TY  - JOUR
AU  - Grubor-Lajšić, Gordana
AU  - Petri, Edward
AU  - Kojić, Danijela
AU  - Purać, Jelena
AU  - Popović, Željko
AU  - Worland, Michael Roger
AU  - Clark, Melody S.
AU  - Mojović, Miloš
AU  - Blagojević, Duško
PY  - 2013
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6338
AB  - The Arctic springtail, Megaphorura arctica, survives sub-zero temperatures in a dehydrated state via trehalose-dependent cryoprotective dehydration. Regulation of trehalose biosynthesis is complex; based in part on studies in yeast and fungi, its connection with oxidative stress caused by exposure of cells to oxidants, such as hydrogen peroxide (H2O2), or dehydration, is well documented. In this respect, we measured the amount of H2O2 and antioxidant enzyme activities (superoxide dismutases: copper, zinc—CuZnSOD and manganese containing–MnSOD, and catalase—CAT), as the regulatory components determining H2O2 concentrations, in Arctic springtails incubated at 5 ◦C (control) versus −2 ◦C (threshold temperature for trehalose biosynthesis). Because ecdysone also stimulates trehalose production in insects and regulates the expression of genes involved in redox homeostasis and antioxidant protection in
Drosophila, we measured the levels of the active physiological form of ecdysone—20-hydroxyecdysone (20-HE). Significantly elevated H2O2 and 20-HE levels were observed in M. arctica incubated at −2 ◦C, supporting a link between ecdysone, H2O2, and trehalose levels during cryoprotective dehydration. CAT activity was found to be significantly lower in M. arctica incubated at −2 ◦C versus 5 ◦C, suggesting reduced H2O2 breakdown. Furthermore, measurement of the free radical composition in Arctic springtails incubated at 5 ◦C (controls) versus −2 ◦C by Electron Paramagnetic Resonance spectroscopy revealed melanin-derived free radicals at −2 ◦C, perhaps an additional source of H2O2. Our results suggest that H2O2 and ecdysone play important roles in the cryoprotective dehydration process in M. arctica, linked with the regulation of trehalose biosynthesis.
PB  - Hoboken: John Wiley and Sons
T2  - Archives of Insect Biochemistry and Physiology
T1  - Hydrogen peroxide and ecdysone in the cryoprotective dehydration strategy of Megaphorura Arctica (Onychiuridae: Collembola)
IS  - 2
VL  - 82
DO  - 10.1002/arch.21073
SP  - 59
EP  - 70
ER  - 

@article{
author = "Grubor-Lajšić, Gordana and Petri, Edward and Kojić, Danijela and Purać, Jelena and Popović, Željko and Worland, Michael Roger and Clark, Melody S. and Mojović, Miloš and Blagojević, Duško",
year = "2013",
abstract = "The Arctic springtail, Megaphorura arctica, survives sub-zero temperatures in a dehydrated state via trehalose-dependent cryoprotective dehydration. Regulation of trehalose biosynthesis is complex; based in part on studies in yeast and fungi, its connection with oxidative stress caused by exposure of cells to oxidants, such as hydrogen peroxide (H2O2), or dehydration, is well documented. In this respect, we measured the amount of H2O2 and antioxidant enzyme activities (superoxide dismutases: copper, zinc—CuZnSOD and manganese containing–MnSOD, and catalase—CAT), as the regulatory components determining H2O2 concentrations, in Arctic springtails incubated at 5 ◦C (control) versus −2 ◦C (threshold temperature for trehalose biosynthesis). Because ecdysone also stimulates trehalose production in insects and regulates the expression of genes involved in redox homeostasis and antioxidant protection in
Drosophila, we measured the levels of the active physiological form of ecdysone—20-hydroxyecdysone (20-HE). Significantly elevated H2O2 and 20-HE levels were observed in M. arctica incubated at −2 ◦C, supporting a link between ecdysone, H2O2, and trehalose levels during cryoprotective dehydration. CAT activity was found to be significantly lower in M. arctica incubated at −2 ◦C versus 5 ◦C, suggesting reduced H2O2 breakdown. Furthermore, measurement of the free radical composition in Arctic springtails incubated at 5 ◦C (controls) versus −2 ◦C by Electron Paramagnetic Resonance spectroscopy revealed melanin-derived free radicals at −2 ◦C, perhaps an additional source of H2O2. Our results suggest that H2O2 and ecdysone play important roles in the cryoprotective dehydration process in M. arctica, linked with the regulation of trehalose biosynthesis.",
publisher = "Hoboken: John Wiley and Sons",
journal = "Archives of Insect Biochemistry and Physiology",
title = "Hydrogen peroxide and ecdysone in the cryoprotective dehydration strategy of Megaphorura Arctica (Onychiuridae: Collembola)",
number = "2",
volume = "82",
doi = "10.1002/arch.21073",
pages = "59-70"
}

Grubor-Lajšić, G., Petri, E., Kojić, D., Purać, J., Popović, Ž., Worland, M. R., Clark, M. S., Mojović, M.,& Blagojević, D.. (2013). Hydrogen peroxide and ecdysone in the cryoprotective dehydration strategy of Megaphorura Arctica (Onychiuridae: Collembola). in Archives of Insect Biochemistry and Physiology
Hoboken: John Wiley and Sons., 82(2), 59-70.
https://doi.org/10.1002/arch.21073

Grubor-Lajšić G, Petri E, Kojić D, Purać J, Popović Ž, Worland MR, Clark MS, Mojović M, Blagojević D. Hydrogen peroxide and ecdysone in the cryoprotective dehydration strategy of Megaphorura Arctica (Onychiuridae: Collembola). in Archives of Insect Biochemistry and Physiology. 2013;82(2):59-70.
doi:10.1002/arch.21073 .

Grubor-Lajšić, Gordana, Petri, Edward, Kojić, Danijela, Purać, Jelena, Popović, Željko, Worland, Michael Roger, Clark, Melody S., Mojović, Miloš, Blagojević, Duško, "Hydrogen peroxide and ecdysone in the cryoprotective dehydration strategy of Megaphorura Arctica (Onychiuridae: Collembola)" in Archives of Insect Biochemistry and Physiology, 82, no. 2 (2013):59-70,
https://doi.org/10.1002/arch.21073 . .

TY  - JOUR
AU  - Vukašinović, Elvira
AU  - Pond, David
AU  - Worland, Michael Roger
AU  - Kojić, Danijela
AU  - Purać, Jelena
AU  - Blagojević, Duško
AU  - Grubor-Lajšić, Gordana
PY  - 2013
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6337
AB  - This study compares the composition and biophysical properties of lipids in non-diapausing and diapausing
fifth instar larvae of Ostrinia nubilalis Hubn. (Lepidoptera: Crambidae). The majority of fat body lipids in both of these physiological states were comprised of ~90% triacylglycerols (TAGs), whereas the haemolymph contained a more even distribution of all lipid classes. The fatty acid composition and biophysical properties of the fat body lipids differed markedly between non-diapausing and diapausing larvae. Diapause was associated with a dramatic increase in the proportions of palmitoleic acid (16:1n-7) and oleic acid (18:1n-9), with concurrent reductions in palmitic acid (16:0) and linoleic acid (18:2n-6). The increase in the level of unsaturation of the fat body lipids, which caused a marked shift in their phase transitions to lower temperatures, was triggered by diapause rather than low temperatures. Adjustments of fatty acid compositions are likely to be an important component of winter diapause mechanisms, possibly maintaining the fluidity of cell membranes and the functionality of the organism during lower winter temperatures.
PB  - Elsevier Inc.
T2  - Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology
T1  - Diapause induces changes in the composition and biophysical properties of lipids in larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae)
IS  - 4
VL  - 165
DO  - 10.1016/j.cbpb.2013.05.001
SP  - 219
EP  - 225
ER  - 

@article{
author = "Vukašinović, Elvira and Pond, David and Worland, Michael Roger and Kojić, Danijela and Purać, Jelena and Blagojević, Duško and Grubor-Lajšić, Gordana",
year = "2013",
abstract = "This study compares the composition and biophysical properties of lipids in non-diapausing and diapausing
fifth instar larvae of Ostrinia nubilalis Hubn. (Lepidoptera: Crambidae). The majority of fat body lipids in both of these physiological states were comprised of ~90% triacylglycerols (TAGs), whereas the haemolymph contained a more even distribution of all lipid classes. The fatty acid composition and biophysical properties of the fat body lipids differed markedly between non-diapausing and diapausing larvae. Diapause was associated with a dramatic increase in the proportions of palmitoleic acid (16:1n-7) and oleic acid (18:1n-9), with concurrent reductions in palmitic acid (16:0) and linoleic acid (18:2n-6). The increase in the level of unsaturation of the fat body lipids, which caused a marked shift in their phase transitions to lower temperatures, was triggered by diapause rather than low temperatures. Adjustments of fatty acid compositions are likely to be an important component of winter diapause mechanisms, possibly maintaining the fluidity of cell membranes and the functionality of the organism during lower winter temperatures.",
publisher = "Elsevier Inc.",
journal = "Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology",
title = "Diapause induces changes in the composition and biophysical properties of lipids in larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae)",
number = "4",
volume = "165",
doi = "10.1016/j.cbpb.2013.05.001",
pages = "219-225"
}

Vukašinović, E., Pond, D., Worland, M. R., Kojić, D., Purać, J., Blagojević, D.,& Grubor-Lajšić, G.. (2013). Diapause induces changes in the composition and biophysical properties of lipids in larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae). in Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology
Elsevier Inc.., 165(4), 219-225.
https://doi.org/10.1016/j.cbpb.2013.05.001

Vukašinović E, Pond D, Worland MR, Kojić D, Purać J, Blagojević D, Grubor-Lajšić G. Diapause induces changes in the composition and biophysical properties of lipids in larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae). in Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 2013;165(4):219-225.
doi:10.1016/j.cbpb.2013.05.001 .

Vukašinović, Elvira, Pond, David, Worland, Michael Roger, Kojić, Danijela, Purać, Jelena, Blagojević, Duško, Grubor-Lajšić, Gordana, "Diapause induces changes in the composition and biophysical properties of lipids in larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae)" in Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 165, no. 4 (2013):219-225,
https://doi.org/10.1016/j.cbpb.2013.05.001 . .