TY  - JOUR
AU  - Nešić, Dejan
AU  - Stevanović, Darko M.
AU  - Ajdžanović, Vladimir
AU  - Velkovski, Saško D.
AU  - Milošević, Verica
AU  - Starčević, Vesna P.
PY  - 2005
PY  - 2005
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/156
AB  - Arginine vasopressin (AVP) is synthesized in specific brain regions including the magnocellular and parvocellular divisions of paraventricular nucleus (PVN). While magnocellular AVP responds to osmotic stimuli and functions mainly - although not exclusively - as an antidiuretic hormone, AVP produced in the parvocellular region controls the hypothalamus-pituitary-adrenal (HPA) axis, in conjunction with CRH (Ferrini et al., 1997). ACTH release from anterior pituitary gland is principally driven by two hypothalamic hormones, corticotropin-releasing hormone (CRH) and arginine-vasopressin (AVP) (Tsigos and Chrousos, 2002).
T2  - Acta biologica iugoslavica - serija C: Physiologica et pharmacologica acta
T1  - Efekti centralno aplikovanog vazopresina na hipofizne ACTH ćelije kod mužjaka pacova
T1  - Effects of centrally applied vasopressin on the pituitary ACTH cells in male rats
IS  - 2
VL  - 41
SP  - 79
EP  - 82
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_156
ER  - 

@article{
author = "Nešić, Dejan and Stevanović, Darko M. and Ajdžanović, Vladimir and Velkovski, Saško D. and Milošević, Verica and Starčević, Vesna P.",
year = "2005, 2005",
abstract = "Arginine vasopressin (AVP) is synthesized in specific brain regions including the magnocellular and parvocellular divisions of paraventricular nucleus (PVN). While magnocellular AVP responds to osmotic stimuli and functions mainly - although not exclusively - as an antidiuretic hormone, AVP produced in the parvocellular region controls the hypothalamus-pituitary-adrenal (HPA) axis, in conjunction with CRH (Ferrini et al., 1997). ACTH release from anterior pituitary gland is principally driven by two hypothalamic hormones, corticotropin-releasing hormone (CRH) and arginine-vasopressin (AVP) (Tsigos and Chrousos, 2002).",
journal = "Acta biologica iugoslavica - serija C: Physiologica et pharmacologica acta",
title = "Efekti centralno aplikovanog vazopresina na hipofizne ACTH ćelije kod mužjaka pacova, Effects of centrally applied vasopressin on the pituitary ACTH cells in male rats",
number = "2",
volume = "41",
pages = "79-82",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_156"
}

Nešić, D., Stevanović, D. M., Ajdžanović, V., Velkovski, S. D., Milošević, V.,& Starčević, V. P.. (2005). Efekti centralno aplikovanog vazopresina na hipofizne ACTH ćelije kod mužjaka pacova. in Acta biologica iugoslavica - serija C: Physiologica et pharmacologica acta, 41(2), 79-82.
https://hdl.handle.net/21.15107/rcub_ibiss_156

Nešić D, Stevanović DM, Ajdžanović V, Velkovski SD, Milošević V, Starčević VP. Efekti centralno aplikovanog vazopresina na hipofizne ACTH ćelije kod mužjaka pacova. in Acta biologica iugoslavica - serija C: Physiologica et pharmacologica acta. 2005;41(2):79-82.
https://hdl.handle.net/21.15107/rcub_ibiss_156 .

Nešić, Dejan, Stevanović, Darko M., Ajdžanović, Vladimir, Velkovski, Saško D., Milošević, Verica, Starčević, Vesna P., "Efekti centralno aplikovanog vazopresina na hipofizne ACTH ćelije kod mužjaka pacova" in Acta biologica iugoslavica - serija C: Physiologica et pharmacologica acta, 41, no. 2 (2005):79-82,
https://hdl.handle.net/21.15107/rcub_ibiss_156 .

TY  - JOUR
AU  - Milošević, Verica
AU  - Manojlović-Stojanoski, Milica
AU  - Kostić, Nada
AU  - Brkić, Branislava
AU  - Velkovski, Saško D.
AU  - Šošić-Jurjević, Branka 
AU  - Trifunović, Svetlana
AU  - Starčević, Vesna P.
PY  - 2003
PY  - 2003
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/67
AB  - The results on the effects of synthetic somatostatins on the secretion of ACTH have been rather conflicting, but some authors reported that somatostain is involved in the regulation of ACTH secretion in rats of both sexes. The inhibitory mechanism of ACTH secretion could operate through the inhibition of CRF release from the hypothalamus. In the present study, we have investigated the effects of Octreotide, a somatostain analogue given intracerebroventricularly (i.c.v) in low doses, on morphometric characteristics of pituitary adrenocorticotropes (ACTH cells) in adult Wistar rat males. The animals were i.c.v. given three 1.0 µg doses of Octreotide dissolved in 10 µL saline every second day. The controls were treated in the same way with the same volume of saline. ACTH-producing cells were studied using the peroxidase-antiperoxidase (PAP) immunohistochemical procedure. Body weight, as well as absolute and relative pituitary weights were not significantly changed in comparison with the corresponding controls (p>0.05). The characteristics of immunohistochemicaly labelled ACTH cells in intact rat males were as follows: localization between the capillaries stellate in shape with the cytoplasmatic processes among neighbouring cells. The nucleus follows the shape of the cell body. Small specific secretory granules were distributed mainly at the periphery of the cytoplasm. The shape, and localization of ACTH immunoreactive cells in Octreotide-treated animals were not significantly changed in comparison with the controls. Octreotide treatment significantly decreased all morphometric parameters measured, i.e. volume of ACTH cells and their nuclei by 44% and 18% respectively, as compared to the controls (p<0.05). Volume densities were also significantly decreased (by 31%; p<0.05) comparing to the corresponding controls. These results indicate that Octreotide applied i.c.v. exerts a significant inhibitory effect on the immunohistochemical and morphometric characteristics of ACTH cells in rat males.
AB  - Postoje veoma različita, a često i suprotstavljena mišljenja o efektima sintetskih somatostatina na lučenje adrenokortikotropnog hormona (ACTH) iz hipofize. Mogući mehanizam inhibicije lučenja ACTH je preko inhibicije lučenja CRF iz hipotalamusa. U ovom radu su ispitivani efekti intracerebroventrikularno (i.c.v) ubrizganog analoga somatostatina Oktreotida, na rast ACTH ćelija adenohipofize mužjaka Wistar pacova. Sve eksperimentalne životinje su primile tri doze od po 1.0 µg Oktreotida rastvorenog u 10 µL fiziološkog rastvora, svakog drugog dana. Kontrole su tretirane na isti način fiziološkim rastvorom. Pacovi su žrtvovani pet dana posle poslednje injekcije. ACTH ćelije su imunocitohemijski bojene metodom peroksidaza-antiperoksidaza (PAP). Telesna masa, apsolutna i relativna masa hipofize nisu bile statistički značajno promenjene u poređenju sa odgovarajućom kontrolom (p>0.05). Karakteristike imunohistohemijski obojenih ACTH ćelija kontrolnih pacova su: lokalizacija između kapilara, zvezdast oblik sa citoplazmatičnim produžecima između drugih ćelija. Male specifične sekretorne granule raspoređene su po periferiji citoplazme. Oblik i lokalizacija ACTH ćelija životinja tretiranih Oktreotidom nisu bili značajno promenjeni u odnosu na kontrolu. Međutim, oktreotid je izazvao značajno smanjenje svih morfometrijskih parametara ovih ćelija (p>0.05). Zapremina ćelija je bila smanjena za 44%, jedara za 18%, a volumenska gustina za 31% (p<0.05) u poređenju sa odgovarajućom kontrolom. Na osnovu dobijenih rezultata može se zaključiti za Oktreotid deluje inhibitorno na rast ACTH ćelija adenohipofize mužjaka pacova.
PB  - Unija bioloških naučnih društava Jugoslavije
T2  - Acta biologica iugoslavica - serija C: Physiologica et pharmacologica acta
T1  - Efekti oktreotida na acth ćelije hipofize mužjaka pacova
T1  - A somatostatin analogue octreotide inhibits pituitary ACHT cells in rat males
IS  - 1
VL  - 39
SP  - 25
EP  - 30
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_67
ER  - 

@article{
author = "Milošević, Verica and Manojlović-Stojanoski, Milica and Kostić, Nada and Brkić, Branislava and Velkovski, Saško D. and Šošić-Jurjević, Branka  and Trifunović, Svetlana and Starčević, Vesna P.",
year = "2003, 2003",
abstract = "The results on the effects of synthetic somatostatins on the secretion of ACTH have been rather conflicting, but some authors reported that somatostain is involved in the regulation of ACTH secretion in rats of both sexes. The inhibitory mechanism of ACTH secretion could operate through the inhibition of CRF release from the hypothalamus. In the present study, we have investigated the effects of Octreotide, a somatostain analogue given intracerebroventricularly (i.c.v) in low doses, on morphometric characteristics of pituitary adrenocorticotropes (ACTH cells) in adult Wistar rat males. The animals were i.c.v. given three 1.0 µg doses of Octreotide dissolved in 10 µL saline every second day. The controls were treated in the same way with the same volume of saline. ACTH-producing cells were studied using the peroxidase-antiperoxidase (PAP) immunohistochemical procedure. Body weight, as well as absolute and relative pituitary weights were not significantly changed in comparison with the corresponding controls (p>0.05). The characteristics of immunohistochemicaly labelled ACTH cells in intact rat males were as follows: localization between the capillaries stellate in shape with the cytoplasmatic processes among neighbouring cells. The nucleus follows the shape of the cell body. Small specific secretory granules were distributed mainly at the periphery of the cytoplasm. The shape, and localization of ACTH immunoreactive cells in Octreotide-treated animals were not significantly changed in comparison with the controls. Octreotide treatment significantly decreased all morphometric parameters measured, i.e. volume of ACTH cells and their nuclei by 44% and 18% respectively, as compared to the controls (p<0.05). Volume densities were also significantly decreased (by 31%; p<0.05) comparing to the corresponding controls. These results indicate that Octreotide applied i.c.v. exerts a significant inhibitory effect on the immunohistochemical and morphometric characteristics of ACTH cells in rat males., Postoje veoma različita, a često i suprotstavljena mišljenja o efektima sintetskih somatostatina na lučenje adrenokortikotropnog hormona (ACTH) iz hipofize. Mogući mehanizam inhibicije lučenja ACTH je preko inhibicije lučenja CRF iz hipotalamusa. U ovom radu su ispitivani efekti intracerebroventrikularno (i.c.v) ubrizganog analoga somatostatina Oktreotida, na rast ACTH ćelija adenohipofize mužjaka Wistar pacova. Sve eksperimentalne životinje su primile tri doze od po 1.0 µg Oktreotida rastvorenog u 10 µL fiziološkog rastvora, svakog drugog dana. Kontrole su tretirane na isti način fiziološkim rastvorom. Pacovi su žrtvovani pet dana posle poslednje injekcije. ACTH ćelije su imunocitohemijski bojene metodom peroksidaza-antiperoksidaza (PAP). Telesna masa, apsolutna i relativna masa hipofize nisu bile statistički značajno promenjene u poređenju sa odgovarajućom kontrolom (p>0.05). Karakteristike imunohistohemijski obojenih ACTH ćelija kontrolnih pacova su: lokalizacija između kapilara, zvezdast oblik sa citoplazmatičnim produžecima između drugih ćelija. Male specifične sekretorne granule raspoređene su po periferiji citoplazme. Oblik i lokalizacija ACTH ćelija životinja tretiranih Oktreotidom nisu bili značajno promenjeni u odnosu na kontrolu. Međutim, oktreotid je izazvao značajno smanjenje svih morfometrijskih parametara ovih ćelija (p>0.05). Zapremina ćelija je bila smanjena za 44%, jedara za 18%, a volumenska gustina za 31% (p<0.05) u poređenju sa odgovarajućom kontrolom. Na osnovu dobijenih rezultata može se zaključiti za Oktreotid deluje inhibitorno na rast ACTH ćelija adenohipofize mužjaka pacova.",
publisher = "Unija bioloških naučnih društava Jugoslavije",
journal = "Acta biologica iugoslavica - serija C: Physiologica et pharmacologica acta",
title = "Efekti oktreotida na acth ćelije hipofize mužjaka pacova, A somatostatin analogue octreotide inhibits pituitary ACHT cells in rat males",
number = "1",
volume = "39",
pages = "25-30",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_67"
}

Milošević, V., Manojlović-Stojanoski, M., Kostić, N., Brkić, B., Velkovski, S. D., Šošić-Jurjević, B., Trifunović, S.,& Starčević, V. P.. (2003). Efekti oktreotida na acth ćelije hipofize mužjaka pacova. in Acta biologica iugoslavica - serija C: Physiologica et pharmacologica acta
Unija bioloških naučnih društava Jugoslavije., 39(1), 25-30.
https://hdl.handle.net/21.15107/rcub_ibiss_67

Milošević V, Manojlović-Stojanoski M, Kostić N, Brkić B, Velkovski SD, Šošić-Jurjević B, Trifunović S, Starčević VP. Efekti oktreotida na acth ćelije hipofize mužjaka pacova. in Acta biologica iugoslavica - serija C: Physiologica et pharmacologica acta. 2003;39(1):25-30.
https://hdl.handle.net/21.15107/rcub_ibiss_67 .

Milošević, Verica, Manojlović-Stojanoski, Milica, Kostić, Nada, Brkić, Branislava, Velkovski, Saško D., Šošić-Jurjević, Branka , Trifunović, Svetlana, Starčević, Vesna P., "Efekti oktreotida na acth ćelije hipofize mužjaka pacova" in Acta biologica iugoslavica - serija C: Physiologica et pharmacologica acta, 39, no. 1 (2003):25-30,
https://hdl.handle.net/21.15107/rcub_ibiss_67 .

TY  - CONF
AU  - Velkovski, Saško
AU  - Ristić, Snežana
AU  - Milošević, Verica
AU  - Saičić, Zorica
AU  - Spasić, Mihajlo
AU  - Pavlović, Slađan
AU  - Nikolić, Aleksandra
AU  - Blagojević, Duško
AU  - Starčević, Vesna
PY  - 2002
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5958
AB  - Postoji veli ki broj podataka o pokusajima uspostavljanja pouzdanog animalnog modela atcroskleroze, ali jos n ije poznat  dovoljno  dobar  analog  humanoj  aterosk lerozi. Kao prilog ovim is trazivanjima, pokusali smo da proce­ nimo doprinos  reaktivnih  vrsta  kiseonika  (ROS)  u  ranoj ate rogenezi i  prevcntivnu  ulogu  cndogenog  antioksida ­ cio nog zastitnog sis tema (AOS). Odrasli muzjaci Wistar albin o pacova, te les ne masc  oko  400  g,  korisceni  su  za sp rovodenje specijalne ate rogenc dijctc  koja se sas toja la od  smcse  masti  ( buter,  holesterol)  i  drugih   sup stanc i ( ho ln a kise li na, holi n hlorid, tiourac i l ). Kontrolnc i eks­ per imenta lne zivo tin je su bi le srnestcne u plasticne  ka­ veze  i  imale  s u  dostu pne  peleti ranu   hranu  i  vodu po volji. Eksperimentalne zivotinje su dobijale svakoga dana u toku 8 nedeija po 5 g aterogene smese, sondom, intragastricno. Na  kraju  dijete  sve zivotinje  su zrtvovane i sakupljena je krv za odredivanje (1) lipidnog statusa: koncentracija triglicerida (TG), ukupnog holesterola (CH). LDL holesterola i HDL holesterola u plazmi, od­ nosa  koncentracija  LDL i HDL  holesterola  (LDL/HDL) i (2) antioksidacionog statusa u krvi: aktivnost kata­ laze u eritrocitima (Er CAT), aktivnost glutation-S-tran­ sferaze (GST) u  plazmi,  koncentracija  glutationa  (GSH) u plazmi i koncentracija vitamina E (Vit. E) u plazmi. Uzimani su isecci abdominalne aorte za histolosku veri­ fikaciju ateroskle roze. Razlike izmedu vrednosti kon­ trolnih i eksperimentalnih zivotinja testirane su  stati­ sticki.
Analiza hitoloskih praparata aorte pokazala je normalnu gradu arterijskog zida u kontrolnih zivotinja , dok su u eksperimentalnih zivotinja nadene inicijalne promene u intimi (zadebljanje i  celijska akumulacija). Vrednosti svih pracenih varijabli lipidnog i  antioksidacionog  sta­ tusa u kontrolnih zivotinja bile su normalne. U eksperi­ inentalnih zivotinja postojalo je statisticki znacajno po­ vecanje koncentracije TG  (p < 0,05),  koncentracije  CH (p < 0,01), koncentracije LDL (p < 0,01) i odnosa LDL/HDL (p < 0,01) u poredenju sa kontrolnim zivoti­ njama . Koncentracija HDL bila je nepromenjena (p > 0,05). Aktivnost Er CAT i koncentracija  GSH  u plazmi bile su povecane  u  eksperimentalnih  zivotinja  u odnosu na kontrolne,  ali  ne znacajno (p > 0,05). Aktivnost  GST  u plazmi nije bila promenjena (p > 0,05). Samo kon­ centracija Vit. E u  plazmi eksperimentalnih  zivotinja bila je statisticki znacajno veca  u poredenju  sa  kontrol­ nim zivotinjama (p < 0,01). Mozemo zakljuciti da anti­ oksidacioni status u krvi nije bio u korelaciji sa lipidnim statusom  u toku rane aterogeneze  u odraslih  pacova.
PB  - Priština: Academy of Sciences and Arts of SAP Kosovo. Division of Natural Sciences: Society for Biology and Experimental Medicine SAP Kosovo
C3  - Conference of the Serbian Physiological Society; 2001 Dec 20-23; Zlatibor, Serbia
T1  - Lipidni i antioksidacioni status u animalnom modelu eksperimentalne ateroskleroze
SP  - 64
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5958
ER  - 

@conference{
author = "Velkovski, Saško and Ristić, Snežana and Milošević, Verica and Saičić, Zorica and Spasić, Mihajlo and Pavlović, Slađan and Nikolić, Aleksandra and Blagojević, Duško and Starčević, Vesna",
year = "2002",
abstract = "Postoji veli ki broj podataka o pokusajima uspostavljanja pouzdanog animalnog modela atcroskleroze, ali jos n ije poznat  dovoljno  dobar  analog  humanoj  aterosk lerozi. Kao prilog ovim is trazivanjima, pokusali smo da proce­ nimo doprinos  reaktivnih  vrsta  kiseonika  (ROS)  u  ranoj ate rogenezi i  prevcntivnu  ulogu  cndogenog  antioksida ­ cio nog zastitnog sis tema (AOS). Odrasli muzjaci Wistar albin o pacova, te les ne masc  oko  400  g,  korisceni  su  za sp rovodenje specijalne ate rogenc dijctc  koja se sas toja la od  smcse  masti  ( buter,  holesterol)  i  drugih   sup stanc i ( ho ln a kise li na, holi n hlorid, tiourac i l ). Kontrolnc i eks­ per imenta lne zivo tin je su bi le srnestcne u plasticne  ka­ veze  i  imale  s u  dostu pne  peleti ranu   hranu  i  vodu po volji. Eksperimentalne zivotinje su dobijale svakoga dana u toku 8 nedeija po 5 g aterogene smese, sondom, intragastricno. Na  kraju  dijete  sve zivotinje  su zrtvovane i sakupljena je krv za odredivanje (1) lipidnog statusa: koncentracija triglicerida (TG), ukupnog holesterola (CH). LDL holesterola i HDL holesterola u plazmi, od­ nosa  koncentracija  LDL i HDL  holesterola  (LDL/HDL) i (2) antioksidacionog statusa u krvi: aktivnost kata­ laze u eritrocitima (Er CAT), aktivnost glutation-S-tran­ sferaze (GST) u  plazmi,  koncentracija  glutationa  (GSH) u plazmi i koncentracija vitamina E (Vit. E) u plazmi. Uzimani su isecci abdominalne aorte za histolosku veri­ fikaciju ateroskle roze. Razlike izmedu vrednosti kon­ trolnih i eksperimentalnih zivotinja testirane su  stati­ sticki.
Analiza hitoloskih praparata aorte pokazala je normalnu gradu arterijskog zida u kontrolnih zivotinja , dok su u eksperimentalnih zivotinja nadene inicijalne promene u intimi (zadebljanje i  celijska akumulacija). Vrednosti svih pracenih varijabli lipidnog i  antioksidacionog  sta­ tusa u kontrolnih zivotinja bile su normalne. U eksperi­ inentalnih zivotinja postojalo je statisticki znacajno po­ vecanje koncentracije TG  (p < 0,05),  koncentracije  CH (p < 0,01), koncentracije LDL (p < 0,01) i odnosa LDL/HDL (p < 0,01) u poredenju sa kontrolnim zivoti­ njama . Koncentracija HDL bila je nepromenjena (p > 0,05). Aktivnost Er CAT i koncentracija  GSH  u plazmi bile su povecane  u  eksperimentalnih  zivotinja  u odnosu na kontrolne,  ali  ne znacajno (p > 0,05). Aktivnost  GST  u plazmi nije bila promenjena (p > 0,05). Samo kon­ centracija Vit. E u  plazmi eksperimentalnih  zivotinja bila je statisticki znacajno veca  u poredenju  sa  kontrol­ nim zivotinjama (p < 0,01). Mozemo zakljuciti da anti­ oksidacioni status u krvi nije bio u korelaciji sa lipidnim statusom  u toku rane aterogeneze  u odraslih  pacova.",
publisher = "Priština: Academy of Sciences and Arts of SAP Kosovo. Division of Natural Sciences: Society for Biology and Experimental Medicine SAP Kosovo",
journal = "Conference of the Serbian Physiological Society; 2001 Dec 20-23; Zlatibor, Serbia",
title = "Lipidni i antioksidacioni status u animalnom modelu eksperimentalne ateroskleroze",
pages = "64",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5958"
}

Velkovski, S., Ristić, S., Milošević, V., Saičić, Z., Spasić, M., Pavlović, S., Nikolić, A., Blagojević, D.,& Starčević, V.. (2002). Lipidni i antioksidacioni status u animalnom modelu eksperimentalne ateroskleroze. in Conference of the Serbian Physiological Society; 2001 Dec 20-23; Zlatibor, Serbia
Priština: Academy of Sciences and Arts of SAP Kosovo. Division of Natural Sciences: Society for Biology and Experimental Medicine SAP Kosovo., 64.
https://hdl.handle.net/21.15107/rcub_ibiss_5958

Velkovski S, Ristić S, Milošević V, Saičić Z, Spasić M, Pavlović S, Nikolić A, Blagojević D, Starčević V. Lipidni i antioksidacioni status u animalnom modelu eksperimentalne ateroskleroze. in Conference of the Serbian Physiological Society; 2001 Dec 20-23; Zlatibor, Serbia. 2002;:64.
https://hdl.handle.net/21.15107/rcub_ibiss_5958 .

Velkovski, Saško, Ristić, Snežana, Milošević, Verica, Saičić, Zorica, Spasić, Mihajlo, Pavlović, Slađan, Nikolić, Aleksandra, Blagojević, Duško, Starčević, Vesna, "Lipidni i antioksidacioni status u animalnom modelu eksperimentalne ateroskleroze" in Conference of the Serbian Physiological Society; 2001 Dec 20-23; Zlatibor, Serbia (2002):64,
https://hdl.handle.net/21.15107/rcub_ibiss_5958 .

TY  - JOUR
AU  - Velkovski, Saško D.
AU  - Mazić, Sanja
AU  - Nešić, Dejan M.
AU  - Igrački, Iva
AU  - Milošević, Verica
AU  - Starčević, Vesna P.
PY  - 2002
PY  - 2002
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/460
AB  - In atherosclerosis researches different animal models are used but the most common is the rabbit, because of the easy development of atherosclerotic lesions. Atherosclerosis is a multicellular process and platelets play an important role in atherogenesis. Excessive plasma lipids stimulate platelet aggregability and thus atherosclerosis development. The effects of an atherogenic diet on lipid status, abdominal aorta wall structure, and platelet aggregability were studied in rabbits. Adult male Chinchilla rabbits were fed an atherogenic diet (2% edible oil solution of crystaline cholesterol at 8 mg cholesterol per kg b.wt.daily) for 8 weeks. Plasma lipid levels (triglycerides, total cholesterol, LDL-cholesterol, HDL-cholesterol and LDL/HDL ratio) and platelet aggregability were then measured and compared to the values obtained for control animals. Histological analysis showed atherogenic changes in the abdominal aorta wall of the experimental animals. Total plasma cholesterol level, plasma LDL-cholesterol, and LDL/HDL ratio were significantly increased compared to the controls (p<0.01), as well as triglyceride and HDL-cholesterol levels (p <0.05). Platelet aggregability was also significantly increased (p<0.01) after the atherogenic diet in comparison with controls when ADP, at concentrations both of 5 and 10 (mol/L, was used as the aggregant. Disaggregation was common in control animals but this phenomenon was not recorded in the experimental animals. It can be concluded that an 8-week atherogenic diet in rabbits induced marked changes of the lipid parameters, provoked atherogenic changes in the abdominal aorta wall and increased platelet aggregability.
AB  - U istraživanjima ateroskleroze korišćeni su razlčiti animalni modeli, ali najčešće korišćene životinje su kunići zbog toga što lako razvijaju aterosklerozne lezije. Ateroskleroza je multicelularni proces i trombociti igraju važnu ulogu u aterogenezi. Prekomerna koncentracija lipida u plazmi izaziva povećanu agregaciju trombocita i na taj način pospešuje razvoj ateroskleroze. Istraživali smo efekte aterogene dijete na lipidni status promene na" abdominalnoj aorti i agregabilnost trombocita u kunića. Odrasli mužjaci Cinčila kunića bill su podvrgnuti aterogenoj dijeti koja se sastojala od 2% rastvora kristalnog holesterola u jestivom ulju u toku 8 nedelja. Dnevna doza holesterola bila je 8 mg/kg telesne mase. Po završetku dijete, određivane su koncentracije triglicerida, ukupnog holesterola, LDL holesterola i HDL holesterola, odnos LDL/HDL, kao i agregabilnost trombocita. Vrednosti su poređene sa kontrolnim životinjama. Histološka analiza je pokazala aterogene promene u zidu abdominalne aorte eksperimentalnih životinja. Koncentracije ukupnog i LDL holesterola u plazmi kao i odnos LDL/HDL bili su visoko statisticki značajno povećane (p<0,01). Koncentracije triglicerida i HDL-holesterola u plazmi takođe su bile statističk značajno povećane (p<0,05). Agregabilnost trombocita bila je statisticki visoko značajno povećana (p<0,01) posle aterogene dijete, bez obzira na to da II je agregant ADP korišćen u koncentraciji 5 ili 10 (mol/L. Fenomen dezagregacije bio je uobičajen za kontrolne životinje, ali nije registrovan kod eksperimentalnih životinja. Može se zaključiti da je osmonedeljna aterogena dijeta u kunića izazvala značajne promene lipidnih varijabli, izazvala nastanak aterogenih promena u zidu abdominalne aorte i povećala agregabilnost trombocita.
T2  - Acta veterinaria
T1  - Povećana agregabilnost trombocita posle aterogene dijete u kunića
T1  - Increased platelet aggregability following an atherogenic diet in rabbits
IS  - 1
VL  - 52
SP  - 11
EP  - 18
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_460
ER  - 

@article{
author = "Velkovski, Saško D. and Mazić, Sanja and Nešić, Dejan M. and Igrački, Iva and Milošević, Verica and Starčević, Vesna P.",
year = "2002, 2002",
abstract = "In atherosclerosis researches different animal models are used but the most common is the rabbit, because of the easy development of atherosclerotic lesions. Atherosclerosis is a multicellular process and platelets play an important role in atherogenesis. Excessive plasma lipids stimulate platelet aggregability and thus atherosclerosis development. The effects of an atherogenic diet on lipid status, abdominal aorta wall structure, and platelet aggregability were studied in rabbits. Adult male Chinchilla rabbits were fed an atherogenic diet (2% edible oil solution of crystaline cholesterol at 8 mg cholesterol per kg b.wt.daily) for 8 weeks. Plasma lipid levels (triglycerides, total cholesterol, LDL-cholesterol, HDL-cholesterol and LDL/HDL ratio) and platelet aggregability were then measured and compared to the values obtained for control animals. Histological analysis showed atherogenic changes in the abdominal aorta wall of the experimental animals. Total plasma cholesterol level, plasma LDL-cholesterol, and LDL/HDL ratio were significantly increased compared to the controls (p<0.01), as well as triglyceride and HDL-cholesterol levels (p <0.05). Platelet aggregability was also significantly increased (p<0.01) after the atherogenic diet in comparison with controls when ADP, at concentrations both of 5 and 10 (mol/L, was used as the aggregant. Disaggregation was common in control animals but this phenomenon was not recorded in the experimental animals. It can be concluded that an 8-week atherogenic diet in rabbits induced marked changes of the lipid parameters, provoked atherogenic changes in the abdominal aorta wall and increased platelet aggregability., U istraživanjima ateroskleroze korišćeni su razlčiti animalni modeli, ali najčešće korišćene životinje su kunići zbog toga što lako razvijaju aterosklerozne lezije. Ateroskleroza je multicelularni proces i trombociti igraju važnu ulogu u aterogenezi. Prekomerna koncentracija lipida u plazmi izaziva povećanu agregaciju trombocita i na taj način pospešuje razvoj ateroskleroze. Istraživali smo efekte aterogene dijete na lipidni status promene na" abdominalnoj aorti i agregabilnost trombocita u kunića. Odrasli mužjaci Cinčila kunića bill su podvrgnuti aterogenoj dijeti koja se sastojala od 2% rastvora kristalnog holesterola u jestivom ulju u toku 8 nedelja. Dnevna doza holesterola bila je 8 mg/kg telesne mase. Po završetku dijete, određivane su koncentracije triglicerida, ukupnog holesterola, LDL holesterola i HDL holesterola, odnos LDL/HDL, kao i agregabilnost trombocita. Vrednosti su poređene sa kontrolnim životinjama. Histološka analiza je pokazala aterogene promene u zidu abdominalne aorte eksperimentalnih životinja. Koncentracije ukupnog i LDL holesterola u plazmi kao i odnos LDL/HDL bili su visoko statisticki značajno povećane (p<0,01). Koncentracije triglicerida i HDL-holesterola u plazmi takođe su bile statističk značajno povećane (p<0,05). Agregabilnost trombocita bila je statisticki visoko značajno povećana (p<0,01) posle aterogene dijete, bez obzira na to da II je agregant ADP korišćen u koncentraciji 5 ili 10 (mol/L. Fenomen dezagregacije bio je uobičajen za kontrolne životinje, ali nije registrovan kod eksperimentalnih životinja. Može se zaključiti da je osmonedeljna aterogena dijeta u kunića izazvala značajne promene lipidnih varijabli, izazvala nastanak aterogenih promena u zidu abdominalne aorte i povećala agregabilnost trombocita.",
journal = "Acta veterinaria",
title = "Povećana agregabilnost trombocita posle aterogene dijete u kunića, Increased platelet aggregability following an atherogenic diet in rabbits",
number = "1",
volume = "52",
pages = "11-18",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_460"
}

Velkovski, S. D., Mazić, S., Nešić, D. M., Igrački, I., Milošević, V.,& Starčević, V. P.. (2002). Povećana agregabilnost trombocita posle aterogene dijete u kunića. in Acta veterinaria, 52(1), 11-18.
https://hdl.handle.net/21.15107/rcub_ibiss_460

Velkovski SD, Mazić S, Nešić DM, Igrački I, Milošević V, Starčević VP. Povećana agregabilnost trombocita posle aterogene dijete u kunića. in Acta veterinaria. 2002;52(1):11-18.
https://hdl.handle.net/21.15107/rcub_ibiss_460 .

Velkovski, Saško D., Mazić, Sanja, Nešić, Dejan M., Igrački, Iva, Milošević, Verica, Starčević, Vesna P., "Povećana agregabilnost trombocita posle aterogene dijete u kunića" in Acta veterinaria, 52, no. 1 (2002):11-18,
https://hdl.handle.net/21.15107/rcub_ibiss_460 .