TY  - JOUR
AU  - Rosić, Nedeljka
AU  - Momčilović, Ivana
AU  - Kovacević, Nada N
AU  - Grubišić, Dragoljub
PY  - 2006
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1623
AB  - Hairy roots of Rhamnus fallax Boiss. were induced using Agrobacterium rhizogenes strain A4M70GUS. The culture established on Woody plant media (WPM) showed a typical hairy root phenotype: rapid growth, reduced apical dominance and root plagiotropism. Seven clones of R. fallax were selected on the basis of their differences in colour and the root branching. The growth of hairy root culture, measured through gain in fresh mass, was done under 16-h photoperiod or in the dark. An increase in anthraquinone (AQ) content was obtained in clones with yellow and less branched roots, like clone 1 [16.43 mg g(-1)(d.m.)] and clone 7 [14.21 mg g(-1)(d.m.)], compared with other analysed transformed and non-transformed tissue. This study presents the first report of successful transformation of any species from family Rhamnaceae by A. rhizogenes and analysis of AQ production in transformed tissue.
T2  - Biologia Plantarum
T1  - Genetic transformation of Rhamnus fallax and hairy roots as a source of anthraquinones
IS  - 4
VL  - 50
EP  - 518
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1623
ER  - 

@article{
author = "Rosić, Nedeljka and Momčilović, Ivana and Kovacević, Nada N and Grubišić, Dragoljub",
year = "2006",
abstract = "Hairy roots of Rhamnus fallax Boiss. were induced using Agrobacterium rhizogenes strain A4M70GUS. The culture established on Woody plant media (WPM) showed a typical hairy root phenotype: rapid growth, reduced apical dominance and root plagiotropism. Seven clones of R. fallax were selected on the basis of their differences in colour and the root branching. The growth of hairy root culture, measured through gain in fresh mass, was done under 16-h photoperiod or in the dark. An increase in anthraquinone (AQ) content was obtained in clones with yellow and less branched roots, like clone 1 [16.43 mg g(-1)(d.m.)] and clone 7 [14.21 mg g(-1)(d.m.)], compared with other analysed transformed and non-transformed tissue. This study presents the first report of successful transformation of any species from family Rhamnaceae by A. rhizogenes and analysis of AQ production in transformed tissue.",
journal = "Biologia Plantarum",
title = "Genetic transformation of Rhamnus fallax and hairy roots as a source of anthraquinones",
number = "4",
volume = "50",
pages = "518",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1623"
}

Rosić, N., Momčilović, I., Kovacević, N. N.,& Grubišić, D.. (2006). Genetic transformation of Rhamnus fallax and hairy roots as a source of anthraquinones. in Biologia Plantarum, 50(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1623

Rosić N, Momčilović I, Kovacević NN, Grubišić D. Genetic transformation of Rhamnus fallax and hairy roots as a source of anthraquinones. in Biologia Plantarum. 2006;50(4):null-518.
https://hdl.handle.net/21.15107/rcub_ibiss_1623 .

Rosić, Nedeljka, Momčilović, Ivana, Kovacević, Nada N, Grubišić, Dragoljub, "Genetic transformation of Rhamnus fallax and hairy roots as a source of anthraquinones" in Biologia Plantarum, 50, no. 4 (2006),
https://hdl.handle.net/21.15107/rcub_ibiss_1623 .

TY  - JOUR
AU  - Kovacević, Nada N
AU  - Grubišić, Dragoljub
PY  - 2005
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1678
AB  - This study was conducted to investigate the effect of physical and chemical features on metabolic production in plant cell culture. The best multiplication of Frangula alnus Mill. shoots (8.84 +/- 1.11) was obtained on woody plant medium ( WPM) medium with indole-3-acetic acid (IAA) (0.1 mg l(-1)) and 6-benzylaminapurine (BAP) (1.0 mg l(-1)), but the highest metabolite production (1731 mg/100 g of total anthraquinone (AQ) aglycones) was in the shoots grown on the MS medium with addition of 1-naphthilaceneacetic (NAA) (0.1 mg l(-1)) and thidiazuron (TDZ) (0.1 mg l(-1)). The best multiplication of Frangula rupestris (Scop.) Schur. shoots (6.20 +/- 0.53) was obtained on MS medium supplemented with BAP (0.5 mg l(-1)). The highest metabolite production (451 mg/100 g of total anthraquinone aglycones) was in the shoots grown on medium with 2,4-D (0.1 mg l(-1)) and BAP (0.5 mg l(-1)). For the multiplication of Rhamnus catharticus L. shoots, the best results (2.8 +/- 0.7) were obtained on the medium with combination of 2,4-D (0.1 mg l(-1)) and kinetine (Kin) (1.0 mg l(-1)). The best synthesis of anthraquinones (AQs) (1205 mg/100 g of total AQ aglycones) was in the shoots, which were grown on MS medium supplemented with 2,4-D (0.1 mg l(-1)).
T2  - Pharmaceutical Biology
T1  - In vitro cultures of plants from the rhamnaceae: Shoot propagation and anthraquinones production
IS  - 5
VL  - 43
EP  - 424
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1678
ER  - 

@article{
author = "Kovacević, Nada N and Grubišić, Dragoljub",
year = "2005",
abstract = "This study was conducted to investigate the effect of physical and chemical features on metabolic production in plant cell culture. The best multiplication of Frangula alnus Mill. shoots (8.84 +/- 1.11) was obtained on woody plant medium ( WPM) medium with indole-3-acetic acid (IAA) (0.1 mg l(-1)) and 6-benzylaminapurine (BAP) (1.0 mg l(-1)), but the highest metabolite production (1731 mg/100 g of total anthraquinone (AQ) aglycones) was in the shoots grown on the MS medium with addition of 1-naphthilaceneacetic (NAA) (0.1 mg l(-1)) and thidiazuron (TDZ) (0.1 mg l(-1)). The best multiplication of Frangula rupestris (Scop.) Schur. shoots (6.20 +/- 0.53) was obtained on MS medium supplemented with BAP (0.5 mg l(-1)). The highest metabolite production (451 mg/100 g of total anthraquinone aglycones) was in the shoots grown on medium with 2,4-D (0.1 mg l(-1)) and BAP (0.5 mg l(-1)). For the multiplication of Rhamnus catharticus L. shoots, the best results (2.8 +/- 0.7) were obtained on the medium with combination of 2,4-D (0.1 mg l(-1)) and kinetine (Kin) (1.0 mg l(-1)). The best synthesis of anthraquinones (AQs) (1205 mg/100 g of total AQ aglycones) was in the shoots, which were grown on MS medium supplemented with 2,4-D (0.1 mg l(-1)).",
journal = "Pharmaceutical Biology",
title = "In vitro cultures of plants from the rhamnaceae: Shoot propagation and anthraquinones production",
number = "5",
volume = "43",
pages = "424",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1678"
}

Kovacević, N. N.,& Grubišić, D.. (2005). In vitro cultures of plants from the rhamnaceae: Shoot propagation and anthraquinones production. in Pharmaceutical Biology, 43(5).
https://hdl.handle.net/21.15107/rcub_ibiss_1678

Kovacević NN, Grubišić D. In vitro cultures of plants from the rhamnaceae: Shoot propagation and anthraquinones production. in Pharmaceutical Biology. 2005;43(5):null-424.
https://hdl.handle.net/21.15107/rcub_ibiss_1678 .

Kovacević, Nada N, Grubišić, Dragoljub, "In vitro cultures of plants from the rhamnaceae: Shoot propagation and anthraquinones production" in Pharmaceutical Biology, 43, no. 5 (2005),
https://hdl.handle.net/21.15107/rcub_ibiss_1678 .

TY  - JOUR
AU  - Grubišić, Dragoljub
AU  - Kovacević, Nada N
AU  - Ristić, Mihailo S.
AU  - Tasić, Slavoljub R
AU  - Menković, Nebojsa R
AU  - Đoković, Dejan D
PY  - 2005
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1721
AB  - The composition of the essential oils from herbs of Achillea alexandri-regis, A. holosericea and A. lingulata was analyzed by GC and GUMS. The predominant compounds in the oil of A. alexandri-regis were: alpha-pinene (14.4-15.7%), isopinocamphone (5.4-23.5%), alpha-phellandrene epoxide (5.0-19.0%), borneol (3.6-7.7%) and spathulenol (1.4-9.7%). The major components in the herb oil of A. holosericea were: borneol (17.3-18.1%), camphor (9.7-15.1%). terpinen-4-ol (6.3-11.1%) and camphene (5.7-7.2%). The presence of borneol (23.040.7%), beta-thujone (5.5-7.6%). bornyl acetate (3.5-7.8%) and sabinene (1.7-6.7%) was characteristic of A. lingulata herb oils. The presence of borneol in high concentration was a connecting property for the oil of all three investigated Achillea species, especially for A. lingulata and A. holosericea. On the other side, the low concentration (0.2%) of camphor was characteristic of the investigated samples of A. alexandri-regis and A. lingulata.
T2  - Journal of Essential Oil Research
T1  - Comparative study of essential oil of three Achillea species from Serbia
IS  - 1
VL  - 17
EP  - 60
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1721
ER  - 

@article{
author = "Grubišić, Dragoljub and Kovacević, Nada N and Ristić, Mihailo S. and Tasić, Slavoljub R and Menković, Nebojsa R and Đoković, Dejan D",
year = "2005",
abstract = "The composition of the essential oils from herbs of Achillea alexandri-regis, A. holosericea and A. lingulata was analyzed by GC and GUMS. The predominant compounds in the oil of A. alexandri-regis were: alpha-pinene (14.4-15.7%), isopinocamphone (5.4-23.5%), alpha-phellandrene epoxide (5.0-19.0%), borneol (3.6-7.7%) and spathulenol (1.4-9.7%). The major components in the herb oil of A. holosericea were: borneol (17.3-18.1%), camphor (9.7-15.1%). terpinen-4-ol (6.3-11.1%) and camphene (5.7-7.2%). The presence of borneol (23.040.7%), beta-thujone (5.5-7.6%). bornyl acetate (3.5-7.8%) and sabinene (1.7-6.7%) was characteristic of A. lingulata herb oils. The presence of borneol in high concentration was a connecting property for the oil of all three investigated Achillea species, especially for A. lingulata and A. holosericea. On the other side, the low concentration (0.2%) of camphor was characteristic of the investigated samples of A. alexandri-regis and A. lingulata.",
journal = "Journal of Essential Oil Research",
title = "Comparative study of essential oil of three Achillea species from Serbia",
number = "1",
volume = "17",
pages = "60",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1721"
}

Grubišić, D., Kovacević, N. N., Ristić, M. S., Tasić, S. R., Menković, N. R.,& Đoković, D. D.. (2005). Comparative study of essential oil of three Achillea species from Serbia. in Journal of Essential Oil Research, 17(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1721

Grubišić D, Kovacević NN, Ristić MS, Tasić SR, Menković NR, Đoković DD. Comparative study of essential oil of three Achillea species from Serbia. in Journal of Essential Oil Research. 2005;17(1):null-60.
https://hdl.handle.net/21.15107/rcub_ibiss_1721 .

Grubišić, Dragoljub, Kovacević, Nada N, Ristić, Mihailo S., Tasić, Slavoljub R, Menković, Nebojsa R, Đoković, Dejan D, "Comparative study of essential oil of three Achillea species from Serbia" in Journal of Essential Oil Research, 17, no. 1 (2005),
https://hdl.handle.net/21.15107/rcub_ibiss_1721 .

TY  - JOUR
AU  - Ristić, Mihailo S.
AU  - Soković, Marina
AU  - Grubišić, Dragoljub
AU  - Kovacević, Nada N
PY  - 2004
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1755
AB  - The essential oil of Achillea atrata L. and its major component, 1,8-cineole, were evaluated for their antifungal activity by the mycelial growth method against 18 micromycetes (Alternaria alternata, Aspergillus niger, A. ochraceus, A. versicolor, A. flavus, A. terreus, Cladosporium cladosporioides, Fusarium tricinctum, Penicillium ochrochloron, P. funiculosum, Phomopsis helianthi, Trichoderma viride, Trichophyton mentagrophytes, T. rubrum, T tonsurans, Epidermophyton floccosum, Microsporum canis, M. gypseum). The oil exhibited strong inhibitory action against all tested micromycetes with MIC values of 2.0-8.0 muL/mL.
T2  - Journal of Essential Oil Research
T1  - Chemical analysis and antifungal activity of the essential oil of Achillea atrata L.
IS  - 1
VL  - 16
EP  - 78
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1755
ER  - 

@article{
author = "Ristić, Mihailo S. and Soković, Marina and Grubišić, Dragoljub and Kovacević, Nada N",
year = "2004",
abstract = "The essential oil of Achillea atrata L. and its major component, 1,8-cineole, were evaluated for their antifungal activity by the mycelial growth method against 18 micromycetes (Alternaria alternata, Aspergillus niger, A. ochraceus, A. versicolor, A. flavus, A. terreus, Cladosporium cladosporioides, Fusarium tricinctum, Penicillium ochrochloron, P. funiculosum, Phomopsis helianthi, Trichoderma viride, Trichophyton mentagrophytes, T. rubrum, T tonsurans, Epidermophyton floccosum, Microsporum canis, M. gypseum). The oil exhibited strong inhibitory action against all tested micromycetes with MIC values of 2.0-8.0 muL/mL.",
journal = "Journal of Essential Oil Research",
title = "Chemical analysis and antifungal activity of the essential oil of Achillea atrata L.",
number = "1",
volume = "16",
pages = "78",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1755"
}

Ristić, M. S., Soković, M., Grubišić, D.,& Kovacević, N. N.. (2004). Chemical analysis and antifungal activity of the essential oil of Achillea atrata L.. in Journal of Essential Oil Research, 16(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1755

Ristić MS, Soković M, Grubišić D, Kovacević NN. Chemical analysis and antifungal activity of the essential oil of Achillea atrata L.. in Journal of Essential Oil Research. 2004;16(1):null-78.
https://hdl.handle.net/21.15107/rcub_ibiss_1755 .

Ristić, Mihailo S., Soković, Marina, Grubišić, Dragoljub, Kovacević, Nada N, "Chemical analysis and antifungal activity of the essential oil of Achillea atrata L." in Journal of Essential Oil Research, 16, no. 1 (2004),
https://hdl.handle.net/21.15107/rcub_ibiss_1755 .