TY  - JOUR
AU  - Oreščanin Dušić, Zorana
AU  - Miljević, Cedo D
AU  - Slavić, Marija
AU  - Nikolić-Kokić, Aleksandra
AU  - Blagojević, Duško
AU  - Lecić-Tosevski, Dusica M
AU  - Spasić, Mihajlo
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1172
AB  - Tianeptine is a novel anti-depressant with an efficacy equivalent to that of classical anti-depressants. Additional beneficial effects include neuroprotection, anti-stress and anti-ulcer properties whose molecular mechanisms are still not completely understood but may involve changes in the anti-oxidant defence system. Herein, we have studied the effects of tianeptine on both contractile activity of isolated rat uteri and components of the endogenous anti-oxidative defence system. Tianeptine-induced dose-dependent inhibition of both spontaneous and Ca2+-induced contraction of uterine smooth muscle. The effect was more pronounced in the latter. Tianeptine treatment increased glutathione-peroxidase (GSH-Px) and catalase (CAT) activities in spontaneous and Ca2+-stimulated uteri. A significant decrease in glutathione-reductase (GR) activity in both spontaneous and Ca2+-induced uterine contractions after tianeptine treatment indicated a reduction in reduced glutathione and consequently a shift toward a more oxidised state in the treated uteri. In spontaneously contracting uteri, tianeptine caused a decrease in copper-zinc SOD (CuZnSOD) activity. Tianeptine's anti-depressant effects may be accomplished by triggering a cascade of cellular adaptations including inhibition of smooth muscle contractility and an adequate anti-oxidative protection response.
T2  - Acta Physiologica Hungarica
T1  - Tianeptine's effects on spontaneous and Ca2+-induced uterine smooth muscle contraction
IS  - 2
VL  - 99
EP  - 147
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1172
ER  - 

@article{
author = "Oreščanin Dušić, Zorana and Miljević, Cedo D and Slavić, Marija and Nikolić-Kokić, Aleksandra and Blagojević, Duško and Lecić-Tosevski, Dusica M and Spasić, Mihajlo",
year = "2012",
abstract = "Tianeptine is a novel anti-depressant with an efficacy equivalent to that of classical anti-depressants. Additional beneficial effects include neuroprotection, anti-stress and anti-ulcer properties whose molecular mechanisms are still not completely understood but may involve changes in the anti-oxidant defence system. Herein, we have studied the effects of tianeptine on both contractile activity of isolated rat uteri and components of the endogenous anti-oxidative defence system. Tianeptine-induced dose-dependent inhibition of both spontaneous and Ca2+-induced contraction of uterine smooth muscle. The effect was more pronounced in the latter. Tianeptine treatment increased glutathione-peroxidase (GSH-Px) and catalase (CAT) activities in spontaneous and Ca2+-stimulated uteri. A significant decrease in glutathione-reductase (GR) activity in both spontaneous and Ca2+-induced uterine contractions after tianeptine treatment indicated a reduction in reduced glutathione and consequently a shift toward a more oxidised state in the treated uteri. In spontaneously contracting uteri, tianeptine caused a decrease in copper-zinc SOD (CuZnSOD) activity. Tianeptine's anti-depressant effects may be accomplished by triggering a cascade of cellular adaptations including inhibition of smooth muscle contractility and an adequate anti-oxidative protection response.",
journal = "Acta Physiologica Hungarica",
title = "Tianeptine's effects on spontaneous and Ca2+-induced uterine smooth muscle contraction",
number = "2",
volume = "99",
pages = "147",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1172"
}

Oreščanin Dušić, Z., Miljević, C. D., Slavić, M., Nikolić-Kokić, A., Blagojević, D., Lecić-Tosevski, D. M.,& Spasić, M.. (2012). Tianeptine's effects on spontaneous and Ca2+-induced uterine smooth muscle contraction. in Acta Physiologica Hungarica, 99(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1172

Oreščanin Dušić Z, Miljević CD, Slavić M, Nikolić-Kokić A, Blagojević D, Lecić-Tosevski DM, Spasić M. Tianeptine's effects on spontaneous and Ca2+-induced uterine smooth muscle contraction. in Acta Physiologica Hungarica. 2012;99(2):null-147.
https://hdl.handle.net/21.15107/rcub_ibiss_1172 .

Oreščanin Dušić, Zorana, Miljević, Cedo D, Slavić, Marija, Nikolić-Kokić, Aleksandra, Blagojević, Duško, Lecić-Tosevski, Dusica M, Spasić, Mihajlo, "Tianeptine's effects on spontaneous and Ca2+-induced uterine smooth muscle contraction" in Acta Physiologica Hungarica, 99, no. 2 (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1172 .

TY  - JOUR
AU  - Miljević, Cedo D
AU  - Nikolić-Kokić, Aleksandra
AU  - Saičić, Zorica
AU  - Milosavljević, Maja
AU  - Blagojević, Duško
AU  - Lecić-Tosevski, Dusica M
AU  - Jones, David R
AU  - Spasić, Mihajlo
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1365
AB  - The activities of antioxidant defence enzymes were determined in erythrocytes isolated from types I and II schizophrenic male patients and from healthy controls. Significant differences in superoxide dismutase (SOD) activity (type I: 3284 +/- 577; type II: 2959 +/- 697 compared with controls: 3778 +/- 577; analysis of variance (ANOVA) P<0.001), catalase (CAT) activity (type I: 17.8 +/- 1.8 compared to type II: 19.2 +/- 1.5 and both compared with controls: 19.2 +/- 1.5; ANOVA P<0.05). glutathione peroxidase (GSH-Px) activity (controls: 17.8 +/- 2.3; type I: 13.9 +/- 2.9 and type II: 11.6 +/- 1.9; ANOVA P<0.001) as well as in glutathione reductase (GR) activity (controls: 5.0 +/- 0.8; type I: 4.3 +/- 0.9 and type II: 4.5 +/- 0.8; ANOVA P<0.01) were apparent. Correlation analysis of antioxidant defence enzymes showed significant negative correlation between GSH-Px and CAT activities (P<0.01) in type I patients. In type II patients, GSH-Px activity was significantly positively correlated with GR (P<0.01). Canonical discriminant analysis separated type I and type II patients from controls (and among each other) with a high degree of certainty according to the overall group composition of antioxidant defence enzymes. Our results indicate differences in the composition of antioxidant defence between controls and antipsychotic treated type I and type II patients with a possible negative feedback influence on the pathological process, which could provide a rationale for applying antioxidants during schizophrenic therapy. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
T2  - Psychiatry Research
T1  - Correlation analysis confirms differences in antioxidant defence in the blood of types I and II schizophrenic male patients treated with anti-psychotic medication
IS  - 1
VL  - 178
EP  - 72
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1365
ER  - 

@article{
author = "Miljević, Cedo D and Nikolić-Kokić, Aleksandra and Saičić, Zorica and Milosavljević, Maja and Blagojević, Duško and Lecić-Tosevski, Dusica M and Jones, David R and Spasić, Mihajlo",
year = "2010",
abstract = "The activities of antioxidant defence enzymes were determined in erythrocytes isolated from types I and II schizophrenic male patients and from healthy controls. Significant differences in superoxide dismutase (SOD) activity (type I: 3284 +/- 577; type II: 2959 +/- 697 compared with controls: 3778 +/- 577; analysis of variance (ANOVA) P<0.001), catalase (CAT) activity (type I: 17.8 +/- 1.8 compared to type II: 19.2 +/- 1.5 and both compared with controls: 19.2 +/- 1.5; ANOVA P<0.05). glutathione peroxidase (GSH-Px) activity (controls: 17.8 +/- 2.3; type I: 13.9 +/- 2.9 and type II: 11.6 +/- 1.9; ANOVA P<0.001) as well as in glutathione reductase (GR) activity (controls: 5.0 +/- 0.8; type I: 4.3 +/- 0.9 and type II: 4.5 +/- 0.8; ANOVA P<0.01) were apparent. Correlation analysis of antioxidant defence enzymes showed significant negative correlation between GSH-Px and CAT activities (P<0.01) in type I patients. In type II patients, GSH-Px activity was significantly positively correlated with GR (P<0.01). Canonical discriminant analysis separated type I and type II patients from controls (and among each other) with a high degree of certainty according to the overall group composition of antioxidant defence enzymes. Our results indicate differences in the composition of antioxidant defence between controls and antipsychotic treated type I and type II patients with a possible negative feedback influence on the pathological process, which could provide a rationale for applying antioxidants during schizophrenic therapy. (C) 2009 Elsevier Ireland Ltd. All rights reserved.",
journal = "Psychiatry Research",
title = "Correlation analysis confirms differences in antioxidant defence in the blood of types I and II schizophrenic male patients treated with anti-psychotic medication",
number = "1",
volume = "178",
pages = "72",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1365"
}

Miljević, C. D., Nikolić-Kokić, A., Saičić, Z., Milosavljević, M., Blagojević, D., Lecić-Tosevski, D. M., Jones, D. R.,& Spasić, M.. (2010). Correlation analysis confirms differences in antioxidant defence in the blood of types I and II schizophrenic male patients treated with anti-psychotic medication. in Psychiatry Research, 178(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1365

Miljević CD, Nikolić-Kokić A, Saičić Z, Milosavljević M, Blagojević D, Lecić-Tosevski DM, Jones DR, Spasić M. Correlation analysis confirms differences in antioxidant defence in the blood of types I and II schizophrenic male patients treated with anti-psychotic medication. in Psychiatry Research. 2010;178(1):null-72.
https://hdl.handle.net/21.15107/rcub_ibiss_1365 .

Miljević, Cedo D, Nikolić-Kokić, Aleksandra, Saičić, Zorica, Milosavljević, Maja, Blagojević, Duško, Lecić-Tosevski, Dusica M, Jones, David R, Spasić, Mihajlo, "Correlation analysis confirms differences in antioxidant defence in the blood of types I and II schizophrenic male patients treated with anti-psychotic medication" in Psychiatry Research, 178, no. 1 (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1365 .

TY  - JOUR
AU  - Miljević, Cedo D
AU  - Nikolić, Milan R
AU  - Nikolić-Kokić, Aleksandra
AU  - Jones, David R
AU  - Niketić, Vesna P
AU  - Lecić-Tosevski, Dusica M
AU  - Spasić, Mihajlo
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1395
AB  - Objective: Despite clozapine's unique effectiveness in patients with schizophrenia, a number of adverse effects have been recognised including abnormalities in lipid and glucose metabolisms. A high clozapine level in red blood cells (RBCs) and disturbed anti-oxidant enzyme activities in blood from schizophrenic patients prompted us to investigate lipid status and anti-oxidant enzyme defence in the blood of chronic schizophrenic patients on long-term clozapine therapy. Methods: Plasma lipids, RBC anti-oxidant enzyme activities and haemoglobin (Hb) content were measured using established procedures in a group of eighteen chronically-medicated (average 630 days of therapy) schizophrenic patients receiving clozapine (average dose of 295 mg/day) and data were compared with those from a group of eighteen well-matched normal controls. Results: Significantly higher levels of plasma triglycerides (by 47%, p<0.01) and total cholesterol and phospholipids (by 8% and 11%, respectively p<0.05) in patients were found. CuZn-superoxide dismutase (SOD1) activity was markedly higher (by 35%, p<0.001) while selenium-dependent glutathione peroxidase (GSH-Px1) activity was markedly lower (by 41%, p<0.001) in patients. In addition, metHb and HbA1c levels in patients were significantly higher (by 58% and 25%. respectively p<0.001). SOD1 activity was negatively correlated (p<0.001) to GSH-Px1 activity in patients. Conclusions:The findings support the view that ongoing oxidative stress may be a mechanism by which clozapine induces some adverse effects that increase the risk of diabetes and metabolic syndrome. If valid, this would indicate that in parallel with long-term clozapine treatment, schizophrenic patients could be encouraged to make some lifestyle changes to limit the detrimental effects of the medication. (C) 2009 Elsevier Inc. All rights reserved.
T2  - Progress in Neuro-Psychopharmacology & Biological Psychiatry
T1  - Lipid status, anti-oxidant enzyme defence and haemoglobin content in the blood of long-term clozapine-treated schizophrenic patients
IS  - 2
VL  - 34
EP  - 307
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1395
ER  - 

@article{
author = "Miljević, Cedo D and Nikolić, Milan R and Nikolić-Kokić, Aleksandra and Jones, David R and Niketić, Vesna P and Lecić-Tosevski, Dusica M and Spasić, Mihajlo",
year = "2010",
abstract = "Objective: Despite clozapine's unique effectiveness in patients with schizophrenia, a number of adverse effects have been recognised including abnormalities in lipid and glucose metabolisms. A high clozapine level in red blood cells (RBCs) and disturbed anti-oxidant enzyme activities in blood from schizophrenic patients prompted us to investigate lipid status and anti-oxidant enzyme defence in the blood of chronic schizophrenic patients on long-term clozapine therapy. Methods: Plasma lipids, RBC anti-oxidant enzyme activities and haemoglobin (Hb) content were measured using established procedures in a group of eighteen chronically-medicated (average 630 days of therapy) schizophrenic patients receiving clozapine (average dose of 295 mg/day) and data were compared with those from a group of eighteen well-matched normal controls. Results: Significantly higher levels of plasma triglycerides (by 47%, p<0.01) and total cholesterol and phospholipids (by 8% and 11%, respectively p<0.05) in patients were found. CuZn-superoxide dismutase (SOD1) activity was markedly higher (by 35%, p<0.001) while selenium-dependent glutathione peroxidase (GSH-Px1) activity was markedly lower (by 41%, p<0.001) in patients. In addition, metHb and HbA1c levels in patients were significantly higher (by 58% and 25%. respectively p<0.001). SOD1 activity was negatively correlated (p<0.001) to GSH-Px1 activity in patients. Conclusions:The findings support the view that ongoing oxidative stress may be a mechanism by which clozapine induces some adverse effects that increase the risk of diabetes and metabolic syndrome. If valid, this would indicate that in parallel with long-term clozapine treatment, schizophrenic patients could be encouraged to make some lifestyle changes to limit the detrimental effects of the medication. (C) 2009 Elsevier Inc. All rights reserved.",
journal = "Progress in Neuro-Psychopharmacology & Biological Psychiatry",
title = "Lipid status, anti-oxidant enzyme defence and haemoglobin content in the blood of long-term clozapine-treated schizophrenic patients",
number = "2",
volume = "34",
pages = "307",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1395"
}

Miljević, C. D., Nikolić, M. R., Nikolić-Kokić, A., Jones, D. R., Niketić, V. P., Lecić-Tosevski, D. M.,& Spasić, M.. (2010). Lipid status, anti-oxidant enzyme defence and haemoglobin content in the blood of long-term clozapine-treated schizophrenic patients. in Progress in Neuro-Psychopharmacology & Biological Psychiatry, 34(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1395

Miljević CD, Nikolić MR, Nikolić-Kokić A, Jones DR, Niketić VP, Lecić-Tosevski DM, Spasić M. Lipid status, anti-oxidant enzyme defence and haemoglobin content in the blood of long-term clozapine-treated schizophrenic patients. in Progress in Neuro-Psychopharmacology & Biological Psychiatry. 2010;34(2):null-307.
https://hdl.handle.net/21.15107/rcub_ibiss_1395 .

Miljević, Cedo D, Nikolić, Milan R, Nikolić-Kokić, Aleksandra, Jones, David R, Niketić, Vesna P, Lecić-Tosevski, Dusica M, Spasić, Mihajlo, "Lipid status, anti-oxidant enzyme defence and haemoglobin content in the blood of long-term clozapine-treated schizophrenic patients" in Progress in Neuro-Psychopharmacology & Biological Psychiatry, 34, no. 2 (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1395 .