TY  - CONF
AU  - Stanković, Nevena
AU  - Mladenović, Milan
AU  - Matić, Sanja
AU  - Stanić, Snežana
AU  - Mihailović, Mirjana
AU  - Mihailović, Vladimir
AU  - Katanić, Jelena
AU  - Boroja, Tatjana
AU  - Vuković, Nenad
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6144
AB  - Eight chroman-2,4-diones, namely 2a-h, were evaluated as in vivo genotoxic agents in Wistar rats livers and kidneys using the alkaline comet assay. Compounds 2a, (E)-3- (1-(2-aminoethylamino) ethylidene) chroman-2,4-dione,2b,(E)-3-( 1-(2-hydroxyethylamino) ethylidene) chroman-2,4-dione, and 2f, (3E,3'E) - 3,3'-( l, l '-(ethane-1,2-diylbis (azanediyl)) bis (ethan-1-yl-l-ylidene)) dichroman-2,4- dione showed no genotoxic potential and were tested as antigenotoxic agents by application prior to ethyl methanesulfonate (EMS), a proven mutagen. As antigentotoxics, compounds significantly diminished EMS-induced DNA damage in both organs. The reduction of liver DNA damage amounted 86.93% (2b), 77.23% (2f), and 64.52% (2a), respectively, while the reduction in kidney DNA damage was 89.52 (2b), 82.50% (2f) and 68.14% (2a). Since EMS produce harmful d-ethylguanine lesion which is incorporated in aberrant genotoxic G=T and T=G pairing after rat Topoisomerase Ila (rToplla) catalyzed ATP-dependent DNA strand breaks, the mechanism of 2a, 2b, and 2f antigenotoxic activity was investigated on enzyme level using molecular docking and molecular dynamics simulations. According to molecular docking studies, those compounds occupy the A TPase region proximal to rGlu86, catalytic amino acid involved in the hydrolysis of y-pbosphate group of ATP via water bridges. Molecular dynamics simulations showed that 2a, 2b, and 2f are a barrier for the formation of ATP-H20-rGlu86 bridge. Since compounds inhibit the hydrolysis of ATP, they prohibit the energy for the DNA double strand ligation, and therefore neutralize any possible damage that can arise after the formation of 06-ethylguanine harmful lesion. Consequently, compounds 2a, 2b, and 2f prevent EMS mutagenic and carcinogenic effects, and can be applied in the cancer treatment to control the rate of anticancer alkylation drugs.
PB  - Banja Luka: Prirodno-matematički fakultet
C3  - III simpozijum biologa i ekologa Republike Srpske (SBERS, 2015): Zbornik radova; 2015 Nov 12-14; Banja Luka, Republika Srpska
T1  - Newly discovered chroman-2,4-diones neutralize DNA alkylation damage in vivo on topIIa level: A story behind the molecular modeling approach
SP  - 118
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6144
ER  - 

@conference{
author = "Stanković, Nevena and Mladenović, Milan and Matić, Sanja and Stanić, Snežana and Mihailović, Mirjana and Mihailović, Vladimir and Katanić, Jelena and Boroja, Tatjana and Vuković, Nenad",
year = "2015",
abstract = "Eight chroman-2,4-diones, namely 2a-h, were evaluated as in vivo genotoxic agents in Wistar rats livers and kidneys using the alkaline comet assay. Compounds 2a, (E)-3- (1-(2-aminoethylamino) ethylidene) chroman-2,4-dione,2b,(E)-3-( 1-(2-hydroxyethylamino) ethylidene) chroman-2,4-dione, and 2f, (3E,3'E) - 3,3'-( l, l '-(ethane-1,2-diylbis (azanediyl)) bis (ethan-1-yl-l-ylidene)) dichroman-2,4- dione showed no genotoxic potential and were tested as antigenotoxic agents by application prior to ethyl methanesulfonate (EMS), a proven mutagen. As antigentotoxics, compounds significantly diminished EMS-induced DNA damage in both organs. The reduction of liver DNA damage amounted 86.93% (2b), 77.23% (2f), and 64.52% (2a), respectively, while the reduction in kidney DNA damage was 89.52 (2b), 82.50% (2f) and 68.14% (2a). Since EMS produce harmful d-ethylguanine lesion which is incorporated in aberrant genotoxic G=T and T=G pairing after rat Topoisomerase Ila (rToplla) catalyzed ATP-dependent DNA strand breaks, the mechanism of 2a, 2b, and 2f antigenotoxic activity was investigated on enzyme level using molecular docking and molecular dynamics simulations. According to molecular docking studies, those compounds occupy the A TPase region proximal to rGlu86, catalytic amino acid involved in the hydrolysis of y-pbosphate group of ATP via water bridges. Molecular dynamics simulations showed that 2a, 2b, and 2f are a barrier for the formation of ATP-H20-rGlu86 bridge. Since compounds inhibit the hydrolysis of ATP, they prohibit the energy for the DNA double strand ligation, and therefore neutralize any possible damage that can arise after the formation of 06-ethylguanine harmful lesion. Consequently, compounds 2a, 2b, and 2f prevent EMS mutagenic and carcinogenic effects, and can be applied in the cancer treatment to control the rate of anticancer alkylation drugs.",
publisher = "Banja Luka: Prirodno-matematički fakultet",
journal = "III simpozijum biologa i ekologa Republike Srpske (SBERS, 2015): Zbornik radova; 2015 Nov 12-14; Banja Luka, Republika Srpska",
title = "Newly discovered chroman-2,4-diones neutralize DNA alkylation damage in vivo on topIIa level: A story behind the molecular modeling approach",
pages = "118",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6144"
}

Stanković, N., Mladenović, M., Matić, S., Stanić, S., Mihailović, M., Mihailović, V., Katanić, J., Boroja, T.,& Vuković, N.. (2015). Newly discovered chroman-2,4-diones neutralize DNA alkylation damage in vivo on topIIa level: A story behind the molecular modeling approach. in III simpozijum biologa i ekologa Republike Srpske (SBERS, 2015): Zbornik radova; 2015 Nov 12-14; Banja Luka, Republika Srpska
Banja Luka: Prirodno-matematički fakultet., 118.
https://hdl.handle.net/21.15107/rcub_ibiss_6144

Stanković N, Mladenović M, Matić S, Stanić S, Mihailović M, Mihailović V, Katanić J, Boroja T, Vuković N. Newly discovered chroman-2,4-diones neutralize DNA alkylation damage in vivo on topIIa level: A story behind the molecular modeling approach. in III simpozijum biologa i ekologa Republike Srpske (SBERS, 2015): Zbornik radova; 2015 Nov 12-14; Banja Luka, Republika Srpska. 2015;:118.
https://hdl.handle.net/21.15107/rcub_ibiss_6144 .

Stanković, Nevena, Mladenović, Milan, Matić, Sanja, Stanić, Snežana, Mihailović, Mirjana, Mihailović, Vladimir, Katanić, Jelena, Boroja, Tatjana, Vuković, Nenad, "Newly discovered chroman-2,4-diones neutralize DNA alkylation damage in vivo on topIIa level: A story behind the molecular modeling approach" in III simpozijum biologa i ekologa Republike Srpske (SBERS, 2015): Zbornik radova; 2015 Nov 12-14; Banja Luka, Republika Srpska (2015):118,
https://hdl.handle.net/21.15107/rcub_ibiss_6144 .

TY  - JOUR
AU  - Stanković, Nevena
AU  - Mladenović, Milan
AU  - Matić, Sanja
AU  - Stanić, Snežana
AU  - Stanković, Vesna
AU  - Mihailović, Mirjana
AU  - Mihailović, Vladimir
AU  - Katanić, Jelena
AU  - Boroja, Tatjana
AU  - Vuković, Nenad
AU  - Sukdolak, Slobodan
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2004
AB  - Two chroman-2,4-dione derivatives, namely 2a and 2f, were tested as in
   vivo anticoagulants by seven days of continuous per os application to
   adult male Wistar rats in a concentration of 20 mg/kg of body weight.
   Derivatives were selected from a group of six previously
   intraperitoneally applied compounds on the basis of presenting
   remarkable activity in a concentration of 2 mg/kg of body weight. The
   derivatives 2a and 2f are VKORC1 inhibitors, and comparison of the
   absorption spectra, association, and dissociation constants suggested
   that the compounds will be bound to serum albumin in the same manner as
   warfarin is, leading to transfer towards the molecular target VKORC1.
   After oral administration, the compounds proved to be anticoagulants
   comparable with warfarin, inasmuch as the measured prothrombin times for
   2a and 2f were 56.63 and 60.08 s, respectively. The INR values of 2a and
   2f ranged from 2.6 to 2.8, recommending them as useful therapeutics in
   the treatment of patients suffering from thromboembolic events and
   atrial fibrillation. The high percentage of binding and high binding
   affinity of 2a and 2f towards serum albumin reduced the risk of induced
   internal bleeding. Several kinds of toxicity studies were performed to
   investigate whether or not 2a and 2f can cause pathological changes in
   the liver, kidneys, and DNA. The catalytic activity of serum enzymes,
   concentration and catalytic activity of liver and kidney oxidative
   stress markers and enzymes, respectively, as well as the observed
   hepatic and renal morphological changes indicated that the compounds in
   relation to warfarin induced irrelevant hepatic toxicity, no increment
   of necrosis, and inconsiderable oxidative damage in the liver and
   kidneys. Estimation of DNA damage using the comet assay confirmed that
   2a and 2f caused no clinically significant genotoxicity. The higher
   activity and lower toxicity of 2f recommended this compound as a better
   drug candidate than 2a. (C) 2014 Elsevier Ireland Ltd. All rights
   reserved.
PB  - Elsevier
T2  - Chemico-Biological Interactions
T1  - Serum albumin binding analysis and toxicological screening of novel
 chroman-2,4-diones as oral anticoagulants
VL  - 227
DO  - 10.1016/j.cbi.2014.12.005
SP  - 18
EP  - 31
ER  - 

@article{
author = "Stanković, Nevena and Mladenović, Milan and Matić, Sanja and Stanić, Snežana and Stanković, Vesna and Mihailović, Mirjana and Mihailović, Vladimir and Katanić, Jelena and Boroja, Tatjana and Vuković, Nenad and Sukdolak, Slobodan",
year = "2015",
abstract = "Two chroman-2,4-dione derivatives, namely 2a and 2f, were tested as in
   vivo anticoagulants by seven days of continuous per os application to
   adult male Wistar rats in a concentration of 20 mg/kg of body weight.
   Derivatives were selected from a group of six previously
   intraperitoneally applied compounds on the basis of presenting
   remarkable activity in a concentration of 2 mg/kg of body weight. The
   derivatives 2a and 2f are VKORC1 inhibitors, and comparison of the
   absorption spectra, association, and dissociation constants suggested
   that the compounds will be bound to serum albumin in the same manner as
   warfarin is, leading to transfer towards the molecular target VKORC1.
   After oral administration, the compounds proved to be anticoagulants
   comparable with warfarin, inasmuch as the measured prothrombin times for
   2a and 2f were 56.63 and 60.08 s, respectively. The INR values of 2a and
   2f ranged from 2.6 to 2.8, recommending them as useful therapeutics in
   the treatment of patients suffering from thromboembolic events and
   atrial fibrillation. The high percentage of binding and high binding
   affinity of 2a and 2f towards serum albumin reduced the risk of induced
   internal bleeding. Several kinds of toxicity studies were performed to
   investigate whether or not 2a and 2f can cause pathological changes in
   the liver, kidneys, and DNA. The catalytic activity of serum enzymes,
   concentration and catalytic activity of liver and kidney oxidative
   stress markers and enzymes, respectively, as well as the observed
   hepatic and renal morphological changes indicated that the compounds in
   relation to warfarin induced irrelevant hepatic toxicity, no increment
   of necrosis, and inconsiderable oxidative damage in the liver and
   kidneys. Estimation of DNA damage using the comet assay confirmed that
   2a and 2f caused no clinically significant genotoxicity. The higher
   activity and lower toxicity of 2f recommended this compound as a better
   drug candidate than 2a. (C) 2014 Elsevier Ireland Ltd. All rights
   reserved.",
publisher = "Elsevier",
journal = "Chemico-Biological Interactions",
title = "Serum albumin binding analysis and toxicological screening of novel
 chroman-2,4-diones as oral anticoagulants",
volume = "227",
doi = "10.1016/j.cbi.2014.12.005",
pages = "18-31"
}

Stanković, N., Mladenović, M., Matić, S., Stanić, S., Stanković, V., Mihailović, M., Mihailović, V., Katanić, J., Boroja, T., Vuković, N.,& Sukdolak, S.. (2015). Serum albumin binding analysis and toxicological screening of novel
 chroman-2,4-diones as oral anticoagulants. in Chemico-Biological Interactions
Elsevier., 227, 18-31.
https://doi.org/10.1016/j.cbi.2014.12.005

Stanković N, Mladenović M, Matić S, Stanić S, Stanković V, Mihailović M, Mihailović V, Katanić J, Boroja T, Vuković N, Sukdolak S. Serum albumin binding analysis and toxicological screening of novel
 chroman-2,4-diones as oral anticoagulants. in Chemico-Biological Interactions. 2015;227:18-31.
doi:10.1016/j.cbi.2014.12.005 .

Stanković, Nevena, Mladenović, Milan, Matić, Sanja, Stanić, Snežana, Stanković, Vesna, Mihailović, Mirjana, Mihailović, Vladimir, Katanić, Jelena, Boroja, Tatjana, Vuković, Nenad, Sukdolak, Slobodan, "Serum albumin binding analysis and toxicological screening of novel
 chroman-2,4-diones as oral anticoagulants" in Chemico-Biological Interactions, 227 (2015):18-31,
https://doi.org/10.1016/j.cbi.2014.12.005 . .

TY  - JOUR
AU  - Mihailović, Vladimir
AU  - Mišić, Danijela
AU  - Matić, Sanja
AU  - Mihailović, Mirjana
AU  - Stanić, Snežana
AU  - Vrvić, Miroslav M.
AU  - Katanić, Jelena
AU  - Mladenović, Milan
AU  - Stanković, Nevena
AU  - Boroja, Tatjana
AU  - Stanković, Milan S.
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2350
AB  - The purpose of this study was to evaluate the antioxidant potential of
   methanol extracts of Gentiana crudata L. aerial parts and roots, as well
   as the stability of the phenolic compounds and antioxidant capacity of
   extracts during heating, at different pHs and after an in vitro
   digestion procedure. Also, their genotoxicity and antigenotoxicity
   against carbon tetrachloride in the liver of albino Wistar rats using
   the comet assay were evaluated. Three secoiridoid glycosides
   (swertiamarin, gentiopicrin, and sweroside) and four phenolic compounds
   (orientin, vitexin and two isovitexin-glucosides) were identified as the
   major constituents in aerial parts and roots of G. cruciata, using
   UHPLC-DAD/+/- HESI-MS/MS analysis. The results of antioxidant assays
   showed that aerial parts displayed higher antioxidant activity compared
   to the roots, which could be related to higher phenolics content,
   especially flavonoids. In general, extracts showed pH and thermal
   stability, while duodenal condition had more influence on total phenolic
   condition and antioxidant activity of extracts. Both extracts showed a
   protective effect against CCl4 in comet assays. The roots extract showed
   no genotoxic activity, while aerial parts extract showed slight
   genotoxicity at concentrations of 400 mg/kg b.w. (C) 2015 Elsevier B.V.
   All rights reserved.
PB  - Elsevier
T2  - Industrial Crops and Products
T1  - Comparative phytochemical analysis of Gentiana cruciata L. roots and
 aerial parts, and their biological activities
VL  - 73
DO  - 10.1016/j.indcrop.2015.04.013
SP  - 49
EP  - 62
ER  - 

@article{
author = "Mihailović, Vladimir and Mišić, Danijela and Matić, Sanja and Mihailović, Mirjana and Stanić, Snežana and Vrvić, Miroslav M. and Katanić, Jelena and Mladenović, Milan and Stanković, Nevena and Boroja, Tatjana and Stanković, Milan S.",
year = "2015",
abstract = "The purpose of this study was to evaluate the antioxidant potential of
   methanol extracts of Gentiana crudata L. aerial parts and roots, as well
   as the stability of the phenolic compounds and antioxidant capacity of
   extracts during heating, at different pHs and after an in vitro
   digestion procedure. Also, their genotoxicity and antigenotoxicity
   against carbon tetrachloride in the liver of albino Wistar rats using
   the comet assay were evaluated. Three secoiridoid glycosides
   (swertiamarin, gentiopicrin, and sweroside) and four phenolic compounds
   (orientin, vitexin and two isovitexin-glucosides) were identified as the
   major constituents in aerial parts and roots of G. cruciata, using
   UHPLC-DAD/+/- HESI-MS/MS analysis. The results of antioxidant assays
   showed that aerial parts displayed higher antioxidant activity compared
   to the roots, which could be related to higher phenolics content,
   especially flavonoids. In general, extracts showed pH and thermal
   stability, while duodenal condition had more influence on total phenolic
   condition and antioxidant activity of extracts. Both extracts showed a
   protective effect against CCl4 in comet assays. The roots extract showed
   no genotoxic activity, while aerial parts extract showed slight
   genotoxicity at concentrations of 400 mg/kg b.w. (C) 2015 Elsevier B.V.
   All rights reserved.",
publisher = "Elsevier",
journal = "Industrial Crops and Products",
title = "Comparative phytochemical analysis of Gentiana cruciata L. roots and
 aerial parts, and their biological activities",
volume = "73",
doi = "10.1016/j.indcrop.2015.04.013",
pages = "49-62"
}

Mihailović, V., Mišić, D., Matić, S., Mihailović, M., Stanić, S., Vrvić, M. M., Katanić, J., Mladenović, M., Stanković, N., Boroja, T.,& Stanković, M. S.. (2015). Comparative phytochemical analysis of Gentiana cruciata L. roots and
 aerial parts, and their biological activities. in Industrial Crops and Products
Elsevier., 73, 49-62.
https://doi.org/10.1016/j.indcrop.2015.04.013

Mihailović V, Mišić D, Matić S, Mihailović M, Stanić S, Vrvić MM, Katanić J, Mladenović M, Stanković N, Boroja T, Stanković MS. Comparative phytochemical analysis of Gentiana cruciata L. roots and
 aerial parts, and their biological activities. in Industrial Crops and Products. 2015;73:49-62.
doi:10.1016/j.indcrop.2015.04.013 .

Mihailović, Vladimir, Mišić, Danijela, Matić, Sanja, Mihailović, Mirjana, Stanić, Snežana, Vrvić, Miroslav M., Katanić, Jelena, Mladenović, Milan, Stanković, Nevena, Boroja, Tatjana, Stanković, Milan S., "Comparative phytochemical analysis of Gentiana cruciata L. roots and
 aerial parts, and their biological activities" in Industrial Crops and Products, 73 (2015):49-62,
https://doi.org/10.1016/j.indcrop.2015.04.013 . .

TY  - JOUR
AU  - Mihailović, Vladimir
AU  - Katanić, Jelena
AU  - Mišić, Danijela
AU  - Stanković, Vesna
AU  - Mihailović, Mirjana
AU  - Uskoković, Aleksandra
AU  - Arambašić Jovanović, Jelena
AU  - Solujić, Slavica
AU  - Mladenović, Milan
AU  - Stanković, Nevena
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2179
AB  - The objective of this work was to investigate the effects of the
   methanol extracts of Gentiana cruciata L. aerial parts (GCA) and roots
   (GCR) against carbon tetrachloride-induced liver injury in rats.
   Pretreatment with GCA and GCR, containing sweroside, swertiamarin and
   gentiopicrin in high concentrations, dose-dependently and significantly
   decreased the levels of serum transaminases, alkaline phosphatase and
   total bilirubin, whereas an increase in the level of total protein was
   found compared with the CCl4-treated group. Moreover, oral
   administration of extracts significantly enhanced antioxidant enzyme
   activities (superoxide dismutase and catalase), increased the content of
   glutathione and decreased the content of TBARS. Microscopic evaluations
   of the liver revealed CCl4-induced lesions and related toxic
   manifestations that were minimal in the liver of rats pretreated with
   extracts at the dose of 400 mg per kg b.w. The results suggest that the
   use of G. cruciata extracts has a merit as a potent candidate in
   protecting the liver against chemical induced toxicity.
PB  - The Royal Society of Chemistry
T2  - Food and Function
T1  - Hepatoprotective effects of secoiridoid-rich extracts from Gentiana
 cruciata L. against carbon tetrachloride induced liver damage in rats
IS  - 8
VL  - 5
DO  - 10.1039/c4fo00088a
SP  - 1795
EP  - 1803
ER  - 

@article{
author = "Mihailović, Vladimir and Katanić, Jelena and Mišić, Danijela and Stanković, Vesna and Mihailović, Mirjana and Uskoković, Aleksandra and Arambašić Jovanović, Jelena and Solujić, Slavica and Mladenović, Milan and Stanković, Nevena",
year = "2014",
abstract = "The objective of this work was to investigate the effects of the
   methanol extracts of Gentiana cruciata L. aerial parts (GCA) and roots
   (GCR) against carbon tetrachloride-induced liver injury in rats.
   Pretreatment with GCA and GCR, containing sweroside, swertiamarin and
   gentiopicrin in high concentrations, dose-dependently and significantly
   decreased the levels of serum transaminases, alkaline phosphatase and
   total bilirubin, whereas an increase in the level of total protein was
   found compared with the CCl4-treated group. Moreover, oral
   administration of extracts significantly enhanced antioxidant enzyme
   activities (superoxide dismutase and catalase), increased the content of
   glutathione and decreased the content of TBARS. Microscopic evaluations
   of the liver revealed CCl4-induced lesions and related toxic
   manifestations that were minimal in the liver of rats pretreated with
   extracts at the dose of 400 mg per kg b.w. The results suggest that the
   use of G. cruciata extracts has a merit as a potent candidate in
   protecting the liver against chemical induced toxicity.",
publisher = "The Royal Society of Chemistry",
journal = "Food and Function",
title = "Hepatoprotective effects of secoiridoid-rich extracts from Gentiana
 cruciata L. against carbon tetrachloride induced liver damage in rats",
number = "8",
volume = "5",
doi = "10.1039/c4fo00088a",
pages = "1795-1803"
}

Mihailović, V., Katanić, J., Mišić, D., Stanković, V., Mihailović, M., Uskoković, A., Arambašić Jovanović, J., Solujić, S., Mladenović, M.,& Stanković, N.. (2014). Hepatoprotective effects of secoiridoid-rich extracts from Gentiana
 cruciata L. against carbon tetrachloride induced liver damage in rats. in Food and Function
The Royal Society of Chemistry., 5(8), 1795-1803.
https://doi.org/10.1039/c4fo00088a

Mihailović V, Katanić J, Mišić D, Stanković V, Mihailović M, Uskoković A, Arambašić Jovanović J, Solujić S, Mladenović M, Stanković N. Hepatoprotective effects of secoiridoid-rich extracts from Gentiana
 cruciata L. against carbon tetrachloride induced liver damage in rats. in Food and Function. 2014;5(8):1795-1803.
doi:10.1039/c4fo00088a .

Mihailović, Vladimir, Katanić, Jelena, Mišić, Danijela, Stanković, Vesna, Mihailović, Mirjana, Uskoković, Aleksandra, Arambašić Jovanović, Jelena, Solujić, Slavica, Mladenović, Milan, Stanković, Nevena, "Hepatoprotective effects of secoiridoid-rich extracts from Gentiana
 cruciata L. against carbon tetrachloride induced liver damage in rats" in Food and Function, 5, no. 8 (2014):1795-1803,
https://doi.org/10.1039/c4fo00088a . .

TY  - CONF
AU  - Matić, Sanja
AU  - Stanić, Snežana
AU  - Bogojević, Desanka
AU  - Solujić, Slavica
AU  - Mladenović, Milan
AU  - Stanković, Nevena
AU  - Mihailović, Vladimir
AU  - Katanić, Jelena
AU  - Mihailović, Mirjana
PY  - 2013
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6141
AB  - The plant Cotinus coggygria Scop. (family Anacardiaceae) is commonly used in folk medicine for the treat­ment of various illnesses, such as diarrhea, paradontosis, gastric and duodenal ulcers. Different parts of this plant have been subjected to pharmacological evaluation for their potential antihaemorragic, wound-healing, anti­inflammatory, and antimicrobial activity. The present study was undertaken to investigate the phenolic conten according to the Folin-Ciocalteu procedure, while the spectrophotometric method with aluminium chloride was used for the determination of total ftavonoids. The phenolic and flavonoid composition of extract were deter­mined by the HPLC method. Antioxidant activiy was quantitatively determined using a DPPH radical scaveng­ing assay. To examine the antigenotoxic potential using the comet assay, Wistar rats were treated with 100 mg/ kg body weight of pyrogallol, which possesses a potent ability to generate free radicals and induce oxidative stress. The content of total phenols and ftavonoids was 3.78 mg of gallic acid/g of dry plant material and 8.29 mg of rutin/g of dry plant material, respectively. HPLC analysis showed that myricetin was the dominant compound (511.5 µg/g), while hydroxyl derivatives of cinnammic acids (chlorogenic, caffeic, coumaric, ferulic and rosmaric acid) were identified in the extract in varying amount. The neutralisation of DPPH radicals was up to 95%, while the maximum inhibition concentration is approximately 125 µg/ml. The dose of 500 mg/kg body weight of the extract, that display no genotoxic activity, and its main flavonoid compound myricetin, in equivalent amount as present in the extract, were applied intraperitoneally either 2 or 12 h prior to pyrogallol. As measured by the decrease in total score and tail moment, the DNA damage in liver was reduced by the extract and myricetin.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia
T1  - Chemical composition, antioxidant and antigenotoxic activities of Cotinus coggygria stem extract
SP  - 90
EP  - 91
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6141
ER  - 

@conference{
author = "Matić, Sanja and Stanić, Snežana and Bogojević, Desanka and Solujić, Slavica and Mladenović, Milan and Stanković, Nevena and Mihailović, Vladimir and Katanić, Jelena and Mihailović, Mirjana",
year = "2013",
abstract = "The plant Cotinus coggygria Scop. (family Anacardiaceae) is commonly used in folk medicine for the treat­ment of various illnesses, such as diarrhea, paradontosis, gastric and duodenal ulcers. Different parts of this plant have been subjected to pharmacological evaluation for their potential antihaemorragic, wound-healing, anti­inflammatory, and antimicrobial activity. The present study was undertaken to investigate the phenolic conten according to the Folin-Ciocalteu procedure, while the spectrophotometric method with aluminium chloride was used for the determination of total ftavonoids. The phenolic and flavonoid composition of extract were deter­mined by the HPLC method. Antioxidant activiy was quantitatively determined using a DPPH radical scaveng­ing assay. To examine the antigenotoxic potential using the comet assay, Wistar rats were treated with 100 mg/ kg body weight of pyrogallol, which possesses a potent ability to generate free radicals and induce oxidative stress. The content of total phenols and ftavonoids was 3.78 mg of gallic acid/g of dry plant material and 8.29 mg of rutin/g of dry plant material, respectively. HPLC analysis showed that myricetin was the dominant compound (511.5 µg/g), while hydroxyl derivatives of cinnammic acids (chlorogenic, caffeic, coumaric, ferulic and rosmaric acid) were identified in the extract in varying amount. The neutralisation of DPPH radicals was up to 95%, while the maximum inhibition concentration is approximately 125 µg/ml. The dose of 500 mg/kg body weight of the extract, that display no genotoxic activity, and its main flavonoid compound myricetin, in equivalent amount as present in the extract, were applied intraperitoneally either 2 or 12 h prior to pyrogallol. As measured by the decrease in total score and tail moment, the DNA damage in liver was reduced by the extract and myricetin.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia",
title = "Chemical composition, antioxidant and antigenotoxic activities of Cotinus coggygria stem extract",
pages = "90-91",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6141"
}

Matić, S., Stanić, S., Bogojević, D., Solujić, S., Mladenović, M., Stanković, N., Mihailović, V., Katanić, J.,& Mihailović, M.. (2013). Chemical composition, antioxidant and antigenotoxic activities of Cotinus coggygria stem extract. in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia
Belgrade: Serbian Plant Physiology Society., 90-91.
https://hdl.handle.net/21.15107/rcub_ibiss_6141

Matić S, Stanić S, Bogojević D, Solujić S, Mladenović M, Stanković N, Mihailović V, Katanić J, Mihailović M. Chemical composition, antioxidant and antigenotoxic activities of Cotinus coggygria stem extract. in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia. 2013;:90-91.
https://hdl.handle.net/21.15107/rcub_ibiss_6141 .

Matić, Sanja, Stanić, Snežana, Bogojević, Desanka, Solujić, Slavica, Mladenović, Milan, Stanković, Nevena, Mihailović, Vladimir, Katanić, Jelena, Mihailović, Mirjana, "Chemical composition, antioxidant and antigenotoxic activities of Cotinus coggygria stem extract" in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia (2013):90-91,
https://hdl.handle.net/21.15107/rcub_ibiss_6141 .

TY  - CONF
AU  - Mihailović, Vladimir
AU  - Katanić, Jelena
AU  - Mihailović, Mirjana
AU  - Mišić, Danijela
AU  - Solujić, Slavica
AU  - Šipovac, Katarina
AU  - Stanković, Vesna
AU  - Mladenović, Milan
AU  - Stanković, Nevena
PY  - 2013
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6143
AB  - Gentiana plants are best known for their bitter taste that is due to the secoiridoids (e.g. swertiamarin, gen­tiopicrin, sweroside and amarogentin). Secoiridoid glucosides, gentiopicrin, swertiamarin and sweroside, are present in various traditional medicine preparations and are reported to have hepatoprotective activity. Many Gentiana species are known for their pharmaceutical values, such as Gentiana cruciata L., commonly called cross gentian. The dried roots and above-ground parts of G. cruciata are consumed in the Balkan region as herbal tea or a medicinal wine for loss of appetite, as a stomachic and component in preparations showing benefi­cial effects in gall and liver diseases. This study using in vivo model investigates hepatoprotective activity of the methanol extract of G. cruciata root (GCR) against carbon tetrachloride-induced liver injury in rats. Wistar rats were orally pretreated with GCR (100, 200, and 400 mg/kg) and silymarin (100 mg/kg) for seven days be­fore they were treated with CCl4 (1 ml/kg, 1 :1 mixture in olive oil) which caused liver injury. Pretreatment with GCR dose-dependently and significantly (p < 0.001) decreased levels of serum transaminases, alkaline phos­phatase and total bilirubin, where as an increase was found in the level of total protein compared with CCl4-treated group. In the liver tissue antioxidant studies, we found a significant increase in the levels of catalase, superoxide dismutase and reduced glutathione, whereas there was marked reduction in the levels of thiobar­bituric acid-reactive substances, as compared to CC14 treated group. Histological analyses also show that GCR reduced the incidence of liver lesions including necrosis, ballooning degeneration and micro- and macrove­sicular changes induced by CC14 in rats. The main secoiridoid compounds (sweroside, swertiamarin and gen­tiopicrin) present in GCR were identified and quantified to gain an insight into the compounds responsible for its hepatoprotective effects. The HPLC assay clearly indicated that GCR contained the greatest concentra­tion of gentiopicrin (5.45%), whereas concentration of sweroside (0.29%) and swertiamarin (0.09%) were lower.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia
T1  - Secoiridoid content and hepatoprotective activity of Gentiana cruciata L. root extract
SP  - 91
EP  - 92
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6143
ER  - 

@conference{
author = "Mihailović, Vladimir and Katanić, Jelena and Mihailović, Mirjana and Mišić, Danijela and Solujić, Slavica and Šipovac, Katarina and Stanković, Vesna and Mladenović, Milan and Stanković, Nevena",
year = "2013",
abstract = "Gentiana plants are best known for their bitter taste that is due to the secoiridoids (e.g. swertiamarin, gen­tiopicrin, sweroside and amarogentin). Secoiridoid glucosides, gentiopicrin, swertiamarin and sweroside, are present in various traditional medicine preparations and are reported to have hepatoprotective activity. Many Gentiana species are known for their pharmaceutical values, such as Gentiana cruciata L., commonly called cross gentian. The dried roots and above-ground parts of G. cruciata are consumed in the Balkan region as herbal tea or a medicinal wine for loss of appetite, as a stomachic and component in preparations showing benefi­cial effects in gall and liver diseases. This study using in vivo model investigates hepatoprotective activity of the methanol extract of G. cruciata root (GCR) against carbon tetrachloride-induced liver injury in rats. Wistar rats were orally pretreated with GCR (100, 200, and 400 mg/kg) and silymarin (100 mg/kg) for seven days be­fore they were treated with CCl4 (1 ml/kg, 1 :1 mixture in olive oil) which caused liver injury. Pretreatment with GCR dose-dependently and significantly (p < 0.001) decreased levels of serum transaminases, alkaline phos­phatase and total bilirubin, where as an increase was found in the level of total protein compared with CCl4-treated group. In the liver tissue antioxidant studies, we found a significant increase in the levels of catalase, superoxide dismutase and reduced glutathione, whereas there was marked reduction in the levels of thiobar­bituric acid-reactive substances, as compared to CC14 treated group. Histological analyses also show that GCR reduced the incidence of liver lesions including necrosis, ballooning degeneration and micro- and macrove­sicular changes induced by CC14 in rats. The main secoiridoid compounds (sweroside, swertiamarin and gen­tiopicrin) present in GCR were identified and quantified to gain an insight into the compounds responsible for its hepatoprotective effects. The HPLC assay clearly indicated that GCR contained the greatest concentra­tion of gentiopicrin (5.45%), whereas concentration of sweroside (0.29%) and swertiamarin (0.09%) were lower.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia",
title = "Secoiridoid content and hepatoprotective activity of Gentiana cruciata L. root extract",
pages = "91-92",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6143"
}

Mihailović, V., Katanić, J., Mihailović, M., Mišić, D., Solujić, S., Šipovac, K., Stanković, V., Mladenović, M.,& Stanković, N.. (2013). Secoiridoid content and hepatoprotective activity of Gentiana cruciata L. root extract. in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia
Belgrade: Serbian Plant Physiology Society., 91-92.
https://hdl.handle.net/21.15107/rcub_ibiss_6143

Mihailović V, Katanić J, Mihailović M, Mišić D, Solujić S, Šipovac K, Stanković V, Mladenović M, Stanković N. Secoiridoid content and hepatoprotective activity of Gentiana cruciata L. root extract. in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia. 2013;:91-92.
https://hdl.handle.net/21.15107/rcub_ibiss_6143 .

Mihailović, Vladimir, Katanić, Jelena, Mihailović, Mirjana, Mišić, Danijela, Solujić, Slavica, Šipovac, Katarina, Stanković, Vesna, Mladenović, Milan, Stanković, Nevena, "Secoiridoid content and hepatoprotective activity of Gentiana cruciata L. root extract" in Programme and Abstracts: 1st International Conference on Plant Biology and 20th Symposium of the Serbian Plant Physiology Society; 2013 Jun 4-7; Subotica, Serbia (2013):91-92,
https://hdl.handle.net/21.15107/rcub_ibiss_6143 .