TY  - JOUR
AU  - Rashidian, Ghasem
AU  - Shahin, Khalid
AU  - Elshopakey, Gehad E.
AU  - Mahboub, Heba H.
AU  - Fahim, Azin
AU  - Elabd, Hiam
AU  - Prokić, Marko
AU  - Faggio, Caterina
PY  - 2022
UR  - https://www.mdpi.com/2077-1312/10/3/325
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4893
AB  - Medicinal plants are increasingly used in aquaculture owing to their beneficial impacts on the health status of farmed fish. The current study was conducted to investigate the effect of nutmeg (Myristica fragrans) extract on growth, immunity, antioxidant parameters, and resistance of common carp (Cyprinus carpio) against Aeromonas hydrophila. In addition, in vitro antibacterial activity of the skin mucus of fish fed on nutmeg extract was evaluated against three major fish pathogenic bacteria through the standard disk diffusion method. Fish (17.27 ± 0.11 g) were divided into four groups and fed on experimental diets containing different levels of nutmeg extract, including zero (control), 0.5% (M1), 1% (M2), and 2% (M3) per kg diet. Results showed that nutmeg significantly enhanced growth parameters after a four-week feeding trial. Feed conversion ratio was remarkably reduced with the lowest value reported for the M3 group, whereas weight gain was notably increased in M2 and M3. No significant effect was found on the hematological profile, including mean corpuscular hemoglobin concentration, mean corpuscular hemoglobin, mean corpuscular volume, and hematocrit, while the highest levels of red blood cells and white blood cells were found in the M3 group. Stress biomarkers, including glucose and cortisol, were the lowest in the M3 group. Serum and skin mucus immunological and antioxidant parameters were significantly higher in M3, followed by M2, where the highest resistance was also observed. In addition, skin mucus samples effectively inhibited Streptococcus iniae, Yersinia ruckeri, and Aeromonas hydrophila. Overall, the present results suggest that dietary nutmeg (20 g/kg diet) could be used as a growth promotor and immunostimulant in common carp.
PB  - Basel: MDPI
T2  - Journal of Marine Science and Engineering
T1  - The Dietary Effects of Nutmeg (Myristica fragrans) Extract on Growth, Hematological Parameters, Immunity, Antioxidant Status, and Disease Resistance of Common Carp (Cyprinus carpio) against Aeromonas hydrophila
IS  - 3
VL  - 10
DO  - 10.3390/jmse10030325
SP  - 325
ER  - 

@article{
author = "Rashidian, Ghasem and Shahin, Khalid and Elshopakey, Gehad E. and Mahboub, Heba H. and Fahim, Azin and Elabd, Hiam and Prokić, Marko and Faggio, Caterina",
year = "2022",
abstract = "Medicinal plants are increasingly used in aquaculture owing to their beneficial impacts on the health status of farmed fish. The current study was conducted to investigate the effect of nutmeg (Myristica fragrans) extract on growth, immunity, antioxidant parameters, and resistance of common carp (Cyprinus carpio) against Aeromonas hydrophila. In addition, in vitro antibacterial activity of the skin mucus of fish fed on nutmeg extract was evaluated against three major fish pathogenic bacteria through the standard disk diffusion method. Fish (17.27 ± 0.11 g) were divided into four groups and fed on experimental diets containing different levels of nutmeg extract, including zero (control), 0.5% (M1), 1% (M2), and 2% (M3) per kg diet. Results showed that nutmeg significantly enhanced growth parameters after a four-week feeding trial. Feed conversion ratio was remarkably reduced with the lowest value reported for the M3 group, whereas weight gain was notably increased in M2 and M3. No significant effect was found on the hematological profile, including mean corpuscular hemoglobin concentration, mean corpuscular hemoglobin, mean corpuscular volume, and hematocrit, while the highest levels of red blood cells and white blood cells were found in the M3 group. Stress biomarkers, including glucose and cortisol, were the lowest in the M3 group. Serum and skin mucus immunological and antioxidant parameters were significantly higher in M3, followed by M2, where the highest resistance was also observed. In addition, skin mucus samples effectively inhibited Streptococcus iniae, Yersinia ruckeri, and Aeromonas hydrophila. Overall, the present results suggest that dietary nutmeg (20 g/kg diet) could be used as a growth promotor and immunostimulant in common carp.",
publisher = "Basel: MDPI",
journal = "Journal of Marine Science and Engineering",
title = "The Dietary Effects of Nutmeg (Myristica fragrans) Extract on Growth, Hematological Parameters, Immunity, Antioxidant Status, and Disease Resistance of Common Carp (Cyprinus carpio) against Aeromonas hydrophila",
number = "3",
volume = "10",
doi = "10.3390/jmse10030325",
pages = "325"
}

Rashidian, G., Shahin, K., Elshopakey, G. E., Mahboub, H. H., Fahim, A., Elabd, H., Prokić, M.,& Faggio, C.. (2022). The Dietary Effects of Nutmeg (Myristica fragrans) Extract on Growth, Hematological Parameters, Immunity, Antioxidant Status, and Disease Resistance of Common Carp (Cyprinus carpio) against Aeromonas hydrophila. in Journal of Marine Science and Engineering
Basel: MDPI., 10(3), 325.
https://doi.org/10.3390/jmse10030325

Rashidian G, Shahin K, Elshopakey GE, Mahboub HH, Fahim A, Elabd H, Prokić M, Faggio C. The Dietary Effects of Nutmeg (Myristica fragrans) Extract on Growth, Hematological Parameters, Immunity, Antioxidant Status, and Disease Resistance of Common Carp (Cyprinus carpio) against Aeromonas hydrophila. in Journal of Marine Science and Engineering. 2022;10(3):325.
doi:10.3390/jmse10030325 .

Rashidian, Ghasem, Shahin, Khalid, Elshopakey, Gehad E., Mahboub, Heba H., Fahim, Azin, Elabd, Hiam, Prokić, Marko, Faggio, Caterina, "The Dietary Effects of Nutmeg (Myristica fragrans) Extract on Growth, Hematological Parameters, Immunity, Antioxidant Status, and Disease Resistance of Common Carp (Cyprinus carpio) against Aeromonas hydrophila" in Journal of Marine Science and Engineering, 10, no. 3 (2022):325,
https://doi.org/10.3390/jmse10030325 . .