TY  - JOUR
AU  - Stošić-Grujičić, Stanislava
AU  - Maksimović-Ivanić, Danijela
AU  - Badovinac, Vladimir
AU  - Samardžić, Tatjana S.
AU  - Trajković, Vladimir S
AU  - Lukić, Miodrag L
AU  - Mostarica-Stojković, Marija B
PY  - 2001
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1821
AB  - We have shown recently that xanthine derivative pentoxifylline (PTX) downregulates an inflammatory autoimmune process triggered in genetically susceptible Dark Agouti rats by multiple low doses of streptozotocin (MLD-SZ, 20 mg/kg/day ip for 5 days). We studied the cellular and molecular consequences of PTX treatment during MLD-SZ-induced diabetes with special emphasis on local vs. systemic production of inflammatory mediators. Administration of PTX (200 mg/kg/day for 10 days) during induction of the disease reduced clinical signs of diabetes and protected rats from development of destructive intrainsulitis. Pentoxifylline did not affect diabetogenic effect of single high dose of SZ (100 mg/kg SZ). Ex vivo analysis of the islets of Langerhans performed in early disease development revealed that PTX downregulates production of proinflammatory cytokines IFN-gamma and TNF, as well as inducible nitric oxide synthase (iNOS) expression and NO production. In addition, PTX treatment suppressed splenocyte autoreactivity, as well as the frequency of cells expressing IL-2R and MHC class II antigens. There was no evidence of any changes in proportion of ICAM-1 and LFA-1 expressing splenocytes in comparison to control MLD-SZ-treated animals. In contrast to suppressed intraislet production, high peripheral expression of both iNOS mRNA and NO was found in MLD-SZ rats treated with PTX. Taken together, the data indicate that the effect on both systemic and intra-islet production of NO, suppression of autoreactive cell activation and of local type 1 cytokine release may contribute to the therapeutic benefit achieved by PTX in the rat. (C) 2001 Academic Press.
T2  - Journal of Autoimmunity
T1  - Antidiabetogenic effect of pentoxifylline is associated with systemic and target tissue modulation of cytokines and nitric oxide production
IS  - 1
VL  - 16
EP  - 58
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1821
ER  - 

@article{
author = "Stošić-Grujičić, Stanislava and Maksimović-Ivanić, Danijela and Badovinac, Vladimir and Samardžić, Tatjana S. and Trajković, Vladimir S and Lukić, Miodrag L and Mostarica-Stojković, Marija B",
year = "2001",
abstract = "We have shown recently that xanthine derivative pentoxifylline (PTX) downregulates an inflammatory autoimmune process triggered in genetically susceptible Dark Agouti rats by multiple low doses of streptozotocin (MLD-SZ, 20 mg/kg/day ip for 5 days). We studied the cellular and molecular consequences of PTX treatment during MLD-SZ-induced diabetes with special emphasis on local vs. systemic production of inflammatory mediators. Administration of PTX (200 mg/kg/day for 10 days) during induction of the disease reduced clinical signs of diabetes and protected rats from development of destructive intrainsulitis. Pentoxifylline did not affect diabetogenic effect of single high dose of SZ (100 mg/kg SZ). Ex vivo analysis of the islets of Langerhans performed in early disease development revealed that PTX downregulates production of proinflammatory cytokines IFN-gamma and TNF, as well as inducible nitric oxide synthase (iNOS) expression and NO production. In addition, PTX treatment suppressed splenocyte autoreactivity, as well as the frequency of cells expressing IL-2R and MHC class II antigens. There was no evidence of any changes in proportion of ICAM-1 and LFA-1 expressing splenocytes in comparison to control MLD-SZ-treated animals. In contrast to suppressed intraislet production, high peripheral expression of both iNOS mRNA and NO was found in MLD-SZ rats treated with PTX. Taken together, the data indicate that the effect on both systemic and intra-islet production of NO, suppression of autoreactive cell activation and of local type 1 cytokine release may contribute to the therapeutic benefit achieved by PTX in the rat. (C) 2001 Academic Press.",
journal = "Journal of Autoimmunity",
title = "Antidiabetogenic effect of pentoxifylline is associated with systemic and target tissue modulation of cytokines and nitric oxide production",
number = "1",
volume = "16",
pages = "58",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1821"
}

Stošić-Grujičić, S., Maksimović-Ivanić, D., Badovinac, V., Samardžić, T. S., Trajković, V. S., Lukić, M. L.,& Mostarica-Stojković, M. B.. (2001). Antidiabetogenic effect of pentoxifylline is associated with systemic and target tissue modulation of cytokines and nitric oxide production. in Journal of Autoimmunity, 16(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1821

Stošić-Grujičić S, Maksimović-Ivanić D, Badovinac V, Samardžić TS, Trajković VS, Lukić ML, Mostarica-Stojković MB. Antidiabetogenic effect of pentoxifylline is associated with systemic and target tissue modulation of cytokines and nitric oxide production. in Journal of Autoimmunity. 2001;16(1):null-58.
https://hdl.handle.net/21.15107/rcub_ibiss_1821 .

Stošić-Grujičić, Stanislava, Maksimović-Ivanić, Danijela, Badovinac, Vladimir, Samardžić, Tatjana S., Trajković, Vladimir S, Lukić, Miodrag L, Mostarica-Stojković, Marija B, "Antidiabetogenic effect of pentoxifylline is associated with systemic and target tissue modulation of cytokines and nitric oxide production" in Journal of Autoimmunity, 16, no. 1 (2001),
https://hdl.handle.net/21.15107/rcub_ibiss_1821 .

TY  - JOUR
AU  - Trajković, Vladimir S
AU  - Stepanović, Srđan
AU  - Samardžić, Tatjana S.
AU  - Janković, V
AU  - Badovinac, Vladimir
AU  - Mostarica-Stojković, Marija B
PY  - 2000
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1833
AB  - The effect of Cryptococcus neoformans on the accumulation of nitrite, an indicator of nitric oxide (NO) synthesis, was investigated in cytokine (interferon-gamma [IFN-gamma] and interleukin [IL]-1)-stimulated cultures of rat peritoneal macrophages and C6 astrocytoma cells. Cytokine-induced nitrite generation in cultures of both cell types was inhibited in a dose-dependent manner by live C. neoformans, but not by heat-killed cryptococcal cells or conditioned medium from yeast cultures. C. neoformans-mediated reduction of nitrite formation coincided with impairment of NO-dependent macrophage tumoricidal activity. Cytokine-triggered induction of inducible NO synthase (iNOS) was unaffected in C6 cells, and only marginally reduced in macrophages. When cells were pretreated with cytokines for 24 h to induce iNOS, and any further induction was prevented by inhibition of protein synthesis, C. neoformans was still able to reduce nitrite accumulation in cultures of both cell types. Finally, live C. neoformans, but not heat-killed yeast cells or yeast culture supernatant, significantly reduced nitrite production in a culture solution of NO-releasing compound S-nitrosoglutathione (GSNO). Thus, it appears that cryptococcal reduction of nitrite formation in macrophage and C6 cultures was caused by the consumption of NO by some yeast molecule, rather than by the inhibition of cellular NO synthesis.
T2  - Scandinavian Journal of Immunology
T1  - Cryptococcus neoformans neutralizes macrophage and astrocyte derived nitric oxide without interfering with inducible nitric oxide synthase induction or catalytic activity - Possible involvement of nitric oxide consumption
IS  - 4
VL  - 51
EP  - 391
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1833
ER  - 

@article{
author = "Trajković, Vladimir S and Stepanović, Srđan and Samardžić, Tatjana S. and Janković, V and Badovinac, Vladimir and Mostarica-Stojković, Marija B",
year = "2000",
abstract = "The effect of Cryptococcus neoformans on the accumulation of nitrite, an indicator of nitric oxide (NO) synthesis, was investigated in cytokine (interferon-gamma [IFN-gamma] and interleukin [IL]-1)-stimulated cultures of rat peritoneal macrophages and C6 astrocytoma cells. Cytokine-induced nitrite generation in cultures of both cell types was inhibited in a dose-dependent manner by live C. neoformans, but not by heat-killed cryptococcal cells or conditioned medium from yeast cultures. C. neoformans-mediated reduction of nitrite formation coincided with impairment of NO-dependent macrophage tumoricidal activity. Cytokine-triggered induction of inducible NO synthase (iNOS) was unaffected in C6 cells, and only marginally reduced in macrophages. When cells were pretreated with cytokines for 24 h to induce iNOS, and any further induction was prevented by inhibition of protein synthesis, C. neoformans was still able to reduce nitrite accumulation in cultures of both cell types. Finally, live C. neoformans, but not heat-killed yeast cells or yeast culture supernatant, significantly reduced nitrite production in a culture solution of NO-releasing compound S-nitrosoglutathione (GSNO). Thus, it appears that cryptococcal reduction of nitrite formation in macrophage and C6 cultures was caused by the consumption of NO by some yeast molecule, rather than by the inhibition of cellular NO synthesis.",
journal = "Scandinavian Journal of Immunology",
title = "Cryptococcus neoformans neutralizes macrophage and astrocyte derived nitric oxide without interfering with inducible nitric oxide synthase induction or catalytic activity - Possible involvement of nitric oxide consumption",
number = "4",
volume = "51",
pages = "391",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1833"
}

Trajković, V. S., Stepanović, S., Samardžić, T. S., Janković, V., Badovinac, V.,& Mostarica-Stojković, M. B.. (2000). Cryptococcus neoformans neutralizes macrophage and astrocyte derived nitric oxide without interfering with inducible nitric oxide synthase induction or catalytic activity - Possible involvement of nitric oxide consumption. in Scandinavian Journal of Immunology, 51(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1833

Trajković VS, Stepanović S, Samardžić TS, Janković V, Badovinac V, Mostarica-Stojković MB. Cryptococcus neoformans neutralizes macrophage and astrocyte derived nitric oxide without interfering with inducible nitric oxide synthase induction or catalytic activity - Possible involvement of nitric oxide consumption. in Scandinavian Journal of Immunology. 2000;51(4):null-391.
https://hdl.handle.net/21.15107/rcub_ibiss_1833 .

Trajković, Vladimir S, Stepanović, Srđan, Samardžić, Tatjana S., Janković, V, Badovinac, Vladimir, Mostarica-Stojković, Marija B, "Cryptococcus neoformans neutralizes macrophage and astrocyte derived nitric oxide without interfering with inducible nitric oxide synthase induction or catalytic activity - Possible involvement of nitric oxide consumption" in Scandinavian Journal of Immunology, 51, no. 4 (2000),
https://hdl.handle.net/21.15107/rcub_ibiss_1833 .