TY  - JOUR
AU  - Subotić, Srđan
AU  - Višnjić-Jeftić, Željka
AU  - Bojović, Srđan
AU  - Đikanović, Vesna
AU  - Krpo-Ćetković, Jasmina
AU  - Lenhardt, Mirjana
PY  - 2021
UR  - https://link.springer.com/10.1007/s11356-021-15073-0
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4438
AB  - Vimba bream (Vimba vimba) individuals were collected from the Danube River near Belgrade, from May 2016 to May 2017. Samples of muscle and liver tissue were analyzed for concentrations of 25 elements (macro-, micro-, and toxic elements) using inductively coupled plasma optical emission spectrometry (ICP-OES). For both tissues, the highest concentrations of most elements were observed in late spring/early summer (months of May and June). Majority of statistically significant correlations between elements were positive in both tissues. Contributions of Ca, K, and Mg to human diet were in a similar range. The lowest contribution of all macroelements was observed for Na, while the contribution of Se was well above the recommended dietary allowance for this element. Levels of detected elements were well below MAC values prescribed by national and international regulations. The highest value of Hg liver/muscle index (>1) was observed in August, which could indicate a higher contamination of the sampling location in that month. Se:Hg molar ratio never drops below 1, which could indicate that Hg toxicity is prevented. THQ values showed little variation thought the year and were well below the threshold of 1, with the exception of THQ for Al in May (0.92). This element also strongly affects the hazard index.
PB  - Springer Nature
T2  - Environmental Science and Pollution Research
T1  - Seasonal variations of macro-, micro-, and toxic elements in tissues of vimba bream (Vimba vimba) from the Danube River near Belgrade, Serbia.
DO  - 10.1007/s11356-021-15073-0
ER  - 

@article{
author = "Subotić, Srđan and Višnjić-Jeftić, Željka and Bojović, Srđan and Đikanović, Vesna and Krpo-Ćetković, Jasmina and Lenhardt, Mirjana",
year = "2021",
abstract = "Vimba bream (Vimba vimba) individuals were collected from the Danube River near Belgrade, from May 2016 to May 2017. Samples of muscle and liver tissue were analyzed for concentrations of 25 elements (macro-, micro-, and toxic elements) using inductively coupled plasma optical emission spectrometry (ICP-OES). For both tissues, the highest concentrations of most elements were observed in late spring/early summer (months of May and June). Majority of statistically significant correlations between elements were positive in both tissues. Contributions of Ca, K, and Mg to human diet were in a similar range. The lowest contribution of all macroelements was observed for Na, while the contribution of Se was well above the recommended dietary allowance for this element. Levels of detected elements were well below MAC values prescribed by national and international regulations. The highest value of Hg liver/muscle index (>1) was observed in August, which could indicate a higher contamination of the sampling location in that month. Se:Hg molar ratio never drops below 1, which could indicate that Hg toxicity is prevented. THQ values showed little variation thought the year and were well below the threshold of 1, with the exception of THQ for Al in May (0.92). This element also strongly affects the hazard index.",
publisher = "Springer Nature",
journal = "Environmental Science and Pollution Research",
title = "Seasonal variations of macro-, micro-, and toxic elements in tissues of vimba bream (Vimba vimba) from the Danube River near Belgrade, Serbia.",
doi = "10.1007/s11356-021-15073-0"
}

Subotić, S., Višnjić-Jeftić, Ž., Bojović, S., Đikanović, V., Krpo-Ćetković, J.,& Lenhardt, M.. (2021). Seasonal variations of macro-, micro-, and toxic elements in tissues of vimba bream (Vimba vimba) from the Danube River near Belgrade, Serbia.. in Environmental Science and Pollution Research
Springer Nature..
https://doi.org/10.1007/s11356-021-15073-0

Subotić S, Višnjić-Jeftić Ž, Bojović S, Đikanović V, Krpo-Ćetković J, Lenhardt M. Seasonal variations of macro-, micro-, and toxic elements in tissues of vimba bream (Vimba vimba) from the Danube River near Belgrade, Serbia.. in Environmental Science and Pollution Research. 2021;.
doi:10.1007/s11356-021-15073-0 .

Subotić, Srđan, Višnjić-Jeftić, Željka, Bojović, Srđan, Đikanović, Vesna, Krpo-Ćetković, Jasmina, Lenhardt, Mirjana, "Seasonal variations of macro-, micro-, and toxic elements in tissues of vimba bream (Vimba vimba) from the Danube River near Belgrade, Serbia." in Environmental Science and Pollution Research (2021),
https://doi.org/10.1007/s11356-021-15073-0 . .

TY  - JOUR
AU  - Subotić, Srđan
AU  - Višnjić-Jeftić, Željka
AU  - Lenhardt, Mirjana
AU  - Krpo-Ćetković, Jasmine
PY  - 2021
UR  - http://acta-zoologica-bulgarica.eu/2021/002427.pdf
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4449
AB  - Vimba bream individuals (n = 157), obtained from commercial catches in 2016 and 2017 near Belgrade. were analysed to obtain information on growth and condition of this species. The total body length of sampled fish ranged from 248 to 440 mm, while the body weight ranged from 139 to 889 g. Five age classes (from 4+ to 8+) were present in the sample, with predominance of individuals aged 6+. Back-calculated annual length growth was the highest in the first year of life. The total body length defined according to the parameters of the von Bertalanffy growth curve could be calculated at any given time t using 75.07 x (1-e(-0.06x(t+1.73))), and the length-weight relationship - using LogW = 0.010 + 3.02 x logTL. The asymptotic standard body length was SL infinity = 63.86 cm and the phi-prime growth performance index was phi'= 2.53. The highest mean values of Fulton's condition factor were observed in spring of 2016, followed by spring of 2017, and in individuals aged 7+. The seasonal and age-related differences in condition were statistically significant. The lack of data, the degree of endangerment in some European countries, the status of important species for the river continuity restoration and commercial exploitation of this species, emphasise the need for future research.
PB  - Sofia: Institute of Biodiversity and Ecosystem Research – Bulgarian Academy of Sciences
T2  - Acta Zoologica Bulgarica
T1  - Growth and Condition of Vimba Bream Vimba vimba ( L., 1758) (Actinopterygii: Cyprinidae) from Commercial Fisheries in the Danube River near Belgrade, Serbia
IS  - 2
VL  - 73
SP  - 261
EP  - 267
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4449
ER  - 

@article{
author = "Subotić, Srđan and Višnjić-Jeftić, Željka and Lenhardt, Mirjana and Krpo-Ćetković, Jasmine",
year = "2021",
abstract = "Vimba bream individuals (n = 157), obtained from commercial catches in 2016 and 2017 near Belgrade. were analysed to obtain information on growth and condition of this species. The total body length of sampled fish ranged from 248 to 440 mm, while the body weight ranged from 139 to 889 g. Five age classes (from 4+ to 8+) were present in the sample, with predominance of individuals aged 6+. Back-calculated annual length growth was the highest in the first year of life. The total body length defined according to the parameters of the von Bertalanffy growth curve could be calculated at any given time t using 75.07 x (1-e(-0.06x(t+1.73))), and the length-weight relationship - using LogW = 0.010 + 3.02 x logTL. The asymptotic standard body length was SL infinity = 63.86 cm and the phi-prime growth performance index was phi'= 2.53. The highest mean values of Fulton's condition factor were observed in spring of 2016, followed by spring of 2017, and in individuals aged 7+. The seasonal and age-related differences in condition were statistically significant. The lack of data, the degree of endangerment in some European countries, the status of important species for the river continuity restoration and commercial exploitation of this species, emphasise the need for future research.",
publisher = "Sofia: Institute of Biodiversity and Ecosystem Research – Bulgarian Academy of Sciences",
journal = "Acta Zoologica Bulgarica",
title = "Growth and Condition of Vimba Bream Vimba vimba ( L., 1758) (Actinopterygii: Cyprinidae) from Commercial Fisheries in the Danube River near Belgrade, Serbia",
number = "2",
volume = "73",
pages = "261-267",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4449"
}

Subotić, S., Višnjić-Jeftić, Ž., Lenhardt, M.,& Krpo-Ćetković, J.. (2021). Growth and Condition of Vimba Bream Vimba vimba ( L., 1758) (Actinopterygii: Cyprinidae) from Commercial Fisheries in the Danube River near Belgrade, Serbia. in Acta Zoologica Bulgarica
Sofia: Institute of Biodiversity and Ecosystem Research – Bulgarian Academy of Sciences., 73(2), 261-267.
https://hdl.handle.net/21.15107/rcub_ibiss_4449

Subotić S, Višnjić-Jeftić Ž, Lenhardt M, Krpo-Ćetković J. Growth and Condition of Vimba Bream Vimba vimba ( L., 1758) (Actinopterygii: Cyprinidae) from Commercial Fisheries in the Danube River near Belgrade, Serbia. in Acta Zoologica Bulgarica. 2021;73(2):261-267.
https://hdl.handle.net/21.15107/rcub_ibiss_4449 .

Subotić, Srđan, Višnjić-Jeftić, Željka, Lenhardt, Mirjana, Krpo-Ćetković, Jasmine, "Growth and Condition of Vimba Bream Vimba vimba ( L., 1758) (Actinopterygii: Cyprinidae) from Commercial Fisheries in the Danube River near Belgrade, Serbia" in Acta Zoologica Bulgarica, 73, no. 2 (2021):261-267,
https://hdl.handle.net/21.15107/rcub_ibiss_4449 .

TY  - CONF
AU  - Đorđević, Jelena
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Kolarević, Stoimir
AU  - Višnjić-Jeftić, Željka
AU  - Subotić, Srđan
AU  - Gačić, Zoran
AU  - Lenhardt, Mirjana
AU  - Vuković-Gačić, Branka
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4729
AB  - Water quality in the Danube River Basin is largely influenced by the inputs of pollutants - particularly
excessive nutrients, organic material, and hazardous substances, which affects the whole ecosystem
at various levels. The major goal of this study was to evaluate the level of genotoxic potential at the
site at Danube River, Novi Banovci, which is under significant anthropogenic pressure of untreated
municipal and industrial waste waters.
DNA damage in situ was assessed in 5 specimens of white bream (Blicca bjoerkna) erythrocytes, liver
and gills cells with comet assay and in erythrocytes with micronucleus test. Additionally, genotoxicity
of native water samples collected from the site and upstream and downstream of the site was
evaluated by the comet assay on HepG2 cells. Microscopic images of comets were scored using Comet
IV Computer Software (Perceptive Instruments, UK) and tail intensity was chosen as parameters to
assess the DNA damage.
Comet assay on white bream showed that gills were the most affected tissue, while liver showed the
smallest values. Micronuclei frequency was low in general, with mean of 1 micronuclei in 5000 scored
erythrocytes, with no significant correlation to the values of comet assay in erythrocytes. Comet assay
on HepG2 cells showed significant difference between values of negative control samples and samples
of water from effluent site and downstream of wastewater discharge. There was no significant
difference between negative control and upstream samples of water.
Our results showed that great impact of municipal and industrial wastewaters on the river ecosystem.
The application of in vitro and in situ tests gives a better insight into genotoxic effects. More
importantly, because Serbia still doesn’t have waste water treatme nt plants, this kind of research
points out the imperative for implementation of this facilities.
PB  - Neuburg:  Aueninstitut Neuburg
C3  - 43rd IAD Conference: Rivers and Floodplains in the Antropocene: Upcoming Challenges in the Danube River Basin
T1  - Genotoxicity assessment of Danube River: in situ and in vitro methods
SP  - 18
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4729
ER  - 

@conference{
author = "Đorđević, Jelena and Kostić-Vuković, Jovana and Sunjog, Karolina and Kolarević, Stoimir and Višnjić-Jeftić, Željka and Subotić, Srđan and Gačić, Zoran and Lenhardt, Mirjana and Vuković-Gačić, Branka",
year = "2021",
abstract = "Water quality in the Danube River Basin is largely influenced by the inputs of pollutants - particularly
excessive nutrients, organic material, and hazardous substances, which affects the whole ecosystem
at various levels. The major goal of this study was to evaluate the level of genotoxic potential at the
site at Danube River, Novi Banovci, which is under significant anthropogenic pressure of untreated
municipal and industrial waste waters.
DNA damage in situ was assessed in 5 specimens of white bream (Blicca bjoerkna) erythrocytes, liver
and gills cells with comet assay and in erythrocytes with micronucleus test. Additionally, genotoxicity
of native water samples collected from the site and upstream and downstream of the site was
evaluated by the comet assay on HepG2 cells. Microscopic images of comets were scored using Comet
IV Computer Software (Perceptive Instruments, UK) and tail intensity was chosen as parameters to
assess the DNA damage.
Comet assay on white bream showed that gills were the most affected tissue, while liver showed the
smallest values. Micronuclei frequency was low in general, with mean of 1 micronuclei in 5000 scored
erythrocytes, with no significant correlation to the values of comet assay in erythrocytes. Comet assay
on HepG2 cells showed significant difference between values of negative control samples and samples
of water from effluent site and downstream of wastewater discharge. There was no significant
difference between negative control and upstream samples of water.
Our results showed that great impact of municipal and industrial wastewaters on the river ecosystem.
The application of in vitro and in situ tests gives a better insight into genotoxic effects. More
importantly, because Serbia still doesn’t have waste water treatme nt plants, this kind of research
points out the imperative for implementation of this facilities.",
publisher = "Neuburg:  Aueninstitut Neuburg",
journal = "43rd IAD Conference: Rivers and Floodplains in the Antropocene: Upcoming Challenges in the Danube River Basin",
title = "Genotoxicity assessment of Danube River: in situ and in vitro methods",
pages = "18",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4729"
}

Đorđević, J., Kostić-Vuković, J., Sunjog, K., Kolarević, S., Višnjić-Jeftić, Ž., Subotić, S., Gačić, Z., Lenhardt, M.,& Vuković-Gačić, B.. (2021). Genotoxicity assessment of Danube River: in situ and in vitro methods. in 43rd IAD Conference: Rivers and Floodplains in the Antropocene: Upcoming Challenges in the Danube River Basin
Neuburg:  Aueninstitut Neuburg., 18.
https://hdl.handle.net/21.15107/rcub_ibiss_4729

Đorđević J, Kostić-Vuković J, Sunjog K, Kolarević S, Višnjić-Jeftić Ž, Subotić S, Gačić Z, Lenhardt M, Vuković-Gačić B. Genotoxicity assessment of Danube River: in situ and in vitro methods. in 43rd IAD Conference: Rivers and Floodplains in the Antropocene: Upcoming Challenges in the Danube River Basin. 2021;:18.
https://hdl.handle.net/21.15107/rcub_ibiss_4729 .

Đorđević, Jelena, Kostić-Vuković, Jovana, Sunjog, Karolina, Kolarević, Stoimir, Višnjić-Jeftić, Željka, Subotić, Srđan, Gačić, Zoran, Lenhardt, Mirjana, Vuković-Gačić, Branka, "Genotoxicity assessment of Danube River: in situ and in vitro methods" in 43rd IAD Conference: Rivers and Floodplains in the Antropocene: Upcoming Challenges in the Danube River Basin (2021):18,
https://hdl.handle.net/21.15107/rcub_ibiss_4729 .

TY  - JOUR
AU  - Spasić, Slađana
AU  - Subotić, Srđan
AU  - Višnjić-Jeftić, Željka
AU  - Lenhardt, Mirjana
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3646
AB  - Fish erythrocytes released from erythropoietic sites are immature. During circulation, they increase their own surface, while the cell body and nuclei begin to become more elliptical and less spherical during maturation. The relative abundance of different developmental stages represents an erythron profile which could be a more sensitive indicator of contamination than classic hematological indices. In this study, we analyzed blood smears of the common nase (Chondrostoma nasus) and vimba bream (Vimba vimba) with the main goal to identify the determinants of the developmental stage of erythrocytes. Based on parameters developmental stages, erythrocytes are categorized into immature, intermediate or mature. In this investigation we have used four classification methods: the Two Step Cluster analysis, the K-Means Cluster analysis, and Neural Networks–Multilayer Perceptron and an Ordinal Regression model. Our findings clearly justify that Multilayers Perceptron and OLR models are appropriate to classify the developmental stage of fish erythrocytes in both species.
T2  - Pattern Recognition and Image Analysis
T1  - Application of Different Classification Methods to Determine the Developmental Stage of Fish Erythrocytes of the Common Nase (Chondrostoma nasus) and Vimba Bream (Vimba vimba)
IS  - 1
VL  - 30
DO  - 10.1134/s1054661820010150
SP  - 43
EP  - 51
ER  - 

@article{
author = "Spasić, Slađana and Subotić, Srđan and Višnjić-Jeftić, Željka and Lenhardt, Mirjana",
year = "2020",
abstract = "Fish erythrocytes released from erythropoietic sites are immature. During circulation, they increase their own surface, while the cell body and nuclei begin to become more elliptical and less spherical during maturation. The relative abundance of different developmental stages represents an erythron profile which could be a more sensitive indicator of contamination than classic hematological indices. In this study, we analyzed blood smears of the common nase (Chondrostoma nasus) and vimba bream (Vimba vimba) with the main goal to identify the determinants of the developmental stage of erythrocytes. Based on parameters developmental stages, erythrocytes are categorized into immature, intermediate or mature. In this investigation we have used four classification methods: the Two Step Cluster analysis, the K-Means Cluster analysis, and Neural Networks–Multilayer Perceptron and an Ordinal Regression model. Our findings clearly justify that Multilayers Perceptron and OLR models are appropriate to classify the developmental stage of fish erythrocytes in both species.",
journal = "Pattern Recognition and Image Analysis",
title = "Application of Different Classification Methods to Determine the Developmental Stage of Fish Erythrocytes of the Common Nase (Chondrostoma nasus) and Vimba Bream (Vimba vimba)",
number = "1",
volume = "30",
doi = "10.1134/s1054661820010150",
pages = "43-51"
}

Spasić, S., Subotić, S., Višnjić-Jeftić, Ž.,& Lenhardt, M.. (2020). Application of Different Classification Methods to Determine the Developmental Stage of Fish Erythrocytes of the Common Nase (Chondrostoma nasus) and Vimba Bream (Vimba vimba). in Pattern Recognition and Image Analysis, 30(1), 43-51.
https://doi.org/10.1134/s1054661820010150

Spasić S, Subotić S, Višnjić-Jeftić Ž, Lenhardt M. Application of Different Classification Methods to Determine the Developmental Stage of Fish Erythrocytes of the Common Nase (Chondrostoma nasus) and Vimba Bream (Vimba vimba). in Pattern Recognition and Image Analysis. 2020;30(1):43-51.
doi:10.1134/s1054661820010150 .

Spasić, Slađana, Subotić, Srđan, Višnjić-Jeftić, Željka, Lenhardt, Mirjana, "Application of Different Classification Methods to Determine the Developmental Stage of Fish Erythrocytes of the Common Nase (Chondrostoma nasus) and Vimba Bream (Vimba vimba)" in Pattern Recognition and Image Analysis, 30, no. 1 (2020):43-51,
https://doi.org/10.1134/s1054661820010150 . .

TY  - JOUR
AU  - Subotić, Srđan
AU  - Višnjić-Jeftić, Željka
AU  - Đikanović, Vesna
AU  - Spasić, Slađana
AU  - Krpo-Ćetković, Jasmina
AU  - Lenhardt, Mirjana
PY  - 2019
UR  - http://link.springer.com/10.1007/s00128-019-02657-3
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3413
AB  - Accumulation of 17 elements in muscle and liver of common nase and vimba bream, caught between February and May 2016 in the Danube River (1173 river kilometer), were assessed by ICP-OES. The principal component analysis grouped muscle and liver samples based on element concentrations (muscle grouped by higher Ba and Sr values, and liver grouped by higher Al, B, Cd, Cr, Cu, Fe, Mn, and Zn values), but no grouping between the two species was observed. Concentrations of Ba, Cu, Fe, and Zn were significantly higher in muscle, and concentrations of Ba, Cd, Cu, and Mn in liver of common nase, while vimba bream had significantly higher concentrations of Cr and Fe in liver. Common nase has a higher affinity for bioaccumulation of Cu, Fe, and Zn in muscle, while vimba bream has a higher affinity for Al, Cd, and Cr in muscle.
T2  - Bulletin of Environmental Contamination and Toxicology
T1  - Metal Accumulation in Muscle and Liver of the Common Nase (Chondrostoma nasus) and Vimba Bream (Vimbavimba) from the Danube River, Serbia: Bioindicative Aspects.
VL  - 103
DO  - 10.1007/s00128-019-02657-3
SP  - 261
EP  - 266
ER  - 

@article{
author = "Subotić, Srđan and Višnjić-Jeftić, Željka and Đikanović, Vesna and Spasić, Slađana and Krpo-Ćetković, Jasmina and Lenhardt, Mirjana",
year = "2019",
abstract = "Accumulation of 17 elements in muscle and liver of common nase and vimba bream, caught between February and May 2016 in the Danube River (1173 river kilometer), were assessed by ICP-OES. The principal component analysis grouped muscle and liver samples based on element concentrations (muscle grouped by higher Ba and Sr values, and liver grouped by higher Al, B, Cd, Cr, Cu, Fe, Mn, and Zn values), but no grouping between the two species was observed. Concentrations of Ba, Cu, Fe, and Zn were significantly higher in muscle, and concentrations of Ba, Cd, Cu, and Mn in liver of common nase, while vimba bream had significantly higher concentrations of Cr and Fe in liver. Common nase has a higher affinity for bioaccumulation of Cu, Fe, and Zn in muscle, while vimba bream has a higher affinity for Al, Cd, and Cr in muscle.",
journal = "Bulletin of Environmental Contamination and Toxicology",
title = "Metal Accumulation in Muscle and Liver of the Common Nase (Chondrostoma nasus) and Vimba Bream (Vimbavimba) from the Danube River, Serbia: Bioindicative Aspects.",
volume = "103",
doi = "10.1007/s00128-019-02657-3",
pages = "261-266"
}

Subotić, S., Višnjić-Jeftić, Ž., Đikanović, V., Spasić, S., Krpo-Ćetković, J.,& Lenhardt, M.. (2019). Metal Accumulation in Muscle and Liver of the Common Nase (Chondrostoma nasus) and Vimba Bream (Vimbavimba) from the Danube River, Serbia: Bioindicative Aspects.. in Bulletin of Environmental Contamination and Toxicology, 103, 261-266.
https://doi.org/10.1007/s00128-019-02657-3

Subotić S, Višnjić-Jeftić Ž, Đikanović V, Spasić S, Krpo-Ćetković J, Lenhardt M. Metal Accumulation in Muscle and Liver of the Common Nase (Chondrostoma nasus) and Vimba Bream (Vimbavimba) from the Danube River, Serbia: Bioindicative Aspects.. in Bulletin of Environmental Contamination and Toxicology. 2019;103:261-266.
doi:10.1007/s00128-019-02657-3 .

Subotić, Srđan, Višnjić-Jeftić, Željka, Đikanović, Vesna, Spasić, Slađana, Krpo-Ćetković, Jasmina, Lenhardt, Mirjana, "Metal Accumulation in Muscle and Liver of the Common Nase (Chondrostoma nasus) and Vimba Bream (Vimbavimba) from the Danube River, Serbia: Bioindicative Aspects." in Bulletin of Environmental Contamination and Toxicology, 103 (2019):261-266,
https://doi.org/10.1007/s00128-019-02657-3 . .