TY  - JOUR
AU  - Lavrnja, Irena
AU  - Parabucki, Ana
AU  - Brkić, Predrag
AU  - Jovanović, Tomislav
AU  - Dacic, Sanja
AU  - Savić, Danijela
AU  - Pantić, Igor
AU  - Stojiljković, Mirjana
AU  - Peković, Sanja
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2070
AB  - The exact mechanisms by which treatment with hyperbaric oxygen (HBOT)
   exerts its beneficial effects on recovery after brain injury are still
   unrevealed. Therefore, in this study we investigated the influence of
   repetitive HBOT on the reactive astrogliosis and expression of mediators
   of inflammation after cortical stab injury (CSI). CSI was performed on
   male Wistar rats, divided into control, sham, and lesioned groups with
   appropriate HBO. The HBOT protocol was as follows: 10 minutes of slow
   compression, 2.5 atmospheres absolute (ATA) for 60 minutes, and 10
   minutes of slow decompression, once a day for 10 consecutive days. Data
   obtained using real-time polymerase chain reaction, Western blot, and
   immunohistochemical and immunofluorescence analyses revealed that
   repetitive HBOT applied after the CSI attenuates reactive astrogliosis
   and glial scarring, and reduces expression of GFAP (glial fibrillary
   acidic protein), vimentin, and ICAM-1 (intercellular adhesion
   molecule-1) both at gene and tissue levels. In addition, HBOT prevents
   expression of CD40 and its ligand CD40L on microglia, neutrophils,
   cortical neurons, and reactive astrocytes. Accordingly, repetitive HBOT,
   by prevention of glial scarring and limiting of expression of
   inflammatory mediators, supports formation of more permissive
   environment for repair and regeneration.
T2  - Mediators of Inflammation
T1  - Repetitive Hyperbaric Oxygenation Attenuates Reactive Astrogliosis and
 Suppresses Expression of Inflammatory Mediators in the Rat Model of
 Brain Injury
IS  - 498405
DO  - 10.1155/2015/498405
ER  - 

@article{
author = "Lavrnja, Irena and Parabucki, Ana and Brkić, Predrag and Jovanović, Tomislav and Dacic, Sanja and Savić, Danijela and Pantić, Igor and Stojiljković, Mirjana and Peković, Sanja",
year = "2015",
abstract = "The exact mechanisms by which treatment with hyperbaric oxygen (HBOT)
   exerts its beneficial effects on recovery after brain injury are still
   unrevealed. Therefore, in this study we investigated the influence of
   repetitive HBOT on the reactive astrogliosis and expression of mediators
   of inflammation after cortical stab injury (CSI). CSI was performed on
   male Wistar rats, divided into control, sham, and lesioned groups with
   appropriate HBO. The HBOT protocol was as follows: 10 minutes of slow
   compression, 2.5 atmospheres absolute (ATA) for 60 minutes, and 10
   minutes of slow decompression, once a day for 10 consecutive days. Data
   obtained using real-time polymerase chain reaction, Western blot, and
   immunohistochemical and immunofluorescence analyses revealed that
   repetitive HBOT applied after the CSI attenuates reactive astrogliosis
   and glial scarring, and reduces expression of GFAP (glial fibrillary
   acidic protein), vimentin, and ICAM-1 (intercellular adhesion
   molecule-1) both at gene and tissue levels. In addition, HBOT prevents
   expression of CD40 and its ligand CD40L on microglia, neutrophils,
   cortical neurons, and reactive astrocytes. Accordingly, repetitive HBOT,
   by prevention of glial scarring and limiting of expression of
   inflammatory mediators, supports formation of more permissive
   environment for repair and regeneration.",
journal = "Mediators of Inflammation",
title = "Repetitive Hyperbaric Oxygenation Attenuates Reactive Astrogliosis and
 Suppresses Expression of Inflammatory Mediators in the Rat Model of
 Brain Injury",
number = "498405",
doi = "10.1155/2015/498405"
}

Lavrnja, I., Parabucki, A., Brkić, P., Jovanović, T., Dacic, S., Savić, D., Pantić, I., Stojiljković, M.,& Peković, S.. (2015). Repetitive Hyperbaric Oxygenation Attenuates Reactive Astrogliosis and
 Suppresses Expression of Inflammatory Mediators in the Rat Model of
 Brain Injury. in Mediators of Inflammation(498405).
https://doi.org/10.1155/2015/498405

Lavrnja I, Parabucki A, Brkić P, Jovanović T, Dacic S, Savić D, Pantić I, Stojiljković M, Peković S. Repetitive Hyperbaric Oxygenation Attenuates Reactive Astrogliosis and
 Suppresses Expression of Inflammatory Mediators in the Rat Model of
 Brain Injury. in Mediators of Inflammation. 2015;(498405).
doi:10.1155/2015/498405 .

Lavrnja, Irena, Parabucki, Ana, Brkić, Predrag, Jovanović, Tomislav, Dacic, Sanja, Savić, Danijela, Pantić, Igor, Stojiljković, Mirjana, Peković, Sanja, "Repetitive Hyperbaric Oxygenation Attenuates Reactive Astrogliosis and
 Suppresses Expression of Inflammatory Mediators in the Rat Model of
 Brain Injury" in Mediators of Inflammation, no. 498405 (2015),
https://doi.org/10.1155/2015/498405 . .

TY  - JOUR
AU  - Pantic, Igor
AU  - Dacic, Sanja
AU  - Brkic, Predrag
AU  - Lavrnja, Irena
AU  - Jovanovic, Tomislav
AU  - Pantic, Senka
AU  - Peković, Sanja
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1972
AB  - Fractal and grey level co-occurrence matrix (GLCM) analysis represent
   two mathematical computer-assisted algorithms that are today thought to
   be able to accurately detect and quantify changes in tissue architecture
   during various physiological and pathological processes. However,
   despite their numerous applications in histology and pathology, their
   sensitivity, specificity and validity regarding evaluation of brain
   tissue remain unclear. In this article we present the results indicating
   that certain parameters of fractal and GLCM analysis have high
   discriminatory ability in distinguishing two morphologically similar
   regions of rat hippocampus: stratum lacunosum-moleculare and stratum
   radiatum. Fractal and GLCM algorithms were performed on a total of 240
   thionine-stained hippocampus micrographs of 12 male Wistar albino rats.
   120 digital micrographs represented stratum lacunosum-moleculare, and
   another 120 stratum radiatum. For each image, 7 parameters were
   calculated: fractal dimension, lacunarity, GLCM angular second moment,
   GLCM contrast, inverse difference moment, GLCM correlation, and GLCM
   variance. GLCM variance (VAR) resulted in the largest area under the
   Receiver operating characteristic (ROC) curve of 0.96, demonstrating an
   outstanding discriminatory power in analysis of stratum
   lacunosum-moleculare (average VAR equaled 478.1 +/- 179.8) and stratum
   radiatum (average VAR of 145.9 +/- 59.2, p <0.0001). For the criterion
   VAR <= 227.5, sensitivity and specificity were 90\% and 86.7\%,
   respectively. GLCM correlation as a parameter also produced large area
   under the ROC curve of 0.95. Our results are in accordance with the
   findings of our previous study regarding brain white mass fractal and
   textural analysis. GLCM algorithm as an image analysis method has
   potentially high applicability in structural analysis of brain tissue
   cytoarcitecture. (C) 2015 Elsevier Ltd. All rights reserved.
T2  - Journal of Theoretical Biology
T1  - Discriminatory ability of fractal and grey level co-occurrence matrix
 methods in structural analysis of hippocampus layers
VL  - 370
DO  - 10.1016/j.jtbi.2015.01.035
SP  - 151
EP  - 156
ER  - 

@article{
author = "Pantic, Igor and Dacic, Sanja and Brkic, Predrag and Lavrnja, Irena and Jovanovic, Tomislav and Pantic, Senka and Peković, Sanja",
year = "2015",
abstract = "Fractal and grey level co-occurrence matrix (GLCM) analysis represent
   two mathematical computer-assisted algorithms that are today thought to
   be able to accurately detect and quantify changes in tissue architecture
   during various physiological and pathological processes. However,
   despite their numerous applications in histology and pathology, their
   sensitivity, specificity and validity regarding evaluation of brain
   tissue remain unclear. In this article we present the results indicating
   that certain parameters of fractal and GLCM analysis have high
   discriminatory ability in distinguishing two morphologically similar
   regions of rat hippocampus: stratum lacunosum-moleculare and stratum
   radiatum. Fractal and GLCM algorithms were performed on a total of 240
   thionine-stained hippocampus micrographs of 12 male Wistar albino rats.
   120 digital micrographs represented stratum lacunosum-moleculare, and
   another 120 stratum radiatum. For each image, 7 parameters were
   calculated: fractal dimension, lacunarity, GLCM angular second moment,
   GLCM contrast, inverse difference moment, GLCM correlation, and GLCM
   variance. GLCM variance (VAR) resulted in the largest area under the
   Receiver operating characteristic (ROC) curve of 0.96, demonstrating an
   outstanding discriminatory power in analysis of stratum
   lacunosum-moleculare (average VAR equaled 478.1 +/- 179.8) and stratum
   radiatum (average VAR of 145.9 +/- 59.2, p <0.0001). For the criterion
   VAR <= 227.5, sensitivity and specificity were 90\% and 86.7\%,
   respectively. GLCM correlation as a parameter also produced large area
   under the ROC curve of 0.95. Our results are in accordance with the
   findings of our previous study regarding brain white mass fractal and
   textural analysis. GLCM algorithm as an image analysis method has
   potentially high applicability in structural analysis of brain tissue
   cytoarcitecture. (C) 2015 Elsevier Ltd. All rights reserved.",
journal = "Journal of Theoretical Biology",
title = "Discriminatory ability of fractal and grey level co-occurrence matrix
 methods in structural analysis of hippocampus layers",
volume = "370",
doi = "10.1016/j.jtbi.2015.01.035",
pages = "151-156"
}

Pantic, I., Dacic, S., Brkic, P., Lavrnja, I., Jovanovic, T., Pantic, S.,& Peković, S.. (2015). Discriminatory ability of fractal and grey level co-occurrence matrix
 methods in structural analysis of hippocampus layers. in Journal of Theoretical Biology, 370, 151-156.
https://doi.org/10.1016/j.jtbi.2015.01.035

Pantic I, Dacic S, Brkic P, Lavrnja I, Jovanovic T, Pantic S, Peković S. Discriminatory ability of fractal and grey level co-occurrence matrix
 methods in structural analysis of hippocampus layers. in Journal of Theoretical Biology. 2015;370:151-156.
doi:10.1016/j.jtbi.2015.01.035 .

Pantic, Igor, Dacic, Sanja, Brkic, Predrag, Lavrnja, Irena, Jovanovic, Tomislav, Pantic, Senka, Peković, Sanja, "Discriminatory ability of fractal and grey level co-occurrence matrix
 methods in structural analysis of hippocampus layers" in Journal of Theoretical Biology, 370 (2015):151-156,
https://doi.org/10.1016/j.jtbi.2015.01.035 . .

TY  - JOUR
AU  - Brkic, Predrag
AU  - Sanja, Pekovic
AU  - Krstic, Danijela
AU  - Jovanovic, Tomislav
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2234
AB  - In recent years significant amount of data have been published in the
   filed of hyperbaric oxygenation (HBO) and traumatic brain injury (TBI).
   The main rational for the research in this field is that in TBI patients
   with the existence of dormant neural tissues that maintain cellular
   homeostasis but are unable to participate in neurotransmission, the
   addition of HBO provides a favourable environment by which neuronal
   reactivation can be achieved. As hyperbaric oxygen therapy is not
   all-or-nothing phenomena and the consequences of TBI can vary from mild
   to moderate and severe, it is important to evaluate each TBI patient
   before referring him/her to hyperbaric oxygen therapy (HBOT). Reports
   from the clinical trial that were investigating the effects of HBO on
   severe TBI show promising results. For example, significant reduction in
   mortality rates and improvement in favourable neurological outcomes were
   reported. However, conflicting results have been reported from trials
   that investigated the effects of HBO on mild and moderate TBI. The
   results from the experimental studies indicate that HBO can preserve
   mitochondrial function, reduce apoptosis and neuroinflammation and
   promote neuronal plasticity. Therefore, conducting of
   methodologically-based multicentric clinical trials is necessary to
   determine proper guidelines for inclusion of TBI patients in HBOT. As
   many reports have stated that even a few exposures to HBO can contribute
   to the recovery process, future research must be aimed at establishing
   most effective HBO protocol for TBI patients.
T2  - Periodicum Biologorum
T1  - Hyperbaric oxygenation as an adjuvant therapy for traumatic brain
 injury: a review of literature
IS  - 1
VL  - 116
SP  - 29
EP  - 36
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_2234
ER  - 

@article{
author = "Brkic, Predrag and Sanja, Pekovic and Krstic, Danijela and Jovanovic, Tomislav",
year = "2014",
abstract = "In recent years significant amount of data have been published in the
   filed of hyperbaric oxygenation (HBO) and traumatic brain injury (TBI).
   The main rational for the research in this field is that in TBI patients
   with the existence of dormant neural tissues that maintain cellular
   homeostasis but are unable to participate in neurotransmission, the
   addition of HBO provides a favourable environment by which neuronal
   reactivation can be achieved. As hyperbaric oxygen therapy is not
   all-or-nothing phenomena and the consequences of TBI can vary from mild
   to moderate and severe, it is important to evaluate each TBI patient
   before referring him/her to hyperbaric oxygen therapy (HBOT). Reports
   from the clinical trial that were investigating the effects of HBO on
   severe TBI show promising results. For example, significant reduction in
   mortality rates and improvement in favourable neurological outcomes were
   reported. However, conflicting results have been reported from trials
   that investigated the effects of HBO on mild and moderate TBI. The
   results from the experimental studies indicate that HBO can preserve
   mitochondrial function, reduce apoptosis and neuroinflammation and
   promote neuronal plasticity. Therefore, conducting of
   methodologically-based multicentric clinical trials is necessary to
   determine proper guidelines for inclusion of TBI patients in HBOT. As
   many reports have stated that even a few exposures to HBO can contribute
   to the recovery process, future research must be aimed at establishing
   most effective HBO protocol for TBI patients.",
journal = "Periodicum Biologorum",
title = "Hyperbaric oxygenation as an adjuvant therapy for traumatic brain
 injury: a review of literature",
number = "1",
volume = "116",
pages = "29-36",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_2234"
}

Brkic, P., Sanja, P., Krstic, D.,& Jovanovic, T.. (2014). Hyperbaric oxygenation as an adjuvant therapy for traumatic brain
 injury: a review of literature. in Periodicum Biologorum, 116(1), 29-36.
https://hdl.handle.net/21.15107/rcub_ibiss_2234

Brkic P, Sanja P, Krstic D, Jovanovic T. Hyperbaric oxygenation as an adjuvant therapy for traumatic brain
 injury: a review of literature. in Periodicum Biologorum. 2014;116(1):29-36.
https://hdl.handle.net/21.15107/rcub_ibiss_2234 .

Brkic, Predrag, Sanja, Pekovic, Krstic, Danijela, Jovanovic, Tomislav, "Hyperbaric oxygenation as an adjuvant therapy for traumatic brain
 injury: a review of literature" in Periodicum Biologorum, 116, no. 1 (2014):29-36,
https://hdl.handle.net/21.15107/rcub_ibiss_2234 .

TY  - JOUR
AU  - Pantic, Igor
AU  - Dacic, Sanja
AU  - Brkic, Predrag
AU  - Lavrnja, Irena
AU  - Pantic, Senka
AU  - Jovanovic, Tomislav
AU  - Peković, Sanja
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2144
AB  - This aim of this study was to assess the discriminatory value of fractal
   and grey level co-occurrence matrix (GLCM) analysis methods in standard
   microscopy analysis of two histologically similar brain white mass
   regions that have different nerve fiber orientation. A total of 160
   digital micrographs of thionine-stained rat brain white mass were
   acquired using a Pro-MicroScan DEM-200 instrument. Eighty micrographs
   from the anterior corpus callosum and eighty from the anterior cingulum
   areas of the brain were analyzed. The micrographs were evaluated using
   the National Institutes of Health ImageJ software and its plugins. For
   each micrograph, seven parameters were calculated: angular second
   moment, inverse difference moment, GLCM contrast, GLCM correlation, GLCM
   variance, fractal dimension, and lacunarity. Using the Receiver
   operating characteristic analysis, the highest discriminatory value was
   determined for inverse difference moment (IDM) (area under the receiver
   operating characteristic (ROC) curve equaled 0.925, and for the
   criterion IDM <= 0.610 the sensitivity and specificity were 82.5 and
   87.5\%, respectively). Most of the other parameters also showed good
   sensitivity and specificity. The results indicate that GLCM and fractal
   analysis methods, when applied together in brain histology analysis, are
   highly capable of discriminating white mass structures that have
   different axonal orientation.
T2  - Microscopy and Microanalysis
T1  - Application of Fractal and Grey Level Co-Occurrence Matrix Analysis in
 Evaluation of Brain Corpus Callosum and Cingulum Architecture
IS  - 5
VL  - 20
DO  - 10.1017/S1431927614012811
SP  - 1373
EP  - 1381
ER  - 

@article{
author = "Pantic, Igor and Dacic, Sanja and Brkic, Predrag and Lavrnja, Irena and Pantic, Senka and Jovanovic, Tomislav and Peković, Sanja",
year = "2014",
abstract = "This aim of this study was to assess the discriminatory value of fractal
   and grey level co-occurrence matrix (GLCM) analysis methods in standard
   microscopy analysis of two histologically similar brain white mass
   regions that have different nerve fiber orientation. A total of 160
   digital micrographs of thionine-stained rat brain white mass were
   acquired using a Pro-MicroScan DEM-200 instrument. Eighty micrographs
   from the anterior corpus callosum and eighty from the anterior cingulum
   areas of the brain were analyzed. The micrographs were evaluated using
   the National Institutes of Health ImageJ software and its plugins. For
   each micrograph, seven parameters were calculated: angular second
   moment, inverse difference moment, GLCM contrast, GLCM correlation, GLCM
   variance, fractal dimension, and lacunarity. Using the Receiver
   operating characteristic analysis, the highest discriminatory value was
   determined for inverse difference moment (IDM) (area under the receiver
   operating characteristic (ROC) curve equaled 0.925, and for the
   criterion IDM <= 0.610 the sensitivity and specificity were 82.5 and
   87.5\%, respectively). Most of the other parameters also showed good
   sensitivity and specificity. The results indicate that GLCM and fractal
   analysis methods, when applied together in brain histology analysis, are
   highly capable of discriminating white mass structures that have
   different axonal orientation.",
journal = "Microscopy and Microanalysis",
title = "Application of Fractal and Grey Level Co-Occurrence Matrix Analysis in
 Evaluation of Brain Corpus Callosum and Cingulum Architecture",
number = "5",
volume = "20",
doi = "10.1017/S1431927614012811",
pages = "1373-1381"
}

Pantic, I., Dacic, S., Brkic, P., Lavrnja, I., Pantic, S., Jovanovic, T.,& Peković, S.. (2014). Application of Fractal and Grey Level Co-Occurrence Matrix Analysis in
 Evaluation of Brain Corpus Callosum and Cingulum Architecture. in Microscopy and Microanalysis, 20(5), 1373-1381.
https://doi.org/10.1017/S1431927614012811

Pantic I, Dacic S, Brkic P, Lavrnja I, Pantic S, Jovanovic T, Peković S. Application of Fractal and Grey Level Co-Occurrence Matrix Analysis in
 Evaluation of Brain Corpus Callosum and Cingulum Architecture. in Microscopy and Microanalysis. 2014;20(5):1373-1381.
doi:10.1017/S1431927614012811 .

Pantic, Igor, Dacic, Sanja, Brkic, Predrag, Lavrnja, Irena, Pantic, Senka, Jovanovic, Tomislav, Peković, Sanja, "Application of Fractal and Grey Level Co-Occurrence Matrix Analysis in
 Evaluation of Brain Corpus Callosum and Cingulum Architecture" in Microscopy and Microanalysis, 20, no. 5 (2014):1373-1381,
https://doi.org/10.1017/S1431927614012811 . .