TY  - JOUR
AU  - Chiavaroli, Annalisa
AU  - Sinan, Koaudio Ibrahime
AU  - Zengin, Gokhan
AU  - Mahomoodally, Mohamad Fawzi
AU  - Bibi Sadeer, Nabeelah
AU  - Etienne, Ouattara Katinan
AU  - Cziáky, Zoltán
AU  - Jekő, József
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Recinella, Lucia
AU  - Brunetti, Luigi
AU  - Leone, Sheila
AU  - Abdallah, Hassan H.
AU  - Angelini, Paola
AU  - Angeles Flores, Giancarlo
AU  - Venanzoni, Roberto
AU  - Menghini, Luigi
AU  - Orlando, Giustino
AU  - Ferrante, Claudio
PY  - 2020
UR  - https://www.mdpi.com/2076-3921/9/6/533
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3719
AB  - Mangrove forests exemplify a multifaceted ecosystem since they do not only play a crucial ecological role but also possess medicinal properties. Methanolic, ethyl acetate and aqueous leaf and bark extracts were prepared using homogenizer-assisted extraction (HAE), infusion and maceration (with and without stirring). The different extracts were screened for phytochemical profiling and antioxidant capacities in terms of radical scavenging (DPPH, ABTS), reducing potential (CUPRAC, FRAP), total antioxidant capacity and chelating power. Additionally, R. racemosa was evaluated for its anti-diabetic (α-amylase, α-glucosidase), anti-tyrosinase and anti-cholinesterase (AChE, BChE) activities. Additionally, antimycotic and antibacterial effects were investigated against Eescherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium, Listeria monocytogenes, Enterobacter cloacae, Bacillus cereus, Micrococcus luteus, Staphylococcus aureus, Aspergillus fumigatus, Aspergillus niger, Trichoderma viride, Penicillium funiculosum, Penicillium ochrochloron and Penicillium verrucosum. Finally, based on phytochemical fingerprint, in silico studies, including bioinformatics, network pharmacology and docking approaches were conducted to predict the putative targets, namely tyrosinase, lanosterol-14-α-demethylase and E. coli DNA gyrase, underlying the observed bio-pharmacological and microbiological effects. The methanolic leave and bark extracts (prepared by both HAE and maceration) abounded with phenolics, flavonoids, phenolic acids and flavonols. Results displayed that both methanolic leaf and bark extracts (prepared by HAE) exhibited the highest radical scavenging, reducing potential and total antioxidant capacity. Furthermore, our findings showed that the highest enzymatic inhibitory activity recorded was with the tyrosinase enzyme. In this context, bioinformatics analysis predicted putative interactions between tyrosinase and multiple secondary metabolites including apigenin, luteolin, vitexin, isovitexin, procyanidin B, quercetin and methoxy-trihydroxyflavone. The same compounds were also docked against lanosterol-14α-demethylase and E. Coli DNA gyrase, yielding affinities in the submicromolar–micromolar range that further support the observed anti-microbial effects exerted by the extracts. In conclusion, extracts of R. racemosa may be considered as novel sources of phytoanti-oxidants and enzyme inhibitors that can be exploited as future first-line pharmacophores.
PB  - MDPI AG
T2  - Antioxidants
T1  - Identification of Chemical Profiles and Biological Properties of Rhizophora racemosa G. Mey. Extracts Obtained by Different Methods and Solvents
IS  - 6
VL  - 9
DO  - 10.3390/antiox9060533
SP  - 533
ER  - 

@article{
author = "Chiavaroli, Annalisa and Sinan, Koaudio Ibrahime and Zengin, Gokhan and Mahomoodally, Mohamad Fawzi and Bibi Sadeer, Nabeelah and Etienne, Ouattara Katinan and Cziáky, Zoltán and Jekő, József and Glamočlija, Jasmina and Soković, Marina and Recinella, Lucia and Brunetti, Luigi and Leone, Sheila and Abdallah, Hassan H. and Angelini, Paola and Angeles Flores, Giancarlo and Venanzoni, Roberto and Menghini, Luigi and Orlando, Giustino and Ferrante, Claudio",
year = "2020",
abstract = "Mangrove forests exemplify a multifaceted ecosystem since they do not only play a crucial ecological role but also possess medicinal properties. Methanolic, ethyl acetate and aqueous leaf and bark extracts were prepared using homogenizer-assisted extraction (HAE), infusion and maceration (with and without stirring). The different extracts were screened for phytochemical profiling and antioxidant capacities in terms of radical scavenging (DPPH, ABTS), reducing potential (CUPRAC, FRAP), total antioxidant capacity and chelating power. Additionally, R. racemosa was evaluated for its anti-diabetic (α-amylase, α-glucosidase), anti-tyrosinase and anti-cholinesterase (AChE, BChE) activities. Additionally, antimycotic and antibacterial effects were investigated against Eescherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium, Listeria monocytogenes, Enterobacter cloacae, Bacillus cereus, Micrococcus luteus, Staphylococcus aureus, Aspergillus fumigatus, Aspergillus niger, Trichoderma viride, Penicillium funiculosum, Penicillium ochrochloron and Penicillium verrucosum. Finally, based on phytochemical fingerprint, in silico studies, including bioinformatics, network pharmacology and docking approaches were conducted to predict the putative targets, namely tyrosinase, lanosterol-14-α-demethylase and E. coli DNA gyrase, underlying the observed bio-pharmacological and microbiological effects. The methanolic leave and bark extracts (prepared by both HAE and maceration) abounded with phenolics, flavonoids, phenolic acids and flavonols. Results displayed that both methanolic leaf and bark extracts (prepared by HAE) exhibited the highest radical scavenging, reducing potential and total antioxidant capacity. Furthermore, our findings showed that the highest enzymatic inhibitory activity recorded was with the tyrosinase enzyme. In this context, bioinformatics analysis predicted putative interactions between tyrosinase and multiple secondary metabolites including apigenin, luteolin, vitexin, isovitexin, procyanidin B, quercetin and methoxy-trihydroxyflavone. The same compounds were also docked against lanosterol-14α-demethylase and E. Coli DNA gyrase, yielding affinities in the submicromolar–micromolar range that further support the observed anti-microbial effects exerted by the extracts. In conclusion, extracts of R. racemosa may be considered as novel sources of phytoanti-oxidants and enzyme inhibitors that can be exploited as future first-line pharmacophores.",
publisher = "MDPI AG",
journal = "Antioxidants",
title = "Identification of Chemical Profiles and Biological Properties of Rhizophora racemosa G. Mey. Extracts Obtained by Different Methods and Solvents",
number = "6",
volume = "9",
doi = "10.3390/antiox9060533",
pages = "533"
}

Chiavaroli, A., Sinan, K. I., Zengin, G., Mahomoodally, M. F., Bibi Sadeer, N., Etienne, O. K., Cziáky, Z., Jekő, J., Glamočlija, J., Soković, M., Recinella, L., Brunetti, L., Leone, S., Abdallah, H. H., Angelini, P., Angeles Flores, G., Venanzoni, R., Menghini, L., Orlando, G.,& Ferrante, C.. (2020). Identification of Chemical Profiles and Biological Properties of Rhizophora racemosa G. Mey. Extracts Obtained by Different Methods and Solvents. in Antioxidants
MDPI AG., 9(6), 533.
https://doi.org/10.3390/antiox9060533

Chiavaroli A, Sinan KI, Zengin G, Mahomoodally MF, Bibi Sadeer N, Etienne OK, Cziáky Z, Jekő J, Glamočlija J, Soković M, Recinella L, Brunetti L, Leone S, Abdallah HH, Angelini P, Angeles Flores G, Venanzoni R, Menghini L, Orlando G, Ferrante C. Identification of Chemical Profiles and Biological Properties of Rhizophora racemosa G. Mey. Extracts Obtained by Different Methods and Solvents. in Antioxidants. 2020;9(6):533.
doi:10.3390/antiox9060533 .

Chiavaroli, Annalisa, Sinan, Koaudio Ibrahime, Zengin, Gokhan, Mahomoodally, Mohamad Fawzi, Bibi Sadeer, Nabeelah, Etienne, Ouattara Katinan, Cziáky, Zoltán, Jekő, József, Glamočlija, Jasmina, Soković, Marina, Recinella, Lucia, Brunetti, Luigi, Leone, Sheila, Abdallah, Hassan H., Angelini, Paola, Angeles Flores, Giancarlo, Venanzoni, Roberto, Menghini, Luigi, Orlando, Giustino, Ferrante, Claudio, "Identification of Chemical Profiles and Biological Properties of Rhizophora racemosa G. Mey. Extracts Obtained by Different Methods and Solvents" in Antioxidants, 9, no. 6 (2020):533,
https://doi.org/10.3390/antiox9060533 . .

TY  - JOUR
AU  - Zengin, Gokhan
AU  - Sinan, Kouadio Ibrahime
AU  - Ak, Gunes
AU  - Mahomoodally, Mohamad Fawzi
AU  - Paksoy, Mehmet Yavuz
AU  - Picot-Allain, Carene
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Jekő, József
AU  - Cziáky, Zoltán
AU  - Rodrigues, Maria João
AU  - Pereira, Catarina G.
AU  - Custodio, Luisa
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3700
AB  - Several Apiaceae species, used as both food and in complementary and alternative medicine, represents a rich source of potential valuable phytopharmaceuticals which necessitates scientific contemplation. In the present study, the antioxidant, enzyme inhibitory, antimicrobial, and cytotoxic properties of methanol extracts of seven Apiaceae species, (Chaerophyllum macrospermum (Willd. ex Spreng.) Fisch. & C.A.Mey. ex Hohen, Ferula rigidula Fisch. ex DC., Ferula orientalis L., Prangos ferulacea Lindl., Prangos peucedanifolia Fenzl., Ferulago setifolia K. Koch, and Pimpinella anthriscoides Boiss.) were evaluated. Species belonging to the Prangos genus exhibited the highest total phenolic content, namely P. peucedanifolia and P. ferulacea, with values of 47.90 and 44.44 mg gallic acid equivalent/g extract, respectively. P. peucedanifolia also displayed the highest radical scavenging capacity (81.53 and 102.70 mg Trolox equivalent [TE]/g extract for 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS), respectively) and reducing power (165.87 and 100.09 mg TE/g extract for cupric reducing antioxidant capacity (CUPRAC) and ferric reducing antioxidant power (FRAP), respectively). C. macrospermum showed the most potent inhibition against Alzheimer's disease related enzymes, namely acetylcholinesterase (4.53 mg galantamine equivalent [GALAE]/g extract) and butyrylcholinesterase (3.22 mg GALAE/g extract). P. ferulacea (131.94 mg kojic acid (KAE) equivalent/g extract) and P. peucedanifolia (4.97 mmol acarbose equivalent (ACAE)/g extract) were potent inhibitors of tyrosinase and α-glucosidase, respectively. In general, studied species were able to reduce cellular viabilities. P. peucedanifolia possessed promising antibacterial potential against Bacillus cereus (Minimum inhibition concentration (MIC): 0.37 mg/mL), L. monocytogenes (MIC: 0.56 mg/mL), P. aeruginosa and Escherichia coli (MIC: 0.27 mg/mL), Salmonella typhimurium and Enterobacter cloacae (MIC: 0.75 mg/mL). F. rigidula showed the highest antifungal effect against Aspergillus ochraceus and Trichoderma viride (MIC: 0.10 mg/mL). The present findings could be the scientific starting point towards the pharmaceutical and/or commercial utilization of these Apiaceae species.
PB  - Elsevier B.V.
T2  - Industrial Crops and Products
T1  - Chemical profile, antioxidant, antimicrobial, enzyme inhibitory, and cytotoxicity of seven Apiaceae species from Turkey: A comparative study
VL  - 153
DO  - 10.1016/j.indcrop.2020.112572
SP  - 112572
ER  - 

@article{
author = "Zengin, Gokhan and Sinan, Kouadio Ibrahime and Ak, Gunes and Mahomoodally, Mohamad Fawzi and Paksoy, Mehmet Yavuz and Picot-Allain, Carene and Glamočlija, Jasmina and Soković, Marina and Jekő, József and Cziáky, Zoltán and Rodrigues, Maria João and Pereira, Catarina G. and Custodio, Luisa",
year = "2020",
abstract = "Several Apiaceae species, used as both food and in complementary and alternative medicine, represents a rich source of potential valuable phytopharmaceuticals which necessitates scientific contemplation. In the present study, the antioxidant, enzyme inhibitory, antimicrobial, and cytotoxic properties of methanol extracts of seven Apiaceae species, (Chaerophyllum macrospermum (Willd. ex Spreng.) Fisch. & C.A.Mey. ex Hohen, Ferula rigidula Fisch. ex DC., Ferula orientalis L., Prangos ferulacea Lindl., Prangos peucedanifolia Fenzl., Ferulago setifolia K. Koch, and Pimpinella anthriscoides Boiss.) were evaluated. Species belonging to the Prangos genus exhibited the highest total phenolic content, namely P. peucedanifolia and P. ferulacea, with values of 47.90 and 44.44 mg gallic acid equivalent/g extract, respectively. P. peucedanifolia also displayed the highest radical scavenging capacity (81.53 and 102.70 mg Trolox equivalent [TE]/g extract for 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS), respectively) and reducing power (165.87 and 100.09 mg TE/g extract for cupric reducing antioxidant capacity (CUPRAC) and ferric reducing antioxidant power (FRAP), respectively). C. macrospermum showed the most potent inhibition against Alzheimer's disease related enzymes, namely acetylcholinesterase (4.53 mg galantamine equivalent [GALAE]/g extract) and butyrylcholinesterase (3.22 mg GALAE/g extract). P. ferulacea (131.94 mg kojic acid (KAE) equivalent/g extract) and P. peucedanifolia (4.97 mmol acarbose equivalent (ACAE)/g extract) were potent inhibitors of tyrosinase and α-glucosidase, respectively. In general, studied species were able to reduce cellular viabilities. P. peucedanifolia possessed promising antibacterial potential against Bacillus cereus (Minimum inhibition concentration (MIC): 0.37 mg/mL), L. monocytogenes (MIC: 0.56 mg/mL), P. aeruginosa and Escherichia coli (MIC: 0.27 mg/mL), Salmonella typhimurium and Enterobacter cloacae (MIC: 0.75 mg/mL). F. rigidula showed the highest antifungal effect against Aspergillus ochraceus and Trichoderma viride (MIC: 0.10 mg/mL). The present findings could be the scientific starting point towards the pharmaceutical and/or commercial utilization of these Apiaceae species.",
publisher = "Elsevier B.V.",
journal = "Industrial Crops and Products",
title = "Chemical profile, antioxidant, antimicrobial, enzyme inhibitory, and cytotoxicity of seven Apiaceae species from Turkey: A comparative study",
volume = "153",
doi = "10.1016/j.indcrop.2020.112572",
pages = "112572"
}

Zengin, G., Sinan, K. I., Ak, G., Mahomoodally, M. F., Paksoy, M. Y., Picot-Allain, C., Glamočlija, J., Soković, M., Jekő, J., Cziáky, Z., Rodrigues, M. J., Pereira, C. G.,& Custodio, L.. (2020). Chemical profile, antioxidant, antimicrobial, enzyme inhibitory, and cytotoxicity of seven Apiaceae species from Turkey: A comparative study. in Industrial Crops and Products
Elsevier B.V.., 153, 112572.
https://doi.org/10.1016/j.indcrop.2020.112572

Zengin G, Sinan KI, Ak G, Mahomoodally MF, Paksoy MY, Picot-Allain C, Glamočlija J, Soković M, Jekő J, Cziáky Z, Rodrigues MJ, Pereira CG, Custodio L. Chemical profile, antioxidant, antimicrobial, enzyme inhibitory, and cytotoxicity of seven Apiaceae species from Turkey: A comparative study. in Industrial Crops and Products. 2020;153:112572.
doi:10.1016/j.indcrop.2020.112572 .

Zengin, Gokhan, Sinan, Kouadio Ibrahime, Ak, Gunes, Mahomoodally, Mohamad Fawzi, Paksoy, Mehmet Yavuz, Picot-Allain, Carene, Glamočlija, Jasmina, Soković, Marina, Jekő, József, Cziáky, Zoltán, Rodrigues, Maria João, Pereira, Catarina G., Custodio, Luisa, "Chemical profile, antioxidant, antimicrobial, enzyme inhibitory, and cytotoxicity of seven Apiaceae species from Turkey: A comparative study" in Industrial Crops and Products, 153 (2020):112572,
https://doi.org/10.1016/j.indcrop.2020.112572 . .

TY  - JOUR
AU  - Zengin, Gokhan
AU  - Paksoy, Mehmet Yavuz
AU  - Aumeeruddy, Muhammad Zakariyyah
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Diuzheva, Alina
AU  - Jekő, József
AU  - Cziáky, Zoltán
AU  - Rodrigues, Maria João
AU  - Custodio, Luisa
AU  - Mahomoodally, Mohamad Fawzi
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0963996919303163?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3407
AB  - Bunium species have been reported to be used both as food and in traditional medicines. The scientific community has attempted to probe into the pharmacological and chemical profiles of this genus. Nonetheless, many species have not been investigated fully to date. In this study, we determined the phenolic components, antimicrobial, antioxidant, and enzyme inhibitory activities of aerial parts of four Bunium species (B. sayai, B. pinnatifolium, B. brachyactis and B. macrocarpum). Results showed that B. microcarpum and B. pinnatifolium were strong antioxidants as evidenced in the DPPH, ABTS, CUPRAC, and FRAP assays. B. brachyactis was the most effective metal chelator, and displayed high enzyme inhibition against cholinesterase, tyrosinase, amylase, glucosidase, and lipase. The four species showed varied antimicrobial activity against each microorganism. Overall, they showed high activity against P. mirabilis and E. coli (MIC and MBC <1 mg mL−1). B. brachyactis was more effective against Aspergillus versicolor compared to the standard drug ketoconazole. B. brachyactis was also more effective than both ketoconazole and bifonazole against Trichoderma viride. B. sayai was more effective than ketoconazole in inhibiting A. fumigatus. B. sayai was most non-toxic to HEK 293 (cellular viability = 117%) and HepG2 (cellular viability = 104%). The highest level of TPC was observed in B. pinnatifolium (35.94 mg GAE g−1) while B. microcarpum possessed the highest TFC (39.21 mg RE g−1). Seventy four compounds were detected in B. microcarpum, 70 in B. brachyactis, 66 in B. sayai, and 51 in B. pinnatifolium. Quinic acid, chlorogenic acid, pantothenic acid, esculin, isoquercitrin, rutin, apigenin, and scopoletin were present in all the four species. This study showed that the four Bunium species are good sources of biologically active compounds with pharmaceutical and nutraceutical potential.
T2  - Food Research International
T1  - New insights into the chemical profiling, cytotoxicity and bioactivity of four Bunium species
VL  - 123
DO  - 10.1016/J.FOODRES.2019.05.013
SP  - 414
EP  - 424
ER  - 

@article{
author = "Zengin, Gokhan and Paksoy, Mehmet Yavuz and Aumeeruddy, Muhammad Zakariyyah and Glamočlija, Jasmina and Soković, Marina and Diuzheva, Alina and Jekő, József and Cziáky, Zoltán and Rodrigues, Maria João and Custodio, Luisa and Mahomoodally, Mohamad Fawzi",
year = "2019",
abstract = "Bunium species have been reported to be used both as food and in traditional medicines. The scientific community has attempted to probe into the pharmacological and chemical profiles of this genus. Nonetheless, many species have not been investigated fully to date. In this study, we determined the phenolic components, antimicrobial, antioxidant, and enzyme inhibitory activities of aerial parts of four Bunium species (B. sayai, B. pinnatifolium, B. brachyactis and B. macrocarpum). Results showed that B. microcarpum and B. pinnatifolium were strong antioxidants as evidenced in the DPPH, ABTS, CUPRAC, and FRAP assays. B. brachyactis was the most effective metal chelator, and displayed high enzyme inhibition against cholinesterase, tyrosinase, amylase, glucosidase, and lipase. The four species showed varied antimicrobial activity against each microorganism. Overall, they showed high activity against P. mirabilis and E. coli (MIC and MBC <1 mg mL−1). B. brachyactis was more effective against Aspergillus versicolor compared to the standard drug ketoconazole. B. brachyactis was also more effective than both ketoconazole and bifonazole against Trichoderma viride. B. sayai was more effective than ketoconazole in inhibiting A. fumigatus. B. sayai was most non-toxic to HEK 293 (cellular viability = 117%) and HepG2 (cellular viability = 104%). The highest level of TPC was observed in B. pinnatifolium (35.94 mg GAE g−1) while B. microcarpum possessed the highest TFC (39.21 mg RE g−1). Seventy four compounds were detected in B. microcarpum, 70 in B. brachyactis, 66 in B. sayai, and 51 in B. pinnatifolium. Quinic acid, chlorogenic acid, pantothenic acid, esculin, isoquercitrin, rutin, apigenin, and scopoletin were present in all the four species. This study showed that the four Bunium species are good sources of biologically active compounds with pharmaceutical and nutraceutical potential.",
journal = "Food Research International",
title = "New insights into the chemical profiling, cytotoxicity and bioactivity of four Bunium species",
volume = "123",
doi = "10.1016/J.FOODRES.2019.05.013",
pages = "414-424"
}

Zengin, G., Paksoy, M. Y., Aumeeruddy, M. Z., Glamočlija, J., Soković, M., Diuzheva, A., Jekő, J., Cziáky, Z., Rodrigues, M. J., Custodio, L.,& Mahomoodally, M. F.. (2019). New insights into the chemical profiling, cytotoxicity and bioactivity of four Bunium species. in Food Research International, 123, 414-424.
https://doi.org/10.1016/J.FOODRES.2019.05.013

Zengin G, Paksoy MY, Aumeeruddy MZ, Glamočlija J, Soković M, Diuzheva A, Jekő J, Cziáky Z, Rodrigues MJ, Custodio L, Mahomoodally MF. New insights into the chemical profiling, cytotoxicity and bioactivity of four Bunium species. in Food Research International. 2019;123:414-424.
doi:10.1016/J.FOODRES.2019.05.013 .

Zengin, Gokhan, Paksoy, Mehmet Yavuz, Aumeeruddy, Muhammad Zakariyyah, Glamočlija, Jasmina, Soković, Marina, Diuzheva, Alina, Jekő, József, Cziáky, Zoltán, Rodrigues, Maria João, Custodio, Luisa, Mahomoodally, Mohamad Fawzi, "New insights into the chemical profiling, cytotoxicity and bioactivity of four Bunium species" in Food Research International, 123 (2019):414-424,
https://doi.org/10.1016/J.FOODRES.2019.05.013 . .

TY  - JOUR
AU  - Zengin, Gokhan
AU  - Mahomoodally, Mohamad Fawzi
AU  - Paksoy, Mehmet Yavuz
AU  - Picot-Allain, Carene
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Diuzheva, Alina
AU  - Jekő, József
AU  - Cziáky, Zoltán
AU  - Rodrigues, Maria João
AU  - Sinan, Kouadio Ibrahime
AU  - Custodio, Luisa
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0926669019302833?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3337
AB  - Several species of the Apiaceae family have been employed in traditional cultures for their curative virtues. The present study focused on five Apiaceae species, (Falcaria vulgaris (FV), Smyrniopsis aucheri (SA), Smyrniopsis munzurdagensis (SM), Smyrnium cordifolium (SC), and Actinolema macrolema (AM)). The antioxidant, enzyme inhibitory (α-amylase, α-glucosidase, acetyl- and butyrylcholinesterase, lipase, and tyrosinase), antimicrobial, phytochemical, and cytotoxicity profiles of the methanol extracts of the selected Apiaceae species were determined. SC extract (35.68 mg gallic acid equivalent/g extract) possessed the highest phenolic content while the AM extract (56.79 mg rutin equivalent/g extract) had the highest flavonoid content. HPLC-ESI-MS (High performance liquid chromatography-electrospray tandem mass spectrometry) analyses showed presence of ferulic acid in all the five species. SC extract exhibited high radical scavenging (59.28 and 94.31 mg Trolox equivalent [TE]/g extract, DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS (2,2′-azino-bis(3-ethylbenzothiazoline)-6-sulfonic acid), respectively) and reducing activity (161.44 and 113.62 mg TE/g extract, for CUPRAC (cupric reducing antioxidant capacity) and FRAP (ferric reducing antioxidant power), respectively). SM extract exhibited the highest cholinesterase’s inhibitory action (3.82 and 4.76 mg galantamine equivalent/g extract, for acetyl- and butyrylcholinesterase, respectively). The extracts showed higher inhibition against α-glucosidase (7.32-11.99 mmol acarbose equivalent [ACAE]/g extract) compared to α-amylase (0.51-0.55 mmol ACAE/g extract). SC extract was the most active (137.54 mg kojic acid equivalent/g extract) tyrosinase inhibitor and FV extract (113.75 mg Orlistat equivalent/g) the best lipase inhibitor. SM extract showed potent antibacterial effect against B. cereus (MIC (minimum inhibitory concentration) 0.180 mg/mL), P. mirabilis (MIC 0.180 mg/mL), M. flavus (MIC 0.560 mg/mL), P. aeruginosa (MIC 0.275 mg/mL), and S. typhimurium (MIC 1.500 mg/mL). FV extract (MIC 0.140 mg/mL) suppressed A. fumigatus growth. Cytotoxicity was assessed on murine macrophage (RAW 264.7), human embryonic kidney (HEK 293), and human hepatocellular carcinoma (HepG2) cell lines. FV (60.3%) and SM (57.4%) showed the highest reduction on RAW 264.7 cellular viability, whereas SM (74.1%) showed toxicity against HepG2. This study supports that the Apiaceae species could be considered as promising candidates for the development of novel pharmacophores for the management of several human ailments.
T2  - Industrial Crops and Products
T1  - Phytochemical characterization and bioactivities of five Apiaceae species: Natural sources for novel ingredients
VL  - 135
DO  - 10.1016/J.INDCROP.2019.04.033
SP  - 107
EP  - 121
ER  - 

@article{
author = "Zengin, Gokhan and Mahomoodally, Mohamad Fawzi and Paksoy, Mehmet Yavuz and Picot-Allain, Carene and Glamočlija, Jasmina and Soković, Marina and Diuzheva, Alina and Jekő, József and Cziáky, Zoltán and Rodrigues, Maria João and Sinan, Kouadio Ibrahime and Custodio, Luisa",
year = "2019",
abstract = "Several species of the Apiaceae family have been employed in traditional cultures for their curative virtues. The present study focused on five Apiaceae species, (Falcaria vulgaris (FV), Smyrniopsis aucheri (SA), Smyrniopsis munzurdagensis (SM), Smyrnium cordifolium (SC), and Actinolema macrolema (AM)). The antioxidant, enzyme inhibitory (α-amylase, α-glucosidase, acetyl- and butyrylcholinesterase, lipase, and tyrosinase), antimicrobial, phytochemical, and cytotoxicity profiles of the methanol extracts of the selected Apiaceae species were determined. SC extract (35.68 mg gallic acid equivalent/g extract) possessed the highest phenolic content while the AM extract (56.79 mg rutin equivalent/g extract) had the highest flavonoid content. HPLC-ESI-MS (High performance liquid chromatography-electrospray tandem mass spectrometry) analyses showed presence of ferulic acid in all the five species. SC extract exhibited high radical scavenging (59.28 and 94.31 mg Trolox equivalent [TE]/g extract, DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS (2,2′-azino-bis(3-ethylbenzothiazoline)-6-sulfonic acid), respectively) and reducing activity (161.44 and 113.62 mg TE/g extract, for CUPRAC (cupric reducing antioxidant capacity) and FRAP (ferric reducing antioxidant power), respectively). SM extract exhibited the highest cholinesterase’s inhibitory action (3.82 and 4.76 mg galantamine equivalent/g extract, for acetyl- and butyrylcholinesterase, respectively). The extracts showed higher inhibition against α-glucosidase (7.32-11.99 mmol acarbose equivalent [ACAE]/g extract) compared to α-amylase (0.51-0.55 mmol ACAE/g extract). SC extract was the most active (137.54 mg kojic acid equivalent/g extract) tyrosinase inhibitor and FV extract (113.75 mg Orlistat equivalent/g) the best lipase inhibitor. SM extract showed potent antibacterial effect against B. cereus (MIC (minimum inhibitory concentration) 0.180 mg/mL), P. mirabilis (MIC 0.180 mg/mL), M. flavus (MIC 0.560 mg/mL), P. aeruginosa (MIC 0.275 mg/mL), and S. typhimurium (MIC 1.500 mg/mL). FV extract (MIC 0.140 mg/mL) suppressed A. fumigatus growth. Cytotoxicity was assessed on murine macrophage (RAW 264.7), human embryonic kidney (HEK 293), and human hepatocellular carcinoma (HepG2) cell lines. FV (60.3%) and SM (57.4%) showed the highest reduction on RAW 264.7 cellular viability, whereas SM (74.1%) showed toxicity against HepG2. This study supports that the Apiaceae species could be considered as promising candidates for the development of novel pharmacophores for the management of several human ailments.",
journal = "Industrial Crops and Products",
title = "Phytochemical characterization and bioactivities of five Apiaceae species: Natural sources for novel ingredients",
volume = "135",
doi = "10.1016/J.INDCROP.2019.04.033",
pages = "107-121"
}

Zengin, G., Mahomoodally, M. F., Paksoy, M. Y., Picot-Allain, C., Glamočlija, J., Soković, M., Diuzheva, A., Jekő, J., Cziáky, Z., Rodrigues, M. J., Sinan, K. I.,& Custodio, L.. (2019). Phytochemical characterization and bioactivities of five Apiaceae species: Natural sources for novel ingredients. in Industrial Crops and Products, 135, 107-121.
https://doi.org/10.1016/J.INDCROP.2019.04.033

Zengin G, Mahomoodally MF, Paksoy MY, Picot-Allain C, Glamočlija J, Soković M, Diuzheva A, Jekő J, Cziáky Z, Rodrigues MJ, Sinan KI, Custodio L. Phytochemical characterization and bioactivities of five Apiaceae species: Natural sources for novel ingredients. in Industrial Crops and Products. 2019;135:107-121.
doi:10.1016/J.INDCROP.2019.04.033 .

Zengin, Gokhan, Mahomoodally, Mohamad Fawzi, Paksoy, Mehmet Yavuz, Picot-Allain, Carene, Glamočlija, Jasmina, Soković, Marina, Diuzheva, Alina, Jekő, József, Cziáky, Zoltán, Rodrigues, Maria João, Sinan, Kouadio Ibrahime, Custodio, Luisa, "Phytochemical characterization and bioactivities of five Apiaceae species: Natural sources for novel ingredients" in Industrial Crops and Products, 135 (2019):107-121,
https://doi.org/10.1016/J.INDCROP.2019.04.033 . .