TY  - JOUR
AU  - Tanić, Nikola T
AU  - Stanojević, Boban R
AU  - Tanić, Nasta
AU  - Schaefer, Stephan
AU  - Niesters, Hubert GM
AU  - Bozić, Milena
AU  - Dimitrijević, Bogomir B.
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1424
AB  - Hepatitis B virus (HBV) infection is a global health problem associated with severe liver disorders. Viral load and HBV genotype affect the clinical outcome, guide antiviral therapy and provide long term prognosis for HBV infected patients. Various types of detection and quantitation assays are currently in use with a different effectiveness. The aim of this study was to develop a method that would provide simultaneous identification and quantitation of genotypes A and D in a single-tube reaction. Sera from infected patients were analyzed by a TaqMan based real time PCR. Optimized reagents were used for HBV DNA quantitation while the genotypes A and D were quantified separately by our design of the assay. Multiplex real time PCR was achieved and was specific for HBV genotypes A and D within a single-tube reaction. Simulation of mixed virus populations was identified reproducibly in vitro. Quantitation of these individual genotypes was exceptionally reliable, so much so that the sum of individual genotypes was equal to the total viral load determined in a separate reaction. Therefore, a straightforward, conceptually simple and reliable approach to issues involving HBV genotypes A and D is submitted. Identity and exact titer of these genotypes in the Caucasian population can now be determined easily. (C) 2009 Elsevier B.V. All rights reserved.
T2  - Journal of Virological Methods
T1  - Concurrent quantitation of the A and D genotypes of hepatitis B virus
IS  - 2
VL  - 161
EP  - 270
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1424
ER  - 

@article{
author = "Tanić, Nikola T and Stanojević, Boban R and Tanić, Nasta and Schaefer, Stephan and Niesters, Hubert GM and Bozić, Milena and Dimitrijević, Bogomir B.",
year = "2009",
abstract = "Hepatitis B virus (HBV) infection is a global health problem associated with severe liver disorders. Viral load and HBV genotype affect the clinical outcome, guide antiviral therapy and provide long term prognosis for HBV infected patients. Various types of detection and quantitation assays are currently in use with a different effectiveness. The aim of this study was to develop a method that would provide simultaneous identification and quantitation of genotypes A and D in a single-tube reaction. Sera from infected patients were analyzed by a TaqMan based real time PCR. Optimized reagents were used for HBV DNA quantitation while the genotypes A and D were quantified separately by our design of the assay. Multiplex real time PCR was achieved and was specific for HBV genotypes A and D within a single-tube reaction. Simulation of mixed virus populations was identified reproducibly in vitro. Quantitation of these individual genotypes was exceptionally reliable, so much so that the sum of individual genotypes was equal to the total viral load determined in a separate reaction. Therefore, a straightforward, conceptually simple and reliable approach to issues involving HBV genotypes A and D is submitted. Identity and exact titer of these genotypes in the Caucasian population can now be determined easily. (C) 2009 Elsevier B.V. All rights reserved.",
journal = "Journal of Virological Methods",
title = "Concurrent quantitation of the A and D genotypes of hepatitis B virus",
number = "2",
volume = "161",
pages = "270",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1424"
}

Tanić, N. T., Stanojević, B. R., Tanić, N., Schaefer, S., Niesters, H. G., Bozić, M.,& Dimitrijević, B. B.. (2009). Concurrent quantitation of the A and D genotypes of hepatitis B virus. in Journal of Virological Methods, 161(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1424

Tanić NT, Stanojević BR, Tanić N, Schaefer S, Niesters HG, Bozić M, Dimitrijević BB. Concurrent quantitation of the A and D genotypes of hepatitis B virus. in Journal of Virological Methods. 2009;161(2):null-270.
https://hdl.handle.net/21.15107/rcub_ibiss_1424 .

Tanić, Nikola T, Stanojević, Boban R, Tanić, Nasta, Schaefer, Stephan, Niesters, Hubert GM, Bozić, Milena, Dimitrijević, Bogomir B., "Concurrent quantitation of the A and D genotypes of hepatitis B virus" in Journal of Virological Methods, 161, no. 2 (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1424 .