TY  - CONF
AU  - Añibarro-Ortega, Mikel
AU  - Pinela, José
AU  - Ćirić, Ana
AU  - Rocha, Filomena
AU  - Barata, Ana Maria
AU  - Soković, Marina
AU  - Carvalho, Ana Maria
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4374
AB  - Table tomato (Solanum lycopersicum L.) is highly consumed worldwide and its annual production generates 
a considerable amount of plant by-products [1]. Although tomatoes are well-known for their composition 
in nutrients and bioactive compounds [2,3], little information is available in literature regarding tomato 
leaves, probably because these by-products have no commercial value. Therefore, considering the enormous
production of this biomass and the current trends of circularity and sustainable development [3], this study 
was carried out to evaluate the polyphenolic composition and bioactive properties of tomato leaves resulting 
from the regeneration of table tomato landraces conserved ex-situ in the Portuguese Genebank (BPGV), in 
Braga, Portugal. The tomato plant material resulting from pruning performed after the flowering season was
lyophilized and ground to a fine powder, used to prepare hydroethanolic extracts [4]. These extracts were 
characterized for their composition in polyphenols (by HPLC-DAD-ESI/MSn
) and used to evaluate the 
antioxidant (by DPPH•
scavenging capacity and reduction power), antihemolytic (using sheep erythrocytes 
and AAPH as a free radical generator), and antimicrobial (tested against food-borne bacterial and fungal 
strains by the serial microdilution and p-iodonitrotetrazolium violet colorimetric methods) activities [4].
The chromatographic analysis allowed identifying phenolic acids and flavonoids, with prevalence of 
quercetin-3-O-rutinoside (rutin). The leaf extracts showed antioxidant activity, with EC50 values lower than 
those previously reported for the respective tomato fruits, thus translating a higher activity [2]. The extracts 
were also effective in some extent in protecting the erythrocytes from the oxidative hemolysis caused the 
thermal decomposition of the free-radical initiator AAPH. Despite their low activity against the tested 
microfungi, some extracts had ability to inhibit and kill some bacteria (including Salmonella typhimurium, 
Listeria monocytogenes, Bacillus cereus, and Enterobacter cloacae) more effectively than the antibiotic 
ampicillin. It was concluded that table tomato crop by-products can be used to produce rutin-rich extracts 
with antioxidant and antibacterial activities for possible use in the agri-food sector as natural preservatives.
PB  - Bragança, Portugal: Instituto Politécnico de Bragança (IPB)
C3  - 1st Natural products application: Health, Cosmetic and Food: book of abstracts
T1  - Table tomato leaves are a sustainable source of rutin and display antioxidant, antihemolytic and antimicrobial activity
SP  - 73
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4374
ER  - 

@conference{
author = "Añibarro-Ortega, Mikel and Pinela, José and Ćirić, Ana and Rocha, Filomena and Barata, Ana Maria and Soković, Marina and Carvalho, Ana Maria and Ferreira, Isabel C. F. R. and Barros, Lillian",
year = "2021",
abstract = "Table tomato (Solanum lycopersicum L.) is highly consumed worldwide and its annual production generates 
a considerable amount of plant by-products [1]. Although tomatoes are well-known for their composition 
in nutrients and bioactive compounds [2,3], little information is available in literature regarding tomato 
leaves, probably because these by-products have no commercial value. Therefore, considering the enormous
production of this biomass and the current trends of circularity and sustainable development [3], this study 
was carried out to evaluate the polyphenolic composition and bioactive properties of tomato leaves resulting 
from the regeneration of table tomato landraces conserved ex-situ in the Portuguese Genebank (BPGV), in 
Braga, Portugal. The tomato plant material resulting from pruning performed after the flowering season was
lyophilized and ground to a fine powder, used to prepare hydroethanolic extracts [4]. These extracts were 
characterized for their composition in polyphenols (by HPLC-DAD-ESI/MSn
) and used to evaluate the 
antioxidant (by DPPH•
scavenging capacity and reduction power), antihemolytic (using sheep erythrocytes 
and AAPH as a free radical generator), and antimicrobial (tested against food-borne bacterial and fungal 
strains by the serial microdilution and p-iodonitrotetrazolium violet colorimetric methods) activities [4].
The chromatographic analysis allowed identifying phenolic acids and flavonoids, with prevalence of 
quercetin-3-O-rutinoside (rutin). The leaf extracts showed antioxidant activity, with EC50 values lower than 
those previously reported for the respective tomato fruits, thus translating a higher activity [2]. The extracts 
were also effective in some extent in protecting the erythrocytes from the oxidative hemolysis caused the 
thermal decomposition of the free-radical initiator AAPH. Despite their low activity against the tested 
microfungi, some extracts had ability to inhibit and kill some bacteria (including Salmonella typhimurium, 
Listeria monocytogenes, Bacillus cereus, and Enterobacter cloacae) more effectively than the antibiotic 
ampicillin. It was concluded that table tomato crop by-products can be used to produce rutin-rich extracts 
with antioxidant and antibacterial activities for possible use in the agri-food sector as natural preservatives.",
publisher = "Bragança, Portugal: Instituto Politécnico de Bragança (IPB)",
journal = "1st Natural products application: Health, Cosmetic and Food: book of abstracts",
title = "Table tomato leaves are a sustainable source of rutin and display antioxidant, antihemolytic and antimicrobial activity",
pages = "73",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4374"
}

Añibarro-Ortega, M., Pinela, J., Ćirić, A., Rocha, F., Barata, A. M., Soković, M., Carvalho, A. M., Ferreira, I. C. F. R.,& Barros, L.. (2021). Table tomato leaves are a sustainable source of rutin and display antioxidant, antihemolytic and antimicrobial activity. in 1st Natural products application: Health, Cosmetic and Food: book of abstracts
Bragança, Portugal: Instituto Politécnico de Bragança (IPB)., 73.
https://hdl.handle.net/21.15107/rcub_ibiss_4374

Añibarro-Ortega M, Pinela J, Ćirić A, Rocha F, Barata AM, Soković M, Carvalho AM, Ferreira ICFR, Barros L. Table tomato leaves are a sustainable source of rutin and display antioxidant, antihemolytic and antimicrobial activity. in 1st Natural products application: Health, Cosmetic and Food: book of abstracts. 2021;:73.
https://hdl.handle.net/21.15107/rcub_ibiss_4374 .

Añibarro-Ortega, Mikel, Pinela, José, Ćirić, Ana, Rocha, Filomena, Barata, Ana Maria, Soković, Marina, Carvalho, Ana Maria, Ferreira, Isabel C. F. R., Barros, Lillian, "Table tomato leaves are a sustainable source of rutin and display antioxidant, antihemolytic and antimicrobial activity" in 1st Natural products application: Health, Cosmetic and Food: book of abstracts (2021):73,
https://hdl.handle.net/21.15107/rcub_ibiss_4374 .

TY  - JOUR
AU  - Añibarro-Ortega, Mikel
AU  - Pinela, José
AU  - Ćirić, Ana
AU  - Martins, Valter
AU  - Rocha, Filomena
AU  - Soković, Marina
AU  - Barata, Ana Maria
AU  - Carvalho, Ana Maria
AU  - Barros, Lillian
AU  - Ferreira, Isabel C.F.R.
PY  - 2020
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0960308520305174
UR  - https://radar.ibiss.bg.ac.rs/123456789/3906
AB  - Little attention has been paid to the phenolic composition and bioactive properties of primary tomato plant by-products (Solanum lycopersicum L.) as compared to fruits. In this study, axillary green shoots resulting from pruning and aerial biomass at the end of the cultivation cycle were characterized for their composition in chlorophylls and phenolic compounds, as well as for antioxidant and antimicrobial activities. The HPLC-DAD-ESI/MSn analysis allowed identifying phenolic acids and flavonoids, with prevalence of quercetin-3-O-rutinoside (rutin). The extracts had in vitro antioxidant and antihemolytic activity, especially those produced from pruning materials. Despite their low activity against the tested microfungi, some extracts had ability to inhibit and kill some bacteria more effectively than the antibiotic ampicillin. It was interesting to conclude that table tomato crop remains, currently with no commercial value, can be used to produce extracts with antioxidant and antibacterial activities for possible use in the agri-food sector as natural preservatives.
PB  - Elsevier BV
T2  - Food and Bioproducts Processing
T1  - Valorisation of table tomato crop by-products: Phenolic profiles and in vitro antioxidant and antimicrobial activities
VL  - 124
DO  - 10.1016/j.fbp.2020.09.006
SP  - 307
EP  - 319
ER  - 

@article{
author = "Añibarro-Ortega, Mikel and Pinela, José and Ćirić, Ana and Martins, Valter and Rocha, Filomena and Soković, Marina and Barata, Ana Maria and Carvalho, Ana Maria and Barros, Lillian and Ferreira, Isabel C.F.R.",
year = "2020",
abstract = "Little attention has been paid to the phenolic composition and bioactive properties of primary tomato plant by-products (Solanum lycopersicum L.) as compared to fruits. In this study, axillary green shoots resulting from pruning and aerial biomass at the end of the cultivation cycle were characterized for their composition in chlorophylls and phenolic compounds, as well as for antioxidant and antimicrobial activities. The HPLC-DAD-ESI/MSn analysis allowed identifying phenolic acids and flavonoids, with prevalence of quercetin-3-O-rutinoside (rutin). The extracts had in vitro antioxidant and antihemolytic activity, especially those produced from pruning materials. Despite their low activity against the tested microfungi, some extracts had ability to inhibit and kill some bacteria more effectively than the antibiotic ampicillin. It was interesting to conclude that table tomato crop remains, currently with no commercial value, can be used to produce extracts with antioxidant and antibacterial activities for possible use in the agri-food sector as natural preservatives.",
publisher = "Elsevier BV",
journal = "Food and Bioproducts Processing",
title = "Valorisation of table tomato crop by-products: Phenolic profiles and in vitro antioxidant and antimicrobial activities",
volume = "124",
doi = "10.1016/j.fbp.2020.09.006",
pages = "307-319"
}

Añibarro-Ortega, M., Pinela, J., Ćirić, A., Martins, V., Rocha, F., Soković, M., Barata, A. M., Carvalho, A. M., Barros, L.,& Ferreira, I. C.F.R.. (2020). Valorisation of table tomato crop by-products: Phenolic profiles and in vitro antioxidant and antimicrobial activities. in Food and Bioproducts Processing
Elsevier BV., 124, 307-319.
https://doi.org/10.1016/j.fbp.2020.09.006

Añibarro-Ortega M, Pinela J, Ćirić A, Martins V, Rocha F, Soković M, Barata AM, Carvalho AM, Barros L, Ferreira IC. Valorisation of table tomato crop by-products: Phenolic profiles and in vitro antioxidant and antimicrobial activities. in Food and Bioproducts Processing. 2020;124:307-319.
doi:10.1016/j.fbp.2020.09.006 .

Añibarro-Ortega, Mikel, Pinela, José, Ćirić, Ana, Martins, Valter, Rocha, Filomena, Soković, Marina, Barata, Ana Maria, Carvalho, Ana Maria, Barros, Lillian, Ferreira, Isabel C.F.R., "Valorisation of table tomato crop by-products: Phenolic profiles and in vitro antioxidant and antimicrobial activities" in Food and Bioproducts Processing, 124 (2020):307-319,
https://doi.org/10.1016/j.fbp.2020.09.006 . .

TY  - JOUR
AU  - Lopes, Catarina L
AU  - Pereira, Eliana
AU  - Soković, Marina
AU  - Carvalho, Ana Maria
AU  - Barata, Ana Maria
AU  - Lopes, Violeta
AU  - Rocha, Filomena
AU  - Calhelha, Ricardo C
AU  - Barros, Lillian
AU  - Ferreira, Isabel C F R
PY  - 2018
UR  - http://www.mdpi.com/1420-3049/23/5/1037
UR  - http://www.ncbi.nlm.nih.gov/pubmed/29710781
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3058
AB  - The aim of this study was to characterize the phenolic composition and evaluate the bioactivity of several samples of Lavandula pedunculata (Mill.) Cav, and to compare aqueous and hydroethanolic extracts. Plant materials were obtained by growing some accessions (seed samples) of various wild populations from different regions of Portugal conserved at the Portuguese Genebank in Braga. Phenolic compounds were analised by HPLC-DAD-ESI/MSn, antioxidant potential through in vitro assays (DPPH radical scavenging activity, reducing power and inhibition of lipid peroxidation), cytotoxicity on tumor cells (MCF-7, NCI-H460, HeLa and HepG2) and non-tumor (PLP2) cells, anti-inflammatory activity in rat RAW 264.7 macrophages, by the ability to inhibit NO production and antimicrobial potential by the microdilution method with INT dye (iodonitrotetrazolium chloride). Thirteen compounds were identified, being salvianolic acid B, rosmarinic acid and luteolin-7-O-glucuronide, the main compounds present, with values ranging between 44.3⁻582, 50.9⁻550, and 24.36⁻101.5 mg/g extract, respectively. L. pedunculata aqueous extract revealed a higher antioxidant potential (EC50 values between 14 to 530 μg/mL), which could be related to its higher concentration in phenolic compounds; however, the hydroethanolic extract showed a higher anti-inflammatory (lower EC50 values than 124 μg/mL) potential and antiproliferative capacity (lower GI50 values than 34 μg/mL). Thus, this study highlights the bioactive effects of this species and opens up possibilities of uses in food and pharmaceutical formulations. However, there are potential differences in such properties according to geographical origin of plant material, as in general, the samples from Alentejo presented higher results in all the bioactivities, compared with Trás-os-Montes samples.
T2  - Molecules (Basel, Switzerland)
T1  - Phenolic Composition and Bioactivity of Lavandula pedunculata (Mill.) Cav. Samples from Different Geographical Origin.
IS  - 5
VL  - 23
DO  - 10.3390/molecules23051037
SP  - 1037
ER  - 

@article{
author = "Lopes, Catarina L and Pereira, Eliana and Soković, Marina and Carvalho, Ana Maria and Barata, Ana Maria and Lopes, Violeta and Rocha, Filomena and Calhelha, Ricardo C and Barros, Lillian and Ferreira, Isabel C F R",
year = "2018",
abstract = "The aim of this study was to characterize the phenolic composition and evaluate the bioactivity of several samples of Lavandula pedunculata (Mill.) Cav, and to compare aqueous and hydroethanolic extracts. Plant materials were obtained by growing some accessions (seed samples) of various wild populations from different regions of Portugal conserved at the Portuguese Genebank in Braga. Phenolic compounds were analised by HPLC-DAD-ESI/MSn, antioxidant potential through in vitro assays (DPPH radical scavenging activity, reducing power and inhibition of lipid peroxidation), cytotoxicity on tumor cells (MCF-7, NCI-H460, HeLa and HepG2) and non-tumor (PLP2) cells, anti-inflammatory activity in rat RAW 264.7 macrophages, by the ability to inhibit NO production and antimicrobial potential by the microdilution method with INT dye (iodonitrotetrazolium chloride). Thirteen compounds were identified, being salvianolic acid B, rosmarinic acid and luteolin-7-O-glucuronide, the main compounds present, with values ranging between 44.3⁻582, 50.9⁻550, and 24.36⁻101.5 mg/g extract, respectively. L. pedunculata aqueous extract revealed a higher antioxidant potential (EC50 values between 14 to 530 μg/mL), which could be related to its higher concentration in phenolic compounds; however, the hydroethanolic extract showed a higher anti-inflammatory (lower EC50 values than 124 μg/mL) potential and antiproliferative capacity (lower GI50 values than 34 μg/mL). Thus, this study highlights the bioactive effects of this species and opens up possibilities of uses in food and pharmaceutical formulations. However, there are potential differences in such properties according to geographical origin of plant material, as in general, the samples from Alentejo presented higher results in all the bioactivities, compared with Trás-os-Montes samples.",
journal = "Molecules (Basel, Switzerland)",
title = "Phenolic Composition and Bioactivity of Lavandula pedunculata (Mill.) Cav. Samples from Different Geographical Origin.",
number = "5",
volume = "23",
doi = "10.3390/molecules23051037",
pages = "1037"
}

Lopes, C. L., Pereira, E., Soković, M., Carvalho, A. M., Barata, A. M., Lopes, V., Rocha, F., Calhelha, R. C., Barros, L.,& Ferreira, I. C. F. R.. (2018). Phenolic Composition and Bioactivity of Lavandula pedunculata (Mill.) Cav. Samples from Different Geographical Origin.. in Molecules (Basel, Switzerland), 23(5), 1037.
https://doi.org/10.3390/molecules23051037

Lopes CL, Pereira E, Soković M, Carvalho AM, Barata AM, Lopes V, Rocha F, Calhelha RC, Barros L, Ferreira ICFR. Phenolic Composition and Bioactivity of Lavandula pedunculata (Mill.) Cav. Samples from Different Geographical Origin.. in Molecules (Basel, Switzerland). 2018;23(5):1037.
doi:10.3390/molecules23051037 .

Lopes, Catarina L, Pereira, Eliana, Soković, Marina, Carvalho, Ana Maria, Barata, Ana Maria, Lopes, Violeta, Rocha, Filomena, Calhelha, Ricardo C, Barros, Lillian, Ferreira, Isabel C F R, "Phenolic Composition and Bioactivity of Lavandula pedunculata (Mill.) Cav. Samples from Different Geographical Origin." in Molecules (Basel, Switzerland), 23, no. 5 (2018):1037,
https://doi.org/10.3390/molecules23051037 . .