TY  - JOUR
AU  - Sarac, Zorica
AU  - Aleksic, Jelena M.
AU  - Dodos, Tanja
AU  - Rajcevic, Nemanja
AU  - Bojović, Srđan
AU  - Marin, Petar D.
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2063
AB  - Due to the current lack of nuclear microsatellites (simple sequence
   repeats SSRs) specifically developed for Pinus nigra, an important
   European coniferous species, we cross-species amplified 12 EST-SSRs
   (expressed sequence tagged SSRs) developed for other Pinus species in P.
   nigra in order to delineate loci which can be used for assessing levels
   of genetic diversity and genetic structuring in this species. We
   amplified these loci in individuals from seven populations from the
   central Balkans representing four recognized infraspecific taxa of P.
   nigra (ssp. nigra, var. gocensis, ssp. pallasiana, and var. banatica).
   Contrary to expectations on high transferability of EST-SSRs into
   related species, only three out of 12 tested loci were successfully
   amplified in P. nigra, but they displayed lack/low levels of
   polymorphism or generated multilocus amplification products. Thus, our
   estimates on levels of genetic diversity (HE = 0.183) and genetic
   differentiation (F-ST = 0.007) were based on variability of a single
   locus harboring four alleles only and they should be taken with
   cautions. Our study highlights the need for the development of
   high-resolution molecular markers, such as co-dominant genic or genomic
   SSRs or predominantly biallelic SNPs, or utilization of anonymous
   dominant markers, such as AFLPs, for genotyping in P. nigra.
T2  - Genetika-Belgrade
T1  - CROSS-SPECIES AMPLIFICATION OF NUCLEAR EST-MICROSATELLITES DEVELOPED FOR
 OTHER Pinus SPECIES IN Pinus nigra
IS  - 1
VL  - 47
DO  - 10.2298/GENSR1501205S
SP  - 205
EP  - 217
ER  - 

@article{
author = "Sarac, Zorica and Aleksic, Jelena M. and Dodos, Tanja and Rajcevic, Nemanja and Bojović, Srđan and Marin, Petar D.",
year = "2015",
abstract = "Due to the current lack of nuclear microsatellites (simple sequence
   repeats SSRs) specifically developed for Pinus nigra, an important
   European coniferous species, we cross-species amplified 12 EST-SSRs
   (expressed sequence tagged SSRs) developed for other Pinus species in P.
   nigra in order to delineate loci which can be used for assessing levels
   of genetic diversity and genetic structuring in this species. We
   amplified these loci in individuals from seven populations from the
   central Balkans representing four recognized infraspecific taxa of P.
   nigra (ssp. nigra, var. gocensis, ssp. pallasiana, and var. banatica).
   Contrary to expectations on high transferability of EST-SSRs into
   related species, only three out of 12 tested loci were successfully
   amplified in P. nigra, but they displayed lack/low levels of
   polymorphism or generated multilocus amplification products. Thus, our
   estimates on levels of genetic diversity (HE = 0.183) and genetic
   differentiation (F-ST = 0.007) were based on variability of a single
   locus harboring four alleles only and they should be taken with
   cautions. Our study highlights the need for the development of
   high-resolution molecular markers, such as co-dominant genic or genomic
   SSRs or predominantly biallelic SNPs, or utilization of anonymous
   dominant markers, such as AFLPs, for genotyping in P. nigra.",
journal = "Genetika-Belgrade",
title = "CROSS-SPECIES AMPLIFICATION OF NUCLEAR EST-MICROSATELLITES DEVELOPED FOR
 OTHER Pinus SPECIES IN Pinus nigra",
number = "1",
volume = "47",
doi = "10.2298/GENSR1501205S",
pages = "205-217"
}

Sarac, Z., Aleksic, J. M., Dodos, T., Rajcevic, N., Bojović, S.,& Marin, P. D.. (2015). CROSS-SPECIES AMPLIFICATION OF NUCLEAR EST-MICROSATELLITES DEVELOPED FOR
 OTHER Pinus SPECIES IN Pinus nigra. in Genetika-Belgrade, 47(1), 205-217.
https://doi.org/10.2298/GENSR1501205S

Sarac Z, Aleksic JM, Dodos T, Rajcevic N, Bojović S, Marin PD. CROSS-SPECIES AMPLIFICATION OF NUCLEAR EST-MICROSATELLITES DEVELOPED FOR
 OTHER Pinus SPECIES IN Pinus nigra. in Genetika-Belgrade. 2015;47(1):205-217.
doi:10.2298/GENSR1501205S .

Sarac, Zorica, Aleksic, Jelena M., Dodos, Tanja, Rajcevic, Nemanja, Bojović, Srđan, Marin, Petar D., "CROSS-SPECIES AMPLIFICATION OF NUCLEAR EST-MICROSATELLITES DEVELOPED FOR
 OTHER Pinus SPECIES IN Pinus nigra" in Genetika-Belgrade, 47, no. 1 (2015):205-217,
https://doi.org/10.2298/GENSR1501205S . .

TY  - JOUR
AU  - Šarac, Zorica
AU  - Dodos, Tanja
AU  - Rajčević, Nemanja
AU  - Bojović, Srđan
AU  - Marin, Petar D.
AU  - Aleksić, Jelena M.
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2040
AB  - Pinus nigra J.F. Arnold, European black pine, is a typical component of
   Mediterranean and sub-Mediterranean coniferous forests with highly
   fragmentary distribution. Western Mediterranean populations of this
   species have been studied genetically to date, while eastern populations
   from the central Balkans, which are larger and more abundant, are still
   genetically understudied. We analyzed seven populations of P. nigra
   representing all infraspecific taxa recognized within the central
   Balkans (subspecies nigra with varieties nigra and gocensis Dordevic;
   and subspecies pallasiana (Lamb.) Holmboe with varieties pallasiana and
   banatica (Endl.) Georgescu et Ionescu), with three chloroplast
   microsatellites (cpDNA SSRs) and one mitochondrial (mtDNA) locus.
   Although our molecular data failed to support circumscription of studied
   infraspecific taxa, we found that genetic patterns at both genomes are
   in accordance with those found previously in westward populations of
   this species, that is - exceptionally high levels of genetic diversity
   (H-T = 0.949) and low genetic differentiation (G(ST) = 0.024) at the
   cpDNA level, and moderate levels of genetic diversity (H-T = 0.357) and
   genetic differentiation (G(ST) = 0.358) at the mtDNA level. Based on
   genealogical relations of mtDNA types currently present in Balkans' and
   Iberian/African populations, we inferred that the ancestral gene pool of
   P. nigra already harbored polymorphism at position 328 prior to the
   divergence to two lineages currently present in westward and eastward
   parts of the species range distribution. Subsequent occurrence of three
   mutations, which distinguish these two lineages, suggests their
   long-term isolation.
T2  - Silva Fennica
T1  - Genetic patterns in Pinus nigra from the central Balkans inferred from
 plastid and mitochondrial data
IS  - 1415
VL  - 49
DO  - 10.14214/sf.1415
ER  - 

@article{
author = "Šarac, Zorica and Dodos, Tanja and Rajčević, Nemanja and Bojović, Srđan and Marin, Petar D. and Aleksić, Jelena M.",
year = "2015",
abstract = "Pinus nigra J.F. Arnold, European black pine, is a typical component of
   Mediterranean and sub-Mediterranean coniferous forests with highly
   fragmentary distribution. Western Mediterranean populations of this
   species have been studied genetically to date, while eastern populations
   from the central Balkans, which are larger and more abundant, are still
   genetically understudied. We analyzed seven populations of P. nigra
   representing all infraspecific taxa recognized within the central
   Balkans (subspecies nigra with varieties nigra and gocensis Dordevic;
   and subspecies pallasiana (Lamb.) Holmboe with varieties pallasiana and
   banatica (Endl.) Georgescu et Ionescu), with three chloroplast
   microsatellites (cpDNA SSRs) and one mitochondrial (mtDNA) locus.
   Although our molecular data failed to support circumscription of studied
   infraspecific taxa, we found that genetic patterns at both genomes are
   in accordance with those found previously in westward populations of
   this species, that is - exceptionally high levels of genetic diversity
   (H-T = 0.949) and low genetic differentiation (G(ST) = 0.024) at the
   cpDNA level, and moderate levels of genetic diversity (H-T = 0.357) and
   genetic differentiation (G(ST) = 0.358) at the mtDNA level. Based on
   genealogical relations of mtDNA types currently present in Balkans' and
   Iberian/African populations, we inferred that the ancestral gene pool of
   P. nigra already harbored polymorphism at position 328 prior to the
   divergence to two lineages currently present in westward and eastward
   parts of the species range distribution. Subsequent occurrence of three
   mutations, which distinguish these two lineages, suggests their
   long-term isolation.",
journal = "Silva Fennica",
title = "Genetic patterns in Pinus nigra from the central Balkans inferred from
 plastid and mitochondrial data",
number = "1415",
volume = "49",
doi = "10.14214/sf.1415"
}

Šarac, Z., Dodos, T., Rajčević, N., Bojović, S., Marin, P. D.,& Aleksić, J. M.. (2015). Genetic patterns in Pinus nigra from the central Balkans inferred from
 plastid and mitochondrial data. in Silva Fennica, 49(1415).
https://doi.org/10.14214/sf.1415

Šarac Z, Dodos T, Rajčević N, Bojović S, Marin PD, Aleksić JM. Genetic patterns in Pinus nigra from the central Balkans inferred from
 plastid and mitochondrial data. in Silva Fennica. 2015;49(1415).
doi:10.14214/sf.1415 .

Šarac, Zorica, Dodos, Tanja, Rajčević, Nemanja, Bojović, Srđan, Marin, Petar D., Aleksić, Jelena M., "Genetic patterns in Pinus nigra from the central Balkans inferred from
 plastid and mitochondrial data" in Silva Fennica, 49, no. 1415 (2015),
https://doi.org/10.14214/sf.1415 . .

TY  - JOUR
AU  - Rajcevic, Nemanja
AU  - Janackovic, Pedja
AU  - Dodos, Tanja
AU  - Tesevic, Vele
AU  - Bojović, Srđan
AU  - Marin, Petar D.
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2191
AB  - The composition of the cuticular n-alkanes isolated from the leaves of
   nine populations of Juniperus deltoides R. P. Adams from continental and
   coastal areas of the Balkan Peninsula was characterized by GC-FID and
   GC/MS analyses. In the leaf waxes, 14 n-alkane homologues with
   chain-lengths ranging from C22 to C35 were identified. n-Tritriacontane
   (C33) was dominant in the waxes of all populations, but variations
   between the populations in the contents of all n-alkanes were observed.
   Several statistical methods (ANOVA, principal component, discriminant,
   and cluster analyses) were used to investigate the diversity and
   variability of the cuticular-leaf-n-alkane patterns of the nine J.
   deltoides populations. This is the first report on the n-alkane
   composition for this species. The multivariate statistical analyses
   evidenced a high correlation of the leaf-n-alkane pattern with the
   geographical distribution of the investigated samples, differentiating
   the coastal from the continental populations of this taxon.
T2  - Chemistry & Biodiversity
T1  - Leaf n-Alkanes as Characters Differentiating Coastal and Continental
 Juniperus deltoides Populations from the Balkan Peninsula
IS  - 7
VL  - 11
SP  - 1042
EP  - 1052
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_2191
ER  - 

@article{
author = "Rajcevic, Nemanja and Janackovic, Pedja and Dodos, Tanja and Tesevic, Vele and Bojović, Srđan and Marin, Petar D.",
year = "2014",
abstract = "The composition of the cuticular n-alkanes isolated from the leaves of
   nine populations of Juniperus deltoides R. P. Adams from continental and
   coastal areas of the Balkan Peninsula was characterized by GC-FID and
   GC/MS analyses. In the leaf waxes, 14 n-alkane homologues with
   chain-lengths ranging from C22 to C35 were identified. n-Tritriacontane
   (C33) was dominant in the waxes of all populations, but variations
   between the populations in the contents of all n-alkanes were observed.
   Several statistical methods (ANOVA, principal component, discriminant,
   and cluster analyses) were used to investigate the diversity and
   variability of the cuticular-leaf-n-alkane patterns of the nine J.
   deltoides populations. This is the first report on the n-alkane
   composition for this species. The multivariate statistical analyses
   evidenced a high correlation of the leaf-n-alkane pattern with the
   geographical distribution of the investigated samples, differentiating
   the coastal from the continental populations of this taxon.",
journal = "Chemistry & Biodiversity",
title = "Leaf n-Alkanes as Characters Differentiating Coastal and Continental
 Juniperus deltoides Populations from the Balkan Peninsula",
number = "7",
volume = "11",
pages = "1042-1052",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_2191"
}

Rajcevic, N., Janackovic, P., Dodos, T., Tesevic, V., Bojović, S.,& Marin, P. D.. (2014). Leaf n-Alkanes as Characters Differentiating Coastal and Continental
 Juniperus deltoides Populations from the Balkan Peninsula. in Chemistry & Biodiversity, 11(7), 1042-1052.
https://hdl.handle.net/21.15107/rcub_ibiss_2191

Rajcevic N, Janackovic P, Dodos T, Tesevic V, Bojović S, Marin PD. Leaf n-Alkanes as Characters Differentiating Coastal and Continental
 Juniperus deltoides Populations from the Balkan Peninsula. in Chemistry & Biodiversity. 2014;11(7):1042-1052.
https://hdl.handle.net/21.15107/rcub_ibiss_2191 .

Rajcevic, Nemanja, Janackovic, Pedja, Dodos, Tanja, Tesevic, Vele, Bojović, Srđan, Marin, Petar D., "Leaf n-Alkanes as Characters Differentiating Coastal and Continental
 Juniperus deltoides Populations from the Balkan Peninsula" in Chemistry & Biodiversity, 11, no. 7 (2014):1042-1052,
https://hdl.handle.net/21.15107/rcub_ibiss_2191 .