TY  - JOUR
AU  - Hmaid, Amal AAA
AU  - Markelić, Milica
AU  - Otašević, Vesna
AU  - Mašović, Sava
AU  - Janković, Aleksandra
AU  - Korać, Bato
AU  - Korać, Aleksandra
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6212
AB  - Structural changes affecting cardiomyocyte function may contribute to the pathophysiological remodeling underlying cardiac function impairment. Recent reports have shown that endogenous nitric oxide (NO) plays an important role in this process. In order to examine the role of NO in cardiomyocyte remodeling, male rats were acclimated to room temperature (22 ± 1 °C) or cold (4 ± 1 °C) and treated with 2.25% l-arginine·HCl or 0.01% l-NAME (Nω-nitro-l-arginine methyl ester)·HCl for 45 days. Untreated groups served as controls. Right heart ventricles were routinely prepared for light microscopic examination. Stereological estimations of volume densities of cardiomyocytes, surrounding blood vessels and connective tissue, as well as the morphometric measurements of cardiomyocyte diameters were performed. Tissue sections were also analyzed for structural alterations. We observed that both l-arginine and l-NAME supplementation induced cardiomyocyte hypertrophy, regardless of ambient temperature. However, cardiomyocyte hypertrophy was associated with fibrosis and extra collagen deposition only in the l-NAME treated group. Taken together, our results suggest that NO has a modulatory role in right heart ventricle remodeling by coordinating hypertrophy of cardiomyocytes and fibrous tissue preventing cardiac fibrosis.
PB  - Elsevier
T2  - Saudi Journal of Biological Sciences
T1  - Structural alterations in rat myocardium induced by chronic l-arginine and l-NAME supplementation
IS  - 3
VL  - 25
DO  - 10.1016/j.sjbs.2016.01.022
SP  - 537
EP  - 544
ER  - 

@article{
author = "Hmaid, Amal AAA and Markelić, Milica and Otašević, Vesna and Mašović, Sava and Janković, Aleksandra and Korać, Bato and Korać, Aleksandra",
year = "2016",
abstract = "Structural changes affecting cardiomyocyte function may contribute to the pathophysiological remodeling underlying cardiac function impairment. Recent reports have shown that endogenous nitric oxide (NO) plays an important role in this process. In order to examine the role of NO in cardiomyocyte remodeling, male rats were acclimated to room temperature (22 ± 1 °C) or cold (4 ± 1 °C) and treated with 2.25% l-arginine·HCl or 0.01% l-NAME (Nω-nitro-l-arginine methyl ester)·HCl for 45 days. Untreated groups served as controls. Right heart ventricles were routinely prepared for light microscopic examination. Stereological estimations of volume densities of cardiomyocytes, surrounding blood vessels and connective tissue, as well as the morphometric measurements of cardiomyocyte diameters were performed. Tissue sections were also analyzed for structural alterations. We observed that both l-arginine and l-NAME supplementation induced cardiomyocyte hypertrophy, regardless of ambient temperature. However, cardiomyocyte hypertrophy was associated with fibrosis and extra collagen deposition only in the l-NAME treated group. Taken together, our results suggest that NO has a modulatory role in right heart ventricle remodeling by coordinating hypertrophy of cardiomyocytes and fibrous tissue preventing cardiac fibrosis.",
publisher = "Elsevier",
journal = "Saudi Journal of Biological Sciences",
title = "Structural alterations in rat myocardium induced by chronic l-arginine and l-NAME supplementation",
number = "3",
volume = "25",
doi = "10.1016/j.sjbs.2016.01.022",
pages = "537-544"
}

Hmaid, A. A., Markelić, M., Otašević, V., Mašović, S., Janković, A., Korać, B.,& Korać, A.. (2016). Structural alterations in rat myocardium induced by chronic l-arginine and l-NAME supplementation. in Saudi Journal of Biological Sciences
Elsevier., 25(3), 537-544.
https://doi.org/10.1016/j.sjbs.2016.01.022

Hmaid AA, Markelić M, Otašević V, Mašović S, Janković A, Korać B, Korać A. Structural alterations in rat myocardium induced by chronic l-arginine and l-NAME supplementation. in Saudi Journal of Biological Sciences. 2016;25(3):537-544.
doi:10.1016/j.sjbs.2016.01.022 .

Hmaid, Amal AAA, Markelić, Milica, Otašević, Vesna, Mašović, Sava, Janković, Aleksandra, Korać, Bato, Korać, Aleksandra, "Structural alterations in rat myocardium induced by chronic l-arginine and l-NAME supplementation" in Saudi Journal of Biological Sciences, 25, no. 3 (2016):537-544,
https://doi.org/10.1016/j.sjbs.2016.01.022 . .