TY  - JOUR
AU  - Senizza, Biancamaria
AU  - Rocchetti, Gabriele
AU  - Sinan, Kouadio Ibrahime
AU  - Zengin, Gokhan
AU  - Mahomoodally, Mohamad Fawzi
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Lobine, Devina
AU  - Etienne, Ouattara Katinan
AU  - Lucini, Luigi
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4171
AB  - Solanum erianthum D. Don. and S. torvum Swartz of the family Solanaceae have been extensively used for both food and pharmaceutical purposes. In this study, a metabolomic approach was used to compare the phytochemical composition and biological activity of the different plant parts of both species. The inhibitory activity against important enzymes (i.e., cholinesterases, tyrosinase, α-amylase and α-glucosidase), antimicrobial and antifungal potentials, in vitro antioxidant activity, as well as the phenolic and alkaloid profiles of the fruit, leaf and stem bark extracts of both species were studied. The results showed that the stem barks were characterized by the highest amounts of total phenolics (on average: 12.6 mg/g), whilst the S. erianthum leaves were the best source of total alkaloids (2.1 mg/g). Overall, the stem bark extracts of both species were found to be a good source of antioxidant compounds. All the extracts were effective against acetylcholinesterase and tyrosinase, while only the leaf extracts of both plants were a good source of butyrylcholinesterase inhibitors. The extracts have significant anti-microbial and anti-fungal potentials. A modest anti-diabetic effect was measured for α-amylase and α-glucosidase. Strong correlations (p <0.01) were found between the bioactives and the biological activities. Therefore, this study showed the potential of untargeted metabolomics to investigate the bioactive profile of plant extracts, thus providing discrimination between S. erianthum and S. torvum.
PB  - Elsevier Ltd
T2  - Food Bioscience
T1  - The phenolic and alkaloid profiles of Solanum erianthum and Solanum torvum modulated their biological properties
VL  - 41
DO  - 10.1016/j.fbio.2021.100974
SP  - 100974
ER  - 

@article{
author = "Senizza, Biancamaria and Rocchetti, Gabriele and Sinan, Kouadio Ibrahime and Zengin, Gokhan and Mahomoodally, Mohamad Fawzi and Glamočlija, Jasmina and Soković, Marina and Lobine, Devina and Etienne, Ouattara Katinan and Lucini, Luigi",
year = "2021",
abstract = "Solanum erianthum D. Don. and S. torvum Swartz of the family Solanaceae have been extensively used for both food and pharmaceutical purposes. In this study, a metabolomic approach was used to compare the phytochemical composition and biological activity of the different plant parts of both species. The inhibitory activity against important enzymes (i.e., cholinesterases, tyrosinase, α-amylase and α-glucosidase), antimicrobial and antifungal potentials, in vitro antioxidant activity, as well as the phenolic and alkaloid profiles of the fruit, leaf and stem bark extracts of both species were studied. The results showed that the stem barks were characterized by the highest amounts of total phenolics (on average: 12.6 mg/g), whilst the S. erianthum leaves were the best source of total alkaloids (2.1 mg/g). Overall, the stem bark extracts of both species were found to be a good source of antioxidant compounds. All the extracts were effective against acetylcholinesterase and tyrosinase, while only the leaf extracts of both plants were a good source of butyrylcholinesterase inhibitors. The extracts have significant anti-microbial and anti-fungal potentials. A modest anti-diabetic effect was measured for α-amylase and α-glucosidase. Strong correlations (p <0.01) were found between the bioactives and the biological activities. Therefore, this study showed the potential of untargeted metabolomics to investigate the bioactive profile of plant extracts, thus providing discrimination between S. erianthum and S. torvum.",
publisher = "Elsevier Ltd",
journal = "Food Bioscience",
title = "The phenolic and alkaloid profiles of Solanum erianthum and Solanum torvum modulated their biological properties",
volume = "41",
doi = "10.1016/j.fbio.2021.100974",
pages = "100974"
}

Senizza, B., Rocchetti, G., Sinan, K. I., Zengin, G., Mahomoodally, M. F., Glamočlija, J., Soković, M., Lobine, D., Etienne, O. K.,& Lucini, L.. (2021). The phenolic and alkaloid profiles of Solanum erianthum and Solanum torvum modulated their biological properties. in Food Bioscience
Elsevier Ltd., 41, 100974.
https://doi.org/10.1016/j.fbio.2021.100974

Senizza B, Rocchetti G, Sinan KI, Zengin G, Mahomoodally MF, Glamočlija J, Soković M, Lobine D, Etienne OK, Lucini L. The phenolic and alkaloid profiles of Solanum erianthum and Solanum torvum modulated their biological properties. in Food Bioscience. 2021;41:100974.
doi:10.1016/j.fbio.2021.100974 .

Senizza, Biancamaria, Rocchetti, Gabriele, Sinan, Kouadio Ibrahime, Zengin, Gokhan, Mahomoodally, Mohamad Fawzi, Glamočlija, Jasmina, Soković, Marina, Lobine, Devina, Etienne, Ouattara Katinan, Lucini, Luigi, "The phenolic and alkaloid profiles of Solanum erianthum and Solanum torvum modulated their biological properties" in Food Bioscience, 41 (2021):100974,
https://doi.org/10.1016/j.fbio.2021.100974 . .

TY  - JOUR
AU  - Rocchetti, Gabriele
AU  - Zengin, Gokhan
AU  - Cakmak, Yavuz Selim
AU  - Mahomoodally, Mohamad Fawzi
AU  - Kaya, Muhammed Fettah
AU  - Alsheikh, Sarah Mohammed
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Lobine, Devina
AU  - Lucini, Luigi
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/123456789/3907
AB  - The genus Consolida is one of the most important within the Ranunculaceae family and several members of this genus contain important biologically active compounds, such as phenolics and alkaloids. The present study attempts for the first time to assess the biological properties and phytochemical constituents of six Consolida species (C. glandulosa (Boiss. & A. Huet) Bornm, C. hellospontica (Boiss.) Chater, C. raveyi (Boiss.) Schrödinger, C. regalis (Boiss.) Schrödinger, C. staminosa P.H. Davis & Sorger and C. stenocarpa (P.H. Davis & M. Hossain) P.H. Davis) growing in Turkey. A comprehensive phytochemical profiling of the different species was achieved by using an ultra-high-performance liquid chromatography-quadrupole time-of-flight (UHPLC-QTOF) mass spectrometry, targeting polyphenols and diterpene alkaloids. Also, the in vitro antioxidant assays (2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS), ferric reducing antioxidant power (FRAP), cupric reducing antioxidant capacity (CUPRAC), metal chelating and phosphomolybdenum) and enzyme inhibition (cholinesterases, tyrosinase, α-amylase, and α-glucosidase) potentials of the different Consolida methanolic extracts were evaluated. The UHPLC-QTOF profiling revealed 492 compounds, being 236 flavonoids, 93 phenolic acids, 78 tyrosol derivatives, 49 diterpene alkaloids, 29 lignans, and 7 stilbenes. The cumulative phenolic content ranged from 17.65 mg Eq./g for C. staminosa up to 43.04 mg Eq./g for C. glandulosa, whilst the total diterpene alkaloids content was exclusively found in C. glandulosa extracts (3.53 mg Eq./g). Consolida regalis (58. 61 mg trolox equivalent (TE)/g for DPPH and 65.38 mg TE/g for ABTS) and C. stenocarpa (46.81 mg TE/g for DPPH and 78.12 mg TE/g for ABTS) exhibited the highest anti-radical abilities, while the best reducing power values were recorded for C. glandulosa (129.46 mg TE/g for CUPRAC and 77.01 mg TE/g for FRAP) and C. raveyi (124.56 mg TE/g for CUPRAC and 78.36 mg TE/g for FRAP). Also, following the cholinesterases inhibition assays, C. hellospontica and C. glandulosa exhibited the highest inhibitory effects. Regarding tyrosinase inhibitory effects, C. raveyi showed the highest value, being 126.60 mg kojic acid equivalent (KAE)/g, followed by C. glandulosa (125.82 mg KAE/g) and C. stenocarpa (123.09 mg KAE/g). Besides, Consolida staminosa showed potential inhibition against α-amylase, but low glucosidase inhibitory properties. Therefore, the present findings could provide a starting point for further investigations to promote the industrial exploitation of these species with the aim of designing novel phyto-pharmaceuticals.
PB  - Elsevier B.V.
T2  - Industrial Crops and Products
T1  - A UHPLC-QTOF-MS screening provides new insights into the phytochemical composition and biological properties of six Consolida species from Turkey
VL  - 158
DO  - 10.1016/j.indcrop.2020.112966
SP  - 112966
ER  - 

@article{
author = "Rocchetti, Gabriele and Zengin, Gokhan and Cakmak, Yavuz Selim and Mahomoodally, Mohamad Fawzi and Kaya, Muhammed Fettah and Alsheikh, Sarah Mohammed and Glamočlija, Jasmina and Soković, Marina and Lobine, Devina and Lucini, Luigi",
year = "2020",
abstract = "The genus Consolida is one of the most important within the Ranunculaceae family and several members of this genus contain important biologically active compounds, such as phenolics and alkaloids. The present study attempts for the first time to assess the biological properties and phytochemical constituents of six Consolida species (C. glandulosa (Boiss. & A. Huet) Bornm, C. hellospontica (Boiss.) Chater, C. raveyi (Boiss.) Schrödinger, C. regalis (Boiss.) Schrödinger, C. staminosa P.H. Davis & Sorger and C. stenocarpa (P.H. Davis & M. Hossain) P.H. Davis) growing in Turkey. A comprehensive phytochemical profiling of the different species was achieved by using an ultra-high-performance liquid chromatography-quadrupole time-of-flight (UHPLC-QTOF) mass spectrometry, targeting polyphenols and diterpene alkaloids. Also, the in vitro antioxidant assays (2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS), ferric reducing antioxidant power (FRAP), cupric reducing antioxidant capacity (CUPRAC), metal chelating and phosphomolybdenum) and enzyme inhibition (cholinesterases, tyrosinase, α-amylase, and α-glucosidase) potentials of the different Consolida methanolic extracts were evaluated. The UHPLC-QTOF profiling revealed 492 compounds, being 236 flavonoids, 93 phenolic acids, 78 tyrosol derivatives, 49 diterpene alkaloids, 29 lignans, and 7 stilbenes. The cumulative phenolic content ranged from 17.65 mg Eq./g for C. staminosa up to 43.04 mg Eq./g for C. glandulosa, whilst the total diterpene alkaloids content was exclusively found in C. glandulosa extracts (3.53 mg Eq./g). Consolida regalis (58. 61 mg trolox equivalent (TE)/g for DPPH and 65.38 mg TE/g for ABTS) and C. stenocarpa (46.81 mg TE/g for DPPH and 78.12 mg TE/g for ABTS) exhibited the highest anti-radical abilities, while the best reducing power values were recorded for C. glandulosa (129.46 mg TE/g for CUPRAC and 77.01 mg TE/g for FRAP) and C. raveyi (124.56 mg TE/g for CUPRAC and 78.36 mg TE/g for FRAP). Also, following the cholinesterases inhibition assays, C. hellospontica and C. glandulosa exhibited the highest inhibitory effects. Regarding tyrosinase inhibitory effects, C. raveyi showed the highest value, being 126.60 mg kojic acid equivalent (KAE)/g, followed by C. glandulosa (125.82 mg KAE/g) and C. stenocarpa (123.09 mg KAE/g). Besides, Consolida staminosa showed potential inhibition against α-amylase, but low glucosidase inhibitory properties. Therefore, the present findings could provide a starting point for further investigations to promote the industrial exploitation of these species with the aim of designing novel phyto-pharmaceuticals.",
publisher = "Elsevier B.V.",
journal = "Industrial Crops and Products",
title = "A UHPLC-QTOF-MS screening provides new insights into the phytochemical composition and biological properties of six Consolida species from Turkey",
volume = "158",
doi = "10.1016/j.indcrop.2020.112966",
pages = "112966"
}

Rocchetti, G., Zengin, G., Cakmak, Y. S., Mahomoodally, M. F., Kaya, M. F., Alsheikh, S. M., Glamočlija, J., Soković, M., Lobine, D.,& Lucini, L.. (2020). A UHPLC-QTOF-MS screening provides new insights into the phytochemical composition and biological properties of six Consolida species from Turkey. in Industrial Crops and Products
Elsevier B.V.., 158, 112966.
https://doi.org/10.1016/j.indcrop.2020.112966

Rocchetti G, Zengin G, Cakmak YS, Mahomoodally MF, Kaya MF, Alsheikh SM, Glamočlija J, Soković M, Lobine D, Lucini L. A UHPLC-QTOF-MS screening provides new insights into the phytochemical composition and biological properties of six Consolida species from Turkey. in Industrial Crops and Products. 2020;158:112966.
doi:10.1016/j.indcrop.2020.112966 .

Rocchetti, Gabriele, Zengin, Gokhan, Cakmak, Yavuz Selim, Mahomoodally, Mohamad Fawzi, Kaya, Muhammed Fettah, Alsheikh, Sarah Mohammed, Glamočlija, Jasmina, Soković, Marina, Lobine, Devina, Lucini, Luigi, "A UHPLC-QTOF-MS screening provides new insights into the phytochemical composition and biological properties of six Consolida species from Turkey" in Industrial Crops and Products, 158 (2020):112966,
https://doi.org/10.1016/j.indcrop.2020.112966 . .

TY  - JOUR
AU  - Uysal, Sengul
AU  - Senkardes, Ismail
AU  - Mollica, Adriano
AU  - Zengin, Gokhan
AU  - Bulut, Gizem
AU  - Dogan, Ahmet
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Lobine, Devina
AU  - Mahomoodally, Fawzi M
PY  - 2019
UR  - https://www.tandfonline.com/doi/full/10.1080/07391102.2018.1506361
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3194
AB  - Tragopogon dubius and Tussilago farfara are consumed as vegetables and used in folk medicine to manage common diseases. Herein, the chemical compositions and biological activities of different leaf extracts (ethyl acetate, methanol, and water) of T. dubius and T. farfara were evaluated. The antibacterial, antifungal, and antioxidant abilities of the extracts were tested using different assays including free radical scavenging, reducing power, phosphomolybdenum, and metal chelating assays. Enzyme inhibitory potentials were evaluated against cholinesterases, tyrosinase, α-amylase and α-glucosidase. Complexes of bioactive compounds (chlorogenic and rosmarinic acid) were docked into the enzymatic cavity of α-glucosidase and subjected to molecular dynamic calculation, enzyme conformational stability, and flexibility analysis. T. dubius and T. farfara extracts showed remarkable antioxidant potentials. Ethyl acetate extracts of T. dubius and T. farfara were the most potent inhibitors of acetylcholinesterase and butyrylcholinesterase. T. dubius ethyl acetate extract and T. farfara methanolic extract showed noteworthy activity against α-glucosidase. High performance liquid chromatography analysis revealed the abundance of some phenolic compounds including chlorogenic and rosmarinic acids. Ethyl acetate extract of T. dubius showed notable antifungal activity against all strains. Docking studies showed best pose for chlorogenic acid was stabilized by a network of hydrogen bonds with residues Asp1157, Asp1279, whereas rosmarinic acid showed several hydrogen bonds with Asp1157, Asp1420, Asp1526, Lys1460 and Trp1369. This study further validates the use of T. dubius and T. farfara in traditional medicine, as well as act as a stimulus for further studies for future biomedicine development. Communicated by Ramaswamy H. Sarma.
T2  - Journal of Biomolecular Structure and Dynamics
T1  - Biologically active compounds from two members of the Asteraceae family: Tragopogon dubius Scop. and Tussilago farfara L.
IS  - 12
VL  - 37
DO  - 10.1080/07391102.2018.1506361
SP  - 3269
EP  - 3281
ER  - 

@article{
author = "Uysal, Sengul and Senkardes, Ismail and Mollica, Adriano and Zengin, Gokhan and Bulut, Gizem and Dogan, Ahmet and Glamočlija, Jasmina and Soković, Marina and Lobine, Devina and Mahomoodally, Fawzi M",
year = "2019",
abstract = "Tragopogon dubius and Tussilago farfara are consumed as vegetables and used in folk medicine to manage common diseases. Herein, the chemical compositions and biological activities of different leaf extracts (ethyl acetate, methanol, and water) of T. dubius and T. farfara were evaluated. The antibacterial, antifungal, and antioxidant abilities of the extracts were tested using different assays including free radical scavenging, reducing power, phosphomolybdenum, and metal chelating assays. Enzyme inhibitory potentials were evaluated against cholinesterases, tyrosinase, α-amylase and α-glucosidase. Complexes of bioactive compounds (chlorogenic and rosmarinic acid) were docked into the enzymatic cavity of α-glucosidase and subjected to molecular dynamic calculation, enzyme conformational stability, and flexibility analysis. T. dubius and T. farfara extracts showed remarkable antioxidant potentials. Ethyl acetate extracts of T. dubius and T. farfara were the most potent inhibitors of acetylcholinesterase and butyrylcholinesterase. T. dubius ethyl acetate extract and T. farfara methanolic extract showed noteworthy activity against α-glucosidase. High performance liquid chromatography analysis revealed the abundance of some phenolic compounds including chlorogenic and rosmarinic acids. Ethyl acetate extract of T. dubius showed notable antifungal activity against all strains. Docking studies showed best pose for chlorogenic acid was stabilized by a network of hydrogen bonds with residues Asp1157, Asp1279, whereas rosmarinic acid showed several hydrogen bonds with Asp1157, Asp1420, Asp1526, Lys1460 and Trp1369. This study further validates the use of T. dubius and T. farfara in traditional medicine, as well as act as a stimulus for further studies for future biomedicine development. Communicated by Ramaswamy H. Sarma.",
journal = "Journal of Biomolecular Structure and Dynamics",
title = "Biologically active compounds from two members of the Asteraceae family: Tragopogon dubius Scop. and Tussilago farfara L.",
number = "12",
volume = "37",
doi = "10.1080/07391102.2018.1506361",
pages = "3269-3281"
}

Uysal, S., Senkardes, I., Mollica, A., Zengin, G., Bulut, G., Dogan, A., Glamočlija, J., Soković, M., Lobine, D.,& Mahomoodally, F. M.. (2019). Biologically active compounds from two members of the Asteraceae family: Tragopogon dubius Scop. and Tussilago farfara L.. in Journal of Biomolecular Structure and Dynamics, 37(12), 3269-3281.
https://doi.org/10.1080/07391102.2018.1506361

Uysal S, Senkardes I, Mollica A, Zengin G, Bulut G, Dogan A, Glamočlija J, Soković M, Lobine D, Mahomoodally FM. Biologically active compounds from two members of the Asteraceae family: Tragopogon dubius Scop. and Tussilago farfara L.. in Journal of Biomolecular Structure and Dynamics. 2019;37(12):3269-3281.
doi:10.1080/07391102.2018.1506361 .

Uysal, Sengul, Senkardes, Ismail, Mollica, Adriano, Zengin, Gokhan, Bulut, Gizem, Dogan, Ahmet, Glamočlija, Jasmina, Soković, Marina, Lobine, Devina, Mahomoodally, Fawzi M, "Biologically active compounds from two members of the Asteraceae family: Tragopogon dubius Scop. and Tussilago farfara L." in Journal of Biomolecular Structure and Dynamics, 37, no. 12 (2019):3269-3281,
https://doi.org/10.1080/07391102.2018.1506361 . .