TY  - JOUR
AU  - Drakulić, Danijela D
AU  - Jovicić, Dubravka
AU  - Milacić, Snezana B
AU  - Vukov, Tanja
AU  - Rakić, Boban M
AU  - Stevanović, Milena J
AU  - Rakić, Rada S
AU  - Bukvić, Nenad M
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1381
AB  - We have analyzed the frequency of premature centromeric division (PCD) in medical personnel professionally exposed to low doses of radiation. They had chromosome aberrations (CAs) involving dicentric chromosomes, ring chromosomes, acentric fragments, chromosome breaks, and chromatid breaks. The study included 30 exposed subjects and 23 controls who were each analyzed by a conventional cytogenetics procedure and subsequently by fluorescent in situ hybridization ( FISH). The latter was applied particularly in order to verify PCD in a specific chromosome ( chromosome 18) in both metaphases and interphase nuclei. The results revealed a significant difference (p < 0.001) in frequencies between the two groups ( exposed and controls) for all the observed variables ( CAs), metaphases with PCD (MPCD), total number of chromosomes with PCD (TPCD), number of PCD metaphases in acrocentric chromosomes (MAPCD), and the total number of acrocentric chromosomes with PCD (TAPCD). The doses of ionizing radiation absorbed by the subjects' bodies were measured with thermoluminescent dosimeters once a month during the duration of occupational exposure. They were expressed in mSv, as mean annual effective doses for the period of exposure. The Spearman rank test showed a high positive correlation between total life effective dose and frequency of CAs and PCD. Based on the results obtained in this study, we suggest that PCD, as a phenomenon manifesting chromosomal instability (CIN), should be considered as a suitable cytogenetic biomarker for individuals occupationally exposed to ionizing radiation. Health Phys. 98(5):717-726; 2010
T2  - Health Physics
T1  - Detection of Premature Segregation of Centromeres in Persons Exposed To Ionizing Radiation
IS  - 5
VL  - 98
EP  - 726
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1381
ER  - 

@article{
author = "Drakulić, Danijela D and Jovicić, Dubravka and Milacić, Snezana B and Vukov, Tanja and Rakić, Boban M and Stevanović, Milena J and Rakić, Rada S and Bukvić, Nenad M",
year = "2010",
abstract = "We have analyzed the frequency of premature centromeric division (PCD) in medical personnel professionally exposed to low doses of radiation. They had chromosome aberrations (CAs) involving dicentric chromosomes, ring chromosomes, acentric fragments, chromosome breaks, and chromatid breaks. The study included 30 exposed subjects and 23 controls who were each analyzed by a conventional cytogenetics procedure and subsequently by fluorescent in situ hybridization ( FISH). The latter was applied particularly in order to verify PCD in a specific chromosome ( chromosome 18) in both metaphases and interphase nuclei. The results revealed a significant difference (p < 0.001) in frequencies between the two groups ( exposed and controls) for all the observed variables ( CAs), metaphases with PCD (MPCD), total number of chromosomes with PCD (TPCD), number of PCD metaphases in acrocentric chromosomes (MAPCD), and the total number of acrocentric chromosomes with PCD (TAPCD). The doses of ionizing radiation absorbed by the subjects' bodies were measured with thermoluminescent dosimeters once a month during the duration of occupational exposure. They were expressed in mSv, as mean annual effective doses for the period of exposure. The Spearman rank test showed a high positive correlation between total life effective dose and frequency of CAs and PCD. Based on the results obtained in this study, we suggest that PCD, as a phenomenon manifesting chromosomal instability (CIN), should be considered as a suitable cytogenetic biomarker for individuals occupationally exposed to ionizing radiation. Health Phys. 98(5):717-726; 2010",
journal = "Health Physics",
title = "Detection of Premature Segregation of Centromeres in Persons Exposed To Ionizing Radiation",
number = "5",
volume = "98",
pages = "726",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1381"
}

Drakulić, D. D., Jovicić, D., Milacić, S. B., Vukov, T., Rakić, B. M., Stevanović, M. J., Rakić, R. S.,& Bukvić, N. M.. (2010). Detection of Premature Segregation of Centromeres in Persons Exposed To Ionizing Radiation. in Health Physics, 98(5).
https://hdl.handle.net/21.15107/rcub_ibiss_1381

Drakulić DD, Jovicić D, Milacić SB, Vukov T, Rakić BM, Stevanović MJ, Rakić RS, Bukvić NM. Detection of Premature Segregation of Centromeres in Persons Exposed To Ionizing Radiation. in Health Physics. 2010;98(5):null-726.
https://hdl.handle.net/21.15107/rcub_ibiss_1381 .

Drakulić, Danijela D, Jovicić, Dubravka, Milacić, Snezana B, Vukov, Tanja, Rakić, Boban M, Stevanović, Milena J, Rakić, Rada S, Bukvić, Nenad M, "Detection of Premature Segregation of Centromeres in Persons Exposed To Ionizing Radiation" in Health Physics, 98, no. 5 (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1381 .

TY  - JOUR
AU  - Jovicić, Dubravka
AU  - Milacić, Snezana B
AU  - Milić, Natasa M
AU  - Bukvić, Nenad M
AU  - Vukov, Tanja
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1481
AB  - Occupational exposure to low doses of ionizing radiation is a particularly delicate subject for investigation, due to the cumulative effects of chronic exposure. It is extremely important to consider and to measure the biological response to given conditions of exposure. The aim of this study was to establish possible recovery from DNA damage in subjects professionally exposed to radiation in their working area by examinations for chromosomal aberrations (CA) at two different times. The first group (I) was composed of 30 professionally exposed subjects in whom unstable CA (dicentrics, ring, acentric fragments, chromatid, chromosomal breaks, and chromatid interchanges) were identified at time zero. After removal from the radiation area, they were re-examined 9 months later. The second group (H) contained 64 healthy individuals, not professionally exposed to ionizing radiation or other known mutagenic agents. In the group of exposed individuals, five (16.67%)) subjects exhibited permanent unstable CAs, even after 9 months absence from the radiation. When the nonexposed and exposed groups were compared, an increase of unstable aberrations (p < 0.05) was observed in the exposed group. Nevertheless, a statistically significant decrease of dicentrics, acentric fragments, and ring frequencies was observed in exposed individuals after 9 months away from the radiation area. However, chromatid and isochromatid break frequencies increased slightly but not significantly after 9 months. The detected CAs corresponded to the total effective doses of radiation measured in our subjects. The existence of CAs in some individuals even after absence from the radiation area suggests that the time necessary for the damaged DNA to recover is extremely variable and indicates interindividual differences in radiosensitivity as well as differences in the cellular-reparation response.
T2  - Journal of Environmental Pathology Toxicology and Oncology
T1  - Chromosomal Aberrations in Subjects Exposed to Ionizing Radiation
IS  - 1
VL  - 28
EP  - 82
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1481
ER  - 

@article{
author = "Jovicić, Dubravka and Milacić, Snezana B and Milić, Natasa M and Bukvić, Nenad M and Vukov, Tanja",
year = "2009",
abstract = "Occupational exposure to low doses of ionizing radiation is a particularly delicate subject for investigation, due to the cumulative effects of chronic exposure. It is extremely important to consider and to measure the biological response to given conditions of exposure. The aim of this study was to establish possible recovery from DNA damage in subjects professionally exposed to radiation in their working area by examinations for chromosomal aberrations (CA) at two different times. The first group (I) was composed of 30 professionally exposed subjects in whom unstable CA (dicentrics, ring, acentric fragments, chromatid, chromosomal breaks, and chromatid interchanges) were identified at time zero. After removal from the radiation area, they were re-examined 9 months later. The second group (H) contained 64 healthy individuals, not professionally exposed to ionizing radiation or other known mutagenic agents. In the group of exposed individuals, five (16.67%)) subjects exhibited permanent unstable CAs, even after 9 months absence from the radiation. When the nonexposed and exposed groups were compared, an increase of unstable aberrations (p < 0.05) was observed in the exposed group. Nevertheless, a statistically significant decrease of dicentrics, acentric fragments, and ring frequencies was observed in exposed individuals after 9 months away from the radiation area. However, chromatid and isochromatid break frequencies increased slightly but not significantly after 9 months. The detected CAs corresponded to the total effective doses of radiation measured in our subjects. The existence of CAs in some individuals even after absence from the radiation area suggests that the time necessary for the damaged DNA to recover is extremely variable and indicates interindividual differences in radiosensitivity as well as differences in the cellular-reparation response.",
journal = "Journal of Environmental Pathology Toxicology and Oncology",
title = "Chromosomal Aberrations in Subjects Exposed to Ionizing Radiation",
number = "1",
volume = "28",
pages = "82",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1481"
}

Jovicić, D., Milacić, S. B., Milić, N. M., Bukvić, N. M.,& Vukov, T.. (2009). Chromosomal Aberrations in Subjects Exposed to Ionizing Radiation. in Journal of Environmental Pathology Toxicology and Oncology, 28(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1481

Jovicić D, Milacić SB, Milić NM, Bukvić NM, Vukov T. Chromosomal Aberrations in Subjects Exposed to Ionizing Radiation. in Journal of Environmental Pathology Toxicology and Oncology. 2009;28(1):null-82.
https://hdl.handle.net/21.15107/rcub_ibiss_1481 .

Jovicić, Dubravka, Milacić, Snezana B, Milić, Natasa M, Bukvić, Nenad M, Vukov, Tanja, "Chromosomal Aberrations in Subjects Exposed to Ionizing Radiation" in Journal of Environmental Pathology Toxicology and Oncology, 28, no. 1 (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1481 .