TY  - JOUR
AU  - Matekalo, Dragana
AU  - Skorić, Marijana
AU  - Nikolić, Tijana
AU  - Novaković, Lazar
AU  - Lukić, Milana
AU  - Božunović, Jelena
AU  - Aničić, Neda
AU  - Filipović, Biljana
AU  - Mišić, Danijela
PY  - 2018
UR  - https://www.sciencedirect.com/science/article/pii/S0031942218303777?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3146
AB  - While bioactive properties of Centaurium erythraea Rafn secoiridoid glucosides (SG) are widely recognized, many aspects related to their biochemistry, metabolism and relationship to the overall plant physiology are not yet understood. Here we present for the first time an insight into the molecular background of organ-specific and genotype-dependent constitutive biosynthesis of secoiridoids in C. erythraea, by comparing chemical profiles and secoiridoid glucosides-related gene expression. Genes encoding enzymes for intermediate steps of secoiridoids biosynthesis up to secologanin have been identified by analysing transcriptomic data from C. erythraea leaves. Results suggest an organ-specific capacity for the production and accumulation of secoiridoid glucosides, and highlight leaves as the main biosynthesis site. They also point out that significant differences in SG content among various C. erythraea genotypes, are, at least partially, determined by different expression patterns of SG-related genes. The biosynthesis of SG in C. erythraea leaves is enhanced upon treatments with methyl jasmonate (MeJA), which causes reprogramming of SG-related gene expression, leading to an increased production of valuable bioactive compounds. The present study unveiled several rate-limiting genes (encoding GES, G8O, 8HGO, IS and 7DLGT) in SG biosynthesis. SLS and CPR are highlighted as important genes/enzymes that might regulate biosynthetic flux through SG pathway. Information gathered within this study will help us gain deeper insight into the SG metabolism and develop strategies for enhanced biosynthesis of specific secoiridoid glucosides in homologous or heterologous systems.
T2  - Phytochemistry
T1  - Organ-specific and genotype-dependent constitutive biosynthesis of secoiridoid glucosides in Centaurium erythraea Rafn, and its elicitation with methyl jasmonate.
VL  - 155
DO  - 10.1016/j.phytochem.2018.07.015
SP  - 69
EP  - 82
ER  - 

@article{
author = "Matekalo, Dragana and Skorić, Marijana and Nikolić, Tijana and Novaković, Lazar and Lukić, Milana and Božunović, Jelena and Aničić, Neda and Filipović, Biljana and Mišić, Danijela",
year = "2018",
abstract = "While bioactive properties of Centaurium erythraea Rafn secoiridoid glucosides (SG) are widely recognized, many aspects related to their biochemistry, metabolism and relationship to the overall plant physiology are not yet understood. Here we present for the first time an insight into the molecular background of organ-specific and genotype-dependent constitutive biosynthesis of secoiridoids in C. erythraea, by comparing chemical profiles and secoiridoid glucosides-related gene expression. Genes encoding enzymes for intermediate steps of secoiridoids biosynthesis up to secologanin have been identified by analysing transcriptomic data from C. erythraea leaves. Results suggest an organ-specific capacity for the production and accumulation of secoiridoid glucosides, and highlight leaves as the main biosynthesis site. They also point out that significant differences in SG content among various C. erythraea genotypes, are, at least partially, determined by different expression patterns of SG-related genes. The biosynthesis of SG in C. erythraea leaves is enhanced upon treatments with methyl jasmonate (MeJA), which causes reprogramming of SG-related gene expression, leading to an increased production of valuable bioactive compounds. The present study unveiled several rate-limiting genes (encoding GES, G8O, 8HGO, IS and 7DLGT) in SG biosynthesis. SLS and CPR are highlighted as important genes/enzymes that might regulate biosynthetic flux through SG pathway. Information gathered within this study will help us gain deeper insight into the SG metabolism and develop strategies for enhanced biosynthesis of specific secoiridoid glucosides in homologous or heterologous systems.",
journal = "Phytochemistry",
title = "Organ-specific and genotype-dependent constitutive biosynthesis of secoiridoid glucosides in Centaurium erythraea Rafn, and its elicitation with methyl jasmonate.",
volume = "155",
doi = "10.1016/j.phytochem.2018.07.015",
pages = "69-82"
}

Matekalo, D., Skorić, M., Nikolić, T., Novaković, L., Lukić, M., Božunović, J., Aničić, N., Filipović, B.,& Mišić, D.. (2018). Organ-specific and genotype-dependent constitutive biosynthesis of secoiridoid glucosides in Centaurium erythraea Rafn, and its elicitation with methyl jasmonate.. in Phytochemistry, 155, 69-82.
https://doi.org/10.1016/j.phytochem.2018.07.015

Matekalo D, Skorić M, Nikolić T, Novaković L, Lukić M, Božunović J, Aničić N, Filipović B, Mišić D. Organ-specific and genotype-dependent constitutive biosynthesis of secoiridoid glucosides in Centaurium erythraea Rafn, and its elicitation with methyl jasmonate.. in Phytochemistry. 2018;155:69-82.
doi:10.1016/j.phytochem.2018.07.015 .

Matekalo, Dragana, Skorić, Marijana, Nikolić, Tijana, Novaković, Lazar, Lukić, Milana, Božunović, Jelena, Aničić, Neda, Filipović, Biljana, Mišić, Danijela, "Organ-specific and genotype-dependent constitutive biosynthesis of secoiridoid glucosides in Centaurium erythraea Rafn, and its elicitation with methyl jasmonate." in Phytochemistry, 155 (2018):69-82,
https://doi.org/10.1016/j.phytochem.2018.07.015 . .

TY  - CONF
AU  - Lukić, Milana
AU  - Skorić, Marijana
AU  - Matekalo, Dragana
AU  - Božunović, Jelena
AU  - Aničić, Neda
AU  - Filipović, Biljana
AU  - Mišić, Danijela
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4290
AB  - Centaurium erythraea (centaury), a plant with a long tradition of medicinal use, contains bioactive
secoiridoid glucosides (SGs). The exogenous application of methyl jasmonate (MeJA) may
elicit a response similar to stress, and is used in the present study to induce SG accumulation and
expression of SGs’ biosynthesis associated genes in centaury’s aerial parts. UHPLC-MS/MS analyses
have indicated that the addition of 250 μM MeJA to the medium has caused overproduction of
almost all investigated secondary metabolites. The most notable change was detected for swertiamarin
production, which was several fold-higher in MeJA-treated plants. For the purpose of determining
the key genes of MeJA response, we performed a qPCR analysis of all genes potentially
involved in the secoiridoid biosynthetic pathway. After five days of MeJA treatment, an increase
of relative gene expression was detected for G8O, 8HGO7, IO, 7DLGT, 7DLH2, SLS and CPR1. On the
other hand, in centaury plants subjected to MeJA treatment for ten days, there was no significant
increase in gene expression. For GPPS and LAMT, decreased expression levels were detected, when compared to control plants. Patterns of SGs biosynthetic gene expression are highly correlated to
SGs’ profiles during MeJA-elicitation treatments, giving clear evidence that the identified genes
are a part of SGs’ biosynthetic routes. All the results indicate that MeJA is a suitable elicitor that
leads, through gene expression changes, to increased flux through the pathway and production
of swertiamarin, sweroside and gentiopicrin, which can be used for future production of secoiridoids
with the aid of biotechnological processes.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Joint Meeting of 3rd International Conference on Plant Biology, 22nd Symposium of the Serbian Plant Physiology Society. Belgrade, Serbia. Book of abstracts
T1  - Elicitation effects of methyl jasmonate on secoiridoid production in shoot culture of centaury (Centaurium erythraea Rafn)
SP  - 118
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4290
ER  - 

@conference{
author = "Lukić, Milana and Skorić, Marijana and Matekalo, Dragana and Božunović, Jelena and Aničić, Neda and Filipović, Biljana and Mišić, Danijela",
year = "2018",
abstract = "Centaurium erythraea (centaury), a plant with a long tradition of medicinal use, contains bioactive
secoiridoid glucosides (SGs). The exogenous application of methyl jasmonate (MeJA) may
elicit a response similar to stress, and is used in the present study to induce SG accumulation and
expression of SGs’ biosynthesis associated genes in centaury’s aerial parts. UHPLC-MS/MS analyses
have indicated that the addition of 250 μM MeJA to the medium has caused overproduction of
almost all investigated secondary metabolites. The most notable change was detected for swertiamarin
production, which was several fold-higher in MeJA-treated plants. For the purpose of determining
the key genes of MeJA response, we performed a qPCR analysis of all genes potentially
involved in the secoiridoid biosynthetic pathway. After five days of MeJA treatment, an increase
of relative gene expression was detected for G8O, 8HGO7, IO, 7DLGT, 7DLH2, SLS and CPR1. On the
other hand, in centaury plants subjected to MeJA treatment for ten days, there was no significant
increase in gene expression. For GPPS and LAMT, decreased expression levels were detected, when compared to control plants. Patterns of SGs biosynthetic gene expression are highly correlated to
SGs’ profiles during MeJA-elicitation treatments, giving clear evidence that the identified genes
are a part of SGs’ biosynthetic routes. All the results indicate that MeJA is a suitable elicitor that
leads, through gene expression changes, to increased flux through the pathway and production
of swertiamarin, sweroside and gentiopicrin, which can be used for future production of secoiridoids
with the aid of biotechnological processes.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Joint Meeting of 3rd International Conference on Plant Biology, 22nd Symposium of the Serbian Plant Physiology Society. Belgrade, Serbia. Book of abstracts",
title = "Elicitation effects of methyl jasmonate on secoiridoid production in shoot culture of centaury (Centaurium erythraea Rafn)",
pages = "118",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4290"
}

Lukić, M., Skorić, M., Matekalo, D., Božunović, J., Aničić, N., Filipović, B.,& Mišić, D.. (2018). Elicitation effects of methyl jasmonate on secoiridoid production in shoot culture of centaury (Centaurium erythraea Rafn). in Joint Meeting of 3rd International Conference on Plant Biology, 22nd Symposium of the Serbian Plant Physiology Society. Belgrade, Serbia. Book of abstracts
Belgrade: Serbian Plant Physiology Society., 118.
https://hdl.handle.net/21.15107/rcub_ibiss_4290

Lukić M, Skorić M, Matekalo D, Božunović J, Aničić N, Filipović B, Mišić D. Elicitation effects of methyl jasmonate on secoiridoid production in shoot culture of centaury (Centaurium erythraea Rafn). in Joint Meeting of 3rd International Conference on Plant Biology, 22nd Symposium of the Serbian Plant Physiology Society. Belgrade, Serbia. Book of abstracts. 2018;:118.
https://hdl.handle.net/21.15107/rcub_ibiss_4290 .

Lukić, Milana, Skorić, Marijana, Matekalo, Dragana, Božunović, Jelena, Aničić, Neda, Filipović, Biljana, Mišić, Danijela, "Elicitation effects of methyl jasmonate on secoiridoid production in shoot culture of centaury (Centaurium erythraea Rafn)" in Joint Meeting of 3rd International Conference on Plant Biology, 22nd Symposium of the Serbian Plant Physiology Society. Belgrade, Serbia. Book of abstracts (2018):118,
https://hdl.handle.net/21.15107/rcub_ibiss_4290 .