TY  - JOUR
AU  - Filipović, Biljana
AU  - Skorić, Marijana
AU  - Gašić, Uroš
AU  - Dragićević, Milan
AU  - Božunović, Jelena
AU  - Matekalo, Dragana
AU  - Nestorović Živković, Jasmina
AU  - Banjanac, Tijana
AU  - Šiler, Branislav
AU  - Bohanec, Borut
AU  - Milutinović, Milica
AU  - Mišić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5000
AB  - Centaurium erythraea, used as medical plant from the earliest times, is an immense depot of quite rare bioactive compounds. Secoiridoids (sweroside, swertiamarin, and gentiopicrin) and xanthones (methylbellidifolin and decussatin) are predominant bioactive compounds in C. erythraea. The present study aims at providing new insights into how the content of these bioactive principles can be related with plant ploidy level by characterizing possible differences in their biosynthesis and accumulation between diploid and tetraploid genotypes from both spatial and temporal aspects. In general, shoots are determined as the major site of secoiridoids’ and xanthones’ accumulation, whose ratio vary during the development. Genes involved in iridoid and xanthone metabolic pathways were found to be coordinately regulated at the transcriptional level both during the development and among organs. Biosynthetic gene expression levels were found highly correlated with the content of major compounds from these two classes. Diversification in chemical profiles between tetraploid and diploid genotypes may result from the expression difference between homologous loci correspondent to several key biosynthetic genes, which trigger changes in the two metabolic routes. Thus, enhanced expression of genes coding for geraniol synthase (GES), 8-hydroxygeraniol oxidoreductase (8HGO), and 7-deoxyloganic acid hydrolase (7DLH2) is strongly associated with intensive production of iridoids. Interestingly, transcript levels of beta-glucosidase (CebGLU), a candidate to catalyze the first step in the secoiridoid catabolism, is significantly positively correlated with the content of major secoiridoids. Elevated expression of genes coding for benzophenone synthase (BS) and 3-hydroxybenzoate:CoA ligase (3HBL) appear to account for enhanced production of hexa-substituted xanthones. Regarding content of iridoids and xanthones, a diploid genotype appeared to be more productive than a tetraploid genotype under controlled in vitro conditions, therewithal displaying significantly higher biomass.
PB  - Amsterdam : Elsevier
T2  - Industrial Crops and Products
T1  - Spatial and temporal patterns of secoiridoid and xanthone biosynthetic pathways during early development of Centaurium erythraea Rafn, as altered by ploidy level
VL  - 186
DO  - 10.1016/j.indcrop.2022.115146
SP  - 115146
ER  - 

@article{
author = "Filipović, Biljana and Skorić, Marijana and Gašić, Uroš and Dragićević, Milan and Božunović, Jelena and Matekalo, Dragana and Nestorović Živković, Jasmina and Banjanac, Tijana and Šiler, Branislav and Bohanec, Borut and Milutinović, Milica and Mišić, Danijela",
year = "2022",
abstract = "Centaurium erythraea, used as medical plant from the earliest times, is an immense depot of quite rare bioactive compounds. Secoiridoids (sweroside, swertiamarin, and gentiopicrin) and xanthones (methylbellidifolin and decussatin) are predominant bioactive compounds in C. erythraea. The present study aims at providing new insights into how the content of these bioactive principles can be related with plant ploidy level by characterizing possible differences in their biosynthesis and accumulation between diploid and tetraploid genotypes from both spatial and temporal aspects. In general, shoots are determined as the major site of secoiridoids’ and xanthones’ accumulation, whose ratio vary during the development. Genes involved in iridoid and xanthone metabolic pathways were found to be coordinately regulated at the transcriptional level both during the development and among organs. Biosynthetic gene expression levels were found highly correlated with the content of major compounds from these two classes. Diversification in chemical profiles between tetraploid and diploid genotypes may result from the expression difference between homologous loci correspondent to several key biosynthetic genes, which trigger changes in the two metabolic routes. Thus, enhanced expression of genes coding for geraniol synthase (GES), 8-hydroxygeraniol oxidoreductase (8HGO), and 7-deoxyloganic acid hydrolase (7DLH2) is strongly associated with intensive production of iridoids. Interestingly, transcript levels of beta-glucosidase (CebGLU), a candidate to catalyze the first step in the secoiridoid catabolism, is significantly positively correlated with the content of major secoiridoids. Elevated expression of genes coding for benzophenone synthase (BS) and 3-hydroxybenzoate:CoA ligase (3HBL) appear to account for enhanced production of hexa-substituted xanthones. Regarding content of iridoids and xanthones, a diploid genotype appeared to be more productive than a tetraploid genotype under controlled in vitro conditions, therewithal displaying significantly higher biomass.",
publisher = "Amsterdam : Elsevier",
journal = "Industrial Crops and Products",
title = "Spatial and temporal patterns of secoiridoid and xanthone biosynthetic pathways during early development of Centaurium erythraea Rafn, as altered by ploidy level",
volume = "186",
doi = "10.1016/j.indcrop.2022.115146",
pages = "115146"
}

Filipović, B., Skorić, M., Gašić, U., Dragićević, M., Božunović, J., Matekalo, D., Nestorović Živković, J., Banjanac, T., Šiler, B., Bohanec, B., Milutinović, M.,& Mišić, D.. (2022). Spatial and temporal patterns of secoiridoid and xanthone biosynthetic pathways during early development of Centaurium erythraea Rafn, as altered by ploidy level. in Industrial Crops and Products
Amsterdam : Elsevier., 186, 115146.
https://doi.org/10.1016/j.indcrop.2022.115146

Filipović B, Skorić M, Gašić U, Dragićević M, Božunović J, Matekalo D, Nestorović Živković J, Banjanac T, Šiler B, Bohanec B, Milutinović M, Mišić D. Spatial and temporal patterns of secoiridoid and xanthone biosynthetic pathways during early development of Centaurium erythraea Rafn, as altered by ploidy level. in Industrial Crops and Products. 2022;186:115146.
doi:10.1016/j.indcrop.2022.115146 .

Filipović, Biljana, Skorić, Marijana, Gašić, Uroš, Dragićević, Milan, Božunović, Jelena, Matekalo, Dragana, Nestorović Živković, Jasmina, Banjanac, Tijana, Šiler, Branislav, Bohanec, Borut, Milutinović, Milica, Mišić, Danijela, "Spatial and temporal patterns of secoiridoid and xanthone biosynthetic pathways during early development of Centaurium erythraea Rafn, as altered by ploidy level" in Industrial Crops and Products, 186 (2022):115146,
https://doi.org/10.1016/j.indcrop.2022.115146 . .

TY  - JOUR
AU  - Banjanac, Tijana
AU  - Dragićević, Milan
AU  - Šiler, Branislav
AU  - Gašić, Uroš
AU  - Bohanec, Borut
AU  - Nestorović Živković, Jasmina
AU  - Trifunović, Snežana
AU  - Mišić, Danijela
PY  - 2017
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0031942217301541
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2742
AB  - Species within the genus Centaurium readily hybridize and polyploid complexes are often seen in natural populations. We describe phytochemical profiles of newly discovered allohexaploid hybrid, here named Centaurium pannonicum, and its parental tetraploid species C. erythraea and rare C. littorale ssp. compressum. Our aim was to examine chemodiversity of these taxa in the area of Vojvodina (North Serbia) and to perform metabolomics search for chemical classifiers which would provide high resolution discrimination of parental and hybrid individuals. In sum, UHPLC-MS/MS Orbitrap metabolomics fingerprinting revealed seventy compounds in methanol extracts. Despite the lack of qualitative chemical novelty in hybrid plants, UHPLC-qqqMS targeted metabolomics approach, aimed at three secoiridoid compounds and seventeen phenolics, pointed to considerable differences in quantitative composition of these dominant compounds among the plant taxa studied. In addition to the difference in the ploidy levels, the hybrid taxon was well distinguished from both parental species based on metabolite profiles, and, for most individuals, positioned intermediately to the parental taxa in both PCA and hierarchical clustering. After optimizing and comparing several statistical learning methods, it was possible to narrow the number of taxonomic classifiers to five (three xanthones, one secoiridoid glycoside, and one phenolic acid), while increasing the differentiation resolution. The presented metabolomics approach will certainly, along with morphometrics and molecular genetics studies, have high impact on further elucidation of complex relationships among taxa within the genus Centaurium.
T2  - Phytochemistry
T1  - Chemodiversity of two closely related tetraploid Centaurium species and their hexaploid hybrid: Metabolomic search for high-resolution taxonomic classifiers
VL  - 140
DO  - 10.1016/j.phytochem.2017.04.005
SP  - 27
EP  - 44
ER  - 

@article{
author = "Banjanac, Tijana and Dragićević, Milan and Šiler, Branislav and Gašić, Uroš and Bohanec, Borut and Nestorović Živković, Jasmina and Trifunović, Snežana and Mišić, Danijela",
year = "2017",
abstract = "Species within the genus Centaurium readily hybridize and polyploid complexes are often seen in natural populations. We describe phytochemical profiles of newly discovered allohexaploid hybrid, here named Centaurium pannonicum, and its parental tetraploid species C. erythraea and rare C. littorale ssp. compressum. Our aim was to examine chemodiversity of these taxa in the area of Vojvodina (North Serbia) and to perform metabolomics search for chemical classifiers which would provide high resolution discrimination of parental and hybrid individuals. In sum, UHPLC-MS/MS Orbitrap metabolomics fingerprinting revealed seventy compounds in methanol extracts. Despite the lack of qualitative chemical novelty in hybrid plants, UHPLC-qqqMS targeted metabolomics approach, aimed at three secoiridoid compounds and seventeen phenolics, pointed to considerable differences in quantitative composition of these dominant compounds among the plant taxa studied. In addition to the difference in the ploidy levels, the hybrid taxon was well distinguished from both parental species based on metabolite profiles, and, for most individuals, positioned intermediately to the parental taxa in both PCA and hierarchical clustering. After optimizing and comparing several statistical learning methods, it was possible to narrow the number of taxonomic classifiers to five (three xanthones, one secoiridoid glycoside, and one phenolic acid), while increasing the differentiation resolution. The presented metabolomics approach will certainly, along with morphometrics and molecular genetics studies, have high impact on further elucidation of complex relationships among taxa within the genus Centaurium.",
journal = "Phytochemistry",
title = "Chemodiversity of two closely related tetraploid Centaurium species and their hexaploid hybrid: Metabolomic search for high-resolution taxonomic classifiers",
volume = "140",
doi = "10.1016/j.phytochem.2017.04.005",
pages = "27-44"
}

Banjanac, T., Dragićević, M., Šiler, B., Gašić, U., Bohanec, B., Nestorović Živković, J., Trifunović, S.,& Mišić, D.. (2017). Chemodiversity of two closely related tetraploid Centaurium species and their hexaploid hybrid: Metabolomic search for high-resolution taxonomic classifiers. in Phytochemistry, 140, 27-44.
https://doi.org/10.1016/j.phytochem.2017.04.005

Banjanac T, Dragićević M, Šiler B, Gašić U, Bohanec B, Nestorović Živković J, Trifunović S, Mišić D. Chemodiversity of two closely related tetraploid Centaurium species and their hexaploid hybrid: Metabolomic search for high-resolution taxonomic classifiers. in Phytochemistry. 2017;140:27-44.
doi:10.1016/j.phytochem.2017.04.005 .

Banjanac, Tijana, Dragićević, Milan, Šiler, Branislav, Gašić, Uroš, Bohanec, Borut, Nestorović Živković, Jasmina, Trifunović, Snežana, Mišić, Danijela, "Chemodiversity of two closely related tetraploid Centaurium species and their hexaploid hybrid: Metabolomic search for high-resolution taxonomic classifiers" in Phytochemistry, 140 (2017):27-44,
https://doi.org/10.1016/j.phytochem.2017.04.005 . .

TY  - GEN
AU  - Vinterhalter, Branka
AU  - Zdravković-Korać, Snežana
AU  - Banjac, Nevena
AU  - Bohanec, Borut
AU  - Vinterhalter, Dragan
AU  - Savić, Jelena
PY  - 2016
UR  - http://link.springer.com/10.1007/s11738-016-2264-x
UR  - https://www.scopus.com/record/display.uri?eid=2-s2.0-84989910868&origin=SingleRecordEmailAlert&txGid=66B9A38F509F4540CF41E07DD21FB6C0.wsnAw8kcdt7IPYLO0V48gA:1
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2984
AB  - The corresponding author of this paper, Jelena Savić, has acknowledged that she manipulated two bands in Fig. 5c. The original figure is given here. The modification does not alter the interpretation of the data in any way. The author sincerely regrets the mistake and would like to apologize to her co-authors, the editors and readers (Figure presented).
PB  - Polish Academy of Sciences
T2  - Acta Physiologiae Plantarum
T1  - Erratum to: Effect of sucrose on shoot regeneration in Agrobacterium transformed Hypericum perforatum L. roots
IS  - 10
VL  - 38
DO  - 10.1007/s11738-016-2264-x
SP  - 253
ER  - 

@misc{
author = "Vinterhalter, Branka and Zdravković-Korać, Snežana and Banjac, Nevena and Bohanec, Borut and Vinterhalter, Dragan and Savić, Jelena",
year = "2016",
abstract = "The corresponding author of this paper, Jelena Savić, has acknowledged that she manipulated two bands in Fig. 5c. The original figure is given here. The modification does not alter the interpretation of the data in any way. The author sincerely regrets the mistake and would like to apologize to her co-authors, the editors and readers (Figure presented).",
publisher = "Polish Academy of Sciences",
journal = "Acta Physiologiae Plantarum",
title = "Erratum to: Effect of sucrose on shoot regeneration in Agrobacterium transformed Hypericum perforatum L. roots",
number = "10",
volume = "38",
doi = "10.1007/s11738-016-2264-x",
pages = "253"
}

Vinterhalter, B., Zdravković-Korać, S., Banjac, N., Bohanec, B., Vinterhalter, D.,& Savić, J.. (2016). Erratum to: Effect of sucrose on shoot regeneration in Agrobacterium transformed Hypericum perforatum L. roots. in Acta Physiologiae Plantarum
Polish Academy of Sciences., 38(10), 253.
https://doi.org/10.1007/s11738-016-2264-x

Vinterhalter B, Zdravković-Korać S, Banjac N, Bohanec B, Vinterhalter D, Savić J. Erratum to: Effect of sucrose on shoot regeneration in Agrobacterium transformed Hypericum perforatum L. roots. in Acta Physiologiae Plantarum. 2016;38(10):253.
doi:10.1007/s11738-016-2264-x .

Vinterhalter, Branka, Zdravković-Korać, Snežana, Banjac, Nevena, Bohanec, Borut, Vinterhalter, Dragan, Savić, Jelena, "Erratum to: Effect of sucrose on shoot regeneration in Agrobacterium transformed Hypericum perforatum L. roots" in Acta Physiologiae Plantarum, 38, no. 10 (2016):253,
https://doi.org/10.1007/s11738-016-2264-x . .

TY  - JOUR
AU  - Vinterhalter, Branka
AU  - Zdravković-Korać, Snežana
AU  - Banjac, Nevena
AU  - Bohanec, Borut
AU  - Vinterhalter, Dragan
AU  - Savić, Jelena
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2006
AB  - The aim of the present study was to investigate the effect of sucrose on
   shoot regeneration potential in Hypericum perforatum L. roots obtained
   by Agrobacterium rhizogenes transformation. The morphological evaluation
   of transgenic roots grown on media supplemented with sucrose (0.5, 1, 2,
   4, 6 and 8 \%) indicated that both genotype and sucrose concentration
   significantly affected root elongation and branching, as well as shoot
   regeneration. For two of five analyzed clones, lower sucrose
   concentrations (up to 2 \%) led to intensive shoot regeneration, while
   the other three clones intensified shoot development only at elevated
   sucrose concentrations (4 \%). For all clones, concentrations above 4 \%
   had a deleterious effect on both root and shoot development. Genetic
   characterization of regenerated shoots revealed that all tested clones
   were diploid with an average of 0.670 +/- 0.002 pg of DNA per nucleus,
   with no significant differences between transgenic and non-transformed
   plants and, according to PCR, with integrated A. rhizogenes rolA, -B, -C
   and -D genes. Real-time RT-PCR confirmed the expression of rolA, -B and
   -C, while expression of the rolD gene was not detected. Differences were
   detected in the absolute amounts of transcripts between analyzed clones,
   with the highest levels of expression for all three analyzed rol genes
   in a clone previously defined as having high root differentiation and
   less effective shoot regeneration potential. The observed variations in
   morphogenesis potential could be attributed to different levels of
   expression of integrated rolA, -B and -Cgenes; while sucrose
   additionally pointed out these trends.
T2  - Acta Physiologiae Plantarum
T1  - Effect of sucrose on shoot regeneration in Agrobacterium transformed
 Hypericum perforatum L. roots
VL  - 37
DO  - 10.1007/s11738-015-1785-z
SP  - 37
ER  - 

@article{
author = "Vinterhalter, Branka and Zdravković-Korać, Snežana and Banjac, Nevena and Bohanec, Borut and Vinterhalter, Dragan and Savić, Jelena",
year = "2015",
abstract = "The aim of the present study was to investigate the effect of sucrose on
   shoot regeneration potential in Hypericum perforatum L. roots obtained
   by Agrobacterium rhizogenes transformation. The morphological evaluation
   of transgenic roots grown on media supplemented with sucrose (0.5, 1, 2,
   4, 6 and 8 \%) indicated that both genotype and sucrose concentration
   significantly affected root elongation and branching, as well as shoot
   regeneration. For two of five analyzed clones, lower sucrose
   concentrations (up to 2 \%) led to intensive shoot regeneration, while
   the other three clones intensified shoot development only at elevated
   sucrose concentrations (4 \%). For all clones, concentrations above 4 \%
   had a deleterious effect on both root and shoot development. Genetic
   characterization of regenerated shoots revealed that all tested clones
   were diploid with an average of 0.670 +/- 0.002 pg of DNA per nucleus,
   with no significant differences between transgenic and non-transformed
   plants and, according to PCR, with integrated A. rhizogenes rolA, -B, -C
   and -D genes. Real-time RT-PCR confirmed the expression of rolA, -B and
   -C, while expression of the rolD gene was not detected. Differences were
   detected in the absolute amounts of transcripts between analyzed clones,
   with the highest levels of expression for all three analyzed rol genes
   in a clone previously defined as having high root differentiation and
   less effective shoot regeneration potential. The observed variations in
   morphogenesis potential could be attributed to different levels of
   expression of integrated rolA, -B and -Cgenes; while sucrose
   additionally pointed out these trends.",
journal = "Acta Physiologiae Plantarum",
title = "Effect of sucrose on shoot regeneration in Agrobacterium transformed
 Hypericum perforatum L. roots",
volume = "37",
doi = "10.1007/s11738-015-1785-z",
pages = "37"
}

Vinterhalter, B., Zdravković-Korać, S., Banjac, N., Bohanec, B., Vinterhalter, D.,& Savić, J.. (2015). Effect of sucrose on shoot regeneration in Agrobacterium transformed
 Hypericum perforatum L. roots. in Acta Physiologiae Plantarum, 37, 37.
https://doi.org/10.1007/s11738-015-1785-z

Vinterhalter B, Zdravković-Korać S, Banjac N, Bohanec B, Vinterhalter D, Savić J. Effect of sucrose on shoot regeneration in Agrobacterium transformed
 Hypericum perforatum L. roots. in Acta Physiologiae Plantarum. 2015;37:37.
doi:10.1007/s11738-015-1785-z .

Vinterhalter, Branka, Zdravković-Korać, Snežana, Banjac, Nevena, Bohanec, Borut, Vinterhalter, Dragan, Savić, Jelena, "Effect of sucrose on shoot regeneration in Agrobacterium transformed
 Hypericum perforatum L. roots" in Acta Physiologiae Plantarum, 37 (2015):37,
https://doi.org/10.1007/s11738-015-1785-z . .

TY  - JOUR
AU  - Ćosić, Tatjana
AU  - Motyka, Vaclav
AU  - Raspor, Martin
AU  - Savic, Jelena
AU  - Cingel, Aleksandar
AU  - Vinterhalter, Branka
AU  - Vinterhalter, Dragan
AU  - Travnickova, Alena
AU  - Dobrev, Petre I.
AU  - Bohanec, Borut
AU  - Ninković, Slavica
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1956
AB  - Kohlrabi (Brassica oleracea var. gongylodes) cultivars Vienna Purple
   (VP) and Vienna White (VW) were tested for their ability of de novo
   organogenesis in vitro. Root, cotyledon, hypocotyl explants and intact
   seedlings were cultivated on Murashige and Skoog (MS) media supplemented
   with different cytokinins: benzyladenine (BA), thidiazuron (TDZ), trans-
   or cis-zeatin. All tested cytokinins, including cis-zeatin, induced
   shoot regeneration from hypocotyl explants and intact seedlings, with
   seedlings being most successful for regeneration efficiency and
   viability of regenerated shoots in both cultivars. The highest frequency
   of shoot regeneration was achieved on MS with BA (60 \%) or TDZ (50 \%)
   for VP; and with BA (50 \%), TDZ (47.5 \%) or transZ (37.5 \%) for VW.
   Measurements of the endogenous cytokinin and indole-3-acetic acid (IAA)
   contents in both hypocotyl explants and seedlings with regenerated
   shoots (HRSs and SRSs) suggested that the observed differences in
   organogenic response between these two types of explants were related to
   their cytokinin and IAA contents. HRSs generally exhibited elevated
   amounts of total cytokinins, while SRSs displayed a higher IAA/bioactive
   cytokinins ratio. Shoots regenerated from seedlings were further
   successfully multiplicated on a medium supplemented with BA (0.5 mg
   L-1). The rooting potential of multiplicated shoots was tested on media
   supplemented with 2 or 4 mg L-1 indole-3-butyric acid (IBA), with the
   higher concentration of IBA leading to more efficient rooting. Rooted
   plantlets were successfully planted into soil and flow cytometric
   analysis did not reveal ploidy variations, indicating that the described
   protocol is fast and efficient for kohlrabi regeneration.
T2  - Plant Cell Tissue and Organ Culture
T1  - In vitro shoot organogenesis and comparative analysis of endogenous
 phytohormones in kohlrabi (Brassica oleracea var. gongylodes): effects
 of genotype, explant type and applied cytokinins
IS  - 3
VL  - 121
DO  - 10.1007/s11240-015-0742-2
SP  - 741
EP  - 760
ER  - 

@article{
author = "Ćosić, Tatjana and Motyka, Vaclav and Raspor, Martin and Savic, Jelena and Cingel, Aleksandar and Vinterhalter, Branka and Vinterhalter, Dragan and Travnickova, Alena and Dobrev, Petre I. and Bohanec, Borut and Ninković, Slavica",
year = "2015",
abstract = "Kohlrabi (Brassica oleracea var. gongylodes) cultivars Vienna Purple
   (VP) and Vienna White (VW) were tested for their ability of de novo
   organogenesis in vitro. Root, cotyledon, hypocotyl explants and intact
   seedlings were cultivated on Murashige and Skoog (MS) media supplemented
   with different cytokinins: benzyladenine (BA), thidiazuron (TDZ), trans-
   or cis-zeatin. All tested cytokinins, including cis-zeatin, induced
   shoot regeneration from hypocotyl explants and intact seedlings, with
   seedlings being most successful for regeneration efficiency and
   viability of regenerated shoots in both cultivars. The highest frequency
   of shoot regeneration was achieved on MS with BA (60 \%) or TDZ (50 \%)
   for VP; and with BA (50 \%), TDZ (47.5 \%) or transZ (37.5 \%) for VW.
   Measurements of the endogenous cytokinin and indole-3-acetic acid (IAA)
   contents in both hypocotyl explants and seedlings with regenerated
   shoots (HRSs and SRSs) suggested that the observed differences in
   organogenic response between these two types of explants were related to
   their cytokinin and IAA contents. HRSs generally exhibited elevated
   amounts of total cytokinins, while SRSs displayed a higher IAA/bioactive
   cytokinins ratio. Shoots regenerated from seedlings were further
   successfully multiplicated on a medium supplemented with BA (0.5 mg
   L-1). The rooting potential of multiplicated shoots was tested on media
   supplemented with 2 or 4 mg L-1 indole-3-butyric acid (IBA), with the
   higher concentration of IBA leading to more efficient rooting. Rooted
   plantlets were successfully planted into soil and flow cytometric
   analysis did not reveal ploidy variations, indicating that the described
   protocol is fast and efficient for kohlrabi regeneration.",
journal = "Plant Cell Tissue and Organ Culture",
title = "In vitro shoot organogenesis and comparative analysis of endogenous
 phytohormones in kohlrabi (Brassica oleracea var. gongylodes): effects
 of genotype, explant type and applied cytokinins",
number = "3",
volume = "121",
doi = "10.1007/s11240-015-0742-2",
pages = "741-760"
}

Ćosić, T., Motyka, V., Raspor, M., Savic, J., Cingel, A., Vinterhalter, B., Vinterhalter, D., Travnickova, A., Dobrev, P. I., Bohanec, B.,& Ninković, S.. (2015). In vitro shoot organogenesis and comparative analysis of endogenous
 phytohormones in kohlrabi (Brassica oleracea var. gongylodes): effects
 of genotype, explant type and applied cytokinins. in Plant Cell Tissue and Organ Culture, 121(3), 741-760.
https://doi.org/10.1007/s11240-015-0742-2

Ćosić T, Motyka V, Raspor M, Savic J, Cingel A, Vinterhalter B, Vinterhalter D, Travnickova A, Dobrev PI, Bohanec B, Ninković S. In vitro shoot organogenesis and comparative analysis of endogenous
 phytohormones in kohlrabi (Brassica oleracea var. gongylodes): effects
 of genotype, explant type and applied cytokinins. in Plant Cell Tissue and Organ Culture. 2015;121(3):741-760.
doi:10.1007/s11240-015-0742-2 .

Ćosić, Tatjana, Motyka, Vaclav, Raspor, Martin, Savic, Jelena, Cingel, Aleksandar, Vinterhalter, Branka, Vinterhalter, Dragan, Travnickova, Alena, Dobrev, Petre I., Bohanec, Borut, Ninković, Slavica, "In vitro shoot organogenesis and comparative analysis of endogenous
 phytohormones in kohlrabi (Brassica oleracea var. gongylodes): effects
 of genotype, explant type and applied cytokinins" in Plant Cell Tissue and Organ Culture, 121, no. 3 (2015):741-760,
https://doi.org/10.1007/s11240-015-0742-2 . .

TY  - JOUR
AU  - Nikolić, Radomirka R
AU  - Zdravković-Korać, Snežana
AU  - Ninković, Slavica
AU  - Dragićević, Milan
AU  - Miljuš-Đukić, Jovanka D.
AU  - Banović, Bojana
AU  - Bohanec, Borut
AU  - Savić, Jelena
AU  - Banjac, Nevena
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/957
AB  - Resistance to the non-selective herbicide dl-phosphinothricin (PPT) was introduced into commercial Lotus corniculatus cv. Bokor by co-cultivation of cotyledons with Agrobacterium tumefaciensAGL1 harbouring the binary vector pDM805 which contains the bialaphos resistance gene (bar) from Streptomyces hygroscopicus encoding phosphinothricin acetyltransferase (PAT) and the uidA gene encoding -glucuronidase. The half-cotyledon explants were precultured on regeneration Murashige and Skoog's (MS) medium supplemented with 6-benzyladenine (BA) and 1-naphthaleneacetic acid (NAA) at 0.5mgL(-1) each, 3days prior to infection. Upon co-cultivation, the explants were cultured on PPT-free regeneration medium for 10days, and then subcultured on regeneration/selection media with increasing PPT concentrations (5-7mgL(-1)) for about 18weeks. Out of 480 initially co-cultivated explants, 272 regenerated shoots survived the entire PPT selection procedure. Resistant shoots were grown further, multiplied by tillering that was additionally promoted by PPT and rooted on hormone-free MS medium containing 5mgL(-1) PPT. Established shoot cultures, continuously maintained on the same medium, have preserved PPT resistance up to now (more than 2years). Transformed plants assessed in vitro and in a greenhouse were tolerant to the herbicide PPT at 300mgL(-1) equivalent to more than twofold the recommended field dosage for weed eradication. Applied PPT treatment did not affect the activities of glutamine synthetase (GS; EC 6.3.1.2) and NADH-dependent glutamate dehydrogenase (NADH-GDH; EC 1.4.1.2) in transformed plants. However, PPT did increase the mobility of glutamine synthetase isoforms GS1 and GS2 as well as the inhibition of an additional high mobility GS (hmGS) activity. In untransformed plants, PPT treatment reduced total GS activity by 4.4-fold while contrary the activity of NADH-GDH was increased by ninefold. All transformed herbicide-resistant plants were phenotypically normal and exhibited genomic stability, as were the untransformed plants analysed by flow cytometry. Under greenhouse conditions, they grew to maturity, flowered and set seeds. Stable integration and expression of the bar gene in T0 and T1 plants were confirmed by Southern and Western blot analysis, while integration of the reporter uidA gene did not occur. The bar gene was inherited in a Mendelian fashion by the progeny, as detected by PPT resistance. The production of PPT-resistant plants may have significant practical applications in weed control in fields of L. corniculatus.
T2  - Annals of Applied Biology
T1  - Fertile transgenic Lotus corniculatus resistant to the non-selective herbicide phosphinothricin
IS  - 3
VL  - 163
DO  - 10.1111/aab.12071
SP  - 247
EP  - 493
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_957
ER  - 

@article{
author = "Nikolić, Radomirka R and Zdravković-Korać, Snežana and Ninković, Slavica and Dragićević, Milan and Miljuš-Đukić, Jovanka D. and Banović, Bojana and Bohanec, Borut and Savić, Jelena and Banjac, Nevena",
year = "2013",
abstract = "Resistance to the non-selective herbicide dl-phosphinothricin (PPT) was introduced into commercial Lotus corniculatus cv. Bokor by co-cultivation of cotyledons with Agrobacterium tumefaciensAGL1 harbouring the binary vector pDM805 which contains the bialaphos resistance gene (bar) from Streptomyces hygroscopicus encoding phosphinothricin acetyltransferase (PAT) and the uidA gene encoding -glucuronidase. The half-cotyledon explants were precultured on regeneration Murashige and Skoog's (MS) medium supplemented with 6-benzyladenine (BA) and 1-naphthaleneacetic acid (NAA) at 0.5mgL(-1) each, 3days prior to infection. Upon co-cultivation, the explants were cultured on PPT-free regeneration medium for 10days, and then subcultured on regeneration/selection media with increasing PPT concentrations (5-7mgL(-1)) for about 18weeks. Out of 480 initially co-cultivated explants, 272 regenerated shoots survived the entire PPT selection procedure. Resistant shoots were grown further, multiplied by tillering that was additionally promoted by PPT and rooted on hormone-free MS medium containing 5mgL(-1) PPT. Established shoot cultures, continuously maintained on the same medium, have preserved PPT resistance up to now (more than 2years). Transformed plants assessed in vitro and in a greenhouse were tolerant to the herbicide PPT at 300mgL(-1) equivalent to more than twofold the recommended field dosage for weed eradication. Applied PPT treatment did not affect the activities of glutamine synthetase (GS; EC 6.3.1.2) and NADH-dependent glutamate dehydrogenase (NADH-GDH; EC 1.4.1.2) in transformed plants. However, PPT did increase the mobility of glutamine synthetase isoforms GS1 and GS2 as well as the inhibition of an additional high mobility GS (hmGS) activity. In untransformed plants, PPT treatment reduced total GS activity by 4.4-fold while contrary the activity of NADH-GDH was increased by ninefold. All transformed herbicide-resistant plants were phenotypically normal and exhibited genomic stability, as were the untransformed plants analysed by flow cytometry. Under greenhouse conditions, they grew to maturity, flowered and set seeds. Stable integration and expression of the bar gene in T0 and T1 plants were confirmed by Southern and Western blot analysis, while integration of the reporter uidA gene did not occur. The bar gene was inherited in a Mendelian fashion by the progeny, as detected by PPT resistance. The production of PPT-resistant plants may have significant practical applications in weed control in fields of L. corniculatus.",
journal = "Annals of Applied Biology",
title = "Fertile transgenic Lotus corniculatus resistant to the non-selective herbicide phosphinothricin",
number = "3",
volume = "163",
doi = "10.1111/aab.12071",
pages = "247-493",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_957"
}

Nikolić, R. R., Zdravković-Korać, S., Ninković, S., Dragićević, M., Miljuš-Đukić, J. D., Banović, B., Bohanec, B., Savić, J.,& Banjac, N.. (2013). Fertile transgenic Lotus corniculatus resistant to the non-selective herbicide phosphinothricin. in Annals of Applied Biology, 163(3), 247-493.
https://doi.org/10.1111/aab.12071
https://hdl.handle.net/21.15107/rcub_ibiss_957

Nikolić RR, Zdravković-Korać S, Ninković S, Dragićević M, Miljuš-Đukić JD, Banović B, Bohanec B, Savić J, Banjac N. Fertile transgenic Lotus corniculatus resistant to the non-selective herbicide phosphinothricin. in Annals of Applied Biology. 2013;163(3):247-493.
doi:10.1111/aab.12071
https://hdl.handle.net/21.15107/rcub_ibiss_957 .

Nikolić, Radomirka R, Zdravković-Korać, Snežana, Ninković, Slavica, Dragićević, Milan, Miljuš-Đukić, Jovanka D., Banović, Bojana, Bohanec, Borut, Savić, Jelena, Banjac, Nevena, "Fertile transgenic Lotus corniculatus resistant to the non-selective herbicide phosphinothricin" in Annals of Applied Biology, 163, no. 3 (2013):247-493,
https://doi.org/10.1111/aab.12071 .,
https://hdl.handle.net/21.15107/rcub_ibiss_957 .

TY  - JOUR
AU  - Ćalić, Dušica
AU  - Bohanec, Borut
AU  - Devrnja, Nina
AU  - Milojević, Jelena
AU  - Tubić, Ljiljana B
AU  - Kostić, Igor
AU  - Zdravković-Korać, Snežana
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1006
AB  - Horse chestnut (Aesculus hyppocastanum L., Hyppocastanacea) is a relict species with a slow and complex reproductive cycle considered to have horticultural and medical importance. The cycle maybe circumvented via in vitro androgenesis. Androgenesis of horse chestnut was induced in microspores and anther culture on MS media. Some of the horse chestnut androgenic embryos were albinos. Addition of abscisic acid in media (in concentrations of 0.01, 0.1, 0.5, 1, 2, 5, 10, and 20 mg l(-1)) with horse chestnut androgenic embryos has circumvented the reproduction cycle barriers. The best results were achieved on medium with the lowest abscisic acid concentration (0.01 mg l(-1)) in microspore culture. The microspore culture proved to be a better model system for embryo production and albino embryo reduction than anther culture. Flow cytometry analysis after maturation treatments induced by ABA showed that 88 % of green embryos originating from microspore culture were haploid. However, 50 % of green embryos from anther culture were haploid. The remaining analyzed androgenic embryos, from both types of cultures were diploid.
T2  - Trees-Structure and Function
T1  - Impact of abscisic acid in overcoming the problem of albinism in horse chestnut androgenic embryos
IS  - 3
VL  - 27
SP  - 99
EP  - 762
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1006
ER  - 

@article{
author = "Ćalić, Dušica and Bohanec, Borut and Devrnja, Nina and Milojević, Jelena and Tubić, Ljiljana B and Kostić, Igor and Zdravković-Korać, Snežana",
year = "2013",
abstract = "Horse chestnut (Aesculus hyppocastanum L., Hyppocastanacea) is a relict species with a slow and complex reproductive cycle considered to have horticultural and medical importance. The cycle maybe circumvented via in vitro androgenesis. Androgenesis of horse chestnut was induced in microspores and anther culture on MS media. Some of the horse chestnut androgenic embryos were albinos. Addition of abscisic acid in media (in concentrations of 0.01, 0.1, 0.5, 1, 2, 5, 10, and 20 mg l(-1)) with horse chestnut androgenic embryos has circumvented the reproduction cycle barriers. The best results were achieved on medium with the lowest abscisic acid concentration (0.01 mg l(-1)) in microspore culture. The microspore culture proved to be a better model system for embryo production and albino embryo reduction than anther culture. Flow cytometry analysis after maturation treatments induced by ABA showed that 88 % of green embryos originating from microspore culture were haploid. However, 50 % of green embryos from anther culture were haploid. The remaining analyzed androgenic embryos, from both types of cultures were diploid.",
journal = "Trees-Structure and Function",
title = "Impact of abscisic acid in overcoming the problem of albinism in horse chestnut androgenic embryos",
number = "3",
volume = "27",
pages = "99-762",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1006"
}

Ćalić, D., Bohanec, B., Devrnja, N., Milojević, J., Tubić, L. B., Kostić, I.,& Zdravković-Korać, S.. (2013). Impact of abscisic acid in overcoming the problem of albinism in horse chestnut androgenic embryos. in Trees-Structure and Function, 27(3), 99-762.
https://hdl.handle.net/21.15107/rcub_ibiss_1006

Ćalić D, Bohanec B, Devrnja N, Milojević J, Tubić LB, Kostić I, Zdravković-Korać S. Impact of abscisic acid in overcoming the problem of albinism in horse chestnut androgenic embryos. in Trees-Structure and Function. 2013;27(3):99-762.
https://hdl.handle.net/21.15107/rcub_ibiss_1006 .

Ćalić, Dušica, Bohanec, Borut, Devrnja, Nina, Milojević, Jelena, Tubić, Ljiljana B, Kostić, Igor, Zdravković-Korać, Snežana, "Impact of abscisic acid in overcoming the problem of albinism in horse chestnut androgenic embryos" in Trees-Structure and Function, 27, no. 3 (2013):99-762,
https://hdl.handle.net/21.15107/rcub_ibiss_1006 .

TY  - JOUR
AU  - Ćosić, Tatjana
AU  - Vinterhalter, Branka
AU  - Vinterhalter, Dragan V
AU  - Banjac, Nevena
AU  - Cingel, Aleksandar
AU  - Savić, Jelena M.
AU  - Bohanec, Borut
AU  - Ninković, Slavica
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1007
AB  - A simple and efficient protocol for direct somatic embryogenesis and plant regeneration of kohlrabi (Brassica oleracea var. gongylodes) was developed. Somatic embryos were induced from immature zygotic embryos at different developmental stages cultured on Murashige and Skoog medium supplemented with 0, 0.5, 1.0, or 1.5 mg/l 2,4-dichlorophenoxyacetic acid. Zygotic embryos at the early cotyledonary stage, which were cultured for 4 wk on plant growth regulator-free (PGR-free) medium, displayed the highest percentage of somatic embryogenesis (80.7%). Embryogenic tissue could be subcultured on the same medium for over 1 yr. Embryogenic lines derived from early cotyledonary stage zygotic embryos displayed the highest intensity of secondary embryogenesis (highest mean number of new somatic embryos per responsive somatic embryo explant). Histological analyses confirmed the direct origin of the secondary somatic embryos. Prolonged culturing of embryogenic tissue on PGR-free medium led to somatic embryo development into plantlets that were successfully acclimated in the greenhouse with a survival rate of 72.5%. Flow cytometry analysis showed no ploidy variation in 96.7% of the acclimated plants.
T2  - In Vitro Cellular & Developmental Biology-Plant
T1  - In vitro plant regeneration from immature zygotic embryos and repetitive somatic embryogenesis in kohlrabi (Brassica oleracea var. gongylodes)
IS  - 3
VL  - 49
SP  - 294
EP  - 303
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1007
ER  - 

@article{
author = "Ćosić, Tatjana and Vinterhalter, Branka and Vinterhalter, Dragan V and Banjac, Nevena and Cingel, Aleksandar and Savić, Jelena M. and Bohanec, Borut and Ninković, Slavica",
year = "2013",
abstract = "A simple and efficient protocol for direct somatic embryogenesis and plant regeneration of kohlrabi (Brassica oleracea var. gongylodes) was developed. Somatic embryos were induced from immature zygotic embryos at different developmental stages cultured on Murashige and Skoog medium supplemented with 0, 0.5, 1.0, or 1.5 mg/l 2,4-dichlorophenoxyacetic acid. Zygotic embryos at the early cotyledonary stage, which were cultured for 4 wk on plant growth regulator-free (PGR-free) medium, displayed the highest percentage of somatic embryogenesis (80.7%). Embryogenic tissue could be subcultured on the same medium for over 1 yr. Embryogenic lines derived from early cotyledonary stage zygotic embryos displayed the highest intensity of secondary embryogenesis (highest mean number of new somatic embryos per responsive somatic embryo explant). Histological analyses confirmed the direct origin of the secondary somatic embryos. Prolonged culturing of embryogenic tissue on PGR-free medium led to somatic embryo development into plantlets that were successfully acclimated in the greenhouse with a survival rate of 72.5%. Flow cytometry analysis showed no ploidy variation in 96.7% of the acclimated plants.",
journal = "In Vitro Cellular & Developmental Biology-Plant",
title = "In vitro plant regeneration from immature zygotic embryos and repetitive somatic embryogenesis in kohlrabi (Brassica oleracea var. gongylodes)",
number = "3",
volume = "49",
pages = "294-303",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1007"
}

Ćosić, T., Vinterhalter, B., Vinterhalter, D. V., Banjac, N., Cingel, A., Savić, J. M., Bohanec, B.,& Ninković, S.. (2013). In vitro plant regeneration from immature zygotic embryos and repetitive somatic embryogenesis in kohlrabi (Brassica oleracea var. gongylodes). in In Vitro Cellular & Developmental Biology-Plant, 49(3), 294-303.
https://hdl.handle.net/21.15107/rcub_ibiss_1007

Ćosić T, Vinterhalter B, Vinterhalter DV, Banjac N, Cingel A, Savić JM, Bohanec B, Ninković S. In vitro plant regeneration from immature zygotic embryos and repetitive somatic embryogenesis in kohlrabi (Brassica oleracea var. gongylodes). in In Vitro Cellular & Developmental Biology-Plant. 2013;49(3):294-303.
https://hdl.handle.net/21.15107/rcub_ibiss_1007 .

Ćosić, Tatjana, Vinterhalter, Branka, Vinterhalter, Dragan V, Banjac, Nevena, Cingel, Aleksandar, Savić, Jelena M., Bohanec, Borut, Ninković, Slavica, "In vitro plant regeneration from immature zygotic embryos and repetitive somatic embryogenesis in kohlrabi (Brassica oleracea var. gongylodes)" in In Vitro Cellular & Developmental Biology-Plant, 49, no. 3 (2013):294-303,
https://hdl.handle.net/21.15107/rcub_ibiss_1007 .