TY  - JOUR
AU  - Mandić, Marija R.
AU  - Oalđe, Mariana M.
AU  - Lunić, Tanja M.
AU  - Sabovljević, Aneta D.
AU  - Sabovljević, Marko S.
AU  - Gašić, Uroš
AU  - Duletić-Laušević, Sonja N.
AU  - Božić, Bojan Đ.
AU  - Božić Nedeljković, Biljana Dj.
AU  - Chen, Jen-Tsung
PY  - 2021
UR  - internal-pdf://Mandić et al. - 2021 - Chemical characterization and in vitro immunomodulatory effects of different extracts of moss Hedwigia ciliata (H.pdf
UR  - https://dx.plos.org/10.1371/journal.pone.0246810
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4156
AB  - Bioactive compounds from natural sources are of great importance because of their potential pharmacological activity and tremendous structural diversity. In this study, the chemical composition of different moss extracts of Hedwigia ciliata P. Beauv. have been examined, as well as their antioxidant, antineurodegenerative/anti-neuroinflammatory, antidiabetic, and antiproliferative potential. The extracts were prepared by Soxhlet extractor using solvents of different polarity. Chemical characterization of the extracts revealed the presence of phenolics and flavonoid compounds, together with triterpenoids as secondary metabolites of high biological activity. Significant antioxidant properties of all the extracts were exhibited using the β-carotene assay. The highest activities were found for water:ethanol extract (with the highest inhibition rate of 96%), but also significant inhibition was measured for ethanol and ethyl acetate extracts (80% and 70%, respectively). Confirmation of biocompatibility of investigated moss extracts has been performed using normal human fibroblast cell line, MRC-5. The H . ciliata extracts exhibited significant antiproliferative activity (~ 50%) against the MDA-MB-231 (human breast adenocarcinoma cell line), which has not previously been reported elsewhere. The Griess assay confirmed the potential anti-neuroinflammatory activity of the extracts, as significant effects in reducing NO production by LPS-stimulated BV2 (normal murine microglia cell line) was observed. This data is in line with noted antineurodegenerative potential measured by the inhibition of acetylcholinesterase (with the highest inhibition rate of 60% for ethyl acetate extract) and tyrosinase (with the highest inhibition rate of 70% for ethanol extract). Additionally, the H . ciliata extracts exhibited significant antidiabetic effect mediated by α-glucosidase inhibition (with the highest inhibition rate of 80% for ethyl acetate extract). The obtained data suggest the presence of immunomodulatory effects of the moss extracts in vitro , which allows the design of new experiments aimed at detecting and characterizing bioactive compounds of the extracts and additionally elucidate detailed mechanisms of their effects.
PB  - Public Library of Science (PLoS)
T2  - PLOS ONE
T1  - Chemical characterization and in vitro immunomodulatory effects of different extracts of moss Hedwigia ciliata (Hedw.) P. Beauv. from the Vršačke Planine Mts., Serbia
IS  - 2
VL  - 16
DO  - 10.1371/journal.pone.0246810
SP  - e0246810
ER  - 

@article{
author = "Mandić, Marija R. and Oalđe, Mariana M. and Lunić, Tanja M. and Sabovljević, Aneta D. and Sabovljević, Marko S. and Gašić, Uroš and Duletić-Laušević, Sonja N. and Božić, Bojan Đ. and Božić Nedeljković, Biljana Dj. and Chen, Jen-Tsung",
year = "2021",
abstract = "Bioactive compounds from natural sources are of great importance because of their potential pharmacological activity and tremendous structural diversity. In this study, the chemical composition of different moss extracts of Hedwigia ciliata P. Beauv. have been examined, as well as their antioxidant, antineurodegenerative/anti-neuroinflammatory, antidiabetic, and antiproliferative potential. The extracts were prepared by Soxhlet extractor using solvents of different polarity. Chemical characterization of the extracts revealed the presence of phenolics and flavonoid compounds, together with triterpenoids as secondary metabolites of high biological activity. Significant antioxidant properties of all the extracts were exhibited using the β-carotene assay. The highest activities were found for water:ethanol extract (with the highest inhibition rate of 96%), but also significant inhibition was measured for ethanol and ethyl acetate extracts (80% and 70%, respectively). Confirmation of biocompatibility of investigated moss extracts has been performed using normal human fibroblast cell line, MRC-5. The H . ciliata extracts exhibited significant antiproliferative activity (~ 50%) against the MDA-MB-231 (human breast adenocarcinoma cell line), which has not previously been reported elsewhere. The Griess assay confirmed the potential anti-neuroinflammatory activity of the extracts, as significant effects in reducing NO production by LPS-stimulated BV2 (normal murine microglia cell line) was observed. This data is in line with noted antineurodegenerative potential measured by the inhibition of acetylcholinesterase (with the highest inhibition rate of 60% for ethyl acetate extract) and tyrosinase (with the highest inhibition rate of 70% for ethanol extract). Additionally, the H . ciliata extracts exhibited significant antidiabetic effect mediated by α-glucosidase inhibition (with the highest inhibition rate of 80% for ethyl acetate extract). The obtained data suggest the presence of immunomodulatory effects of the moss extracts in vitro , which allows the design of new experiments aimed at detecting and characterizing bioactive compounds of the extracts and additionally elucidate detailed mechanisms of their effects.",
publisher = "Public Library of Science (PLoS)",
journal = "PLOS ONE",
title = "Chemical characterization and in vitro immunomodulatory effects of different extracts of moss Hedwigia ciliata (Hedw.) P. Beauv. from the Vršačke Planine Mts., Serbia",
number = "2",
volume = "16",
doi = "10.1371/journal.pone.0246810",
pages = "e0246810"
}

Mandić, M. R., Oalđe, M. M., Lunić, T. M., Sabovljević, A. D., Sabovljević, M. S., Gašić, U., Duletić-Laušević, S. N., Božić, B. Đ., Božić Nedeljković, B. Dj.,& Chen, J.. (2021). Chemical characterization and in vitro immunomodulatory effects of different extracts of moss Hedwigia ciliata (Hedw.) P. Beauv. from the Vršačke Planine Mts., Serbia. in PLOS ONE
Public Library of Science (PLoS)., 16(2), e0246810.
https://doi.org/10.1371/journal.pone.0246810

Mandić MR, Oalđe MM, Lunić TM, Sabovljević AD, Sabovljević MS, Gašić U, Duletić-Laušević SN, Božić BĐ, Božić Nedeljković BD, Chen J. Chemical characterization and in vitro immunomodulatory effects of different extracts of moss Hedwigia ciliata (Hedw.) P. Beauv. from the Vršačke Planine Mts., Serbia. in PLOS ONE. 2021;16(2):e0246810.
doi:10.1371/journal.pone.0246810 .

Mandić, Marija R., Oalđe, Mariana M., Lunić, Tanja M., Sabovljević, Aneta D., Sabovljević, Marko S., Gašić, Uroš, Duletić-Laušević, Sonja N., Božić, Bojan Đ., Božić Nedeljković, Biljana Dj., Chen, Jen-Tsung, "Chemical characterization and in vitro immunomodulatory effects of different extracts of moss Hedwigia ciliata (Hedw.) P. Beauv. from the Vršačke Planine Mts., Serbia" in PLOS ONE, 16, no. 2 (2021):e0246810,
https://doi.org/10.1371/journal.pone.0246810 . .