TY  - JOUR
AU  - Skorić, Marijana
AU  - Živković, Suzana
AU  - Savić, Jelena
AU  - Šiler, Branislav
AU  - Sabovljević, Aneta
AU  - Todorović, Slađana
AU  - Grubišić, Dragoljub
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4331
AB  - A rapid, one-step method for direct leafy and rooted bulblets regeneration of Lilium martagon var. cattaniae Vis. was established using seeds as the starting explants for in vitro culture initiation. Adventitious bulblets were regenerated from one scale explants on MS (Murashige and Skoog, 1962) medium supplied with various concentrations of plant growth regulators. The most efficient medium for multiplication was MS supplemented with 0.2 mg/l 6-benzylaminopurine (BAP) and indole-3-acetic acid (IAA) ranging from 0.25 to 2 mg/l. On average, five bulblets were obtained per explant after six weeks in culture. Upon rooting, about 200 plantlets were successfully hardened in the green house with a 95% survival rate. Preliminary experiments indicated that the plantlets from the greenhouse could be successfully used for field cultivation. In vitro propagation of this endemic and protected plant species is of great importance for germplasm conservation.
T2  - African Journal of Biotechnology
T1  - Efficient one-step tissue culture protocol for propagation of endemic plant, Lilium martagon var. cattaniae Vis.
IS  - 8
VL  - 11
DO  - 10.5897/AJB11.2076
SP  - 1862
EP  - 1867
ER  - 

@article{
author = "Skorić, Marijana and Živković, Suzana and Savić, Jelena and Šiler, Branislav and Sabovljević, Aneta and Todorović, Slađana and Grubišić, Dragoljub",
year = "2012",
abstract = "A rapid, one-step method for direct leafy and rooted bulblets regeneration of Lilium martagon var. cattaniae Vis. was established using seeds as the starting explants for in vitro culture initiation. Adventitious bulblets were regenerated from one scale explants on MS (Murashige and Skoog, 1962) medium supplied with various concentrations of plant growth regulators. The most efficient medium for multiplication was MS supplemented with 0.2 mg/l 6-benzylaminopurine (BAP) and indole-3-acetic acid (IAA) ranging from 0.25 to 2 mg/l. On average, five bulblets were obtained per explant after six weeks in culture. Upon rooting, about 200 plantlets were successfully hardened in the green house with a 95% survival rate. Preliminary experiments indicated that the plantlets from the greenhouse could be successfully used for field cultivation. In vitro propagation of this endemic and protected plant species is of great importance for germplasm conservation.",
journal = "African Journal of Biotechnology",
title = "Efficient one-step tissue culture protocol for propagation of endemic plant, Lilium martagon var. cattaniae Vis.",
number = "8",
volume = "11",
doi = "10.5897/AJB11.2076",
pages = "1862-1867"
}

Skorić, M., Živković, S., Savić, J., Šiler, B., Sabovljević, A., Todorović, S.,& Grubišić, D.. (2012). Efficient one-step tissue culture protocol for propagation of endemic plant, Lilium martagon var. cattaniae Vis.. in African Journal of Biotechnology, 11(8), 1862-1867.
https://doi.org/10.5897/AJB11.2076

Skorić M, Živković S, Savić J, Šiler B, Sabovljević A, Todorović S, Grubišić D. Efficient one-step tissue culture protocol for propagation of endemic plant, Lilium martagon var. cattaniae Vis.. in African Journal of Biotechnology. 2012;11(8):1862-1867.
doi:10.5897/AJB11.2076 .

Skorić, Marijana, Živković, Suzana, Savić, Jelena, Šiler, Branislav, Sabovljević, Aneta, Todorović, Slađana, Grubišić, Dragoljub, "Efficient one-step tissue culture protocol for propagation of endemic plant, Lilium martagon var. cattaniae Vis." in African Journal of Biotechnology, 11, no. 8 (2012):1862-1867,
https://doi.org/10.5897/AJB11.2076 . .

TY  - JOUR
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Džamić, Ana M
AU  - Ćirić, Ana
AU  - Ristić, Mihailo S.
AU  - Grubišić, Dragoljub
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1254
AB  - The following study deals with the chemical composition, antimicrobial activity of essential oils of Vitex agnus-castus L and their main constituents in vitro and in vivo. The main compounds in the oil of unripe fruits were sabinene (17.8%) and 1,8-cineole (17.5%), while in the oil of the ripe fruits dominant compounds were 1,8-cineole (16.3%) and sabinene (13.4%). The leaves oil contained an abundance of 1,8-cineole (22.0%), as well. All of the oils tested were rich sources of alpha-pinene (12.2%, 9.4% and 9.4%, respectively). Antimicrobial activity was tested using bacterial and fungal strains by the microdilution method. Using the same technique 1,8-cineole and alpha-pinene showed very high antimicrobial potency as well. As 1,8-cineole was the predominant constituent of the oils, we have chosen to test it further in an in vivo experiment. Randomly chosen apples were treated with 1,8-cineol solution and infected with Aspergillus niger in order to provoke Aspergillus rot in apples. Disease incidence was recorded. (C) 2011 Elsevier Ltd. All rights reserved.
T2  - Food Chemistry
T1  - Chemical composition and antimicrobial activity of Vitex agnus-castus L. fruits and leaves essential oils
IS  - 4
VL  - 128
EP  - 1022
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1254
ER  - 

@article{
author = "Stojković, Dejan and Soković, Marina and Glamočlija, Jasmina and Džamić, Ana M and Ćirić, Ana and Ristić, Mihailo S. and Grubišić, Dragoljub",
year = "2011",
abstract = "The following study deals with the chemical composition, antimicrobial activity of essential oils of Vitex agnus-castus L and their main constituents in vitro and in vivo. The main compounds in the oil of unripe fruits were sabinene (17.8%) and 1,8-cineole (17.5%), while in the oil of the ripe fruits dominant compounds were 1,8-cineole (16.3%) and sabinene (13.4%). The leaves oil contained an abundance of 1,8-cineole (22.0%), as well. All of the oils tested were rich sources of alpha-pinene (12.2%, 9.4% and 9.4%, respectively). Antimicrobial activity was tested using bacterial and fungal strains by the microdilution method. Using the same technique 1,8-cineole and alpha-pinene showed very high antimicrobial potency as well. As 1,8-cineole was the predominant constituent of the oils, we have chosen to test it further in an in vivo experiment. Randomly chosen apples were treated with 1,8-cineol solution and infected with Aspergillus niger in order to provoke Aspergillus rot in apples. Disease incidence was recorded. (C) 2011 Elsevier Ltd. All rights reserved.",
journal = "Food Chemistry",
title = "Chemical composition and antimicrobial activity of Vitex agnus-castus L. fruits and leaves essential oils",
number = "4",
volume = "128",
pages = "1022",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1254"
}

Stojković, D., Soković, M., Glamočlija, J., Džamić, A. M., Ćirić, A., Ristić, M. S.,& Grubišić, D.. (2011). Chemical composition and antimicrobial activity of Vitex agnus-castus L. fruits and leaves essential oils. in Food Chemistry, 128(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1254

Stojković D, Soković M, Glamočlija J, Džamić AM, Ćirić A, Ristić MS, Grubišić D. Chemical composition and antimicrobial activity of Vitex agnus-castus L. fruits and leaves essential oils. in Food Chemistry. 2011;128(4):null-1022.
https://hdl.handle.net/21.15107/rcub_ibiss_1254 .

Stojković, Dejan, Soković, Marina, Glamočlija, Jasmina, Džamić, Ana M, Ćirić, Ana, Ristić, Mihailo S., Grubišić, Dragoljub, "Chemical composition and antimicrobial activity of Vitex agnus-castus L. fruits and leaves essential oils" in Food Chemistry, 128, no. 4 (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1254 .

TY  - CONF
AU  - Dragićević, Milan
AU  - Bogdanović, Milica
AU  - Todorović, Slađana
AU  - Ghalawenji, Nabil
AU  - Simonović, Ana
AU  - Grubišić, Dragoljub
PY  - 2011
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6177
AB  - Glutamine synthetase (GS, EC 6.3.1.2) is a key enzyme of ammonium assimilation and recycling in plants.
In Arabidopsis, five GLN1 genes encode cytosolic (GS1) isoforms which combine to form, at least nine multimeric GS holoenzymes with overlapping expression. To investigate the role of each isoform, we used respective T-DNA insertion (SALK and SAIL) lines, obtained from The European Arabidopsis Stock Centre. The lines were picked so that the insertion is located in the exons of GLN genes, hopefully preventing the functionality of the transcripts. The plants carrying the T-DNA insertion were selected on kanamycin (for SALK lines carrying the neo gene) or phosphinotricin (for SAIL lines carrying the bar gene). The surviving plants were screened with a three-primer PCR protocol which allows for differentiation of wild type, homozygous and heterozygous plants. For monitoring the transcription of wild type and mutant alleles, the fast, simple and cost-efficient Touch and Go technique was used. So far, homozygous knockout mutants of two high expressed GS isoforms (GLN1;2 and GLN1;3) have been identified.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Programme and Abstracts: 19th Symposium of the Serbian Plant Physiology Society; 2011 Jun 13-15; Banja Vrujci, Serbia
T1  - Screening for glutamine synthetase knockout mutants in Arabidopsis
SP  - 49
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6177
ER  - 

@conference{
author = "Dragićević, Milan and Bogdanović, Milica and Todorović, Slađana and Ghalawenji, Nabil and Simonović, Ana and Grubišić, Dragoljub",
year = "2011",
abstract = "Glutamine synthetase (GS, EC 6.3.1.2) is a key enzyme of ammonium assimilation and recycling in plants.
In Arabidopsis, five GLN1 genes encode cytosolic (GS1) isoforms which combine to form, at least nine multimeric GS holoenzymes with overlapping expression. To investigate the role of each isoform, we used respective T-DNA insertion (SALK and SAIL) lines, obtained from The European Arabidopsis Stock Centre. The lines were picked so that the insertion is located in the exons of GLN genes, hopefully preventing the functionality of the transcripts. The plants carrying the T-DNA insertion were selected on kanamycin (for SALK lines carrying the neo gene) or phosphinotricin (for SAIL lines carrying the bar gene). The surviving plants were screened with a three-primer PCR protocol which allows for differentiation of wild type, homozygous and heterozygous plants. For monitoring the transcription of wild type and mutant alleles, the fast, simple and cost-efficient Touch and Go technique was used. So far, homozygous knockout mutants of two high expressed GS isoforms (GLN1;2 and GLN1;3) have been identified.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Programme and Abstracts: 19th Symposium of the Serbian Plant Physiology Society; 2011 Jun 13-15; Banja Vrujci, Serbia",
title = "Screening for glutamine synthetase knockout mutants in Arabidopsis",
pages = "49",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6177"
}

Dragićević, M., Bogdanović, M., Todorović, S., Ghalawenji, N., Simonović, A.,& Grubišić, D.. (2011). Screening for glutamine synthetase knockout mutants in Arabidopsis. in Programme and Abstracts: 19th Symposium of the Serbian Plant Physiology Society; 2011 Jun 13-15; Banja Vrujci, Serbia
Belgrade: Serbian Plant Physiology Society., 49.
https://hdl.handle.net/21.15107/rcub_ibiss_6177

Dragićević M, Bogdanović M, Todorović S, Ghalawenji N, Simonović A, Grubišić D. Screening for glutamine synthetase knockout mutants in Arabidopsis. in Programme and Abstracts: 19th Symposium of the Serbian Plant Physiology Society; 2011 Jun 13-15; Banja Vrujci, Serbia. 2011;:49.
https://hdl.handle.net/21.15107/rcub_ibiss_6177 .

Dragićević, Milan, Bogdanović, Milica, Todorović, Slađana, Ghalawenji, Nabil, Simonović, Ana, Grubišić, Dragoljub, "Screening for glutamine synthetase knockout mutants in Arabidopsis" in Programme and Abstracts: 19th Symposium of the Serbian Plant Physiology Society; 2011 Jun 13-15; Banja Vrujci, Serbia (2011):49,
https://hdl.handle.net/21.15107/rcub_ibiss_6177 .

TY  - CONF
AU  - Todorović, Slađana
AU  - Banjanac, Tijana
AU  - Bogdanović, Milica
AU  - Dragićević, Milan
AU  - Simonović, Ana
AU  - Giba, Zlatko
AU  - Grubišić, Dragoljub
PY  - 2011
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6176
AB  - Many chicory varieties (Cichorium intybus L., Asteraceae) are important agricultural and medicinal plants used throughout Europe. The bitter taste of chicory is associated with the presence of large quantities of sesquiterpene lactones. Some of the guanolides isolated from chicory, play role as antifeedants and phytoalexins, and possess cytotoxic activity towards cultured cancer cells, while the root extracts have anti-inflammatory and hepatoprotective activities. Hairy roots obtained by A. rhizogenes transformation grow fast and do not require hormones in the medium. The greatest advantage of hairy roots is that they often exhibit greater biosynthetic capacity for secondary metabolite
production compared to the mother plants. The main scope of our investigation was to optimize protocols for transformation of two chicory cultivars, C. intybus blue and C. intybus catalogna, with A. rhizogenes bearing 35S-GUS reporter vector and to establish optimal conditions for hairy roots growth. Transformation was confirmed in 7 C. intybus blue and in 5 C. intybus catalogna clones by PCR with GUS-specific primers. The GUS expression was confirmed in 6 C. intybus blue and 4 C. intybus catalogna clones by RT-PCR and histochemical GUS staining. Two of the transformed clones of C. intybus catalogna explained the greatest biomass production and growth potential. The established transformation protocol will be the basis for future planned transformation experiments and for the large-scale production of target secundary metabolites using hairy root cultures.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Programme and Abstracts: 19th Symposium of the Serbian Plant Physiology Society; 2011 Jun 13-15; Banja Vrujci, Serbia
T1  - In vitro culture establishment and biomass production of Cichorium intybus L. hairy roots
SP  - 79
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6176
ER  - 

@conference{
author = "Todorović, Slađana and Banjanac, Tijana and Bogdanović, Milica and Dragićević, Milan and Simonović, Ana and Giba, Zlatko and Grubišić, Dragoljub",
year = "2011",
abstract = "Many chicory varieties (Cichorium intybus L., Asteraceae) are important agricultural and medicinal plants used throughout Europe. The bitter taste of chicory is associated with the presence of large quantities of sesquiterpene lactones. Some of the guanolides isolated from chicory, play role as antifeedants and phytoalexins, and possess cytotoxic activity towards cultured cancer cells, while the root extracts have anti-inflammatory and hepatoprotective activities. Hairy roots obtained by A. rhizogenes transformation grow fast and do not require hormones in the medium. The greatest advantage of hairy roots is that they often exhibit greater biosynthetic capacity for secondary metabolite
production compared to the mother plants. The main scope of our investigation was to optimize protocols for transformation of two chicory cultivars, C. intybus blue and C. intybus catalogna, with A. rhizogenes bearing 35S-GUS reporter vector and to establish optimal conditions for hairy roots growth. Transformation was confirmed in 7 C. intybus blue and in 5 C. intybus catalogna clones by PCR with GUS-specific primers. The GUS expression was confirmed in 6 C. intybus blue and 4 C. intybus catalogna clones by RT-PCR and histochemical GUS staining. Two of the transformed clones of C. intybus catalogna explained the greatest biomass production and growth potential. The established transformation protocol will be the basis for future planned transformation experiments and for the large-scale production of target secundary metabolites using hairy root cultures.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Programme and Abstracts: 19th Symposium of the Serbian Plant Physiology Society; 2011 Jun 13-15; Banja Vrujci, Serbia",
title = "In vitro culture establishment and biomass production of Cichorium intybus L. hairy roots",
pages = "79",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6176"
}

Todorović, S., Banjanac, T., Bogdanović, M., Dragićević, M., Simonović, A., Giba, Z.,& Grubišić, D.. (2011). In vitro culture establishment and biomass production of Cichorium intybus L. hairy roots. in Programme and Abstracts: 19th Symposium of the Serbian Plant Physiology Society; 2011 Jun 13-15; Banja Vrujci, Serbia
Belgrade: Serbian Plant Physiology Society., 79.
https://hdl.handle.net/21.15107/rcub_ibiss_6176

Todorović S, Banjanac T, Bogdanović M, Dragićević M, Simonović A, Giba Z, Grubišić D. In vitro culture establishment and biomass production of Cichorium intybus L. hairy roots. in Programme and Abstracts: 19th Symposium of the Serbian Plant Physiology Society; 2011 Jun 13-15; Banja Vrujci, Serbia. 2011;:79.
https://hdl.handle.net/21.15107/rcub_ibiss_6176 .

Todorović, Slađana, Banjanac, Tijana, Bogdanović, Milica, Dragićević, Milan, Simonović, Ana, Giba, Zlatko, Grubišić, Dragoljub, "In vitro culture establishment and biomass production of Cichorium intybus L. hairy roots" in Programme and Abstracts: 19th Symposium of the Serbian Plant Physiology Society; 2011 Jun 13-15; Banja Vrujci, Serbia (2011):79,
https://hdl.handle.net/21.15107/rcub_ibiss_6176 .

TY  - JOUR
AU  - Bogdanović, Milica
AU  - Ilić, Milena D
AU  - Živković, Suzana
AU  - Sabovljević, Aneta
AU  - Grubišić, Dragoljub
AU  - Sabovljević, Marko S
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1320
AB  - Salt demonstrates various osmotic and ionic effects on vascular plant growth, development and function, but very few data can be found on how salt affects non-tracheophytes. To explore this, gametophytes of two moss - Bryum argenteum Hedw. and Atrichum undulatum (Hedw.) P. Beauv., and three fern species - Asplenium viride Britton, Ceterach officinarum DC, and Phyllitis scolopendrium (L.) Newman, were treated for 3 days with different NaCl concentrations in growth medium under in vitro controlled conditions. Subsequently, these plants recovered for 18 days on NaCl-free medium, after which the following parameters were measured for mosses: presence of secondary protonema and shoots, protonemal radius and index of multiplication. Survival, chlorophyll a, b, total and a/b ratio were determined as well as total phenolic content, both for ferns and mosses. All species tolerated 50 and 100mMof NaCl-enriched media, quite well. On higher salt concentrations in the substrata, measured morphological parameters and chlorophyll content were reduced. In general, mosses exhibited higher NaCl tolerance than ferns. Change of phenolic content in ferns suggests these plants use antioxidative properties of phenolics as a mechanism of salt tolerance, in contrast with mosses whose phenolic content was stable.
T2  - Australian Journal of Botany
T1  - Comparative study on the effects of NaCl on selected moss and fern representatives
IS  - 8
VL  - 59
EP  - 740
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1320
ER  - 

@article{
author = "Bogdanović, Milica and Ilić, Milena D and Živković, Suzana and Sabovljević, Aneta and Grubišić, Dragoljub and Sabovljević, Marko S",
year = "2011",
abstract = "Salt demonstrates various osmotic and ionic effects on vascular plant growth, development and function, but very few data can be found on how salt affects non-tracheophytes. To explore this, gametophytes of two moss - Bryum argenteum Hedw. and Atrichum undulatum (Hedw.) P. Beauv., and three fern species - Asplenium viride Britton, Ceterach officinarum DC, and Phyllitis scolopendrium (L.) Newman, were treated for 3 days with different NaCl concentrations in growth medium under in vitro controlled conditions. Subsequently, these plants recovered for 18 days on NaCl-free medium, after which the following parameters were measured for mosses: presence of secondary protonema and shoots, protonemal radius and index of multiplication. Survival, chlorophyll a, b, total and a/b ratio were determined as well as total phenolic content, both for ferns and mosses. All species tolerated 50 and 100mMof NaCl-enriched media, quite well. On higher salt concentrations in the substrata, measured morphological parameters and chlorophyll content were reduced. In general, mosses exhibited higher NaCl tolerance than ferns. Change of phenolic content in ferns suggests these plants use antioxidative properties of phenolics as a mechanism of salt tolerance, in contrast with mosses whose phenolic content was stable.",
journal = "Australian Journal of Botany",
title = "Comparative study on the effects of NaCl on selected moss and fern representatives",
number = "8",
volume = "59",
pages = "740",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1320"
}

Bogdanović, M., Ilić, M. D., Živković, S., Sabovljević, A., Grubišić, D.,& Sabovljević, M. S.. (2011). Comparative study on the effects of NaCl on selected moss and fern representatives. in Australian Journal of Botany, 59(8).
https://hdl.handle.net/21.15107/rcub_ibiss_1320

Bogdanović M, Ilić MD, Živković S, Sabovljević A, Grubišić D, Sabovljević MS. Comparative study on the effects of NaCl on selected moss and fern representatives. in Australian Journal of Botany. 2011;59(8):null-740.
https://hdl.handle.net/21.15107/rcub_ibiss_1320 .

Bogdanović, Milica, Ilić, Milena D, Živković, Suzana, Sabovljević, Aneta, Grubišić, Dragoljub, Sabovljević, Marko S, "Comparative study on the effects of NaCl on selected moss and fern representatives" in Australian Journal of Botany, 59, no. 8 (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1320 .

TY  - CONF
AU  - Bogdanović, Milica
AU  - Dragićević, Milan
AU  - Simonović, Ana
AU  - Tanić, Nikola
AU  - Mišić, Danijela
AU  - Grubišić, Dragoljub
PY  - 2010
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6170
AB  - 18S rRNA is widely used as a housekeeping gene in expression studies, including end-point PCR, Northern blotting and real-time experiments. There are, however, two disadvantages (and two points of error introduction) in using 18S rRNA as a reference gene. First, 18S has no poly-A tail, so it is traditionally reverse transcribed with either specific primers or random hexamers, e.g. separately from poly(dT)-primed transcripts. Secondly, due to its abundance, estimated to be ≈2.5 x 105 higher than an averagely expressed mRNA, the 18S cDNA must be diluted 1000-10.000 times to be comparable to the tested genes. Hereby we present that 18S rRNA can be successfully reverse transcribed using poly(dT)18 primers in all representative plant species tested: a moss, Physcomitrella patens, a fern, Adiantum capillus-veneris, a monocot, Zea mays and two dicots - Centaurium erythraea and Arabidopsis thaliana. The efficiency of the poly(dT)-primed RT was confirmed by end-point PCR and quantified by qPCR with 18S-specific primers. The efficiency of RT (miss)priming using poly(dT)18 was 90-900 times less effective than specific priming, depending on the species’ 18S sequence. Using poly(dT)18 primers for 18S RT circumvents both above mentioned obstacles, eliminating not only the need for separate RT mixtures, but also the need for 18S cDNA dilution. For theoretical rather than practical reason, all other homopolymers, poly(dA)18, poly(dC)18 and poly(dG)18, were also tested, and all were efficient as 18S RT primers. Poly(dC)18 was as efficient as specific primer, and the only one that interfered with PCR, giving species-specific pattern of products in addition to the product of expected size. The comparative results of RT-PCR and RT-qPCR of 18S rRNA with different RT primers (all homopolymers, specific primers and random hexamers) and specific PCR primers, for all five species are presented. The possible mechanism of homopolymeric (miss)priming is discussed.
PB  - European Molecular Biology Laboratory
C3  - 9th EMBL Conference on Transcription and Chromatin; 2010 Aug 28-31; Heidelberg, Germany
T1  - Homopolymeric priming of 18S rRNA in reverse transcription
SP  - 108
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6170
ER  - 

@conference{
author = "Bogdanović, Milica and Dragićević, Milan and Simonović, Ana and Tanić, Nikola and Mišić, Danijela and Grubišić, Dragoljub",
year = "2010",
abstract = "18S rRNA is widely used as a housekeeping gene in expression studies, including end-point PCR, Northern blotting and real-time experiments. There are, however, two disadvantages (and two points of error introduction) in using 18S rRNA as a reference gene. First, 18S has no poly-A tail, so it is traditionally reverse transcribed with either specific primers or random hexamers, e.g. separately from poly(dT)-primed transcripts. Secondly, due to its abundance, estimated to be ≈2.5 x 105 higher than an averagely expressed mRNA, the 18S cDNA must be diluted 1000-10.000 times to be comparable to the tested genes. Hereby we present that 18S rRNA can be successfully reverse transcribed using poly(dT)18 primers in all representative plant species tested: a moss, Physcomitrella patens, a fern, Adiantum capillus-veneris, a monocot, Zea mays and two dicots - Centaurium erythraea and Arabidopsis thaliana. The efficiency of the poly(dT)-primed RT was confirmed by end-point PCR and quantified by qPCR with 18S-specific primers. The efficiency of RT (miss)priming using poly(dT)18 was 90-900 times less effective than specific priming, depending on the species’ 18S sequence. Using poly(dT)18 primers for 18S RT circumvents both above mentioned obstacles, eliminating not only the need for separate RT mixtures, but also the need for 18S cDNA dilution. For theoretical rather than practical reason, all other homopolymers, poly(dA)18, poly(dC)18 and poly(dG)18, were also tested, and all were efficient as 18S RT primers. Poly(dC)18 was as efficient as specific primer, and the only one that interfered with PCR, giving species-specific pattern of products in addition to the product of expected size. The comparative results of RT-PCR and RT-qPCR of 18S rRNA with different RT primers (all homopolymers, specific primers and random hexamers) and specific PCR primers, for all five species are presented. The possible mechanism of homopolymeric (miss)priming is discussed.",
publisher = "European Molecular Biology Laboratory",
journal = "9th EMBL Conference on Transcription and Chromatin; 2010 Aug 28-31; Heidelberg, Germany",
title = "Homopolymeric priming of 18S rRNA in reverse transcription",
pages = "108",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6170"
}

Bogdanović, M., Dragićević, M., Simonović, A., Tanić, N., Mišić, D.,& Grubišić, D.. (2010). Homopolymeric priming of 18S rRNA in reverse transcription. in 9th EMBL Conference on Transcription and Chromatin; 2010 Aug 28-31; Heidelberg, Germany
European Molecular Biology Laboratory., 108.
https://hdl.handle.net/21.15107/rcub_ibiss_6170

Bogdanović M, Dragićević M, Simonović A, Tanić N, Mišić D, Grubišić D. Homopolymeric priming of 18S rRNA in reverse transcription. in 9th EMBL Conference on Transcription and Chromatin; 2010 Aug 28-31; Heidelberg, Germany. 2010;:108.
https://hdl.handle.net/21.15107/rcub_ibiss_6170 .

Bogdanović, Milica, Dragićević, Milan, Simonović, Ana, Tanić, Nikola, Mišić, Danijela, Grubišić, Dragoljub, "Homopolymeric priming of 18S rRNA in reverse transcription" in 9th EMBL Conference on Transcription and Chromatin; 2010 Aug 28-31; Heidelberg, Germany (2010):108,
https://hdl.handle.net/21.15107/rcub_ibiss_6170 .

TY  - JOUR
AU  - Todorović, Slađana
AU  - Božić, Dragana
AU  - Simonović, Ana
AU  - Filipović, Biljana
AU  - Dragičević, Milan B.
AU  - Giba, Zlatko
AU  - Grubišić, Dragoljub
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1353
AB  - The Empress tree (Paulownia tomentosa Steud.) is a fast growing deciduous tree originating from East Asia. It is grown as an ornamental plant, but can also be used as industrial wood. However, in some parts of North America, this species is considered invasive, with high impact on autochthonous populations. We have investigated whether post-fire expansion of this species can be explained at the germination level by analyzing the interaction between physical (light and temperature) and chemical (smoke and nitrogenous compounds) fire-related cues in the regulation of P. tomentosa germination. Light is the key environmental signal in the induction of germination of positively photoblastic P. tomentosa seeds. Smoke, applied in the form of extract (liquid smoke [LS]), stimulates light-induced germination if applied during imbibition, or shortly after the inductive irradiation. This stimulatory effect of LS can be effectively prevented by far-red light, suggesting that the mechanism of smoke action is phytochrome related. The LS had no effect on the germination percentage when applied during the phase of radicle protrusion. However, LS reduced radicle elongation and slowed its protrusion. The LS had no effect on the optimal temperature range of germination (23-27 degrees C). The combined action of LS and KNO(3) in light-induced germination was more effective then when each chemical was applied separately. It can be concluded that all tested components of fire, except elevated temperature, operate to stimulate germination of P. tomentosa seeds, suggesting that the post-fire invasive potential of this species can, at least in part, be explained at the germination level.
T2  - Plant Species Biology
T1  - Interaction of fire-related cues in seed germination of the potentially invasive species Paulownia tomentosa Steud
IS  - 3
VL  - 25
EP  - 202
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1353
ER  - 

@article{
author = "Todorović, Slađana and Božić, Dragana and Simonović, Ana and Filipović, Biljana and Dragičević, Milan B. and Giba, Zlatko and Grubišić, Dragoljub",
year = "2010",
abstract = "The Empress tree (Paulownia tomentosa Steud.) is a fast growing deciduous tree originating from East Asia. It is grown as an ornamental plant, but can also be used as industrial wood. However, in some parts of North America, this species is considered invasive, with high impact on autochthonous populations. We have investigated whether post-fire expansion of this species can be explained at the germination level by analyzing the interaction between physical (light and temperature) and chemical (smoke and nitrogenous compounds) fire-related cues in the regulation of P. tomentosa germination. Light is the key environmental signal in the induction of germination of positively photoblastic P. tomentosa seeds. Smoke, applied in the form of extract (liquid smoke [LS]), stimulates light-induced germination if applied during imbibition, or shortly after the inductive irradiation. This stimulatory effect of LS can be effectively prevented by far-red light, suggesting that the mechanism of smoke action is phytochrome related. The LS had no effect on the germination percentage when applied during the phase of radicle protrusion. However, LS reduced radicle elongation and slowed its protrusion. The LS had no effect on the optimal temperature range of germination (23-27 degrees C). The combined action of LS and KNO(3) in light-induced germination was more effective then when each chemical was applied separately. It can be concluded that all tested components of fire, except elevated temperature, operate to stimulate germination of P. tomentosa seeds, suggesting that the post-fire invasive potential of this species can, at least in part, be explained at the germination level.",
journal = "Plant Species Biology",
title = "Interaction of fire-related cues in seed germination of the potentially invasive species Paulownia tomentosa Steud",
number = "3",
volume = "25",
pages = "202",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1353"
}

Todorović, S., Božić, D., Simonović, A., Filipović, B., Dragičević, M. B., Giba, Z.,& Grubišić, D.. (2010). Interaction of fire-related cues in seed germination of the potentially invasive species Paulownia tomentosa Steud. in Plant Species Biology, 25(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1353

Todorović S, Božić D, Simonović A, Filipović B, Dragičević MB, Giba Z, Grubišić D. Interaction of fire-related cues in seed germination of the potentially invasive species Paulownia tomentosa Steud. in Plant Species Biology. 2010;25(3):null-202.
https://hdl.handle.net/21.15107/rcub_ibiss_1353 .

Todorović, Slađana, Božić, Dragana, Simonović, Ana, Filipović, Biljana, Dragičević, Milan B., Giba, Zlatko, Grubišić, Dragoljub, "Interaction of fire-related cues in seed germination of the potentially invasive species Paulownia tomentosa Steud" in Plant Species Biology, 25, no. 3 (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1353 .

TY  - JOUR
AU  - Soković, Marina
AU  - Stojković, Dejan
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana
AU  - Ristić, Mihailo S.
AU  - Grubišić, Dragoljub
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1488
AB  - The compositions of the essential oils of wild Daucus carota L. (Apiaceae) from the ripe fruits, unripe fruits, flowers, root, leaves, and stem, collected in Serbia, were analyzed by GC and GUMS. The oils from all samples contained alpha-pinene (7.05-51.23%) and sabinene (2.68-36.69%) as major constituents. The other dominant compound in ripe and unripe fruits essential oil was alpha-muurolene (8.23-10.97%). Antibacterial and antifungal properties of these oils, against eight bacterial and eight fungal strains, were tested by a microdilution technique. The most prominent biological activity was achieved by isolated essential oils of ripe and unripe fruits of D. carota.
T2  - Pharmaceutical Biology
T1  - Susceptibility of pathogenic bacteria and fungi to essential oils of wild Daucus carota
IS  - 1
VL  - 47
EP  - 43
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1488
ER  - 

@article{
author = "Soković, Marina and Stojković, Dejan and Glamočlija, Jasmina and Ćirić, Ana and Ristić, Mihailo S. and Grubišić, Dragoljub",
year = "2009",
abstract = "The compositions of the essential oils of wild Daucus carota L. (Apiaceae) from the ripe fruits, unripe fruits, flowers, root, leaves, and stem, collected in Serbia, were analyzed by GC and GUMS. The oils from all samples contained alpha-pinene (7.05-51.23%) and sabinene (2.68-36.69%) as major constituents. The other dominant compound in ripe and unripe fruits essential oil was alpha-muurolene (8.23-10.97%). Antibacterial and antifungal properties of these oils, against eight bacterial and eight fungal strains, were tested by a microdilution technique. The most prominent biological activity was achieved by isolated essential oils of ripe and unripe fruits of D. carota.",
journal = "Pharmaceutical Biology",
title = "Susceptibility of pathogenic bacteria and fungi to essential oils of wild Daucus carota",
number = "1",
volume = "47",
pages = "43",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1488"
}

Soković, M., Stojković, D., Glamočlija, J., Ćirić, A., Ristić, M. S.,& Grubišić, D.. (2009). Susceptibility of pathogenic bacteria and fungi to essential oils of wild Daucus carota. in Pharmaceutical Biology, 47(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1488

Soković M, Stojković D, Glamočlija J, Ćirić A, Ristić MS, Grubišić D. Susceptibility of pathogenic bacteria and fungi to essential oils of wild Daucus carota. in Pharmaceutical Biology. 2009;47(1):null-43.
https://hdl.handle.net/21.15107/rcub_ibiss_1488 .

Soković, Marina, Stojković, Dejan, Glamočlija, Jasmina, Ćirić, Ana, Ristić, Mihailo S., Grubišić, Dragoljub, "Susceptibility of pathogenic bacteria and fungi to essential oils of wild Daucus carota" in Pharmaceutical Biology, 47, no. 1 (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1488 .

TY  - GEN
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Grubišić, Dragoljub
AU  - Vukojević, Jelena B.
AU  - Milenković, Ivanka M.
AU  - Ristić, Mihailo S.
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1469
T2  - Chemistry of Natural Compounds
T1  - Antifungal activity of Critmum maritimum essential oil and its components against mushroom pathogen Mycogone perniciosa
IS  - 1
VL  - 45
EP  - 97
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1469
ER  - 

@misc{
author = "Glamočlija, Jasmina and Soković, Marina and Grubišić, Dragoljub and Vukojević, Jelena B. and Milenković, Ivanka M. and Ristić, Mihailo S.",
year = "2009",
journal = "Chemistry of Natural Compounds",
title = "Antifungal activity of Critmum maritimum essential oil and its components against mushroom pathogen Mycogone perniciosa",
number = "1",
volume = "45",
pages = "97",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1469"
}

Glamočlija, J., Soković, M., Grubišić, D., Vukojević, J. B., Milenković, I. M.,& Ristić, M. S.. (2009). Antifungal activity of Critmum maritimum essential oil and its components against mushroom pathogen Mycogone perniciosa. in Chemistry of Natural Compounds, 45(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1469

Glamočlija J, Soković M, Grubišić D, Vukojević JB, Milenković IM, Ristić MS. Antifungal activity of Critmum maritimum essential oil and its components against mushroom pathogen Mycogone perniciosa. in Chemistry of Natural Compounds. 2009;45(1):null-97.
https://hdl.handle.net/21.15107/rcub_ibiss_1469 .

Glamočlija, Jasmina, Soković, Marina, Grubišić, Dragoljub, Vukojević, Jelena B., Milenković, Ivanka M., Ristić, Mihailo S., "Antifungal activity of Critmum maritimum essential oil and its components against mushroom pathogen Mycogone perniciosa" in Chemistry of Natural Compounds, 45, no. 1 (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1469 .

TY  - JOUR
AU  - Bogdanović, Milica
AU  - Sabovljević, Marko
AU  - Sabovljević, Aneta
AU  - Grubišić, Dragoljub
PY  - 2009
UR  - https://botanicaserbica.bio.bg.ac.rs/2009_33_1.html#a488
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6175
AB  - In this paper the effects of gypsum (CaSO 4 x 2H 2 O) in the growth medium were tested on two non-gypsophilous selected bryophyte species: Bryum argenteum Hedw. and Atrichum undulatum (Hedw.) P. Beauv. With aim to test if some bryophytes are exclusive for gypsum enriched substrates, the difference in gypsum effect on moss development were examined. In vitro cultures of two selected species were initiated from nearly mature spores within unopened capsules. Half strenght Murashige and Skoog media with added gypsum in concentrations of 50, 350 and 500mM were used to test bryophyte develepment. Plants were grown on media with gypsum for 3 days or 3 weeks to compare short and long term effect of salt exposure. Tested bryophytes, non-adapted to gypsum and exposed to various gypsum concentrations in MS medium, did not show to have a problem in surviving the new environment conditions. Moss species selected in this study and exposed to gypsum enriched MS medium showed variation in morphological parameters to some extent (index of multiplication, secondary protonemal production and survival rate) and chlorophyll content and slightly altered chlorophyll a/b ratio. In general, both tested species could survive gypsum enriched medium: secondary protonema was developed and new shoots were formed in both species, but slight differences were recorded between short and long term exposure to gypsum. Acording to data obtained, gypsum is not the stressor (at least not alone) which can separate some interesting bryophytes into the ecological group of gypsophytes.
PB  - Belgrade: Institute of Botany and Botanical Garden Jevremovac
T2  - Botanica Serbica
T1  - The influence of gypsiferous substrata on bryophyte growth: are there obligatory gypsophilous bryophytes?
IS  - 1
VL  - 33
SP  - 75
EP  - 82
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6175
ER  - 

@article{
author = "Bogdanović, Milica and Sabovljević, Marko and Sabovljević, Aneta and Grubišić, Dragoljub",
year = "2009",
abstract = "In this paper the effects of gypsum (CaSO 4 x 2H 2 O) in the growth medium were tested on two non-gypsophilous selected bryophyte species: Bryum argenteum Hedw. and Atrichum undulatum (Hedw.) P. Beauv. With aim to test if some bryophytes are exclusive for gypsum enriched substrates, the difference in gypsum effect on moss development were examined. In vitro cultures of two selected species were initiated from nearly mature spores within unopened capsules. Half strenght Murashige and Skoog media with added gypsum in concentrations of 50, 350 and 500mM were used to test bryophyte develepment. Plants were grown on media with gypsum for 3 days or 3 weeks to compare short and long term effect of salt exposure. Tested bryophytes, non-adapted to gypsum and exposed to various gypsum concentrations in MS medium, did not show to have a problem in surviving the new environment conditions. Moss species selected in this study and exposed to gypsum enriched MS medium showed variation in morphological parameters to some extent (index of multiplication, secondary protonemal production and survival rate) and chlorophyll content and slightly altered chlorophyll a/b ratio. In general, both tested species could survive gypsum enriched medium: secondary protonema was developed and new shoots were formed in both species, but slight differences were recorded between short and long term exposure to gypsum. Acording to data obtained, gypsum is not the stressor (at least not alone) which can separate some interesting bryophytes into the ecological group of gypsophytes.",
publisher = "Belgrade: Institute of Botany and Botanical Garden Jevremovac",
journal = "Botanica Serbica",
title = "The influence of gypsiferous substrata on bryophyte growth: are there obligatory gypsophilous bryophytes?",
number = "1",
volume = "33",
pages = "75-82",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6175"
}

Bogdanović, M., Sabovljević, M., Sabovljević, A.,& Grubišić, D.. (2009). The influence of gypsiferous substrata on bryophyte growth: are there obligatory gypsophilous bryophytes?. in Botanica Serbica
Belgrade: Institute of Botany and Botanical Garden Jevremovac., 33(1), 75-82.
https://hdl.handle.net/21.15107/rcub_ibiss_6175

Bogdanović M, Sabovljević M, Sabovljević A, Grubišić D. The influence of gypsiferous substrata on bryophyte growth: are there obligatory gypsophilous bryophytes?. in Botanica Serbica. 2009;33(1):75-82.
https://hdl.handle.net/21.15107/rcub_ibiss_6175 .

Bogdanović, Milica, Sabovljević, Marko, Sabovljević, Aneta, Grubišić, Dragoljub, "The influence of gypsiferous substrata on bryophyte growth: are there obligatory gypsophilous bryophytes?" in Botanica Serbica, 33, no. 1 (2009):75-82,
https://hdl.handle.net/21.15107/rcub_ibiss_6175 .

TY  - CONF
AU  - Bogdanović, Milica
AU  - Sabovljević, Aneta
AU  - Sabovljević, Marko
AU  - Dragićević, Milan
AU  - Platiša, Jelena
AU  - Grubišić, Dragoljub
PY  - 2009
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6173
AB  - Bryophytes are thought to avoid salty substrates, although in few species reported salt tolerance is greater than in non-halophyte tracheophytes. To explore bryophyte salinity tolerance, two species were grown in vitro on half-strenght MS media with added NaCl (concentrations of 100, 250 and 500 mM). Chlorophyll content was measured spectrophotometrically, and protein content, peroxidase and catalase activities were assayed both spectrophotometrically and by PAGE. Chlorophyll content in M. polymorpha decreases rapidly with rising salt concentrations approaching zero at 250 mM, while in A. undulatum it remains high at 100 mM of salt above which decreases. Protein content remains high at 100 mM of salt in both species and decreases at higher salt concentrations (in M. polymorpha several times compared to control values). Peroxidase activity is much higher in M. polymorpha and decreases slightly at high salt concentrations. Catalase activity in A.undulatum remains high at all salt concentrations, in contrast with M. polymorpha whose activity lowers considerably at 250 mM of salt.
PB  - Belgrade: University of Belgrade, Faculty of Biology
C3  - Book of abstracts: 5th Balkan Botanical Congress: the 80th anniversary of Turrill's Plant life of the Balkan peninsula; 2009 Sep 7-11; Belgrade, Serbia
T1  - Effect of NaCl on chlorophyll and protein content, and antioxidant enzymes activity in two bryophyte species – Atrichum undulatum (Hedw.) P. Beauv. and Marchantia polymorpha L.
SP  - 87
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6173
ER  - 

@conference{
author = "Bogdanović, Milica and Sabovljević, Aneta and Sabovljević, Marko and Dragićević, Milan and Platiša, Jelena and Grubišić, Dragoljub",
year = "2009",
abstract = "Bryophytes are thought to avoid salty substrates, although in few species reported salt tolerance is greater than in non-halophyte tracheophytes. To explore bryophyte salinity tolerance, two species were grown in vitro on half-strenght MS media with added NaCl (concentrations of 100, 250 and 500 mM). Chlorophyll content was measured spectrophotometrically, and protein content, peroxidase and catalase activities were assayed both spectrophotometrically and by PAGE. Chlorophyll content in M. polymorpha decreases rapidly with rising salt concentrations approaching zero at 250 mM, while in A. undulatum it remains high at 100 mM of salt above which decreases. Protein content remains high at 100 mM of salt in both species and decreases at higher salt concentrations (in M. polymorpha several times compared to control values). Peroxidase activity is much higher in M. polymorpha and decreases slightly at high salt concentrations. Catalase activity in A.undulatum remains high at all salt concentrations, in contrast with M. polymorpha whose activity lowers considerably at 250 mM of salt.",
publisher = "Belgrade: University of Belgrade, Faculty of Biology",
journal = "Book of abstracts: 5th Balkan Botanical Congress: the 80th anniversary of Turrill's Plant life of the Balkan peninsula; 2009 Sep 7-11; Belgrade, Serbia",
title = "Effect of NaCl on chlorophyll and protein content, and antioxidant enzymes activity in two bryophyte species – Atrichum undulatum (Hedw.) P. Beauv. and Marchantia polymorpha L.",
pages = "87",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6173"
}

Bogdanović, M., Sabovljević, A., Sabovljević, M., Dragićević, M., Platiša, J.,& Grubišić, D.. (2009). Effect of NaCl on chlorophyll and protein content, and antioxidant enzymes activity in two bryophyte species – Atrichum undulatum (Hedw.) P. Beauv. and Marchantia polymorpha L.. in Book of abstracts: 5th Balkan Botanical Congress: the 80th anniversary of Turrill's Plant life of the Balkan peninsula; 2009 Sep 7-11; Belgrade, Serbia
Belgrade: University of Belgrade, Faculty of Biology., 87.
https://hdl.handle.net/21.15107/rcub_ibiss_6173

Bogdanović M, Sabovljević A, Sabovljević M, Dragićević M, Platiša J, Grubišić D. Effect of NaCl on chlorophyll and protein content, and antioxidant enzymes activity in two bryophyte species – Atrichum undulatum (Hedw.) P. Beauv. and Marchantia polymorpha L.. in Book of abstracts: 5th Balkan Botanical Congress: the 80th anniversary of Turrill's Plant life of the Balkan peninsula; 2009 Sep 7-11; Belgrade, Serbia. 2009;:87.
https://hdl.handle.net/21.15107/rcub_ibiss_6173 .

Bogdanović, Milica, Sabovljević, Aneta, Sabovljević, Marko, Dragićević, Milan, Platiša, Jelena, Grubišić, Dragoljub, "Effect of NaCl on chlorophyll and protein content, and antioxidant enzymes activity in two bryophyte species – Atrichum undulatum (Hedw.) P. Beauv. and Marchantia polymorpha L." in Book of abstracts: 5th Balkan Botanical Congress: the 80th anniversary of Turrill's Plant life of the Balkan peninsula; 2009 Sep 7-11; Belgrade, Serbia (2009):87,
https://hdl.handle.net/21.15107/rcub_ibiss_6173 .

TY  - CONF
AU  - Sabovljević, Marko
AU  - Sabovljević, Aneta
AU  - Vukojević, Vanja
AU  - Cvetić, Tijana
AU  - Vujičić, Milorad
AU  - Bogdanović, Milica
AU  - Grubišić, Dragoljub
PY  - 2009
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6172
AB  - Bryophytes (i.e. mosses, liverworts and hornworts) are the second biggest group of the land plants. However, due to their tiny size and low economic values, they remain out of plant scientists and conservationists interest. With aim to develop better action plans for protection and conservation of these plants, we established germaplasm collection and propagation pool for 42 species of bryophytes from wide of Europe and Macaronesia. Among these species 4 are of wide European conservation interests: Anoectangium hornschuchianum (CR), Bartramia subulata (EN), Bruchia vogesiaca (VU), Ditrichum cornubicum (EN) and 6 are regionally endangered, endemic or rare species i.e. Breutelia azorica, Campylopus oerstedianus, Cyrtomnium hymenophylloides, Ditrichum plumbicola, Oreas martiana and Sphagnum palustre. Besides, counterparts of some other rare and endangered species are used as suitable model systems to test in vitro production and propagation of endangered counterparts/species: Dicranum scoparium/D. viride/D. transsylvanicum, Cinclidotus aquaticus/C. confertus, Entostodon obtusifolius/E. hungaricus, Herzogiella seligeri/H. turfacea, Plagiomnium rostratum/P.cuspidatu/P. drummondii and others. Germaplasm collection is used for active and passive species conservation and also as valuable bryophyte gene pool and reservoir for other fundamental and applied investigation.
PB  - Belgrade: University of Belgrade, Faculty of Biology
C3  - Book of abstracts: 5th Balkan Botanical Congress: the 80th anniversary of Turrill's Plant life of the Balkan peninsula; 2009 Sep 7-11; Belgrade, Serbia
T1  - Ex situ conservation of bryophytes and bryophyte germaplasm pool
SP  - 85
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6172
ER  - 

@conference{
author = "Sabovljević, Marko and Sabovljević, Aneta and Vukojević, Vanja and Cvetić, Tijana and Vujičić, Milorad and Bogdanović, Milica and Grubišić, Dragoljub",
year = "2009",
abstract = "Bryophytes (i.e. mosses, liverworts and hornworts) are the second biggest group of the land plants. However, due to their tiny size and low economic values, they remain out of plant scientists and conservationists interest. With aim to develop better action plans for protection and conservation of these plants, we established germaplasm collection and propagation pool for 42 species of bryophytes from wide of Europe and Macaronesia. Among these species 4 are of wide European conservation interests: Anoectangium hornschuchianum (CR), Bartramia subulata (EN), Bruchia vogesiaca (VU), Ditrichum cornubicum (EN) and 6 are regionally endangered, endemic or rare species i.e. Breutelia azorica, Campylopus oerstedianus, Cyrtomnium hymenophylloides, Ditrichum plumbicola, Oreas martiana and Sphagnum palustre. Besides, counterparts of some other rare and endangered species are used as suitable model systems to test in vitro production and propagation of endangered counterparts/species: Dicranum scoparium/D. viride/D. transsylvanicum, Cinclidotus aquaticus/C. confertus, Entostodon obtusifolius/E. hungaricus, Herzogiella seligeri/H. turfacea, Plagiomnium rostratum/P.cuspidatu/P. drummondii and others. Germaplasm collection is used for active and passive species conservation and also as valuable bryophyte gene pool and reservoir for other fundamental and applied investigation.",
publisher = "Belgrade: University of Belgrade, Faculty of Biology",
journal = "Book of abstracts: 5th Balkan Botanical Congress: the 80th anniversary of Turrill's Plant life of the Balkan peninsula; 2009 Sep 7-11; Belgrade, Serbia",
title = "Ex situ conservation of bryophytes and bryophyte germaplasm pool",
pages = "85",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6172"
}

Sabovljević, M., Sabovljević, A., Vukojević, V., Cvetić, T., Vujičić, M., Bogdanović, M.,& Grubišić, D.. (2009). Ex situ conservation of bryophytes and bryophyte germaplasm pool. in Book of abstracts: 5th Balkan Botanical Congress: the 80th anniversary of Turrill's Plant life of the Balkan peninsula; 2009 Sep 7-11; Belgrade, Serbia
Belgrade: University of Belgrade, Faculty of Biology., 85.
https://hdl.handle.net/21.15107/rcub_ibiss_6172

Sabovljević M, Sabovljević A, Vukojević V, Cvetić T, Vujičić M, Bogdanović M, Grubišić D. Ex situ conservation of bryophytes and bryophyte germaplasm pool. in Book of abstracts: 5th Balkan Botanical Congress: the 80th anniversary of Turrill's Plant life of the Balkan peninsula; 2009 Sep 7-11; Belgrade, Serbia. 2009;:85.
https://hdl.handle.net/21.15107/rcub_ibiss_6172 .

Sabovljević, Marko, Sabovljević, Aneta, Vukojević, Vanja, Cvetić, Tijana, Vujičić, Milorad, Bogdanović, Milica, Grubišić, Dragoljub, "Ex situ conservation of bryophytes and bryophyte germaplasm pool" in Book of abstracts: 5th Balkan Botanical Congress: the 80th anniversary of Turrill's Plant life of the Balkan peninsula; 2009 Sep 7-11; Belgrade, Serbia (2009):85,
https://hdl.handle.net/21.15107/rcub_ibiss_6172 .

TY  - CONF
AU  - Bogdanović, Milica
AU  - Sabovljević, Aneta
AU  - Sabovljević, Marko
AU  - Grubišić, Dragoljub
PY  - 2009
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6178
AB  - Briofite su poznate kao organizmi koji izbegavaju slane supstrate a u morima ih uopšte nema.
S ciljem izučavanja odnosa briofita prema slanoj sredini, gametofiti mahovina Atrichum undulatum, Physcomitrella patens i jetrenjače Marchantia polymorpha su gajeni na MS hranljivoj podlozi sa dodatkom različitih koncentracija NaCl (50, 100, 250, 350, 500mM) tokom 3 nedelje u uslovima dugog dana (fotoperiod 16/8h). Preživljavanje i multiplikacija izdanaka se drastično smanjuju kod sve tri vrste na visokim koncentracijama soli, a produkcija biomase kod M. polymorpha gotovo eksponencijalno opada sa povećanjem koncentracije soli. P. patens dobro preživljava na koncentracijama soli nižim od 350mM. Koncentracija hlorofila a, b i ukupnog hlorofila kod sve tri ispitivane vrste opada sa porastom koncentracije NaCl, a blagi porast količine navedenih pigmenata uočava se na podlozi obogaćenoj sa 100mM NaCl. M. polymorpha vrlo loše podnosi koncentracije soli veće od 250mM u pogledu svih ispitivanih parametara.
AB  - Bryophytes are known to avoid salty substrates and do not inhabit the seas. With the purpose of studying the relation between bryophytes and salty environment, gametophytes of the two mosses - Atrichum undulatum, Physcomitrella patens and the liverwort Marchantia polymorpha were grown on MS media enriched with various concentrations of NaCl (50, 100, 250, 350, 500mM) during three weeks at 16/8h photoperiod. Survival and bud formation were drastically reduced in all three species at high salt concentrations and biomass production in M. polymorpha declined almost exponentially as salt concentration increased. P. patens survived concentrations less than 350mM salt quite well. Chlorophyll content (-a, -b and total) in all examined species declined as salt concentration increased, however a modest rise in chlorophyll content in all three species was observed when the concentration of salt in the medium was 100mM. M. polymorpha did not tolerate well concentrations above 250mM according to all the parameters observed.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Programme and Abstracts: 18th Simposium Society of Plant Physiology of Serbia; 2009 May 25-27; Vršac, Serbia
T1  - Uticaj NaCl na morfogenezu i sadržaj hlorofila tri vrste briofita u kulturi in vitro
T1  - Influence of NaCl on morphogenesis and chlorophyll content of three bryophyte species in in vitro culture
SP  - 94
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6178
ER  - 

@conference{
author = "Bogdanović, Milica and Sabovljević, Aneta and Sabovljević, Marko and Grubišić, Dragoljub",
year = "2009",
abstract = "Briofite su poznate kao organizmi koji izbegavaju slane supstrate a u morima ih uopšte nema.
S ciljem izučavanja odnosa briofita prema slanoj sredini, gametofiti mahovina Atrichum undulatum, Physcomitrella patens i jetrenjače Marchantia polymorpha su gajeni na MS hranljivoj podlozi sa dodatkom različitih koncentracija NaCl (50, 100, 250, 350, 500mM) tokom 3 nedelje u uslovima dugog dana (fotoperiod 16/8h). Preživljavanje i multiplikacija izdanaka se drastično smanjuju kod sve tri vrste na visokim koncentracijama soli, a produkcija biomase kod M. polymorpha gotovo eksponencijalno opada sa povećanjem koncentracije soli. P. patens dobro preživljava na koncentracijama soli nižim od 350mM. Koncentracija hlorofila a, b i ukupnog hlorofila kod sve tri ispitivane vrste opada sa porastom koncentracije NaCl, a blagi porast količine navedenih pigmenata uočava se na podlozi obogaćenoj sa 100mM NaCl. M. polymorpha vrlo loše podnosi koncentracije soli veće od 250mM u pogledu svih ispitivanih parametara., Bryophytes are known to avoid salty substrates and do not inhabit the seas. With the purpose of studying the relation between bryophytes and salty environment, gametophytes of the two mosses - Atrichum undulatum, Physcomitrella patens and the liverwort Marchantia polymorpha were grown on MS media enriched with various concentrations of NaCl (50, 100, 250, 350, 500mM) during three weeks at 16/8h photoperiod. Survival and bud formation were drastically reduced in all three species at high salt concentrations and biomass production in M. polymorpha declined almost exponentially as salt concentration increased. P. patens survived concentrations less than 350mM salt quite well. Chlorophyll content (-a, -b and total) in all examined species declined as salt concentration increased, however a modest rise in chlorophyll content in all three species was observed when the concentration of salt in the medium was 100mM. M. polymorpha did not tolerate well concentrations above 250mM according to all the parameters observed.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Programme and Abstracts: 18th Simposium Society of Plant Physiology of Serbia; 2009 May 25-27; Vršac, Serbia",
title = "Uticaj NaCl na morfogenezu i sadržaj hlorofila tri vrste briofita u kulturi in vitro, Influence of NaCl on morphogenesis and chlorophyll content of three bryophyte species in in vitro culture",
pages = "94",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6178"
}

Bogdanović, M., Sabovljević, A., Sabovljević, M.,& Grubišić, D.. (2009). Uticaj NaCl na morfogenezu i sadržaj hlorofila tri vrste briofita u kulturi in vitro. in Programme and Abstracts: 18th Simposium Society of Plant Physiology of Serbia; 2009 May 25-27; Vršac, Serbia
Belgrade: Serbian Plant Physiology Society., 94.
https://hdl.handle.net/21.15107/rcub_ibiss_6178

Bogdanović M, Sabovljević A, Sabovljević M, Grubišić D. Uticaj NaCl na morfogenezu i sadržaj hlorofila tri vrste briofita u kulturi in vitro. in Programme and Abstracts: 18th Simposium Society of Plant Physiology of Serbia; 2009 May 25-27; Vršac, Serbia. 2009;:94.
https://hdl.handle.net/21.15107/rcub_ibiss_6178 .

Bogdanović, Milica, Sabovljević, Aneta, Sabovljević, Marko, Grubišić, Dragoljub, "Uticaj NaCl na morfogenezu i sadržaj hlorofila tri vrste briofita u kulturi in vitro" in Programme and Abstracts: 18th Simposium Society of Plant Physiology of Serbia; 2009 May 25-27; Vršac, Serbia (2009):94,
https://hdl.handle.net/21.15107/rcub_ibiss_6178 .

TY  - JOUR
AU  - Subotić, Angelina
AU  - Jevremović, Slađana
AU  - Grubišić, Dragoljub
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1454
AB  - The purpose of this work was to acquire more information on the capacity of ill vitro grown Centaurium erythraea Gillib. normal and hairy root cultures to simultaneously regenerate adventitious buds and to evaluate the variations induced on regeneration response by treatments with six cytokinins. Explants from normal and hairy root cultures were cultured on half-strength MS medium (1/2 MS) with kinetin (KIN), N(6)-benzy[aminopurine (BA), 6-gamma,gamma-dimethylallylaminopurine (2IP), N-(2-chloro-4-pyridyl)-N'-phenylurea (CPPU), 1-Phenyl-3-(1,2,3-thiadiazol-5-yl)urea (TDZ) and 6-14-Hydroxy-3-methyl-but-2-enylamino]purine (ZEA), used alone in six different concentrations. The average number of adventitious buds per explant was promoted by all of cytokinin treatments. Urea-type cytokinins,TDZ and CPPU were more effective for the induction the morphogenesis of adventitious buds than adenine-type cytokinins. We found that the 1.0 mu M CPPU induced the largest number (25.6, 18.2, respectively) of adventitious buds in normal and hairy root culture. TDZ-supplemented media induced highest number of adventitious buds (24.2) from normal root explant, but from hairy root explant average number of buds is lower (20.5). Regenerated shoots were excised and placed on 1/2 MS medium without hormone. The rooted plantlets were successfully acclimatized in greenhouse conditions. (C) 2008 Elsevier B.V. All rights reserved.
T2  - Scientia Horticulturae
T1  - Influence of cytokinins on in vitro morphogenesis in root cultures of Centaurium erythraea-Valuable medicinal plant
IS  - 3
VL  - 120
EP  - 390
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1454
ER  - 

@article{
author = "Subotić, Angelina and Jevremović, Slađana and Grubišić, Dragoljub",
year = "2009",
abstract = "The purpose of this work was to acquire more information on the capacity of ill vitro grown Centaurium erythraea Gillib. normal and hairy root cultures to simultaneously regenerate adventitious buds and to evaluate the variations induced on regeneration response by treatments with six cytokinins. Explants from normal and hairy root cultures were cultured on half-strength MS medium (1/2 MS) with kinetin (KIN), N(6)-benzy[aminopurine (BA), 6-gamma,gamma-dimethylallylaminopurine (2IP), N-(2-chloro-4-pyridyl)-N'-phenylurea (CPPU), 1-Phenyl-3-(1,2,3-thiadiazol-5-yl)urea (TDZ) and 6-14-Hydroxy-3-methyl-but-2-enylamino]purine (ZEA), used alone in six different concentrations. The average number of adventitious buds per explant was promoted by all of cytokinin treatments. Urea-type cytokinins,TDZ and CPPU were more effective for the induction the morphogenesis of adventitious buds than adenine-type cytokinins. We found that the 1.0 mu M CPPU induced the largest number (25.6, 18.2, respectively) of adventitious buds in normal and hairy root culture. TDZ-supplemented media induced highest number of adventitious buds (24.2) from normal root explant, but from hairy root explant average number of buds is lower (20.5). Regenerated shoots were excised and placed on 1/2 MS medium without hormone. The rooted plantlets were successfully acclimatized in greenhouse conditions. (C) 2008 Elsevier B.V. All rights reserved.",
journal = "Scientia Horticulturae",
title = "Influence of cytokinins on in vitro morphogenesis in root cultures of Centaurium erythraea-Valuable medicinal plant",
number = "3",
volume = "120",
pages = "390",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1454"
}

Subotić, A., Jevremović, S.,& Grubišić, D.. (2009). Influence of cytokinins on in vitro morphogenesis in root cultures of Centaurium erythraea-Valuable medicinal plant. in Scientia Horticulturae, 120(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1454

Subotić A, Jevremović S, Grubišić D. Influence of cytokinins on in vitro morphogenesis in root cultures of Centaurium erythraea-Valuable medicinal plant. in Scientia Horticulturae. 2009;120(3):null-390.
https://hdl.handle.net/21.15107/rcub_ibiss_1454 .

Subotić, Angelina, Jevremović, Slađana, Grubišić, Dragoljub, "Influence of cytokinins on in vitro morphogenesis in root cultures of Centaurium erythraea-Valuable medicinal plant" in Scientia Horticulturae, 120, no. 3 (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1454 .

TY  - JOUR
AU  - Subotić, Angelina
AU  - Jevremović, Slađana
AU  - Trifunović-Momčilov, Milana
AU  - Petrić, Marija P
AU  - Milosević, Snezana M
AU  - Grubišić, Dragoljub
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1439
AB  - The effects of exogenous gibberellic acid (GA(3)) and paclobutrazol on induction of somatic embryos in wild type and hairy root culture of Centaurium erythraea Gillib. were investigated. Both compounds were incorperated into 1/2 MS medium at 6 concentrations (0.01, 0.03, 0.1, 0.3, 1.0 and 3.0 mu M). Wild type root and hairy root explants cultured in the presence of GA(3) at all tested concentrations under 16-h photoperiod or in the darkness decreased the number of somatic embryos that were produced. Paclobutrazol (0.3 mu M) induced the largest number (19.7, 16.5) of somatic embryos in wild type and hairy root cultures, respectively. Rooting of plants derived from somatic embryos as achieved on 1/2MS medium. These results indicate that paclobutrazol is beneficial for somatic embryo induction and formation in wild type and hairy root culture.
T2  - African Journal of Biotechnology
T1  - The influence of gibberellic acid and paclobutrazol on induction of somatic embryogenesis in wild type and hairy root cultures of Centaurium erythraea Gillib.
IS  - 14
VL  - 8
EP  - 3228
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1439
ER  - 

@article{
author = "Subotić, Angelina and Jevremović, Slađana and Trifunović-Momčilov, Milana and Petrić, Marija P and Milosević, Snezana M and Grubišić, Dragoljub",
year = "2009",
abstract = "The effects of exogenous gibberellic acid (GA(3)) and paclobutrazol on induction of somatic embryos in wild type and hairy root culture of Centaurium erythraea Gillib. were investigated. Both compounds were incorperated into 1/2 MS medium at 6 concentrations (0.01, 0.03, 0.1, 0.3, 1.0 and 3.0 mu M). Wild type root and hairy root explants cultured in the presence of GA(3) at all tested concentrations under 16-h photoperiod or in the darkness decreased the number of somatic embryos that were produced. Paclobutrazol (0.3 mu M) induced the largest number (19.7, 16.5) of somatic embryos in wild type and hairy root cultures, respectively. Rooting of plants derived from somatic embryos as achieved on 1/2MS medium. These results indicate that paclobutrazol is beneficial for somatic embryo induction and formation in wild type and hairy root culture.",
journal = "African Journal of Biotechnology",
title = "The influence of gibberellic acid and paclobutrazol on induction of somatic embryogenesis in wild type and hairy root cultures of Centaurium erythraea Gillib.",
number = "14",
volume = "8",
pages = "3228",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1439"
}

Subotić, A., Jevremović, S., Trifunović-Momčilov, M., Petrić, M. P., Milosević, S. M.,& Grubišić, D.. (2009). The influence of gibberellic acid and paclobutrazol on induction of somatic embryogenesis in wild type and hairy root cultures of Centaurium erythraea Gillib.. in African Journal of Biotechnology, 8(14).
https://hdl.handle.net/21.15107/rcub_ibiss_1439

Subotić A, Jevremović S, Trifunović-Momčilov M, Petrić MP, Milosević SM, Grubišić D. The influence of gibberellic acid and paclobutrazol on induction of somatic embryogenesis in wild type and hairy root cultures of Centaurium erythraea Gillib.. in African Journal of Biotechnology. 2009;8(14):null-3228.
https://hdl.handle.net/21.15107/rcub_ibiss_1439 .

Subotić, Angelina, Jevremović, Slađana, Trifunović-Momčilov, Milana, Petrić, Marija P, Milosević, Snezana M, Grubišić, Dragoljub, "The influence of gibberellic acid and paclobutrazol on induction of somatic embryogenesis in wild type and hairy root cultures of Centaurium erythraea Gillib." in African Journal of Biotechnology, 8, no. 14 (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1439 .

TY  - JOUR
AU  - Grbić-Ljaljević, Milica
AU  - Soković, Marina
AU  - Stupar, Miloš
AU  - Mišić, Danijela
AU  - Vukojević, Jelena
AU  - Grubišić, Dragoljub
AU  - Ristić, Mihailo
PY  - 2008
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/556
AB  - The chemical composition and antifungal activity of the essential oil of an endemic Serbian plant Nepeta rtanjensis Diklić & Milojević was studied. The essential oil was isolated from cultivated plants. Inhibition of mycelia growth of five micromycetes, two Alternaria species originally isolated from N. rtanjensis, Cladosporium cladosporoides, Trichoderma viride and Bipolaris spicifera, were tested using the agar dilution method. The essential oil of N. rtanjensis, the main component of which was 4aα,7α,7αβ-nepetalactone, showed strong antifungal activity against all the tested micromycetes. The minimum inhibitory concentration of N. rtanjensis essential oil ranged from 0.6 to 1.4 μg mL-1. The fungi most sensitive to the tested oil were Alternaria species, while Trichoderma viride was the most resistant.
AB  - U ovom radu prezentovan je hemijski sastav i antifungalna aktivnost etarskog ulja endemične biljke Nepeta rtanjensis Diklić & Milojević. Etarsko ulje je izolovano iz kultivisanih biljaka. Inhibicija micelijalnog rasta pet mikromiceta, dve vrste roda Alternaria, izolovane sa N. rtanjensis, Cladosporium cladosporioides, Trichoderma viride i Bipolaris spicifera, testirana je makrodilucionom metodom. Etarsko ulje N. rtanjensis, čija je glavna komponenta 4aα,7α,7aβ-nepetalakton pokazuje jaku antifungalnu aktivnost u odnosu na sve testirane mikromicete. Minimalna inhibitorna koncentracija (MIC) etaskog ulja bila je u rasponu od 0,6 μg mL-1 do 1,4 μg mL-1. Najveću osetljivost na testirano ulje pokazale su vrste roda Alternaria dok je Trichoderma viride bila najotpornija.
T2  - Journal of the Serbian Chemical Society
T1  - Antifungalna aktivnost etarskog ulja
T1  - Antifungal activity of Nepeta rtanjensis essential oil
IS  - 10
VL  - 73
SP  - 961
EP  - 965
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_556
ER  - 

@article{
author = "Grbić-Ljaljević, Milica and Soković, Marina and Stupar, Miloš and Mišić, Danijela and Vukojević, Jelena and Grubišić, Dragoljub and Ristić, Mihailo",
year = "2008, 2008",
abstract = "The chemical composition and antifungal activity of the essential oil of an endemic Serbian plant Nepeta rtanjensis Diklić & Milojević was studied. The essential oil was isolated from cultivated plants. Inhibition of mycelia growth of five micromycetes, two Alternaria species originally isolated from N. rtanjensis, Cladosporium cladosporoides, Trichoderma viride and Bipolaris spicifera, were tested using the agar dilution method. The essential oil of N. rtanjensis, the main component of which was 4aα,7α,7αβ-nepetalactone, showed strong antifungal activity against all the tested micromycetes. The minimum inhibitory concentration of N. rtanjensis essential oil ranged from 0.6 to 1.4 μg mL-1. The fungi most sensitive to the tested oil were Alternaria species, while Trichoderma viride was the most resistant., U ovom radu prezentovan je hemijski sastav i antifungalna aktivnost etarskog ulja endemične biljke Nepeta rtanjensis Diklić & Milojević. Etarsko ulje je izolovano iz kultivisanih biljaka. Inhibicija micelijalnog rasta pet mikromiceta, dve vrste roda Alternaria, izolovane sa N. rtanjensis, Cladosporium cladosporioides, Trichoderma viride i Bipolaris spicifera, testirana je makrodilucionom metodom. Etarsko ulje N. rtanjensis, čija je glavna komponenta 4aα,7α,7aβ-nepetalakton pokazuje jaku antifungalnu aktivnost u odnosu na sve testirane mikromicete. Minimalna inhibitorna koncentracija (MIC) etaskog ulja bila je u rasponu od 0,6 μg mL-1 do 1,4 μg mL-1. Najveću osetljivost na testirano ulje pokazale su vrste roda Alternaria dok je Trichoderma viride bila najotpornija.",
journal = "Journal of the Serbian Chemical Society",
title = "Antifungalna aktivnost etarskog ulja, Antifungal activity of Nepeta rtanjensis essential oil",
number = "10",
volume = "73",
pages = "961-965",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_556"
}

Grbić-Ljaljević, M., Soković, M., Stupar, M., Mišić, D., Vukojević, J., Grubišić, D.,& Ristić, M.. (2008). Antifungalna aktivnost etarskog ulja. in Journal of the Serbian Chemical Society, 73(10), 961-965.
https://hdl.handle.net/21.15107/rcub_ibiss_556

Grbić-Ljaljević M, Soković M, Stupar M, Mišić D, Vukojević J, Grubišić D, Ristić M. Antifungalna aktivnost etarskog ulja. in Journal of the Serbian Chemical Society. 2008;73(10):961-965.
https://hdl.handle.net/21.15107/rcub_ibiss_556 .

Grbić-Ljaljević, Milica, Soković, Marina, Stupar, Miloš, Mišić, Danijela, Vukojević, Jelena, Grubišić, Dragoljub, Ristić, Mihailo, "Antifungalna aktivnost etarskog ulja" in Journal of the Serbian Chemical Society, 73, no. 10 (2008):961-965,
https://hdl.handle.net/21.15107/rcub_ibiss_556 .

TY  - JOUR
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana
AU  - Grubišić, Dragoljub
AU  - Stojković, Dejan
AU  - Ristić, Mihailo S.
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1526
AB  - Essential oils were isolated from the aerial pails during the flowering and vegetative phases, roots and seeds of the plant Portenschlagiella ramosissima and their antimicrobial activities against bacteria (Salmonella enteritidis, Escherichia coli, Micrococcus flavus, Bacillus subtilis, Staphylococcus aureus) and micromycetes (Aspergillus flavus, A. niger, A. versicolor, A. ochrachloron, Penicillium funiculosum, Trichoderma viride) were tested. Among tested bacterial and fungal species were plant pathogens, food poisoning species, animal and human pathogens. The main component in all oils samples was myristicin (61.1-91.5%). Antimicrobial activity of oils was tested using the micro-dilution and disc-diffusion methods. All of the oils tested showed activity.
T2  - Journal of Essential Oil Research
T1  - Antimicrobial activity of essential oils isolated from different parts of endemic plan Portenschlagiella ramosissima Tutin
IS  - 4
VL  - 20
EP  - 372
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1526
ER  - 

@article{
author = "Soković, Marina and Glamočlija, Jasmina and Ćirić, Ana and Grubišić, Dragoljub and Stojković, Dejan and Ristić, Mihailo S.",
year = "2008",
abstract = "Essential oils were isolated from the aerial pails during the flowering and vegetative phases, roots and seeds of the plant Portenschlagiella ramosissima and their antimicrobial activities against bacteria (Salmonella enteritidis, Escherichia coli, Micrococcus flavus, Bacillus subtilis, Staphylococcus aureus) and micromycetes (Aspergillus flavus, A. niger, A. versicolor, A. ochrachloron, Penicillium funiculosum, Trichoderma viride) were tested. Among tested bacterial and fungal species were plant pathogens, food poisoning species, animal and human pathogens. The main component in all oils samples was myristicin (61.1-91.5%). Antimicrobial activity of oils was tested using the micro-dilution and disc-diffusion methods. All of the oils tested showed activity.",
journal = "Journal of Essential Oil Research",
title = "Antimicrobial activity of essential oils isolated from different parts of endemic plan Portenschlagiella ramosissima Tutin",
number = "4",
volume = "20",
pages = "372",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1526"
}

Soković, M., Glamočlija, J., Ćirić, A., Grubišić, D., Stojković, D.,& Ristić, M. S.. (2008). Antimicrobial activity of essential oils isolated from different parts of endemic plan Portenschlagiella ramosissima Tutin. in Journal of Essential Oil Research, 20(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1526

Soković M, Glamočlija J, Ćirić A, Grubišić D, Stojković D, Ristić MS. Antimicrobial activity of essential oils isolated from different parts of endemic plan Portenschlagiella ramosissima Tutin. in Journal of Essential Oil Research. 2008;20(4):null-372.
https://hdl.handle.net/21.15107/rcub_ibiss_1526 .

Soković, Marina, Glamočlija, Jasmina, Ćirić, Ana, Grubišić, Dragoljub, Stojković, Dejan, Ristić, Mihailo S., "Antimicrobial activity of essential oils isolated from different parts of endemic plan Portenschlagiella ramosissima Tutin" in Journal of Essential Oil Research, 20, no. 4 (2008),
https://hdl.handle.net/21.15107/rcub_ibiss_1526 .

TY  - JOUR
AU  - Todorović, Slađana
AU  - Giba, Zlatko
AU  - Bacić, Goran G
AU  - Nikolić, Miroslav
AU  - Grubišić, Dragoljub
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1498
AB  - The effect of high Mn2+ content on Centaurium pulchellum seed germination has been investigated. Seeds containing extremely high Mn2+ content were produced by culturing single-node flowering explants for 2 months in the MS-media, supplemented with Mn in concentrations ranging from 1 to 10,000 mu M. Although the seeds displayed the capacity to accumulate high amount of Mn, their germination was undisturbed. EPR spectroscopy was used to measure the ratio of free (aqueous) Mn to bound Mn and it was found that over 97% of total Mn was in the bound form. With elevating the external Mn supply, seed Mn concentration also increased, but the proportion of free Mn2+ fraction decreased from 3% in the control (1 mu M Mn) to 0.35% and 0.15% in high Mn supply (1000 mu M and 10,000 mu M, respectively). These results suggest that an elevation of internal Mn concentration in seeds is associated with increased Mn binding pools, hence Mn remains bound during germination. Consequently, the action of potentially harmful Mn2+ ions, which may generate ROS and affect seed viability, is alleviated. (C) 2008 Elsevier B.V. All rights reserved.
T2  - Environmental and Experimental Botany
T1  - High seed Mn content does not affect germination of in vitro produced Centaurium pulchellum seeds
IS  - 3
VL  - 64
EP  - 324
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1498
ER  - 

@article{
author = "Todorović, Slađana and Giba, Zlatko and Bacić, Goran G and Nikolić, Miroslav and Grubišić, Dragoljub",
year = "2008",
abstract = "The effect of high Mn2+ content on Centaurium pulchellum seed germination has been investigated. Seeds containing extremely high Mn2+ content were produced by culturing single-node flowering explants for 2 months in the MS-media, supplemented with Mn in concentrations ranging from 1 to 10,000 mu M. Although the seeds displayed the capacity to accumulate high amount of Mn, their germination was undisturbed. EPR spectroscopy was used to measure the ratio of free (aqueous) Mn to bound Mn and it was found that over 97% of total Mn was in the bound form. With elevating the external Mn supply, seed Mn concentration also increased, but the proportion of free Mn2+ fraction decreased from 3% in the control (1 mu M Mn) to 0.35% and 0.15% in high Mn supply (1000 mu M and 10,000 mu M, respectively). These results suggest that an elevation of internal Mn concentration in seeds is associated with increased Mn binding pools, hence Mn remains bound during germination. Consequently, the action of potentially harmful Mn2+ ions, which may generate ROS and affect seed viability, is alleviated. (C) 2008 Elsevier B.V. All rights reserved.",
journal = "Environmental and Experimental Botany",
title = "High seed Mn content does not affect germination of in vitro produced Centaurium pulchellum seeds",
number = "3",
volume = "64",
pages = "324",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1498"
}

Todorović, S., Giba, Z., Bacić, G. G., Nikolić, M.,& Grubišić, D.. (2008). High seed Mn content does not affect germination of in vitro produced Centaurium pulchellum seeds. in Environmental and Experimental Botany, 64(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1498

Todorović S, Giba Z, Bacić GG, Nikolić M, Grubišić D. High seed Mn content does not affect germination of in vitro produced Centaurium pulchellum seeds. in Environmental and Experimental Botany. 2008;64(3):null-324.
https://hdl.handle.net/21.15107/rcub_ibiss_1498 .

Todorović, Slađana, Giba, Zlatko, Bacić, Goran G, Nikolić, Miroslav, Grubišić, Dragoljub, "High seed Mn content does not affect germination of in vitro produced Centaurium pulchellum seeds" in Environmental and Experimental Botany, 64, no. 3 (2008),
https://hdl.handle.net/21.15107/rcub_ibiss_1498 .

TY  - JOUR
AU  - Ljaljević-Grbić, Milica V.
AU  - Soković, Marina
AU  - Vukojević, Jelena B.
AU  - Grubišić, Dragoljub
AU  - Ristić, Mihailo
PY  - 2007
PY  - 2007
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/537
AB  - The antifungal activity of Nepeta rtanjensis Diklić et Milojević essential oil on mycelia growth has been performed by macrodilution method. The most efficient impact of N. rtanjensis essential oil on mycelia growth in vitro was found in Alternaria species with the same value of minimal inhibitory quantity (MIQ) of 0.6 μl/ml. Bipolaris spicifera and Cladosporium cladosporoioides had MIQ values of 1.0 μl/ml whereas Trichoderma viride with MIQ value of 1.6 μl/ml showed the most efficient defense against the essential oil examined. The values of minimal fungicidal quantity (MFQ) in Alternaria sp. 2, B. spicifera and C. cladosporioides match the MIQ values whereas MFQ values in Alternaria sp. 1 is 0.8 μl/ml, and in T. viride 1.8 μl/ml.
AB  - Makrodilucionom metodom testiran je uticaj etarskog ulja endemične biljke Nepeta rtanjensis (Lamiaceae) na micelijalni rast mikromiceta: Alternaria sp. 1, Alternaria sp. 2, Bipolaris spicifera, Cladosporium cladosporioides i Trichoderma viride. Hemijska analiza etarskog ulja N. rtanjensis pokazala je apsolutnu dominaciju 4aα, 7α, 7aβ nepetalaktona (79.89%). Najefikasniji uticaj na rast micelije in vitro zabeležen je kod vrsta roda Alternaria sa istom vrednošću minimalne inhibitorne količine (MIK) od 0.6 μl/ml. Bipolaris spicifera i Cladosporium cladosporioides su imali MIK 1.0 μl/ml, dok je Trichoderma viride, sa vrednošću MIK od 1.6 μl/ml, pokazala najveću otpornost na dejstvo ispitivanog ulja. Vrednosti minimalne fungicidne količine (MFK) se kod Alternaria sp. 2 (0.6 μl/ml), B. spicifera (1.0 μl/ml) i C. cladosporioides (1.0 μl/ml) poklapaju sa vrednostima MIK, dok je kod Alternaria sp. 1 MFK 0.8 μl/ml, a kod T. viride 1.8 μl/ml. Etarsko ulje N. rtanjensis pokazalo je jako antifungalno dejstvo na rast micelije testiranih mikromiceta.
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Uticaj etarskog ulja Nepeta rtanjensis na rast micelije test mikromiceta
T1  - The effect of Nepeta rtanjensis essential oil on test micromycetes mycelia growth
IS  - 113
VL  - null
SP  - 173
EP  - 177
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_537
ER  - 

@article{
author = "Ljaljević-Grbić, Milica V. and Soković, Marina and Vukojević, Jelena B. and Grubišić, Dragoljub and Ristić, Mihailo",
year = "2007, 2007",
abstract = "The antifungal activity of Nepeta rtanjensis Diklić et Milojević essential oil on mycelia growth has been performed by macrodilution method. The most efficient impact of N. rtanjensis essential oil on mycelia growth in vitro was found in Alternaria species with the same value of minimal inhibitory quantity (MIQ) of 0.6 μl/ml. Bipolaris spicifera and Cladosporium cladosporoioides had MIQ values of 1.0 μl/ml whereas Trichoderma viride with MIQ value of 1.6 μl/ml showed the most efficient defense against the essential oil examined. The values of minimal fungicidal quantity (MFQ) in Alternaria sp. 2, B. spicifera and C. cladosporioides match the MIQ values whereas MFQ values in Alternaria sp. 1 is 0.8 μl/ml, and in T. viride 1.8 μl/ml., Makrodilucionom metodom testiran je uticaj etarskog ulja endemične biljke Nepeta rtanjensis (Lamiaceae) na micelijalni rast mikromiceta: Alternaria sp. 1, Alternaria sp. 2, Bipolaris spicifera, Cladosporium cladosporioides i Trichoderma viride. Hemijska analiza etarskog ulja N. rtanjensis pokazala je apsolutnu dominaciju 4aα, 7α, 7aβ nepetalaktona (79.89%). Najefikasniji uticaj na rast micelije in vitro zabeležen je kod vrsta roda Alternaria sa istom vrednošću minimalne inhibitorne količine (MIK) od 0.6 μl/ml. Bipolaris spicifera i Cladosporium cladosporioides su imali MIK 1.0 μl/ml, dok je Trichoderma viride, sa vrednošću MIK od 1.6 μl/ml, pokazala najveću otpornost na dejstvo ispitivanog ulja. Vrednosti minimalne fungicidne količine (MFK) se kod Alternaria sp. 2 (0.6 μl/ml), B. spicifera (1.0 μl/ml) i C. cladosporioides (1.0 μl/ml) poklapaju sa vrednostima MIK, dok je kod Alternaria sp. 1 MFK 0.8 μl/ml, a kod T. viride 1.8 μl/ml. Etarsko ulje N. rtanjensis pokazalo je jako antifungalno dejstvo na rast micelije testiranih mikromiceta.",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Uticaj etarskog ulja Nepeta rtanjensis na rast micelije test mikromiceta, The effect of Nepeta rtanjensis essential oil on test micromycetes mycelia growth",
number = "113",
volume = "null",
pages = "173-177",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_537"
}

Ljaljević-Grbić, M. V., Soković, M., Vukojević, J. B., Grubišić, D.,& Ristić, M.. (2007). Uticaj etarskog ulja Nepeta rtanjensis na rast micelije test mikromiceta. in Zbornik Matice srpske za prirodne nauke, null(113), 173-177.
https://hdl.handle.net/21.15107/rcub_ibiss_537

Ljaljević-Grbić MV, Soković M, Vukojević JB, Grubišić D, Ristić M. Uticaj etarskog ulja Nepeta rtanjensis na rast micelije test mikromiceta. in Zbornik Matice srpske za prirodne nauke. 2007;null(113):173-177.
https://hdl.handle.net/21.15107/rcub_ibiss_537 .

Ljaljević-Grbić, Milica V., Soković, Marina, Vukojević, Jelena B., Grubišić, Dragoljub, Ristić, Mihailo, "Uticaj etarskog ulja Nepeta rtanjensis na rast micelije test mikromiceta" in Zbornik Matice srpske za prirodne nauke, null, no. 113 (2007):173-177,
https://hdl.handle.net/21.15107/rcub_ibiss_537 .

TY  - JOUR
AU  - Todorović, Slađana
AU  - Živković, Suzana
AU  - Giba, Zlatko
AU  - Mišić, Danijela
AU  - Grubišić, Dragoljub
PY  - 2007
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1569
AB  - Nepeta rtanjensis is an endemic plant species that was first recorded in 1974 in the eastern part of Serbia. Because of the small number of discovered specimens and low seed viability, it has been difficult to determine the seed germination characteristics of this species. Using in vitro culture techniques, we obtained sufficient plants for successful reintroduction. In the first year approximately 500 000 seeds were collected and subjected to various seed germination tests. The seeds were positive photoblastic and germination was under the control of the phytochrome pigment system. The requirement for light could be substituted by gibberellins. The seeds were nitrate insensitive, but responded to treatment with liquid smoke.
T2  - Plant Species Biology
T1  - Basic seed germination characteristics of the endemic species Nepeta rtanjensis (Lamiaceae)
IS  - 3
VL  - 22
EP  - 210
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1569
ER  - 

@article{
author = "Todorović, Slađana and Živković, Suzana and Giba, Zlatko and Mišić, Danijela and Grubišić, Dragoljub",
year = "2007",
abstract = "Nepeta rtanjensis is an endemic plant species that was first recorded in 1974 in the eastern part of Serbia. Because of the small number of discovered specimens and low seed viability, it has been difficult to determine the seed germination characteristics of this species. Using in vitro culture techniques, we obtained sufficient plants for successful reintroduction. In the first year approximately 500 000 seeds were collected and subjected to various seed germination tests. The seeds were positive photoblastic and germination was under the control of the phytochrome pigment system. The requirement for light could be substituted by gibberellins. The seeds were nitrate insensitive, but responded to treatment with liquid smoke.",
journal = "Plant Species Biology",
title = "Basic seed germination characteristics of the endemic species Nepeta rtanjensis (Lamiaceae)",
number = "3",
volume = "22",
pages = "210",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1569"
}

Todorović, S., Živković, S., Giba, Z., Mišić, D.,& Grubišić, D.. (2007). Basic seed germination characteristics of the endemic species Nepeta rtanjensis (Lamiaceae). in Plant Species Biology, 22(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1569

Todorović S, Živković S, Giba Z, Mišić D, Grubišić D. Basic seed germination characteristics of the endemic species Nepeta rtanjensis (Lamiaceae). in Plant Species Biology. 2007;22(3):null-210.
https://hdl.handle.net/21.15107/rcub_ibiss_1569 .

Todorović, Slađana, Živković, Suzana, Giba, Zlatko, Mišić, Danijela, Grubišić, Dragoljub, "Basic seed germination characteristics of the endemic species Nepeta rtanjensis (Lamiaceae)" in Plant Species Biology, 22, no. 3 (2007),
https://hdl.handle.net/21.15107/rcub_ibiss_1569 .

TY  - GEN
AU  - Grubišić, Dragoljub
AU  - Giba, Zlatko
PY  - 2007
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1608
T2  - Seed Science Research
T1  - Radomir M Konjevic (1 August 1946-22 July 2006)
IS  - 1
VL  - 17
EP  - 70
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1608
ER  - 

@misc{
author = "Grubišić, Dragoljub and Giba, Zlatko",
year = "2007",
journal = "Seed Science Research",
title = "Radomir M Konjevic (1 August 1946-22 July 2006)",
number = "1",
volume = "17",
pages = "70",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1608"
}

Grubišić, D.,& Giba, Z.. (2007). Radomir M Konjevic (1 August 1946-22 July 2006). in Seed Science Research, 17(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1608

Grubišić D, Giba Z. Radomir M Konjevic (1 August 1946-22 July 2006). in Seed Science Research. 2007;17(1):null-70.
https://hdl.handle.net/21.15107/rcub_ibiss_1608 .

Grubišić, Dragoljub, Giba, Zlatko, "Radomir M Konjevic (1 August 1946-22 July 2006)" in Seed Science Research, 17, no. 1 (2007),
https://hdl.handle.net/21.15107/rcub_ibiss_1608 .

TY  - JOUR
AU  - Subotić, Angelina
AU  - Grubišić, Dragoljub
PY  - 2007
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1578
AB  - Direct somatic embryogenesis and adventitious shoot formation were successfully achieved from root explants of Centaurium erythrea Gillib. cultured on Murashige and Skoog medium with half-strength macronutrients, full-strength micronutrients and vitamins, 3 % sucrose, 0.7 % agar, 100 mg dm(-3) myo-inositol and without growth regulators. Histological studies revealed that somatic embryos were formed directly from epidermal cells and adventitious buds were developed from meristematic cells in root cortex tissues. Somatic embryos as well as adventitious shoots developed into whole plantlets.
T2  - Biologia Plantarum
T1  - Histological analysis of somatic embryogenesis and adventitious shoot formation from root explants of Centaurium erythreae Gillib
IS  - 3
VL  - 51
EP  - 516
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1578
ER  - 

@article{
author = "Subotić, Angelina and Grubišić, Dragoljub",
year = "2007",
abstract = "Direct somatic embryogenesis and adventitious shoot formation were successfully achieved from root explants of Centaurium erythrea Gillib. cultured on Murashige and Skoog medium with half-strength macronutrients, full-strength micronutrients and vitamins, 3 % sucrose, 0.7 % agar, 100 mg dm(-3) myo-inositol and without growth regulators. Histological studies revealed that somatic embryos were formed directly from epidermal cells and adventitious buds were developed from meristematic cells in root cortex tissues. Somatic embryos as well as adventitious shoots developed into whole plantlets.",
journal = "Biologia Plantarum",
title = "Histological analysis of somatic embryogenesis and adventitious shoot formation from root explants of Centaurium erythreae Gillib",
number = "3",
volume = "51",
pages = "516",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1578"
}

Subotić, A.,& Grubišić, D.. (2007). Histological analysis of somatic embryogenesis and adventitious shoot formation from root explants of Centaurium erythreae Gillib. in Biologia Plantarum, 51(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1578

Subotić A, Grubišić D. Histological analysis of somatic embryogenesis and adventitious shoot formation from root explants of Centaurium erythreae Gillib. in Biologia Plantarum. 2007;51(3):null-516.
https://hdl.handle.net/21.15107/rcub_ibiss_1578 .

Subotić, Angelina, Grubišić, Dragoljub, "Histological analysis of somatic embryogenesis and adventitious shoot formation from root explants of Centaurium erythreae Gillib" in Biologia Plantarum, 51, no. 3 (2007),
https://hdl.handle.net/21.15107/rcub_ibiss_1578 .

TY  - JOUR
AU  - Rosić, Nedeljka
AU  - Momčilović, Ivana
AU  - Kovacević, Nada N
AU  - Grubišić, Dragoljub
PY  - 2006
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1623
AB  - Hairy roots of Rhamnus fallax Boiss. were induced using Agrobacterium rhizogenes strain A4M70GUS. The culture established on Woody plant media (WPM) showed a typical hairy root phenotype: rapid growth, reduced apical dominance and root plagiotropism. Seven clones of R. fallax were selected on the basis of their differences in colour and the root branching. The growth of hairy root culture, measured through gain in fresh mass, was done under 16-h photoperiod or in the dark. An increase in anthraquinone (AQ) content was obtained in clones with yellow and less branched roots, like clone 1 [16.43 mg g(-1)(d.m.)] and clone 7 [14.21 mg g(-1)(d.m.)], compared with other analysed transformed and non-transformed tissue. This study presents the first report of successful transformation of any species from family Rhamnaceae by A. rhizogenes and analysis of AQ production in transformed tissue.
T2  - Biologia Plantarum
T1  - Genetic transformation of Rhamnus fallax and hairy roots as a source of anthraquinones
IS  - 4
VL  - 50
EP  - 518
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1623
ER  - 

@article{
author = "Rosić, Nedeljka and Momčilović, Ivana and Kovacević, Nada N and Grubišić, Dragoljub",
year = "2006",
abstract = "Hairy roots of Rhamnus fallax Boiss. were induced using Agrobacterium rhizogenes strain A4M70GUS. The culture established on Woody plant media (WPM) showed a typical hairy root phenotype: rapid growth, reduced apical dominance and root plagiotropism. Seven clones of R. fallax were selected on the basis of their differences in colour and the root branching. The growth of hairy root culture, measured through gain in fresh mass, was done under 16-h photoperiod or in the dark. An increase in anthraquinone (AQ) content was obtained in clones with yellow and less branched roots, like clone 1 [16.43 mg g(-1)(d.m.)] and clone 7 [14.21 mg g(-1)(d.m.)], compared with other analysed transformed and non-transformed tissue. This study presents the first report of successful transformation of any species from family Rhamnaceae by A. rhizogenes and analysis of AQ production in transformed tissue.",
journal = "Biologia Plantarum",
title = "Genetic transformation of Rhamnus fallax and hairy roots as a source of anthraquinones",
number = "4",
volume = "50",
pages = "518",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1623"
}

Rosić, N., Momčilović, I., Kovacević, N. N.,& Grubišić, D.. (2006). Genetic transformation of Rhamnus fallax and hairy roots as a source of anthraquinones. in Biologia Plantarum, 50(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1623

Rosić N, Momčilović I, Kovacević NN, Grubišić D. Genetic transformation of Rhamnus fallax and hairy roots as a source of anthraquinones. in Biologia Plantarum. 2006;50(4):null-518.
https://hdl.handle.net/21.15107/rcub_ibiss_1623 .

Rosić, Nedeljka, Momčilović, Ivana, Kovacević, Nada N, Grubišić, Dragoljub, "Genetic transformation of Rhamnus fallax and hairy roots as a source of anthraquinones" in Biologia Plantarum, 50, no. 4 (2006),
https://hdl.handle.net/21.15107/rcub_ibiss_1623 .

TY  - JOUR
AU  - Puac, Nevena
AU  - Petrović, Zoran Lj
AU  - Malović, Gordana N
AU  - Đorđević, Antonije R
AU  - Živković, Suzana
AU  - Giba, Zlatko
AU  - Grubišić, Dragoljub
PY  - 2006
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1638
AB  - In this paper we present voltage-current-power characteristics of a plasma needle operating in the flow of helium at atmospheric pressure. In addition, we show some examples of how such a plasma affects plant tissues. In the characterization of the plasma needle, current and voltage waveforms were recorded by two derivative probes. These two probes are similar to the probes previously used by Puac et al for measuring transmitted power in low pressure CCP rf discharge. The instantaneous power was calculated from current and voltage waveforms and U-I characteristics of the discharge were determined. Regimes of operation with and without the grounding ring at the tip of the needle were considered. We have chosen two model systems to study the effect of the plasma needle on plant cells and tissues: sweet fern gametophyte (prothallus) and calli produced in vitro. Since the prothallus consists of a single layer of cells, the cytological effects could be easily examined. In addition, calli and prothallus are easy to manipulate and in vitro culture provides a possibility to work under constant and controlled conditions.
T2  - Journal of Physics D-Applied Physics
T1  - Measurements of voltage-current characteristics of a plasma needle and its effect on plant cells
IS  - 16
VL  - 39
EP  - 3519
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1638
ER  - 

@article{
author = "Puac, Nevena and Petrović, Zoran Lj and Malović, Gordana N and Đorđević, Antonije R and Živković, Suzana and Giba, Zlatko and Grubišić, Dragoljub",
year = "2006",
abstract = "In this paper we present voltage-current-power characteristics of a plasma needle operating in the flow of helium at atmospheric pressure. In addition, we show some examples of how such a plasma affects plant tissues. In the characterization of the plasma needle, current and voltage waveforms were recorded by two derivative probes. These two probes are similar to the probes previously used by Puac et al for measuring transmitted power in low pressure CCP rf discharge. The instantaneous power was calculated from current and voltage waveforms and U-I characteristics of the discharge were determined. Regimes of operation with and without the grounding ring at the tip of the needle were considered. We have chosen two model systems to study the effect of the plasma needle on plant cells and tissues: sweet fern gametophyte (prothallus) and calli produced in vitro. Since the prothallus consists of a single layer of cells, the cytological effects could be easily examined. In addition, calli and prothallus are easy to manipulate and in vitro culture provides a possibility to work under constant and controlled conditions.",
journal = "Journal of Physics D-Applied Physics",
title = "Measurements of voltage-current characteristics of a plasma needle and its effect on plant cells",
number = "16",
volume = "39",
pages = "3519",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1638"
}

Puac, N., Petrović, Z. L., Malović, G. N., Đorđević, A. R., Živković, S., Giba, Z.,& Grubišić, D.. (2006). Measurements of voltage-current characteristics of a plasma needle and its effect on plant cells. in Journal of Physics D-Applied Physics, 39(16).
https://hdl.handle.net/21.15107/rcub_ibiss_1638

Puac N, Petrović ZL, Malović GN, Đorđević AR, Živković S, Giba Z, Grubišić D. Measurements of voltage-current characteristics of a plasma needle and its effect on plant cells. in Journal of Physics D-Applied Physics. 2006;39(16):null-3519.
https://hdl.handle.net/21.15107/rcub_ibiss_1638 .

Puac, Nevena, Petrović, Zoran Lj, Malović, Gordana N, Đorđević, Antonije R, Živković, Suzana, Giba, Zlatko, Grubišić, Dragoljub, "Measurements of voltage-current characteristics of a plasma needle and its effect on plant cells" in Journal of Physics D-Applied Physics, 39, no. 16 (2006),
https://hdl.handle.net/21.15107/rcub_ibiss_1638 .

TY  - JOUR
AU  - Todorović, Slađana
AU  - Grubišić, Dragoljub
AU  - Giba, Zlatko
AU  - Mišić, Danijela
AU  - Konjević, Radomir M
PY  - 2006
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1626
AB  - The effect of sucrose on fruiting, seed production, and seed germination of lesser centaury [Centaurium pulchellum (Sw.) Druce] was examined using explants of flowers and flower buds. Sucrose concentrations in the culture medium ranged from 0.003 to 0.3 M. It has been shown that the number of auxiliary buds, capsules dimension, number of viable seeds per capsule and seed dimensions increased with the increase of sucrose concentrations. The highest values were recorded at sucrose concentrations higher than 0.03 M, except for seeds size, which were larger at sucrose concentration ranging from 0.003 to 0.1 M. The germination of in vitro produced seeds was affected by previous culture history: a higher germination percentage was obtained in seeds that were raised from explants originally grown on medium with sucrose concentrations higher than 0.003 M.
T2  - Biologia Plantarum
T1  - Sucrose effects on in vitro fruiting and seed production of Centaurium pulchellum
IS  - 4
VL  - 50
EP  - 774
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1626
ER  - 

@article{
author = "Todorović, Slađana and Grubišić, Dragoljub and Giba, Zlatko and Mišić, Danijela and Konjević, Radomir M",
year = "2006",
abstract = "The effect of sucrose on fruiting, seed production, and seed germination of lesser centaury [Centaurium pulchellum (Sw.) Druce] was examined using explants of flowers and flower buds. Sucrose concentrations in the culture medium ranged from 0.003 to 0.3 M. It has been shown that the number of auxiliary buds, capsules dimension, number of viable seeds per capsule and seed dimensions increased with the increase of sucrose concentrations. The highest values were recorded at sucrose concentrations higher than 0.03 M, except for seeds size, which were larger at sucrose concentration ranging from 0.003 to 0.1 M. The germination of in vitro produced seeds was affected by previous culture history: a higher germination percentage was obtained in seeds that were raised from explants originally grown on medium with sucrose concentrations higher than 0.003 M.",
journal = "Biologia Plantarum",
title = "Sucrose effects on in vitro fruiting and seed production of Centaurium pulchellum",
number = "4",
volume = "50",
pages = "774",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1626"
}

Todorović, S., Grubišić, D., Giba, Z., Mišić, D.,& Konjević, R. M.. (2006). Sucrose effects on in vitro fruiting and seed production of Centaurium pulchellum. in Biologia Plantarum, 50(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1626

Todorović S, Grubišić D, Giba Z, Mišić D, Konjević RM. Sucrose effects on in vitro fruiting and seed production of Centaurium pulchellum. in Biologia Plantarum. 2006;50(4):null-774.
https://hdl.handle.net/21.15107/rcub_ibiss_1626 .

Todorović, Slađana, Grubišić, Dragoljub, Giba, Zlatko, Mišić, Danijela, Konjević, Radomir M, "Sucrose effects on in vitro fruiting and seed production of Centaurium pulchellum" in Biologia Plantarum, 50, no. 4 (2006),
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