TY  - JOUR
AU  - Miljević, Cedo D
AU  - Nikolić, Milan R
AU  - Nikolić-Kokić, Aleksandra
AU  - Jones, David R
AU  - Niketić, Vesna P
AU  - Lecić-Tosevski, Dusica M
AU  - Spasić, Mihajlo
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1395
AB  - Objective: Despite clozapine's unique effectiveness in patients with schizophrenia, a number of adverse effects have been recognised including abnormalities in lipid and glucose metabolisms. A high clozapine level in red blood cells (RBCs) and disturbed anti-oxidant enzyme activities in blood from schizophrenic patients prompted us to investigate lipid status and anti-oxidant enzyme defence in the blood of chronic schizophrenic patients on long-term clozapine therapy. Methods: Plasma lipids, RBC anti-oxidant enzyme activities and haemoglobin (Hb) content were measured using established procedures in a group of eighteen chronically-medicated (average 630 days of therapy) schizophrenic patients receiving clozapine (average dose of 295 mg/day) and data were compared with those from a group of eighteen well-matched normal controls. Results: Significantly higher levels of plasma triglycerides (by 47%, p<0.01) and total cholesterol and phospholipids (by 8% and 11%, respectively p<0.05) in patients were found. CuZn-superoxide dismutase (SOD1) activity was markedly higher (by 35%, p<0.001) while selenium-dependent glutathione peroxidase (GSH-Px1) activity was markedly lower (by 41%, p<0.001) in patients. In addition, metHb and HbA1c levels in patients were significantly higher (by 58% and 25%. respectively p<0.001). SOD1 activity was negatively correlated (p<0.001) to GSH-Px1 activity in patients. Conclusions:The findings support the view that ongoing oxidative stress may be a mechanism by which clozapine induces some adverse effects that increase the risk of diabetes and metabolic syndrome. If valid, this would indicate that in parallel with long-term clozapine treatment, schizophrenic patients could be encouraged to make some lifestyle changes to limit the detrimental effects of the medication. (C) 2009 Elsevier Inc. All rights reserved.
T2  - Progress in Neuro-Psychopharmacology & Biological Psychiatry
T1  - Lipid status, anti-oxidant enzyme defence and haemoglobin content in the blood of long-term clozapine-treated schizophrenic patients
IS  - 2
VL  - 34
EP  - 307
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1395
ER  - 

@article{
author = "Miljević, Cedo D and Nikolić, Milan R and Nikolić-Kokić, Aleksandra and Jones, David R and Niketić, Vesna P and Lecić-Tosevski, Dusica M and Spasić, Mihajlo",
year = "2010",
abstract = "Objective: Despite clozapine's unique effectiveness in patients with schizophrenia, a number of adverse effects have been recognised including abnormalities in lipid and glucose metabolisms. A high clozapine level in red blood cells (RBCs) and disturbed anti-oxidant enzyme activities in blood from schizophrenic patients prompted us to investigate lipid status and anti-oxidant enzyme defence in the blood of chronic schizophrenic patients on long-term clozapine therapy. Methods: Plasma lipids, RBC anti-oxidant enzyme activities and haemoglobin (Hb) content were measured using established procedures in a group of eighteen chronically-medicated (average 630 days of therapy) schizophrenic patients receiving clozapine (average dose of 295 mg/day) and data were compared with those from a group of eighteen well-matched normal controls. Results: Significantly higher levels of plasma triglycerides (by 47%, p<0.01) and total cholesterol and phospholipids (by 8% and 11%, respectively p<0.05) in patients were found. CuZn-superoxide dismutase (SOD1) activity was markedly higher (by 35%, p<0.001) while selenium-dependent glutathione peroxidase (GSH-Px1) activity was markedly lower (by 41%, p<0.001) in patients. In addition, metHb and HbA1c levels in patients were significantly higher (by 58% and 25%. respectively p<0.001). SOD1 activity was negatively correlated (p<0.001) to GSH-Px1 activity in patients. Conclusions:The findings support the view that ongoing oxidative stress may be a mechanism by which clozapine induces some adverse effects that increase the risk of diabetes and metabolic syndrome. If valid, this would indicate that in parallel with long-term clozapine treatment, schizophrenic patients could be encouraged to make some lifestyle changes to limit the detrimental effects of the medication. (C) 2009 Elsevier Inc. All rights reserved.",
journal = "Progress in Neuro-Psychopharmacology & Biological Psychiatry",
title = "Lipid status, anti-oxidant enzyme defence and haemoglobin content in the blood of long-term clozapine-treated schizophrenic patients",
number = "2",
volume = "34",
pages = "307",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1395"
}

Miljević, C. D., Nikolić, M. R., Nikolić-Kokić, A., Jones, D. R., Niketić, V. P., Lecić-Tosevski, D. M.,& Spasić, M.. (2010). Lipid status, anti-oxidant enzyme defence and haemoglobin content in the blood of long-term clozapine-treated schizophrenic patients. in Progress in Neuro-Psychopharmacology & Biological Psychiatry, 34(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1395

Miljević CD, Nikolić MR, Nikolić-Kokić A, Jones DR, Niketić VP, Lecić-Tosevski DM, Spasić M. Lipid status, anti-oxidant enzyme defence and haemoglobin content in the blood of long-term clozapine-treated schizophrenic patients. in Progress in Neuro-Psychopharmacology & Biological Psychiatry. 2010;34(2):null-307.
https://hdl.handle.net/21.15107/rcub_ibiss_1395 .

Miljević, Cedo D, Nikolić, Milan R, Nikolić-Kokić, Aleksandra, Jones, David R, Niketić, Vesna P, Lecić-Tosevski, Dusica M, Spasić, Mihajlo, "Lipid status, anti-oxidant enzyme defence and haemoglobin content in the blood of long-term clozapine-treated schizophrenic patients" in Progress in Neuro-Psychopharmacology & Biological Psychiatry, 34, no. 2 (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1395 .

TY  - JOUR
AU  - Nikolić-Kokić, Aleksandra
AU  - Stević, Zorica D
AU  - Stojanović, Srđan D.
AU  - Blagojević, Duško
AU  - Jones, David R
AU  - Pavlović, Sanja
AU  - Niketić, Vesna P
AU  - Apostolski, Slobodan A
AU  - Spasić, Mihajlo
PY  - 2005
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1689
AB  - Recent findings indicate that nitric oxide (NOcenter dot) over-production might be an important factor in the pathogenesis of sporadic amyotrophic lateral sclerosis (SALS). We measured significantly higher concentrations of uric acid and thiol group-containing molecules (R-SH groups) in the cerebrospinal fluid (CSF) from SALS patients compared to controls. The above factors, together with a slightly increased free iron concentration found in the CSF, favour conditions necessary for the formation of the dinitrosyl iron complex, capable of NOcenter dot bio-transformation. Thus, we performed ex vivo saturation of CSF ( from both SALS patients and controls) with NOcenter dot. A decrease in the level of R - SH was found. This was more pronounced in the CSF from SALS patients. In the CSF from SALS patients the production of nitrite and hydroxylamine was greater than that observed in the CSF from controls. Moreover, we also found increased Cu, Zn-SOD activity in the CSF from SALS patients ( when compared to control subjects) but no activity corresponding to Mn-SOD in any CSF samples. As Cu, Zn-SOD can react with nitroxyl forming NOcenter dot, the conditions for a closed, but continuous, loop of NOcenter dot biotransformation are present in the CSF of ALS patients.
T2  - Redox Report
T1  - Biotransformation of nitric oxide in the cerebrospinal fluid of amyotrophic lateral sclerosis patients
IS  - 5
VL  - 10
EP  - 270
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1689
ER  - 

@article{
author = "Nikolić-Kokić, Aleksandra and Stević, Zorica D and Stojanović, Srđan D. and Blagojević, Duško and Jones, David R and Pavlović, Sanja and Niketić, Vesna P and Apostolski, Slobodan A and Spasić, Mihajlo",
year = "2005",
abstract = "Recent findings indicate that nitric oxide (NOcenter dot) over-production might be an important factor in the pathogenesis of sporadic amyotrophic lateral sclerosis (SALS). We measured significantly higher concentrations of uric acid and thiol group-containing molecules (R-SH groups) in the cerebrospinal fluid (CSF) from SALS patients compared to controls. The above factors, together with a slightly increased free iron concentration found in the CSF, favour conditions necessary for the formation of the dinitrosyl iron complex, capable of NOcenter dot bio-transformation. Thus, we performed ex vivo saturation of CSF ( from both SALS patients and controls) with NOcenter dot. A decrease in the level of R - SH was found. This was more pronounced in the CSF from SALS patients. In the CSF from SALS patients the production of nitrite and hydroxylamine was greater than that observed in the CSF from controls. Moreover, we also found increased Cu, Zn-SOD activity in the CSF from SALS patients ( when compared to control subjects) but no activity corresponding to Mn-SOD in any CSF samples. As Cu, Zn-SOD can react with nitroxyl forming NOcenter dot, the conditions for a closed, but continuous, loop of NOcenter dot biotransformation are present in the CSF of ALS patients.",
journal = "Redox Report",
title = "Biotransformation of nitric oxide in the cerebrospinal fluid of amyotrophic lateral sclerosis patients",
number = "5",
volume = "10",
pages = "270",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1689"
}

Nikolić-Kokić, A., Stević, Z. D., Stojanović, S. D., Blagojević, D., Jones, D. R., Pavlović, S., Niketić, V. P., Apostolski, S. A.,& Spasić, M.. (2005). Biotransformation of nitric oxide in the cerebrospinal fluid of amyotrophic lateral sclerosis patients. in Redox Report, 10(5).
https://hdl.handle.net/21.15107/rcub_ibiss_1689

Nikolić-Kokić A, Stević ZD, Stojanović SD, Blagojević D, Jones DR, Pavlović S, Niketić VP, Apostolski SA, Spasić M. Biotransformation of nitric oxide in the cerebrospinal fluid of amyotrophic lateral sclerosis patients. in Redox Report. 2005;10(5):null-270.
https://hdl.handle.net/21.15107/rcub_ibiss_1689 .

Nikolić-Kokić, Aleksandra, Stević, Zorica D, Stojanović, Srđan D., Blagojević, Duško, Jones, David R, Pavlović, Sanja, Niketić, Vesna P, Apostolski, Slobodan A, Spasić, Mihajlo, "Biotransformation of nitric oxide in the cerebrospinal fluid of amyotrophic lateral sclerosis patients" in Redox Report, 10, no. 5 (2005),
https://hdl.handle.net/21.15107/rcub_ibiss_1689 .

TY  - CONF
AU  - Nikolić, Aleksandra L.
AU  - Blagojević, Duško
AU  - Stević, Zorica D
AU  - Niketić, Vesna P
AU  - Saičić, Zorica
AU  - Spasić, Mihajlo
PY  - 2002
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1804
C3  - Free Radical Biology and Medicine
T1  - Activities of antioxidative defence enzymes in the blood of patients with amyotrophic lateral sclerosis - Base for controled trial of antioxidants combination
IS  - null
VL  - 33
EP  - S126
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1804
ER  - 

@conference{
author = "Nikolić, Aleksandra L. and Blagojević, Duško and Stević, Zorica D and Niketić, Vesna P and Saičić, Zorica and Spasić, Mihajlo",
year = "2002",
journal = "Free Radical Biology and Medicine",
title = "Activities of antioxidative defence enzymes in the blood of patients with amyotrophic lateral sclerosis - Base for controled trial of antioxidants combination",
number = "null",
volume = "33",
pages = "S126",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1804"
}

Nikolić, A. L., Blagojević, D., Stević, Z. D., Niketić, V. P., Saičić, Z.,& Spasić, M.. (2002). Activities of antioxidative defence enzymes in the blood of patients with amyotrophic lateral sclerosis - Base for controled trial of antioxidants combination. in Free Radical Biology and Medicine, 33(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1804

Nikolić AL, Blagojević D, Stević ZD, Niketić VP, Saičić Z, Spasić M. Activities of antioxidative defence enzymes in the blood of patients with amyotrophic lateral sclerosis - Base for controled trial of antioxidants combination. in Free Radical Biology and Medicine. 2002;33(null):null-S126.
https://hdl.handle.net/21.15107/rcub_ibiss_1804 .

Nikolić, Aleksandra L., Blagojević, Duško, Stević, Zorica D, Niketić, Vesna P, Saičić, Zorica, Spasić, Mihajlo, "Activities of antioxidative defence enzymes in the blood of patients with amyotrophic lateral sclerosis - Base for controled trial of antioxidants combination" in Free Radical Biology and Medicine, 33, no. null (2002),
https://hdl.handle.net/21.15107/rcub_ibiss_1804 .