TY  - CONF
AU  - Nikolić, Ljiljana
AU  - Korać, Jelena
AU  - Bijelić, Dunja
AU  - Spasojević, Ivan
AU  - Bogdanović Pristov, Jelena
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5510
AB  - Iron, an essential element for living organisms, participates in a wide range of metabolic
processes. It appears predominantly firmly bound to proteins, but can also be loosely bound to
low-affinity ligands, referred as labile iron pool (LIP). The composition and amount of LIP can
vary considerably under different physiological conditions, playing a beneficial role in iron
economy and homeostasis or contributing to the generation of reactive oxygen species. It is
still not known if bioactivity of low-affinity ligands can be modulated by iron binding.
Catecholamine neurotransmitters including norepinephrine (NE) can chelate iron. In the close
vicinity of synaptic cleft, astrocytes are direct target of norepinephrine. Here we show on
cultured rat cortical astrocytes that iron bound to NE completely blocks neurotransmitter
activity of NE. However, how astrocyte activity changes when norepinephrine binds iron
remains unknown.
We show, using spectrophotometry that NE and Fe3+ form complex in the 1:1 stoichiometry,
at pH 7.4. Iron effect on astrocyte response to NE was examined by the whole-cell patch-clamp
technique. NE alone evokes changes in the membrane currents of astrocytes, but such effects
were not observed for the NE- Fe3+ complex.
Our results demonstrating that iron in the complex with norepinephrine inhibits alpha-adrenergic
receptors and modulates astrocyte activity, imply a novel neuromodulatory role for LIP.
PB  - COST Action CA15133
C3  - Book of abstracts: 4th FeSBioNet Meeting: COST Action CA15133; 2019 Sep 16-19; Gdansk, Poland
T1  - Iron modulates norepinephrine effect on astrocytes
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5510
ER  - 

@conference{
author = "Nikolić, Ljiljana and Korać, Jelena and Bijelić, Dunja and Spasojević, Ivan and Bogdanović Pristov, Jelena",
year = "2019",
abstract = "Iron, an essential element for living organisms, participates in a wide range of metabolic
processes. It appears predominantly firmly bound to proteins, but can also be loosely bound to
low-affinity ligands, referred as labile iron pool (LIP). The composition and amount of LIP can
vary considerably under different physiological conditions, playing a beneficial role in iron
economy and homeostasis or contributing to the generation of reactive oxygen species. It is
still not known if bioactivity of low-affinity ligands can be modulated by iron binding.
Catecholamine neurotransmitters including norepinephrine (NE) can chelate iron. In the close
vicinity of synaptic cleft, astrocytes are direct target of norepinephrine. Here we show on
cultured rat cortical astrocytes that iron bound to NE completely blocks neurotransmitter
activity of NE. However, how astrocyte activity changes when norepinephrine binds iron
remains unknown.
We show, using spectrophotometry that NE and Fe3+ form complex in the 1:1 stoichiometry,
at pH 7.4. Iron effect on astrocyte response to NE was examined by the whole-cell patch-clamp
technique. NE alone evokes changes in the membrane currents of astrocytes, but such effects
were not observed for the NE- Fe3+ complex.
Our results demonstrating that iron in the complex with norepinephrine inhibits alpha-adrenergic
receptors and modulates astrocyte activity, imply a novel neuromodulatory role for LIP.",
publisher = "COST Action CA15133",
journal = "Book of abstracts: 4th FeSBioNet Meeting: COST Action CA15133; 2019 Sep 16-19; Gdansk, Poland",
title = "Iron modulates norepinephrine effect on astrocytes",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5510"
}

Nikolić, L., Korać, J., Bijelić, D., Spasojević, I.,& Bogdanović Pristov, J.. (2019). Iron modulates norepinephrine effect on astrocytes. in Book of abstracts: 4th FeSBioNet Meeting: COST Action CA15133; 2019 Sep 16-19; Gdansk, Poland
COST Action CA15133..
https://hdl.handle.net/21.15107/rcub_ibiss_5510

Nikolić L, Korać J, Bijelić D, Spasojević I, Bogdanović Pristov J. Iron modulates norepinephrine effect on astrocytes. in Book of abstracts: 4th FeSBioNet Meeting: COST Action CA15133; 2019 Sep 16-19; Gdansk, Poland. 2019;.
https://hdl.handle.net/21.15107/rcub_ibiss_5510 .

Nikolić, Ljiljana, Korać, Jelena, Bijelić, Dunja, Spasojević, Ivan, Bogdanović Pristov, Jelena, "Iron modulates norepinephrine effect on astrocytes" in Book of abstracts: 4th FeSBioNet Meeting: COST Action CA15133; 2019 Sep 16-19; Gdansk, Poland (2019),
https://hdl.handle.net/21.15107/rcub_ibiss_5510 .

TY  - CONF
AU  - Korać, Jelena
AU  - Nikolić, Ljiljana
AU  - Bijelić, Dunja
AU  - Spasojević, Ivan
AU  - Bogdanović Pristov, Jelena
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5501
AB  - Aims: Astrocyte position between synapses and blood vessels allows them to ful l crucial functions such as regulation of
synaptic activity and potassium bu ering. Well positioned in the close vicinity of synaptic cleft astrocytes are considered to be
a direct target of norepinephrine (NE). Synaptic activity and neurotransmitter actions can be in uenced by extracellular iron.
Here we investigated whether iron interacts with NE and if this interaction can modulate astrocyte response to NE.
Methods: To investigate the interaction between iron and norepinephrine we used spectrophotometry approach. Iron e ect
on astrocyte response to NE was examined by the whole-cell patch-clamp technique. Membrane currents were recorded from
cultured cortical astrocytes prepared from WT rats.
Results: Using spectrophotometry we observed that iron interacts with NE which leads to the formation of a stable complex
in the 1:1 stoichiometry. We also found that iron bound to NE completely blocks NE-induced increase of large-conductance
calcium sensitive potassium current in astrocytes.
Conclusions: Astrocyte response to NE is modi ed when this neurotransmitter forms a complex with iron. This implies that
NE binding to astrocytic noradrenergic receptors may be prevented by iron. Our  ndings point toward compromised astrocyte
functions related to the potassium bu ering when NE action is modi ed by iron.
PB  - Belgrade : Serbian Neuroscience Society
C3  - Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
T1  - Iron modulates norepinephrine effect on astrocytes
SP  - 370
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5501
ER  - 

@conference{
author = "Korać, Jelena and Nikolić, Ljiljana and Bijelić, Dunja and Spasojević, Ivan and Bogdanović Pristov, Jelena",
year = "2019",
abstract = "Aims: Astrocyte position between synapses and blood vessels allows them to ful l crucial functions such as regulation of
synaptic activity and potassium bu ering. Well positioned in the close vicinity of synaptic cleft astrocytes are considered to be
a direct target of norepinephrine (NE). Synaptic activity and neurotransmitter actions can be in uenced by extracellular iron.
Here we investigated whether iron interacts with NE and if this interaction can modulate astrocyte response to NE.
Methods: To investigate the interaction between iron and norepinephrine we used spectrophotometry approach. Iron e ect
on astrocyte response to NE was examined by the whole-cell patch-clamp technique. Membrane currents were recorded from
cultured cortical astrocytes prepared from WT rats.
Results: Using spectrophotometry we observed that iron interacts with NE which leads to the formation of a stable complex
in the 1:1 stoichiometry. We also found that iron bound to NE completely blocks NE-induced increase of large-conductance
calcium sensitive potassium current in astrocytes.
Conclusions: Astrocyte response to NE is modi ed when this neurotransmitter forms a complex with iron. This implies that
NE binding to astrocytic noradrenergic receptors may be prevented by iron. Our  ndings point toward compromised astrocyte
functions related to the potassium bu ering when NE action is modi ed by iron.",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia",
title = "Iron modulates norepinephrine effect on astrocytes",
pages = "370",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5501"
}

Korać, J., Nikolić, L., Bijelić, D., Spasojević, I.,& Bogdanović Pristov, J.. (2019). Iron modulates norepinephrine effect on astrocytes. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
Belgrade : Serbian Neuroscience Society., 370.
https://hdl.handle.net/21.15107/rcub_ibiss_5501

Korać J, Nikolić L, Bijelić D, Spasojević I, Bogdanović Pristov J. Iron modulates norepinephrine effect on astrocytes. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia. 2019;:370.
https://hdl.handle.net/21.15107/rcub_ibiss_5501 .

Korać, Jelena, Nikolić, Ljiljana, Bijelić, Dunja, Spasojević, Ivan, Bogdanović Pristov, Jelena, "Iron modulates norepinephrine effect on astrocytes" in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia (2019):370,
https://hdl.handle.net/21.15107/rcub_ibiss_5501 .

TY  - CONF
AU  - Korać, Jelena
AU  - Stanković, Dalibor
AU  - Bogdanović Pristov, Jelena
AU  - Nikolić, Ljiljana
AU  - Spasojević, Ivan
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5517
AB  - Epinephrine ((R)-4-(1-hydroxy-2-(methylamino)ethyl)-benzene-1,2-diol (Epi) is
catecholamine that is released by the sympathetic nervous system and adrenal medulla. It
is a physiologically important molecule that acts as a hormone, neurotransmitter, and
medication with a broad range of effects 1-3 . Coordinate and redox interaction of Epi with
iron affects the interactions with other molecules and its biological effects 4
. In this study,
we reported details of redox interactions of Epi with Fe 2+ at pH 7.4, which correspond to
the pH value of human plasma Epi and Fe 2+ form a complex that acts as a strong reducing
agent. Cyclic voltammetry showed that the positions of E pa and E pc potentials were at
approximately -480 and -1100 mV. This implies that Epi and Fe 2+ build a complex with
unique redox properties. E1/2 was significantly lower compared to E0' for O 2 /O 2•- (-350
mV). It is important to point out this because superoxide radical anion is produced via
spontaneous Fe 2+ reaction with O 2. In other words, Epi-Fe 2+ complex should be capable of
reducing transition metals in (patho)physiologicaly relevant complexes that are not
susceptible to reduction by O 2. Our results confirmed that Epi-Fe
2+ is capable of reducing
the S-S group of glutathione disulfide. On the other hand, Epi acted in a catalyst-like
fashion to promote Fe 2+ oxidation by molecular oxygen, and to a facilitated formation of
the Epi–Fe 3+ complexes, at physiological pH. In addition, we examined the effects of
epinepfrine and Epi/Fe3+ system on glioma cells. Epinephrine alone evokes changes in the
membrane currents of glioma cells, but such effects were not observed for the complex
with Fe 3+
. This implies that Epi-Fe 3+ might modulate neural activity of Epi in CNS.
PB  - Belgrade : Faculty of Chemistry : Serbian Biochemical Society
C3  - Proceedings: Serbian Biochemical Society Eigth Conference with international participation: Coordination in Biochemistry and Life; 2018 Nov 16; Novi Sad, Serbia
T1  - Redox interactions of epinephrine with iron at physiological pH
SP  - 141
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5517
ER  - 

@conference{
author = "Korać, Jelena and Stanković, Dalibor and Bogdanović Pristov, Jelena and Nikolić, Ljiljana and Spasojević, Ivan",
year = "2018",
abstract = "Epinephrine ((R)-4-(1-hydroxy-2-(methylamino)ethyl)-benzene-1,2-diol (Epi) is
catecholamine that is released by the sympathetic nervous system and adrenal medulla. It
is a physiologically important molecule that acts as a hormone, neurotransmitter, and
medication with a broad range of effects 1-3 . Coordinate and redox interaction of Epi with
iron affects the interactions with other molecules and its biological effects 4
. In this study,
we reported details of redox interactions of Epi with Fe 2+ at pH 7.4, which correspond to
the pH value of human plasma Epi and Fe 2+ form a complex that acts as a strong reducing
agent. Cyclic voltammetry showed that the positions of E pa and E pc potentials were at
approximately -480 and -1100 mV. This implies that Epi and Fe 2+ build a complex with
unique redox properties. E1/2 was significantly lower compared to E0' for O 2 /O 2•- (-350
mV). It is important to point out this because superoxide radical anion is produced via
spontaneous Fe 2+ reaction with O 2. In other words, Epi-Fe 2+ complex should be capable of
reducing transition metals in (patho)physiologicaly relevant complexes that are not
susceptible to reduction by O 2. Our results confirmed that Epi-Fe
2+ is capable of reducing
the S-S group of glutathione disulfide. On the other hand, Epi acted in a catalyst-like
fashion to promote Fe 2+ oxidation by molecular oxygen, and to a facilitated formation of
the Epi–Fe 3+ complexes, at physiological pH. In addition, we examined the effects of
epinepfrine and Epi/Fe3+ system on glioma cells. Epinephrine alone evokes changes in the
membrane currents of glioma cells, but such effects were not observed for the complex
with Fe 3+
. This implies that Epi-Fe 3+ might modulate neural activity of Epi in CNS.",
publisher = "Belgrade : Faculty of Chemistry : Serbian Biochemical Society",
journal = "Proceedings: Serbian Biochemical Society Eigth Conference with international participation: Coordination in Biochemistry and Life; 2018 Nov 16; Novi Sad, Serbia",
title = "Redox interactions of epinephrine with iron at physiological pH",
pages = "141",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5517"
}

Korać, J., Stanković, D., Bogdanović Pristov, J., Nikolić, L.,& Spasojević, I.. (2018). Redox interactions of epinephrine with iron at physiological pH. in Proceedings: Serbian Biochemical Society Eigth Conference with international participation: Coordination in Biochemistry and Life; 2018 Nov 16; Novi Sad, Serbia
Belgrade : Faculty of Chemistry : Serbian Biochemical Society., 141.
https://hdl.handle.net/21.15107/rcub_ibiss_5517

Korać J, Stanković D, Bogdanović Pristov J, Nikolić L, Spasojević I. Redox interactions of epinephrine with iron at physiological pH. in Proceedings: Serbian Biochemical Society Eigth Conference with international participation: Coordination in Biochemistry and Life; 2018 Nov 16; Novi Sad, Serbia. 2018;:141.
https://hdl.handle.net/21.15107/rcub_ibiss_5517 .

Korać, Jelena, Stanković, Dalibor, Bogdanović Pristov, Jelena, Nikolić, Ljiljana, Spasojević, Ivan, "Redox interactions of epinephrine with iron at physiological pH" in Proceedings: Serbian Biochemical Society Eigth Conference with international participation: Coordination in Biochemistry and Life; 2018 Nov 16; Novi Sad, Serbia (2018):141,
https://hdl.handle.net/21.15107/rcub_ibiss_5517 .