TY  - JOUR
AU  - Silva, Ana Rita
AU  - Ayuso, Manuel
AU  - Pereira, Carla
AU  - Dias, Maria Inês
AU  - Kostić, Marina
AU  - Calhelha, Ricardo C.
AU  - Soković, Marina
AU  - García, Pablo A.
AU  - Ferreira, Isabel C.F.R.
AU  - Barros, Lillian
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0926669021011080
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4746
AB  - Cytinus hypocistis (L.) L. is a comestible holoparasite with great potential for cosmeceutical application. Although its high tannin content has been associated with its bioactive and inhibitory enzyme properties, this is the first report establishing a relationship between parasite and host (Halimium lasianthum (Lam.) Greuter) phenolic profile and bioactive properties. Thus, five extracts (aerial and root extracts of non-parasited and parasited H. lasianthum and C. hypocistis) were evaluated. The tentative identification of both species comprises 39 phenolic compounds. Hydrolysable tannins and flavonoids were the main identified groups in C. hypocistis and H. lasianthum extracts, respectively. Regarding bioactivities, C. hypocistis exhibited excellent antioxidant results both in Oxidative Haemolysis (OxHLIA) and inhibition of Thiobarbituric Acid Reactive Substances Formation (TBARS). The tested extracts presented antimicrobial inhibition, anti-inflammatory activity, and effective cytotoxicity against tumour cells. C. hypocistis exhibited the lowest cytotoxicity on a non-tumour cell line. Principal Component Analysis (PCA) was a suitable approach to analyse differences among samples, explaining up to 67% of data variability and suggesting no similarities between parasite and host phenolic composition and bioactivities. Therefore, this comparative study emphasises the significance of both species as a source of biologically active compounds.
PB  - Amsterdam: Elsevier B.V.
T2  - Industrial Crops and Products
T1  - Evaluation of parasite and host phenolic composition and bioactivities − The Practical Case of Cytinus hypocistis (L.) L. and Halimium lasianthum (Lam.) Greuter
VL  - 176
DO  - 10.1016/j.indcrop.2021.114343
SP  - 114343
ER  - 

@article{
author = "Silva, Ana Rita and Ayuso, Manuel and Pereira, Carla and Dias, Maria Inês and Kostić, Marina and Calhelha, Ricardo C. and Soković, Marina and García, Pablo A. and Ferreira, Isabel C.F.R. and Barros, Lillian",
year = "2022",
abstract = "Cytinus hypocistis (L.) L. is a comestible holoparasite with great potential for cosmeceutical application. Although its high tannin content has been associated with its bioactive and inhibitory enzyme properties, this is the first report establishing a relationship between parasite and host (Halimium lasianthum (Lam.) Greuter) phenolic profile and bioactive properties. Thus, five extracts (aerial and root extracts of non-parasited and parasited H. lasianthum and C. hypocistis) were evaluated. The tentative identification of both species comprises 39 phenolic compounds. Hydrolysable tannins and flavonoids were the main identified groups in C. hypocistis and H. lasianthum extracts, respectively. Regarding bioactivities, C. hypocistis exhibited excellent antioxidant results both in Oxidative Haemolysis (OxHLIA) and inhibition of Thiobarbituric Acid Reactive Substances Formation (TBARS). The tested extracts presented antimicrobial inhibition, anti-inflammatory activity, and effective cytotoxicity against tumour cells. C. hypocistis exhibited the lowest cytotoxicity on a non-tumour cell line. Principal Component Analysis (PCA) was a suitable approach to analyse differences among samples, explaining up to 67% of data variability and suggesting no similarities between parasite and host phenolic composition and bioactivities. Therefore, this comparative study emphasises the significance of both species as a source of biologically active compounds.",
publisher = "Amsterdam: Elsevier B.V.",
journal = "Industrial Crops and Products",
title = "Evaluation of parasite and host phenolic composition and bioactivities − The Practical Case of Cytinus hypocistis (L.) L. and Halimium lasianthum (Lam.) Greuter",
volume = "176",
doi = "10.1016/j.indcrop.2021.114343",
pages = "114343"
}

Silva, A. R., Ayuso, M., Pereira, C., Dias, M. I., Kostić, M., Calhelha, R. C., Soković, M., García, P. A., Ferreira, I. C.F.R.,& Barros, L.. (2022). Evaluation of parasite and host phenolic composition and bioactivities − The Practical Case of Cytinus hypocistis (L.) L. and Halimium lasianthum (Lam.) Greuter. in Industrial Crops and Products
Amsterdam: Elsevier B.V.., 176, 114343.
https://doi.org/10.1016/j.indcrop.2021.114343

Silva AR, Ayuso M, Pereira C, Dias MI, Kostić M, Calhelha RC, Soković M, García PA, Ferreira IC, Barros L. Evaluation of parasite and host phenolic composition and bioactivities − The Practical Case of Cytinus hypocistis (L.) L. and Halimium lasianthum (Lam.) Greuter. in Industrial Crops and Products. 2022;176:114343.
doi:10.1016/j.indcrop.2021.114343 .

Silva, Ana Rita, Ayuso, Manuel, Pereira, Carla, Dias, Maria Inês, Kostić, Marina, Calhelha, Ricardo C., Soković, Marina, García, Pablo A., Ferreira, Isabel C.F.R., Barros, Lillian, "Evaluation of parasite and host phenolic composition and bioactivities − The Practical Case of Cytinus hypocistis (L.) L. and Halimium lasianthum (Lam.) Greuter" in Industrial Crops and Products, 176 (2022):114343,
https://doi.org/10.1016/j.indcrop.2021.114343 . .

TY  - JOUR
AU  - García-Pérez, Pascual
AU  - Ayuso, Manuel
AU  - Lozano-Milo, Eva
AU  - Pereira, Carla
AU  - Dias, Maria Inês
AU  - Ivanov, Marija
AU  - Calhelha, Ricardo C.
AU  - Soković, Marina
AU  - Ferreira, Isabel C.F.R.
AU  - Barros, Lillian
AU  - Gallego, Pedro P.
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4207
AB  - Bryophyllum constitutes a subgenus within the genus Kalanchoe that contains several plant species used in traditional medicine worldwide for the treatment of several diseases. However, little is known about the phytoconstituents of Bryophyllum spp. and previous reports have pointed at their low in Planta concentrations of bioactive compounds. In this work, we take advantage of plant in vitro culture for the study of the phenolic compounds found in the aerial parts of Bryophyllum spp. and their associated bioactivities. Our results show that the induction of nutritional stress leads to an improved accumulation of phenolic compounds, mainly flavonols and anthocyanins, represented by myricetin and malvidin glycosides, respectively. This effect is mainly found for B. × houghtonii, whose hydroethanolic extracts promoted the highest antioxidant, cytotoxic and anti-inflammatory activities. In the case of cytotoxic activity, Bryophyllum extracts showed an enhanced activity against the MCF-7 human breast carcinoma cell line. Meanwhile, extracts from B. daigremontianum promoted a wide range of effectiveness against different bacterial and fungal strains. This study is committed to shed light about the phytochemical potential associated to this unexplored subgenus, with the aim of considering Bryophyllum spp. as a valuable source of bioactive compounds for their exploitation in food, cosmetic and pharmaceutical industries.
PB  - Elsevier B.V.
T2  - Industrial Crops and Products
T1  - Phenolic profiling and in vitro bioactivities of three medicinal Bryophyllum plants
VL  - 162
DO  - 10.1016/j.indcrop.2021.113241
SP  - 113241
ER  - 

@article{
author = "García-Pérez, Pascual and Ayuso, Manuel and Lozano-Milo, Eva and Pereira, Carla and Dias, Maria Inês and Ivanov, Marija and Calhelha, Ricardo C. and Soković, Marina and Ferreira, Isabel C.F.R. and Barros, Lillian and Gallego, Pedro P.",
year = "2021",
abstract = "Bryophyllum constitutes a subgenus within the genus Kalanchoe that contains several plant species used in traditional medicine worldwide for the treatment of several diseases. However, little is known about the phytoconstituents of Bryophyllum spp. and previous reports have pointed at their low in Planta concentrations of bioactive compounds. In this work, we take advantage of plant in vitro culture for the study of the phenolic compounds found in the aerial parts of Bryophyllum spp. and their associated bioactivities. Our results show that the induction of nutritional stress leads to an improved accumulation of phenolic compounds, mainly flavonols and anthocyanins, represented by myricetin and malvidin glycosides, respectively. This effect is mainly found for B. × houghtonii, whose hydroethanolic extracts promoted the highest antioxidant, cytotoxic and anti-inflammatory activities. In the case of cytotoxic activity, Bryophyllum extracts showed an enhanced activity against the MCF-7 human breast carcinoma cell line. Meanwhile, extracts from B. daigremontianum promoted a wide range of effectiveness against different bacterial and fungal strains. This study is committed to shed light about the phytochemical potential associated to this unexplored subgenus, with the aim of considering Bryophyllum spp. as a valuable source of bioactive compounds for their exploitation in food, cosmetic and pharmaceutical industries.",
publisher = "Elsevier B.V.",
journal = "Industrial Crops and Products",
title = "Phenolic profiling and in vitro bioactivities of three medicinal Bryophyllum plants",
volume = "162",
doi = "10.1016/j.indcrop.2021.113241",
pages = "113241"
}

García-Pérez, P., Ayuso, M., Lozano-Milo, E., Pereira, C., Dias, M. I., Ivanov, M., Calhelha, R. C., Soković, M., Ferreira, I. C.F.R., Barros, L.,& Gallego, P. P.. (2021). Phenolic profiling and in vitro bioactivities of three medicinal Bryophyllum plants. in Industrial Crops and Products
Elsevier B.V.., 162, 113241.
https://doi.org/10.1016/j.indcrop.2021.113241

García-Pérez P, Ayuso M, Lozano-Milo E, Pereira C, Dias MI, Ivanov M, Calhelha RC, Soković M, Ferreira IC, Barros L, Gallego PP. Phenolic profiling and in vitro bioactivities of three medicinal Bryophyllum plants. in Industrial Crops and Products. 2021;162:113241.
doi:10.1016/j.indcrop.2021.113241 .

García-Pérez, Pascual, Ayuso, Manuel, Lozano-Milo, Eva, Pereira, Carla, Dias, Maria Inês, Ivanov, Marija, Calhelha, Ricardo C., Soković, Marina, Ferreira, Isabel C.F.R., Barros, Lillian, Gallego, Pedro P., "Phenolic profiling and in vitro bioactivities of three medicinal Bryophyllum plants" in Industrial Crops and Products, 162 (2021):113241,
https://doi.org/10.1016/j.indcrop.2021.113241 . .

TY  - JOUR
AU  - Ayuso, Manuel
AU  - Pinela, José
AU  - Dias, Maria Inês
AU  - Barros, Lillian
AU  - Ivanov, Marija
AU  - Calhelha, Ricardo C.
AU  - Soković, Marina
AU  - Ramil-Rego, Pablo
AU  - Barreal, M. Esther
AU  - Gallego, Pedro Pablo
AU  - Ferreira, Isabel C.F.R.
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3625
AB  - Eryngium viviparum is an endangered species that requires management efforts based on complementary ex situ conservation strategies, such as in vitro culture. This study was carried out to evaluate the phenolic composition and the antioxidant, cytotoxic, and antimicrobial properties of E. viviparum aerial and root parts obtained by this micropropagation technique. The HPLC-DAD-ESI/MSn analysis showed that phenolic compounds were more abundant in the root (102 ± 4 mg g−1 extract) than in the aerial part (40.6 ± 0.8 mg g−1 extract) of the plant. The major compound was trans rosmarinic acid, followed by trans 3-O-caffeoylquinic acid. The root extract also showed higher antioxidant activity, with a result close to that of trolox in the thiobarbituric acid reactive substances (TBARS) formation inhibition assay, and a moderate toxicity to lung (NCI-H460), breast (MCF-7) and liver (HepG2) tumour cells. It was also more effective than ketoconazole against Penicillium ochrochloron. In turn, the aerial part extract inhibited Salmonella typhimurium more effectively than ampicillin. This study highlights E. viviparum as an unexplored source of bioactive compounds with potential application in the food, pharmaceutical, and other industrial sectors. Consequently, it promotes the interest of conserving this endangered species.
T2  - Industrial Crops and Products
T1  - Phenolic composition and biological activities of the in vitro cultured endangered Eryngium viviparum J. Gay
VL  - 148
DO  - 10.1016/j.indcrop.2020.112325
SP  - 112325
ER  - 

@article{
author = "Ayuso, Manuel and Pinela, José and Dias, Maria Inês and Barros, Lillian and Ivanov, Marija and Calhelha, Ricardo C. and Soković, Marina and Ramil-Rego, Pablo and Barreal, M. Esther and Gallego, Pedro Pablo and Ferreira, Isabel C.F.R.",
year = "2020",
abstract = "Eryngium viviparum is an endangered species that requires management efforts based on complementary ex situ conservation strategies, such as in vitro culture. This study was carried out to evaluate the phenolic composition and the antioxidant, cytotoxic, and antimicrobial properties of E. viviparum aerial and root parts obtained by this micropropagation technique. The HPLC-DAD-ESI/MSn analysis showed that phenolic compounds were more abundant in the root (102 ± 4 mg g−1 extract) than in the aerial part (40.6 ± 0.8 mg g−1 extract) of the plant. The major compound was trans rosmarinic acid, followed by trans 3-O-caffeoylquinic acid. The root extract also showed higher antioxidant activity, with a result close to that of trolox in the thiobarbituric acid reactive substances (TBARS) formation inhibition assay, and a moderate toxicity to lung (NCI-H460), breast (MCF-7) and liver (HepG2) tumour cells. It was also more effective than ketoconazole against Penicillium ochrochloron. In turn, the aerial part extract inhibited Salmonella typhimurium more effectively than ampicillin. This study highlights E. viviparum as an unexplored source of bioactive compounds with potential application in the food, pharmaceutical, and other industrial sectors. Consequently, it promotes the interest of conserving this endangered species.",
journal = "Industrial Crops and Products",
title = "Phenolic composition and biological activities of the in vitro cultured endangered Eryngium viviparum J. Gay",
volume = "148",
doi = "10.1016/j.indcrop.2020.112325",
pages = "112325"
}

Ayuso, M., Pinela, J., Dias, M. I., Barros, L., Ivanov, M., Calhelha, R. C., Soković, M., Ramil-Rego, P., Barreal, M. E., Gallego, P. P.,& Ferreira, I. C.F.R.. (2020). Phenolic composition and biological activities of the in vitro cultured endangered Eryngium viviparum J. Gay. in Industrial Crops and Products, 148, 112325.
https://doi.org/10.1016/j.indcrop.2020.112325

Ayuso M, Pinela J, Dias MI, Barros L, Ivanov M, Calhelha RC, Soković M, Ramil-Rego P, Barreal ME, Gallego PP, Ferreira IC. Phenolic composition and biological activities of the in vitro cultured endangered Eryngium viviparum J. Gay. in Industrial Crops and Products. 2020;148:112325.
doi:10.1016/j.indcrop.2020.112325 .

Ayuso, Manuel, Pinela, José, Dias, Maria Inês, Barros, Lillian, Ivanov, Marija, Calhelha, Ricardo C., Soković, Marina, Ramil-Rego, Pablo, Barreal, M. Esther, Gallego, Pedro Pablo, Ferreira, Isabel C.F.R., "Phenolic composition and biological activities of the in vitro cultured endangered Eryngium viviparum J. Gay" in Industrial Crops and Products, 148 (2020):112325,
https://doi.org/10.1016/j.indcrop.2020.112325 . .