TY  - JOUR
AU  - Šumarac-Dumanović, Mirjana
AU  - Apostolović, Milica
AU  - Janjetović, Kristina
AU  - Jeremić, Danka
AU  - Popadić, Dušan
AU  - Ljubić, Aleksandar
AU  - Micić, Jelena
AU  - Dukanac-Stamenković, Jelena
AU  - Tubić, Aleksandra
AU  - Stevanović, Darko
AU  - Micić, Dragan
AU  - Trajković, Vladimir
PY  - 2017
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6352
AB  - Autophagy, a process of controlled cellular self-digestion, could be involved in cyclic remodeling
of the human endometrium. We investigated endometrial mRNA expression of 23 autophagyrelated
(ATG) genes and transcription factors in healthy controls (n = 12) and anovulatory
polycystic ovary syndrome (PCOS) patients (n = 24), as well as in their subgroup (n = 12) before
and after metformin treatment. The mRNA levels of transcription factor forkhead box protein O1
(FOXO1) and several molecules involved in autophagosome formation (ATG13, RB1-inducible
coiled-coil 1), autophagosome nucleation (ATG14, beclin 1, SH3-domain GRB2-like endophilin
B1), autophagosome elongation (ATG3, ATG5, g-aminobutyric acid receptor-associated protein -
GABARAP), and delivery of ubiquitinated proteins to autophagosomes (sequestosome 1), were
significantly reduced in anovulatory PCOS compared to healthy endometrium. Free androgen
index, but not free estrogen index, insulin levels, or BMI, negatively correlated with the
endometrial expression of ATG3, ATG14, and GABARAP in PCOS patients. Treatment of
PCOS patients with metformin (2 g/day for 3 months) significantly increased the endometrial
mRNA levels of FOXO1, ATG3, and UV radiation resistance-associated gene. These data
suggest that increased androgen availability in PCOS is associated with metformin-sensitive
transcriptional downregulation of endometrial autophagy
PB  - Elsevier
T2  - Molecular and Cellular Endocrinology
T1  - Downregulation of autophagy gene expression in endometria from women with polycystic ovary syndrome
VL  - 440
DO  - 10.1016/j.mce.2016.11.009
SP  - 116
EP  - 124
ER  - 

@article{
author = "Šumarac-Dumanović, Mirjana and Apostolović, Milica and Janjetović, Kristina and Jeremić, Danka and Popadić, Dušan and Ljubić, Aleksandar and Micić, Jelena and Dukanac-Stamenković, Jelena and Tubić, Aleksandra and Stevanović, Darko and Micić, Dragan and Trajković, Vladimir",
year = "2017",
abstract = "Autophagy, a process of controlled cellular self-digestion, could be involved in cyclic remodeling
of the human endometrium. We investigated endometrial mRNA expression of 23 autophagyrelated
(ATG) genes and transcription factors in healthy controls (n = 12) and anovulatory
polycystic ovary syndrome (PCOS) patients (n = 24), as well as in their subgroup (n = 12) before
and after metformin treatment. The mRNA levels of transcription factor forkhead box protein O1
(FOXO1) and several molecules involved in autophagosome formation (ATG13, RB1-inducible
coiled-coil 1), autophagosome nucleation (ATG14, beclin 1, SH3-domain GRB2-like endophilin
B1), autophagosome elongation (ATG3, ATG5, g-aminobutyric acid receptor-associated protein -
GABARAP), and delivery of ubiquitinated proteins to autophagosomes (sequestosome 1), were
significantly reduced in anovulatory PCOS compared to healthy endometrium. Free androgen
index, but not free estrogen index, insulin levels, or BMI, negatively correlated with the
endometrial expression of ATG3, ATG14, and GABARAP in PCOS patients. Treatment of
PCOS patients with metformin (2 g/day for 3 months) significantly increased the endometrial
mRNA levels of FOXO1, ATG3, and UV radiation resistance-associated gene. These data
suggest that increased androgen availability in PCOS is associated with metformin-sensitive
transcriptional downregulation of endometrial autophagy",
publisher = "Elsevier",
journal = "Molecular and Cellular Endocrinology",
title = "Downregulation of autophagy gene expression in endometria from women with polycystic ovary syndrome",
volume = "440",
doi = "10.1016/j.mce.2016.11.009",
pages = "116-124"
}

Šumarac-Dumanović, M., Apostolović, M., Janjetović, K., Jeremić, D., Popadić, D., Ljubić, A., Micić, J., Dukanac-Stamenković, J., Tubić, A., Stevanović, D., Micić, D.,& Trajković, V.. (2017). Downregulation of autophagy gene expression in endometria from women with polycystic ovary syndrome. in Molecular and Cellular Endocrinology
Elsevier., 440, 116-124.
https://doi.org/10.1016/j.mce.2016.11.009

Šumarac-Dumanović M, Apostolović M, Janjetović K, Jeremić D, Popadić D, Ljubić A, Micić J, Dukanac-Stamenković J, Tubić A, Stevanović D, Micić D, Trajković V. Downregulation of autophagy gene expression in endometria from women with polycystic ovary syndrome. in Molecular and Cellular Endocrinology. 2017;440:116-124.
doi:10.1016/j.mce.2016.11.009 .

Šumarac-Dumanović, Mirjana, Apostolović, Milica, Janjetović, Kristina, Jeremić, Danka, Popadić, Dušan, Ljubić, Aleksandar, Micić, Jelena, Dukanac-Stamenković, Jelena, Tubić, Aleksandra, Stevanović, Darko, Micić, Dragan, Trajković, Vladimir, "Downregulation of autophagy gene expression in endometria from women with polycystic ovary syndrome" in Molecular and Cellular Endocrinology, 440 (2017):116-124,
https://doi.org/10.1016/j.mce.2016.11.009 . .