TY  - JOUR
AU  - Garcia-Manieri, Jéssica A.A
AU  - Correa, Vanesa Gesser
AU  - Corrêa, Rúbia Carvalho Gomes
AU  - Dias, Maria Inês
AU  - Calhelha, Ricardo C.
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Barros, Lillian
AU  - Ferreira, Isabel C.F.R.
AU  - Bracht, Adelar
AU  - Peralta, Rosane M.
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S1878818122002572
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5341
AB  - Leaves and barks of Luehea divaricata (Malvaceae) are used in folk medicine for the treatment of infectious diseases, tumors, rheumatism and diabetes. This work presents in vivo experiments (rats and mice) supported by in vitro and chemical analyses in order to address the above mentioned folk medicinal uses. The phenolic constituents of both aqueous and hydroalcoholic extracts are mainly catechin derivatives (135.4 and 215.9 mg/g, respectively). Both extracts displayed strong antioxidant activity. They also showed antiproliferative activities against tumor cells, nitric oxide synthesis inhibition (indicative of anti-inflammatory activity) and antimicrobial activity against a variety of infectious agents. Paracetamol-induced liver injury in rats, which has a high inflammatory component, was substantially attenuated in animals treated with the hydro-alcoholic extract (200 mg kg−1 day−1). This was deduced from aspartate aminotransferase and alanine aminotransferase measurements in plasma as well as from the hepatic activities of catalase and superoxide dismutase. Both extracts inhibited the pancreatic α-amylase with IC50 values of 11.50 ± 0.05 and 18.70 ± 3.87 μg/mL for the hydro-alcoholic and aqueous extract, respectively. Both extracts inhibited starch digestion in mice at the doses of 200 and 400 mg/kg. In general terms the results confirm several of the vox populi notions about the therapeutic potential of L. divaricata, the antidiabetic action, combined with the hepatoprotective effect, being the most relevant observations. Not less important is the economical perspective for the rural producers which may eventually increase their revenue by selling increasingly valued raw materials derived from L. divaricata based on its therapeutic properties.
PB  - Amsterdam: Elsevier
T2  - Biocatalysis and Agricultural Biotechnology
T1  - Polyphenolic profile and pharmacological activities of whips horse (Luehea divaricata) bark extracts studied using in vitro and in vivo systems
VL  - 45
DO  - 10.1016/j.bcab.2022.102530
SP  - 102530
ER  - 

@article{
author = "Garcia-Manieri, Jéssica A.A and Correa, Vanesa Gesser and Corrêa, Rúbia Carvalho Gomes and Dias, Maria Inês and Calhelha, Ricardo C. and Ivanov, Marija and Soković, Marina and Barros, Lillian and Ferreira, Isabel C.F.R. and Bracht, Adelar and Peralta, Rosane M.",
year = "2022",
abstract = "Leaves and barks of Luehea divaricata (Malvaceae) are used in folk medicine for the treatment of infectious diseases, tumors, rheumatism and diabetes. This work presents in vivo experiments (rats and mice) supported by in vitro and chemical analyses in order to address the above mentioned folk medicinal uses. The phenolic constituents of both aqueous and hydroalcoholic extracts are mainly catechin derivatives (135.4 and 215.9 mg/g, respectively). Both extracts displayed strong antioxidant activity. They also showed antiproliferative activities against tumor cells, nitric oxide synthesis inhibition (indicative of anti-inflammatory activity) and antimicrobial activity against a variety of infectious agents. Paracetamol-induced liver injury in rats, which has a high inflammatory component, was substantially attenuated in animals treated with the hydro-alcoholic extract (200 mg kg−1 day−1). This was deduced from aspartate aminotransferase and alanine aminotransferase measurements in plasma as well as from the hepatic activities of catalase and superoxide dismutase. Both extracts inhibited the pancreatic α-amylase with IC50 values of 11.50 ± 0.05 and 18.70 ± 3.87 μg/mL for the hydro-alcoholic and aqueous extract, respectively. Both extracts inhibited starch digestion in mice at the doses of 200 and 400 mg/kg. In general terms the results confirm several of the vox populi notions about the therapeutic potential of L. divaricata, the antidiabetic action, combined with the hepatoprotective effect, being the most relevant observations. Not less important is the economical perspective for the rural producers which may eventually increase their revenue by selling increasingly valued raw materials derived from L. divaricata based on its therapeutic properties.",
publisher = "Amsterdam: Elsevier",
journal = "Biocatalysis and Agricultural Biotechnology",
title = "Polyphenolic profile and pharmacological activities of whips horse (Luehea divaricata) bark extracts studied using in vitro and in vivo systems",
volume = "45",
doi = "10.1016/j.bcab.2022.102530",
pages = "102530"
}

Garcia-Manieri, J. A.A, Correa, V. G., Corrêa, R. C. G., Dias, M. I., Calhelha, R. C., Ivanov, M., Soković, M., Barros, L., Ferreira, I. C.F.R., Bracht, A.,& Peralta, R. M.. (2022). Polyphenolic profile and pharmacological activities of whips horse (Luehea divaricata) bark extracts studied using in vitro and in vivo systems. in Biocatalysis and Agricultural Biotechnology
Amsterdam: Elsevier., 45, 102530.
https://doi.org/10.1016/j.bcab.2022.102530

Garcia-Manieri JA, Correa VG, Corrêa RCG, Dias MI, Calhelha RC, Ivanov M, Soković M, Barros L, Ferreira IC, Bracht A, Peralta RM. Polyphenolic profile and pharmacological activities of whips horse (Luehea divaricata) bark extracts studied using in vitro and in vivo systems. in Biocatalysis and Agricultural Biotechnology. 2022;45:102530.
doi:10.1016/j.bcab.2022.102530 .

Garcia-Manieri, Jéssica A.A, Correa, Vanesa Gesser, Corrêa, Rúbia Carvalho Gomes, Dias, Maria Inês, Calhelha, Ricardo C., Ivanov, Marija, Soković, Marina, Barros, Lillian, Ferreira, Isabel C.F.R., Bracht, Adelar, Peralta, Rosane M., "Polyphenolic profile and pharmacological activities of whips horse (Luehea divaricata) bark extracts studied using in vitro and in vivo systems" in Biocatalysis and Agricultural Biotechnology, 45 (2022):102530,
https://doi.org/10.1016/j.bcab.2022.102530 . .

TY  - JOUR
AU  - Kungel, Pâmela T.A.N.
AU  - Correa, Vanesa G.
AU  - Corrêa, Rúbia C.G.
AU  - Peralta, Rosely Aparecida
AU  - Soković, Marina
AU  - Calhelha, Ricardo C.
AU  - Bracht, Adelar
AU  - Ferreira, Isabel C.F.R.
AU  - Peralta, Rosane M.
PY  - 2018
UR  - https://www.sciencedirect.com/science/article/pii/S0141813018308134?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3044
AB  - This study investigated the antioxidant, antimicrobial and cytotoxic properties of a purified yerba mate polysaccharide. The yerba mate polysaccharide showed a prominent antioxidant activity as evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH)-radical scavenging activity (IC50=1.25±0.10mg/mL), 3-ethyl benzothiazoline-6-sulphonic acid (ABTS+)-radical scavenging activity (IC50=0.41±0.05mg/mL), and hydroxyl scavenging activity (IC50=3.36±0.31mg/mL). The antioxidant activity evaluated as the ferric ion reduction power (FRAP) and oxygen radical absorbance radical assay (ORAC), expressed as trolox equivalents, were 20.84±1.61μMTE/mg and 556.30±12.83μMTE/mg, respectively. The purified yerba mate polysaccharide presented high antimicrobial activity against several bacterial and fungal strains; however, no cytotoxicity against all four tumor human cell lines assessed.
T2  - International Journal of Biological Macromolecules
T1  - Antioxidant and antimicrobial activities of a purified polysaccharide from yerba mate (Ilex paraguariensis)
VL  - 114
DO  - 10.1016/J.IJBIOMAC.2018.04.020
SP  - 1161
EP  - 1167
ER  - 

@article{
author = "Kungel, Pâmela T.A.N. and Correa, Vanesa G. and Corrêa, Rúbia C.G. and Peralta, Rosely Aparecida and Soković, Marina and Calhelha, Ricardo C. and Bracht, Adelar and Ferreira, Isabel C.F.R. and Peralta, Rosane M.",
year = "2018",
abstract = "This study investigated the antioxidant, antimicrobial and cytotoxic properties of a purified yerba mate polysaccharide. The yerba mate polysaccharide showed a prominent antioxidant activity as evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH)-radical scavenging activity (IC50=1.25±0.10mg/mL), 3-ethyl benzothiazoline-6-sulphonic acid (ABTS+)-radical scavenging activity (IC50=0.41±0.05mg/mL), and hydroxyl scavenging activity (IC50=3.36±0.31mg/mL). The antioxidant activity evaluated as the ferric ion reduction power (FRAP) and oxygen radical absorbance radical assay (ORAC), expressed as trolox equivalents, were 20.84±1.61μMTE/mg and 556.30±12.83μMTE/mg, respectively. The purified yerba mate polysaccharide presented high antimicrobial activity against several bacterial and fungal strains; however, no cytotoxicity against all four tumor human cell lines assessed.",
journal = "International Journal of Biological Macromolecules",
title = "Antioxidant and antimicrobial activities of a purified polysaccharide from yerba mate (Ilex paraguariensis)",
volume = "114",
doi = "10.1016/J.IJBIOMAC.2018.04.020",
pages = "1161-1167"
}

Kungel, P. T.A.N., Correa, V. G., Corrêa, R. C.G., Peralta, R. A., Soković, M., Calhelha, R. C., Bracht, A., Ferreira, I. C.F.R.,& Peralta, R. M.. (2018). Antioxidant and antimicrobial activities of a purified polysaccharide from yerba mate (Ilex paraguariensis). in International Journal of Biological Macromolecules, 114, 1161-1167.
https://doi.org/10.1016/J.IJBIOMAC.2018.04.020

Kungel PT, Correa VG, Corrêa RC, Peralta RA, Soković M, Calhelha RC, Bracht A, Ferreira IC, Peralta RM. Antioxidant and antimicrobial activities of a purified polysaccharide from yerba mate (Ilex paraguariensis). in International Journal of Biological Macromolecules. 2018;114:1161-1167.
doi:10.1016/J.IJBIOMAC.2018.04.020 .

Kungel, Pâmela T.A.N., Correa, Vanesa G., Corrêa, Rúbia C.G., Peralta, Rosely Aparecida, Soković, Marina, Calhelha, Ricardo C., Bracht, Adelar, Ferreira, Isabel C.F.R., Peralta, Rosane M., "Antioxidant and antimicrobial activities of a purified polysaccharide from yerba mate (Ilex paraguariensis)" in International Journal of Biological Macromolecules, 114 (2018):1161-1167,
https://doi.org/10.1016/J.IJBIOMAC.2018.04.020 . .

TY  - JOUR
AU  - Corrêa, Rúbia C. G.
AU  - Barros, Lillian
AU  - Fernandes, Ângela
AU  - Soković, Marina
AU  - Bracht, Adelar
AU  - Peralta, Rosane M.
AU  - Ferreira, Isabel C. F. R.
PY  - 2018
UR  - http://xlink.rsc.org/?DOI=C7FO02007D
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3028
AB  - In recent years, mycosterols have emerged as potential functional ingredients for the development of sterol-enriched food products and dietary supplements. Agaricus blazei is a mushroom rich in bioactive compounds. For commercial purposes, their fruiting bodies must obey rigid morphological criteria. Those not conforming to these criteria are usually discarded, although this does not mean impairment of their content in bioactives. The aim of the present work was to propose the use of commercially discarded A. blazei fruiting bodies for obtaining an extract rich in ergosterol as a fortifier ingredient for yogurts. For extraction, the Soxhlet technology was used and the highest ergosterol yield (around 12%) was achieved in the 5thcycle, yielding 58.53 ± 1.72 µg of ergosterol per 100 g of mushroom (dry weight). The ergosterol rich extract presented notable antioxidant and antimicrobial properties, besides showing no hepatotoxicity. When added to the yogurts it significantly enhanced their antioxidant properties. Furthermore, it did not significantly alter the nutritional or the individual fatty acid profiles of the final dairy products. Thus, A. blazei fruiting bodies that do not conform to the commercial requirements of the market and are normally discarded could be exploited for obtaining a natural high added-value food additive, following the circular bioeconomy concept.
T2  - Food and Function
T1  - A natural food ingredient based on ergosterol: optimization of the extraction from Agaricus blazei, evaluation of bioactive properties and incorporation in yogurts.
IS  - 3
VL  - 9
DO  - 10.1039/c7fo02007d
SP  - 1465
EP  - 1474
ER  - 

@article{
author = "Corrêa, Rúbia C. G. and Barros, Lillian and Fernandes, Ângela and Soković, Marina and Bracht, Adelar and Peralta, Rosane M. and Ferreira, Isabel C. F. R.",
year = "2018",
abstract = "In recent years, mycosterols have emerged as potential functional ingredients for the development of sterol-enriched food products and dietary supplements. Agaricus blazei is a mushroom rich in bioactive compounds. For commercial purposes, their fruiting bodies must obey rigid morphological criteria. Those not conforming to these criteria are usually discarded, although this does not mean impairment of their content in bioactives. The aim of the present work was to propose the use of commercially discarded A. blazei fruiting bodies for obtaining an extract rich in ergosterol as a fortifier ingredient for yogurts. For extraction, the Soxhlet technology was used and the highest ergosterol yield (around 12%) was achieved in the 5thcycle, yielding 58.53 ± 1.72 µg of ergosterol per 100 g of mushroom (dry weight). The ergosterol rich extract presented notable antioxidant and antimicrobial properties, besides showing no hepatotoxicity. When added to the yogurts it significantly enhanced their antioxidant properties. Furthermore, it did not significantly alter the nutritional or the individual fatty acid profiles of the final dairy products. Thus, A. blazei fruiting bodies that do not conform to the commercial requirements of the market and are normally discarded could be exploited for obtaining a natural high added-value food additive, following the circular bioeconomy concept.",
journal = "Food and Function",
title = "A natural food ingredient based on ergosterol: optimization of the extraction from Agaricus blazei, evaluation of bioactive properties and incorporation in yogurts.",
number = "3",
volume = "9",
doi = "10.1039/c7fo02007d",
pages = "1465-1474"
}

Corrêa, R. C. G., Barros, L., Fernandes, Â., Soković, M., Bracht, A., Peralta, R. M.,& Ferreira, I. C. F. R.. (2018). A natural food ingredient based on ergosterol: optimization of the extraction from Agaricus blazei, evaluation of bioactive properties and incorporation in yogurts.. in Food and Function, 9(3), 1465-1474.
https://doi.org/10.1039/c7fo02007d

Corrêa RCG, Barros L, Fernandes Â, Soković M, Bracht A, Peralta RM, Ferreira ICFR. A natural food ingredient based on ergosterol: optimization of the extraction from Agaricus blazei, evaluation of bioactive properties and incorporation in yogurts.. in Food and Function. 2018;9(3):1465-1474.
doi:10.1039/c7fo02007d .

Corrêa, Rúbia C. G., Barros, Lillian, Fernandes, Ângela, Soković, Marina, Bracht, Adelar, Peralta, Rosane M., Ferreira, Isabel C. F. R., "A natural food ingredient based on ergosterol: optimization of the extraction from Agaricus blazei, evaluation of bioactive properties and incorporation in yogurts." in Food and Function, 9, no. 3 (2018):1465-1474,
https://doi.org/10.1039/c7fo02007d . .