TY  - CONF
AU  - Belić, Maja
AU  - Anačkov, Goran
AU  - Ćalić, Dušica
AU  - Devrnja, Nina
AU  - Igić, Ružica
AU  - Milojević, Jelena
AU  - Zdravković-Korać, Snežana
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4325
AB  - A. atropurpureum is a natural rarity of Serbia, growing only in the area of Vojvodina. The vulnerability of its habitat by fragmentation and frequent and uncontrolled chemical treatment of
surrounding agrarian areas, affects its status and the impoverishment of natural populations.
Therefore, establishing a protocol for efficient de novo regeneration of this species for ex situ conservation was the aim of the present study. For callus induction, the apical root sections of axenic
seedlings were cultivated on medium supplemented with 5 μM 2,4 D + 5 μM BA for 8 weeks. The
obtained calli were friable, pale beige, without regeneration capacity. However, within these calli, a
compact yellowish callus formed, and this type of callus had the capacity for bud formation when
cultivated on media containing 0, 1, 5 or 10 μM TDZ, Kin or BA for 8 weeks. Calli cultivated on medium supplemented with 10 μM Kin exhibited the highest bud forming capacity, with the lowest
level of hyperhydricity and albinism. Shoot bunches were further hardened on plant growth regulator-free medium for 8 weeks, and then single plants were detached and subcultivated on media
with 0, 1, 5 or 10 μM GA3 and grown at 12 °C, for bulblet induction. All plants formed bulblets, but
bulblets cultivated on GA3-containing media multiplied by setting up to 10 secondary bulblets.
Obtained bulblets are suitable propagules, as they easily develop into plants. This protocol is convenient for clonal propagation of this endangered plant species, as well as for other applications
in its research and improvement.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting)
T1  - Bud regeneration from root-tips of Allium atropurpureum Waldst. & Kit.
SP  - 30
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4325
ER  - 

@conference{
author = "Belić, Maja and Anačkov, Goran and Ćalić, Dušica and Devrnja, Nina and Igić, Ružica and Milojević, Jelena and Zdravković-Korać, Snežana",
year = "2018",
abstract = "A. atropurpureum is a natural rarity of Serbia, growing only in the area of Vojvodina. The vulnerability of its habitat by fragmentation and frequent and uncontrolled chemical treatment of
surrounding agrarian areas, affects its status and the impoverishment of natural populations.
Therefore, establishing a protocol for efficient de novo regeneration of this species for ex situ conservation was the aim of the present study. For callus induction, the apical root sections of axenic
seedlings were cultivated on medium supplemented with 5 μM 2,4 D + 5 μM BA for 8 weeks. The
obtained calli were friable, pale beige, without regeneration capacity. However, within these calli, a
compact yellowish callus formed, and this type of callus had the capacity for bud formation when
cultivated on media containing 0, 1, 5 or 10 μM TDZ, Kin or BA for 8 weeks. Calli cultivated on medium supplemented with 10 μM Kin exhibited the highest bud forming capacity, with the lowest
level of hyperhydricity and albinism. Shoot bunches were further hardened on plant growth regulator-free medium for 8 weeks, and then single plants were detached and subcultivated on media
with 0, 1, 5 or 10 μM GA3 and grown at 12 °C, for bulblet induction. All plants formed bulblets, but
bulblets cultivated on GA3-containing media multiplied by setting up to 10 secondary bulblets.
Obtained bulblets are suitable propagules, as they easily develop into plants. This protocol is convenient for clonal propagation of this endangered plant species, as well as for other applications
in its research and improvement.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting)",
title = "Bud regeneration from root-tips of Allium atropurpureum Waldst. & Kit.",
pages = "30",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4325"
}

Belić, M., Anačkov, G., Ćalić, D., Devrnja, N., Igić, R., Milojević, J.,& Zdravković-Korać, S.. (2018). Bud regeneration from root-tips of Allium atropurpureum Waldst. & Kit.. in 3rd International Conference on Plant Biology (22nd SPPS Meeting)
Belgrade: Serbian Plant Physiology Society., 30.
https://hdl.handle.net/21.15107/rcub_ibiss_4325

Belić M, Anačkov G, Ćalić D, Devrnja N, Igić R, Milojević J, Zdravković-Korać S. Bud regeneration from root-tips of Allium atropurpureum Waldst. & Kit.. in 3rd International Conference on Plant Biology (22nd SPPS Meeting). 2018;:30.
https://hdl.handle.net/21.15107/rcub_ibiss_4325 .

Belić, Maja, Anačkov, Goran, Ćalić, Dušica, Devrnja, Nina, Igić, Ružica, Milojević, Jelena, Zdravković-Korać, Snežana, "Bud regeneration from root-tips of Allium atropurpureum Waldst. & Kit." in 3rd International Conference on Plant Biology (22nd SPPS Meeting) (2018):30,
https://hdl.handle.net/21.15107/rcub_ibiss_4325 .