TY  - JOUR
AU  - Drvenica, Ivana T.
AU  - Bukara, Katarina M.
AU  - Ilić, Vesna Lj.
AU  - Mišić, Danijela
AU  - Vasić, Borislav Z.
AU  - Gajić, Radoš B.
AU  - Đorđević, Verica B.
AU  - Veljović, Đorđe N.
AU  - Belić, Aleksandar
AU  - Bugarski, Branko M.
PY  - 2016
UR  - http://doi.wiley.com/10.1002/btpr.2304
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2541
AB  - The present study investigated preparation of bovine and porcine erythrocyte membranes from slaughterhouse blood as bio-derived materials for delivery of dexamethasone-sodium phosphate (DexP). The obtained biomembranes, i.e., ghosts were characterized in vitro in terms of morphological properties, loading parameters, and release behavior. For the last two, an UHPLC/-HESI-MS/MS based analytical procedure for absolute drug identification and quantification was developed. The results revealed that loading of DexP into both type of ghosts was directly proportional to the increase of drug concentration in the incubation medium, while incubation at 37°C had statistically significant effect on loaded amount of DexP (P<0.05). The encapsulation efficiency was about fivefold higher in porcine compared to bovine ghosts. Insight into ghosts' surface morphology by field emission-scanning electron microscopy and atomic force microscopy confirmed that besides inevitable effects of osmosis, DexP inclusion itself had no observable additional effect on the morphology of the ghosts carriers. DexP release profiles were dependent on erythrocyte ghost type and amount of residual hemoglobin. However, sustained DexP release was achieved and shown over 3 days from porcine ghosts and 5 days from bovine erythrocyte ghosts.
T2  - Biotechnology Progress
T1  - Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate
IS  - 4
VL  - 32
DO  - 10.1002/btpr.2304
SP  - 1046
EP  - 1055
ER  - 

@article{
author = "Drvenica, Ivana T. and Bukara, Katarina M. and Ilić, Vesna Lj. and Mišić, Danijela and Vasić, Borislav Z. and Gajić, Radoš B. and Đorđević, Verica B. and Veljović, Đorđe N. and Belić, Aleksandar and Bugarski, Branko M.",
year = "2016",
abstract = "The present study investigated preparation of bovine and porcine erythrocyte membranes from slaughterhouse blood as bio-derived materials for delivery of dexamethasone-sodium phosphate (DexP). The obtained biomembranes, i.e., ghosts were characterized in vitro in terms of morphological properties, loading parameters, and release behavior. For the last two, an UHPLC/-HESI-MS/MS based analytical procedure for absolute drug identification and quantification was developed. The results revealed that loading of DexP into both type of ghosts was directly proportional to the increase of drug concentration in the incubation medium, while incubation at 37°C had statistically significant effect on loaded amount of DexP (P<0.05). The encapsulation efficiency was about fivefold higher in porcine compared to bovine ghosts. Insight into ghosts' surface morphology by field emission-scanning electron microscopy and atomic force microscopy confirmed that besides inevitable effects of osmosis, DexP inclusion itself had no observable additional effect on the morphology of the ghosts carriers. DexP release profiles were dependent on erythrocyte ghost type and amount of residual hemoglobin. However, sustained DexP release was achieved and shown over 3 days from porcine ghosts and 5 days from bovine erythrocyte ghosts.",
journal = "Biotechnology Progress",
title = "Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate",
number = "4",
volume = "32",
doi = "10.1002/btpr.2304",
pages = "1046-1055"
}

Drvenica, I. T., Bukara, K. M., Ilić, V. Lj., Mišić, D., Vasić, B. Z., Gajić, R. B., Đorđević, V. B., Veljović, Đ. N., Belić, A.,& Bugarski, B. M.. (2016). Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate. in Biotechnology Progress, 32(4), 1046-1055.
https://doi.org/10.1002/btpr.2304

Drvenica IT, Bukara KM, Ilić VL, Mišić D, Vasić BZ, Gajić RB, Đorđević VB, Veljović ĐN, Belić A, Bugarski BM. Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate. in Biotechnology Progress. 2016;32(4):1046-1055.
doi:10.1002/btpr.2304 .

Drvenica, Ivana T., Bukara, Katarina M., Ilić, Vesna Lj., Mišić, Danijela, Vasić, Borislav Z., Gajić, Radoš B., Đorđević, Verica B., Veljović, Đorđe N., Belić, Aleksandar, Bugarski, Branko M., "Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate" in Biotechnology Progress, 32, no. 4 (2016):1046-1055,
https://doi.org/10.1002/btpr.2304 . .

TY  - JOUR
AU  - Kostic, Ivana T.
AU  - Ilic, Vesna Lj.
AU  - Đorđević, Verica B.
AU  - Bukara, Katarina M.
AU  - Mojsilovic, Slavko B.
AU  - Nedovic, Viktor A.
AU  - Bugarski, Diana S.
AU  - Veljovic, Dorde N.
AU  - Misic, Danijela M.
AU  - Bugarski, Branko M.
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2145
AB  - The present study was aimed at investigating the effect of isolation
   process-gradual hypotonic hemolysis on chosen parameters of the
   erythrocyte membranes (ghosts) originating from bovine and porcine
   slaughterhouse blood. The estimation of the gradual hypotonic hemolysis
   as a drug loading procedure for the erythrocyte ghosts was performed as
   well.
   Based on the results derived from analysis of the osmotic properties of
   the erythrocytes, the gradual hemolysis was performed with high volume
   of erythrocytes and 35 mM hypotonic sodium-phosphate/NaCl, enabling
   >90\% of hemolysis for both types of erythrocytes. Detailed insight into
   ghosts' morphology by field emission-scanning electron microscopy
   revealed a distortion from erythrocyte shape and an altered surface
   texture with increased bilayer curvature for both samples. Compared to
   erythrocytes, an average diameter of ghosts from both type of
   erythrocytes decreased for only about 10\%. The reported unidispersity
   of the isolated ghosts is of great importance for their potential
   application as vehicles of active compounds. Gradual hemolysis did not
   lead to substantial loss of cholesterol and membrane/cytoskeleton
   proteins. This result indicated the ghosts' possibility to mimic the
   chemical and structural anisotropic environment of in vivo cell
   membranes, which is of significance for drug diffusion and partition
   coefficients. Induced shift of phosphatidylserine to external surface of
   the ghosts demonstrated their potential application as vehicles for
   targeted drug delivery to cells of reticuloendothelial system. Ultra
   high-performance liquid chromatography and Fourier transform infrared
   spectroscopy revealed the presence of a drug model -
   dexamethasone-sodium phosphate, and its interaction with structural
   components in both types of erythrocyte ghosts. (C) 2014 Elsevier B.V.
   All rights reserved.
T2  - Colloids and Surfaces B-Biointerfaces
T1  - Erythrocyte membranes from slaughterhouse blood as potential drug
 vehicles: Isolation by gradual hypotonic hemolysis and biochemical and
 morphological characterization
VL  - 122
DO  - 10.1016/j.colsurfb.2014.06.043
SP  - 250
EP  - 259
ER  - 

@article{
author = "Kostic, Ivana T. and Ilic, Vesna Lj. and Đorđević, Verica B. and Bukara, Katarina M. and Mojsilovic, Slavko B. and Nedovic, Viktor A. and Bugarski, Diana S. and Veljovic, Dorde N. and Misic, Danijela M. and Bugarski, Branko M.",
year = "2014",
abstract = "The present study was aimed at investigating the effect of isolation
   process-gradual hypotonic hemolysis on chosen parameters of the
   erythrocyte membranes (ghosts) originating from bovine and porcine
   slaughterhouse blood. The estimation of the gradual hypotonic hemolysis
   as a drug loading procedure for the erythrocyte ghosts was performed as
   well.
   Based on the results derived from analysis of the osmotic properties of
   the erythrocytes, the gradual hemolysis was performed with high volume
   of erythrocytes and 35 mM hypotonic sodium-phosphate/NaCl, enabling
   >90\% of hemolysis for both types of erythrocytes. Detailed insight into
   ghosts' morphology by field emission-scanning electron microscopy
   revealed a distortion from erythrocyte shape and an altered surface
   texture with increased bilayer curvature for both samples. Compared to
   erythrocytes, an average diameter of ghosts from both type of
   erythrocytes decreased for only about 10\%. The reported unidispersity
   of the isolated ghosts is of great importance for their potential
   application as vehicles of active compounds. Gradual hemolysis did not
   lead to substantial loss of cholesterol and membrane/cytoskeleton
   proteins. This result indicated the ghosts' possibility to mimic the
   chemical and structural anisotropic environment of in vivo cell
   membranes, which is of significance for drug diffusion and partition
   coefficients. Induced shift of phosphatidylserine to external surface of
   the ghosts demonstrated their potential application as vehicles for
   targeted drug delivery to cells of reticuloendothelial system. Ultra
   high-performance liquid chromatography and Fourier transform infrared
   spectroscopy revealed the presence of a drug model -
   dexamethasone-sodium phosphate, and its interaction with structural
   components in both types of erythrocyte ghosts. (C) 2014 Elsevier B.V.
   All rights reserved.",
journal = "Colloids and Surfaces B-Biointerfaces",
title = "Erythrocyte membranes from slaughterhouse blood as potential drug
 vehicles: Isolation by gradual hypotonic hemolysis and biochemical and
 morphological characterization",
volume = "122",
doi = "10.1016/j.colsurfb.2014.06.043",
pages = "250-259"
}

Kostic, I. T., Ilic, V. Lj., Đorđević, V. B., Bukara, K. M., Mojsilovic, S. B., Nedovic, V. A., Bugarski, D. S., Veljovic, D. N., Misic, D. M.,& Bugarski, B. M.. (2014). Erythrocyte membranes from slaughterhouse blood as potential drug
 vehicles: Isolation by gradual hypotonic hemolysis and biochemical and
 morphological characterization. in Colloids and Surfaces B-Biointerfaces, 122, 250-259.
https://doi.org/10.1016/j.colsurfb.2014.06.043

Kostic IT, Ilic VL, Đorđević VB, Bukara KM, Mojsilovic SB, Nedovic VA, Bugarski DS, Veljovic DN, Misic DM, Bugarski BM. Erythrocyte membranes from slaughterhouse blood as potential drug
 vehicles: Isolation by gradual hypotonic hemolysis and biochemical and
 morphological characterization. in Colloids and Surfaces B-Biointerfaces. 2014;122:250-259.
doi:10.1016/j.colsurfb.2014.06.043 .

Kostic, Ivana T., Ilic, Vesna Lj., Đorđević, Verica B., Bukara, Katarina M., Mojsilovic, Slavko B., Nedovic, Viktor A., Bugarski, Diana S., Veljovic, Dorde N., Misic, Danijela M., Bugarski, Branko M., "Erythrocyte membranes from slaughterhouse blood as potential drug
 vehicles: Isolation by gradual hypotonic hemolysis and biochemical and
 morphological characterization" in Colloids and Surfaces B-Biointerfaces, 122 (2014):250-259,
https://doi.org/10.1016/j.colsurfb.2014.06.043 . .