TY  - JOUR
AU  - Radulović, Olga
AU  - Petrić, Marija
AU  - Raspor, Martin
AU  - Stanojević, Olja
AU  - Janakiev, Tamara
AU  - Tadić, Vojin
AU  - Stanković, Slaviša
PY  - 2019
UR  - http://www.pjoes.com/doi/10.15244/pjoes/81687
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3255
AB  - In this work, we demonstrate that the rhizosphere of common duckweed (Lemna minor) is inhabited with various phenol-resistant bacterial strains. Based on 16S rRNA sequencing, we have identified 60 rhizosphere-associated bacterial isolates belonging to 10 different bacterial genera (Pseudomonas, Hafnia, Serratia, Enterobacter, Micrococcus, Stenotrophomonas, Xanthomonas, Bacillus, Staphylococcus and Klebsiella). All isolates have been tested for phenol resistance and ability to utilize phenol as the sole carbon source. 70% of all isolates survived high doses of phenol (≥200 mg/L) and at least 27% can be potentially acclimatized by gradual increase of phenol concentration. Finally, based on high phenol resistance, ability to utilize phenol as the sole carbon source and documented low pathogenicity, we propose 5 strains as potentially excellent candidates for bioremediation. These 5 strains taxonomically correspond to Klebsiella sp., Serratia sp., and Hafnia sp., respectively. To the best of our knowledge, this is the first attempt to assess decontamination capacity of Serratia nematodiphila and Hafnia sp. in the context of bioremediation of phenol-contaminated aqueous media. Although additional analyses are needed, interaction between the common duckweed and the selected bacterial strains may be utilized in future bioremediation strategies.
T2  - Polish Journal of Environmental Studies
T1  - Culture-Dependent Analysis of 16S rRNA Sequences Associated with the Rhizosphere of Lemna minor and Assessment of Bacterial Phenol-Resistance: Plant/Bacteria System for Potential Bioremediation – Part II
IS  - 2
VL  - 28
DO  - 10.15244/pjoes/81687
SP  - 811
EP  - 822
ER  - 

@article{
author = "Radulović, Olga and Petrić, Marija and Raspor, Martin and Stanojević, Olja and Janakiev, Tamara and Tadić, Vojin and Stanković, Slaviša",
year = "2019",
abstract = "In this work, we demonstrate that the rhizosphere of common duckweed (Lemna minor) is inhabited with various phenol-resistant bacterial strains. Based on 16S rRNA sequencing, we have identified 60 rhizosphere-associated bacterial isolates belonging to 10 different bacterial genera (Pseudomonas, Hafnia, Serratia, Enterobacter, Micrococcus, Stenotrophomonas, Xanthomonas, Bacillus, Staphylococcus and Klebsiella). All isolates have been tested for phenol resistance and ability to utilize phenol as the sole carbon source. 70% of all isolates survived high doses of phenol (≥200 mg/L) and at least 27% can be potentially acclimatized by gradual increase of phenol concentration. Finally, based on high phenol resistance, ability to utilize phenol as the sole carbon source and documented low pathogenicity, we propose 5 strains as potentially excellent candidates for bioremediation. These 5 strains taxonomically correspond to Klebsiella sp., Serratia sp., and Hafnia sp., respectively. To the best of our knowledge, this is the first attempt to assess decontamination capacity of Serratia nematodiphila and Hafnia sp. in the context of bioremediation of phenol-contaminated aqueous media. Although additional analyses are needed, interaction between the common duckweed and the selected bacterial strains may be utilized in future bioremediation strategies.",
journal = "Polish Journal of Environmental Studies",
title = "Culture-Dependent Analysis of 16S rRNA Sequences Associated with the Rhizosphere of Lemna minor and Assessment of Bacterial Phenol-Resistance: Plant/Bacteria System for Potential Bioremediation – Part II",
number = "2",
volume = "28",
doi = "10.15244/pjoes/81687",
pages = "811-822"
}

Radulović, O., Petrić, M., Raspor, M., Stanojević, O., Janakiev, T., Tadić, V.,& Stanković, S.. (2019). Culture-Dependent Analysis of 16S rRNA Sequences Associated with the Rhizosphere of Lemna minor and Assessment of Bacterial Phenol-Resistance: Plant/Bacteria System for Potential Bioremediation – Part II. in Polish Journal of Environmental Studies, 28(2), 811-822.
https://doi.org/10.15244/pjoes/81687

Radulović O, Petrić M, Raspor M, Stanojević O, Janakiev T, Tadić V, Stanković S. Culture-Dependent Analysis of 16S rRNA Sequences Associated with the Rhizosphere of Lemna minor and Assessment of Bacterial Phenol-Resistance: Plant/Bacteria System for Potential Bioremediation – Part II. in Polish Journal of Environmental Studies. 2019;28(2):811-822.
doi:10.15244/pjoes/81687 .

Radulović, Olga, Petrić, Marija, Raspor, Martin, Stanojević, Olja, Janakiev, Tamara, Tadić, Vojin, Stanković, Slaviša, "Culture-Dependent Analysis of 16S rRNA Sequences Associated with the Rhizosphere of Lemna minor and Assessment of Bacterial Phenol-Resistance: Plant/Bacteria System for Potential Bioremediation – Part II" in Polish Journal of Environmental Studies, 28, no. 2 (2019):811-822,
https://doi.org/10.15244/pjoes/81687 . .

TY  - JOUR
AU  - Radulović, Olga
AU  - Petrić, Marija
AU  - Raspor, Martin
AU  - Tadić, Vojin
AU  - Jovanović, Petar
AU  - Zečević, Vladan
PY  - 2019
UR  - http://www.journalssystem.com/pjoes/Assessment-of-in-vitro-multiplication-of-Lemna-minor-in-the-presence-of-phenol-plant,84921,0,2.html
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3251
AB  - The aim of this work was to examine the multiplication of the common duckweed (Lemna minor), an aquatic plant species widespread in European stagnant waters, in two different media (Murashige – Skoog and Hoagland) with and without phenol supplementation. In order to quantify plant multiplication we have used relative growth rate and tolerance indices on both tested media and at five phenol concentrations (10, 15, 20, 30 and 100 mg/L). Furthermore, we examined the possibility of phenol removal from aqueous media containing different phenol concentrations, by using plant/bacteria system consisting of the duckweed and its naturally occurring microbial populations. After 7 days, number of newly formed fronds was approximately four times higher than at the beginning of the experiment on both tested media. The most important result in this study was removal of 70% of phenol from the highest initial concentration of 100 mg/L, in mixed cultures of duckweed and bacteria. By comparison, aseptic duckweed cultures removed approximately 50% of phenol at the same initial concentration. Our duckweed specimen showed a fast reproduction rate, high tolerance to phenol and a possible cooperation with rhizosphere-associated bacteria. All of these traits can be ultimately utilized for bioremediation purposes.
T2  - Polish Journal of Environmental Studies
T1  - Assessment of in vitro Multiplication of Lemna minor in the Presence of Phenol: Plant/Bacteria System for Potential Bioremediation – Part I
IS  - 2
VL  - 28
DO  - 10.15244/pjoes/84921
SP  - 803
EP  - 809
ER  - 

@article{
author = "Radulović, Olga and Petrić, Marija and Raspor, Martin and Tadić, Vojin and Jovanović, Petar and Zečević, Vladan",
year = "2019",
abstract = "The aim of this work was to examine the multiplication of the common duckweed (Lemna minor), an aquatic plant species widespread in European stagnant waters, in two different media (Murashige – Skoog and Hoagland) with and without phenol supplementation. In order to quantify plant multiplication we have used relative growth rate and tolerance indices on both tested media and at five phenol concentrations (10, 15, 20, 30 and 100 mg/L). Furthermore, we examined the possibility of phenol removal from aqueous media containing different phenol concentrations, by using plant/bacteria system consisting of the duckweed and its naturally occurring microbial populations. After 7 days, number of newly formed fronds was approximately four times higher than at the beginning of the experiment on both tested media. The most important result in this study was removal of 70% of phenol from the highest initial concentration of 100 mg/L, in mixed cultures of duckweed and bacteria. By comparison, aseptic duckweed cultures removed approximately 50% of phenol at the same initial concentration. Our duckweed specimen showed a fast reproduction rate, high tolerance to phenol and a possible cooperation with rhizosphere-associated bacteria. All of these traits can be ultimately utilized for bioremediation purposes.",
journal = "Polish Journal of Environmental Studies",
title = "Assessment of in vitro Multiplication of Lemna minor in the Presence of Phenol: Plant/Bacteria System for Potential Bioremediation – Part I",
number = "2",
volume = "28",
doi = "10.15244/pjoes/84921",
pages = "803-809"
}

Radulović, O., Petrić, M., Raspor, M., Tadić, V., Jovanović, P.,& Zečević, V.. (2019). Assessment of in vitro Multiplication of Lemna minor in the Presence of Phenol: Plant/Bacteria System for Potential Bioremediation – Part I. in Polish Journal of Environmental Studies, 28(2), 803-809.
https://doi.org/10.15244/pjoes/84921

Radulović O, Petrić M, Raspor M, Tadić V, Jovanović P, Zečević V. Assessment of in vitro Multiplication of Lemna minor in the Presence of Phenol: Plant/Bacteria System for Potential Bioremediation – Part I. in Polish Journal of Environmental Studies. 2019;28(2):803-809.
doi:10.15244/pjoes/84921 .

Radulović, Olga, Petrić, Marija, Raspor, Martin, Tadić, Vojin, Jovanović, Petar, Zečević, Vladan, "Assessment of in vitro Multiplication of Lemna minor in the Presence of Phenol: Plant/Bacteria System for Potential Bioremediation – Part I" in Polish Journal of Environmental Studies, 28, no. 2 (2019):803-809,
https://doi.org/10.15244/pjoes/84921 . .

TY  - JOUR
AU  - Tadić, Vojin
AU  - Petrić, Marija
AU  - Uzelac, Branka
AU  - Milošević, Snežana
AU  - Vujčić, Zoran
AU  - Stevanović, Jasmina
AU  - Tadić, Jovan
PY  - 2018
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0304423817307483
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2956
AB  - We investigated the removal of phenol from water solutions (200 mg L−1) using two varieties of lettuce (Lactuca sativa L.) and their hairy roots. Experiments were done in a hydroponic system where adult plants were grown in phenol solutions for 10 days. The solution was refreshed every two days in order to maintain the constant concentration of phenol. Hairy roots were also cultivated in a solution containing phenol at concentrations varying from 25 to 125 mg L−1 in order to determine the maximum concentration of phenol that can be removed by hairy roots. Both varieties of lettuce reduced the concentration of phenol below the detection limit after six days at the initial phenol concentration of 200 mg L−1. Transformed roots completely removed phenol at the initial concentrations of 100 mg L−1, but were not able to remove phenol at constant concentration above 25 mg L−1. Lettuce plants and hairy roots are excellent candidates for the process of phenol removal from wastewaters. This plant is good choice for bioremediation of water and represents a potentially efficient and inexpensive system for water purification. The performance of lettuce plants and hairy roots to remove phenol from water solutions under real conditions, depleted nutrients or presence of other compounds should be examined further.
T2  - Scientia Horticulturae
T1  - Phenol removal from solution using different varieties of lettuce ( Lactuca sativa L.) – Part 1
VL  - 231
DO  - 10.1016/j.scienta.2017.12.025
SP  - 210
EP  - 218
ER  - 

@article{
author = "Tadić, Vojin and Petrić, Marija and Uzelac, Branka and Milošević, Snežana and Vujčić, Zoran and Stevanović, Jasmina and Tadić, Jovan",
year = "2018",
abstract = "We investigated the removal of phenol from water solutions (200 mg L−1) using two varieties of lettuce (Lactuca sativa L.) and their hairy roots. Experiments were done in a hydroponic system where adult plants were grown in phenol solutions for 10 days. The solution was refreshed every two days in order to maintain the constant concentration of phenol. Hairy roots were also cultivated in a solution containing phenol at concentrations varying from 25 to 125 mg L−1 in order to determine the maximum concentration of phenol that can be removed by hairy roots. Both varieties of lettuce reduced the concentration of phenol below the detection limit after six days at the initial phenol concentration of 200 mg L−1. Transformed roots completely removed phenol at the initial concentrations of 100 mg L−1, but were not able to remove phenol at constant concentration above 25 mg L−1. Lettuce plants and hairy roots are excellent candidates for the process of phenol removal from wastewaters. This plant is good choice for bioremediation of water and represents a potentially efficient and inexpensive system for water purification. The performance of lettuce plants and hairy roots to remove phenol from water solutions under real conditions, depleted nutrients or presence of other compounds should be examined further.",
journal = "Scientia Horticulturae",
title = "Phenol removal from solution using different varieties of lettuce ( Lactuca sativa L.) – Part 1",
volume = "231",
doi = "10.1016/j.scienta.2017.12.025",
pages = "210-218"
}

Tadić, V., Petrić, M., Uzelac, B., Milošević, S., Vujčić, Z., Stevanović, J.,& Tadić, J.. (2018). Phenol removal from solution using different varieties of lettuce ( Lactuca sativa L.) – Part 1. in Scientia Horticulturae, 231, 210-218.
https://doi.org/10.1016/j.scienta.2017.12.025

Tadić V, Petrić M, Uzelac B, Milošević S, Vujčić Z, Stevanović J, Tadić J. Phenol removal from solution using different varieties of lettuce ( Lactuca sativa L.) – Part 1. in Scientia Horticulturae. 2018;231:210-218.
doi:10.1016/j.scienta.2017.12.025 .

Tadić, Vojin, Petrić, Marija, Uzelac, Branka, Milošević, Snežana, Vujčić, Zoran, Stevanović, Jasmina, Tadić, Jovan, "Phenol removal from solution using different varieties of lettuce ( Lactuca sativa L.) – Part 1" in Scientia Horticulturae, 231 (2018):210-218,
https://doi.org/10.1016/j.scienta.2017.12.025 . .

TY  - JOUR
AU  - Tadić, Vojin
AU  - Tadić, Jovan
AU  - Milošević, Snežana
AU  - Cingel, Aleksandar
AU  - Prodanović, Olivera
AU  - Ćosić, Tatjana
AU  - Vujčić, Zoran
PY  - 2018
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0304423817307471
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2955
AB  - In this study we investigated physiological parameters of stress (enzymatic and non-enzymatic) in lettuce (Lactuca sativa L.) and its hairy roots induced by water solution of phenol. Two varieties of lettuce were examined, Ljubljanska ledenka and Nansen. Plants were grown in water with phenol concentration of 200 mgL−1 during 10 days. We monitored activity of peroxidases, catalases, polyphenol oxidase and superoxide dismutase, as well as proline and chlorophyll content. We observed a decrease in peroxidases, and increase in activity of catalase, polyphenol oxidase and superoxide dismutase compared to control plants. The concentration of proline was constantly increasing in both lettuce varieties over the course of the experiment. We detected an increase in activity of all monitored enzymes, except polyphenol oxidases, in hairy roots. The hydroponic system provides a useful framework for studying the effect of different harmful substances and its elimination. In such a system, as used in this work for the study of physiological processes in antioxidant protection activated when plant was exposed to phenol, lettuce and its hairy roots can be viewed as tools for water remediation.
T2  - Scientia Horticulturae
T1  - Phenol induced physiological stress in hydroponically grown lettuce ( Lactuca sativa L.)— Part 2
VL  - 232
DO  - 10.1016/j.scienta.2017.12.024
SP  - 71
EP  - 83
ER  - 

@article{
author = "Tadić, Vojin and Tadić, Jovan and Milošević, Snežana and Cingel, Aleksandar and Prodanović, Olivera and Ćosić, Tatjana and Vujčić, Zoran",
year = "2018",
abstract = "In this study we investigated physiological parameters of stress (enzymatic and non-enzymatic) in lettuce (Lactuca sativa L.) and its hairy roots induced by water solution of phenol. Two varieties of lettuce were examined, Ljubljanska ledenka and Nansen. Plants were grown in water with phenol concentration of 200 mgL−1 during 10 days. We monitored activity of peroxidases, catalases, polyphenol oxidase and superoxide dismutase, as well as proline and chlorophyll content. We observed a decrease in peroxidases, and increase in activity of catalase, polyphenol oxidase and superoxide dismutase compared to control plants. The concentration of proline was constantly increasing in both lettuce varieties over the course of the experiment. We detected an increase in activity of all monitored enzymes, except polyphenol oxidases, in hairy roots. The hydroponic system provides a useful framework for studying the effect of different harmful substances and its elimination. In such a system, as used in this work for the study of physiological processes in antioxidant protection activated when plant was exposed to phenol, lettuce and its hairy roots can be viewed as tools for water remediation.",
journal = "Scientia Horticulturae",
title = "Phenol induced physiological stress in hydroponically grown lettuce ( Lactuca sativa L.)— Part 2",
volume = "232",
doi = "10.1016/j.scienta.2017.12.024",
pages = "71-83"
}

Tadić, V., Tadić, J., Milošević, S., Cingel, A., Prodanović, O., Ćosić, T.,& Vujčić, Z.. (2018). Phenol induced physiological stress in hydroponically grown lettuce ( Lactuca sativa L.)— Part 2. in Scientia Horticulturae, 232, 71-83.
https://doi.org/10.1016/j.scienta.2017.12.024

Tadić V, Tadić J, Milošević S, Cingel A, Prodanović O, Ćosić T, Vujčić Z. Phenol induced physiological stress in hydroponically grown lettuce ( Lactuca sativa L.)— Part 2. in Scientia Horticulturae. 2018;232:71-83.
doi:10.1016/j.scienta.2017.12.024 .

Tadić, Vojin, Tadić, Jovan, Milošević, Snežana, Cingel, Aleksandar, Prodanović, Olivera, Ćosić, Tatjana, Vujčić, Zoran, "Phenol induced physiological stress in hydroponically grown lettuce ( Lactuca sativa L.)— Part 2" in Scientia Horticulturae, 232 (2018):71-83,
https://doi.org/10.1016/j.scienta.2017.12.024 . .

TY  - JOUR
AU  - Petrić, Marija
AU  - Subotić, Angelina
AU  - Jevremović, Slađana
AU  - Trifunović-Momčilov, Milana
AU  - Tadić, Vojin
AU  - Grujić, Marica
AU  - Vujčić, Zoran
PY  - 2017
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641700007P
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/1279
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2883
AB  - The aim of this study was to determine the enzymatic profile of esterases and peroxidases during early stages of somatic embryogenesis of Fritillaria meleagris L. Somatic embryogenesis was induced using the leaf base as explant on a medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D). Zymography showed the presence of different moieties, six isoforms of esterases and peroxidases, during morphogenesis as compared to control explants. One isoform of esterases was detected only during the process of somatic embryogenesis, and one isoform was detected in control explants. Analysis of esterases with 1-naphthyl butyrate proved that esterases, which participate in somatic embryogenesis of F. meleagris, belong to the family of aryl esterases. For the first time it was proved that five isoforms of esterases, which are involved in morphogenesis of F. meleagris, belong to the family of aryl esterases, while two isoforms are carboxyl esterases. One isoform of carboxyl esterases was visible in control explants. This is also the first description of peroxidases during the morphogenetic process, and of the difference between aryl and carboxyl esterases. More isoforms of esterases during morphogenesis as compared to control explants are probably responsible for some early physiological process during somatic embryogenesis of F. meleagris.
T2  - Archives of Biological Sciences
T1  - Esterase and peroxidase isoforms during initial stages of somatic embryogenesis in Fritillaria meleagris L. leaf base
IS  - 4
VL  - 69
DO  - 10.2298/ABS161130007P
SP  - 619
EP  - 625
ER  - 

@article{
author = "Petrić, Marija and Subotić, Angelina and Jevremović, Slađana and Trifunović-Momčilov, Milana and Tadić, Vojin and Grujić, Marica and Vujčić, Zoran",
year = "2017",
abstract = "The aim of this study was to determine the enzymatic profile of esterases and peroxidases during early stages of somatic embryogenesis of Fritillaria meleagris L. Somatic embryogenesis was induced using the leaf base as explant on a medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D). Zymography showed the presence of different moieties, six isoforms of esterases and peroxidases, during morphogenesis as compared to control explants. One isoform of esterases was detected only during the process of somatic embryogenesis, and one isoform was detected in control explants. Analysis of esterases with 1-naphthyl butyrate proved that esterases, which participate in somatic embryogenesis of F. meleagris, belong to the family of aryl esterases. For the first time it was proved that five isoforms of esterases, which are involved in morphogenesis of F. meleagris, belong to the family of aryl esterases, while two isoforms are carboxyl esterases. One isoform of carboxyl esterases was visible in control explants. This is also the first description of peroxidases during the morphogenetic process, and of the difference between aryl and carboxyl esterases. More isoforms of esterases during morphogenesis as compared to control explants are probably responsible for some early physiological process during somatic embryogenesis of F. meleagris.",
journal = "Archives of Biological Sciences",
title = "Esterase and peroxidase isoforms during initial stages of somatic embryogenesis in Fritillaria meleagris L. leaf base",
number = "4",
volume = "69",
doi = "10.2298/ABS161130007P",
pages = "619-625"
}

Petrić, M., Subotić, A., Jevremović, S., Trifunović-Momčilov, M., Tadić, V., Grujić, M.,& Vujčić, Z.. (2017). Esterase and peroxidase isoforms during initial stages of somatic embryogenesis in Fritillaria meleagris L. leaf base. in Archives of Biological Sciences, 69(4), 619-625.
https://doi.org/10.2298/ABS161130007P

Petrić M, Subotić A, Jevremović S, Trifunović-Momčilov M, Tadić V, Grujić M, Vujčić Z. Esterase and peroxidase isoforms during initial stages of somatic embryogenesis in Fritillaria meleagris L. leaf base. in Archives of Biological Sciences. 2017;69(4):619-625.
doi:10.2298/ABS161130007P .

Petrić, Marija, Subotić, Angelina, Jevremović, Slađana, Trifunović-Momčilov, Milana, Tadić, Vojin, Grujić, Marica, Vujčić, Zoran, "Esterase and peroxidase isoforms during initial stages of somatic embryogenesis in Fritillaria meleagris L. leaf base" in Archives of Biological Sciences, 69, no. 4 (2017):619-625,
https://doi.org/10.2298/ABS161130007P . .

TY  - JOUR
AU  - Petrić, Marija P
AU  - Jevremović, Slađana
AU  - Trifunović-Momčilov, Milana
AU  - Tadić, Vojin
AU  - Milošević, Snežana
AU  - Dragičević, Milan B.
AU  - Subotić, Angelina
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/956
AB  - We investigated the effect of low temperature and gibberellic acid (GA(3)) treatment on dormancy in Fritillaria meleagris L. bulbs. Also, we studied the effect of dormancy breaking on the antioxidant enzymes activity. To overcome dormancy, bulbs require a period (4-8 weeks) of exposure to low temperature. Bulbs regenerated in vitro were grown in the dark on medium without growth regulators at the standard (24 A degrees C) or at low temperatures (4 and 15 A degrees C) for 4, 6, 8 and 10 weeks. Bulbs were collected after 3, 4 and 5 weeks of cooling at 4 A degrees C. To investigate the influence of GA(3) on dormancy, bulbs were treated for 24 h with GA(3) solutions with 1, 2 and 3 mg l(-1) concentrations. During the period of growth of bulbs at 4 A degrees C, regeneration of bulbs was very weak, while at 15 A degrees C the number of regenerated bulbs increased significantly. Improved bulb sprouting was achieved by a short treatment with gibberellin. Low temperature also represents a kind of oxidative stress for the plant. The activity of superoxide dismutase, catalase (CAT) and peroxidase (POX) in bulbs of F. meleagris L. grown in vitro and ex vitro increased with decreasing temperature in contrast to glutathione reductase. POX showed generally lower activity than CAT which indicates that major role in the breaking dormancy and preparing bulbs for sprouting have catalases.
T2  - Acta Physiologiae Plantarum
T1  - The effect of low temperature and GA(3) treatments on dormancy breaking and activity of antioxidant enzymes in Fritillaria meleagris bulblets cultured in vitro
IS  - 11
VL  - 35
SP  - 23
EP  - 3236
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_956
ER  - 

@article{
author = "Petrić, Marija P and Jevremović, Slađana and Trifunović-Momčilov, Milana and Tadić, Vojin and Milošević, Snežana and Dragičević, Milan B. and Subotić, Angelina",
year = "2013",
abstract = "We investigated the effect of low temperature and gibberellic acid (GA(3)) treatment on dormancy in Fritillaria meleagris L. bulbs. Also, we studied the effect of dormancy breaking on the antioxidant enzymes activity. To overcome dormancy, bulbs require a period (4-8 weeks) of exposure to low temperature. Bulbs regenerated in vitro were grown in the dark on medium without growth regulators at the standard (24 A degrees C) or at low temperatures (4 and 15 A degrees C) for 4, 6, 8 and 10 weeks. Bulbs were collected after 3, 4 and 5 weeks of cooling at 4 A degrees C. To investigate the influence of GA(3) on dormancy, bulbs were treated for 24 h with GA(3) solutions with 1, 2 and 3 mg l(-1) concentrations. During the period of growth of bulbs at 4 A degrees C, regeneration of bulbs was very weak, while at 15 A degrees C the number of regenerated bulbs increased significantly. Improved bulb sprouting was achieved by a short treatment with gibberellin. Low temperature also represents a kind of oxidative stress for the plant. The activity of superoxide dismutase, catalase (CAT) and peroxidase (POX) in bulbs of F. meleagris L. grown in vitro and ex vitro increased with decreasing temperature in contrast to glutathione reductase. POX showed generally lower activity than CAT which indicates that major role in the breaking dormancy and preparing bulbs for sprouting have catalases.",
journal = "Acta Physiologiae Plantarum",
title = "The effect of low temperature and GA(3) treatments on dormancy breaking and activity of antioxidant enzymes in Fritillaria meleagris bulblets cultured in vitro",
number = "11",
volume = "35",
pages = "23-3236",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_956"
}

Petrić, M. P., Jevremović, S., Trifunović-Momčilov, M., Tadić, V., Milošević, S., Dragičević, M. B.,& Subotić, A.. (2013). The effect of low temperature and GA(3) treatments on dormancy breaking and activity of antioxidant enzymes in Fritillaria meleagris bulblets cultured in vitro. in Acta Physiologiae Plantarum, 35(11), 23-3236.
https://hdl.handle.net/21.15107/rcub_ibiss_956

Petrić MP, Jevremović S, Trifunović-Momčilov M, Tadić V, Milošević S, Dragičević MB, Subotić A. The effect of low temperature and GA(3) treatments on dormancy breaking and activity of antioxidant enzymes in Fritillaria meleagris bulblets cultured in vitro. in Acta Physiologiae Plantarum. 2013;35(11):23-3236.
https://hdl.handle.net/21.15107/rcub_ibiss_956 .

Petrić, Marija P, Jevremović, Slađana, Trifunović-Momčilov, Milana, Tadić, Vojin, Milošević, Snežana, Dragičević, Milan B., Subotić, Angelina, "The effect of low temperature and GA(3) treatments on dormancy breaking and activity of antioxidant enzymes in Fritillaria meleagris bulblets cultured in vitro" in Acta Physiologiae Plantarum, 35, no. 11 (2013):23-3236,
https://hdl.handle.net/21.15107/rcub_ibiss_956 .

TY  - JOUR
AU  - Milošević, Snežana
AU  - Cingel, Aleksandar
AU  - Ninković, Slavica
AU  - Nikolić, Dragana
AU  - Jevremović, Slađana
AU  - Tadić, Vojin
AU  - Subotić, Angelina
PY  - 2011
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/366
AB  - One of the leading areas of modern biotechnology is to develop plants resistant to pathogens using genetic engineering methods. The genetic transformation of I. hawkerii Bull. with Agrobacterium tumefaciens C58C1pac1 was observe in this study, and the aim was to derive plants carrying the gene for pac1 resistance to tomato spotted wilt virus and other plant RNA viruses. I. hawkerii Bull. transformants on a selective medium rapidly failed, and not a single clone survived the fifth passage. This decline was due to great susceptibility of this type to kanamycin or failure of the transformation.
AB  - Jedna od vodećih oblasti savremene biotehnologije je dobijanje biljaka otpornih na patogene pomoću metoda genetičkog inženjeringa. U ovom radu je proučavana genetička transformacija Impatiens hawkerii Bull. pomoću Agrobacterium tumefaciens C58C1pac1, a cilj je dobijanje biljaka koje nose pac1 gen za rezistentnost na virus bronzavosti paradajza i druge biljne RNK viruse. Transformanati I. hawkerii Bull. na selektivnoj podlozi su brzo propadali, a ni jedan klon nije preživeo peti pasaž. Ovo propadanje je posledica velike osetljivosti ove vrste na kanamicin ili neuspešnosti transfomacije.
T2  - Journal of Scientific Agricultural Research
T1  - Uspostavljanje model sistema za transformaciju biljaka I. hawkerii Bull.
T1  - Establishing a model system for transformation of I. hawkerii Bull. plants
IS  - 1
VL  - 72
SP  - 23
EP  - 27
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_366
ER  - 

@article{
author = "Milošević, Snežana and Cingel, Aleksandar and Ninković, Slavica and Nikolić, Dragana and Jevremović, Slađana and Tadić, Vojin and Subotić, Angelina",
year = "2011, 2011",
abstract = "One of the leading areas of modern biotechnology is to develop plants resistant to pathogens using genetic engineering methods. The genetic transformation of I. hawkerii Bull. with Agrobacterium tumefaciens C58C1pac1 was observe in this study, and the aim was to derive plants carrying the gene for pac1 resistance to tomato spotted wilt virus and other plant RNA viruses. I. hawkerii Bull. transformants on a selective medium rapidly failed, and not a single clone survived the fifth passage. This decline was due to great susceptibility of this type to kanamycin or failure of the transformation., Jedna od vodećih oblasti savremene biotehnologije je dobijanje biljaka otpornih na patogene pomoću metoda genetičkog inženjeringa. U ovom radu je proučavana genetička transformacija Impatiens hawkerii Bull. pomoću Agrobacterium tumefaciens C58C1pac1, a cilj je dobijanje biljaka koje nose pac1 gen za rezistentnost na virus bronzavosti paradajza i druge biljne RNK viruse. Transformanati I. hawkerii Bull. na selektivnoj podlozi su brzo propadali, a ni jedan klon nije preživeo peti pasaž. Ovo propadanje je posledica velike osetljivosti ove vrste na kanamicin ili neuspešnosti transfomacije.",
journal = "Journal of Scientific Agricultural Research",
title = "Uspostavljanje model sistema za transformaciju biljaka I. hawkerii Bull., Establishing a model system for transformation of I. hawkerii Bull. plants",
number = "1",
volume = "72",
pages = "23-27",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_366"
}

Milošević, S., Cingel, A., Ninković, S., Nikolić, D., Jevremović, S., Tadić, V.,& Subotić, A.. (2011). Uspostavljanje model sistema za transformaciju biljaka I. hawkerii Bull.. in Journal of Scientific Agricultural Research, 72(1), 23-27.
https://hdl.handle.net/21.15107/rcub_ibiss_366

Milošević S, Cingel A, Ninković S, Nikolić D, Jevremović S, Tadić V, Subotić A. Uspostavljanje model sistema za transformaciju biljaka I. hawkerii Bull.. in Journal of Scientific Agricultural Research. 2011;72(1):23-27.
https://hdl.handle.net/21.15107/rcub_ibiss_366 .

Milošević, Snežana, Cingel, Aleksandar, Ninković, Slavica, Nikolić, Dragana, Jevremović, Slađana, Tadić, Vojin, Subotić, Angelina, "Uspostavljanje model sistema za transformaciju biljaka I. hawkerii Bull." in Journal of Scientific Agricultural Research, 72, no. 1 (2011):23-27,
https://hdl.handle.net/21.15107/rcub_ibiss_366 .

TY  - JOUR
AU  - Milošević, Snežana
AU  - Cingel, Aleksandar
AU  - Ninković, Slavica
AU  - Nikolić, Dragana
AU  - Jevremović, Slađana
AU  - Tadić, Vojin
AU  - Subotić, Angelina
PY  - 2011
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/365
AB  - In order to detect and identify viruses in the plant material or to confirm the expression of a gene after genetic transformation it is necessary to have a fast, simple and reliable method for the extraction of DNA and RNA. In this study, we tested a protocol for DNA and three protocols for RNA extraction from plants of I. walleriana L. RNA obtained by different extraction methods significantly differed in its yield and purity. The optimum method proved to be the one applicable to plant species that contain large amounts of secondary metabolites (polyphenols and polysaccharides). Nucleic acid extracted by the application of these methods showed satisfactory results in PCR and RT-PCR analysis.
AB  - Za detekciju i identifikaciju virusa u biljnom materijalu ili potvrdu ekspresije nekog gena nakon genetičkih transformacija neophodno je imati brzu, jednostavnu i pouzdanu metodu za ekstrakciju DNK i RNK. U ovom radu smo testirali jedan protokol za izolaciju DNK i tri za ekstrakciju RNK iz biljaka Impatiens walleriana L. RNK dobijena primenom različitih metoda za ekstrakciju znatno se razlikovala po prinosu i čistoći. Kao optimalna metoda se pokazala ona koja se primenjuje za biljne vrste koje sadrže velike količine sekundarnih metabolita (polifenola i polisaharida). Nukleinske kiseline ekstrahovane primenom ovih metoda pokazale su zadovoljavajuće rezultate u PCR i RT-PCR analizama.
T2  - Journal of Scientific Agricultural Research
T1  - Protokol za ekstrakciju DNK i poređenje tri protokola za ekstrakciju RNK iz biljaka I. walleriana L.
T1  - A rapid protocol for DNA extraction and a comparison of tree protocols for RNA extraction from Impatiens walleriana L.
IS  - 1
VL  - 72
SP  - 15
EP  - 21
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_365
ER  - 

@article{
author = "Milošević, Snežana and Cingel, Aleksandar and Ninković, Slavica and Nikolić, Dragana and Jevremović, Slađana and Tadić, Vojin and Subotić, Angelina",
year = "2011, 2011",
abstract = "In order to detect and identify viruses in the plant material or to confirm the expression of a gene after genetic transformation it is necessary to have a fast, simple and reliable method for the extraction of DNA and RNA. In this study, we tested a protocol for DNA and three protocols for RNA extraction from plants of I. walleriana L. RNA obtained by different extraction methods significantly differed in its yield and purity. The optimum method proved to be the one applicable to plant species that contain large amounts of secondary metabolites (polyphenols and polysaccharides). Nucleic acid extracted by the application of these methods showed satisfactory results in PCR and RT-PCR analysis., Za detekciju i identifikaciju virusa u biljnom materijalu ili potvrdu ekspresije nekog gena nakon genetičkih transformacija neophodno je imati brzu, jednostavnu i pouzdanu metodu za ekstrakciju DNK i RNK. U ovom radu smo testirali jedan protokol za izolaciju DNK i tri za ekstrakciju RNK iz biljaka Impatiens walleriana L. RNK dobijena primenom različitih metoda za ekstrakciju znatno se razlikovala po prinosu i čistoći. Kao optimalna metoda se pokazala ona koja se primenjuje za biljne vrste koje sadrže velike količine sekundarnih metabolita (polifenola i polisaharida). Nukleinske kiseline ekstrahovane primenom ovih metoda pokazale su zadovoljavajuće rezultate u PCR i RT-PCR analizama.",
journal = "Journal of Scientific Agricultural Research",
title = "Protokol za ekstrakciju DNK i poređenje tri protokola za ekstrakciju RNK iz biljaka I. walleriana L., A rapid protocol for DNA extraction and a comparison of tree protocols for RNA extraction from Impatiens walleriana L.",
number = "1",
volume = "72",
pages = "15-21",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_365"
}

Milošević, S., Cingel, A., Ninković, S., Nikolić, D., Jevremović, S., Tadić, V.,& Subotić, A.. (2011). Protokol za ekstrakciju DNK i poređenje tri protokola za ekstrakciju RNK iz biljaka I. walleriana L.. in Journal of Scientific Agricultural Research, 72(1), 15-21.
https://hdl.handle.net/21.15107/rcub_ibiss_365

Milošević S, Cingel A, Ninković S, Nikolić D, Jevremović S, Tadić V, Subotić A. Protokol za ekstrakciju DNK i poređenje tri protokola za ekstrakciju RNK iz biljaka I. walleriana L.. in Journal of Scientific Agricultural Research. 2011;72(1):15-21.
https://hdl.handle.net/21.15107/rcub_ibiss_365 .

Milošević, Snežana, Cingel, Aleksandar, Ninković, Slavica, Nikolić, Dragana, Jevremović, Slađana, Tadić, Vojin, Subotić, Angelina, "Protokol za ekstrakciju DNK i poređenje tri protokola za ekstrakciju RNK iz biljaka I. walleriana L." in Journal of Scientific Agricultural Research, 72, no. 1 (2011):15-21,
https://hdl.handle.net/21.15107/rcub_ibiss_365 .