TY  - JOUR
AU  - Stojičić, Dragana
AU  - Tošić, Svetlana
AU  - Stojanović, Gordana
AU  - Zlatković, Bojan
AU  - Jovanović, Snežana
AU  - Budimir, Snežana
AU  - Uzelac, Branka
PY  - 2022
UR  - https://www.mdpi.com/2223-7747/11/2/198
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8778064
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4768
AB  - Clinopodium pulegium (Rochel) Bräuchler (Lamiaceae) is an endangered species endemic to the Southern Carpathians. It is characterized by the production of high amounts of essential oils, which emit volatile organic compounds (VOCs) that have an essential role in biotic and abiotic stress responses and in plant-plant and plant-insect interactions. The present study was initiated to phytochemically examine the influence of different carbon sources in the nutrition medium on VOC emissions of micropropagated C. pulegium plants, using gas chromatography-mass spectrometry analysis of headspace VOCs. The volatile profiles were subjected to multivariate analysis with respect to the presence, concentration and type of carbon source in the nutrient medium. In addition, the effect of different carbohydrates on the density and size of the leaf glandular trichomes, the main structures involved in the emission of VOCs, was determined. A total of 19 VOCs, primarily belonging to mono- and sesquiterpenes previously described in plants, were tentatively identified. Six VOCs were produced at levels higher than 2% of the total VOC emission, dominated by pulegone, ß-pinene and menthone. Inclusion of the carbohydrates in the culture media affected the production of the main leaf trichome-associated volatile allelochemicals although the qualitative composition of the volatiles changed only slightly. Multivariate analysis showed that the concentration, rather than the carbohydrate type, influenced the VOC profile.
PB  - Basel: MDPI
T2  - Plants (Basel, Switzerland)
T1  - Volatile Organic Compound Composition and Glandular Trichome Characteristics of In Vitro Propagated Clinopodium pulegium (Rochel) Bräuchler: Effect of Carbon Source.
IS  - 2
VL  - 11
DO  - 10.3390/plants11020198
SP  - 198
ER  - 

@article{
author = "Stojičić, Dragana and Tošić, Svetlana and Stojanović, Gordana and Zlatković, Bojan and Jovanović, Snežana and Budimir, Snežana and Uzelac, Branka",
year = "2022",
abstract = "Clinopodium pulegium (Rochel) Bräuchler (Lamiaceae) is an endangered species endemic to the Southern Carpathians. It is characterized by the production of high amounts of essential oils, which emit volatile organic compounds (VOCs) that have an essential role in biotic and abiotic stress responses and in plant-plant and plant-insect interactions. The present study was initiated to phytochemically examine the influence of different carbon sources in the nutrition medium on VOC emissions of micropropagated C. pulegium plants, using gas chromatography-mass spectrometry analysis of headspace VOCs. The volatile profiles were subjected to multivariate analysis with respect to the presence, concentration and type of carbon source in the nutrient medium. In addition, the effect of different carbohydrates on the density and size of the leaf glandular trichomes, the main structures involved in the emission of VOCs, was determined. A total of 19 VOCs, primarily belonging to mono- and sesquiterpenes previously described in plants, were tentatively identified. Six VOCs were produced at levels higher than 2% of the total VOC emission, dominated by pulegone, ß-pinene and menthone. Inclusion of the carbohydrates in the culture media affected the production of the main leaf trichome-associated volatile allelochemicals although the qualitative composition of the volatiles changed only slightly. Multivariate analysis showed that the concentration, rather than the carbohydrate type, influenced the VOC profile.",
publisher = "Basel: MDPI",
journal = "Plants (Basel, Switzerland)",
title = "Volatile Organic Compound Composition and Glandular Trichome Characteristics of In Vitro Propagated Clinopodium pulegium (Rochel) Bräuchler: Effect of Carbon Source.",
number = "2",
volume = "11",
doi = "10.3390/plants11020198",
pages = "198"
}

Stojičić, D., Tošić, S., Stojanović, G., Zlatković, B., Jovanović, S., Budimir, S.,& Uzelac, B.. (2022). Volatile Organic Compound Composition and Glandular Trichome Characteristics of In Vitro Propagated Clinopodium pulegium (Rochel) Bräuchler: Effect of Carbon Source.. in Plants (Basel, Switzerland)
Basel: MDPI., 11(2), 198.
https://doi.org/10.3390/plants11020198

Stojičić D, Tošić S, Stojanović G, Zlatković B, Jovanović S, Budimir S, Uzelac B. Volatile Organic Compound Composition and Glandular Trichome Characteristics of In Vitro Propagated Clinopodium pulegium (Rochel) Bräuchler: Effect of Carbon Source.. in Plants (Basel, Switzerland). 2022;11(2):198.
doi:10.3390/plants11020198 .

Stojičić, Dragana, Tošić, Svetlana, Stojanović, Gordana, Zlatković, Bojan, Jovanović, Snežana, Budimir, Snežana, Uzelac, Branka, "Volatile Organic Compound Composition and Glandular Trichome Characteristics of In Vitro Propagated Clinopodium pulegium (Rochel) Bräuchler: Effect of Carbon Source." in Plants (Basel, Switzerland), 11, no. 2 (2022):198,
https://doi.org/10.3390/plants11020198 . .

TY  - CONF
AU  - Uzelac, Branka
AU  - Stojičić, Dragana
AU  - Budimir, Snežana
AU  - Tošić, Svetlana
AU  - Zlatković, Bojan
AU  - Blagojević, Saša
AU  - Manić, Branislav
AU  - Janjanin, Mirjana
AU  - Slavkovska, Violeta
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5664
AB  - Secondary metabolism in plant plays a major role in the survival of the plant in its ecosystem, mediating the interaction of the plant with its environment. Plant bioactive compounds are biosynthesized as a defensive strategy of plants in response to natural perturbations. A number of biological effects have been associated with the main monoterpenoids detected in investigated Micromeria spp. and Clinopodium spp. essential oils. One alternative for the production of these prospective biocontrol products is in vitro plant tissue culture. Our data suggest that the metabolic potential of in vitro shoot cultures of selected species can be manipulated by varying in vitro culture conditions.
PB  - Kragujevac: Agronomsku fakultet u Čačku, Univerzitet u Kragujevcu
C3  - Zbornik radova: XXVII savetovanje o biotehnologiji sa međunarodnim učešćem; 2022 Mar 25-26; Čačak, Serbia
T1  - Essential oils as potential biocontrol products against plant pathogens and weeds: in vitro culture approach
DO  - 10.46793/SBT27.345U
SP  - 345
EP  - 350
ER  - 

@conference{
author = "Uzelac, Branka and Stojičić, Dragana and Budimir, Snežana and Tošić, Svetlana and Zlatković, Bojan and Blagojević, Saša and Manić, Branislav and Janjanin, Mirjana and Slavkovska, Violeta",
year = "2022",
abstract = "Secondary metabolism in plant plays a major role in the survival of the plant in its ecosystem, mediating the interaction of the plant with its environment. Plant bioactive compounds are biosynthesized as a defensive strategy of plants in response to natural perturbations. A number of biological effects have been associated with the main monoterpenoids detected in investigated Micromeria spp. and Clinopodium spp. essential oils. One alternative for the production of these prospective biocontrol products is in vitro plant tissue culture. Our data suggest that the metabolic potential of in vitro shoot cultures of selected species can be manipulated by varying in vitro culture conditions.",
publisher = "Kragujevac: Agronomsku fakultet u Čačku, Univerzitet u Kragujevcu",
journal = "Zbornik radova: XXVII savetovanje o biotehnologiji sa međunarodnim učešćem; 2022 Mar 25-26; Čačak, Serbia",
title = "Essential oils as potential biocontrol products against plant pathogens and weeds: in vitro culture approach",
doi = "10.46793/SBT27.345U",
pages = "345-350"
}

Uzelac, B., Stojičić, D., Budimir, S., Tošić, S., Zlatković, B., Blagojević, S., Manić, B., Janjanin, M.,& Slavkovska, V.. (2022). Essential oils as potential biocontrol products against plant pathogens and weeds: in vitro culture approach. in Zbornik radova: XXVII savetovanje o biotehnologiji sa međunarodnim učešćem; 2022 Mar 25-26; Čačak, Serbia
Kragujevac: Agronomsku fakultet u Čačku, Univerzitet u Kragujevcu., 345-350.
https://doi.org/10.46793/SBT27.345U

Uzelac B, Stojičić D, Budimir S, Tošić S, Zlatković B, Blagojević S, Manić B, Janjanin M, Slavkovska V. Essential oils as potential biocontrol products against plant pathogens and weeds: in vitro culture approach. in Zbornik radova: XXVII savetovanje o biotehnologiji sa međunarodnim učešćem; 2022 Mar 25-26; Čačak, Serbia. 2022;:345-350.
doi:10.46793/SBT27.345U .

Uzelac, Branka, Stojičić, Dragana, Budimir, Snežana, Tošić, Svetlana, Zlatković, Bojan, Blagojević, Saša, Manić, Branislav, Janjanin, Mirjana, Slavkovska, Violeta, "Essential oils as potential biocontrol products against plant pathogens and weeds: in vitro culture approach" in Zbornik radova: XXVII savetovanje o biotehnologiji sa međunarodnim učešćem; 2022 Mar 25-26; Čačak, Serbia (2022):345-350,
https://doi.org/10.46793/SBT27.345U . .

TY  - CONF
AU  - Stojičić, Dragana
AU  - Tošić, Svetlana
AU  - Zlatković, Bojan
AU  - Blagojević, Saša
AU  - Manić, Branislav
AU  - Budimir, Snežana
AU  - Uzelac, Branka
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5662
AB  - Clinopodium thymifolium (Scop.) Kuntze (syn. Micromeria thymifolia (Scop.) Fritsch) is a Balkan endemic species, traditionally used as a condiment and medicinal plant in the Mediterranean area. Clinopodium species were shown to produce considerable quantities (> 0.5%) of essential oils. The essential oil of wild-growing C. thymifolium possesses high antimicrobial activity. One alternative for the production of commercially important plant-derived metabolites, especially of rare plant genotypes, is in vitro plant tissue culture, given that the metabolic potential of in vitro shoot cultures can be manipulated by varying in vitro culture conditions. In view of the potential pharmacological and commercial value of C. thymifolium, the present study was initiated to propagate this aromatic plant species with the aim to increase the accumulation of biomass and the production of secondary metabolites. Shoots of wild-growing plants, dissected into one-node stem segments bearing two axillary buds, were used to establish in vitro cultures. Shoot multiplication was carried out on basal nutritional medium and on medium supplemented with various plant growth regulators at different concentrations. Nodal segments developed axillary shoots, and were successfully propagated on basal medium, whereas tested plant growth regulators differently affected their multiplication and biomass production.
PB  - Niš: Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
C3  - Book of abstracts: 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions; 2022 Jun 26-29; Kladovo, Serbia
T1  - Micropropagation of Clinopodium thymifolium (Scop.) Kuntze (Lamiaceae)
SP  - 113
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5662
ER  - 

@conference{
editor = "Ranđelović, Vladimir, Stojanović-Radić, Zorica, Nikolić, Danijela, Jenačković Gocić, Dragana",
author = "Stojičić, Dragana and Tošić, Svetlana and Zlatković, Bojan and Blagojević, Saša and Manić, Branislav and Budimir, Snežana and Uzelac, Branka",
year = "2022",
abstract = "Clinopodium thymifolium (Scop.) Kuntze (syn. Micromeria thymifolia (Scop.) Fritsch) is a Balkan endemic species, traditionally used as a condiment and medicinal plant in the Mediterranean area. Clinopodium species were shown to produce considerable quantities (> 0.5%) of essential oils. The essential oil of wild-growing C. thymifolium possesses high antimicrobial activity. One alternative for the production of commercially important plant-derived metabolites, especially of rare plant genotypes, is in vitro plant tissue culture, given that the metabolic potential of in vitro shoot cultures can be manipulated by varying in vitro culture conditions. In view of the potential pharmacological and commercial value of C. thymifolium, the present study was initiated to propagate this aromatic plant species with the aim to increase the accumulation of biomass and the production of secondary metabolites. Shoots of wild-growing plants, dissected into one-node stem segments bearing two axillary buds, were used to establish in vitro cultures. Shoot multiplication was carried out on basal nutritional medium and on medium supplemented with various plant growth regulators at different concentrations. Nodal segments developed axillary shoots, and were successfully propagated on basal medium, whereas tested plant growth regulators differently affected their multiplication and biomass production.",
publisher = "Niš: Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš",
journal = "Book of abstracts: 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions; 2022 Jun 26-29; Kladovo, Serbia",
title = "Micropropagation of Clinopodium thymifolium (Scop.) Kuntze (Lamiaceae)",
pages = "113",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5662"
}

Ranđelović, V., Stojanović-Radić, Z., Nikolić, D., Jenačković Gocić, D., Stojičić, D., Tošić, S., Zlatković, B., Blagojević, S., Manić, B., Budimir, S.,& Uzelac, B.. (2022). Micropropagation of Clinopodium thymifolium (Scop.) Kuntze (Lamiaceae). in Book of abstracts: 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions; 2022 Jun 26-29; Kladovo, Serbia
Niš: Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 113.
https://hdl.handle.net/21.15107/rcub_ibiss_5662

Ranđelović V, Stojanović-Radić Z, Nikolić D, Jenačković Gocić D, Stojičić D, Tošić S, Zlatković B, Blagojević S, Manić B, Budimir S, Uzelac B. Micropropagation of Clinopodium thymifolium (Scop.) Kuntze (Lamiaceae). in Book of abstracts: 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions; 2022 Jun 26-29; Kladovo, Serbia. 2022;:113.
https://hdl.handle.net/21.15107/rcub_ibiss_5662 .

Ranđelović, Vladimir, Stojanović-Radić, Zorica, Nikolić, Danijela, Jenačković Gocić, Dragana, Stojičić, Dragana, Tošić, Svetlana, Zlatković, Bojan, Blagojević, Saša, Manić, Branislav, Budimir, Snežana, Uzelac, Branka, "Micropropagation of Clinopodium thymifolium (Scop.) Kuntze (Lamiaceae)" in Book of abstracts: 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions; 2022 Jun 26-29; Kladovo, Serbia (2022):113,
https://hdl.handle.net/21.15107/rcub_ibiss_5662 .

TY  - CONF
AU  - Janjanin, Mirjana
AU  - Tošić, Svetlana
AU  - Stojičić, Dragana
AU  - Zlatković, Bojan
AU  - Budimir, Snežana
AU  - Uzelac, Branka
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6719
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Proceedings: 14th Multinational Congress on Microscopy; 2019 Sep 15-20; Belgrade, Serbia
T1  - Micromorphological traits of Micromeria graeca (L.) Benth. ex Rchb. (Lamiaceae) leaf glandular trichomes of in vitro propagated plants
DO  - 978-86-80335-11-7
SP  - 154
EP  - 156
ER  - 

@conference{
author = "Janjanin, Mirjana and Tošić, Svetlana and Stojičić, Dragana and Zlatković, Bojan and Budimir, Snežana and Uzelac, Branka",
year = "2019",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Proceedings: 14th Multinational Congress on Microscopy; 2019 Sep 15-20; Belgrade, Serbia",
title = "Micromorphological traits of Micromeria graeca (L.) Benth. ex Rchb. (Lamiaceae) leaf glandular trichomes of in vitro propagated plants",
doi = "978-86-80335-11-7",
pages = "154-156"
}

Janjanin, M., Tošić, S., Stojičić, D., Zlatković, B., Budimir, S.,& Uzelac, B.. (2019). Micromorphological traits of Micromeria graeca (L.) Benth. ex Rchb. (Lamiaceae) leaf glandular trichomes of in vitro propagated plants. in Proceedings: 14th Multinational Congress on Microscopy; 2019 Sep 15-20; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 154-156.
https://doi.org/978-86-80335-11-7

Janjanin M, Tošić S, Stojičić D, Zlatković B, Budimir S, Uzelac B. Micromorphological traits of Micromeria graeca (L.) Benth. ex Rchb. (Lamiaceae) leaf glandular trichomes of in vitro propagated plants. in Proceedings: 14th Multinational Congress on Microscopy; 2019 Sep 15-20; Belgrade, Serbia. 2019;:154-156.
doi:978-86-80335-11-7 .

Janjanin, Mirjana, Tošić, Svetlana, Stojičić, Dragana, Zlatković, Bojan, Budimir, Snežana, Uzelac, Branka, "Micromorphological traits of Micromeria graeca (L.) Benth. ex Rchb. (Lamiaceae) leaf glandular trichomes of in vitro propagated plants" in Proceedings: 14th Multinational Congress on Microscopy; 2019 Sep 15-20; Belgrade, Serbia (2019):154-156,
https://doi.org/978-86-80335-11-7 . .

TY  - JOUR
AU  - Tošić, Svetlana
AU  - Stojičić, Dragana
AU  - Slavkovska, Violeta
AU  - Mihailov-Krstev, Tatjana
AU  - Zlatković, Bojan
AU  - Budimir, Snežana
AU  - Uzelac, Branka
PY  - 2019
UR  - http://link.springer.com/10.1007/s00425-018-03071-5
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3256
AB  - MAIN CONCLUSION In vitro culture conditions and kinetin induced quantitative modifications in the production of the major volatile constituents in Micromeria croatica plantlets. Antimicrobial activity of methanolic extracts obtained from micropropagated and wild-growing plants was evaluated. Micromeria spp. are aromatic plants, many of which were shown to exhibit various biological effects. The present study aimed to determine the content and the composition of the essential oil of in vitro-cultured Micromeria croatica (Pers.) Schott and to evaluate the in vitro antimicrobial activity of its methanolic extract, in order to compare its phytochemical profile and biological activity with wild-growing plants. Shoots regenerated on MS medium without plant growth regulators (PGRs) or supplemented with kinetin were used for phytochemical analysis. Essential oils from both native plant material and in vitro-cultivated M. croatica plants, with a total of 44 identified constituents, were dominated by oxygenated monoterpenes. Borneol was the main component detected in wild-growing plants (25.28%) and micropropagated plants grown on PGR-free medium (20.30%). Kinetin treatment led to increased oil yield and favored the production of oxygenated monoterpenes, dominated by geranial (33.53%) and cis-p-mentha-1(7),8-dien-ol (23.69%). The percentage of total sesquiterpenoids in micropropagated plant material was considerably lower than in wild-growing plants. In vitro culture conditions and PGRs affected the production of essential oils, inducing quantitative modifications in the production of the major volatile constituents in M. croatica plantlets. The antimicrobial activity of M. croatica methanolic extracts was investigated using the broth microdilution method. Extracts obtained from in vitro cultures generally exhibited greater antibacterial potential, compared to wild-growing plants. Among six bacterial strains tested, Bacillus cereus and Staphylococcus aureus were the most sensitive microorganisms. The present study provided evidence that in vitro culture conditions might favorably affect the antimicrobial activity of M. croatica methanolic extracts.
PB  - Springer Berlin Heidelberg
T2  - Planta
T1  - Phytochemical composition and biological activities of native and in vitro-propagated Micromeria croatica (Pers.) Schott (Lamiaceae).
DO  - 10.1007/s00425-018-03071-5
ER  - 

@article{
author = "Tošić, Svetlana and Stojičić, Dragana and Slavkovska, Violeta and Mihailov-Krstev, Tatjana and Zlatković, Bojan and Budimir, Snežana and Uzelac, Branka",
year = "2019",
abstract = "MAIN CONCLUSION In vitro culture conditions and kinetin induced quantitative modifications in the production of the major volatile constituents in Micromeria croatica plantlets. Antimicrobial activity of methanolic extracts obtained from micropropagated and wild-growing plants was evaluated. Micromeria spp. are aromatic plants, many of which were shown to exhibit various biological effects. The present study aimed to determine the content and the composition of the essential oil of in vitro-cultured Micromeria croatica (Pers.) Schott and to evaluate the in vitro antimicrobial activity of its methanolic extract, in order to compare its phytochemical profile and biological activity with wild-growing plants. Shoots regenerated on MS medium without plant growth regulators (PGRs) or supplemented with kinetin were used for phytochemical analysis. Essential oils from both native plant material and in vitro-cultivated M. croatica plants, with a total of 44 identified constituents, were dominated by oxygenated monoterpenes. Borneol was the main component detected in wild-growing plants (25.28%) and micropropagated plants grown on PGR-free medium (20.30%). Kinetin treatment led to increased oil yield and favored the production of oxygenated monoterpenes, dominated by geranial (33.53%) and cis-p-mentha-1(7),8-dien-ol (23.69%). The percentage of total sesquiterpenoids in micropropagated plant material was considerably lower than in wild-growing plants. In vitro culture conditions and PGRs affected the production of essential oils, inducing quantitative modifications in the production of the major volatile constituents in M. croatica plantlets. The antimicrobial activity of M. croatica methanolic extracts was investigated using the broth microdilution method. Extracts obtained from in vitro cultures generally exhibited greater antibacterial potential, compared to wild-growing plants. Among six bacterial strains tested, Bacillus cereus and Staphylococcus aureus were the most sensitive microorganisms. The present study provided evidence that in vitro culture conditions might favorably affect the antimicrobial activity of M. croatica methanolic extracts.",
publisher = "Springer Berlin Heidelberg",
journal = "Planta",
title = "Phytochemical composition and biological activities of native and in vitro-propagated Micromeria croatica (Pers.) Schott (Lamiaceae).",
doi = "10.1007/s00425-018-03071-5"
}

Tošić, S., Stojičić, D., Slavkovska, V., Mihailov-Krstev, T., Zlatković, B., Budimir, S.,& Uzelac, B.. (2019). Phytochemical composition and biological activities of native and in vitro-propagated Micromeria croatica (Pers.) Schott (Lamiaceae).. in Planta
Springer Berlin Heidelberg..
https://doi.org/10.1007/s00425-018-03071-5

Tošić S, Stojičić D, Slavkovska V, Mihailov-Krstev T, Zlatković B, Budimir S, Uzelac B. Phytochemical composition and biological activities of native and in vitro-propagated Micromeria croatica (Pers.) Schott (Lamiaceae).. in Planta. 2019;.
doi:10.1007/s00425-018-03071-5 .

Tošić, Svetlana, Stojičić, Dragana, Slavkovska, Violeta, Mihailov-Krstev, Tatjana, Zlatković, Bojan, Budimir, Snežana, Uzelac, Branka, "Phytochemical composition and biological activities of native and in vitro-propagated Micromeria croatica (Pers.) Schott (Lamiaceae)." in Planta (2019),
https://doi.org/10.1007/s00425-018-03071-5 . .

TY  - CONF
AU  - Tošić, Svetlana
AU  - Stojičić, Dragana
AU  - Slavkovska, Violeta
AU  - Zlatković, Bojan
AU  - Budimir, Snežana
AU  - Uzelac, Branka
PY  - 2017
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6735
AB  - Morphology and structure of Micromeria croatica glandular trichomes in relation to the secretory process were characterized using scanning electron microscopy (SEM) and light microscopy, and their secretion was histochemically analyzed. SEM and histochemical analysis revealed that leaf indumentum of in vitro grown M. croatica consisted of non-glandular and glandular trichomes (Figure 1a). The main types of glandular trichomes observed in M. croatica leaves were peltate and capitate (Figure 1b-d). Peltate trichomes consisted of a broad basal cell embedded in the epidermis, a single-celled stalk, and a large round multicellular head (Figure 1e, h). Two types of capitate trichomes, differing in size and structure, could be distinguished. Type I capitate trichomes were positioned at an angle to the leaf surface and composed of one basal epidermal cell, short cutinized stalk and unicellular ellipsoidal head (Figure 1f). Type II capitate trichomes comprised of one basal cell, unicellular stalk and one apical secretory cell (Figure 1g). Apical cell of mature type II capitate trichomes had well developed round subcuticular space (Figure 1d). Plant extracts secreted from glandular trichomes are a valuable source of biologically active compounds. Phytochemical analysis identified up to 37 compounds in the essential oils (EOs) of wild-growing and micropropagated plants, and revealed differences in both content and the composition of the EOs obtained from different samples. EOs from both native plant material and in vitro cultivated plants showed very high percentages of total monoterpenoids, dominated by oxygenated monoterpenes. 
Borneol was the main essential oil component detected in wild-growing plants (25.28%) and micropropagated plants grown on plant growth regulator-free medium (20.30%). Micropropagated plants cultivated in the presence of 0.3 µM kinetin had the highest yield of the EO, with geranial (33.53%) and cis-p-mentha-1(7),8-dien-ol (23.69%) being the main EO components. The percentage of total sesquiterpenoids in micropropagated plant material was considerably lower than in wild-growing plants. Sesquiterpene hydrocarbons accounted for the majority of detected sesquiterpenoids and were dominated by alpha-cadinene and beta-vetivenene. Some of the volatiles were found only in plant material obtained by micropropagation but not in wild-growing plants. Differences in both content and the composition of the essential oils obtained from different samples indicated that in vitro culture conditions and plant growth regulators significantly influence the essential oils properties.
PB  - Zagreb: Ruđer Bošković Institute and Croatian Microscopy Society
C3  - Book of Abstract: 13th Multinational Congress on Microscopy; 2017 Sep 24-29; Rovinj, Croatia
T1  - Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria croatica (Pers.) Schott (Lamiaceae)
SP  - 302
EP  - 303
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6735
ER  - 

@conference{
author = "Tošić, Svetlana and Stojičić, Dragana and Slavkovska, Violeta and Zlatković, Bojan and Budimir, Snežana and Uzelac, Branka",
year = "2017",
abstract = "Morphology and structure of Micromeria croatica glandular trichomes in relation to the secretory process were characterized using scanning electron microscopy (SEM) and light microscopy, and their secretion was histochemically analyzed. SEM and histochemical analysis revealed that leaf indumentum of in vitro grown M. croatica consisted of non-glandular and glandular trichomes (Figure 1a). The main types of glandular trichomes observed in M. croatica leaves were peltate and capitate (Figure 1b-d). Peltate trichomes consisted of a broad basal cell embedded in the epidermis, a single-celled stalk, and a large round multicellular head (Figure 1e, h). Two types of capitate trichomes, differing in size and structure, could be distinguished. Type I capitate trichomes were positioned at an angle to the leaf surface and composed of one basal epidermal cell, short cutinized stalk and unicellular ellipsoidal head (Figure 1f). Type II capitate trichomes comprised of one basal cell, unicellular stalk and one apical secretory cell (Figure 1g). Apical cell of mature type II capitate trichomes had well developed round subcuticular space (Figure 1d). Plant extracts secreted from glandular trichomes are a valuable source of biologically active compounds. Phytochemical analysis identified up to 37 compounds in the essential oils (EOs) of wild-growing and micropropagated plants, and revealed differences in both content and the composition of the EOs obtained from different samples. EOs from both native plant material and in vitro cultivated plants showed very high percentages of total monoterpenoids, dominated by oxygenated monoterpenes. 
Borneol was the main essential oil component detected in wild-growing plants (25.28%) and micropropagated plants grown on plant growth regulator-free medium (20.30%). Micropropagated plants cultivated in the presence of 0.3 µM kinetin had the highest yield of the EO, with geranial (33.53%) and cis-p-mentha-1(7),8-dien-ol (23.69%) being the main EO components. The percentage of total sesquiterpenoids in micropropagated plant material was considerably lower than in wild-growing plants. Sesquiterpene hydrocarbons accounted for the majority of detected sesquiterpenoids and were dominated by alpha-cadinene and beta-vetivenene. Some of the volatiles were found only in plant material obtained by micropropagation but not in wild-growing plants. Differences in both content and the composition of the essential oils obtained from different samples indicated that in vitro culture conditions and plant growth regulators significantly influence the essential oils properties.",
publisher = "Zagreb: Ruđer Bošković Institute and Croatian Microscopy Society",
journal = "Book of Abstract: 13th Multinational Congress on Microscopy; 2017 Sep 24-29; Rovinj, Croatia",
title = "Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria croatica (Pers.) Schott (Lamiaceae)",
pages = "302-303",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6735"
}

Tošić, S., Stojičić, D., Slavkovska, V., Zlatković, B., Budimir, S.,& Uzelac, B.. (2017). Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria croatica (Pers.) Schott (Lamiaceae). in Book of Abstract: 13th Multinational Congress on Microscopy; 2017 Sep 24-29; Rovinj, Croatia
Zagreb: Ruđer Bošković Institute and Croatian Microscopy Society., 302-303.
https://hdl.handle.net/21.15107/rcub_ibiss_6735

Tošić S, Stojičić D, Slavkovska V, Zlatković B, Budimir S, Uzelac B. Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria croatica (Pers.) Schott (Lamiaceae). in Book of Abstract: 13th Multinational Congress on Microscopy; 2017 Sep 24-29; Rovinj, Croatia. 2017;:302-303.
https://hdl.handle.net/21.15107/rcub_ibiss_6735 .

Tošić, Svetlana, Stojičić, Dragana, Slavkovska, Violeta, Zlatković, Bojan, Budimir, Snežana, Uzelac, Branka, "Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria croatica (Pers.) Schott (Lamiaceae)" in Book of Abstract: 13th Multinational Congress on Microscopy; 2017 Sep 24-29; Rovinj, Croatia (2017):302-303,
https://hdl.handle.net/21.15107/rcub_ibiss_6735 .

TY  - JOUR
AU  - Stojičić, Dragana
AU  - Tošić, Svetlana
AU  - Slavkovska, Violeta
AU  - Zlatković, Bojan
AU  - Budimir, Snežana
AU  - Janošević, Dušica
AU  - Uzelac, Branka
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6200
AB  - The content and chemical composition of Micromeria
pulegium (Rochel) Benth. essential oils were studied in
native plant material at vegetative stage and in micropropagated
plants, obtained from nodal segments cultured
on solid MS medium supplemented with N6–benzyladenine
(BA) or kinetin at different concentrations, alone or in
combination with indole-3-acetic acid. Shoot proliferation
was achieved in all treatments, but the highest biomass
production was obtained after treatment with 10 μM BA.
Phytochemical analysis identified up to 21 compounds in
the essential oils of wild-growing and in vitro cultivated
plants, both showing very high percentages of total
monoterpenoids dominated by oxygenated monoterpenes
of the menthane type. Pulegone and menthone were the
main essential oil components detected in both wildgrowing
plants (60.07 and 26.85 %, respectively) and
micropropagated plants grown on either plant growth regulator-
free medium (44.57 and 29.14 %, respectively) or
BA-supplemented medium (50.77 and 14.45 %, respectively).
The percentage of total sesquiterpenoids increased
in vitro, particularly owing to sesquiterpene hydrocarbons
that were not found in wild-growing plants. Differences in
both content and the composition of the essential oils
obtained from different samples indicated that in vitro
culture conditions and plant growth regulators significantly
influence the essential oils properties. In addition, the
morphology and structure of M. pulegium glandular trichomes
in relation to the secretory process were characterized
for the first time using SEM and light microscopy,
and their secretion was histochemically analyzed.
PB  - Berlin Heidelberg : Springer-Verlag
T2  - Planta
T1  - Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria pulegium (Rochel) Benth. (Lamiaceae)
IS  - 2
VL  - 244
DO  - 10.1007/s00425-016-2513-7
SP  - 393
EP  - 404
ER  - 

@article{
author = "Stojičić, Dragana and Tošić, Svetlana and Slavkovska, Violeta and Zlatković, Bojan and Budimir, Snežana and Janošević, Dušica and Uzelac, Branka",
year = "2016",
abstract = "The content and chemical composition of Micromeria
pulegium (Rochel) Benth. essential oils were studied in
native plant material at vegetative stage and in micropropagated
plants, obtained from nodal segments cultured
on solid MS medium supplemented with N6–benzyladenine
(BA) or kinetin at different concentrations, alone or in
combination with indole-3-acetic acid. Shoot proliferation
was achieved in all treatments, but the highest biomass
production was obtained after treatment with 10 μM BA.
Phytochemical analysis identified up to 21 compounds in
the essential oils of wild-growing and in vitro cultivated
plants, both showing very high percentages of total
monoterpenoids dominated by oxygenated monoterpenes
of the menthane type. Pulegone and menthone were the
main essential oil components detected in both wildgrowing
plants (60.07 and 26.85 %, respectively) and
micropropagated plants grown on either plant growth regulator-
free medium (44.57 and 29.14 %, respectively) or
BA-supplemented medium (50.77 and 14.45 %, respectively).
The percentage of total sesquiterpenoids increased
in vitro, particularly owing to sesquiterpene hydrocarbons
that were not found in wild-growing plants. Differences in
both content and the composition of the essential oils
obtained from different samples indicated that in vitro
culture conditions and plant growth regulators significantly
influence the essential oils properties. In addition, the
morphology and structure of M. pulegium glandular trichomes
in relation to the secretory process were characterized
for the first time using SEM and light microscopy,
and their secretion was histochemically analyzed.",
publisher = "Berlin Heidelberg : Springer-Verlag",
journal = "Planta",
title = "Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria pulegium (Rochel) Benth. (Lamiaceae)",
number = "2",
volume = "244",
doi = "10.1007/s00425-016-2513-7",
pages = "393-404"
}

Stojičić, D., Tošić, S., Slavkovska, V., Zlatković, B., Budimir, S., Janošević, D.,& Uzelac, B.. (2016). Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria pulegium (Rochel) Benth. (Lamiaceae). in Planta
Berlin Heidelberg : Springer-Verlag., 244(2), 393-404.
https://doi.org/10.1007/s00425-016-2513-7

Stojičić D, Tošić S, Slavkovska V, Zlatković B, Budimir S, Janošević D, Uzelac B. Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria pulegium (Rochel) Benth. (Lamiaceae). in Planta. 2016;244(2):393-404.
doi:10.1007/s00425-016-2513-7 .

Stojičić, Dragana, Tošić, Svetlana, Slavkovska, Violeta, Zlatković, Bojan, Budimir, Snežana, Janošević, Dušica, Uzelac, Branka, "Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria pulegium (Rochel) Benth. (Lamiaceae)" in Planta, 244, no. 2 (2016):393-404,
https://doi.org/10.1007/s00425-016-2513-7 . .