@article{
author = "Golic, I. and Velickovic, K. and Markelic, M. and Stancic, A. and Jankovic, A. and Vucetic, M. and Otašević, Vesna and Buzadžić, Biljana J. and Korać, Bato and Korac, A.",
year = "2014",
abstract = "Mitochondria are key organelles maintaining cellular bioenergetics and
integrity, and their regulation of {[}Ca2+](i) homeostasis has been
investigated in many cell types. We investigated the short-term
Ca-SANDOZ (R) treatment on brown adipocyte mitochondria, using imaging
and molecular biology techniques. Two-month-old male Wistar rats were
divided into two groups: Ca-SANDOZ (R) drinking or tap water (control)
drinking for three days. Alizarin Red S staining showed increased Ca2+
level in the brown adipocytes of treated rats, and potassium
pyroantimonate staining localized electron-dense regions in the
cytoplasm, mitochondria and around lipid droplets. Ca-SANDOZ (R)
decreased mitochondrial number, but increased their size and
mitochondrial cristae volume. Transmission electron microscopy revealed
numerous enlarged and fusioned-like mitochondria in the Ca-SANDOZ (R)
treated group compared to the control, and megamitochondria in some
brown adipocytes. The Ca2+ diet affected mitochondrial fusion as
mitofusin 1 (MFN1) and mitofusin 2 (MFN2) were increased, and
mitochondrial fission as dynamin related protein 1 (DRP1) was decreased.
Confocal microscopy showed a higher colocalization rate between
functional mitochondria and endoplasmic reticulum (ER). The level of
uncoupling protein-1 (UCP1) was elevated, which was confirmed by
immunohistochemistry and Western blot analysis. These results suggest
that Ca-SANDOZ (R) stimulates mitochondrial fusion, increases
mitochondrial-ER contacts and the thermogenic capacity of brown
adipocytes.",
journal = "European Journal of Histochemistry",
title = "Calcium-induced alteration of mitochondrial morphology and
mitochondrial-endoplasmic reticulum contacts in rat brown adipocytes",
number = "2377",
volume = "58",
doi = "10.4081/ejh.2014.2377",
pages = "251-257"
}
