TY  - JOUR
AU  - Dmitrović, Slavica
AU  - Banjac, Nevena
AU  - Zdravković-Korać, Snežana
AU  - Vinterhalter, Branka
AU  - Ninković, Slavica
AU  - Ćulafić, Ljubinka
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1345
AB  - Susceptibility of C. rubrum to Agrobacterium-mediated transformation was demonstrated by inoculating the petioles of in vitro grown plants with A. rhizogenes strain A4M70GUS. Hairy roots were produced in 8 % of explants. They were isolated and maintained on plant growth regulator-free solid or liquid half-strength Murashige and Skoog medium for two years. Hairy root fresh mass increased 30 - 90 folds when grown in liquid medium, which was superior to solid medium, where most of the hairy roots produced calli. When these calli were grown on medium supplemented with 0.5 mg dm-3 thidiazuron, embryo-like structures were obtained. Transgenic status of long-term callus and hairy root cultures was confirmed by histochemical GUS assay, by PCR specific to the uidA, rolA&B and ags genes and by Southern hybridization.
T2  - Biologia Plantarum
T1  - Hairy roots formation in recalcitrant-to-transform plant Chenopodium rubrum
IS  - 3
VL  - 54
EP  - 570
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1345
ER  - 

@article{
author = "Dmitrović, Slavica and Banjac, Nevena and Zdravković-Korać, Snežana and Vinterhalter, Branka and Ninković, Slavica and Ćulafić, Ljubinka",
year = "2010",
abstract = "Susceptibility of C. rubrum to Agrobacterium-mediated transformation was demonstrated by inoculating the petioles of in vitro grown plants with A. rhizogenes strain A4M70GUS. Hairy roots were produced in 8 % of explants. They were isolated and maintained on plant growth regulator-free solid or liquid half-strength Murashige and Skoog medium for two years. Hairy root fresh mass increased 30 - 90 folds when grown in liquid medium, which was superior to solid medium, where most of the hairy roots produced calli. When these calli were grown on medium supplemented with 0.5 mg dm-3 thidiazuron, embryo-like structures were obtained. Transgenic status of long-term callus and hairy root cultures was confirmed by histochemical GUS assay, by PCR specific to the uidA, rolA&B and ags genes and by Southern hybridization.",
journal = "Biologia Plantarum",
title = "Hairy roots formation in recalcitrant-to-transform plant Chenopodium rubrum",
number = "3",
volume = "54",
pages = "570",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1345"
}

Dmitrović, S., Banjac, N., Zdravković-Korać, S., Vinterhalter, B., Ninković, S.,& Ćulafić, L.. (2010). Hairy roots formation in recalcitrant-to-transform plant Chenopodium rubrum. in Biologia Plantarum, 54(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1345

Dmitrović S, Banjac N, Zdravković-Korać S, Vinterhalter B, Ninković S, Ćulafić L. Hairy roots formation in recalcitrant-to-transform plant Chenopodium rubrum. in Biologia Plantarum. 2010;54(3):null-570.
https://hdl.handle.net/21.15107/rcub_ibiss_1345 .

Dmitrović, Slavica, Banjac, Nevena, Zdravković-Korać, Snežana, Vinterhalter, Branka, Ninković, Slavica, Ćulafić, Ljubinka, "Hairy roots formation in recalcitrant-to-transform plant Chenopodium rubrum" in Biologia Plantarum, 54, no. 3 (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1345 .

TY  - JOUR
AU  - Stojičić, Dragana
AU  - Uzelac, Branka
AU  - Janošević, Dušica
AU  - Ćulafić, Ljubinka
AU  - Budimir, Snežana
PY  - 2007
PY  - 2007
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/159
AB  - The potential for somatic embryogenesis in zygotic embryo and megagametophyte cultures of Pinus heldreichii was examined. Somatic embryogenesis was initiated from megagametophytes containing immature zygotic embryos at early stages of development. An induction frequency of up to 6.7% was obtained on Gresshoff and Doy medium in the presence of 2 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5 mg/l benzyladenine (BA). Formation and further proliferation of embryogenic tissue were achieved upon transfer of explants to a medium with reduced levels of growth regulators. Somatic embryos are being cultured for further development. .
AB  - U kulturi izolovanih zigotskih embriona i ovula munike (Pinus heldreichii) ispitivan je efekat hranljive podloge i regulatora rastenja na indukciju somatske embriogeneze. Somatska embriogeneza je indukovana u kulturi ovula koje su sadržavale embrione na ranom stupnju razvića. Najviša frekvencija indukcije od 6.7% postignuta je kada su ovule 5 dana gajene na Gresshoff i Doy (GD) hranljivoj podlozi u prisustvu 2,4-D 2 mg/l i BA 0.5 mg/l, a zatim prenete na GD podlogu u kojoj je koncentracija regulatora rastenja bila 5 puta niža. U cilju dugotrajnog održavanja u kulturi, embriogeno tkivo je izolovano i gajeno na GD podlozi sa 2,4-D 0.2 mg/l i BA 0.05 mg/l. Mikroskopskom analizom je utvrđeno da se tkivo sastoji od brojnih embriona na ranim stupnjevima razvića. Za klonalnu propagaciju ove značajne endemoreliktne četinarske vrste u kulturi in vitro, neophodna su dalja istraživanja sa ciljem povećanja frekvencije indukcije somatske embriogeneze, kao i frekvencije sazrevanja embriona. .
T2  - Archives of Biological Sciences
T1  - Indukcija somatske embriogeneze kod Pinus heldreichii u kulturi
T1  - Induction of somatic embryogenesis in Pinus heldreichii culture
IS  - 3
VL  - 59
SP  - 199
EP  - 202
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_159
ER  - 

@article{
author = "Stojičić, Dragana and Uzelac, Branka and Janošević, Dušica and Ćulafić, Ljubinka and Budimir, Snežana",
year = "2007, 2007",
abstract = "The potential for somatic embryogenesis in zygotic embryo and megagametophyte cultures of Pinus heldreichii was examined. Somatic embryogenesis was initiated from megagametophytes containing immature zygotic embryos at early stages of development. An induction frequency of up to 6.7% was obtained on Gresshoff and Doy medium in the presence of 2 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5 mg/l benzyladenine (BA). Formation and further proliferation of embryogenic tissue were achieved upon transfer of explants to a medium with reduced levels of growth regulators. Somatic embryos are being cultured for further development. ., U kulturi izolovanih zigotskih embriona i ovula munike (Pinus heldreichii) ispitivan je efekat hranljive podloge i regulatora rastenja na indukciju somatske embriogeneze. Somatska embriogeneza je indukovana u kulturi ovula koje su sadržavale embrione na ranom stupnju razvića. Najviša frekvencija indukcije od 6.7% postignuta je kada su ovule 5 dana gajene na Gresshoff i Doy (GD) hranljivoj podlozi u prisustvu 2,4-D 2 mg/l i BA 0.5 mg/l, a zatim prenete na GD podlogu u kojoj je koncentracija regulatora rastenja bila 5 puta niža. U cilju dugotrajnog održavanja u kulturi, embriogeno tkivo je izolovano i gajeno na GD podlozi sa 2,4-D 0.2 mg/l i BA 0.05 mg/l. Mikroskopskom analizom je utvrđeno da se tkivo sastoji od brojnih embriona na ranim stupnjevima razvića. Za klonalnu propagaciju ove značajne endemoreliktne četinarske vrste u kulturi in vitro, neophodna su dalja istraživanja sa ciljem povećanja frekvencije indukcije somatske embriogeneze, kao i frekvencije sazrevanja embriona. .",
journal = "Archives of Biological Sciences",
title = "Indukcija somatske embriogeneze kod Pinus heldreichii u kulturi, Induction of somatic embryogenesis in Pinus heldreichii culture",
number = "3",
volume = "59",
pages = "199-202",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_159"
}

Stojičić, D., Uzelac, B., Janošević, D., Ćulafić, L.,& Budimir, S.. (2007). Indukcija somatske embriogeneze kod Pinus heldreichii u kulturi. in Archives of Biological Sciences, 59(3), 199-202.
https://hdl.handle.net/21.15107/rcub_ibiss_159

Stojičić D, Uzelac B, Janošević D, Ćulafić L, Budimir S. Indukcija somatske embriogeneze kod Pinus heldreichii u kulturi. in Archives of Biological Sciences. 2007;59(3):199-202.
https://hdl.handle.net/21.15107/rcub_ibiss_159 .

Stojičić, Dragana, Uzelac, Branka, Janošević, Dušica, Ćulafić, Ljubinka, Budimir, Snežana, "Indukcija somatske embriogeneze kod Pinus heldreichii u kulturi" in Archives of Biological Sciences, 59, no. 3 (2007):199-202,
https://hdl.handle.net/21.15107/rcub_ibiss_159 .

TY  - JOUR
AU  - Dmitrović, Slavica
AU  - Mitrović, Aleksandra
AU  - Ćulafić, Ljubinka
PY  - 2005
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1704
AB  - In order to establish an efficient system for in vitro plant regeneration of a short day plant Chenopodium rubrum L. and a long day plant Chenopodium murale L., optimum culture conditions for somatic embryogenesis were investigated. The effects of different growth regulators, their combination and their concentrations on somatic embryos induction in different explant types (root, hypocotyl, cotyledon and leaf) were tested. Somatic embryogenesis was induced in both plants on Murashige and Skoog (MS) medium supplemented with sucrose (3%), agar (0.7%) and 1-10 mu M 2,4-dichlorophenoxyacetic acid (2,4-D) as the sole growth regulator. The largest embryogenic capacity was found in root explants of Chenopodium rubrum on 1 mu M 2,4-D and in basal parts of cotyledons in C. murale plants on 10 mu M 2,4-D.
T2  - Biologia Plantarum
T1  - Somatic embryogenesis in Chenopodium rubrum and Chenopodium murale in vitro
IS  - 1
VL  - 49
DO  - 10.1007/s00000-005-5039-5
SP  - 35
EP  - 39
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1704
ER  - 

@article{
author = "Dmitrović, Slavica and Mitrović, Aleksandra and Ćulafić, Ljubinka",
year = "2005",
abstract = "In order to establish an efficient system for in vitro plant regeneration of a short day plant Chenopodium rubrum L. and a long day plant Chenopodium murale L., optimum culture conditions for somatic embryogenesis were investigated. The effects of different growth regulators, their combination and their concentrations on somatic embryos induction in different explant types (root, hypocotyl, cotyledon and leaf) were tested. Somatic embryogenesis was induced in both plants on Murashige and Skoog (MS) medium supplemented with sucrose (3%), agar (0.7%) and 1-10 mu M 2,4-dichlorophenoxyacetic acid (2,4-D) as the sole growth regulator. The largest embryogenic capacity was found in root explants of Chenopodium rubrum on 1 mu M 2,4-D and in basal parts of cotyledons in C. murale plants on 10 mu M 2,4-D.",
journal = "Biologia Plantarum",
title = "Somatic embryogenesis in Chenopodium rubrum and Chenopodium murale in vitro",
number = "1",
volume = "49",
doi = "10.1007/s00000-005-5039-5",
pages = "35-39",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1704"
}

Dmitrović, S., Mitrović, A.,& Ćulafić, L.. (2005). Somatic embryogenesis in Chenopodium rubrum and Chenopodium murale in vitro. in Biologia Plantarum, 49(1), 35-39.
https://doi.org/10.1007/s00000-005-5039-5
https://hdl.handle.net/21.15107/rcub_ibiss_1704

Dmitrović S, Mitrović A, Ćulafić L. Somatic embryogenesis in Chenopodium rubrum and Chenopodium murale in vitro. in Biologia Plantarum. 2005;49(1):35-39.
doi:10.1007/s00000-005-5039-5
https://hdl.handle.net/21.15107/rcub_ibiss_1704 .

Dmitrović, Slavica, Mitrović, Aleksandra, Ćulafić, Ljubinka, "Somatic embryogenesis in Chenopodium rubrum and Chenopodium murale in vitro" in Biologia Plantarum, 49, no. 1 (2005):35-39,
https://doi.org/10.1007/s00000-005-5039-5 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1704 .

TY  - JOUR
AU  - Mitrović, Aleksandra
AU  - Vinterhalter, Branka
AU  - Ćulafić, Ljubinka
PY  - 2005
PY  - 2005
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/358
AB  - Tacitus bellus Moran & J. Meyran, syn. Graptopetalum bellum, fam Crassulaceae was discovered in 1972 in a mountainous areas in Mexico and southwest of the USA. Rosettes, up to 10 cm in diameter, lie almost flat to the ground, while large clusters of star like, pink till deep red, flowers appear in May and June, which makes it one of the most fascinating new succulent plants. Sterilized lower rosette leaves were aseptically placed on medium containing, mineral and vitamin solution supplemented with sucrose (3 %), benzylaminopurine (BAP) (2.25 mg dm-3) and naphtalenacetic acid (NAA) (0.186 mg dm-3) arid gelled with agar (0.7 %), Murashige and Skoog, 1962,. Callus and a large number of buds were observed on the explants bases after six months of cultivation on the two-phase medium. The bud multiplications were performed on MS media supplemented with BAP (0.1-0.5 mg dm-3) and NAA (0.1 mg dm-3), while rooting of small rosettes were performed on NAA (0.1 - 0.2 mg dm-3). Spontaneous rooting (80 %) were noticed on hormone free medium while NAA (0.1 or 0.2 mg dm-3) stimulated it up to 100%, depending on clones. Rooted plants were easily acclimatized.
AB  - Tacitus bellus Moran & J. Meyran, syn. Graptopetalum bellum, fam Crassulaceae je pronađena 1972 godine u planinskim regionima Meksika i jugozapadne Amerike. Rozete, 10 cm u prečniku, leže gotovo na zemlji. Stabljike sa mnogobrojnim zvezdolikim ružičastim cvetovima, pojavljuju u maju i junu, što ovu biljku čini jednom od najprivlačnijih sukulentnih biljaka. Površinski sterilisani donji listovi rozete su postavljani na podlogu koja sadrži MS mineralni i vitaminski rastvor, saharozu (3 %), BAP (2.25 mg dm-3) i NAA (0.186 mg dm-3) i agar (0.7 %). Posle šest meseci kultivacije na dvofaznom medijumu, primećen je kalus i veliki broj pupoljaka u osnovi eksplantata. Medijumi za multiplikaciju pupoljaka sadržali su BAP (0.1 - 0.5 mg dm-3) i NAA (0.1 mg dm-3), dok su medijumi za ožiljavanje malih rozta sadržali NAA (0.1 ili 0.2 mg dm-3). Spontano ožiljavanje (80 %) je uočeno na podlozi bez hormona, dok je NAA (0.1 i 0.2 mg dm-3) stimulisala ožiljavanje do 100%, u zavisnosti od klona. Ožiljene biljke su lako aklimatizovane.
T2  - Journal of Scientific Agricultural Research
T1  - In vitro propagacija Tacitus bellus
T1  - In vitro propagation of Tacitus belhts
IS  - 1
VL  - 66
SP  - 33
EP  - 39
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_358
ER  - 

@article{
author = "Mitrović, Aleksandra and Vinterhalter, Branka and Ćulafić, Ljubinka",
year = "2005, 2005",
abstract = "Tacitus bellus Moran & J. Meyran, syn. Graptopetalum bellum, fam Crassulaceae was discovered in 1972 in a mountainous areas in Mexico and southwest of the USA. Rosettes, up to 10 cm in diameter, lie almost flat to the ground, while large clusters of star like, pink till deep red, flowers appear in May and June, which makes it one of the most fascinating new succulent plants. Sterilized lower rosette leaves were aseptically placed on medium containing, mineral and vitamin solution supplemented with sucrose (3 %), benzylaminopurine (BAP) (2.25 mg dm-3) and naphtalenacetic acid (NAA) (0.186 mg dm-3) arid gelled with agar (0.7 %), Murashige and Skoog, 1962,. Callus and a large number of buds were observed on the explants bases after six months of cultivation on the two-phase medium. The bud multiplications were performed on MS media supplemented with BAP (0.1-0.5 mg dm-3) and NAA (0.1 mg dm-3), while rooting of small rosettes were performed on NAA (0.1 - 0.2 mg dm-3). Spontaneous rooting (80 %) were noticed on hormone free medium while NAA (0.1 or 0.2 mg dm-3) stimulated it up to 100%, depending on clones. Rooted plants were easily acclimatized., Tacitus bellus Moran & J. Meyran, syn. Graptopetalum bellum, fam Crassulaceae je pronađena 1972 godine u planinskim regionima Meksika i jugozapadne Amerike. Rozete, 10 cm u prečniku, leže gotovo na zemlji. Stabljike sa mnogobrojnim zvezdolikim ružičastim cvetovima, pojavljuju u maju i junu, što ovu biljku čini jednom od najprivlačnijih sukulentnih biljaka. Površinski sterilisani donji listovi rozete su postavljani na podlogu koja sadrži MS mineralni i vitaminski rastvor, saharozu (3 %), BAP (2.25 mg dm-3) i NAA (0.186 mg dm-3) i agar (0.7 %). Posle šest meseci kultivacije na dvofaznom medijumu, primećen je kalus i veliki broj pupoljaka u osnovi eksplantata. Medijumi za multiplikaciju pupoljaka sadržali su BAP (0.1 - 0.5 mg dm-3) i NAA (0.1 mg dm-3), dok su medijumi za ožiljavanje malih rozta sadržali NAA (0.1 ili 0.2 mg dm-3). Spontano ožiljavanje (80 %) je uočeno na podlozi bez hormona, dok je NAA (0.1 i 0.2 mg dm-3) stimulisala ožiljavanje do 100%, u zavisnosti od klona. Ožiljene biljke su lako aklimatizovane.",
journal = "Journal of Scientific Agricultural Research",
title = "In vitro propagacija Tacitus bellus, In vitro propagation of Tacitus belhts",
number = "1",
volume = "66",
pages = "33-39",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_358"
}

Mitrović, A., Vinterhalter, B.,& Ćulafić, L.. (2005). In vitro propagacija Tacitus bellus. in Journal of Scientific Agricultural Research, 66(1), 33-39.
https://hdl.handle.net/21.15107/rcub_ibiss_358

Mitrović A, Vinterhalter B, Ćulafić L. In vitro propagacija Tacitus bellus. in Journal of Scientific Agricultural Research. 2005;66(1):33-39.
https://hdl.handle.net/21.15107/rcub_ibiss_358 .

Mitrović, Aleksandra, Vinterhalter, Branka, Ćulafić, Ljubinka, "In vitro propagacija Tacitus bellus" in Journal of Scientific Agricultural Research, 66, no. 1 (2005):33-39,
https://hdl.handle.net/21.15107/rcub_ibiss_358 .

TY  - JOUR
AU  - Bavrina, TV
AU  - Lozhnikova, VN
AU  - Ćulafić, Ljubinka
AU  - Živanović, Branka D
PY  - 2002
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1793
AB  - Green plants and plants devoid of photosynthetic pigments were compared with regard to their ability to flower under various growth conditions. Green plants of Chenopodium rubrum L. and plants treated with norflurazon SANDOZ-9789 (SAN) were grown on sucrose-containing media with or without hormones (GA(3), BA, IAA, ABA) under short-day photoperiodic or continuous illumination with white, blue, or red light. Green and SAN-treated albino plants produced flowers only under short-day conditions. The flowering of green plants was independent of the presence of sucrose and hormones in the medium as well as of the light quality. The albino plants produced flowers under white and blue light but did not flower in red light. The addition of GA(3) or BA to the medium induced flowering of albino plants exposed to red light. The functional interaction of photoreceptors in the flowering control is discussed.
T2  - Russian Journal of Plant Physiology
T1  - Flowering of cultivated green and SAN 9789-treated Chenopodium rubrum plants exposed to white, blue, and red light
IS  - 4
VL  - 49
EP  - 464
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1793
ER  - 

@article{
author = "Bavrina, TV and Lozhnikova, VN and Ćulafić, Ljubinka and Živanović, Branka D",
year = "2002",
abstract = "Green plants and plants devoid of photosynthetic pigments were compared with regard to their ability to flower under various growth conditions. Green plants of Chenopodium rubrum L. and plants treated with norflurazon SANDOZ-9789 (SAN) were grown on sucrose-containing media with or without hormones (GA(3), BA, IAA, ABA) under short-day photoperiodic or continuous illumination with white, blue, or red light. Green and SAN-treated albino plants produced flowers only under short-day conditions. The flowering of green plants was independent of the presence of sucrose and hormones in the medium as well as of the light quality. The albino plants produced flowers under white and blue light but did not flower in red light. The addition of GA(3) or BA to the medium induced flowering of albino plants exposed to red light. The functional interaction of photoreceptors in the flowering control is discussed.",
journal = "Russian Journal of Plant Physiology",
title = "Flowering of cultivated green and SAN 9789-treated Chenopodium rubrum plants exposed to white, blue, and red light",
number = "4",
volume = "49",
pages = "464",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1793"
}

Bavrina, T., Lozhnikova, V., Ćulafić, L.,& Živanović, B. D.. (2002). Flowering of cultivated green and SAN 9789-treated Chenopodium rubrum plants exposed to white, blue, and red light. in Russian Journal of Plant Physiology, 49(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1793

Bavrina T, Lozhnikova V, Ćulafić L, Živanović BD. Flowering of cultivated green and SAN 9789-treated Chenopodium rubrum plants exposed to white, blue, and red light. in Russian Journal of Plant Physiology. 2002;49(4):null-464.
https://hdl.handle.net/21.15107/rcub_ibiss_1793 .

Bavrina, TV, Lozhnikova, VN, Ćulafić, Ljubinka, Živanović, Branka D, "Flowering of cultivated green and SAN 9789-treated Chenopodium rubrum plants exposed to white, blue, and red light" in Russian Journal of Plant Physiology, 49, no. 4 (2002),
https://hdl.handle.net/21.15107/rcub_ibiss_1793 .

TY  - JOUR
AU  - Ilić, B.
AU  - Kostić, Elizabeta
AU  - Grubišić, Dragoljub V.
AU  - Ćulafić, Ljubinka
PY  - 2002
PY  - 2002
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/122
T2  - Arhiv za farmaciju
T1  - Umnožavanje izdanaka biljke Silybum marianum (L.) Gaertn. i njihov flavonoidni sastav
T1  - Multiplication of Silybum marianum (L.) Gaertn. shoots and their flavonoid composition
IS  - 4
VL  - 52
SP  - 806
EP  - 807
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_122
ER  - 

@article{
author = "Ilić, B. and Kostić, Elizabeta and Grubišić, Dragoljub V. and Ćulafić, Ljubinka",
year = "2002, 2002",
journal = "Arhiv za farmaciju",
title = "Umnožavanje izdanaka biljke Silybum marianum (L.) Gaertn. i njihov flavonoidni sastav, Multiplication of Silybum marianum (L.) Gaertn. shoots and their flavonoid composition",
number = "4",
volume = "52",
pages = "806-807",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_122"
}

Ilić, B., Kostić, E., Grubišić, D. V.,& Ćulafić, L.. (2002). Umnožavanje izdanaka biljke Silybum marianum (L.) Gaertn. i njihov flavonoidni sastav. in Arhiv za farmaciju, 52(4), 806-807.
https://hdl.handle.net/21.15107/rcub_ibiss_122

Ilić B, Kostić E, Grubišić DV, Ćulafić L. Umnožavanje izdanaka biljke Silybum marianum (L.) Gaertn. i njihov flavonoidni sastav. in Arhiv za farmaciju. 2002;52(4):806-807.
https://hdl.handle.net/21.15107/rcub_ibiss_122 .

Ilić, B., Kostić, Elizabeta, Grubišić, Dragoljub V., Ćulafić, Ljubinka, "Umnožavanje izdanaka biljke Silybum marianum (L.) Gaertn. i njihov flavonoidni sastav" in Arhiv za farmaciju, 52, no. 4 (2002):806-807,
https://hdl.handle.net/21.15107/rcub_ibiss_122 .

TY  - JOUR
AU  - Stojicić, Dragana D
AU  - Budimir, Snežana
AU  - Ćulafić, Ljubinka
PY  - 1999
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1851
AB  - Micropropagation by organogenesis from mature embryos of Pinus heldreichii Christ. was achieved. The frequency of adventitious bud induction was higher on embryos grown on Gresshoff and Doy medium than on Von Arnold and Eriksson, or Murashige and Skoog medium. The greatest number of buds and developed shoots was obtained after induction with benzyladenine at 2.22 or 4.40 mu M for four weeks. Shorter induction time was less effective for bud induction, but subsequent shoot elongation was accelerated. Shoots elongated on half-strength, growth regulator-free medium supplemented with activated charcoal. After pulse treatment with 1 mM indole-3-butyric acid twenty shoots were rooted, while agar-solidified medium supplemented with alpha-naphthaleneacetic acid (0.27 or 1.08 mu M), or indole-3-butyric acid (0.25 or 0.98 mu M) induced callus formation only.
T2  - Plant Cell Tissue and Organ Culture
T1  - Micropropagation of Pinus heldreichii
IS  - 2
VL  - 59
EP  - 150
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1851
ER  - 

@article{
author = "Stojicić, Dragana D and Budimir, Snežana and Ćulafić, Ljubinka",
year = "1999",
abstract = "Micropropagation by organogenesis from mature embryos of Pinus heldreichii Christ. was achieved. The frequency of adventitious bud induction was higher on embryos grown on Gresshoff and Doy medium than on Von Arnold and Eriksson, or Murashige and Skoog medium. The greatest number of buds and developed shoots was obtained after induction with benzyladenine at 2.22 or 4.40 mu M for four weeks. Shorter induction time was less effective for bud induction, but subsequent shoot elongation was accelerated. Shoots elongated on half-strength, growth regulator-free medium supplemented with activated charcoal. After pulse treatment with 1 mM indole-3-butyric acid twenty shoots were rooted, while agar-solidified medium supplemented with alpha-naphthaleneacetic acid (0.27 or 1.08 mu M), or indole-3-butyric acid (0.25 or 0.98 mu M) induced callus formation only.",
journal = "Plant Cell Tissue and Organ Culture",
title = "Micropropagation of Pinus heldreichii",
number = "2",
volume = "59",
pages = "150",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1851"
}

Stojicić, D. D., Budimir, S.,& Ćulafić, L.. (1999). Micropropagation of Pinus heldreichii. in Plant Cell Tissue and Organ Culture, 59(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1851

Stojicić DD, Budimir S, Ćulafić L. Micropropagation of Pinus heldreichii. in Plant Cell Tissue and Organ Culture. 1999;59(2):null-150.
https://hdl.handle.net/21.15107/rcub_ibiss_1851 .

Stojicić, Dragana D, Budimir, Snežana, Ćulafić, Ljubinka, "Micropropagation of Pinus heldreichii" in Plant Cell Tissue and Organ Culture, 59, no. 2 (1999),
https://hdl.handle.net/21.15107/rcub_ibiss_1851 .