TY  - CONF
AU  - Predojević, Dragana
AU  - Savković, Uroš
AU  - Đorđević, Mirko
AU  - Vlajnić, Lea
AU  - Stojković, Biljana
AU  - Pešić, Snežana
AU  - Vukajlović, Filip
AU  - Mitrovski Bogdanović, Ana
AU  - Stojković, Oliver
AU  - Budečević, Sanja
PY  - 2023
UR  - https://ece2023.com/scientific-program/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6253
AB  - Insecticide resistance is an example of rapid evolution under strong selection pressure and, is therefore of great importance for human and environmental health and economy. Acanthoscelides obtectus is a globally distributed Coleoptera pest that attacks bean seeds and degrades their quality. One method of protecting against seed beetles is the application of a widely used group of pesticides- pyrethroids. Insecticides, as xenobiotics, can induce stress in pests, leading to destabilization of their development and changes in their morphology. Fluctuating asymmetry (FA), small random deviations between right and left sides of bilateral symmetrical traits, is considered as a result of developmental instability and is thus a measurable indicator of phenotypic response to stress during morphogenesis in insects. In this work, we exposed four populations of A. obtectus to sub-lethal doses (LD20) of pyrethroid insecticide for five generations in a laboratory evolution experiment. To estimate the level of stress, we monitored the effects of the pesticide on shape changes and the level of FA using a geometric morphometric approach. Our results showed that beetles treated with LD20 significantly changed their morphology in the thorax region, while the effect on the level of FA was minor. This work uses experimental evolution as a powerful tool to study pesticide resistance and paves the way for further studies on morphological adaptations, modularity and developmental stability under stress conditions.
PB  - Hellenic Entomological Society
C3  - Book of abstracts: 12th European Congress of Entomology: ECE 2023 Crete; 2023 Oct 16-20; Heraklion, Greece
T1  - Evolution of pesticide resistance in seed beetles: transgenerational effects of sub-lethal pyrethroid concentration on morphology and development
SP  - 365
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6253
ER  - 

@conference{
author = "Predojević, Dragana and Savković, Uroš and Đorđević, Mirko and Vlajnić, Lea and Stojković, Biljana and Pešić, Snežana and Vukajlović, Filip and Mitrovski Bogdanović, Ana and Stojković, Oliver and Budečević, Sanja",
year = "2023",
abstract = "Insecticide resistance is an example of rapid evolution under strong selection pressure and, is therefore of great importance for human and environmental health and economy. Acanthoscelides obtectus is a globally distributed Coleoptera pest that attacks bean seeds and degrades their quality. One method of protecting against seed beetles is the application of a widely used group of pesticides- pyrethroids. Insecticides, as xenobiotics, can induce stress in pests, leading to destabilization of their development and changes in their morphology. Fluctuating asymmetry (FA), small random deviations between right and left sides of bilateral symmetrical traits, is considered as a result of developmental instability and is thus a measurable indicator of phenotypic response to stress during morphogenesis in insects. In this work, we exposed four populations of A. obtectus to sub-lethal doses (LD20) of pyrethroid insecticide for five generations in a laboratory evolution experiment. To estimate the level of stress, we monitored the effects of the pesticide on shape changes and the level of FA using a geometric morphometric approach. Our results showed that beetles treated with LD20 significantly changed their morphology in the thorax region, while the effect on the level of FA was minor. This work uses experimental evolution as a powerful tool to study pesticide resistance and paves the way for further studies on morphological adaptations, modularity and developmental stability under stress conditions.",
publisher = "Hellenic Entomological Society",
journal = "Book of abstracts: 12th European Congress of Entomology: ECE 2023 Crete; 2023 Oct 16-20; Heraklion, Greece",
title = "Evolution of pesticide resistance in seed beetles: transgenerational effects of sub-lethal pyrethroid concentration on morphology and development",
pages = "365",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6253"
}

Predojević, D., Savković, U., Đorđević, M., Vlajnić, L., Stojković, B., Pešić, S., Vukajlović, F., Mitrovski Bogdanović, A., Stojković, O.,& Budečević, S.. (2023). Evolution of pesticide resistance in seed beetles: transgenerational effects of sub-lethal pyrethroid concentration on morphology and development. in Book of abstracts: 12th European Congress of Entomology: ECE 2023 Crete; 2023 Oct 16-20; Heraklion, Greece
Hellenic Entomological Society., 365.
https://hdl.handle.net/21.15107/rcub_ibiss_6253

Predojević D, Savković U, Đorđević M, Vlajnić L, Stojković B, Pešić S, Vukajlović F, Mitrovski Bogdanović A, Stojković O, Budečević S. Evolution of pesticide resistance in seed beetles: transgenerational effects of sub-lethal pyrethroid concentration on morphology and development. in Book of abstracts: 12th European Congress of Entomology: ECE 2023 Crete; 2023 Oct 16-20; Heraklion, Greece. 2023;:365.
https://hdl.handle.net/21.15107/rcub_ibiss_6253 .

Predojević, Dragana, Savković, Uroš, Đorđević, Mirko, Vlajnić, Lea, Stojković, Biljana, Pešić, Snežana, Vukajlović, Filip, Mitrovski Bogdanović, Ana, Stojković, Oliver, Budečević, Sanja, "Evolution of pesticide resistance in seed beetles: transgenerational effects of sub-lethal pyrethroid concentration on morphology and development" in Book of abstracts: 12th European Congress of Entomology: ECE 2023 Crete; 2023 Oct 16-20; Heraklion, Greece (2023):365,
https://hdl.handle.net/21.15107/rcub_ibiss_6253 .

TY  - DATA
AU  - Vlajnić, Lea
AU  - Savković, Uroš
AU  - Vukajlović, Filip
AU  - Budečević, Sanja
AU  - Stojković, Biljana
AU  - Stojković, Oliver
AU  - Pešić, Snežana
AU  - Predojević, Dragana
AU  - Mitrovski Bogdanović, Ana
AU  - Đorđević, Mirko
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6018
AB  - This data set is a milestone of the WP3 work package of the Experimental evolution approach in developing insect pest control methods (ELEVATE) project number #7683961 financed by the Science Fund of the Republic of Serbia. Data set contains sequences of subunit 1 of cytochrome oxidase (COI) for populations of seed beetles (Acanthoscelides obtectus) with different shares of Trojan female technique (TFT) and control mytotypes.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
T1  - Milestone for the ELEVATE project: Data on changes in genetic structure of populations relative to starting frequencies of TFT females
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6018
ER  - 

@misc{
author = "Vlajnić, Lea and Savković, Uroš and Vukajlović, Filip and Budečević, Sanja and Stojković, Biljana and Stojković, Oliver and Pešić, Snežana and Predojević, Dragana and Mitrovski Bogdanović, Ana and Đorđević, Mirko",
year = "2023",
abstract = "This data set is a milestone of the WP3 work package of the Experimental evolution approach in developing insect pest control methods (ELEVATE) project number #7683961 financed by the Science Fund of the Republic of Serbia. Data set contains sequences of subunit 1 of cytochrome oxidase (COI) for populations of seed beetles (Acanthoscelides obtectus) with different shares of Trojan female technique (TFT) and control mytotypes.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
title = "Milestone for the ELEVATE project: Data on changes in genetic structure of populations relative to starting frequencies of TFT females",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6018"
}

Vlajnić, L., Savković, U., Vukajlović, F., Budečević, S., Stojković, B., Stojković, O., Pešić, S., Predojević, D., Mitrovski Bogdanović, A.,& Đorđević, M.. (2023). Milestone for the ELEVATE project: Data on changes in genetic structure of populations relative to starting frequencies of TFT females. 
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade..
https://hdl.handle.net/21.15107/rcub_ibiss_6018

Vlajnić L, Savković U, Vukajlović F, Budečević S, Stojković B, Stojković O, Pešić S, Predojević D, Mitrovski Bogdanović A, Đorđević M. Milestone for the ELEVATE project: Data on changes in genetic structure of populations relative to starting frequencies of TFT females. 2023;.
https://hdl.handle.net/21.15107/rcub_ibiss_6018 .

Vlajnić, Lea, Savković, Uroš, Vukajlović, Filip, Budečević, Sanja, Stojković, Biljana, Stojković, Oliver, Pešić, Snežana, Predojević, Dragana, Mitrovski Bogdanović, Ana, Đorđević, Mirko, "Milestone for the ELEVATE project: Data on changes in genetic structure of populations relative to starting frequencies of TFT females" (2023),
https://hdl.handle.net/21.15107/rcub_ibiss_6018 .

TY  - CONF
AU  - Savković, Uroš
AU  - Đorđević, Mirko
AU  - Budečević, Sanja
AU  - Vlajnić, Lea
AU  - Pešić, Snežana
AU  - Vukajlović, Filip
AU  - Predojević, Dragana
AU  - Mitrovski Bogdanović, Ana
AU  - Stojković, Oliver
AU  - Stojković, Biljana
PY  - 2022
UR  - https://www.eseb2022.cz/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5030
AB  - Diverse aspects of insects’ behaviour, physiology, and the relationship between life-history traits are challenged when insects try to expand their host range, exploit alternative food sources and specialise on them. Process that enables phytophagous insects to utilise new food sources, known as host shift, is tightly associated with developmental plasticity and is seldom studied in laboratory settings. Using an experimental evolution approach we simulated the host shift process and observed the evolution of plasticity in seed beetle (Acanthoscelides obtectus) laboratory populations that evolved on optimal (common beans) and suboptimal (chickpea) plant hosts for more than 35 years. We have looked into: 1) life-history traits and how the long-term exposure to different hosts affects them; 2) the consequences when insects are exposed to short-term (in a single generation) change of the host plant, and 3) what happens when the host plant is altered each generation, that is, we observed  the process of the selection for increased plasticity in a laboratory setting. Prior to life-history assays, populations were in the experiment for 13 generations. We found that long-term host shift to chickpeas decreased plasticity levels for preadult traits compared to bean adapted populations. Simultaneously, fecundity evolved a more plastic response. Groups that were evolving in conditions where plant hosts were alternated each generation had the same plasticity patterns as their ancestral populations, suggesting the need for more time for plastic response to evolve. This research illustrates the importance of phenotypic plasticity in maintaining populations under changing feeding conditions.
PB  - European Society for Evolutionary Biology
C3  - Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic
T1  - Developmental plasticity and the potential of host shift in the seed beetle
SP  - 184
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5030
ER  - 

@conference{
author = "Savković, Uroš and Đorđević, Mirko and Budečević, Sanja and Vlajnić, Lea and Pešić, Snežana and Vukajlović, Filip and Predojević, Dragana and Mitrovski Bogdanović, Ana and Stojković, Oliver and Stojković, Biljana",
year = "2022",
abstract = "Diverse aspects of insects’ behaviour, physiology, and the relationship between life-history traits are challenged when insects try to expand their host range, exploit alternative food sources and specialise on them. Process that enables phytophagous insects to utilise new food sources, known as host shift, is tightly associated with developmental plasticity and is seldom studied in laboratory settings. Using an experimental evolution approach we simulated the host shift process and observed the evolution of plasticity in seed beetle (Acanthoscelides obtectus) laboratory populations that evolved on optimal (common beans) and suboptimal (chickpea) plant hosts for more than 35 years. We have looked into: 1) life-history traits and how the long-term exposure to different hosts affects them; 2) the consequences when insects are exposed to short-term (in a single generation) change of the host plant, and 3) what happens when the host plant is altered each generation, that is, we observed  the process of the selection for increased plasticity in a laboratory setting. Prior to life-history assays, populations were in the experiment for 13 generations. We found that long-term host shift to chickpeas decreased plasticity levels for preadult traits compared to bean adapted populations. Simultaneously, fecundity evolved a more plastic response. Groups that were evolving in conditions where plant hosts were alternated each generation had the same plasticity patterns as their ancestral populations, suggesting the need for more time for plastic response to evolve. This research illustrates the importance of phenotypic plasticity in maintaining populations under changing feeding conditions.",
publisher = "European Society for Evolutionary Biology",
journal = "Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic",
title = "Developmental plasticity and the potential of host shift in the seed beetle",
pages = "184",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5030"
}

Savković, U., Đorđević, M., Budečević, S., Vlajnić, L., Pešić, S., Vukajlović, F., Predojević, D., Mitrovski Bogdanović, A., Stojković, O.,& Stojković, B.. (2022). Developmental plasticity and the potential of host shift in the seed beetle. in Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic
European Society for Evolutionary Biology., 184.
https://hdl.handle.net/21.15107/rcub_ibiss_5030

Savković U, Đorđević M, Budečević S, Vlajnić L, Pešić S, Vukajlović F, Predojević D, Mitrovski Bogdanović A, Stojković O, Stojković B. Developmental plasticity and the potential of host shift in the seed beetle. in Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic. 2022;:184.
https://hdl.handle.net/21.15107/rcub_ibiss_5030 .

Savković, Uroš, Đorđević, Mirko, Budečević, Sanja, Vlajnić, Lea, Pešić, Snežana, Vukajlović, Filip, Predojević, Dragana, Mitrovski Bogdanović, Ana, Stojković, Oliver, Stojković, Biljana, "Developmental plasticity and the potential of host shift in the seed beetle" in Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic (2022):184,
https://hdl.handle.net/21.15107/rcub_ibiss_5030 .