TY  - JOUR
AU  - Marković, Snezana D
AU  - Zizić, Jovana B
AU  - Obradović, Ana D
AU  - Ognjanović, Branka I
AU  - Stajn, Andras S
AU  - Saičić, Zorica
AU  - Spasić, Mihajlo
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1287
AB  - Stimulated erythropoiesis and reticulocytosis can be induced by daily bleeding, or by phenylhydrazine (PHZ) treatment. We compared the in vivo effects of PHZ and bleeding treatment on haematological, energy and redox status parameters in red blood cells (RBC) of rats. The results showed that all followed haematological parameters were significantly lower in bleeding, compared to PHZ-treated rats. PHZ induced even 2.58-fold higher reticulocytosis as compared to bleeding treatment. Although PHZ induced higher reticulocytosis, respiration intensity and energy production was lower than in bleeding-induced reticulocytes. These alterations were the consequence of increased superoxide anion and peroxynitrite concentrations in PHZ-treated rats. Bleeding treatment resulted in increased activity of an antioxidative enzyme, superoxide dismutase. In conclusion, differences in these two experimental models for reticulocytosis may be used as tools for appropriate pharmacological testing of redox-active substances considering energy and redox processes, as well as apoptosis pathways.
T2  - Acta Biologica Hungarica
T1  - Energy Production and Redox Status of Rat Red Blood Cells After Reticulocytosis Induced By Various Treatments
IS  - 2
VL  - 62
EP  - 132
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1287
ER  - 

@article{
author = "Marković, Snezana D and Zizić, Jovana B and Obradović, Ana D and Ognjanović, Branka I and Stajn, Andras S and Saičić, Zorica and Spasić, Mihajlo",
year = "2011",
abstract = "Stimulated erythropoiesis and reticulocytosis can be induced by daily bleeding, or by phenylhydrazine (PHZ) treatment. We compared the in vivo effects of PHZ and bleeding treatment on haematological, energy and redox status parameters in red blood cells (RBC) of rats. The results showed that all followed haematological parameters were significantly lower in bleeding, compared to PHZ-treated rats. PHZ induced even 2.58-fold higher reticulocytosis as compared to bleeding treatment. Although PHZ induced higher reticulocytosis, respiration intensity and energy production was lower than in bleeding-induced reticulocytes. These alterations were the consequence of increased superoxide anion and peroxynitrite concentrations in PHZ-treated rats. Bleeding treatment resulted in increased activity of an antioxidative enzyme, superoxide dismutase. In conclusion, differences in these two experimental models for reticulocytosis may be used as tools for appropriate pharmacological testing of redox-active substances considering energy and redox processes, as well as apoptosis pathways.",
journal = "Acta Biologica Hungarica",
title = "Energy Production and Redox Status of Rat Red Blood Cells After Reticulocytosis Induced By Various Treatments",
number = "2",
volume = "62",
pages = "132",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1287"
}

Marković, S. D., Zizić, J. B., Obradović, A. D., Ognjanović, B. I., Stajn, A. S., Saičić, Z.,& Spasić, M.. (2011). Energy Production and Redox Status of Rat Red Blood Cells After Reticulocytosis Induced By Various Treatments. in Acta Biologica Hungarica, 62(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1287

Marković SD, Zizić JB, Obradović AD, Ognjanović BI, Stajn AS, Saičić Z, Spasić M. Energy Production and Redox Status of Rat Red Blood Cells After Reticulocytosis Induced By Various Treatments. in Acta Biologica Hungarica. 2011;62(2):null-132.
https://hdl.handle.net/21.15107/rcub_ibiss_1287 .

Marković, Snezana D, Zizić, Jovana B, Obradović, Ana D, Ognjanović, Branka I, Stajn, Andras S, Saičić, Zorica, Spasić, Mihajlo, "Energy Production and Redox Status of Rat Red Blood Cells After Reticulocytosis Induced By Various Treatments" in Acta Biologica Hungarica, 62, no. 2 (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1287 .

TY  - JOUR
AU  - Đorđević, Natasa Z
AU  - Babić, Goran M
AU  - Marković, Snezana D
AU  - Ognjanović, Branka I
AU  - Stajn, Andras S
AU  - Saičić, Zorica
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1394
AB  - In the present study, we evaluated changes of both oxidative stress marker concentrations in erythrocytes and values of blood pressure, as well as their relation during short-term estradiol therapy in preeclampsia. Serum estradiol concentrations were also recorded. The results of this study showed significant decrease of mean arterial pressure (MAP) values during estradiol therapy, whereas there was no significant change in serum estradiol concentrations. Decreased concentrations of superoxide anion (O(2)(center dot-)), hydrogen peroxide (H(2)O(2)), nitrite (NO(2)(-)), peroxynitrite (ONOO(-)) and lipid peroxide (LPO) were found during estradiol therapy in erythrocytes. No changes were found in the activity of gluthatione-S-transferase (GST). The decrease of MAP values was positively correlated with the reduction of concentrations of O(2)(center dot-), H(2)O(2), NO(2)(-) and ONOO(-) in erythrocytes during estradiol therapy. The obtained results suggest that short-term intramuscular administration of estradiol shows antioxidative effects in erythrocytes and reduces blood pressure in preeclampsia. (C) 2009 Elsevier Inc. All rights reserved.
T2  - Reproductive Toxicology
T1  - The antioxidative effect of estradiol therapy on erythrocytes in women with preeclampsia
IS  - 2
VL  - 29
EP  - 236
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1394
ER  - 

@article{
author = "Đorđević, Natasa Z and Babić, Goran M and Marković, Snezana D and Ognjanović, Branka I and Stajn, Andras S and Saičić, Zorica",
year = "2010",
abstract = "In the present study, we evaluated changes of both oxidative stress marker concentrations in erythrocytes and values of blood pressure, as well as their relation during short-term estradiol therapy in preeclampsia. Serum estradiol concentrations were also recorded. The results of this study showed significant decrease of mean arterial pressure (MAP) values during estradiol therapy, whereas there was no significant change in serum estradiol concentrations. Decreased concentrations of superoxide anion (O(2)(center dot-)), hydrogen peroxide (H(2)O(2)), nitrite (NO(2)(-)), peroxynitrite (ONOO(-)) and lipid peroxide (LPO) were found during estradiol therapy in erythrocytes. No changes were found in the activity of gluthatione-S-transferase (GST). The decrease of MAP values was positively correlated with the reduction of concentrations of O(2)(center dot-), H(2)O(2), NO(2)(-) and ONOO(-) in erythrocytes during estradiol therapy. The obtained results suggest that short-term intramuscular administration of estradiol shows antioxidative effects in erythrocytes and reduces blood pressure in preeclampsia. (C) 2009 Elsevier Inc. All rights reserved.",
journal = "Reproductive Toxicology",
title = "The antioxidative effect of estradiol therapy on erythrocytes in women with preeclampsia",
number = "2",
volume = "29",
pages = "236",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1394"
}

Đorđević, N. Z., Babić, G. M., Marković, S. D., Ognjanović, B. I., Stajn, A. S.,& Saičić, Z.. (2010). The antioxidative effect of estradiol therapy on erythrocytes in women with preeclampsia. in Reproductive Toxicology, 29(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1394

Đorđević NZ, Babić GM, Marković SD, Ognjanović BI, Stajn AS, Saičić Z. The antioxidative effect of estradiol therapy on erythrocytes in women with preeclampsia. in Reproductive Toxicology. 2010;29(2):null-236.
https://hdl.handle.net/21.15107/rcub_ibiss_1394 .

Đorđević, Natasa Z, Babić, Goran M, Marković, Snezana D, Ognjanović, Branka I, Stajn, Andras S, Saičić, Zorica, "The antioxidative effect of estradiol therapy on erythrocytes in women with preeclampsia" in Reproductive Toxicology, 29, no. 2 (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1394 .

TY  - JOUR
AU  - Ognjanović, Branka I
AU  - Marković, Snezana D
AU  - Đorđević, Natasa Z
AU  - Trbojević, Ivana S
AU  - Stajn, Andras S
AU  - Saičić, Zorica
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1393
AB  - The aim of this study was to investigate the protective role of coenzyme Q(10) (CoQ(10), 20 mg/kg) and Vitamin E (Vit E, 20IU/kg) alone or in combination against cadmium (Cd, 0.4 mg/kg) induced lipid peroxidation and changes in antioxidant defense system in the rat testes. The obtained results showed that Cd increased lipid peroxidation in the testes, suggesting that Cd-induced oxidative stress, while CoQ(10) and Vit E treatment reversed this change to control values. Acute intoxication with Cd was followed by significantly decreased activity of antioxidant enzymes (SOD, CAT, GSH-Px, GR and GST). Vitamins C and E concentrations also significantly declined in Cd-exposed rat testes. Treatment with CoQ(10), and Vit E reversed Cd-induced alterations of antioxidant defense system and significantly prevented Cd-induced testes damage. These results suggest that both CoQic, and Vit E function as a potent antioxidant in protection of rats testes against the oxidative stress induced by Cd. (C) 2009 Elsevier Inc. All rights reserved.
T2  - Reproductive Toxicology
T1  - Cadmium-induced lipid peroxidation and changes in antioxidant defense system in the rat testes: Protective role of coenzyme Q(10) and Vitamin E
IS  - 2
VL  - 29
EP  - 197
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1393
ER  - 

@article{
author = "Ognjanović, Branka I and Marković, Snezana D and Đorđević, Natasa Z and Trbojević, Ivana S and Stajn, Andras S and Saičić, Zorica",
year = "2010",
abstract = "The aim of this study was to investigate the protective role of coenzyme Q(10) (CoQ(10), 20 mg/kg) and Vitamin E (Vit E, 20IU/kg) alone or in combination against cadmium (Cd, 0.4 mg/kg) induced lipid peroxidation and changes in antioxidant defense system in the rat testes. The obtained results showed that Cd increased lipid peroxidation in the testes, suggesting that Cd-induced oxidative stress, while CoQ(10) and Vit E treatment reversed this change to control values. Acute intoxication with Cd was followed by significantly decreased activity of antioxidant enzymes (SOD, CAT, GSH-Px, GR and GST). Vitamins C and E concentrations also significantly declined in Cd-exposed rat testes. Treatment with CoQ(10), and Vit E reversed Cd-induced alterations of antioxidant defense system and significantly prevented Cd-induced testes damage. These results suggest that both CoQic, and Vit E function as a potent antioxidant in protection of rats testes against the oxidative stress induced by Cd. (C) 2009 Elsevier Inc. All rights reserved.",
journal = "Reproductive Toxicology",
title = "Cadmium-induced lipid peroxidation and changes in antioxidant defense system in the rat testes: Protective role of coenzyme Q(10) and Vitamin E",
number = "2",
volume = "29",
pages = "197",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1393"
}

Ognjanović, B. I., Marković, S. D., Đorđević, N. Z., Trbojević, I. S., Stajn, A. S.,& Saičić, Z.. (2010). Cadmium-induced lipid peroxidation and changes in antioxidant defense system in the rat testes: Protective role of coenzyme Q(10) and Vitamin E. in Reproductive Toxicology, 29(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1393

Ognjanović BI, Marković SD, Đorđević NZ, Trbojević IS, Stajn AS, Saičić Z. Cadmium-induced lipid peroxidation and changes in antioxidant defense system in the rat testes: Protective role of coenzyme Q(10) and Vitamin E. in Reproductive Toxicology. 2010;29(2):null-197.
https://hdl.handle.net/21.15107/rcub_ibiss_1393 .

Ognjanović, Branka I, Marković, Snezana D, Đorđević, Natasa Z, Trbojević, Ivana S, Stajn, Andras S, Saičić, Zorica, "Cadmium-induced lipid peroxidation and changes in antioxidant defense system in the rat testes: Protective role of coenzyme Q(10) and Vitamin E" in Reproductive Toxicology, 29, no. 2 (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1393 .

TY  - JOUR
AU  - Ognjanović, Branka I
AU  - Marković, Snezana D
AU  - Pavlović, Slađan
AU  - Žikić, Radoslav V.
AU  - Stajn, Andras S
AU  - Saičić, Zorica
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1562
UR  - http://www.biomed.cas.cz/physiolres/pdf/57/57_403.pdf
AB  - The effects of selenium ( Se) on antioxidant defense system in liver and kidneys of rats with cadmium (Cd)-induced toxicity were examined. Cd exposure (15 mg Cd/kg b.m./day as CdCl2 for 4 weeks) resulted in increased lipid peroxidation ( LP) in both organs (p < 0.005 and p < 0.01). Vitamin C (Vit C) was decreased in the liver ( p < 0.005), whereas vitamin E ( Vit E) was increased in the liver and kidneys ( p < 0.005 and p < 0.05) of Cd-exposed animals. Superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities were decreased in both tissues ( p < 0.05 and p < 0.005), whereas catalase (CAT) activity was decreased only in liver ( p < 0.005). Glutathione S-transferase (GST) increased in both tissues ( p < 0.005 and p<0.01). Treatment with Se (0.5 mg Se/kg b.m./day as Na2SeO3 for 4 weeks) significantly increased liver and kidneys SOD and GSH-Px activities ( p < 0.05 to p < 0.005), as well as CAT and GST activities only in the liver ( p < 0.01). In animals exposed to Se, both the concentrations of Vit C ( p < 0.01) and Vit E (p < 0.005) were increased in both tissues. Co-treatment with Se resulted in reversal of oxidative stress with significant decline in analyzed tissues Cd burden. Our results show that Se may ameliorate Cd-induced oxidative stress by decreasing LP and altering antioxidant defense system in rat liver and kidneys and that Se demonstrates the protective effect from cadmium-induced oxidative damage.
T2  - Physiological Research
T1  - Effect of chronic cadmium exposure on antioxidant defense system in some tissues of rats: Protective effect of selenium
IS  - 3
VL  - 57
EP  - 411
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1562
ER  - 

@article{
author = "Ognjanović, Branka I and Marković, Snezana D and Pavlović, Slađan and Žikić, Radoslav V. and Stajn, Andras S and Saičić, Zorica",
year = "2008",
abstract = "The effects of selenium ( Se) on antioxidant defense system in liver and kidneys of rats with cadmium (Cd)-induced toxicity were examined. Cd exposure (15 mg Cd/kg b.m./day as CdCl2 for 4 weeks) resulted in increased lipid peroxidation ( LP) in both organs (p < 0.005 and p < 0.01). Vitamin C (Vit C) was decreased in the liver ( p < 0.005), whereas vitamin E ( Vit E) was increased in the liver and kidneys ( p < 0.005 and p < 0.05) of Cd-exposed animals. Superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities were decreased in both tissues ( p < 0.05 and p < 0.005), whereas catalase (CAT) activity was decreased only in liver ( p < 0.005). Glutathione S-transferase (GST) increased in both tissues ( p < 0.005 and p<0.01). Treatment with Se (0.5 mg Se/kg b.m./day as Na2SeO3 for 4 weeks) significantly increased liver and kidneys SOD and GSH-Px activities ( p < 0.05 to p < 0.005), as well as CAT and GST activities only in the liver ( p < 0.01). In animals exposed to Se, both the concentrations of Vit C ( p < 0.01) and Vit E (p < 0.005) were increased in both tissues. Co-treatment with Se resulted in reversal of oxidative stress with significant decline in analyzed tissues Cd burden. Our results show that Se may ameliorate Cd-induced oxidative stress by decreasing LP and altering antioxidant defense system in rat liver and kidneys and that Se demonstrates the protective effect from cadmium-induced oxidative damage.",
journal = "Physiological Research",
title = "Effect of chronic cadmium exposure on antioxidant defense system in some tissues of rats: Protective effect of selenium",
number = "3",
volume = "57",
pages = "411",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1562"
}

Ognjanović, B. I., Marković, S. D., Pavlović, S., Žikić, R. V., Stajn, A. S.,& Saičić, Z.. (2008). Effect of chronic cadmium exposure on antioxidant defense system in some tissues of rats: Protective effect of selenium. in Physiological Research, 57(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1562

Ognjanović BI, Marković SD, Pavlović S, Žikić RV, Stajn AS, Saičić Z. Effect of chronic cadmium exposure on antioxidant defense system in some tissues of rats: Protective effect of selenium. in Physiological Research. 2008;57(3):null-411.
https://hdl.handle.net/21.15107/rcub_ibiss_1562 .

Ognjanović, Branka I, Marković, Snezana D, Pavlović, Slađan, Žikić, Radoslav V., Stajn, Andras S, Saičić, Zorica, "Effect of chronic cadmium exposure on antioxidant defense system in some tissues of rats: Protective effect of selenium" in Physiological Research, 57, no. 3 (2008),
https://hdl.handle.net/21.15107/rcub_ibiss_1562 .

TY  - JOUR
AU  - Marković, Snezana D
AU  - Vukajlović, Miroslava Đ
AU  - Ognjanović, Branka I
AU  - Stajn, Andras S
AU  - Žikić, Radoslav V.
AU  - Saičić, Zorica
AU  - Radojičić, Ratko M.
AU  - Jones, David R
AU  - Spasić, Mihajlo
PY  - 2007
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1602
AB  - After enzymic biotransformation, molsidomine (MO) acts via the metabolite 3-morpholmosydnonimine (SIN-1) through spontaneous liberation of nitric oxide (NO) and superoxide (O-2(.-)). The aim of this study was to compare the effects of MO and its active metabolite SIN-1 on the redox status of rat erythrocytes and reticulocytes. Rat erythrocyte as well as reticulocyte-rich red blood cell (RBC) suspensions were aerobically incubated (2h, 37 degrees C) without (control) or in the presence of different concentrations of MO or SIN-1. In rat erythrocytes, biotransformation of MO resulted in the production of NO and nitroxyl (NO-). Endogenous superoxide anion (O-2(.-)) participated in peroxynitrite generation. SIN-1 simultaneously liberated NO and O-2(.-), which formed peroxynitrite (at least in part), but the liberated NO predominantly reacted with haemoglobin, forming methaemoglobin in erythrocytes. In reticulocytes, MO and SIN-1 caused an increase in the levels of both nitrite and 3-nitrotyrosine (an indicator of peroxynitrite), whereas they decreased the level of O-2(.-). In reticulocytes, MO was metabolized into SIN-1 which led to the generation of NO, which reacted with O-2(.-) (endogenous or exogenous) forming reactive nitrogen species. In conclusion, there are two metabolic pathways for MO biotransformation: one causing NO and NO- generation predominantly in erythrocytes and the other, via SINA metabolism, in reticulocytes. The main difference between the action of MO and SIN-1 was that the latter caused oxidative damage in RBCs. Copyright (c) 2006 John Wiley & Sons, Ltd.
T2  - Cell Biochemistry and Function
T1  - A comparative study of the effects of molsidomine and 3-morpholinosydnonimine on the redox status of rat erythrocytes and reticulocytes
IS  - 3
VL  - 25
EP  - 258
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1602
ER  - 

@article{
author = "Marković, Snezana D and Vukajlović, Miroslava Đ and Ognjanović, Branka I and Stajn, Andras S and Žikić, Radoslav V. and Saičić, Zorica and Radojičić, Ratko M. and Jones, David R and Spasić, Mihajlo",
year = "2007",
abstract = "After enzymic biotransformation, molsidomine (MO) acts via the metabolite 3-morpholmosydnonimine (SIN-1) through spontaneous liberation of nitric oxide (NO) and superoxide (O-2(.-)). The aim of this study was to compare the effects of MO and its active metabolite SIN-1 on the redox status of rat erythrocytes and reticulocytes. Rat erythrocyte as well as reticulocyte-rich red blood cell (RBC) suspensions were aerobically incubated (2h, 37 degrees C) without (control) or in the presence of different concentrations of MO or SIN-1. In rat erythrocytes, biotransformation of MO resulted in the production of NO and nitroxyl (NO-). Endogenous superoxide anion (O-2(.-)) participated in peroxynitrite generation. SIN-1 simultaneously liberated NO and O-2(.-), which formed peroxynitrite (at least in part), but the liberated NO predominantly reacted with haemoglobin, forming methaemoglobin in erythrocytes. In reticulocytes, MO and SIN-1 caused an increase in the levels of both nitrite and 3-nitrotyrosine (an indicator of peroxynitrite), whereas they decreased the level of O-2(.-). In reticulocytes, MO was metabolized into SIN-1 which led to the generation of NO, which reacted with O-2(.-) (endogenous or exogenous) forming reactive nitrogen species. In conclusion, there are two metabolic pathways for MO biotransformation: one causing NO and NO- generation predominantly in erythrocytes and the other, via SINA metabolism, in reticulocytes. The main difference between the action of MO and SIN-1 was that the latter caused oxidative damage in RBCs. Copyright (c) 2006 John Wiley & Sons, Ltd.",
journal = "Cell Biochemistry and Function",
title = "A comparative study of the effects of molsidomine and 3-morpholinosydnonimine on the redox status of rat erythrocytes and reticulocytes",
number = "3",
volume = "25",
pages = "258",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1602"
}

Marković, S. D., Vukajlović, M. Đ., Ognjanović, B. I., Stajn, A. S., Žikić, R. V., Saičić, Z., Radojičić, R. M., Jones, D. R.,& Spasić, M.. (2007). A comparative study of the effects of molsidomine and 3-morpholinosydnonimine on the redox status of rat erythrocytes and reticulocytes. in Cell Biochemistry and Function, 25(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1602

Marković SD, Vukajlović MĐ, Ognjanović BI, Stajn AS, Žikić RV, Saičić Z, Radojičić RM, Jones DR, Spasić M. A comparative study of the effects of molsidomine and 3-morpholinosydnonimine on the redox status of rat erythrocytes and reticulocytes. in Cell Biochemistry and Function. 2007;25(3):null-258.
https://hdl.handle.net/21.15107/rcub_ibiss_1602 .

Marković, Snezana D, Vukajlović, Miroslava Đ, Ognjanović, Branka I, Stajn, Andras S, Žikić, Radoslav V., Saičić, Zorica, Radojičić, Ratko M., Jones, David R, Spasić, Mihajlo, "A comparative study of the effects of molsidomine and 3-morpholinosydnonimine on the redox status of rat erythrocytes and reticulocytes" in Cell Biochemistry and Function, 25, no. 3 (2007),
https://hdl.handle.net/21.15107/rcub_ibiss_1602 .

TY  - JOUR
AU  - Žikić, Radoslav V.
AU  - Ognjanović, Branka I
AU  - Marković, Snezana D
AU  - Pavlović, Slađan
AU  - Mihajlović, Ranđel P
AU  - Saičić, Zorica
AU  - Stajn, Andras S
PY  - 2006
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1650
AB  - Background and Purpose: Recent investigations in biomonitoring include the follow-up of biochemical and physiological parameters in order to estimate the condition of the environment. Oxidative stress parameters are significant biomarkers of ecological pollution of the sea. Materials and Methods: Specimens of red mullet (Mullus barbatus) were collected from the seawater at the localities of Platamuni and Valdanos (in the Adriatic Sea) both in winter and spring. The concentrations of lipid peroxides (LP), vitamin E (vit E) and vitamin C (vit C) were determined both in the liver and white muscle. Salinity, temperature, oxygen saturation, concentrations of nitrates and nitrites and detergents were determined in the seawater at the time of specimen collection. Results and Conclusions: The obtained results showed higher concentration of LP, vit E and vit C in the liver of red mullet when compared to white muscle. The concentrations of LP and vit C were higher, whereas the vit E concentration was lower in winter than in spring. In both the liver and white muscle (from Valdanos), higher concentration of LP and lower concentration of vit E were found when compared to those from Platamuni. Such changes in the oxidative stress parameters were in correlation with seasonal changes in temperature and the concentrations of nitrates, nitrites and detergents in the water. The intensity of oxidative stress in liver of red mullet was higher in comparison to muscles. It was also higher in winter compared to spring, and higher in Valdanos than in Platamuni. In conclusion, parameters of oxidative stress in tissues of red mullet are good markers for the biomonitoring of environment conditions.
T2  - Periodicum Biologorum
T1  - Lipid peroxidation and the concentration of antioxidant compounds (vitamin E and vitamin C) in the liver and white muscle of red mullet (Mullus barbatus L.) from the Adriatic Sea
IS  - 2
VL  - 108
EP  - 143
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1650
ER  - 

@article{
author = "Žikić, Radoslav V. and Ognjanović, Branka I and Marković, Snezana D and Pavlović, Slađan and Mihajlović, Ranđel P and Saičić, Zorica and Stajn, Andras S",
year = "2006",
abstract = "Background and Purpose: Recent investigations in biomonitoring include the follow-up of biochemical and physiological parameters in order to estimate the condition of the environment. Oxidative stress parameters are significant biomarkers of ecological pollution of the sea. Materials and Methods: Specimens of red mullet (Mullus barbatus) were collected from the seawater at the localities of Platamuni and Valdanos (in the Adriatic Sea) both in winter and spring. The concentrations of lipid peroxides (LP), vitamin E (vit E) and vitamin C (vit C) were determined both in the liver and white muscle. Salinity, temperature, oxygen saturation, concentrations of nitrates and nitrites and detergents were determined in the seawater at the time of specimen collection. Results and Conclusions: The obtained results showed higher concentration of LP, vit E and vit C in the liver of red mullet when compared to white muscle. The concentrations of LP and vit C were higher, whereas the vit E concentration was lower in winter than in spring. In both the liver and white muscle (from Valdanos), higher concentration of LP and lower concentration of vit E were found when compared to those from Platamuni. Such changes in the oxidative stress parameters were in correlation with seasonal changes in temperature and the concentrations of nitrates, nitrites and detergents in the water. The intensity of oxidative stress in liver of red mullet was higher in comparison to muscles. It was also higher in winter compared to spring, and higher in Valdanos than in Platamuni. In conclusion, parameters of oxidative stress in tissues of red mullet are good markers for the biomonitoring of environment conditions.",
journal = "Periodicum Biologorum",
title = "Lipid peroxidation and the concentration of antioxidant compounds (vitamin E and vitamin C) in the liver and white muscle of red mullet (Mullus barbatus L.) from the Adriatic Sea",
number = "2",
volume = "108",
pages = "143",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1650"
}

Žikić, R. V., Ognjanović, B. I., Marković, S. D., Pavlović, S., Mihajlović, R. P., Saičić, Z.,& Stajn, A. S.. (2006). Lipid peroxidation and the concentration of antioxidant compounds (vitamin E and vitamin C) in the liver and white muscle of red mullet (Mullus barbatus L.) from the Adriatic Sea. in Periodicum Biologorum, 108(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1650

Žikić RV, Ognjanović BI, Marković SD, Pavlović S, Mihajlović RP, Saičić Z, Stajn AS. Lipid peroxidation and the concentration of antioxidant compounds (vitamin E and vitamin C) in the liver and white muscle of red mullet (Mullus barbatus L.) from the Adriatic Sea. in Periodicum Biologorum. 2006;108(2):null-143.
https://hdl.handle.net/21.15107/rcub_ibiss_1650 .

Žikić, Radoslav V., Ognjanović, Branka I, Marković, Snezana D, Pavlović, Slađan, Mihajlović, Ranđel P, Saičić, Zorica, Stajn, Andras S, "Lipid peroxidation and the concentration of antioxidant compounds (vitamin E and vitamin C) in the liver and white muscle of red mullet (Mullus barbatus L.) from the Adriatic Sea" in Periodicum Biologorum, 108, no. 2 (2006),
https://hdl.handle.net/21.15107/rcub_ibiss_1650 .

TY  - JOUR
AU  - Ognjanović, Branka I
AU  - Marković, Snezana D
AU  - Pavlović, Slađan
AU  - Žikić, Radoslav V.
AU  - Stajn, Andras S
AU  - Saičić, Zorica
PY  - 2006
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1634
AB  - Our study investigated the possible protective effects of coenzyme Q(10) (CoQ(10)) and Vitamin E (Vit E) alone or in combination against cadmium (Cd) induced alterations of antioxidant defense system in the rat heart. Male Wistar rats were injected with a single dose of CdCl(2) (0.4 mg Cd/kg BW i.p.), CoQ(10) (20 mg CoQ(10)/kg BW i.m.) and Vit E (20 IU Vit E/kg BW i.m.), alone or in combination. Acute intoxication of rats with Cd were followed by significantly increased activity of antioxidant defense enzymes (CuZn SOD, GSH-Px, GST and GR), while the activity of Mn SOD was decreased in the heart. The treatment with Cd significantly decreased Vit C and Vit E concentrations. Treatment with CoQ10 and Vit E reversed Cd-induced alterations of antioxidant defense system. The obtained results support the assumption that CoQ10 and Vit E functions cooperatively with endogenous antioxidants and diminished toxic effects of Cd in rat heart. (c) 2006 Elsevier B.V. All rights reserved.
T2  - Environmental Toxicology and Pharmacology
T1  - Combined effects of coenzyme Q(10) and Vitamin E in cadmium induced alterations of antioxidant defense system in the rat heart
IS  - 2
VL  - 22
EP  - 224
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1634
ER  - 

@article{
author = "Ognjanović, Branka I and Marković, Snezana D and Pavlović, Slađan and Žikić, Radoslav V. and Stajn, Andras S and Saičić, Zorica",
year = "2006",
abstract = "Our study investigated the possible protective effects of coenzyme Q(10) (CoQ(10)) and Vitamin E (Vit E) alone or in combination against cadmium (Cd) induced alterations of antioxidant defense system in the rat heart. Male Wistar rats were injected with a single dose of CdCl(2) (0.4 mg Cd/kg BW i.p.), CoQ(10) (20 mg CoQ(10)/kg BW i.m.) and Vit E (20 IU Vit E/kg BW i.m.), alone or in combination. Acute intoxication of rats with Cd were followed by significantly increased activity of antioxidant defense enzymes (CuZn SOD, GSH-Px, GST and GR), while the activity of Mn SOD was decreased in the heart. The treatment with Cd significantly decreased Vit C and Vit E concentrations. Treatment with CoQ10 and Vit E reversed Cd-induced alterations of antioxidant defense system. The obtained results support the assumption that CoQ10 and Vit E functions cooperatively with endogenous antioxidants and diminished toxic effects of Cd in rat heart. (c) 2006 Elsevier B.V. All rights reserved.",
journal = "Environmental Toxicology and Pharmacology",
title = "Combined effects of coenzyme Q(10) and Vitamin E in cadmium induced alterations of antioxidant defense system in the rat heart",
number = "2",
volume = "22",
pages = "224",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1634"
}

Ognjanović, B. I., Marković, S. D., Pavlović, S., Žikić, R. V., Stajn, A. S.,& Saičić, Z.. (2006). Combined effects of coenzyme Q(10) and Vitamin E in cadmium induced alterations of antioxidant defense system in the rat heart. in Environmental Toxicology and Pharmacology, 22(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1634

Ognjanović BI, Marković SD, Pavlović S, Žikić RV, Stajn AS, Saičić Z. Combined effects of coenzyme Q(10) and Vitamin E in cadmium induced alterations of antioxidant defense system in the rat heart. in Environmental Toxicology and Pharmacology. 2006;22(2):null-224.
https://hdl.handle.net/21.15107/rcub_ibiss_1634 .

Ognjanović, Branka I, Marković, Snezana D, Pavlović, Slađan, Žikić, Radoslav V., Stajn, Andras S, Saičić, Zorica, "Combined effects of coenzyme Q(10) and Vitamin E in cadmium induced alterations of antioxidant defense system in the rat heart" in Environmental Toxicology and Pharmacology, 22, no. 2 (2006),
https://hdl.handle.net/21.15107/rcub_ibiss_1634 .

TY  - JOUR
AU  - Marković, Snezana D
AU  - Ognjanović, Branka I.
AU  - Stajn, Andras S
AU  - Žikić, Radoslav
AU  - Saičić, Zorica
AU  - Radojičić, Ratko M.
AU  - Spasić, Mihajlo
PY  - 2006
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1670
AB  - The effects of nitroglycerine (NTG) are mediated by liberated nitric oxide (NO) after NTG enzymatic biotransformation in cells. The aim of this study was to evaluate some products of NTG bio-transformation and their consequences on the redox status of rat erythrocytes and reticulocytes, considering the absence and presence of functional mitochondria in these cells, respectively. Rat erythrocyte and reticulocyte-rich red blood cell (RBC) suspensions were aerobically incubated (2h, 37 degrees C) without (control) or in the presence of different concentrations of NTG (0.1, 0.25, 0.5, 1.0 and 1.5 mM). In rat erythrocytes, NTG did not elevate the concentrations of any reactive nitrogen species (RNS). However, NTG robustly increased concentration of methemoglobin (MetHb), suggesting that NTG bio-transformation was primarily connected with hemoglobin (Hb). NTG-induced MetHb formation was followed by the induction of lipid peroxidation. In rat reticulocytes, NTG caused an increase in the levels of nitrite, peroxinitrite, hydrogen peroxide, MetHb and lipid peroxide levels, but it decreased the level of the superoxide anion radical. Millimolar concentrations of NTG caused oxidative damage of both erythrocytes and reticulocytes. These data indicate that two pathways of NTG bio-transformation exist in reticulocytes: one generating RNS and the other connected with Hb (as in erythrocytes). In conclusion, NTG bio-transformation is different in erythrocytes and reticulocytes due to the presence of mitochondria in the latter.
T2  - Physiological Research
T1  - The effects of nitroglycerine on the redox status of rat erythrocytes and reticulocytes
IS  - 4
VL  - 55
EP  - 396
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1670
ER  - 

@article{
author = "Marković, Snezana D and Ognjanović, Branka I. and Stajn, Andras S and Žikić, Radoslav and Saičić, Zorica and Radojičić, Ratko M. and Spasić, Mihajlo",
year = "2006",
abstract = "The effects of nitroglycerine (NTG) are mediated by liberated nitric oxide (NO) after NTG enzymatic biotransformation in cells. The aim of this study was to evaluate some products of NTG bio-transformation and their consequences on the redox status of rat erythrocytes and reticulocytes, considering the absence and presence of functional mitochondria in these cells, respectively. Rat erythrocyte and reticulocyte-rich red blood cell (RBC) suspensions were aerobically incubated (2h, 37 degrees C) without (control) or in the presence of different concentrations of NTG (0.1, 0.25, 0.5, 1.0 and 1.5 mM). In rat erythrocytes, NTG did not elevate the concentrations of any reactive nitrogen species (RNS). However, NTG robustly increased concentration of methemoglobin (MetHb), suggesting that NTG bio-transformation was primarily connected with hemoglobin (Hb). NTG-induced MetHb formation was followed by the induction of lipid peroxidation. In rat reticulocytes, NTG caused an increase in the levels of nitrite, peroxinitrite, hydrogen peroxide, MetHb and lipid peroxide levels, but it decreased the level of the superoxide anion radical. Millimolar concentrations of NTG caused oxidative damage of both erythrocytes and reticulocytes. These data indicate that two pathways of NTG bio-transformation exist in reticulocytes: one generating RNS and the other connected with Hb (as in erythrocytes). In conclusion, NTG bio-transformation is different in erythrocytes and reticulocytes due to the presence of mitochondria in the latter.",
journal = "Physiological Research",
title = "The effects of nitroglycerine on the redox status of rat erythrocytes and reticulocytes",
number = "4",
volume = "55",
pages = "396",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1670"
}

Marković, S. D., Ognjanović, B. I., Stajn, A. S., Žikić, R., Saičić, Z., Radojičić, R. M.,& Spasić, M.. (2006). The effects of nitroglycerine on the redox status of rat erythrocytes and reticulocytes. in Physiological Research, 55(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1670

Marković SD, Ognjanović BI, Stajn AS, Žikić R, Saičić Z, Radojičić RM, Spasić M. The effects of nitroglycerine on the redox status of rat erythrocytes and reticulocytes. in Physiological Research. 2006;55(4):null-396.
https://hdl.handle.net/21.15107/rcub_ibiss_1670 .

Marković, Snezana D, Ognjanović, Branka I., Stajn, Andras S, Žikić, Radoslav, Saičić, Zorica, Radojičić, Ratko M., Spasić, Mihajlo, "The effects of nitroglycerine on the redox status of rat erythrocytes and reticulocytes" in Physiological Research, 55, no. 4 (2006),
https://hdl.handle.net/21.15107/rcub_ibiss_1670 .