TY  - CONF
AU  - Mihailović, Vladimir
AU  - Katanić, Jelena
AU  - Mihailović, Mirjana
AU  - Mišić, Danijela
AU  - Solujić, Slavica
AU  - Šipovac, Katarina
AU  - Stanković, Vesna
AU  - Mladenović, Milan
AU  - Stanković, Nevena
PY  - 2013
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6143
AB  - Gentiana plants are best known for their bitter taste that is due to the secoiridoids (e.g. swertiamarin, gen­tiopicrin, sweroside and amarogentin). Secoiridoid glucosides, gentiopicrin, swertiamarin and sweroside, are present in various traditional medicine preparations and are reported to have hepatoprotective activity. Many Gentiana species are known for their pharmaceutical values, such as Gentiana cruciata L., commonly called cross gentian. The dried roots and above-ground parts of G. cruciata are consumed in the Balkan region as herbal tea or a medicinal wine for loss of appetite, as a stomachic and component in preparations showing benefi­cial effects in gall and liver diseases. This study using in vivo model investigates hepatoprotective activity of the methanol extract of G. cruciata root (GCR) against carbon tetrachloride-induced liver injury in rats. Wistar rats were orally pretreated with GCR (100, 200, and 400 mg/kg) and silymarin (100 mg/kg) for seven days be­fore they were treated with CCl4 (1 ml/kg, 1 :1 mixture in olive oil) which caused liver injury. Pretreatment with GCR dose-dependently and significantly (p < 0.001) decreased levels of serum transaminases, alkaline phos­phatase and total bilirubin, where as an increase was found in the level of total protein compared with CCl4-treated group. In the liver tissue antioxidant studies, we found a significant increase in the levels of catalase, superoxide dismutase and reduced glutathione, whereas there was marked reduction in the levels of thiobar­bituric acid-reactive substances, as compared to CC14 treated group. Histological analyses also show that GCR reduced the incidence of liver lesions including necrosis, ballooning degeneration and micro- and macrove­sicular changes induced by CC14 in rats. The main secoiridoid compounds (sweroside, swertiamarin and gen­tiopicrin) present in GCR were identified and quantified to gain an insight into the compounds responsible for its hepatoprotective effects. The HPLC assay clearly indicated that GCR contained the greatest concentra­tion of gentiopicrin (5.45%), whereas concentration of sweroside (0.29%) and swertiamarin (0.09%) were lower.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia
T1  - Secoiridoid content and hepatoprotective activity of Gentiana cruciata L. root extract
SP  - 91
EP  - 92
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6143
ER  - 

@conference{
author = "Mihailović, Vladimir and Katanić, Jelena and Mihailović, Mirjana and Mišić, Danijela and Solujić, Slavica and Šipovac, Katarina and Stanković, Vesna and Mladenović, Milan and Stanković, Nevena",
year = "2013",
abstract = "Gentiana plants are best known for their bitter taste that is due to the secoiridoids (e.g. swertiamarin, gen­tiopicrin, sweroside and amarogentin). Secoiridoid glucosides, gentiopicrin, swertiamarin and sweroside, are present in various traditional medicine preparations and are reported to have hepatoprotective activity. Many Gentiana species are known for their pharmaceutical values, such as Gentiana cruciata L., commonly called cross gentian. The dried roots and above-ground parts of G. cruciata are consumed in the Balkan region as herbal tea or a medicinal wine for loss of appetite, as a stomachic and component in preparations showing benefi­cial effects in gall and liver diseases. This study using in vivo model investigates hepatoprotective activity of the methanol extract of G. cruciata root (GCR) against carbon tetrachloride-induced liver injury in rats. Wistar rats were orally pretreated with GCR (100, 200, and 400 mg/kg) and silymarin (100 mg/kg) for seven days be­fore they were treated with CCl4 (1 ml/kg, 1 :1 mixture in olive oil) which caused liver injury. Pretreatment with GCR dose-dependently and significantly (p < 0.001) decreased levels of serum transaminases, alkaline phos­phatase and total bilirubin, where as an increase was found in the level of total protein compared with CCl4-treated group. In the liver tissue antioxidant studies, we found a significant increase in the levels of catalase, superoxide dismutase and reduced glutathione, whereas there was marked reduction in the levels of thiobar­bituric acid-reactive substances, as compared to CC14 treated group. Histological analyses also show that GCR reduced the incidence of liver lesions including necrosis, ballooning degeneration and micro- and macrove­sicular changes induced by CC14 in rats. The main secoiridoid compounds (sweroside, swertiamarin and gen­tiopicrin) present in GCR were identified and quantified to gain an insight into the compounds responsible for its hepatoprotective effects. The HPLC assay clearly indicated that GCR contained the greatest concentra­tion of gentiopicrin (5.45%), whereas concentration of sweroside (0.29%) and swertiamarin (0.09%) were lower.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia",
title = "Secoiridoid content and hepatoprotective activity of Gentiana cruciata L. root extract",
pages = "91-92",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6143"
}

Mihailović, V., Katanić, J., Mihailović, M., Mišić, D., Solujić, S., Šipovac, K., Stanković, V., Mladenović, M.,& Stanković, N.. (2013). Secoiridoid content and hepatoprotective activity of Gentiana cruciata L. root extract. in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia
Belgrade: Serbian Plant Physiology Society., 91-92.
https://hdl.handle.net/21.15107/rcub_ibiss_6143

Mihailović V, Katanić J, Mihailović M, Mišić D, Solujić S, Šipovac K, Stanković V, Mladenović M, Stanković N. Secoiridoid content and hepatoprotective activity of Gentiana cruciata L. root extract. in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia. 2013;:91-92.
https://hdl.handle.net/21.15107/rcub_ibiss_6143 .

Mihailović, Vladimir, Katanić, Jelena, Mihailović, Mirjana, Mišić, Danijela, Solujić, Slavica, Šipovac, Katarina, Stanković, Vesna, Mladenović, Milan, Stanković, Nevena, "Secoiridoid content and hepatoprotective activity of Gentiana cruciata L. root extract" in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia (2013):91-92,
https://hdl.handle.net/21.15107/rcub_ibiss_6143 .

TY  - JOUR
AU  - Mihailović, Vladimir
AU  - Mihailović, Mirjana
AU  - Uskoković, Aleksandra
AU  - Arambašić Jovanović, Jelena
AU  - Mišić, Danijela
AU  - Stanković, Vesna
AU  - Katanić, Jelena
AU  - Mladenović, Milan
AU  - Solujić, Slavica
AU  - Matić, Sanja
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1043
AB  - This study is an attempt to evaluate the hepatoprotective activity of Gentiana asclepiadea L against carbon tetrachloride-induced liver injury in rats. Methanol extracts of aerial parts (GM) and roots (GAR) of G. asclepiadea at doses of 100, 200, and 400 mg/kg b.w. were orally administered to Wistar rats once daily for 7 days before they were treated with CCl4. The hepatoprotective activity of the extracts in this study was compared with the reference drug silymarin. In CCl4 treated animals, GM and GAR significantly decreased levels of serum transaminases, alkaline phosphatase and total bilirubin, and increased the level of total protein. Treatment with the extracts resulted in a significant increase in the levels of catalase, superoxide dismutase and reduced glutathione, accompanied with a marked reduction in the levels of malondialdehyde, as compared to CCl4 treated group. The histopathological studies confirmed protective effects of extracts against CCl4-induced liver injuries. No genotoxicity was observed in liver cells after GM treatment, while GAR showed only slight genotoxic effects by comet assay. Phytochemical analysis revealed the presence of sweroside, swertiamarin and gentiopicrin in high concentrations in both extracts. It could be concluded that the use of G. asclepiadea extracts in the treatment of chemical-induced hepatotoxicity. (C) 2012 Elsevier Ltd. All rights reserved.
PB  - Elsevier
T2  - Food and Chemical Toxicology
T1  - Hepatoprotective effects of Gentiana asclepiadea L. extracts against carbon tetrachloride induced liver injury in rats
VL  - 52
DO  - 10.1016/j.fct.2012.10.034
SP  - 83
EP  - 90
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1043
ER  - 

@article{
author = "Mihailović, Vladimir and Mihailović, Mirjana and Uskoković, Aleksandra and Arambašić Jovanović, Jelena and Mišić, Danijela and Stanković, Vesna and Katanić, Jelena and Mladenović, Milan and Solujić, Slavica and Matić, Sanja",
year = "2013",
abstract = "This study is an attempt to evaluate the hepatoprotective activity of Gentiana asclepiadea L against carbon tetrachloride-induced liver injury in rats. Methanol extracts of aerial parts (GM) and roots (GAR) of G. asclepiadea at doses of 100, 200, and 400 mg/kg b.w. were orally administered to Wistar rats once daily for 7 days before they were treated with CCl4. The hepatoprotective activity of the extracts in this study was compared with the reference drug silymarin. In CCl4 treated animals, GM and GAR significantly decreased levels of serum transaminases, alkaline phosphatase and total bilirubin, and increased the level of total protein. Treatment with the extracts resulted in a significant increase in the levels of catalase, superoxide dismutase and reduced glutathione, accompanied with a marked reduction in the levels of malondialdehyde, as compared to CCl4 treated group. The histopathological studies confirmed protective effects of extracts against CCl4-induced liver injuries. No genotoxicity was observed in liver cells after GM treatment, while GAR showed only slight genotoxic effects by comet assay. Phytochemical analysis revealed the presence of sweroside, swertiamarin and gentiopicrin in high concentrations in both extracts. It could be concluded that the use of G. asclepiadea extracts in the treatment of chemical-induced hepatotoxicity. (C) 2012 Elsevier Ltd. All rights reserved.",
publisher = "Elsevier",
journal = "Food and Chemical Toxicology",
title = "Hepatoprotective effects of Gentiana asclepiadea L. extracts against carbon tetrachloride induced liver injury in rats",
volume = "52",
doi = "10.1016/j.fct.2012.10.034",
pages = "83-90",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1043"
}

Mihailović, V., Mihailović, M., Uskoković, A., Arambašić Jovanović, J., Mišić, D., Stanković, V., Katanić, J., Mladenović, M., Solujić, S.,& Matić, S.. (2013). Hepatoprotective effects of Gentiana asclepiadea L. extracts against carbon tetrachloride induced liver injury in rats. in Food and Chemical Toxicology
Elsevier., 52, 83-90.
https://doi.org/10.1016/j.fct.2012.10.034
https://hdl.handle.net/21.15107/rcub_ibiss_1043

Mihailović V, Mihailović M, Uskoković A, Arambašić Jovanović J, Mišić D, Stanković V, Katanić J, Mladenović M, Solujić S, Matić S. Hepatoprotective effects of Gentiana asclepiadea L. extracts against carbon tetrachloride induced liver injury in rats. in Food and Chemical Toxicology. 2013;52:83-90.
doi:10.1016/j.fct.2012.10.034
https://hdl.handle.net/21.15107/rcub_ibiss_1043 .

Mihailović, Vladimir, Mihailović, Mirjana, Uskoković, Aleksandra, Arambašić Jovanović, Jelena, Mišić, Danijela, Stanković, Vesna, Katanić, Jelena, Mladenović, Milan, Solujić, Slavica, Matić, Sanja, "Hepatoprotective effects of Gentiana asclepiadea L. extracts against carbon tetrachloride induced liver injury in rats" in Food and Chemical Toxicology, 52 (2013):83-90,
https://doi.org/10.1016/j.fct.2012.10.034 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1043 .

TY  - JOUR
AU  - Mihailović, Vladimir
AU  - Matić, Sanja
AU  - Mišić, Danijela
AU  - Solujić, Slavica R
AU  - Stanić, Snezana M
AU  - Katanić, Jelena
AU  - Mladenović, Milan P
AU  - Stanković, Nevena
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1049
AB  - The aim of this study was to evaluate the antioxidant and antigenotoxic activities of chloroform, ethyl acetate and n-butanol fractions obtained from Gentiana asclepiadea L. roots methanolic extract. The main secondary metabolites sweroside, swertiamarin and gentiopicrine were quantified in G. asclepiadea root extracts using HPLC-DAD analysis. Amount of total phenols, flavonoids, flavonols and gallotannins was also determined. The antigenotoxic potential of extracts from roots of G. asclepiadea was assessed using the standard in vivo procedure for the detection of sex linked recessive lethal mutations in Drosophila melanogaster males treated with ethyl methanesulfonate (EMS). The results showed that the most abundant secoiridoid in G. asclepiadea roots was gentiopicrine and its content in the n-butanol fraction (442.89 mg/g) was the highest. Among all extracts, ethyl acetate fraction showed the highest antioxidant activity, as well as total phenolics (146.64 GAE/g), flavonoids (44.62 RUE/g), flavonols (22.71 RUE/g) and gallotannins (0.99 mg GAE/g) content. All the fractions showed antioxidant activity using in vitro model systems and the results have been correlated with total phenolics, flavonoids, flavonols and gallotannins content. In addition to antioxidant activity, G. asclepiadea root extract fractions possess an antigenotoxic effect against DNA damage induced by alkylation with EMS. The antioxidant activity exhibited by G. asclepiadea depended on the phenolic compounds content of the tested extracts, while there was no significant difference in the antigenotoxic potential between fractions.
T2  - Excli Journal
T1  - Hemical Composition, Antioxidant and Antigenotoxic Activities of Different Fractions of Gentiana Asclepiadea L. Roots Extract
IS  - null
VL  - 12
SP  - 373
EP  - 823
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1049
ER  - 

@article{
author = "Mihailović, Vladimir and Matić, Sanja and Mišić, Danijela and Solujić, Slavica R and Stanić, Snezana M and Katanić, Jelena and Mladenović, Milan P and Stanković, Nevena",
year = "2013",
abstract = "The aim of this study was to evaluate the antioxidant and antigenotoxic activities of chloroform, ethyl acetate and n-butanol fractions obtained from Gentiana asclepiadea L. roots methanolic extract. The main secondary metabolites sweroside, swertiamarin and gentiopicrine were quantified in G. asclepiadea root extracts using HPLC-DAD analysis. Amount of total phenols, flavonoids, flavonols and gallotannins was also determined. The antigenotoxic potential of extracts from roots of G. asclepiadea was assessed using the standard in vivo procedure for the detection of sex linked recessive lethal mutations in Drosophila melanogaster males treated with ethyl methanesulfonate (EMS). The results showed that the most abundant secoiridoid in G. asclepiadea roots was gentiopicrine and its content in the n-butanol fraction (442.89 mg/g) was the highest. Among all extracts, ethyl acetate fraction showed the highest antioxidant activity, as well as total phenolics (146.64 GAE/g), flavonoids (44.62 RUE/g), flavonols (22.71 RUE/g) and gallotannins (0.99 mg GAE/g) content. All the fractions showed antioxidant activity using in vitro model systems and the results have been correlated with total phenolics, flavonoids, flavonols and gallotannins content. In addition to antioxidant activity, G. asclepiadea root extract fractions possess an antigenotoxic effect against DNA damage induced by alkylation with EMS. The antioxidant activity exhibited by G. asclepiadea depended on the phenolic compounds content of the tested extracts, while there was no significant difference in the antigenotoxic potential between fractions.",
journal = "Excli Journal",
title = "Hemical Composition, Antioxidant and Antigenotoxic Activities of Different Fractions of Gentiana Asclepiadea L. Roots Extract",
number = "null",
volume = "12",
pages = "373-823",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1049"
}

Mihailović, V., Matić, S., Mišić, D., Solujić, S. R., Stanić, S. M., Katanić, J., Mladenović, M. P.,& Stanković, N.. (2013). Hemical Composition, Antioxidant and Antigenotoxic Activities of Different Fractions of Gentiana Asclepiadea L. Roots Extract. in Excli Journal, 12(null), 373-823.
https://hdl.handle.net/21.15107/rcub_ibiss_1049

Mihailović V, Matić S, Mišić D, Solujić SR, Stanić SM, Katanić J, Mladenović MP, Stanković N. Hemical Composition, Antioxidant and Antigenotoxic Activities of Different Fractions of Gentiana Asclepiadea L. Roots Extract. in Excli Journal. 2013;12(null):373-823.
https://hdl.handle.net/21.15107/rcub_ibiss_1049 .

Mihailović, Vladimir, Matić, Sanja, Mišić, Danijela, Solujić, Slavica R, Stanić, Snezana M, Katanić, Jelena, Mladenović, Milan P, Stanković, Nevena, "Hemical Composition, Antioxidant and Antigenotoxic Activities of Different Fractions of Gentiana Asclepiadea L. Roots Extract" in Excli Journal, 12, no. null (2013):373-823,
https://hdl.handle.net/21.15107/rcub_ibiss_1049 .