TY  - JOUR
AU  - Tusevski, Oliver
AU  - Vinterhalter, Branka
AU  - Krstić Milošević, Dijana
AU  - Soković, Marina
AU  - Ćirić, Ana
AU  - Vinterhalter, Dragan
AU  - Zdravković-Korać, Snežana
AU  - Petreska Stanoeva, Jasmina
AU  - Stefova, Marina
AU  - Gadzovska Simic, Sonja
PY  - 2017
UR  - http://link.springer.com/10.1007/s11240-016-1136-9
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2688
AB  - Three hairy root clones of Hypericum perforatum (HR 2, HR 15 and HR 27) transformed with Agrobacterium rhizogenes A4M70GUS and their corresponding regenerated shoot culture clones (HRRS) were compared for differences in growth, production of phenolic compounds, antioxidant and antimicrobial activities. Transgenic clones were selected on the basis of morphological evaluation, genetic and molecular analyses. The clone HR 2 had the highest biomass accumulation, while HR 27 showed the highest shoot regeneration potential. The total phenolics and flavan-3-ols were enhanced in all tested transgenic cultures, while total flavonoids and hypericins were augmented in HRRS clones compared to non-transformed shoots. The HRRS clones produced substantial amounts of chlorogenic acid and 3-p-coumaroylquinic acid. Regarding the flavonoids, they produced significant contents of luteolin hexoside (HRRS 2), quercitrin and quercetin (HRRS 15) and isoquercetin (HRRS 27), while HR 2 and 15 accumulated 4-O-methylkaempferol-O-hexoside and quercetin 6-C-glucoside, respectively. The HR 15 was promising for the production of catechin and procyanidin derivatives and together with its HRRS clone exhibited a high potential for hyperforin and adhyperforin production. All identified naphtodianthrones were confirmed in HRRS 2 and 15 clones. Among xanthones, mangiferin was found as the major compound in HRRS, while trihydroxy-1-metoxy-C-prenyl xanthone was dominant in HR clones. Antimicrobial activity of transgenic cultures revealed that HRRS 15 strongly inhibited the growth of Bacillus cereus, Micrococcus flavus, Pseudomonas aeruginosa and Escherichia coli. Altogether, H. perforatum HR and HRRS cultures could be proposed as promising experimental systems for enhanced production of phenolic compounds with antioxidant and antibacterial properties.
T2  - Plant Cell, Tissue and Organ Culture (PCTOC)
T1  - Production of phenolic compounds, antioxidant and antimicrobial activities in hairy root and shoot cultures of Hypericum perforatum L.
IS  - 3
VL  - 128
DO  - 10.1007/s11240-016-1136-9
SP  - 589
EP  - 605
ER  - 

@article{
author = "Tusevski, Oliver and Vinterhalter, Branka and Krstić Milošević, Dijana and Soković, Marina and Ćirić, Ana and Vinterhalter, Dragan and Zdravković-Korać, Snežana and Petreska Stanoeva, Jasmina and Stefova, Marina and Gadzovska Simic, Sonja",
year = "2017",
abstract = "Three hairy root clones of Hypericum perforatum (HR 2, HR 15 and HR 27) transformed with Agrobacterium rhizogenes A4M70GUS and their corresponding regenerated shoot culture clones (HRRS) were compared for differences in growth, production of phenolic compounds, antioxidant and antimicrobial activities. Transgenic clones were selected on the basis of morphological evaluation, genetic and molecular analyses. The clone HR 2 had the highest biomass accumulation, while HR 27 showed the highest shoot regeneration potential. The total phenolics and flavan-3-ols were enhanced in all tested transgenic cultures, while total flavonoids and hypericins were augmented in HRRS clones compared to non-transformed shoots. The HRRS clones produced substantial amounts of chlorogenic acid and 3-p-coumaroylquinic acid. Regarding the flavonoids, they produced significant contents of luteolin hexoside (HRRS 2), quercitrin and quercetin (HRRS 15) and isoquercetin (HRRS 27), while HR 2 and 15 accumulated 4-O-methylkaempferol-O-hexoside and quercetin 6-C-glucoside, respectively. The HR 15 was promising for the production of catechin and procyanidin derivatives and together with its HRRS clone exhibited a high potential for hyperforin and adhyperforin production. All identified naphtodianthrones were confirmed in HRRS 2 and 15 clones. Among xanthones, mangiferin was found as the major compound in HRRS, while trihydroxy-1-metoxy-C-prenyl xanthone was dominant in HR clones. Antimicrobial activity of transgenic cultures revealed that HRRS 15 strongly inhibited the growth of Bacillus cereus, Micrococcus flavus, Pseudomonas aeruginosa and Escherichia coli. Altogether, H. perforatum HR and HRRS cultures could be proposed as promising experimental systems for enhanced production of phenolic compounds with antioxidant and antibacterial properties.",
journal = "Plant Cell, Tissue and Organ Culture (PCTOC)",
title = "Production of phenolic compounds, antioxidant and antimicrobial activities in hairy root and shoot cultures of Hypericum perforatum L.",
number = "3",
volume = "128",
doi = "10.1007/s11240-016-1136-9",
pages = "589-605"
}

Tusevski, O., Vinterhalter, B., Krstić Milošević, D., Soković, M., Ćirić, A., Vinterhalter, D., Zdravković-Korać, S., Petreska Stanoeva, J., Stefova, M.,& Gadzovska Simic, S.. (2017). Production of phenolic compounds, antioxidant and antimicrobial activities in hairy root and shoot cultures of Hypericum perforatum L.. in Plant Cell, Tissue and Organ Culture (PCTOC), 128(3), 589-605.
https://doi.org/10.1007/s11240-016-1136-9

Tusevski O, Vinterhalter B, Krstić Milošević D, Soković M, Ćirić A, Vinterhalter D, Zdravković-Korać S, Petreska Stanoeva J, Stefova M, Gadzovska Simic S. Production of phenolic compounds, antioxidant and antimicrobial activities in hairy root and shoot cultures of Hypericum perforatum L.. in Plant Cell, Tissue and Organ Culture (PCTOC). 2017;128(3):589-605.
doi:10.1007/s11240-016-1136-9 .

Tusevski, Oliver, Vinterhalter, Branka, Krstić Milošević, Dijana, Soković, Marina, Ćirić, Ana, Vinterhalter, Dragan, Zdravković-Korać, Snežana, Petreska Stanoeva, Jasmina, Stefova, Marina, Gadzovska Simic, Sonja, "Production of phenolic compounds, antioxidant and antimicrobial activities in hairy root and shoot cultures of Hypericum perforatum L." in Plant Cell, Tissue and Organ Culture (PCTOC), 128, no. 3 (2017):589-605,
https://doi.org/10.1007/s11240-016-1136-9 . .