TY  - JOUR
AU  - Zlatić, Nenad
AU  - Budečević, Sanja
AU  - Stanković, Milan
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5891
AB  - The shape–environment relationship in plants refers to the ways in which the physical
characteristics and structures of plants are influenced by their environment. Plants have evolved a
remarkable ability to adapt to their specific habitats, and their shape and form play a crucial role
in determining their survival and reproductive success. This study aimed to examine differences
in size and shape between morphological traits in mountain germander (Teucrium montanum L.)
from different geological substrates (calcareous and serpentinite). For this study, 400 individuals of
T. montanum from 20 populations (ten populations from the serpentinite and ten from the calcareous
substrate) were selected. Using the geometric morphometrics approach, it was shown that the degree
of phenotypic variation in the size and shape of the corolla, leaf, and stem of T. montanum depends
on the type of substrate. The main differences between the populations are the narrower part of the
lower lip of the corolla, the narrower leaf, and the wider central part of the vascular system stem
from serpentinite populations. The results of this study will contribute to a better understanding
of the morphological variability of T. montanum in relation to edaphic conditions. In addition, the
results confirm that certain morphological differences play an important role in the adaptive response
in relation to substrate composition, especially for substrates with increased metal content, such as
serpentinite. The shape–environment relationship in plants could define diversity and complexity in
plant life, and underscores the importance of shape as a key factor in their survival and success in
different habitats.
PB  - Basel: MDPI
T2  - Plants
T1  - Geological Substrate Effects on Teucrium montanum L. (Lamiaceae) Morphological Traits: Geometric Morphometrics Approach
IS  - 12
VL  - 12
DO  - 10.3390/plants12122381
SP  - 2381
ER  - 

@article{
author = "Zlatić, Nenad and Budečević, Sanja and Stanković, Milan",
year = "2023",
abstract = "The shape–environment relationship in plants refers to the ways in which the physical
characteristics and structures of plants are influenced by their environment. Plants have evolved a
remarkable ability to adapt to their specific habitats, and their shape and form play a crucial role
in determining their survival and reproductive success. This study aimed to examine differences
in size and shape between morphological traits in mountain germander (Teucrium montanum L.)
from different geological substrates (calcareous and serpentinite). For this study, 400 individuals of
T. montanum from 20 populations (ten populations from the serpentinite and ten from the calcareous
substrate) were selected. Using the geometric morphometrics approach, it was shown that the degree
of phenotypic variation in the size and shape of the corolla, leaf, and stem of T. montanum depends
on the type of substrate. The main differences between the populations are the narrower part of the
lower lip of the corolla, the narrower leaf, and the wider central part of the vascular system stem
from serpentinite populations. The results of this study will contribute to a better understanding
of the morphological variability of T. montanum in relation to edaphic conditions. In addition, the
results confirm that certain morphological differences play an important role in the adaptive response
in relation to substrate composition, especially for substrates with increased metal content, such as
serpentinite. The shape–environment relationship in plants could define diversity and complexity in
plant life, and underscores the importance of shape as a key factor in their survival and success in
different habitats.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "Geological Substrate Effects on Teucrium montanum L. (Lamiaceae) Morphological Traits: Geometric Morphometrics Approach",
number = "12",
volume = "12",
doi = "10.3390/plants12122381",
pages = "2381"
}

Zlatić, N., Budečević, S.,& Stanković, M.. (2023). Geological Substrate Effects on Teucrium montanum L. (Lamiaceae) Morphological Traits: Geometric Morphometrics Approach. in Plants
Basel: MDPI., 12(12), 2381.
https://doi.org/10.3390/plants12122381

Zlatić N, Budečević S, Stanković M. Geological Substrate Effects on Teucrium montanum L. (Lamiaceae) Morphological Traits: Geometric Morphometrics Approach. in Plants. 2023;12(12):2381.
doi:10.3390/plants12122381 .

Zlatić, Nenad, Budečević, Sanja, Stanković, Milan, "Geological Substrate Effects on Teucrium montanum L. (Lamiaceae) Morphological Traits: Geometric Morphometrics Approach" in Plants, 12, no. 12 (2023):2381,
https://doi.org/10.3390/plants12122381 . .

TY  - JOUR
AU  - Milutinović, Milena G.
AU  - Maksimović, Vuk M.
AU  - Cvetković, Danijela M.
AU  - Nikodijević, Danijela D.
AU  - Stanković, Milan S.
AU  - Pešić, Milica
AU  - Marković, Snežana D.
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0378874119303010?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3406
AB  - ETHNOPHARMACOLOGICAL RELEVANCE Teucrum chamaedrys L. is one of the known medicinal plants, useful for treatment of various health problems, especially digestive. In this study, we investigated methanol, ethyl-acetate and acetone extracts of T. chamaedrys in respect to their anticancer properties in SW480 colorectal cancer cells. MATERIALS AND METHODS Cytotoxicity and proapoptotic potential were assessed by MTT cell viability assay and AO/EB double staining. Molecular mechanisms of induced apoptosis were determined by monitoring Fas receptor protein expression through immunofluorescence, Caspase 8 and 9 activity, as well as concentrations of O2.- spectrophotometrically. Additionally, mRNA expression of biotransformation enzymes (CYP1A1, CYP1B1, GSTP1) and membrane transporters (MRP1 and MRP2) involved in drug resistance were investigated by qPCR method. Qualitative analysis of individual phenolic compounds was performed by reversed phase HPLC-MS analysis. RESULTS Methanol extract shows the best cytotoxicity and selectivity compared to ethyl-acetate and acetone extracts, mainly causing apoptosis of SW480 cells, without affecting normal HaCaT keratinocytes. The increased expression of Fas receptor protein and caspase 8 activity indicate that the death receptor-mediated pathway plays a crucial role in the observed apoptosis. The increased caspase 9 activity and O2.- concentration suggest that mitochondria are also involved in the apoptosis. T. chamaedrys methanol extract inhibits mRNA expression of CYP1A1, CYP1B1, GSTP1, MRP1 and MRP2 in SW480 cells. CONCLUSIONS Induction of apoptosis and inhibition of CYP1A1, CYP1B1, GSTP1, MRP1 and MRP2 mRNA expression implies that T. chamaedrys can serve as a valuable source of bioactive compounds as dietary supplements or selective anticancer agents, with the ability to induce apoptosis and modulate drug resistance in colorectal cancer cells.
T2  - Journal of Ethnopharmacology
T1  - Potential of Teucrium chamaedrys L. to modulate apoptosis and biotransformation in colorectal carcinoma cells.
VL  - 240
DO  - 10.1016/j.jep.2019.111951
SP  - 111951
ER  - 

@article{
author = "Milutinović, Milena G. and Maksimović, Vuk M. and Cvetković, Danijela M. and Nikodijević, Danijela D. and Stanković, Milan S. and Pešić, Milica and Marković, Snežana D.",
year = "2019",
abstract = "ETHNOPHARMACOLOGICAL RELEVANCE Teucrum chamaedrys L. is one of the known medicinal plants, useful for treatment of various health problems, especially digestive. In this study, we investigated methanol, ethyl-acetate and acetone extracts of T. chamaedrys in respect to their anticancer properties in SW480 colorectal cancer cells. MATERIALS AND METHODS Cytotoxicity and proapoptotic potential were assessed by MTT cell viability assay and AO/EB double staining. Molecular mechanisms of induced apoptosis were determined by monitoring Fas receptor protein expression through immunofluorescence, Caspase 8 and 9 activity, as well as concentrations of O2.- spectrophotometrically. Additionally, mRNA expression of biotransformation enzymes (CYP1A1, CYP1B1, GSTP1) and membrane transporters (MRP1 and MRP2) involved in drug resistance were investigated by qPCR method. Qualitative analysis of individual phenolic compounds was performed by reversed phase HPLC-MS analysis. RESULTS Methanol extract shows the best cytotoxicity and selectivity compared to ethyl-acetate and acetone extracts, mainly causing apoptosis of SW480 cells, without affecting normal HaCaT keratinocytes. The increased expression of Fas receptor protein and caspase 8 activity indicate that the death receptor-mediated pathway plays a crucial role in the observed apoptosis. The increased caspase 9 activity and O2.- concentration suggest that mitochondria are also involved in the apoptosis. T. chamaedrys methanol extract inhibits mRNA expression of CYP1A1, CYP1B1, GSTP1, MRP1 and MRP2 in SW480 cells. CONCLUSIONS Induction of apoptosis and inhibition of CYP1A1, CYP1B1, GSTP1, MRP1 and MRP2 mRNA expression implies that T. chamaedrys can serve as a valuable source of bioactive compounds as dietary supplements or selective anticancer agents, with the ability to induce apoptosis and modulate drug resistance in colorectal cancer cells.",
journal = "Journal of Ethnopharmacology",
title = "Potential of Teucrium chamaedrys L. to modulate apoptosis and biotransformation in colorectal carcinoma cells.",
volume = "240",
doi = "10.1016/j.jep.2019.111951",
pages = "111951"
}

Milutinović, M. G., Maksimović, V. M., Cvetković, D. M., Nikodijević, D. D., Stanković, M. S., Pešić, M.,& Marković, S. D.. (2019). Potential of Teucrium chamaedrys L. to modulate apoptosis and biotransformation in colorectal carcinoma cells.. in Journal of Ethnopharmacology, 240, 111951.
https://doi.org/10.1016/j.jep.2019.111951

Milutinović MG, Maksimović VM, Cvetković DM, Nikodijević DD, Stanković MS, Pešić M, Marković SD. Potential of Teucrium chamaedrys L. to modulate apoptosis and biotransformation in colorectal carcinoma cells.. in Journal of Ethnopharmacology. 2019;240:111951.
doi:10.1016/j.jep.2019.111951 .

Milutinović, Milena G., Maksimović, Vuk M., Cvetković, Danijela M., Nikodijević, Danijela D., Stanković, Milan S., Pešić, Milica, Marković, Snežana D., "Potential of Teucrium chamaedrys L. to modulate apoptosis and biotransformation in colorectal carcinoma cells." in Journal of Ethnopharmacology, 240 (2019):111951,
https://doi.org/10.1016/j.jep.2019.111951 . .

TY  - JOUR
AU  - Bošnjak-Neumüller, Jasna
AU  - Radaković, Milena
AU  - Djelić, Ninoslav
AU  - Vuković-Gačić, Branka
AU  - Stevanović, Zora Dajić
AU  - Kolarević, Stoimir
AU  - Mišić, Danijela
AU  - Stanković, Milan
AU  - Knežević-Vukčević, Jelena
AU  - Spremo-Potparević, Biljana
AU  - Stanimirović, Zoran
PY  - 2017
UR  - http://www.ncbi.nlm.nih.gov/pubmed/28650332
UR  - http://www.pjps.pk/wp-content/uploads/pdfs/30/2/Supplementary/13-SUP-652.pdf
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3537
AB  - The success of antioxidant therapy in hyperthyroidism implies that disease is mediated by oxidative stress, which is known as one of the causing agents of ageing, degenerative diseases, and cancer. The main objective of our study was to determine possible protective effects of methanolic extract of N. rtanjensis in triiodothyronine (T3)-induced DNA breaks of human lymphocytes under in vitro conditions, based upon plant antioxidant capacity related to its phytochemical profile, mainly its polyphenolic complex. The total phenolic and flavonoid content and the antioxidant activity using in vitro 1,1-dyphenyl-2- picrylhydrazyl reagent (DPPH) was determined in methanolic extracts of plant leaves and flowers. The phenolic compound content of 62.73±1.80mg of GaA/g, exhibited solid antioxidant activity (IC50= 112.59±0.95μg/ml). The antigenotoxic activity of 0.2, 0.5 and 1.0mg/ml N. rtanjensis methanol extracts mixture with 100µM of T3 was studied in human lymphocytes in vitro using the Comet assay. It is supposed that the antigenotoxicity of N. rtanjensis methanol extracts was caused by high presence of chlorogenic acid, rosmarinic acid and rutin, all known as efficient antioxidant bioactive compounds, which were determined by ultrahigh-pressure liquid chromatograph with MS/MS Mass Spectroscopy (UHPLC/-HESI-MS / MS).
T2  - Pakistan Journal of Pharmaceutical Sciences
T1  - Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition, antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes.
IS  - 2(Suppl.)
VL  - 30
SP  - 625
EP  - 634
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4504
ER  - 

@article{
author = "Bošnjak-Neumüller, Jasna and Radaković, Milena and Djelić, Ninoslav and Vuković-Gačić, Branka and Stevanović, Zora Dajić and Kolarević, Stoimir and Mišić, Danijela and Stanković, Milan and Knežević-Vukčević, Jelena and Spremo-Potparević, Biljana and Stanimirović, Zoran",
year = "2017",
abstract = "The success of antioxidant therapy in hyperthyroidism implies that disease is mediated by oxidative stress, which is known as one of the causing agents of ageing, degenerative diseases, and cancer. The main objective of our study was to determine possible protective effects of methanolic extract of N. rtanjensis in triiodothyronine (T3)-induced DNA breaks of human lymphocytes under in vitro conditions, based upon plant antioxidant capacity related to its phytochemical profile, mainly its polyphenolic complex. The total phenolic and flavonoid content and the antioxidant activity using in vitro 1,1-dyphenyl-2- picrylhydrazyl reagent (DPPH) was determined in methanolic extracts of plant leaves and flowers. The phenolic compound content of 62.73±1.80mg of GaA/g, exhibited solid antioxidant activity (IC50= 112.59±0.95μg/ml). The antigenotoxic activity of 0.2, 0.5 and 1.0mg/ml N. rtanjensis methanol extracts mixture with 100µM of T3 was studied in human lymphocytes in vitro using the Comet assay. It is supposed that the antigenotoxicity of N. rtanjensis methanol extracts was caused by high presence of chlorogenic acid, rosmarinic acid and rutin, all known as efficient antioxidant bioactive compounds, which were determined by ultrahigh-pressure liquid chromatograph with MS/MS Mass Spectroscopy (UHPLC/-HESI-MS / MS).",
journal = "Pakistan Journal of Pharmaceutical Sciences",
title = "Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition, antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes.",
number = "2(Suppl.)",
volume = "30",
pages = "625-634",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4504"
}

Bošnjak-Neumüller, J., Radaković, M., Djelić, N., Vuković-Gačić, B., Stevanović, Z. D., Kolarević, S., Mišić, D., Stanković, M., Knežević-Vukčević, J., Spremo-Potparević, B.,& Stanimirović, Z.. (2017). Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition, antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes.. in Pakistan Journal of Pharmaceutical Sciences, 30(2(Suppl.)), 625-634.
https://hdl.handle.net/21.15107/rcub_agrospace_4504

Bošnjak-Neumüller J, Radaković M, Djelić N, Vuković-Gačić B, Stevanović ZD, Kolarević S, Mišić D, Stanković M, Knežević-Vukčević J, Spremo-Potparević B, Stanimirović Z. Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition, antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes.. in Pakistan Journal of Pharmaceutical Sciences. 2017;30(2(Suppl.)):625-634.
https://hdl.handle.net/21.15107/rcub_agrospace_4504 .

Bošnjak-Neumüller, Jasna, Radaković, Milena, Djelić, Ninoslav, Vuković-Gačić, Branka, Stevanović, Zora Dajić, Kolarević, Stoimir, Mišić, Danijela, Stanković, Milan, Knežević-Vukčević, Jelena, Spremo-Potparević, Biljana, Stanimirović, Zoran, "Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition, antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes." in Pakistan Journal of Pharmaceutical Sciences, 30, no. 2(Suppl.) (2017):625-634,
https://hdl.handle.net/21.15107/rcub_agrospace_4504 .