TY  - CONF
AU  - Rajić, Jovana
AU  - Grdović, Nevena
AU  - Inić-Kanada, Aleksandra
AU  - Stein, Elisabath
AU  - Schuerer, Nadine
AU  - Uskoković, Aleksandra
AU  - Ghasemian, Ehsan
AU  - Dinić, Svetlana
AU  - Mihailović, Mirjana
AU  - Arambašić Jovanović, Jelena
AU  - Tolić, Anja
AU  - Đorđević, Marija
AU  - Đorđević, Miloš
AU  - Poznanović, Goran
AU  - Barisani-Asenbauer, Talin
AU  - Vidaković, Melita
PY  - 2017
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5814
AB  - Repeated eye infections with Chlamydia trachomatis (Ct) usually lead to conjunctiva! scarring, corneal damage and blindness (trachoma). Considering epithelial to mesenchymal transition (EMD as unavoidable player in the development of fibrosis and taking into account that EMT is epigenetically reg­ulated process, our intention was to explore whether Ct infec­tion induces EMT in vitro and to uncover potential underlying epigenetic mechanisms. 
Human conjunctiva! epithelial cells were infected with 107 IFU of Ct for 72 h. The mRNA and protein expression of EMT mark­ers (E-cadherin and fibronectin) were assessed by RT-qPCR, lmmunoblotting and lmmunocytochemfstry. DNA methyfation analysis of selected regions of marker genes were examined by MSP, HRM and Bs-Seq. 
The Ct infection induced EMT-related changes in mRNA and protein expression, downregulation of epithelial marker E-cadherin and upregulation of mesenchymal marker fibronectin. Increase in DNA methylation of E-cadherin gene promoter was in correlation with its decreased expression, while DNA meth­ylation status of fibronectin gene couldn't be related to its ex­pression level. 
We suggest that manipulating the EMT process via modula­tion of DNA methylation of E-cadherin opens up possibilities to stop or revers the progression of scarring as a new strategy in trachoma treatment.
PB  - Institute of Biology and Immunology of Reproduction, BAS, COST Action CA16119 and Mouseprint Ltd.
C3  - Proceedings of CellFit meeting: In vitro 3-D total cell guidance and fitness; 2017 Sep 12-14; Albena Resort, Bulgaria
T1  - Chlamydia trachomatis infection triggers  Epithelial Mesenchymal Transition in  conjunctiva: involvement of DNA methylation
SP  - 64
EP  - 65
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5814
ER  - 

@conference{
author = "Rajić, Jovana and Grdović, Nevena and Inić-Kanada, Aleksandra and Stein, Elisabath and Schuerer, Nadine and Uskoković, Aleksandra and Ghasemian, Ehsan and Dinić, Svetlana and Mihailović, Mirjana and Arambašić Jovanović, Jelena and Tolić, Anja and Đorđević, Marija and Đorđević, Miloš and Poznanović, Goran and Barisani-Asenbauer, Talin and Vidaković, Melita",
year = "2017",
abstract = "Repeated eye infections with Chlamydia trachomatis (Ct) usually lead to conjunctiva! scarring, corneal damage and blindness (trachoma). Considering epithelial to mesenchymal transition (EMD as unavoidable player in the development of fibrosis and taking into account that EMT is epigenetically reg­ulated process, our intention was to explore whether Ct infec­tion induces EMT in vitro and to uncover potential underlying epigenetic mechanisms. 
Human conjunctiva! epithelial cells were infected with 107 IFU of Ct for 72 h. The mRNA and protein expression of EMT mark­ers (E-cadherin and fibronectin) were assessed by RT-qPCR, lmmunoblotting and lmmunocytochemfstry. DNA methyfation analysis of selected regions of marker genes were examined by MSP, HRM and Bs-Seq. 
The Ct infection induced EMT-related changes in mRNA and protein expression, downregulation of epithelial marker E-cadherin and upregulation of mesenchymal marker fibronectin. Increase in DNA methylation of E-cadherin gene promoter was in correlation with its decreased expression, while DNA meth­ylation status of fibronectin gene couldn't be related to its ex­pression level. 
We suggest that manipulating the EMT process via modula­tion of DNA methylation of E-cadherin opens up possibilities to stop or revers the progression of scarring as a new strategy in trachoma treatment.",
publisher = "Institute of Biology and Immunology of Reproduction, BAS, COST Action CA16119 and Mouseprint Ltd.",
journal = "Proceedings of CellFit meeting: In vitro 3-D total cell guidance and fitness; 2017 Sep 12-14; Albena Resort, Bulgaria",
title = "Chlamydia trachomatis infection triggers  Epithelial Mesenchymal Transition in  conjunctiva: involvement of DNA methylation",
pages = "64-65",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5814"
}

Rajić, J., Grdović, N., Inić-Kanada, A., Stein, E., Schuerer, N., Uskoković, A., Ghasemian, E., Dinić, S., Mihailović, M., Arambašić Jovanović, J., Tolić, A., Đorđević, M., Đorđević, M., Poznanović, G., Barisani-Asenbauer, T.,& Vidaković, M.. (2017). Chlamydia trachomatis infection triggers  Epithelial Mesenchymal Transition in  conjunctiva: involvement of DNA methylation. in Proceedings of CellFit meeting: In vitro 3-D total cell guidance and fitness; 2017 Sep 12-14; Albena Resort, Bulgaria
Institute of Biology and Immunology of Reproduction, BAS, COST Action CA16119 and Mouseprint Ltd.., 64-65.
https://hdl.handle.net/21.15107/rcub_ibiss_5814

Rajić J, Grdović N, Inić-Kanada A, Stein E, Schuerer N, Uskoković A, Ghasemian E, Dinić S, Mihailović M, Arambašić Jovanović J, Tolić A, Đorđević M, Đorđević M, Poznanović G, Barisani-Asenbauer T, Vidaković M. Chlamydia trachomatis infection triggers  Epithelial Mesenchymal Transition in  conjunctiva: involvement of DNA methylation. in Proceedings of CellFit meeting: In vitro 3-D total cell guidance and fitness; 2017 Sep 12-14; Albena Resort, Bulgaria. 2017;:64-65.
https://hdl.handle.net/21.15107/rcub_ibiss_5814 .

Rajić, Jovana, Grdović, Nevena, Inić-Kanada, Aleksandra, Stein, Elisabath, Schuerer, Nadine, Uskoković, Aleksandra, Ghasemian, Ehsan, Dinić, Svetlana, Mihailović, Mirjana, Arambašić Jovanović, Jelena, Tolić, Anja, Đorđević, Marija, Đorđević, Miloš, Poznanović, Goran, Barisani-Asenbauer, Talin, Vidaković, Melita, "Chlamydia trachomatis infection triggers  Epithelial Mesenchymal Transition in  conjunctiva: involvement of DNA methylation" in Proceedings of CellFit meeting: In vitro 3-D total cell guidance and fitness; 2017 Sep 12-14; Albena Resort, Bulgaria (2017):64-65,
https://hdl.handle.net/21.15107/rcub_ibiss_5814 .