TY  - JOUR
AU  - Drvenica, Ivana T.
AU  - Bukara, Katarina M.
AU  - Ilić, Vesna Lj.
AU  - Mišić, Danijela
AU  - Vasić, Borislav Z.
AU  - Gajić, Radoš B.
AU  - Đorđević, Verica B.
AU  - Veljović, Đorđe N.
AU  - Belić, Aleksandar
AU  - Bugarski, Branko M.
PY  - 2016
UR  - http://doi.wiley.com/10.1002/btpr.2304
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2541
AB  - The present study investigated preparation of bovine and porcine erythrocyte membranes from slaughterhouse blood as bio-derived materials for delivery of dexamethasone-sodium phosphate (DexP). The obtained biomembranes, i.e., ghosts were characterized in vitro in terms of morphological properties, loading parameters, and release behavior. For the last two, an UHPLC/-HESI-MS/MS based analytical procedure for absolute drug identification and quantification was developed. The results revealed that loading of DexP into both type of ghosts was directly proportional to the increase of drug concentration in the incubation medium, while incubation at 37°C had statistically significant effect on loaded amount of DexP (P<0.05). The encapsulation efficiency was about fivefold higher in porcine compared to bovine ghosts. Insight into ghosts' surface morphology by field emission-scanning electron microscopy and atomic force microscopy confirmed that besides inevitable effects of osmosis, DexP inclusion itself had no observable additional effect on the morphology of the ghosts carriers. DexP release profiles were dependent on erythrocyte ghost type and amount of residual hemoglobin. However, sustained DexP release was achieved and shown over 3 days from porcine ghosts and 5 days from bovine erythrocyte ghosts.
T2  - Biotechnology Progress
T1  - Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate
IS  - 4
VL  - 32
DO  - 10.1002/btpr.2304
SP  - 1046
EP  - 1055
ER  - 

@article{
author = "Drvenica, Ivana T. and Bukara, Katarina M. and Ilić, Vesna Lj. and Mišić, Danijela and Vasić, Borislav Z. and Gajić, Radoš B. and Đorđević, Verica B. and Veljović, Đorđe N. and Belić, Aleksandar and Bugarski, Branko M.",
year = "2016",
abstract = "The present study investigated preparation of bovine and porcine erythrocyte membranes from slaughterhouse blood as bio-derived materials for delivery of dexamethasone-sodium phosphate (DexP). The obtained biomembranes, i.e., ghosts were characterized in vitro in terms of morphological properties, loading parameters, and release behavior. For the last two, an UHPLC/-HESI-MS/MS based analytical procedure for absolute drug identification and quantification was developed. The results revealed that loading of DexP into both type of ghosts was directly proportional to the increase of drug concentration in the incubation medium, while incubation at 37°C had statistically significant effect on loaded amount of DexP (P<0.05). The encapsulation efficiency was about fivefold higher in porcine compared to bovine ghosts. Insight into ghosts' surface morphology by field emission-scanning electron microscopy and atomic force microscopy confirmed that besides inevitable effects of osmosis, DexP inclusion itself had no observable additional effect on the morphology of the ghosts carriers. DexP release profiles were dependent on erythrocyte ghost type and amount of residual hemoglobin. However, sustained DexP release was achieved and shown over 3 days from porcine ghosts and 5 days from bovine erythrocyte ghosts.",
journal = "Biotechnology Progress",
title = "Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate",
number = "4",
volume = "32",
doi = "10.1002/btpr.2304",
pages = "1046-1055"
}

Drvenica, I. T., Bukara, K. M., Ilić, V. Lj., Mišić, D., Vasić, B. Z., Gajić, R. B., Đorđević, V. B., Veljović, Đ. N., Belić, A.,& Bugarski, B. M.. (2016). Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate. in Biotechnology Progress, 32(4), 1046-1055.
https://doi.org/10.1002/btpr.2304

Drvenica IT, Bukara KM, Ilić VL, Mišić D, Vasić BZ, Gajić RB, Đorđević VB, Veljović ĐN, Belić A, Bugarski BM. Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate. in Biotechnology Progress. 2016;32(4):1046-1055.
doi:10.1002/btpr.2304 .

Drvenica, Ivana T., Bukara, Katarina M., Ilić, Vesna Lj., Mišić, Danijela, Vasić, Borislav Z., Gajić, Radoš B., Đorđević, Verica B., Veljović, Đorđe N., Belić, Aleksandar, Bugarski, Branko M., "Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate" in Biotechnology Progress, 32, no. 4 (2016):1046-1055,
https://doi.org/10.1002/btpr.2304 . .