chemical compounds that
allow plants to adapt to specific conditions of the environment, is also affected. Nepeta nuda L.
(fam. Lamiaceae) is characterized by a significant amount of iridoids, monoterpenoid compounds
with prominent pharmacological effects. The aim of this study was to detect changes in the content of major iridoids (1,5,9-epideoxyloganic acid (1,5,9-eDLA), nepetalactone (NL), and dehydronepetalactone (DNL)) in N. nuda plants after exposure to UV-B radiation (during 30 and 60 min),
in parallel with expression analysis of genes participating in the biosynthetic pathway of iridoids
(NnGAPDH, NnGPPS, NnGES, NnG8O, Nn8HGO, NnIS, NnNEPS). After both UV-B treatments, the gene
GPPS showed the highest increase in gene expression, relative to control plants, while the gene
G8O showed the highest decrease in expression level. At the same time, UHPLC/DAD/(±)HESI-MS2
analysis revealed an increase in the concentration of 1,5,9-eDLA and DNL following the exposure
of plants to UV-B radiation for 60 minutes. In the case of a 30-minute treatment, there was an
increase in the concentration of eDLA, while the concentration of DNL was reduced, relative to
non-treated plants. The present study brings novel insights into the molecular background of
iridoid biosynthesis in UV-B stressed plants, which could help in optimizing the elicitation procedures and thus accumulation of bioactive compounds in N. nuda.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - UV-B radiation-elicitated biosynthesis of iridoids in leaves of in vitro grown Nepeta nuda L.
SP  - 85
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5571
ER  - 

@conference{
author = "Marković, Tijana and Aničić, Neda and Marković Hadžić, Stefan and Božunović, Jelena and Dmitrović, Slavica and Nestorović Živković, Jasmina and Gašić, Uroš and Milutinović, Milica and Petrović, Luka and Mišić, Danijela",
year = "2022",
abstract = "Because of their sessile lifestyle, plants have been constantly facing ultraviolet (UV-B) radiation,
a type of abiotic stress which affects their overall growth, development, physiology, and metabolism. Qualitative and quantitative content of specialized metabolites, chemical compounds that
allow plants to adapt to specific conditions of the environment, is also affected. Nepeta nuda L.
(fam. Lamiaceae) is characterized by a significant amount of iridoids, monoterpenoid compounds
with prominent pharmacological effects. The aim of this study was to detect changes in the content of major iridoids (1,5,9-epideoxyloganic acid (1,5,9-eDLA), nepetalactone (NL), and dehydronepetalactone (DNL)) in N. nuda plants after exposure to UV-B radiation (during 30 and 60 min),
in parallel with expression analysis of genes participating in the biosynthetic pathway of iridoids
(NnGAPDH, NnGPPS, NnGES, NnG8O, Nn8HGO, NnIS, NnNEPS). After both UV-B treatments, the gene
GPPS showed the highest increase in gene expression, relative to control plants, while the gene
G8O showed the highest decrease in expression level. At the same time, UHPLC/DAD/(±)HESI-MS2
analysis revealed an increase in the concentration of 1,5,9-eDLA and DNL following the exposure
of plants to UV-B radiation for 60 minutes. In the case of a 30-minute treatment, there was an
increase in the concentration of eDLA, while the concentration of DNL was reduced, relative to
non-treated plants. The present study brings novel insights into the molecular background of
iridoid biosynthesis in UV-B stressed plants, which could help in optimizing the elicitation procedures and thus accumulation of bioactive compounds in N. nuda.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "UV-B radiation-elicitated biosynthesis of iridoids in leaves of in vitro grown Nepeta nuda L.",
pages = "85",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5571"
}

Marković, T., Aničić, N., Marković Hadžić, S., Božunović, J., Dmitrović, S., Nestorović Živković, J., Gašić, U., Milutinović, M., Petrović, L.,& Mišić, D.. (2022). UV-B radiation-elicitated biosynthesis of iridoids in leaves of in vitro grown Nepeta nuda L.. in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 85.
https://hdl.handle.net/21.15107/rcub_ibiss_5571

Marković T, Aničić N, Marković Hadžić S, Božunović J, Dmitrović S, Nestorović Živković J, Gašić U, Milutinović M, Petrović L, Mišić D. UV-B radiation-elicitated biosynthesis of iridoids in leaves of in vitro grown Nepeta nuda L.. in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:85.
https://hdl.handle.net/21.15107/rcub_ibiss_5571 .

Marković, Tijana, Aničić, Neda, Marković Hadžić, Stefan, Božunović, Jelena, Dmitrović, Slavica, Nestorović Živković, Jasmina, Gašić, Uroš, Milutinović, Milica, Petrović, Luka, Mišić, Danijela, "UV-B radiation-elicitated biosynthesis of iridoids in leaves of in vitro grown Nepeta nuda L." in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):85,
https://hdl.handle.net/21.15107/rcub_ibiss_5571 .

TY  - CONF
AU  - Marković Hadžić, Stefan
AU  - Božunović, Jelena
AU  - Marković, Tijana
AU  - Aničić, Neda
AU  - Gašić, Uroš
AU  - Mišić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6568
AB  - Studies focusing on plant specialized metabolism and enhanced production of bioactive compounds in a controlled environment are of increasing interest bearing in mind that some of those compounds are widely used. Centaurium erythraea Rafn produces a plethora of specialized metabolites among which secoiridoid glucosides predominate. Secoiridoids in plants serve as defense compounds against herbivores and microorganisms, which makes them highly attractive
for agricultural and pharmaceutical applications. In plants, ambient UV–B radiation has been suggested to prime protective responses towards various stressors. The overall goal of our research
was to reveal the effect of short-term UV–B light exposure (30 and 60 minutes) on secoiridoid
metabolism in leaves of common centaury grown in vitro. In order to achieve this goal, expression analysis of genes involved in the secoiridoid biosynthetic path-way was performed in parallel
with UHPLC/(–)HESI–MS2 metabolic profiling of leaves. Centaury plants exposed to a UV–B light
for 60 minutes displayed prominent discoloration, indicating possible chlorophyll degradation,
or its reduced synthesis. Gene expression analysis of nine secoiridoid biosynthesis-related genes
(CeGPPS, CeGES, CeG80, Ce8HGO, CeIS, CeIO, Ce7DGLT, Ce7DLH, CeSLS) was monitored. Following the UV–B treatment, a statistically significant decrease in relative gene expression was detected only for CeG80, while metabolic profiling revealed elevated accumulation of sweroside, swertiamarin,
and gentiopicrin in both 30 and 60 min UV–B-treated centaury plants. These results suggest that
UV–B light exposure can be used to stimulate accumulation of secoiridoids in centaury plants for
possible extraction and application in pharmacy, agriculture and food industry.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Effect of UV - B radiation on secoiridoids production in Centaurium erythraea Rafn leaves
SP  - 127
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6568
ER  - 

@conference{
author = "Marković Hadžić, Stefan and Božunović, Jelena and Marković, Tijana and Aničić, Neda and Gašić, Uroš and Mišić, Danijela",
year = "2022",
abstract = "Studies focusing on plant specialized metabolism and enhanced production of bioactive compounds in a controlled environment are of increasing interest bearing in mind that some of those compounds are widely used. Centaurium erythraea Rafn produces a plethora of specialized metabolites among which secoiridoid glucosides predominate. Secoiridoids in plants serve as defense compounds against herbivores and microorganisms, which makes them highly attractive
for agricultural and pharmaceutical applications. In plants, ambient UV–B radiation has been suggested to prime protective responses towards various stressors. The overall goal of our research
was to reveal the effect of short-term UV–B light exposure (30 and 60 minutes) on secoiridoid
metabolism in leaves of common centaury grown in vitro. In order to achieve this goal, expression analysis of genes involved in the secoiridoid biosynthetic path-way was performed in parallel
with UHPLC/(–)HESI–MS2 metabolic profiling of leaves. Centaury plants exposed to a UV–B light
for 60 minutes displayed prominent discoloration, indicating possible chlorophyll degradation,
or its reduced synthesis. Gene expression analysis of nine secoiridoid biosynthesis-related genes
(CeGPPS, CeGES, CeG80, Ce8HGO, CeIS, CeIO, Ce7DGLT, Ce7DLH, CeSLS) was monitored. Following the UV–B treatment, a statistically significant decrease in relative gene expression was detected only for CeG80, while metabolic profiling revealed elevated accumulation of sweroside, swertiamarin,
and gentiopicrin in both 30 and 60 min UV–B-treated centaury plants. These results suggest that
UV–B light exposure can be used to stimulate accumulation of secoiridoids in centaury plants for
possible extraction and application in pharmacy, agriculture and food industry.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Effect of UV - B radiation on secoiridoids production in Centaurium erythraea Rafn leaves",
pages = "127",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6568"
}

Marković Hadžić, S., Božunović, J., Marković, T., Aničić, N., Gašić, U.,& Mišić, D.. (2022). Effect of UV - B radiation on secoiridoids production in Centaurium erythraea Rafn leaves. in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 127.
https://hdl.handle.net/21.15107/rcub_ibiss_6568

Marković Hadžić S, Božunović J, Marković T, Aničić N, Gašić U, Mišić D. Effect of UV - B radiation on secoiridoids production in Centaurium erythraea Rafn leaves. in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:127.
https://hdl.handle.net/21.15107/rcub_ibiss_6568 .

Marković Hadžić, Stefan, Božunović, Jelena, Marković, Tijana, Aničić, Neda, Gašić, Uroš, Mišić, Danijela, "Effect of UV - B radiation on secoiridoids production in Centaurium erythraea Rafn leaves" in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):127,
https://hdl.handle.net/21.15107/rcub_ibiss_6568 .