TY  - CONF
AU  - Marković, Tijana
AU  - Aničić, Neda
AU  - Marković Hadžić, Stefan
AU  - Božunović, Jelena
AU  - Dmitrović, Slavica
AU  - Nestorović Živković, Jasmina
AU  - Gašić, Uroš
AU  - Milutinović, Milica
AU  - Petrović, Luka
AU  - Mišić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5571
AB  - Because of their sessile lifestyle, plants have been constantly facing ultraviolet (UV-B) radiation,
a type of abiotic stress which affects their overall growth, development, physiology, and metabolism. Qualitative and quantitative content of specialized metabolites, chemical compounds that
allow plants to adapt to specific conditions of the environment, is also affected. Nepeta nuda L.
(fam. Lamiaceae) is characterized by a significant amount of iridoids, monoterpenoid compounds
with prominent pharmacological effects. The aim of this study was to detect changes in the content of major iridoids (1,5,9-epideoxyloganic acid (1,5,9-eDLA), nepetalactone (NL), and dehydronepetalactone (DNL)) in N. nuda plants after exposure to UV-B radiation (during 30 and 60 min),
in parallel with expression analysis of genes participating in the biosynthetic pathway of iridoids
(NnGAPDH, NnGPPS, NnGES, NnG8O, Nn8HGO, NnIS, NnNEPS). After both UV-B treatments, the gene
GPPS showed the highest increase in gene expression, relative to control plants, while the gene
G8O showed the highest decrease in expression level. At the same time, UHPLC/DAD/(±)HESI-MS2
analysis revealed an increase in the concentration of 1,5,9-eDLA and DNL following the exposure
of plants to UV-B radiation for 60 minutes. In the case of a 30-minute treatment, there was an
increase in the concentration of eDLA, while the concentration of DNL was reduced, relative to
non-treated plants. The present study brings novel insights into the molecular background of
iridoid biosynthesis in UV-B stressed plants, which could help in optimizing the elicitation procedures and thus accumulation of bioactive compounds in N. nuda.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - UV-B radiation-elicitated biosynthesis of iridoids in leaves of in vitro grown Nepeta nuda L.
SP  - 85
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5571
ER  - 

@conference{
author = "Marković, Tijana and Aničić, Neda and Marković Hadžić, Stefan and Božunović, Jelena and Dmitrović, Slavica and Nestorović Živković, Jasmina and Gašić, Uroš and Milutinović, Milica and Petrović, Luka and Mišić, Danijela",
year = "2022",
abstract = "Because of their sessile lifestyle, plants have been constantly facing ultraviolet (UV-B) radiation,
a type of abiotic stress which affects their overall growth, development, physiology, and metabolism. Qualitative and quantitative content of specialized metabolites, chemical compounds that
allow plants to adapt to specific conditions of the environment, is also affected. Nepeta nuda L.
(fam. Lamiaceae) is characterized by a significant amount of iridoids, monoterpenoid compounds
with prominent pharmacological effects. The aim of this study was to detect changes in the content of major iridoids (1,5,9-epideoxyloganic acid (1,5,9-eDLA), nepetalactone (NL), and dehydronepetalactone (DNL)) in N. nuda plants after exposure to UV-B radiation (during 30 and 60 min),
in parallel with expression analysis of genes participating in the biosynthetic pathway of iridoids
(NnGAPDH, NnGPPS, NnGES, NnG8O, Nn8HGO, NnIS, NnNEPS). After both UV-B treatments, the gene
GPPS showed the highest increase in gene expression, relative to control plants, while the gene
G8O showed the highest decrease in expression level. At the same time, UHPLC/DAD/(±)HESI-MS2
analysis revealed an increase in the concentration of 1,5,9-eDLA and DNL following the exposure
of plants to UV-B radiation for 60 minutes. In the case of a 30-minute treatment, there was an
increase in the concentration of eDLA, while the concentration of DNL was reduced, relative to
non-treated plants. The present study brings novel insights into the molecular background of
iridoid biosynthesis in UV-B stressed plants, which could help in optimizing the elicitation procedures and thus accumulation of bioactive compounds in N. nuda.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "UV-B radiation-elicitated biosynthesis of iridoids in leaves of in vitro grown Nepeta nuda L.",
pages = "85",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5571"
}

Marković, T., Aničić, N., Marković Hadžić, S., Božunović, J., Dmitrović, S., Nestorović Živković, J., Gašić, U., Milutinović, M., Petrović, L.,& Mišić, D.. (2022). UV-B radiation-elicitated biosynthesis of iridoids in leaves of in vitro grown Nepeta nuda L.. in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 85.
https://hdl.handle.net/21.15107/rcub_ibiss_5571

Marković T, Aničić N, Marković Hadžić S, Božunović J, Dmitrović S, Nestorović Živković J, Gašić U, Milutinović M, Petrović L, Mišić D. UV-B radiation-elicitated biosynthesis of iridoids in leaves of in vitro grown Nepeta nuda L.. in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:85.
https://hdl.handle.net/21.15107/rcub_ibiss_5571 .

Marković, Tijana, Aničić, Neda, Marković Hadžić, Stefan, Božunović, Jelena, Dmitrović, Slavica, Nestorović Živković, Jasmina, Gašić, Uroš, Milutinović, Milica, Petrović, Luka, Mišić, Danijela, "UV-B radiation-elicitated biosynthesis of iridoids in leaves of in vitro grown Nepeta nuda L." in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):85,
https://hdl.handle.net/21.15107/rcub_ibiss_5571 .