@conference{
author = "Perić, Mina and Nikolić, Ljiljana and Anđus, Pavle R. and Bataveljić, Danijela",
year = "2020",
abstract = "Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease affecting lower
and upper motor neurons, leading to muscle atrophy, paralysis and death. Although
ALS is characterized by motoneuron degeneration, non-neuronal cells such as
oligodendrocytes play an essential role in disease onset and progression. The aim
of this study was to investigate functional properties of oligodendrocytes in the ALS
with particular focus on their role in the regulation of extracellular K+ through inwardly
rectifying potassium channel Kir4.1 abundantly expressed in these glial cells. We
employed immunolabeling of Kir4.1, and oligodendrocyte-specific marker CNPase,
to examine the expression of Kir4.1 in oligodendrocytes of the lumbar spinal cord of
SOD1G93A rat model of ALS. Furthermore, whole-cell patch-clamp recordings were
performed on primary oligodendrocyte cultures from ALS and control rats to
investigate functional properties of these cells. Our data show reduced expression
of Kir4.1 in the oligodendrocytes in the lumbar spinal cord of SOD1G93A rat compared
to control. Moreover, we found elevated number of dysmorphic oligodendrocytes in
the ALS spinal cord, indicative of a degenerative phenotype. Oligodendrocytes
isolated from SOD1G93A spinal cord display similar processes ramification as the
control, however expressing a lower level of Kir4.1. Electrophysiological examination
of cultured SOD1G93A oligodendrocytes revealed compromised membrane
properties and diminished inward currents in comparison to control. In addition, the
Ba2+-sensitive Kir current is decreased in ALS oligodendrocytes. Altogether, our
findings provide the evidence of impaired Kir4.1 expression and function in
SOD1G93A spinal cord oligodendrocytes suggesting a critical role of oligodendrocyte
Kir4.1 channel in ALS pathophysiology.",
publisher = "Querétaro, México: Instituto de neurobiologia",
journal = "Proceedings: 3rd Symposium on Physiology and pathology of neuroglia; 2020 Noc 24-25; Virtual",
title = "Dysfunction of oligodendrocyte inwardly rectifying potassium channel in a rat model of amyotrophic lateral sclerosis",
pages = "75",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5499"
}