@conference{
author = "Habboubi, Baha and Dias, Maria Inês and Pinela, José and Ćirić, Ana and Soković, Marina and Ichrak, Charfi and Ferreira, Isabel C. F. R. and Barros, Lillian",
year = "2021",
abstract = "Bixa orellana L. is worldwide known as a source of bixin, a carotenoid compound with high colorant
capacity [1]. The growing tendency in the food industry to use new and safer colorant compounds with
higher stability coupled to the high demand of consumers concern, became of the upmost importance.
Moreover, sustainable extraction technologies coupled with greener solvents are increasingly required to
maximize the recovery of high added value compounds [2]. The present work intended to deepen the study
of the nutritional and chemical profile and bioactive potential of B. orellana seeds. Furthermore, to
maximize the extraction of bixin from seeds ultrasound-assisted technologies combined with RSM were
used. As an externality of this work, the pods of this plant (bio-residues resulting from the processing of
seeds) were also studied, in relation to its bioactive properties and profile in phenolic compounds. The
most abundant macronutrients found in seeds were carbohydrates, followed by fat, proteins, and ash.
Sucrose and trehalose were the only sugars found; and malic acid and α-tocopherol were the main
organic acid and tocopherols present, respectively. Monounsaturated fatty acids (eicosenoic acid) were
found in higher amounts. Pods hydroethanolic extract presented lower IC50 values for antioxidant activity;
while seeds sample revealed lower IC50 values for Δt = 60 min and Δt = 120 min for OxHLIA assay, and
lower GI50 values for cytotoxic and hepatotoxic assays. Both samples presented lower MIC, MBC, and
MFC in comparison to the two positive controls, against all bacterial and fungal strains. Pods presented
the highest amounts of phenolic compounds (due to protocatechuic acid). The model developed for the
extraction process was validated, with optimal processing conditions (sonication - 348 W, 6 min, 79 %
(v/v) ethanol), being possible to obtain 27.1 mg of bixin per g of extract.
Overall, this study allowed to present innovative results in relation to the nutritional, chemical and
bioactive properties of the seeds, and as an externality the great potential of the pods regarding its
biological activity. Considering the good results obtained in the optimization procedure, it is worth
mentioning the sustainable way in which this extract can be obtained.",
publisher = "Bragança, Portugal: Instituto Politécnico de Bragança (IPB)",
journal = "1st Natural products application: Health, Cosmetic and Food: book of abstracts",
title = "Bixa orellana L. pods and seeds: nutritional and chemical characterization, bioactivity studies, and development of a carotenoid-based food colorant",
pages = "179",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4379"
}
