TY  - JOUR
AU  - Kaluđerović, Milena
AU  - Krajnović, Tamara
AU  - Maksimović-Ivanić, Danijela
AU  - Graf, Hans-Ludwig
AU  - Mijatović, Sanja
PY  - 2017
UR  - http://www.mdpi.com/2079-6412/7/7/102
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3307
AB  - A primary goal in modern surface modification technology of dental implants is to achieve biocompatible surfaces with rapid but controlled healing which also allow health and longevity of implants. In order to realize all, understanding of osseointegration phenomena is crucial. Although Ti-SLA, Ti-SLActive and TiZr-SLActive surfaces have been successfully used in clinical implantology and were shown to notably reduce the primary healing time, available in vitro studies are sparse and do not concern or explore the mechanism(s) involved in human osteoblast behavior on these surfaces. Ti-SLA, Ti-SLActive, TiZr-SLActive, Ti cp, Ticer and Cercon surfaces were used. Osteoblast proliferation, cell cluster formation, morphological changes, induction of autophagy, nitric oxide (NO), reactive oxygen species/reactive nitrogen species (ROS/RNS) formation, osteocalcin (OC), bone sialoprotein (BSP) and collagen type I (Col-1) affected by various surfaces were analyzed. These surfaces induced formation of mature osteoblasts caused by elevated oxidative stress (ROS) followed by overexpression of osteoblast maturation key molecule (NO), with different intensity however. These mature osteoblasts induced upregulation of OC, BSP and Col-1, activating PI3/Akt signalling pathway resulting in autophagy, known as an important process in differentiation of osteoblast cells. Additional distinctive subpopulation identified on Ticer, Ti-SLA (after 5 days), Ti-SLActive and TiZr-SLActive surfaces (after 2 days) were forming cell clusters, essential for bone noduli formation and mineralisation. The results suggest that Ti- and TiZr-SLActive possess advanced properties in comparison with Ticer and Ti-SLA manifested as accelerated osteoblast differentiation. These effects could explain already known fast osseointegration of these surfaces in vivo.
T2  - Coatings
T1  - Ti-SLActive and TiZr-SLActive Dental Implant Surfaces Promote Fast Osteoblast Differentiation
IS  - 7
VL  - 7
DO  - 10.3390/coatings7070102
SP  - 102
ER  - 

@article{
author = "Kaluđerović, Milena and Krajnović, Tamara and Maksimović-Ivanić, Danijela and Graf, Hans-Ludwig and Mijatović, Sanja",
year = "2017",
abstract = "A primary goal in modern surface modification technology of dental implants is to achieve biocompatible surfaces with rapid but controlled healing which also allow health and longevity of implants. In order to realize all, understanding of osseointegration phenomena is crucial. Although Ti-SLA, Ti-SLActive and TiZr-SLActive surfaces have been successfully used in clinical implantology and were shown to notably reduce the primary healing time, available in vitro studies are sparse and do not concern or explore the mechanism(s) involved in human osteoblast behavior on these surfaces. Ti-SLA, Ti-SLActive, TiZr-SLActive, Ti cp, Ticer and Cercon surfaces were used. Osteoblast proliferation, cell cluster formation, morphological changes, induction of autophagy, nitric oxide (NO), reactive oxygen species/reactive nitrogen species (ROS/RNS) formation, osteocalcin (OC), bone sialoprotein (BSP) and collagen type I (Col-1) affected by various surfaces were analyzed. These surfaces induced formation of mature osteoblasts caused by elevated oxidative stress (ROS) followed by overexpression of osteoblast maturation key molecule (NO), with different intensity however. These mature osteoblasts induced upregulation of OC, BSP and Col-1, activating PI3/Akt signalling pathway resulting in autophagy, known as an important process in differentiation of osteoblast cells. Additional distinctive subpopulation identified on Ticer, Ti-SLA (after 5 days), Ti-SLActive and TiZr-SLActive surfaces (after 2 days) were forming cell clusters, essential for bone noduli formation and mineralisation. The results suggest that Ti- and TiZr-SLActive possess advanced properties in comparison with Ticer and Ti-SLA manifested as accelerated osteoblast differentiation. These effects could explain already known fast osseointegration of these surfaces in vivo.",
journal = "Coatings",
title = "Ti-SLActive and TiZr-SLActive Dental Implant Surfaces Promote Fast Osteoblast Differentiation",
number = "7",
volume = "7",
doi = "10.3390/coatings7070102",
pages = "102"
}

Kaluđerović, M., Krajnović, T., Maksimović-Ivanić, D., Graf, H.,& Mijatović, S.. (2017). Ti-SLActive and TiZr-SLActive Dental Implant Surfaces Promote Fast Osteoblast Differentiation. in Coatings, 7(7), 102.
https://doi.org/10.3390/coatings7070102

Kaluđerović M, Krajnović T, Maksimović-Ivanić D, Graf H, Mijatović S. Ti-SLActive and TiZr-SLActive Dental Implant Surfaces Promote Fast Osteoblast Differentiation. in Coatings. 2017;7(7):102.
doi:10.3390/coatings7070102 .

Kaluđerović, Milena, Krajnović, Tamara, Maksimović-Ivanić, Danijela, Graf, Hans-Ludwig, Mijatović, Sanja, "Ti-SLActive and TiZr-SLActive Dental Implant Surfaces Promote Fast Osteoblast Differentiation" in Coatings, 7, no. 7 (2017):102,
https://doi.org/10.3390/coatings7070102 . .

TY  - JOUR
AU  - Kaluderovic, Milena R.
AU  - Mojić, Marija
AU  - Schreckenbach, Joachim P.
AU  - Maksimović-Ivanić, Danijela
AU  - Graf, Hans-Ludwig
AU  - Mijatović, Sanja
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2251
AB  - Autophagy plays an important role in embryogenesis, for the maintenance
   of tissue homeostasis and the elimination of damaged subcellular
   structures. Furthermore, autophagy could be a mode of physiological cell
   death and also be implicated in cell differentiation. Thus, we
   hypothesized that autophagy may have an impact on the differentiation of
   osteoblast cells influenced by various titanium-based surfaces.
   Interactions between smooth, commercially available pure titanium (Ti
   cp), rough Ticer, acid-etched Ti cp (SS) and M1-M3 (comprised of the
   monoclinic phase of sodium-titanium oxides and rutile; M2 contains
   amorphous calcium phosphates) and human osteoblast cells were
   investigated. Immunofluorescent staining was used for detecting
   autophagy, cell cluster formation and collagen type I (Col-1)
   expression. Flow cytometry was employed to identify autophagy, the
   production of endogenous nitric oxide (NO) and the size and granularity
   of the cells. Rough surfaces caused osteoblast differentiation via the
   autophagic-dependent PI3/Akt signalling pathway. These surfaces induced
   the formation of discrete populations of large, granular cells, i.e.
   mature osteoblasts. In addition, M1-M3 provoked the development of a
   third population of small, granular cells, responsible for cell cluster
   formation, which are important for the formation of bone noduli and
   mineralisation. The same surfaces induced faster osteoblast maturation
   and enhanced NO production, a hallmark of the already mentioned
   processes. Neither the mature osteoblasts nor the small cells appeared
   after the inhibition of autophagy. Inhibition of autophagy also
   prevented cell cluster formation. We demonstrate that autophagy plays an
   essential role in the osteoblast differentiation on titanium-based
   surfaces with rough topography. (C) 2015 S. Karger AG, Basel
T2  - Cells Tissues Organs
T1  - A Key Role of Autophagy in Osteoblast Differentiation on Titanium-Based
 Dental Implants
IS  - 3-4
VL  - 200
DO  - 10.1159/000434625
SP  - 265
EP  - 277
ER  - 

@article{
author = "Kaluderovic, Milena R. and Mojić, Marija and Schreckenbach, Joachim P. and Maksimović-Ivanić, Danijela and Graf, Hans-Ludwig and Mijatović, Sanja",
year = "2014",
abstract = "Autophagy plays an important role in embryogenesis, for the maintenance
   of tissue homeostasis and the elimination of damaged subcellular
   structures. Furthermore, autophagy could be a mode of physiological cell
   death and also be implicated in cell differentiation. Thus, we
   hypothesized that autophagy may have an impact on the differentiation of
   osteoblast cells influenced by various titanium-based surfaces.
   Interactions between smooth, commercially available pure titanium (Ti
   cp), rough Ticer, acid-etched Ti cp (SS) and M1-M3 (comprised of the
   monoclinic phase of sodium-titanium oxides and rutile; M2 contains
   amorphous calcium phosphates) and human osteoblast cells were
   investigated. Immunofluorescent staining was used for detecting
   autophagy, cell cluster formation and collagen type I (Col-1)
   expression. Flow cytometry was employed to identify autophagy, the
   production of endogenous nitric oxide (NO) and the size and granularity
   of the cells. Rough surfaces caused osteoblast differentiation via the
   autophagic-dependent PI3/Akt signalling pathway. These surfaces induced
   the formation of discrete populations of large, granular cells, i.e.
   mature osteoblasts. In addition, M1-M3 provoked the development of a
   third population of small, granular cells, responsible for cell cluster
   formation, which are important for the formation of bone noduli and
   mineralisation. The same surfaces induced faster osteoblast maturation
   and enhanced NO production, a hallmark of the already mentioned
   processes. Neither the mature osteoblasts nor the small cells appeared
   after the inhibition of autophagy. Inhibition of autophagy also
   prevented cell cluster formation. We demonstrate that autophagy plays an
   essential role in the osteoblast differentiation on titanium-based
   surfaces with rough topography. (C) 2015 S. Karger AG, Basel",
journal = "Cells Tissues Organs",
title = "A Key Role of Autophagy in Osteoblast Differentiation on Titanium-Based
 Dental Implants",
number = "3-4",
volume = "200",
doi = "10.1159/000434625",
pages = "265-277"
}

Kaluderovic, M. R., Mojić, M., Schreckenbach, J. P., Maksimović-Ivanić, D., Graf, H.,& Mijatović, S.. (2014). A Key Role of Autophagy in Osteoblast Differentiation on Titanium-Based
 Dental Implants. in Cells Tissues Organs, 200(3-4), 265-277.
https://doi.org/10.1159/000434625

Kaluderovic MR, Mojić M, Schreckenbach JP, Maksimović-Ivanić D, Graf H, Mijatović S. A Key Role of Autophagy in Osteoblast Differentiation on Titanium-Based
 Dental Implants. in Cells Tissues Organs. 2014;200(3-4):265-277.
doi:10.1159/000434625 .

Kaluderovic, Milena R., Mojić, Marija, Schreckenbach, Joachim P., Maksimović-Ivanić, Danijela, Graf, Hans-Ludwig, Mijatović, Sanja, "A Key Role of Autophagy in Osteoblast Differentiation on Titanium-Based
 Dental Implants" in Cells Tissues Organs, 200, no. 3-4 (2014):265-277,
https://doi.org/10.1159/000434625 . .