Selaković, Nenad

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  • Selaković, Nenad (2)
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Diagnostics of atmospheric pressure plasmas and their application in agriculture

Puač, Nevena; Škoro, Nikola; Maletić, Dejan; Živković, Suzana; Selaković, Nenad; Malović, Gordana; Petrović, Zoran Lj

(Bratislava: Department of Experimental Physics, Faculty of Mathematics, Physics and Informatics, Comenius University in Bratislava, 2019)

TY  - CONF
AU  - Puač, Nevena
AU  - Škoro, Nikola
AU  - Maletić, Dejan
AU  - Živković, Suzana
AU  - Selaković, Nenad
AU  - Malović, Gordana
AU  - Petrović, Zoran Lj
PY  - 2019
UR  - http://neon.dpp.fmph.uniba.sk/sappxxii/download/SAPP_XXII_JSPP_XI_Book_of_Contributed_Papers.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6199
AB  - Atmospheric pressure plasmas (APPs) have been intensively studied in the last decade due to their high
potential in the applications in medicine, biology and, lately, in agriculture. They are generated in plasma sources of various geometries and configurations and with different electrode material in order to make them efficient for large variety of applications. The types of sources used are mainly atmospheric pressure plasma jets (APPJs), but since some applications require large areas to be treated plan parallel dielectric barrier discharges [3-6] are also utilized. Typical configurations of APPJs consist of a tube for conducting the flow of the buffer gas and set of electrodes where powered one can be in the contact with the plasma or covered by dielectric. The power supplies that are most commonly used can be divided by the type of signal as continuous or pulsed and they can operate in the large range of frequencies (from several kHz to GHz). The choice of the geometry as well as the type of the power supply used is governed by the application of the APP. Regardless of the system choice and configuration, one needs to perform detailed diagnostics of the plasma system to obtain the data that could be linked to the processes significant for the particular treatment. Then, the efficiency of desired application can be assessed and, which is also important in many cases, there is an opportunity up to some extent to compare the achieved effects with the other systems used for the same application. At the same time, the detailed diagnostics allows the studies of fundamental questions of APP behaviour. For instance, in the case of APPJs an interesting feature of formation of pulsed atmospheric-pressure streamers (PAPS) was observed and investigated. The fast ICCD imaging of this phenomena revealed that PAPS have a speed of several kilometres per second and their formation and propagation still needs to be explained in more detail. However, from the point of applications the most important feature of the APP is that they create chemically highly active media (both in gas and liquid) with the properties that can be generally tuned according to the application’s demands. The active chemistry of the plasma’s gas phase can directly modify the treated surface or activate specific mechanisms inside the treated target, e.g. plasma
treatment of cells leading to the improved differentiation or cancer cell death. Also, since APP can operate in direct contact with a liquid, the chemically active environment produced in the gas phase above the liquid can modify the physical and chemical properties of the treated liquid. Extensive research in this type of experimental configurations has led to the development of the applications of
APP in the field of agriculture. Two examples of such applications are direct treatment of seeds and
treatment of water in order to create plasma activated water. It was shown that in both cases plasma
treatment induces higher germination percentage, less contamination of the seedlings, higher water
uptake and faster plant development. These and other observed effects are the result of the interaction
of plasma formed reactive oxygen and nitrogen species (RONS) with the seed and plant cells. RONS
can trigger various biochemical mechanisms that can be observed also at molecular level through the
activity of enzymes or hormones in the seeds and plants. Here we will try to give the overview of the detailed characterization of the APP systems that were used for both medical applications and applications in agriculture. Results of time-resolved plasma imaging using fast ICCD camera will show the development of plasma structure within one period of the power signal and provide an insight in kinetic effects such as PAPS. Moreover, by using optical emission spectroscopy spectra of excited species in the gas phase will be obtained allowing the qualitative assessment of excited species above the treated liquid. The results of electrical measurements of APP sources, as another important diagnostics tool, will be presented. Comparison of the two different APP (in electrode geometry and applied voltage frequency) will be done by comparing both the results of plasma diagnostics and the response of the biological system treated by these plasma sources. Also, investigations of the applications of APP in the agriculture will be shown featuring the idea of plasma decontamination of water polluted by pesticides and its influence on germination of commercial plants.
PB  - Bratislava: Department of Experimental Physics, Faculty of Mathematics, Physics and Informatics, Comenius University in Bratislava
C3  - Book of Contributed Papers: 22nd Symposium on Application of Plasma Processes and 11th EU-Japan Joint Symposium on Plasma Processing; 2019 Jan 18-24; Štrbské Pleso, Slovakia
T1  - Diagnostics of atmospheric pressure plasmas and their application in agriculture
SP  - 68
EP  - 69
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6199
ER  - 
@conference{
author = "Puač, Nevena and Škoro, Nikola and Maletić, Dejan and Živković, Suzana and Selaković, Nenad and Malović, Gordana and Petrović, Zoran Lj",
year = "2019",
abstract = "Atmospheric pressure plasmas (APPs) have been intensively studied in the last decade due to their high
potential in the applications in medicine, biology and, lately, in agriculture. They are generated in plasma sources of various geometries and configurations and with different electrode material in order to make them efficient for large variety of applications. The types of sources used are mainly atmospheric pressure plasma jets (APPJs), but since some applications require large areas to be treated plan parallel dielectric barrier discharges [3-6] are also utilized. Typical configurations of APPJs consist of a tube for conducting the flow of the buffer gas and set of electrodes where powered one can be in the contact with the plasma or covered by dielectric. The power supplies that are most commonly used can be divided by the type of signal as continuous or pulsed and they can operate in the large range of frequencies (from several kHz to GHz). The choice of the geometry as well as the type of the power supply used is governed by the application of the APP. Regardless of the system choice and configuration, one needs to perform detailed diagnostics of the plasma system to obtain the data that could be linked to the processes significant for the particular treatment. Then, the efficiency of desired application can be assessed and, which is also important in many cases, there is an opportunity up to some extent to compare the achieved effects with the other systems used for the same application. At the same time, the detailed diagnostics allows the studies of fundamental questions of APP behaviour. For instance, in the case of APPJs an interesting feature of formation of pulsed atmospheric-pressure streamers (PAPS) was observed and investigated. The fast ICCD imaging of this phenomena revealed that PAPS have a speed of several kilometres per second and their formation and propagation still needs to be explained in more detail. However, from the point of applications the most important feature of the APP is that they create chemically highly active media (both in gas and liquid) with the properties that can be generally tuned according to the application’s demands. The active chemistry of the plasma’s gas phase can directly modify the treated surface or activate specific mechanisms inside the treated target, e.g. plasma
treatment of cells leading to the improved differentiation or cancer cell death. Also, since APP can operate in direct contact with a liquid, the chemically active environment produced in the gas phase above the liquid can modify the physical and chemical properties of the treated liquid. Extensive research in this type of experimental configurations has led to the development of the applications of
APP in the field of agriculture. Two examples of such applications are direct treatment of seeds and
treatment of water in order to create plasma activated water. It was shown that in both cases plasma
treatment induces higher germination percentage, less contamination of the seedlings, higher water
uptake and faster plant development. These and other observed effects are the result of the interaction
of plasma formed reactive oxygen and nitrogen species (RONS) with the seed and plant cells. RONS
can trigger various biochemical mechanisms that can be observed also at molecular level through the
activity of enzymes or hormones in the seeds and plants. Here we will try to give the overview of the detailed characterization of the APP systems that were used for both medical applications and applications in agriculture. Results of time-resolved plasma imaging using fast ICCD camera will show the development of plasma structure within one period of the power signal and provide an insight in kinetic effects such as PAPS. Moreover, by using optical emission spectroscopy spectra of excited species in the gas phase will be obtained allowing the qualitative assessment of excited species above the treated liquid. The results of electrical measurements of APP sources, as another important diagnostics tool, will be presented. Comparison of the two different APP (in electrode geometry and applied voltage frequency) will be done by comparing both the results of plasma diagnostics and the response of the biological system treated by these plasma sources. Also, investigations of the applications of APP in the agriculture will be shown featuring the idea of plasma decontamination of water polluted by pesticides and its influence on germination of commercial plants.",
publisher = "Bratislava: Department of Experimental Physics, Faculty of Mathematics, Physics and Informatics, Comenius University in Bratislava",
journal = "Book of Contributed Papers: 22nd Symposium on Application of Plasma Processes and 11th EU-Japan Joint Symposium on Plasma Processing; 2019 Jan 18-24; Štrbské Pleso, Slovakia",
title = "Diagnostics of atmospheric pressure plasmas and their application in agriculture",
pages = "68-69",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6199"
}
Puač, N., Škoro, N., Maletić, D., Živković, S., Selaković, N., Malović, G.,& Petrović, Z. L.. (2019). Diagnostics of atmospheric pressure plasmas and their application in agriculture. in Book of Contributed Papers: 22nd Symposium on Application of Plasma Processes and 11th EU-Japan Joint Symposium on Plasma Processing; 2019 Jan 18-24; Štrbské Pleso, Slovakia
Bratislava: Department of Experimental Physics, Faculty of Mathematics, Physics and Informatics, Comenius University in Bratislava., 68-69.
https://hdl.handle.net/21.15107/rcub_ibiss_6199
Puač N, Škoro N, Maletić D, Živković S, Selaković N, Malović G, Petrović ZL. Diagnostics of atmospheric pressure plasmas and their application in agriculture. in Book of Contributed Papers: 22nd Symposium on Application of Plasma Processes and 11th EU-Japan Joint Symposium on Plasma Processing; 2019 Jan 18-24; Štrbské Pleso, Slovakia. 2019;:68-69.
https://hdl.handle.net/21.15107/rcub_ibiss_6199 .
Puač, Nevena, Škoro, Nikola, Maletić, Dejan, Živković, Suzana, Selaković, Nenad, Malović, Gordana, Petrović, Zoran Lj, "Diagnostics of atmospheric pressure plasmas and their application in agriculture" in Book of Contributed Papers: 22nd Symposium on Application of Plasma Processes and 11th EU-Japan Joint Symposium on Plasma Processing; 2019 Jan 18-24; Štrbské Pleso, Slovakia (2019):68-69,
https://hdl.handle.net/21.15107/rcub_ibiss_6199 .

The effects of atmospheric pressure plasma on somatic embryogenesis of carrot (Daucus carrota)

Selaković, Nenad; Jevremović, Slađana; Živković, Suzana; Maletić, Dejan; Puač, Nevena; Malović, Gordana; Petrović, Zoran Lj

(Belgrade: Serbian Plant Physiology Society, 2013)

TY  - CONF
AU  - Selaković, Nenad
AU  - Jevremović, Slađana
AU  - Živković, Suzana
AU  - Maletić, Dejan
AU  - Puač, Nevena
AU  - Malović, Gordana
AU  - Petrović, Zoran Lj
PY  - 2013
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6396
AB  - The effects of atmospheric pressure plasmas on living cells and tissues have been studied on numerous occasions in recent literature. lt appears that plasma treatment may find many biomedical applications. There are many types of plasmas that can be generated under ambient pressure and temperature conditions. Plas­ma needle is one of the atmospheric pressure devices that meet the requirements of precise and localized treatment necessary for treatment of plant cells. We have investigated the effect of atmospheric pressure plasma generated by plasma needle device, under ambient pressure and temperature conditions, on carrot somatic embryogenesis. The embryogenic calli cul­tures of carrot (Daucus carrota) were established from storage root explants and maintained on Murashige and Skoog medium (MS) supplemented with 2,4-dichlorphenoxyacetic acid (2,4-D, 1.0 mg L·1) and cultured in dark. Calli samples (10-30 mg) were placed in 96 wells micro-titer plate before the tip of the plasma needle was placed at the edge of the well when plasma was covering whole surface of the samples. The treatment times were 1 O, 30, 60 and 120 s at two powers, namely 0.4 W and 1.4 W. After plasma treatment, calli samples were cultured on basal MS medium without plant growth regulators or on MS medium supplemented with 2,4-D or 2,4-D and kinetin (1.0 mg L-1, each) and cultured in dark for six weeks. Calli number, fresh weight in­crease and developmental stage of formed somatic embryos were estimated. lt was shown that plasma treat­ment notably stimulated growth and somatic embryo formation of calli cultured on basal MS medium. The highest fresh weight increase (-40 fold) was observed after 30 s of plasma needle treatment at 0.4 W. The number and developmental stage of formed somatic embryos depended on duration and power of plasma treatment, as well as the type of culture media. The highest number of somatic embryos was observed when calli samples were grown on basal MS medium after 60 s of plasma treatment at 1.4 W. Furthermore, plasma treatment significantly increased the number of formed somatic embryos on MS media supplemented with plant growth regulators. Our results show that plasma strongly affects growth and somatic embryo forma­tion and development of carrot calli.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia
T1  - The effects of atmospheric pressure plasma on somatic embryogenesis  of carrot (Daucus carrota)
SP  - 36
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6396
ER  - 
@conference{
author = "Selaković, Nenad and Jevremović, Slađana and Živković, Suzana and Maletić, Dejan and Puač, Nevena and Malović, Gordana and Petrović, Zoran Lj",
year = "2013",
abstract = "The effects of atmospheric pressure plasmas on living cells and tissues have been studied on numerous occasions in recent literature. lt appears that plasma treatment may find many biomedical applications. There are many types of plasmas that can be generated under ambient pressure and temperature conditions. Plas­ma needle is one of the atmospheric pressure devices that meet the requirements of precise and localized treatment necessary for treatment of plant cells. We have investigated the effect of atmospheric pressure plasma generated by plasma needle device, under ambient pressure and temperature conditions, on carrot somatic embryogenesis. The embryogenic calli cul­tures of carrot (Daucus carrota) were established from storage root explants and maintained on Murashige and Skoog medium (MS) supplemented with 2,4-dichlorphenoxyacetic acid (2,4-D, 1.0 mg L·1) and cultured in dark. Calli samples (10-30 mg) were placed in 96 wells micro-titer plate before the tip of the plasma needle was placed at the edge of the well when plasma was covering whole surface of the samples. The treatment times were 1 O, 30, 60 and 120 s at two powers, namely 0.4 W and 1.4 W. After plasma treatment, calli samples were cultured on basal MS medium without plant growth regulators or on MS medium supplemented with 2,4-D or 2,4-D and kinetin (1.0 mg L-1, each) and cultured in dark for six weeks. Calli number, fresh weight in­crease and developmental stage of formed somatic embryos were estimated. lt was shown that plasma treat­ment notably stimulated growth and somatic embryo formation of calli cultured on basal MS medium. The highest fresh weight increase (-40 fold) was observed after 30 s of plasma needle treatment at 0.4 W. The number and developmental stage of formed somatic embryos depended on duration and power of plasma treatment, as well as the type of culture media. The highest number of somatic embryos was observed when calli samples were grown on basal MS medium after 60 s of plasma treatment at 1.4 W. Furthermore, plasma treatment significantly increased the number of formed somatic embryos on MS media supplemented with plant growth regulators. Our results show that plasma strongly affects growth and somatic embryo forma­tion and development of carrot calli.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia",
title = "The effects of atmospheric pressure plasma on somatic embryogenesis  of carrot (Daucus carrota)",
pages = "36",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6396"
}
Selaković, N., Jevremović, S., Živković, S., Maletić, D., Puač, N., Malović, G.,& Petrović, Z. L.. (2013). The effects of atmospheric pressure plasma on somatic embryogenesis  of carrot (Daucus carrota). in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia
Belgrade: Serbian Plant Physiology Society., 36.
https://hdl.handle.net/21.15107/rcub_ibiss_6396
Selaković N, Jevremović S, Živković S, Maletić D, Puač N, Malović G, Petrović ZL. The effects of atmospheric pressure plasma on somatic embryogenesis  of carrot (Daucus carrota). in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia. 2013;:36.
https://hdl.handle.net/21.15107/rcub_ibiss_6396 .
Selaković, Nenad, Jevremović, Slađana, Živković, Suzana, Maletić, Dejan, Puač, Nevena, Malović, Gordana, Petrović, Zoran Lj, "The effects of atmospheric pressure plasma on somatic embryogenesis  of carrot (Daucus carrota)" in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia (2013):36,
https://hdl.handle.net/21.15107/rcub_ibiss_6396 .