TY  - JOUR
AU  - Berenyiova, Andrea
AU  - Grman, Marian
AU  - Mijuskovic, Ana
AU  - Stasko, Andrej
AU  - Misak, Anton
AU  - Nagy, Peter
AU  - Ondriasova, Elena
AU  - Cacanyiova, Sona
AU  - Brezova, Vlasta
AU  - Feelisch, Martin
AU  - Ondrias, Karol
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1969
AB  - The chemical interaction of sodium sulfide (Na2S) with the NO-donor
   S-nitrosoglutathione (GSNO) has been described to generate new reaction
   products, including polysulfides and nitrosopersulfide (SSNO-) via
   intermediacy of thionitrous acid (HSNO). The aim of the present work was
   to investigate the vascular effects of the longer-lived products of the
   Sulfide/GSNO interaction. Here we show that the products of this
   reaction relax precontracted isolated rings of rat thoracic aorta and
   mesenteric artery (but to a lesser degree rat uterus) with a >2-fold
   potency compared with the starting material, GSNO (50 nM), whereas Na2S
   and polysulfides have little effect at 1-5 mu M. The onset of
   vasorelaxation of the reaction products was 7-10 times faster in aorta
   and mesenteric arteries compared with GSNO. Relaxation to GSNO (100-500
   nM) was blocked by an inhibitor of soluble guanylyl cyclase, ODQ (0.1
   and 10 mu M), and by the NO scavenger cPTIO (100 mu M), but less
   affected by prior acidification (pH 2-4), and unaffected by
   N-acetylcysteine (1 mM) or methemoglobin (20 mu M heme). By contrast,
   relaxation to the Sulfide/GSNO reaction products (100-500 nM based on
   the starting material) was inhibited to a lesser extent by ODQ only
   slightly decreased by cPTIO, more markedly inhibited by methemoglobin
   and N-acetylcysteine, and abolished by acidification before addition to
   the organ bath. The reaction mixture was found to generate NO as
   detected by EPR spectroscopy using
   N-(dithiocarboxy)-N-methyl-D-glucamine (MGD(2))-Fe2+ as spin trap. In
   conclusion, the Sufide/GSNO reaction products are faster and more
   pronounced vasorelaxants than GSNO itself. We conclude that in addition
   to NO formation from SSNO-, reaction products other than polysulfides
   may give rise to nitroxyl (HNO) and be involved in the pronounced
   relaxation induced by the Sulfide/GSNO cross-talk. (C) 2014 Elsevier
   Inc. All rights reserved.
T2  - Nitric Oxide-Biology and Chemistry
T1  - The reaction products of sulfide and S-nitrosoglutathione are potent
 vasorelaxants
IS  - SI
VL  - 46
DO  - 10.1016/j.niox.2014.12.008
SP  - 123
EP  - 130
ER  - 

@article{
author = "Berenyiova, Andrea and Grman, Marian and Mijuskovic, Ana and Stasko, Andrej and Misak, Anton and Nagy, Peter and Ondriasova, Elena and Cacanyiova, Sona and Brezova, Vlasta and Feelisch, Martin and Ondrias, Karol",
year = "2015",
abstract = "The chemical interaction of sodium sulfide (Na2S) with the NO-donor
   S-nitrosoglutathione (GSNO) has been described to generate new reaction
   products, including polysulfides and nitrosopersulfide (SSNO-) via
   intermediacy of thionitrous acid (HSNO). The aim of the present work was
   to investigate the vascular effects of the longer-lived products of the
   Sulfide/GSNO interaction. Here we show that the products of this
   reaction relax precontracted isolated rings of rat thoracic aorta and
   mesenteric artery (but to a lesser degree rat uterus) with a >2-fold
   potency compared with the starting material, GSNO (50 nM), whereas Na2S
   and polysulfides have little effect at 1-5 mu M. The onset of
   vasorelaxation of the reaction products was 7-10 times faster in aorta
   and mesenteric arteries compared with GSNO. Relaxation to GSNO (100-500
   nM) was blocked by an inhibitor of soluble guanylyl cyclase, ODQ (0.1
   and 10 mu M), and by the NO scavenger cPTIO (100 mu M), but less
   affected by prior acidification (pH 2-4), and unaffected by
   N-acetylcysteine (1 mM) or methemoglobin (20 mu M heme). By contrast,
   relaxation to the Sulfide/GSNO reaction products (100-500 nM based on
   the starting material) was inhibited to a lesser extent by ODQ only
   slightly decreased by cPTIO, more markedly inhibited by methemoglobin
   and N-acetylcysteine, and abolished by acidification before addition to
   the organ bath. The reaction mixture was found to generate NO as
   detected by EPR spectroscopy using
   N-(dithiocarboxy)-N-methyl-D-glucamine (MGD(2))-Fe2+ as spin trap. In
   conclusion, the Sufide/GSNO reaction products are faster and more
   pronounced vasorelaxants than GSNO itself. We conclude that in addition
   to NO formation from SSNO-, reaction products other than polysulfides
   may give rise to nitroxyl (HNO) and be involved in the pronounced
   relaxation induced by the Sulfide/GSNO cross-talk. (C) 2014 Elsevier
   Inc. All rights reserved.",
journal = "Nitric Oxide-Biology and Chemistry",
title = "The reaction products of sulfide and S-nitrosoglutathione are potent
 vasorelaxants",
number = "SI",
volume = "46",
doi = "10.1016/j.niox.2014.12.008",
pages = "123-130"
}

Berenyiova, A., Grman, M., Mijuskovic, A., Stasko, A., Misak, A., Nagy, P., Ondriasova, E., Cacanyiova, S., Brezova, V., Feelisch, M.,& Ondrias, K.. (2015). The reaction products of sulfide and S-nitrosoglutathione are potent
 vasorelaxants. in Nitric Oxide-Biology and Chemistry, 46(SI), 123-130.
https://doi.org/10.1016/j.niox.2014.12.008

Berenyiova A, Grman M, Mijuskovic A, Stasko A, Misak A, Nagy P, Ondriasova E, Cacanyiova S, Brezova V, Feelisch M, Ondrias K. The reaction products of sulfide and S-nitrosoglutathione are potent
 vasorelaxants. in Nitric Oxide-Biology and Chemistry. 2015;46(SI):123-130.
doi:10.1016/j.niox.2014.12.008 .

Berenyiova, Andrea, Grman, Marian, Mijuskovic, Ana, Stasko, Andrej, Misak, Anton, Nagy, Peter, Ondriasova, Elena, Cacanyiova, Sona, Brezova, Vlasta, Feelisch, Martin, Ondrias, Karol, "The reaction products of sulfide and S-nitrosoglutathione are potent
 vasorelaxants" in Nitric Oxide-Biology and Chemistry, 46, no. SI (2015):123-130,
https://doi.org/10.1016/j.niox.2014.12.008 . .