TY  - JOUR
AU  - Marković, Marija
AU  - Trifunović-Momčilov, Milana
AU  - Radulović, Olga
AU  - Paunović, Danijela
AU  - Antonić Reljin, Dragana
AU  - Jevremović, Slađana
AU  - Uzelac, Branka
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6522
AB  - The genus Fritillaria is characterized by the production of alkaloids, primarily of the isosteroidal type. The aim of this preliminary study was to perform a histolocalization screening aimed at revealing the presence of alkaloids in the bulbs of F. meleagris, cultured in vitro under different growth regimes and at different developmental stages, as a powerful tool to identify preferable in vitro plant material for alkaloid isolation. Histochemical localization of alkaloids was performed using Wagner’s and Dragendorff’s reagents in fresh sections of bulbs cultured at 24 °C or 7 °C for 4 weeks, as well as those cultured at 24 °C following the 4-week chilling treatment, which were sampled at the beginning of sprouting. A positive reaction was observed with both reagents and was particularly intense in cold-treated bulbs. Alkaloids were mainly distributed in the bulbs and partly in the sprouts of the in vitro grown F. meleagris. The most intense staining, indicative of high alkaloid content, was observed in the bulb scales of pre-chilled bulbs that sprouted at 24 °C, rendering them preferable as in vitro plant material for alkaloid isolation. The results suggest that alkaloid production in the bulbs of F. meleagris can be improved by manipulating growth in the microenvironment of in vitro cultures, in order to meet the increasing industrial demand for medicinally or commercially important metabolites used as traditional medicines and herbal remedies.
PB  - Basel : MDPI
T2  - Horticulturae
T1  - Histochemical Localization of Alkaloids in the Bulbs of In Vitro-Regenerated Snake’s Head Fritillary (Fritillaria meleagris L.): The Effect of a Temperature Regime
IS  - 1
VL  - 10
DO  - 10.3390/horticulturae10010017
SP  - 17
ER  - 

@article{
author = "Marković, Marija and Trifunović-Momčilov, Milana and Radulović, Olga and Paunović, Danijela and Antonić Reljin, Dragana and Jevremović, Slađana and Uzelac, Branka",
year = "2024",
abstract = "The genus Fritillaria is characterized by the production of alkaloids, primarily of the isosteroidal type. The aim of this preliminary study was to perform a histolocalization screening aimed at revealing the presence of alkaloids in the bulbs of F. meleagris, cultured in vitro under different growth regimes and at different developmental stages, as a powerful tool to identify preferable in vitro plant material for alkaloid isolation. Histochemical localization of alkaloids was performed using Wagner’s and Dragendorff’s reagents in fresh sections of bulbs cultured at 24 °C or 7 °C for 4 weeks, as well as those cultured at 24 °C following the 4-week chilling treatment, which were sampled at the beginning of sprouting. A positive reaction was observed with both reagents and was particularly intense in cold-treated bulbs. Alkaloids were mainly distributed in the bulbs and partly in the sprouts of the in vitro grown F. meleagris. The most intense staining, indicative of high alkaloid content, was observed in the bulb scales of pre-chilled bulbs that sprouted at 24 °C, rendering them preferable as in vitro plant material for alkaloid isolation. The results suggest that alkaloid production in the bulbs of F. meleagris can be improved by manipulating growth in the microenvironment of in vitro cultures, in order to meet the increasing industrial demand for medicinally or commercially important metabolites used as traditional medicines and herbal remedies.",
publisher = "Basel : MDPI",
journal = "Horticulturae",
title = "Histochemical Localization of Alkaloids in the Bulbs of In Vitro-Regenerated Snake’s Head Fritillary (Fritillaria meleagris L.): The Effect of a Temperature Regime",
number = "1",
volume = "10",
doi = "10.3390/horticulturae10010017",
pages = "17"
}

Marković, M., Trifunović-Momčilov, M., Radulović, O., Paunović, D., Antonić Reljin, D., Jevremović, S.,& Uzelac, B.. (2024). Histochemical Localization of Alkaloids in the Bulbs of In Vitro-Regenerated Snake’s Head Fritillary (Fritillaria meleagris L.): The Effect of a Temperature Regime. in Horticulturae
Basel : MDPI., 10(1), 17.
https://doi.org/10.3390/horticulturae10010017

Marković M, Trifunović-Momčilov M, Radulović O, Paunović D, Antonić Reljin D, Jevremović S, Uzelac B. Histochemical Localization of Alkaloids in the Bulbs of In Vitro-Regenerated Snake’s Head Fritillary (Fritillaria meleagris L.): The Effect of a Temperature Regime. in Horticulturae. 2024;10(1):17.
doi:10.3390/horticulturae10010017 .

Marković, Marija, Trifunović-Momčilov, Milana, Radulović, Olga, Paunović, Danijela, Antonić Reljin, Dragana, Jevremović, Slađana, Uzelac, Branka, "Histochemical Localization of Alkaloids in the Bulbs of In Vitro-Regenerated Snake’s Head Fritillary (Fritillaria meleagris L.): The Effect of a Temperature Regime" in Horticulturae, 10, no. 1 (2024):17,
https://doi.org/10.3390/horticulturae10010017 . .

TY  - JOUR
AU  - Marković, Marija
AU  - Trifunović-Momčilov, Milana
AU  - Radulović, Olga
AU  - Paunović, Danijela
AU  - Antonić Reljin, Dragana
AU  - Uzelac, Branka
AU  - Subotić, Angelina
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6089
AB  - Fritillaria meleagris is a horticulturally and medicinally valuable bulbous plant that requires a period of low temperatures for proper growth and flowering. Since conventional methods of propagation are ineffective and very slow, tissue culture techniques offer an integrated approach to mass production of this valuable geophyte. In this study, we investigated the effects of various auxin–cytokinin combinations on different morphogenetic pathways in bulb scale culture. Bulbs obtained in vitro were cut longitudinally, and bulb scales were cultured for four weeks at 7 °C on MS medium supplemented with 6-benzylaminopurine (BAP) in combination with 2,4-dichlorophenoxyacetic acid (2,4-D) or α-naphthaleneacetic acid (NAA) at different concentrations in order to investigate the influence of plant growth regulators (PGRs) on different morphogenetic responses. Regeneration percentage, number of shoots per explant, shoot length, number of bulbs and number of somatic embryos were monitored weekly. After chilling, bulb scales were transferred to 24 °C, and all parameters were recorded again. Low PGR concentrations were very effective for shoot multiplication, yielding up to 5.5 shoots per explant. 2,4-D (at 2 mg/L) in combination with low BAP (0.25 mg/L) produced the highest number of bulbs (11.00 ± 0.00), while PGR-free medium was extremely effective in somatic embryo formation (13.50 ± 2.90). Detached somatic embryos and bulblets continued to grow and develop on fresh PGR-free medium. We present data demonstrating that low auxin–cytokinin concentrations and PGR-free medium provide an effective method for a combined morphogenetic pathway in F. meleagris that is suitable for large-scale propagation.
PB  - Basel : MDPI
T2  - Horticulturae
T1  - The Effects of Different Auxin–Cytokinin Combinations on Morphogenesis of Fritillaria meleagris Using Bulb Scale Sections In Vitro
IS  - 8
VL  - 9
DO  - 10.3390/horticulturae9080910
SP  - 910
ER  - 

@article{
author = "Marković, Marija and Trifunović-Momčilov, Milana and Radulović, Olga and Paunović, Danijela and Antonić Reljin, Dragana and Uzelac, Branka and Subotić, Angelina",
year = "2023",
abstract = "Fritillaria meleagris is a horticulturally and medicinally valuable bulbous plant that requires a period of low temperatures for proper growth and flowering. Since conventional methods of propagation are ineffective and very slow, tissue culture techniques offer an integrated approach to mass production of this valuable geophyte. In this study, we investigated the effects of various auxin–cytokinin combinations on different morphogenetic pathways in bulb scale culture. Bulbs obtained in vitro were cut longitudinally, and bulb scales were cultured for four weeks at 7 °C on MS medium supplemented with 6-benzylaminopurine (BAP) in combination with 2,4-dichlorophenoxyacetic acid (2,4-D) or α-naphthaleneacetic acid (NAA) at different concentrations in order to investigate the influence of plant growth regulators (PGRs) on different morphogenetic responses. Regeneration percentage, number of shoots per explant, shoot length, number of bulbs and number of somatic embryos were monitored weekly. After chilling, bulb scales were transferred to 24 °C, and all parameters were recorded again. Low PGR concentrations were very effective for shoot multiplication, yielding up to 5.5 shoots per explant. 2,4-D (at 2 mg/L) in combination with low BAP (0.25 mg/L) produced the highest number of bulbs (11.00 ± 0.00), while PGR-free medium was extremely effective in somatic embryo formation (13.50 ± 2.90). Detached somatic embryos and bulblets continued to grow and develop on fresh PGR-free medium. We present data demonstrating that low auxin–cytokinin concentrations and PGR-free medium provide an effective method for a combined morphogenetic pathway in F. meleagris that is suitable for large-scale propagation.",
publisher = "Basel : MDPI",
journal = "Horticulturae",
title = "The Effects of Different Auxin–Cytokinin Combinations on Morphogenesis of Fritillaria meleagris Using Bulb Scale Sections In Vitro",
number = "8",
volume = "9",
doi = "10.3390/horticulturae9080910",
pages = "910"
}

Marković, M., Trifunović-Momčilov, M., Radulović, O., Paunović, D., Antonić Reljin, D., Uzelac, B.,& Subotić, A.. (2023). The Effects of Different Auxin–Cytokinin Combinations on Morphogenesis of Fritillaria meleagris Using Bulb Scale Sections In Vitro. in Horticulturae
Basel : MDPI., 9(8), 910.
https://doi.org/10.3390/horticulturae9080910

Marković M, Trifunović-Momčilov M, Radulović O, Paunović D, Antonić Reljin D, Uzelac B, Subotić A. The Effects of Different Auxin–Cytokinin Combinations on Morphogenesis of Fritillaria meleagris Using Bulb Scale Sections In Vitro. in Horticulturae. 2023;9(8):910.
doi:10.3390/horticulturae9080910 .

Marković, Marija, Trifunović-Momčilov, Milana, Radulović, Olga, Paunović, Danijela, Antonić Reljin, Dragana, Uzelac, Branka, Subotić, Angelina, "The Effects of Different Auxin–Cytokinin Combinations on Morphogenesis of Fritillaria meleagris Using Bulb Scale Sections In Vitro" in Horticulturae, 9, no. 8 (2023):910,
https://doi.org/10.3390/horticulturae9080910 . .

TY  - THES
AU  - Antonić Reljin, Dragana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4972
AB  - U prvom delu doktorske disertacije ispitivan je odgovor biljaka Impatiens walleriana na vodni stres indukovan polietilen glikolom (PEG) i uticaj salicilne kiseline (SA) u in vitro uslovima. Kulture izdanaka I. walleriana su gajene na hranljivoj podlozi koja je sadržala različite kombinacije i koncentracije PEG (1-3%) i SA (1-3 mM). Nakon dugotrajnog delovanja vodnog stresa na biljke I. walleriana utvrđen je uticaj PEG i SA na parametre hidratacije, indikatore oksidativnog stresa, kao i na parametre rastenja izdanaka. PEG indukovani vodni stres je uzrokovao redukciju rastenja, smanjenje mase svežih izdanaka, broja listova i izdanaka, relativnog sadržaja vode (RWC) i sadržaja hlorofila, kao i akumulaciju prolina, H2O2, i malondialdehida (MDA). Aktivnosti antioksidativnih enzima, katalaza (CAT), superoksid dismutaza (SOD) i peroksidaza (POX), su povećane kao odgovor na PEG tretman i to zavisno od primenjene koncentracije PEG. U drugom delu doktorske disertacije ispitivan je uticaj folijarne primene 2 mM SA na I. walleriana gajene ex vitro u uslovima suše. Biljke su podeljene u 4 grupe: zalivane biljke, zalivane biljke tretirane sa SA, nezalivane biljke i nezalivane biljke tretirane sa SA. Utvrđen je broj cvetnih pupoljaka i cvetova, RWC, sadržaj MDA, prolina, kao i aktivnosti SOD, CAT i POX u različitim vremenskim tačkama tokom trajanja eksperimenta. Suša je dovela do venjenja, odbacivanja cvetova, smanjenja RWC i porasta MDA. Kao odgovor na sušu kod I. walleraina je pojačana aktivnost hloroplastne Cu/ZnSOD i dve izoforme gvajakol peroksidaze. Identifikovane su tri de novo sekvence dehidrina korišćenjem transkriptoma iz listova I. walleriana: IwDhn1, IwDhn2.1 i IwDhn2.2. Suša je indukovala ekspresiju IwDhn2.1 i IwDhn2.2. Primena SA je pozitivno uticala na biljke koje su bile izložene suši, sprečavajući venjenje, održavajući RWC i aktivnost antioksidativnih enzima, ali nije imala uticaj na sprečavanje odbacivanja cvetova.
AB  - In the first part of dissertation the responses of Impatiens walleriana to polyethylene glycol (PEG) induced water stress and the potential of exogenous salicylic acid (SA) as stress-ameliorating agent was described. Impatiens shoot culture was established on medium containing different concentrations and combinations of PEG (1–3% ) and SA (1–3 mM). After prolonged drought, water relation parameters, oxidative stress indicators, and growth responses of the shoots to PEG and/or SA were recorded. PEG reduced growth, fresh weight of shoots, the number of developed leaves and shoots, relative water content (RWC), and chlorophyll content. PEG caused accumulation of proline, H2O2, and malondialdehyde (MDA). The activities of catalase (CAT), superoxide dismutase (SOD), and peroxidase (POX) were increased in response to PEG in a dose-dependent manner. In the second part of dissertation it was investigated whether the foliar application of 2 mM SA could protect ex vitro grown I. walleriana plants from drought stress. The plants were divided into 4 groups: watered plants, drought-stressed plants, watered plants treated with SA and drought-stressed plants treated with SA. The number of flower buds and flowers, RWC, contents of MDA, proline and the activities of superoxide dismutases, catalases and peroxidases were recorded at different time points. Three dehydrin sequences were identified in de novo assembled leaf transcriptome: IwDhn1, IwDhn2.1 and IwDhn2.2. Drought stress caused wilting, floral abortion, reduction of RWC and increased MDA. In response to drought, I. walleriana induced chloroplastic Cu/ZnSOD and two guaiacol peroxidase isoforms. The remarkable drought response was induction of IwDhn2.1 and IwDhn2.2. SA had ameliorating effects on plants exposed to drought, including prevention of wilting, preservation of RWC, modulation of antioxidative activities, but had no effects on flowers preservation.
PB  - Belgrade: Faculty of Biology, University of Belgrade
T2  - Faculty of Biology, University of Belgrade
T1  - Uticaj salicilne kiseline na biohemijske mehanizme tolerancije Impatiens walleriana (Balsaminaceae) prema vodnom stresu
T1  - Effect of salycilic acid on biochemical tolerance mechanisams of Impatiens walleriana (Balsaminaceae) to water stress
SP  - 1
EP  - 119
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4972
ER  - 

@phdthesis{
author = "Antonić Reljin, Dragana",
year = "2022",
abstract = "U prvom delu doktorske disertacije ispitivan je odgovor biljaka Impatiens walleriana na vodni stres indukovan polietilen glikolom (PEG) i uticaj salicilne kiseline (SA) u in vitro uslovima. Kulture izdanaka I. walleriana su gajene na hranljivoj podlozi koja je sadržala različite kombinacije i koncentracije PEG (1-3%) i SA (1-3 mM). Nakon dugotrajnog delovanja vodnog stresa na biljke I. walleriana utvrđen je uticaj PEG i SA na parametre hidratacije, indikatore oksidativnog stresa, kao i na parametre rastenja izdanaka. PEG indukovani vodni stres je uzrokovao redukciju rastenja, smanjenje mase svežih izdanaka, broja listova i izdanaka, relativnog sadržaja vode (RWC) i sadržaja hlorofila, kao i akumulaciju prolina, H2O2, i malondialdehida (MDA). Aktivnosti antioksidativnih enzima, katalaza (CAT), superoksid dismutaza (SOD) i peroksidaza (POX), su povećane kao odgovor na PEG tretman i to zavisno od primenjene koncentracije PEG. U drugom delu doktorske disertacije ispitivan je uticaj folijarne primene 2 mM SA na I. walleriana gajene ex vitro u uslovima suše. Biljke su podeljene u 4 grupe: zalivane biljke, zalivane biljke tretirane sa SA, nezalivane biljke i nezalivane biljke tretirane sa SA. Utvrđen je broj cvetnih pupoljaka i cvetova, RWC, sadržaj MDA, prolina, kao i aktivnosti SOD, CAT i POX u različitim vremenskim tačkama tokom trajanja eksperimenta. Suša je dovela do venjenja, odbacivanja cvetova, smanjenja RWC i porasta MDA. Kao odgovor na sušu kod I. walleraina je pojačana aktivnost hloroplastne Cu/ZnSOD i dve izoforme gvajakol peroksidaze. Identifikovane su tri de novo sekvence dehidrina korišćenjem transkriptoma iz listova I. walleriana: IwDhn1, IwDhn2.1 i IwDhn2.2. Suša je indukovala ekspresiju IwDhn2.1 i IwDhn2.2. Primena SA je pozitivno uticala na biljke koje su bile izložene suši, sprečavajući venjenje, održavajući RWC i aktivnost antioksidativnih enzima, ali nije imala uticaj na sprečavanje odbacivanja cvetova., In the first part of dissertation the responses of Impatiens walleriana to polyethylene glycol (PEG) induced water stress and the potential of exogenous salicylic acid (SA) as stress-ameliorating agent was described. Impatiens shoot culture was established on medium containing different concentrations and combinations of PEG (1–3% ) and SA (1–3 mM). After prolonged drought, water relation parameters, oxidative stress indicators, and growth responses of the shoots to PEG and/or SA were recorded. PEG reduced growth, fresh weight of shoots, the number of developed leaves and shoots, relative water content (RWC), and chlorophyll content. PEG caused accumulation of proline, H2O2, and malondialdehyde (MDA). The activities of catalase (CAT), superoxide dismutase (SOD), and peroxidase (POX) were increased in response to PEG in a dose-dependent manner. In the second part of dissertation it was investigated whether the foliar application of 2 mM SA could protect ex vitro grown I. walleriana plants from drought stress. The plants were divided into 4 groups: watered plants, drought-stressed plants, watered plants treated with SA and drought-stressed plants treated with SA. The number of flower buds and flowers, RWC, contents of MDA, proline and the activities of superoxide dismutases, catalases and peroxidases were recorded at different time points. Three dehydrin sequences were identified in de novo assembled leaf transcriptome: IwDhn1, IwDhn2.1 and IwDhn2.2. Drought stress caused wilting, floral abortion, reduction of RWC and increased MDA. In response to drought, I. walleriana induced chloroplastic Cu/ZnSOD and two guaiacol peroxidase isoforms. The remarkable drought response was induction of IwDhn2.1 and IwDhn2.2. SA had ameliorating effects on plants exposed to drought, including prevention of wilting, preservation of RWC, modulation of antioxidative activities, but had no effects on flowers preservation.",
publisher = "Belgrade: Faculty of Biology, University of Belgrade",
journal = "Faculty of Biology, University of Belgrade",
title = "Uticaj salicilne kiseline na biohemijske mehanizme tolerancije Impatiens walleriana (Balsaminaceae) prema vodnom stresu, Effect of salycilic acid on biochemical tolerance mechanisams of Impatiens walleriana (Balsaminaceae) to water stress",
pages = "1-119",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4972"
}

Antonić Reljin, D.. (2022). Uticaj salicilne kiseline na biohemijske mehanizme tolerancije Impatiens walleriana (Balsaminaceae) prema vodnom stresu. in Faculty of Biology, University of Belgrade
Belgrade: Faculty of Biology, University of Belgrade., 1-119.
https://hdl.handle.net/21.15107/rcub_ibiss_4972

Antonić Reljin D. Uticaj salicilne kiseline na biohemijske mehanizme tolerancije Impatiens walleriana (Balsaminaceae) prema vodnom stresu. in Faculty of Biology, University of Belgrade. 2022;:1-119.
https://hdl.handle.net/21.15107/rcub_ibiss_4972 .

Antonić Reljin, Dragana, "Uticaj salicilne kiseline na biohemijske mehanizme tolerancije Impatiens walleriana (Balsaminaceae) prema vodnom stresu" in Faculty of Biology, University of Belgrade (2022):1-119,
https://hdl.handle.net/21.15107/rcub_ibiss_4972 .

TY  - JOUR
AU  - Antonić Reljin, Dragana
AU  - Subotić, Angelina
AU  - Dragićević, Milan
AU  - Pantelić, Danijel
AU  - Milošević, Snežana
AU  - Simonović, Ana
AU  - Momčilović, Ivana
PY  - 2020
UR  - https://www.mdpi.com/2223-7747/9/11/1589
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4009
AB  - Impatiens walleriana is a valued ornamental plant sensitive to drought stress. We investigated whether the foliar application of 2mM salicylic acid (SA) can protect potted I. walleriana plants from drought stress. The plants were divided into: watered plants, drought-stressed plants, watered plants treated with SA and drought-stressed plants treated with SA. The number of flowers and flower buds, relative water content (RWC), contents of malondialdehyde (MDA) and proline (Pro) and the activities of superoxide dismutases, catalases and peroxidases were recorded at different time points. Three dehydrin sequences were identified in de novo assembled leaf transcriptome: IwDhn1, IwDhn2.1 and IwDhn2.2. Drought stress caused wilting, floral abortion, reduction of RWC and increased MDA—an indicator of lipid peroxidation. In response to drought, Impatiens accumulated Pro and induced chloroplastic Cu/ZnSOD and two peroxidase isoforms. The most remarkable drought response was strong induction of IwDhn2.1 and IwDhn2.2. Rehydration restored RWC, Pro level, Cu/ZnSOD activity and dehydrins expression in drought-stressed plants approximately to the values of watered plants.SA had ameliorating effects on plants exposed to drought, including prevention of wilting, preservation of RWC, increased Pro accumulation, modulation of antioxidative activities and remarkable decrease of lipid peroxidation, but without effects on flowers’ preservation.
PB  - MDPI AG
T2  - Plants
T1  - Effects of Exogenous Salicylic Acid on Drought Response and Characterization of Dehydrins in Impatiens walleriana
IS  - 11
VL  - 9
DO  - 10.3390/plants9111589
SP  - 1589
ER  - 

@article{
author = "Antonić Reljin, Dragana and Subotić, Angelina and Dragićević, Milan and Pantelić, Danijel and Milošević, Snežana and Simonović, Ana and Momčilović, Ivana",
year = "2020",
abstract = "Impatiens walleriana is a valued ornamental plant sensitive to drought stress. We investigated whether the foliar application of 2mM salicylic acid (SA) can protect potted I. walleriana plants from drought stress. The plants were divided into: watered plants, drought-stressed plants, watered plants treated with SA and drought-stressed plants treated with SA. The number of flowers and flower buds, relative water content (RWC), contents of malondialdehyde (MDA) and proline (Pro) and the activities of superoxide dismutases, catalases and peroxidases were recorded at different time points. Three dehydrin sequences were identified in de novo assembled leaf transcriptome: IwDhn1, IwDhn2.1 and IwDhn2.2. Drought stress caused wilting, floral abortion, reduction of RWC and increased MDA—an indicator of lipid peroxidation. In response to drought, Impatiens accumulated Pro and induced chloroplastic Cu/ZnSOD and two peroxidase isoforms. The most remarkable drought response was strong induction of IwDhn2.1 and IwDhn2.2. Rehydration restored RWC, Pro level, Cu/ZnSOD activity and dehydrins expression in drought-stressed plants approximately to the values of watered plants.SA had ameliorating effects on plants exposed to drought, including prevention of wilting, preservation of RWC, increased Pro accumulation, modulation of antioxidative activities and remarkable decrease of lipid peroxidation, but without effects on flowers’ preservation.",
publisher = "MDPI AG",
journal = "Plants",
title = "Effects of Exogenous Salicylic Acid on Drought Response and Characterization of Dehydrins in Impatiens walleriana",
number = "11",
volume = "9",
doi = "10.3390/plants9111589",
pages = "1589"
}

Antonić Reljin, D., Subotić, A., Dragićević, M., Pantelić, D., Milošević, S., Simonović, A.,& Momčilović, I.. (2020). Effects of Exogenous Salicylic Acid on Drought Response and Characterization of Dehydrins in Impatiens walleriana. in Plants
MDPI AG., 9(11), 1589.
https://doi.org/10.3390/plants9111589

Antonić Reljin D, Subotić A, Dragićević M, Pantelić D, Milošević S, Simonović A, Momčilović I. Effects of Exogenous Salicylic Acid on Drought Response and Characterization of Dehydrins in Impatiens walleriana. in Plants. 2020;9(11):1589.
doi:10.3390/plants9111589 .

Antonić Reljin, Dragana, Subotić, Angelina, Dragićević, Milan, Pantelić, Danijel, Milošević, Snežana, Simonović, Ana, Momčilović, Ivana, "Effects of Exogenous Salicylic Acid on Drought Response and Characterization of Dehydrins in Impatiens walleriana" in Plants, 9, no. 11 (2020):1589,
https://doi.org/10.3390/plants9111589 . .

TY  - JOUR
AU  - Trajković, Milena
AU  - Antonić Reljin, Dragana
AU  - Cingel, Aleksandar
AU  - Ghalawenji, Nabil
AU  - Subotić, Angelina
AU  - Jevremović, Slađana
PY  - 2019
UR  - http://link.springer.com/10.1007/s13205-018-1540-4
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC6314937
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3232
AB  - The aim of this study was to develop a fast, reliable and true-to-type protocol for in vitro plant regeneration and long-term storage of horned pansy (Viola cornuta L). Seed germination over 60% was recorded after 12 weeks of growth at 10 °C or 4 °C. Calli formation and shoot induction were obtained in petiole and hypocotyl culture on half-strength MS mineral salts with full concentration of Na-FeEDTA and vitamins (½MS medium) with 2,4-dichlorophenoxyacetic acid (2,4-D, 0.1 mg/L) and 6-benzylaminopurine (BAP, 2.0 mg/L) and leaf culture on ½MS medium with thidiazuron (TDZ,1.0 mg/L). The highest frequency of adventitious shoot induction (50%) with six shoots/explant was achieved in hypocotyl culture from top hypocotyl segments, close to epicotyl which was grown 8 weeks at 16 h light/8 h dark photoperiod. Subsequent shoot multiplication was achieved on ½MS medium with α-naphthaleneacetic acid (NAA, 0.1 or 0.5 mg/L) and BAP (1.0 mg/L). Rooting of shoots was obtained on ½MS medium with low concentration (0.1 mg/L) of auxins: indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) or NAA, or without growth regulators. In vitro-derived plantlets were acclimatized under greenhouse conditions. All plants developed normally, bloomed and set seeds. Shoot tips were cryopreserved succssefully using modified plant vitrification 3 (PVS3-based vitrification procedure). Cold acclimation for 2 weeks significantly improved shoot regrowth (64%) after thawing in comparison to non-acclimated shoots (39%). Clonal fidelity of regenerated plantlets at ploidy level was confirmed by chromosome counting. The presented protocol can be useful for mass propagation, genetic transformation studies and long-term storage of valuable Viola spp.
T2  - 3 Biotech
T1  - Advancement in protocol for in vitro seed germination, plant regeneration and cryopreservation of Viola cornuta.
IS  - 1
VL  - 9
DO  - 10.1007/s13205-018-1540-4
SP  - 17
ER  - 

@article{
author = "Trajković, Milena and Antonić Reljin, Dragana and Cingel, Aleksandar and Ghalawenji, Nabil and Subotić, Angelina and Jevremović, Slađana",
year = "2019",
abstract = "The aim of this study was to develop a fast, reliable and true-to-type protocol for in vitro plant regeneration and long-term storage of horned pansy (Viola cornuta L). Seed germination over 60% was recorded after 12 weeks of growth at 10 °C or 4 °C. Calli formation and shoot induction were obtained in petiole and hypocotyl culture on half-strength MS mineral salts with full concentration of Na-FeEDTA and vitamins (½MS medium) with 2,4-dichlorophenoxyacetic acid (2,4-D, 0.1 mg/L) and 6-benzylaminopurine (BAP, 2.0 mg/L) and leaf culture on ½MS medium with thidiazuron (TDZ,1.0 mg/L). The highest frequency of adventitious shoot induction (50%) with six shoots/explant was achieved in hypocotyl culture from top hypocotyl segments, close to epicotyl which was grown 8 weeks at 16 h light/8 h dark photoperiod. Subsequent shoot multiplication was achieved on ½MS medium with α-naphthaleneacetic acid (NAA, 0.1 or 0.5 mg/L) and BAP (1.0 mg/L). Rooting of shoots was obtained on ½MS medium with low concentration (0.1 mg/L) of auxins: indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) or NAA, or without growth regulators. In vitro-derived plantlets were acclimatized under greenhouse conditions. All plants developed normally, bloomed and set seeds. Shoot tips were cryopreserved succssefully using modified plant vitrification 3 (PVS3-based vitrification procedure). Cold acclimation for 2 weeks significantly improved shoot regrowth (64%) after thawing in comparison to non-acclimated shoots (39%). Clonal fidelity of regenerated plantlets at ploidy level was confirmed by chromosome counting. The presented protocol can be useful for mass propagation, genetic transformation studies and long-term storage of valuable Viola spp.",
journal = "3 Biotech",
title = "Advancement in protocol for in vitro seed germination, plant regeneration and cryopreservation of Viola cornuta.",
number = "1",
volume = "9",
doi = "10.1007/s13205-018-1540-4",
pages = "17"
}

Trajković, M., Antonić Reljin, D., Cingel, A., Ghalawenji, N., Subotić, A.,& Jevremović, S.. (2019). Advancement in protocol for in vitro seed germination, plant regeneration and cryopreservation of Viola cornuta.. in 3 Biotech, 9(1), 17.
https://doi.org/10.1007/s13205-018-1540-4

Trajković M, Antonić Reljin D, Cingel A, Ghalawenji N, Subotić A, Jevremović S. Advancement in protocol for in vitro seed germination, plant regeneration and cryopreservation of Viola cornuta.. in 3 Biotech. 2019;9(1):17.
doi:10.1007/s13205-018-1540-4 .

Trajković, Milena, Antonić Reljin, Dragana, Cingel, Aleksandar, Ghalawenji, Nabil, Subotić, Angelina, Jevremović, Slađana, "Advancement in protocol for in vitro seed germination, plant regeneration and cryopreservation of Viola cornuta." in 3 Biotech, 9, no. 1 (2019):17,
https://doi.org/10.1007/s13205-018-1540-4 . .

TY  - CONF
AU  - Trajković, Milena
AU  - Antonić Reljin, Dragana
AU  - Trailović, Maja
AU  - Trifunović-Momčilov, Milana
AU  - Subotić, Angelina
AU  - Jevremović, Slađana
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6722
AB  - Cryopreservation represents a suitable method for long term storage of different plant genetic
resources. The aim of this study was to develop protocol for cryopreservation of Viola cornuta
shoot tips using one step freezing method with chemical dehydration of tissue with modified
Plant Vitrification Solutions (PVS2 or PVS3). Shoot tips (1-2 mm) of two-week cold acclimated
shoots were cultured on ½MS medium with 0.3 M sucrose for one day before treatment with loading
solution (2 M glycerol, 0.4 M sucrose) for 30 min. Osmotic dehydration with PVS2 solution (30% glycerol, 15% ethylene glycol and 15% DMSO in liquid ½MS medium with 0.4 M sucrose) were
tested at 0 °C or 24 °C. Osmotic dehydration with PVS3 (50% sucrose, 50% glycerol in liquid ½MS
medium) were tested at 24 °C for 45 min. After the treatment the explants were directly immersed
in liquid nitrogen (LN) for at least one day. Re-warming was performed at 42 °C in water bath
for 2 min. After re-warming, the PVS solutions were replaced with unloading solution containing
1.2 M sucrose for 20 min. Re-warmed shoot tips were cultured on ½MS medium with 0.1 mg L-1
BAP. We observed that PVS2 solution is cytotoxic for V. cornuta shoot tips and cannot be used for
cryopreservation. However, cryopreservation with PVS3 solution was successful, where 71.9-100%
shoot tips survived treatment before immersion to LN and 31-40% survived after re-warming from
LN. Regrowth of cryopreserved shoot tips with new well-formed leaves was obtained after four
weeks of culture.
PB  - Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade, Faculty of Biology, University of Belgrade
C3  - Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
T1  - Cryopreservation of Viola cornuta shoot tips using vitrification procedure
SP  - 91
EP  - 92
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6722
ER  - 

@conference{
author = "Trajković, Milena and Antonić Reljin, Dragana and Trailović, Maja and Trifunović-Momčilov, Milana and Subotić, Angelina and Jevremović, Slađana",
year = "2018",
abstract = "Cryopreservation represents a suitable method for long term storage of different plant genetic
resources. The aim of this study was to develop protocol for cryopreservation of Viola cornuta
shoot tips using one step freezing method with chemical dehydration of tissue with modified
Plant Vitrification Solutions (PVS2 or PVS3). Shoot tips (1-2 mm) of two-week cold acclimated
shoots were cultured on ½MS medium with 0.3 M sucrose for one day before treatment with loading
solution (2 M glycerol, 0.4 M sucrose) for 30 min. Osmotic dehydration with PVS2 solution (30% glycerol, 15% ethylene glycol and 15% DMSO in liquid ½MS medium with 0.4 M sucrose) were
tested at 0 °C or 24 °C. Osmotic dehydration with PVS3 (50% sucrose, 50% glycerol in liquid ½MS
medium) were tested at 24 °C for 45 min. After the treatment the explants were directly immersed
in liquid nitrogen (LN) for at least one day. Re-warming was performed at 42 °C in water bath
for 2 min. After re-warming, the PVS solutions were replaced with unloading solution containing
1.2 M sucrose for 20 min. Re-warmed shoot tips were cultured on ½MS medium with 0.1 mg L-1
BAP. We observed that PVS2 solution is cytotoxic for V. cornuta shoot tips and cannot be used for
cryopreservation. However, cryopreservation with PVS3 solution was successful, where 71.9-100%
shoot tips survived treatment before immersion to LN and 31-40% survived after re-warming from
LN. Regrowth of cryopreserved shoot tips with new well-formed leaves was obtained after four
weeks of culture.",
publisher = "Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade, Faculty of Biology, University of Belgrade",
journal = "Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia",
title = "Cryopreservation of Viola cornuta shoot tips using vitrification procedure",
pages = "91-92",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6722"
}

Trajković, M., Antonić Reljin, D., Trailović, M., Trifunović-Momčilov, M., Subotić, A.,& Jevremović, S.. (2018). Cryopreservation of Viola cornuta shoot tips using vitrification procedure. in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade, Faculty of Biology, University of Belgrade., 91-92.
https://hdl.handle.net/21.15107/rcub_ibiss_6722

Trajković M, Antonić Reljin D, Trailović M, Trifunović-Momčilov M, Subotić A, Jevremović S. Cryopreservation of Viola cornuta shoot tips using vitrification procedure. in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. 2018;:91-92.
https://hdl.handle.net/21.15107/rcub_ibiss_6722 .

Trajković, Milena, Antonić Reljin, Dragana, Trailović, Maja, Trifunović-Momčilov, Milana, Subotić, Angelina, Jevremović, Slađana, "Cryopreservation of Viola cornuta shoot tips using vitrification procedure" in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia (2018):91-92,
https://hdl.handle.net/21.15107/rcub_ibiss_6722 .

TY  - CONF
AU  - Trajković, Milena
AU  - Jeknić, Zoran
AU  - Antonić Reljin, Dragana
AU  - Subotić, Angelina
AU  - Jevremović, Slađana
AU  - Cingel, Aleksandar
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6540
AB  - Viola cornuta L. ‘Lutea Splendens’ is a perennial ornamental plant with small yellow flowers that
naturally grows in the Pyrenees in Spain and France. To develop novel cultivars with orange and
red flower colors, we performed Agrobacterium tumefaciens (LBA4404)-mediated transformation
with the binary vector pWBVec10a/P35S::Llccs::TNos that harbored capsanthin-capsorubin synthase
(Llccs) gene from Lilium lancifolium under the control of CaMV35S constitutive promoter and
the nopaline synthase (Nos) terminator. Capsanthin-capsorubin synthase catalyzes the conversion
of anteraxanthin and violaxanthin, two yellow ubiquitous 5-6-epoxy-xanthophylls, into capsanthin
and capsorubin, two red xanthophylls, respectively. Starting with hypocotyl explants, we developed
a transformation protocol with 0.3% shoot regeneration efficiency. Histochemical assay for
β-glucuronidase (GUS) activity showed uidA reporter gene expression in all putative Llccs-transgenic
shoots. The presence of Llccs transgene, hygromycin phosphotransferase (hpt) selectable
marker gene and uidA (GUS) reporter gene in all putative Llccs-transgenic lines were confirmed
by PCR analysis. This is the first report on Agrobacterium-mediated genetic transformation of V.
cornuta L. with the aim to introduce desirable traits into this species.
PB  - Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade Faculty of Biology, University of Belgrade
C3  - Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
T1  - Agrobacterium-mediated genetic transformation of Viola cornuta L. "Lutea Splendens" with capsanthin-capsorubin synthase gene
SP  - 152
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6540
ER  - 

@conference{
author = "Trajković, Milena and Jeknić, Zoran and Antonić Reljin, Dragana and Subotić, Angelina and Jevremović, Slađana and Cingel, Aleksandar",
year = "2018",
abstract = "Viola cornuta L. ‘Lutea Splendens’ is a perennial ornamental plant with small yellow flowers that
naturally grows in the Pyrenees in Spain and France. To develop novel cultivars with orange and
red flower colors, we performed Agrobacterium tumefaciens (LBA4404)-mediated transformation
with the binary vector pWBVec10a/P35S::Llccs::TNos that harbored capsanthin-capsorubin synthase
(Llccs) gene from Lilium lancifolium under the control of CaMV35S constitutive promoter and
the nopaline synthase (Nos) terminator. Capsanthin-capsorubin synthase catalyzes the conversion
of anteraxanthin and violaxanthin, two yellow ubiquitous 5-6-epoxy-xanthophylls, into capsanthin
and capsorubin, two red xanthophylls, respectively. Starting with hypocotyl explants, we developed
a transformation protocol with 0.3% shoot regeneration efficiency. Histochemical assay for
β-glucuronidase (GUS) activity showed uidA reporter gene expression in all putative Llccs-transgenic
shoots. The presence of Llccs transgene, hygromycin phosphotransferase (hpt) selectable
marker gene and uidA (GUS) reporter gene in all putative Llccs-transgenic lines were confirmed
by PCR analysis. This is the first report on Agrobacterium-mediated genetic transformation of V.
cornuta L. with the aim to introduce desirable traits into this species.",
publisher = "Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade Faculty of Biology, University of Belgrade",
journal = "Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia",
title = "Agrobacterium-mediated genetic transformation of Viola cornuta L. "Lutea Splendens" with capsanthin-capsorubin synthase gene",
pages = "152",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6540"
}

Trajković, M., Jeknić, Z., Antonić Reljin, D., Subotić, A., Jevremović, S.,& Cingel, A.. (2018). Agrobacterium-mediated genetic transformation of Viola cornuta L. "Lutea Splendens" with capsanthin-capsorubin synthase gene. in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade Faculty of Biology, University of Belgrade., 152.
https://hdl.handle.net/21.15107/rcub_ibiss_6540

Trajković M, Jeknić Z, Antonić Reljin D, Subotić A, Jevremović S, Cingel A. Agrobacterium-mediated genetic transformation of Viola cornuta L. "Lutea Splendens" with capsanthin-capsorubin synthase gene. in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. 2018;:152.
https://hdl.handle.net/21.15107/rcub_ibiss_6540 .

Trajković, Milena, Jeknić, Zoran, Antonić Reljin, Dragana, Subotić, Angelina, Jevremović, Slađana, Cingel, Aleksandar, "Agrobacterium-mediated genetic transformation of Viola cornuta L. "Lutea Splendens" with capsanthin-capsorubin synthase gene" in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia (2018):152,
https://hdl.handle.net/21.15107/rcub_ibiss_6540 .

TY  - CONF
AU  - Antonić Reljin, Dragana
AU  - Trailović, Maja
AU  - Trajković, Milena
AU  - Cingel, Aleksandar
AU  - Subotić, Angelina
AU  - Jevremović, Slađana
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6394
AB  - Impatiens hawkeri (Balsaminaceae) je ukrasna biljna vrsta poreklom sa Nove Gvineje. Krioprezervacija predstavlja metod za dugotrajno čuvanje biljnog materijala na -196C u tečnom azotu koji se koristi i za eliminaciju patogena kod biljaka.1 Vrste roda Impatiens su izrazito osetljive na tretmane dehidratacije koji prethode zamrzavanju.2 Takođe, koncentracija saharoze u hranljivim podlogama utiče na morfogenetski potencijal tokom gajenja u kulturi in vitro.3 U ovom radu smo ispitivali uticaj prekulture vrhova izdanaka na povišenoj koncentraciji saharoze (6%), kao i vitamina C na preživljavanje i regeneraciju biljaka posle hemijske dehidratacije vrhova izdanaka sa rastvorima za vitrifikaciju (PVS2 i PVS3). Vrhovi izdanaka I. hawkeri su izrazito osetljivi na tretman sa 100% PVS2 rastvorom. Najveći morfogenetski potencijal posle izlaganja PVS2 rastvoru su pokazali vrhovi tretirani sa 50% PVS2 rastvorom kada je dolazilo samo do kalusiranja vrhova izdanaka. Potpuna regeneracija biljaka posle PVS2 tretmana postignuta je uz dodatak vitamina C u hranljivoj podlozi u koncentraciji od 100 mg/l. Najbolji rezultati preživljavanja i regeneracije biljaka posle odmrzavanja postignuti su korišćenjem metode vitrifikacije sa 100% PVS3 rastvorom u trajanju od 45 min. Izdanci gajeni na hranljivoj podlozi sa 6% saharoze su pokazali veće preživljavanje (49,4%) posle izlaganja ultra niskim temperaturama u odnosu na izdanke gajene na podlogama sa 3% saharoze (26,5 %).
PB  - Belgrade: Serbian Biological Socitey
C3  - Drugi kongres biologa Srbije; 2018 Sep 25-30; Kladovo, Srbija
T1  - Krioprezervacija vrhova izdanaka Impatiens hawkeri W. Bull
SP  - 60
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6394
ER  - 

@conference{
author = "Antonić Reljin, Dragana and Trailović, Maja and Trajković, Milena and Cingel, Aleksandar and Subotić, Angelina and Jevremović, Slađana",
year = "2018",
abstract = "Impatiens hawkeri (Balsaminaceae) je ukrasna biljna vrsta poreklom sa Nove Gvineje. Krioprezervacija predstavlja metod za dugotrajno čuvanje biljnog materijala na -196C u tečnom azotu koji se koristi i za eliminaciju patogena kod biljaka.1 Vrste roda Impatiens su izrazito osetljive na tretmane dehidratacije koji prethode zamrzavanju.2 Takođe, koncentracija saharoze u hranljivim podlogama utiče na morfogenetski potencijal tokom gajenja u kulturi in vitro.3 U ovom radu smo ispitivali uticaj prekulture vrhova izdanaka na povišenoj koncentraciji saharoze (6%), kao i vitamina C na preživljavanje i regeneraciju biljaka posle hemijske dehidratacije vrhova izdanaka sa rastvorima za vitrifikaciju (PVS2 i PVS3). Vrhovi izdanaka I. hawkeri su izrazito osetljivi na tretman sa 100% PVS2 rastvorom. Najveći morfogenetski potencijal posle izlaganja PVS2 rastvoru su pokazali vrhovi tretirani sa 50% PVS2 rastvorom kada je dolazilo samo do kalusiranja vrhova izdanaka. Potpuna regeneracija biljaka posle PVS2 tretmana postignuta je uz dodatak vitamina C u hranljivoj podlozi u koncentraciji od 100 mg/l. Najbolji rezultati preživljavanja i regeneracije biljaka posle odmrzavanja postignuti su korišćenjem metode vitrifikacije sa 100% PVS3 rastvorom u trajanju od 45 min. Izdanci gajeni na hranljivoj podlozi sa 6% saharoze su pokazali veće preživljavanje (49,4%) posle izlaganja ultra niskim temperaturama u odnosu na izdanke gajene na podlogama sa 3% saharoze (26,5 %).",
publisher = "Belgrade: Serbian Biological Socitey",
journal = "Drugi kongres biologa Srbije; 2018 Sep 25-30; Kladovo, Srbija",
title = "Krioprezervacija vrhova izdanaka Impatiens hawkeri W. Bull",
pages = "60",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6394"
}

Antonić Reljin, D., Trailović, M., Trajković, M., Cingel, A., Subotić, A.,& Jevremović, S.. (2018). Krioprezervacija vrhova izdanaka Impatiens hawkeri W. Bull. in Drugi kongres biologa Srbije; 2018 Sep 25-30; Kladovo, Srbija
Belgrade: Serbian Biological Socitey., 60.
https://hdl.handle.net/21.15107/rcub_ibiss_6394

Antonić Reljin D, Trailović M, Trajković M, Cingel A, Subotić A, Jevremović S. Krioprezervacija vrhova izdanaka Impatiens hawkeri W. Bull. in Drugi kongres biologa Srbije; 2018 Sep 25-30; Kladovo, Srbija. 2018;:60.
https://hdl.handle.net/21.15107/rcub_ibiss_6394 .

Antonić Reljin, Dragana, Trailović, Maja, Trajković, Milena, Cingel, Aleksandar, Subotić, Angelina, Jevremović, Slađana, "Krioprezervacija vrhova izdanaka Impatiens hawkeri W. Bull" in Drugi kongres biologa Srbije; 2018 Sep 25-30; Kladovo, Srbija (2018):60,
https://hdl.handle.net/21.15107/rcub_ibiss_6394 .

TY  - CONF
AU  - Antonić, Dragana
AU  - Milošević, Snežana
AU  - Dragićević, Milan
AU  - Simonović, Ana
AU  - Paunović, Danijela
AU  - Momčilović, Ivana
AU  - Subotić, Angelina
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4338
AB  - Impatiens walleriana is an important ornamental plant with a tendency to quickly wilt when
exposed to dehydration, which considerably affects its commercial value. However, Impatiens also
has great potential to rehydrate upon watering. Effective protection of this valuable ornamental
from water stress requires understanding the events associated with dehydration and rehydration
at the molecular level. Dehydrins (DHNs) are highly hydrophilic proteins whose expression in many
plant species has been correlated with several types of abiotic stresses, including drought, salinity,
and cold. Dehydrins comprise Group II of late embryogenesis abundant (LEA) proteins, known to
be associated with plant responses to water deficit. DHNs lack stable tridimensional structures, so
they are considered intrinsically disordered proteins. They are characterized by three conserved
sequence motifs marked as a K (Lys-rich), Y (Tyr-rich) and S (Ser-rich). To study the expression of
Dhn genes in response to drought, we have sequenced I. walleriana transcriptome and identified
IwDhn transcripts from RNA-seq data using HMMER (hmmscan, v3.1b2) and Pfam 31 database.
Quantitative RT-PCR revealed that two IwDhn genes are extremely upregulated in drought, while
one has constitutive expression. It can be speculated that fast rehydration after drought stress is at
least in part result of the protective role of dehydrins.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade
T1  - Expression of dehydrins in Impatiens walleriana exposed to drought
SP  - 74
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4338
ER  - 

@conference{
author = "Antonić, Dragana and Milošević, Snežana and Dragićević, Milan and Simonović, Ana and Paunović, Danijela and Momčilović, Ivana and Subotić, Angelina",
year = "2018",
abstract = "Impatiens walleriana is an important ornamental plant with a tendency to quickly wilt when
exposed to dehydration, which considerably affects its commercial value. However, Impatiens also
has great potential to rehydrate upon watering. Effective protection of this valuable ornamental
from water stress requires understanding the events associated with dehydration and rehydration
at the molecular level. Dehydrins (DHNs) are highly hydrophilic proteins whose expression in many
plant species has been correlated with several types of abiotic stresses, including drought, salinity,
and cold. Dehydrins comprise Group II of late embryogenesis abundant (LEA) proteins, known to
be associated with plant responses to water deficit. DHNs lack stable tridimensional structures, so
they are considered intrinsically disordered proteins. They are characterized by three conserved
sequence motifs marked as a K (Lys-rich), Y (Tyr-rich) and S (Ser-rich). To study the expression of
Dhn genes in response to drought, we have sequenced I. walleriana transcriptome and identified
IwDhn transcripts from RNA-seq data using HMMER (hmmscan, v3.1b2) and Pfam 31 database.
Quantitative RT-PCR revealed that two IwDhn genes are extremely upregulated in drought, while
one has constitutive expression. It can be speculated that fast rehydration after drought stress is at
least in part result of the protective role of dehydrins.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade",
title = "Expression of dehydrins in Impatiens walleriana exposed to drought",
pages = "74",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4338"
}

Antonić, D., Milošević, S., Dragićević, M., Simonović, A., Paunović, D., Momčilović, I.,& Subotić, A.. (2018). Expression of dehydrins in Impatiens walleriana exposed to drought. in 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade
Belgrade: Serbian Plant Physiology Society., 74.
https://hdl.handle.net/21.15107/rcub_ibiss_4338

Antonić D, Milošević S, Dragićević M, Simonović A, Paunović D, Momčilović I, Subotić A. Expression of dehydrins in Impatiens walleriana exposed to drought. in 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade. 2018;:74.
https://hdl.handle.net/21.15107/rcub_ibiss_4338 .

Antonić, Dragana, Milošević, Snežana, Dragićević, Milan, Simonović, Ana, Paunović, Danijela, Momčilović, Ivana, Subotić, Angelina, "Expression of dehydrins in Impatiens walleriana exposed to drought" in 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade (2018):74,
https://hdl.handle.net/21.15107/rcub_ibiss_4338 .

TY  - CONF
AU  - Antonić, Dragana
AU  - Milošević, Snežana
AU  - Pantelić, Danijel
AU  - Simonović, Ana
AU  - Trajković, Milena
AU  - Momčilović, Ivana
AU  - Subotić, Angelina
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4337
AB  - Impatiens walleriana is one of the most popular Impatiens species characterized by fleshy, succulent
leaves and a variety of flower colours. The major problem in production and sale of this
ornamental species is related to its tendency to quickly wilt when drought stressed. Since one of
the most devastating consequences of drought is the onset of oxidative stress, we have studied
the responses of antioxidative enzymes to drought and applied salicylic acid (SA) as a potential
stress-alleviating regulator. The I. walleriana seeds were germinated in a growth chamber under
controlled conditions. Plants (8 weeks old) were divided into four groups: (1) regularly watered
plants (W), (2) regularly watered plants with single application of 2 mM SA (WS), (3) plants exposed
to drought (D), and (4) drought-exposed plants with single application of 2 mM SA (DS). The activities
of antioxidative enzymes including superoxide dismutase (SOD), catalase (CAT) and guaiacol
peroxidase (POX) were assessed in all four groups. Our results show that drought and SA (WS, D,
and DS) increase activity of Cu/ZnSOD and MnSOD in comparison to control (W). Total CAT activity
was increased in D, but not in WS and DS groups; however additional CAT isoforms were observed
in these groups. POX activity was generally high in D and especially in DS group. It can be concluded
that SA improves drought tolerance in I. walleriana grown ex vitro probably by modulating the
activity of antioxidative enzymes and can be used as a drought-ameliorating agent.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade
T1  - Response of antioxidative enzymes to drought and salicylic acid application in Impatiens walleriana grown ex vitro
SP  - 73
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4337
ER  - 

@conference{
author = "Antonić, Dragana and Milošević, Snežana and Pantelić, Danijel and Simonović, Ana and Trajković, Milena and Momčilović, Ivana and Subotić, Angelina",
year = "2018",
abstract = "Impatiens walleriana is one of the most popular Impatiens species characterized by fleshy, succulent
leaves and a variety of flower colours. The major problem in production and sale of this
ornamental species is related to its tendency to quickly wilt when drought stressed. Since one of
the most devastating consequences of drought is the onset of oxidative stress, we have studied
the responses of antioxidative enzymes to drought and applied salicylic acid (SA) as a potential
stress-alleviating regulator. The I. walleriana seeds were germinated in a growth chamber under
controlled conditions. Plants (8 weeks old) were divided into four groups: (1) regularly watered
plants (W), (2) regularly watered plants with single application of 2 mM SA (WS), (3) plants exposed
to drought (D), and (4) drought-exposed plants with single application of 2 mM SA (DS). The activities
of antioxidative enzymes including superoxide dismutase (SOD), catalase (CAT) and guaiacol
peroxidase (POX) were assessed in all four groups. Our results show that drought and SA (WS, D,
and DS) increase activity of Cu/ZnSOD and MnSOD in comparison to control (W). Total CAT activity
was increased in D, but not in WS and DS groups; however additional CAT isoforms were observed
in these groups. POX activity was generally high in D and especially in DS group. It can be concluded
that SA improves drought tolerance in I. walleriana grown ex vitro probably by modulating the
activity of antioxidative enzymes and can be used as a drought-ameliorating agent.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade",
title = "Response of antioxidative enzymes to drought and salicylic acid application in Impatiens walleriana grown ex vitro",
pages = "73",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4337"
}

Antonić, D., Milošević, S., Pantelić, D., Simonović, A., Trajković, M., Momčilović, I.,& Subotić, A.. (2018). Response of antioxidative enzymes to drought and salicylic acid application in Impatiens walleriana grown ex vitro. in 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade
Belgrade: Serbian Plant Physiology Society., 73.
https://hdl.handle.net/21.15107/rcub_ibiss_4337

Antonić D, Milošević S, Pantelić D, Simonović A, Trajković M, Momčilović I, Subotić A. Response of antioxidative enzymes to drought and salicylic acid application in Impatiens walleriana grown ex vitro. in 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade. 2018;:73.
https://hdl.handle.net/21.15107/rcub_ibiss_4337 .

Antonić, Dragana, Milošević, Snežana, Pantelić, Danijel, Simonović, Ana, Trajković, Milena, Momčilović, Ivana, Subotić, Angelina, "Response of antioxidative enzymes to drought and salicylic acid application in Impatiens walleriana grown ex vitro" in 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade (2018):73,
https://hdl.handle.net/21.15107/rcub_ibiss_4337 .

TY  - CONF
AU  - Trajković, Milena
AU  - Jeknić, Zoran
AU  - Antonić Reljin, Dragana
AU  - Subotić, Angelina
AU  - Jevremović, Slađana
AU  - Cingel, Aleksandar
PY  - 2017
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6731
AB  - Introduction: Viola cornuta is a valuable perennial ornamental plant. Development of new traits, such as new flower color with classical breeding suffers from many difficulties, which can be overcome using genetic engineering. With aim to develop protocol for Agrobacterium-mediated transformation of V. cornuta, we used Agrobacterium tumefaciens strain LBA4404 harbouring the superbinary vector pTOK233 carried a GUS reporter gene and hygromycin phosphotransferase selectable marker gene.
Methods: Hypocotyl explants obtained from seedlings were grown on ½MS medium supplemented with 0.1 mg/l 2,4-D and 2.0 mg/l BA for shoot induction. After two days of pre-cultivation, hypocotyl explants were inoculated in bacterial suspension for 15 min and placed on the same culture medium with addition of acetosyringone 100 μM at pH 5.2. After two days of co-cultivation, explants were transferred on shoot induction medium supplemented with cefotaxime and hygromycin B for selection. Regenerated putative transformants were analyzed by PCR for hygromycin phsphotransferase gene presence and by histochemical assay for β-glucuronidase (GUS) activity.
Results: Shoots were obtained within 8 weeks after explants were inoculated with A. tumefaciens, with 2.0% regeneration efficiency. PCR analysis confirmed selectable marker gene presence in twelve out of sixteen (75.0%) independently derived putatively transformed lines that were tested. Additionally, all analyzed lines exhibited a notable β-glucuronidase activity that was not present in untransformed plants.
Conclusion: This is the first report about V. cornuta susceptibility to A. tumefaciens. Presented protocol for genetic transformation can be used for further introduction of desirable traits in V. cornuta cultivars.
PB  - Belgrade: University of Belgrade, Faculty of Biology
C3  - Book of Abstracts: 1st Congress of Molecular Biologists of Serbia: CoMBoS; 2017 Sep 20-21; Belgrade, Serbia
T1  - Agrobacterium-mediated genetic transformation of Viola cornuta
SP  - 83
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6731
ER  - 

@conference{
author = "Trajković, Milena and Jeknić, Zoran and Antonić Reljin, Dragana and Subotić, Angelina and Jevremović, Slađana and Cingel, Aleksandar",
year = "2017",
abstract = "Introduction: Viola cornuta is a valuable perennial ornamental plant. Development of new traits, such as new flower color with classical breeding suffers from many difficulties, which can be overcome using genetic engineering. With aim to develop protocol for Agrobacterium-mediated transformation of V. cornuta, we used Agrobacterium tumefaciens strain LBA4404 harbouring the superbinary vector pTOK233 carried a GUS reporter gene and hygromycin phosphotransferase selectable marker gene.
Methods: Hypocotyl explants obtained from seedlings were grown on ½MS medium supplemented with 0.1 mg/l 2,4-D and 2.0 mg/l BA for shoot induction. After two days of pre-cultivation, hypocotyl explants were inoculated in bacterial suspension for 15 min and placed on the same culture medium with addition of acetosyringone 100 μM at pH 5.2. After two days of co-cultivation, explants were transferred on shoot induction medium supplemented with cefotaxime and hygromycin B for selection. Regenerated putative transformants were analyzed by PCR for hygromycin phsphotransferase gene presence and by histochemical assay for β-glucuronidase (GUS) activity.
Results: Shoots were obtained within 8 weeks after explants were inoculated with A. tumefaciens, with 2.0% regeneration efficiency. PCR analysis confirmed selectable marker gene presence in twelve out of sixteen (75.0%) independently derived putatively transformed lines that were tested. Additionally, all analyzed lines exhibited a notable β-glucuronidase activity that was not present in untransformed plants.
Conclusion: This is the first report about V. cornuta susceptibility to A. tumefaciens. Presented protocol for genetic transformation can be used for further introduction of desirable traits in V. cornuta cultivars.",
publisher = "Belgrade: University of Belgrade, Faculty of Biology",
journal = "Book of Abstracts: 1st Congress of Molecular Biologists of Serbia: CoMBoS; 2017 Sep 20-21; Belgrade, Serbia",
title = "Agrobacterium-mediated genetic transformation of Viola cornuta",
pages = "83",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6731"
}

Trajković, M., Jeknić, Z., Antonić Reljin, D., Subotić, A., Jevremović, S.,& Cingel, A.. (2017). Agrobacterium-mediated genetic transformation of Viola cornuta. in Book of Abstracts: 1st Congress of Molecular Biologists of Serbia: CoMBoS; 2017 Sep 20-21; Belgrade, Serbia
Belgrade: University of Belgrade, Faculty of Biology., 83.
https://hdl.handle.net/21.15107/rcub_ibiss_6731

Trajković M, Jeknić Z, Antonić Reljin D, Subotić A, Jevremović S, Cingel A. Agrobacterium-mediated genetic transformation of Viola cornuta. in Book of Abstracts: 1st Congress of Molecular Biologists of Serbia: CoMBoS; 2017 Sep 20-21; Belgrade, Serbia. 2017;:83.
https://hdl.handle.net/21.15107/rcub_ibiss_6731 .

Trajković, Milena, Jeknić, Zoran, Antonić Reljin, Dragana, Subotić, Angelina, Jevremović, Slađana, Cingel, Aleksandar, "Agrobacterium-mediated genetic transformation of Viola cornuta" in Book of Abstracts: 1st Congress of Molecular Biologists of Serbia: CoMBoS; 2017 Sep 20-21; Belgrade, Serbia (2017):83,
https://hdl.handle.net/21.15107/rcub_ibiss_6731 .

TY  - JOUR
AU  - Antonić Reljin, Dragana
AU  - Trajković, Milena
AU  - Cingel, Aleksandar
AU  - Subotić, Angelina
AU  - Jevremović, Slađana
PY  - 2017
UR  - http://www.journal-pop.org/2017_17_3_95-102.html
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2905
AB  - In vitro-derived petiole and leaf explants of Viola cornuta L. 'Lutea Splendens' were cultured on half-strength Murashige and Skoog (MS) medium with several concentrations of 6-benzylaminopurin (BAP), 6-furfurylaminopurine (KIN), N-phenyl-N'(1,2,3-thidiazol-5-yl) urea (TDZ) or N-(2-chloro-4-pyridyl)-N'phenylurea (CPPU) alone or supplemented with indole-3-acetic acid (IAA) or a-naphthaleneacetic acid (NAA). The most efficient direct adventitious shoot induction (21%) without callus formation was obtained using petiole explants cultured on half-strength MS medium containing 2.02 μM CPPU. Significantly higher shoot multiplication was achieved on medium supplemented with TDZ (7.6 shoots) or CPPU (7.1 shoots) than on medium supplemented with NAA and BAP (2.9 shoots). Shoots were rooted on half-strength MS medium without plant growth regulators.
T2  - Propagation of Ornamental Plants
T1  - Plant regeneration from in vitro-derived leaf and petiole explants of Viola cornuta L. 'Lutea Splendens'
IS  - 3
VL  - 17
SP  - 95
EP  - 102
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_2905
ER  - 

@article{
author = "Antonić Reljin, Dragana and Trajković, Milena and Cingel, Aleksandar and Subotić, Angelina and Jevremović, Slađana",
year = "2017",
abstract = "In vitro-derived petiole and leaf explants of Viola cornuta L. 'Lutea Splendens' were cultured on half-strength Murashige and Skoog (MS) medium with several concentrations of 6-benzylaminopurin (BAP), 6-furfurylaminopurine (KIN), N-phenyl-N'(1,2,3-thidiazol-5-yl) urea (TDZ) or N-(2-chloro-4-pyridyl)-N'phenylurea (CPPU) alone or supplemented with indole-3-acetic acid (IAA) or a-naphthaleneacetic acid (NAA). The most efficient direct adventitious shoot induction (21%) without callus formation was obtained using petiole explants cultured on half-strength MS medium containing 2.02 μM CPPU. Significantly higher shoot multiplication was achieved on medium supplemented with TDZ (7.6 shoots) or CPPU (7.1 shoots) than on medium supplemented with NAA and BAP (2.9 shoots). Shoots were rooted on half-strength MS medium without plant growth regulators.",
journal = "Propagation of Ornamental Plants",
title = "Plant regeneration from in vitro-derived leaf and petiole explants of Viola cornuta L. 'Lutea Splendens'",
number = "3",
volume = "17",
pages = "95-102",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_2905"
}

Antonić Reljin, D., Trajković, M., Cingel, A., Subotić, A.,& Jevremović, S.. (2017). Plant regeneration from in vitro-derived leaf and petiole explants of Viola cornuta L. 'Lutea Splendens'. in Propagation of Ornamental Plants, 17(3), 95-102.
https://hdl.handle.net/21.15107/rcub_ibiss_2905

Antonić Reljin D, Trajković M, Cingel A, Subotić A, Jevremović S. Plant regeneration from in vitro-derived leaf and petiole explants of Viola cornuta L. 'Lutea Splendens'. in Propagation of Ornamental Plants. 2017;17(3):95-102.
https://hdl.handle.net/21.15107/rcub_ibiss_2905 .

Antonić Reljin, Dragana, Trajković, Milena, Cingel, Aleksandar, Subotić, Angelina, Jevremović, Slađana, "Plant regeneration from in vitro-derived leaf and petiole explants of Viola cornuta L. 'Lutea Splendens'" in Propagation of Ornamental Plants, 17, no. 3 (2017):95-102,
https://hdl.handle.net/21.15107/rcub_ibiss_2905 .

TY  - JOUR
AU  - Antonić Reljin, Dragana
AU  - Milošević, Snežana
AU  - Cingel, Aleksandar
AU  - Trajković, Milena
AU  - Trifunović-Momčilov, Milana
AU  - Marković, Marija
AU  - Subotić, Angelina
AU  - Simonović, Ana
PY  - 2016
UR  - http://www.scopus.com/inward/record.url?eid=2-s2.0-84963773069&partnerID=tZOtx3y1
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2470
UR  - https://www.sciencedirect.com/science/article/pii/S0254629915326594?via%3Dihub
AB  - We describe the responses of Impatiens walleriana to polyethylene glycol (PEG)-induced physiological drought and the potential of exogenous salicylic acid (SA) as stress-ameliorating agent. Impatiens shoot culture was established on 16 different media containing 0-3% PEG and 0-3 mM SA. After prolonged drought (60 days), water relation parameters, oxidative stress indicators, and growth responses of the shoots to PEG and/or SA were recorded. PEG reduced growth, fresh weight, the number of developed leaves and shoots (proliferation rate, PR), relative water content, and chlorophyll content. PEG increased leaf water loss (LWL) and caused accumulation of proline, H2O2, and malondialdehyde. The activities of catalase, superoxide dismutase, and peroxidase were increased in response to PEG in a dose-dependent manner, with specific peroxidase isoforms induced by drought. Exogenous SA counteracted the effects of PEG on growth, physiological and biochemical parameters, except on proline accumulation. SA was particularly effective in enhancing PR, preserving LWL, and protecting photosynthetic pigments and membranes from oxidative damage. Proline accumulation was strongly enhanced by both PEG and SA. SA had differential effects on different peroxidase isoforms. SA may be safely used in 2-3 mM concentration for drought protection of Impatiens with no negative effects.
PB  - Elsevier
T2  - South African Journal of Botany
T1  - Effects of exogenous salicylic acid on Impatiens walleriana L. grown in vitro under polyethylene glycol-imposed drought
VL  - 105
DO  - 10.1016/j.sajb.2016.04.002
SP  - 226
EP  - 233
ER  - 

@article{
author = "Antonić Reljin, Dragana and Milošević, Snežana and Cingel, Aleksandar and Trajković, Milena and Trifunović-Momčilov, Milana and Marković, Marija and Subotić, Angelina and Simonović, Ana",
year = "2016",
abstract = "We describe the responses of Impatiens walleriana to polyethylene glycol (PEG)-induced physiological drought and the potential of exogenous salicylic acid (SA) as stress-ameliorating agent. Impatiens shoot culture was established on 16 different media containing 0-3% PEG and 0-3 mM SA. After prolonged drought (60 days), water relation parameters, oxidative stress indicators, and growth responses of the shoots to PEG and/or SA were recorded. PEG reduced growth, fresh weight, the number of developed leaves and shoots (proliferation rate, PR), relative water content, and chlorophyll content. PEG increased leaf water loss (LWL) and caused accumulation of proline, H2O2, and malondialdehyde. The activities of catalase, superoxide dismutase, and peroxidase were increased in response to PEG in a dose-dependent manner, with specific peroxidase isoforms induced by drought. Exogenous SA counteracted the effects of PEG on growth, physiological and biochemical parameters, except on proline accumulation. SA was particularly effective in enhancing PR, preserving LWL, and protecting photosynthetic pigments and membranes from oxidative damage. Proline accumulation was strongly enhanced by both PEG and SA. SA had differential effects on different peroxidase isoforms. SA may be safely used in 2-3 mM concentration for drought protection of Impatiens with no negative effects.",
publisher = "Elsevier",
journal = "South African Journal of Botany",
title = "Effects of exogenous salicylic acid on Impatiens walleriana L. grown in vitro under polyethylene glycol-imposed drought",
volume = "105",
doi = "10.1016/j.sajb.2016.04.002",
pages = "226-233"
}

Antonić Reljin, D., Milošević, S., Cingel, A., Trajković, M., Trifunović-Momčilov, M., Marković, M., Subotić, A.,& Simonović, A.. (2016). Effects of exogenous salicylic acid on Impatiens walleriana L. grown in vitro under polyethylene glycol-imposed drought. in South African Journal of Botany
Elsevier., 105, 226-233.
https://doi.org/10.1016/j.sajb.2016.04.002

Antonić Reljin D, Milošević S, Cingel A, Trajković M, Trifunović-Momčilov M, Marković M, Subotić A, Simonović A. Effects of exogenous salicylic acid on Impatiens walleriana L. grown in vitro under polyethylene glycol-imposed drought. in South African Journal of Botany. 2016;105:226-233.
doi:10.1016/j.sajb.2016.04.002 .

Antonić Reljin, Dragana, Milošević, Snežana, Cingel, Aleksandar, Trajković, Milena, Trifunović-Momčilov, Milana, Marković, Marija, Subotić, Angelina, Simonović, Ana, "Effects of exogenous salicylic acid on Impatiens walleriana L. grown in vitro under polyethylene glycol-imposed drought" in South African Journal of Botany, 105 (2016):226-233,
https://doi.org/10.1016/j.sajb.2016.04.002 . .

TY  - CONF
AU  - Trajković, Milena
AU  - Milošević, Snežana
AU  - Cingel, Aleksandar
AU  - Antonić Reljin, Dragana
AU  - Marković, Marija
AU  - Trifunović-Momčilov, Milana
AU  - Subotić, Angelina
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6782
AB  - Impatiens walleriana is a highly popular ornamental plant worldwide. The major problem in I. walleriana
production, transport and sale display is related to tendency to wilt quickly when drought-stressed thus becoming
undesirable to customers. Salicylic acid (SA) is now considered as an endogenous growth regulator
that influences many plant physiological processes including regulation of plant defense, and plays a significant
role in plant protection against various abiotic stresses. To evaluate the role of exogenously applied SA
against the effect of drought simulated by polyethylene glycol (PEG 8000), growth and physiological parameters
of I. walleriana were analyzed. In vitro developed shoots of I. walleriana were grown on MS media containing
different levels of PEG (0, 1, 2 and 3%) and treated with SA at varying concentrations (0, 1, 2 and 3
mM). Increasing PEG concentrations progressively reduced plant growth parameters (plant height, number
of leaves, fresh weight and proliferation rate) and decreased relative water content, chlorophyll and total pigments
in I. walleriana leaves. In contrast, SA treatments under simulated water deficit conditions enhanced I.
walleriana growth performance. Additionally, a decline in the photosynthetic pigment contents and the level
of water loss in leaves of drought stressed plants were significantly reduced with SA application. Taken together,
these results show that SA improves performance of I. walleriana under stress conditions, suggesting
that exogenous application of SA can be used to increase tolerance of Impatiens plants against water deficit.
PB  - Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade
C3  - Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
T1  - Influence of salicylic acid on morphological and physiological characteristics of Impatiens walleriana L. under water stress induced by polyethylene glycol
SP  - 170
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6782
ER  - 

@conference{
author = "Trajković, Milena and Milošević, Snežana and Cingel, Aleksandar and Antonić Reljin, Dragana and Marković, Marija and Trifunović-Momčilov, Milana and Subotić, Angelina",
year = "2015",
abstract = "Impatiens walleriana is a highly popular ornamental plant worldwide. The major problem in I. walleriana
production, transport and sale display is related to tendency to wilt quickly when drought-stressed thus becoming
undesirable to customers. Salicylic acid (SA) is now considered as an endogenous growth regulator
that influences many plant physiological processes including regulation of plant defense, and plays a significant
role in plant protection against various abiotic stresses. To evaluate the role of exogenously applied SA
against the effect of drought simulated by polyethylene glycol (PEG 8000), growth and physiological parameters
of I. walleriana were analyzed. In vitro developed shoots of I. walleriana were grown on MS media containing
different levels of PEG (0, 1, 2 and 3%) and treated with SA at varying concentrations (0, 1, 2 and 3
mM). Increasing PEG concentrations progressively reduced plant growth parameters (plant height, number
of leaves, fresh weight and proliferation rate) and decreased relative water content, chlorophyll and total pigments
in I. walleriana leaves. In contrast, SA treatments under simulated water deficit conditions enhanced I.
walleriana growth performance. Additionally, a decline in the photosynthetic pigment contents and the level
of water loss in leaves of drought stressed plants were significantly reduced with SA application. Taken together,
these results show that SA improves performance of I. walleriana under stress conditions, suggesting
that exogenous application of SA can be used to increase tolerance of Impatiens plants against water deficit.",
publisher = "Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade",
journal = "Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia",
title = "Influence of salicylic acid on morphological and physiological characteristics of Impatiens walleriana L. under water stress induced by polyethylene glycol",
pages = "170",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6782"
}

Trajković, M., Milošević, S., Cingel, A., Antonić Reljin, D., Marković, M., Trifunović-Momčilov, M.,& Subotić, A.. (2015). Influence of salicylic acid on morphological and physiological characteristics of Impatiens walleriana L. under water stress induced by polyethylene glycol. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade., 170.
https://hdl.handle.net/21.15107/rcub_ibiss_6782

Trajković M, Milošević S, Cingel A, Antonić Reljin D, Marković M, Trifunović-Momčilov M, Subotić A. Influence of salicylic acid on morphological and physiological characteristics of Impatiens walleriana L. under water stress induced by polyethylene glycol. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia. 2015;:170.
https://hdl.handle.net/21.15107/rcub_ibiss_6782 .

Trajković, Milena, Milošević, Snežana, Cingel, Aleksandar, Antonić Reljin, Dragana, Marković, Marija, Trifunović-Momčilov, Milana, Subotić, Angelina, "Influence of salicylic acid on morphological and physiological characteristics of Impatiens walleriana L. under water stress induced by polyethylene glycol" in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia (2015):170,
https://hdl.handle.net/21.15107/rcub_ibiss_6782 .

TY  - CONF
AU  - Antonić Reljin, Dragana
AU  - Marković, Marija
AU  - Simonović, Ana
AU  - Milošević, Snežana
AU  - Trajković, Milena
AU  - Subotić, Angelina
AU  - Jevremović, Slađana
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6780
AB  - Impatiens walleriana is an ornamental plant sensitive to dehydration with tendency to wilt without water.
Proper tissue dehydration is a necessary step in cryopreservation protocols to avoid formation of intracellular
ice crystals which are lethal for plant cells. In order to increase dehydration tolerance of I. walleriana shoots,
we evaluated proline accumulation, a common osmoprotectant, in I. walleriana shoots after two weeks of
culture at several dehydration pretreatments: increased concentration of sucrose (3, 4, 5, 6, and 10%), at different
temperatures (25 ± 2 °C and 4 °C) and different concentration of abscisic acid (ABA, 0, 0.1, 0.2, 0.5, 1, 5
and 10 mg L-1). We observed that graduated concentration of sucrose in culture media increased proline accumulation
in I. walleriana shoots grown under standard conditions, as well as at 4 °C. The highest proline
concentration was recorded in shoots grown on media with 6% sucrose (100 ± 2.3 μmol g-1) at 25 °C which
was more than three times higher than on medium with 3% sucrose (30 ± 0.6 μmol g-1). The highest proline
accumulation in shoots grown at 4 °C was observed at 10% sucrose (88 ± 0.9 μmol g-1) in comparison to
37 ± 0.6 μmol g-1 at 3% sucrose. Addition of ABA in culture medium increased proline concentration in shoots
grown on media with 10 mg L-1 ABA (68 ± 1.2 μmol g-1). Our results suggest that applied pretreatments significantly
increase accumulation of proline and can be helpful in increasing dehydration tolerance of I. walleriana
shoots prior to cryopreservation.
PB  - Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade
C3  - Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
T1  - Proline accumulation in Impatiens walleriana shoots after different dehydration treatments
SP  - 43
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6780
ER  - 

@conference{
author = "Antonić Reljin, Dragana and Marković, Marija and Simonović, Ana and Milošević, Snežana and Trajković, Milena and Subotić, Angelina and Jevremović, Slađana",
year = "2015",
abstract = "Impatiens walleriana is an ornamental plant sensitive to dehydration with tendency to wilt without water.
Proper tissue dehydration is a necessary step in cryopreservation protocols to avoid formation of intracellular
ice crystals which are lethal for plant cells. In order to increase dehydration tolerance of I. walleriana shoots,
we evaluated proline accumulation, a common osmoprotectant, in I. walleriana shoots after two weeks of
culture at several dehydration pretreatments: increased concentration of sucrose (3, 4, 5, 6, and 10%), at different
temperatures (25 ± 2 °C and 4 °C) and different concentration of abscisic acid (ABA, 0, 0.1, 0.2, 0.5, 1, 5
and 10 mg L-1). We observed that graduated concentration of sucrose in culture media increased proline accumulation
in I. walleriana shoots grown under standard conditions, as well as at 4 °C. The highest proline
concentration was recorded in shoots grown on media with 6% sucrose (100 ± 2.3 μmol g-1) at 25 °C which
was more than three times higher than on medium with 3% sucrose (30 ± 0.6 μmol g-1). The highest proline
accumulation in shoots grown at 4 °C was observed at 10% sucrose (88 ± 0.9 μmol g-1) in comparison to
37 ± 0.6 μmol g-1 at 3% sucrose. Addition of ABA in culture medium increased proline concentration in shoots
grown on media with 10 mg L-1 ABA (68 ± 1.2 μmol g-1). Our results suggest that applied pretreatments significantly
increase accumulation of proline and can be helpful in increasing dehydration tolerance of I. walleriana
shoots prior to cryopreservation.",
publisher = "Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade",
journal = "Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia",
title = "Proline accumulation in Impatiens walleriana shoots after different dehydration treatments",
pages = "43",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6780"
}

Antonić Reljin, D., Marković, M., Simonović, A., Milošević, S., Trajković, M., Subotić, A.,& Jevremović, S.. (2015). Proline accumulation in Impatiens walleriana shoots after different dehydration treatments. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade., 43.
https://hdl.handle.net/21.15107/rcub_ibiss_6780

Antonić Reljin D, Marković M, Simonović A, Milošević S, Trajković M, Subotić A, Jevremović S. Proline accumulation in Impatiens walleriana shoots after different dehydration treatments. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia. 2015;:43.
https://hdl.handle.net/21.15107/rcub_ibiss_6780 .

Antonić Reljin, Dragana, Marković, Marija, Simonović, Ana, Milošević, Snežana, Trajković, Milena, Subotić, Angelina, Jevremović, Slađana, "Proline accumulation in Impatiens walleriana shoots after different dehydration treatments" in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia (2015):43,
https://hdl.handle.net/21.15107/rcub_ibiss_6780 .

TY  - CONF
AU  - Trajković, Milena
AU  - Antonić Reljin, Dragana
AU  - Jeknić, Zoran
AU  - Jeknić, Stevan
AU  - Cingel, Aleksandar
AU  - Subotić, Angelina
AU  - Jevremović, Slađana
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6779
AB  - We developed an efficient plant regeneration protocol for a yellow cultivar of Viola cornuta ‘Lutea Splendens’.
Calli and shoots were induced from seedling explants (petiole, leaf, hypocotyls) by one month of culture
on ½-strength MS medium supplemented with 2,4-D (0.1 mg L-1) and BA (2.0 mg L-1). The highest frequency
of shoot induction (12%) was obtained from petiole explants, while the highest frequency of callus formation
(97%) was achieved on hypocotyl explants. The effects of explant origin (top, center, and bottom of hypocotyl)
and light/dark conditions on shoot and callus induction in hypocotyl cultures were also investigated. The
highest shoot induction in hypocotyl cultures (30%) was achieved when explants from the upper section of
the hypocotyls (near the epicotyls) were cultured in light conditions. Shoot multiplication from all explants
was achieved by transferring initially formed shoots to ½strength MS medium supplemented with NAA (0.2
mg L-1), GA3 (2.0 mg L-1), and TDZ (1.0 mg L-1) for four weeks of culture. Developed shoots were then further
multiplied on ½strength MS medium supplemented with NAA (0.5 mg L-1) and BA (1.0 mg L-1). Regenerated
shoots successfully rooted on ½-strength MS medium without growth regulators, acclimatized in a greenhouse
in autumn, and bloomed the following spring. All the regenerated plants exhibited normal morphology
and flower color. This regeneration protocol could be useful for mass propagation using in vitro cultures
and genetic transformation studies using seedling explants.
PB  - Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade
C3  - Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
T1  - Efficient plant regeneration of Viola cornuta ‘Lutea Splendens’ L. using seedling explants
SP  - 25
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6779
ER  - 

@conference{
author = "Trajković, Milena and Antonić Reljin, Dragana and Jeknić, Zoran and Jeknić, Stevan and Cingel, Aleksandar and Subotić, Angelina and Jevremović, Slađana",
year = "2015",
abstract = "We developed an efficient plant regeneration protocol for a yellow cultivar of Viola cornuta ‘Lutea Splendens’.
Calli and shoots were induced from seedling explants (petiole, leaf, hypocotyls) by one month of culture
on ½-strength MS medium supplemented with 2,4-D (0.1 mg L-1) and BA (2.0 mg L-1). The highest frequency
of shoot induction (12%) was obtained from petiole explants, while the highest frequency of callus formation
(97%) was achieved on hypocotyl explants. The effects of explant origin (top, center, and bottom of hypocotyl)
and light/dark conditions on shoot and callus induction in hypocotyl cultures were also investigated. The
highest shoot induction in hypocotyl cultures (30%) was achieved when explants from the upper section of
the hypocotyls (near the epicotyls) were cultured in light conditions. Shoot multiplication from all explants
was achieved by transferring initially formed shoots to ½strength MS medium supplemented with NAA (0.2
mg L-1), GA3 (2.0 mg L-1), and TDZ (1.0 mg L-1) for four weeks of culture. Developed shoots were then further
multiplied on ½strength MS medium supplemented with NAA (0.5 mg L-1) and BA (1.0 mg L-1). Regenerated
shoots successfully rooted on ½-strength MS medium without growth regulators, acclimatized in a greenhouse
in autumn, and bloomed the following spring. All the regenerated plants exhibited normal morphology
and flower color. This regeneration protocol could be useful for mass propagation using in vitro cultures
and genetic transformation studies using seedling explants.",
publisher = "Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade",
journal = "Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia",
title = "Efficient plant regeneration of Viola cornuta ‘Lutea Splendens’ L. using seedling explants",
pages = "25",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6779"
}

Trajković, M., Antonić Reljin, D., Jeknić, Z., Jeknić, S., Cingel, A., Subotić, A.,& Jevremović, S.. (2015). Efficient plant regeneration of Viola cornuta ‘Lutea Splendens’ L. using seedling explants. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade., 25.
https://hdl.handle.net/21.15107/rcub_ibiss_6779

Trajković M, Antonić Reljin D, Jeknić Z, Jeknić S, Cingel A, Subotić A, Jevremović S. Efficient plant regeneration of Viola cornuta ‘Lutea Splendens’ L. using seedling explants. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia. 2015;:25.
https://hdl.handle.net/21.15107/rcub_ibiss_6779 .

Trajković, Milena, Antonić Reljin, Dragana, Jeknić, Zoran, Jeknić, Stevan, Cingel, Aleksandar, Subotić, Angelina, Jevremović, Slađana, "Efficient plant regeneration of Viola cornuta ‘Lutea Splendens’ L. using seedling explants" in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia (2015):25,
https://hdl.handle.net/21.15107/rcub_ibiss_6779 .

TY  - CONF
AU  - Antonić Reljin, Dragana
AU  - Milošević, Snežana
AU  - Trajković, Milena
AU  - Subotić, Angelina
AU  - Jevremović, Slađana
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6781
AB  - Vitrification with plant vitrification solution 2 (PVS2) is commonly used procedure for chemical dehydration
of tissue prior to cryopreservation of the shoot tips. PVS2 contains 30% glycerol (w/v), 15% ethylene glycol
(w/v) and 15% dimethylsulfoxide (DMSO; w/v) in basal culture medium containing 0.4 M sucrose. In order
to develop PVS2-vitrification based protocol for cryopreservation of Impatiens walleriana shoot tips, we tested
the effect of different PVS2 concentrations (100, 80, 60, 50, and 40%), and time of shoot tips exposure to
PVS2 solution (5, 10, 15, 20, 25 and 30 minutes) on survival and plant regeneration prior and after exposure
to liquid nitrogen (LN). Nodal segments of I. walleriana were cultured on plant growth regulator-free MS medium
for three weeks before the shoot tips were isolated. The results have shown that survival of shoot tips
prior to exposure to liquid nitrogen depended on PVS2 concentration and time of exposure to PVS2 solution
and ranged from 100% (lower PVS2 concentrations) to 50% (100% of PVS2, 30 min exposure). After exposure
to LN, the highest survival (77%) was achieved when shoot tips were exposed 25 min to 50% PVS2 solution,
while full strength PVS2 solution with 30 min exposure was harmful when only 26% of cultured shoot tips
survived. The highest plant regeneration (10.3%) of the cryopreserved shoot tips was observed when shoot
tips were exposed 25 minutes to 50% PVS2 solution prior to immersing in LN. Viability of PVS2 treated shoot
tips was determined by staining with fluorescein diacetate.
PB  - Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade
C3  - Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
T1  - Cryopreservation of Impatiens walleriana shoot tips using PVS2 vitrification procedure
SP  - 119
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6781
ER  - 

@conference{
author = "Antonić Reljin, Dragana and Milošević, Snežana and Trajković, Milena and Subotić, Angelina and Jevremović, Slađana",
year = "2015",
abstract = "Vitrification with plant vitrification solution 2 (PVS2) is commonly used procedure for chemical dehydration
of tissue prior to cryopreservation of the shoot tips. PVS2 contains 30% glycerol (w/v), 15% ethylene glycol
(w/v) and 15% dimethylsulfoxide (DMSO; w/v) in basal culture medium containing 0.4 M sucrose. In order
to develop PVS2-vitrification based protocol for cryopreservation of Impatiens walleriana shoot tips, we tested
the effect of different PVS2 concentrations (100, 80, 60, 50, and 40%), and time of shoot tips exposure to
PVS2 solution (5, 10, 15, 20, 25 and 30 minutes) on survival and plant regeneration prior and after exposure
to liquid nitrogen (LN). Nodal segments of I. walleriana were cultured on plant growth regulator-free MS medium
for three weeks before the shoot tips were isolated. The results have shown that survival of shoot tips
prior to exposure to liquid nitrogen depended on PVS2 concentration and time of exposure to PVS2 solution
and ranged from 100% (lower PVS2 concentrations) to 50% (100% of PVS2, 30 min exposure). After exposure
to LN, the highest survival (77%) was achieved when shoot tips were exposed 25 min to 50% PVS2 solution,
while full strength PVS2 solution with 30 min exposure was harmful when only 26% of cultured shoot tips
survived. The highest plant regeneration (10.3%) of the cryopreserved shoot tips was observed when shoot
tips were exposed 25 minutes to 50% PVS2 solution prior to immersing in LN. Viability of PVS2 treated shoot
tips was determined by staining with fluorescein diacetate.",
publisher = "Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade",
journal = "Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia",
title = "Cryopreservation of Impatiens walleriana shoot tips using PVS2 vitrification procedure",
pages = "119",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6781"
}

Antonić Reljin, D., Milošević, S., Trajković, M., Subotić, A.,& Jevremović, S.. (2015). Cryopreservation of Impatiens walleriana shoot tips using PVS2 vitrification procedure. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade., 119.
https://hdl.handle.net/21.15107/rcub_ibiss_6781

Antonić Reljin D, Milošević S, Trajković M, Subotić A, Jevremović S. Cryopreservation of Impatiens walleriana shoot tips using PVS2 vitrification procedure. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia. 2015;:119.
https://hdl.handle.net/21.15107/rcub_ibiss_6781 .

Antonić Reljin, Dragana, Milošević, Snežana, Trajković, Milena, Subotić, Angelina, Jevremović, Slađana, "Cryopreservation of Impatiens walleriana shoot tips using PVS2 vitrification procedure" in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia (2015):119,
https://hdl.handle.net/21.15107/rcub_ibiss_6781 .

TY  - JOUR
AU  - Jevremović, Slađana
AU  - Trajković, Milena
AU  - Jeknić, Zoran
AU  - Trifunović-Momčilov, Milana
AU  - Antonić Reljin, Dragana
AU  - Marković, Marija
AU  - Subotić, Angelina
AU  - Radojević, Ljiljana
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6411
AB  - An efficient propagation protocol by somatic embryogenesis and organogenesis of Balkan endemic iris, Iris reichenbachii, was achieved and clonal fidelity of regenerated plants evaluated. Both regeneration pathways were induced at the same time in zygotic embryo culture on Murashige & Skoog (MS) medium supplemented with 0.5-5.0 mM 2,4-dichlorophenoxy acetic acid (2,4-D) as the sole hormone. Embryogenic calli were further mantained on medium supplemented with 2,4-D and kinetin (Kn; 0.5 and 5.0 mM, respectively). Organogenic calli were selected and further cultured on MS media supplemented with 1-naphthaleneacetic acid (NAA) and benzyladenine (BA; 0.5 and 4.5 mM, respectively) for shoot initiation. Somatic embryos germinated and shoots rooted on MS plant growth regulator-free medium. Plants regenerated by both procceses were succesfully acclimatized in greenhouse conditions and flowered in the following flowering season. Some alterations in flower morphology were detected among plants regenerated by organogenesis. Flow cytometric analysis revealed that plants with altered morphology of flowers had the same ploidy level and genome size as plants collected from the natural habitat. A tetraploid plant was observed in the population of plants regenerated by somatic embryogenesis induced at a high concentration of 2,4-D (10.0 mM).
AB  - Prikazan je efikasan protokol za propagaciju somatskom embriogenezom i organogenezom Balkanske endemične perunike, Iris reichenbachii i procenjena je klonalna indentičnost dobijenih biljaka. Oba načina za regeneraciju u uslovima in vitro su postignuta u kulturi zrelih zigotskih embriona na Murashige & Skoog (MS) hranljivoj podlozi obogaćenoj sa 2,4-dihlorofenoksi sirćetnom kiselinom 0.5-5.0 mM (2,4-D) kao jedinim regulatorom rastenja. Dobijeni embriogeni kalusi su dalje gajeni na hranljivoj podlozi sa 2,4-D i kinetinom (Kn; 0.5 odnosno 5.0 mM). Formirani organogeni kalusi su dalje gajeni na MS hranljivoj podlozi obogaćenoj sa α-naftilsirćetnom kiselinom (NAA) i benziladeninom (BA; 0.5 odnosno 4.5 mM) kada je došlo do formiranja izdanaka. Klijanje somatskih embriona kao i ožiljavanje formiranih izdanaka postignuto je na MS hranljivoj podlozi bez biljnih regulatora rastenja. Biljke dobijene na oba načina su dalje uspešno aklimatizovane na uslove gajenja u stakleniku i cvetale su sledeće godine u proleće. Uočene su neke promene u morfologiji cvetova kod biljaka dobijenih procesom organogeneze. Na osnovu flow-citometrijske analize pokazano je da su biljke sa izmenjenom morfologijom cvetova imale isti nivo ploidnosti i veličinu genoma kao biljke iz prirode. U populaciji biljaka regenerisanih procesom somatske embriogeneze koja je indukovana na podlozi sa visokom koncentracijom 2,4-D (10.0 mM) jedna biljka je bila tetraploidna.
PB  - Belgrade: Institute of Botany and Botanical Garden Jevremovac
T2  - Botanica Serbica
T1  - In vitro propagation of Iris reichenbachii Heuff. and clonal fidelity of regenerated plants
T1  - In vitro propagacija Iris reichenbachii Heuff. i klonalna identičnost dobijenih biljaka
IS  - 2
VL  - 39
SP  - 129
EP  - 136
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6411
ER  - 

@article{
author = "Jevremović, Slađana and Trajković, Milena and Jeknić, Zoran and Trifunović-Momčilov, Milana and Antonić Reljin, Dragana and Marković, Marija and Subotić, Angelina and Radojević, Ljiljana",
year = "2015",
abstract = "An efficient propagation protocol by somatic embryogenesis and organogenesis of Balkan endemic iris, Iris reichenbachii, was achieved and clonal fidelity of regenerated plants evaluated. Both regeneration pathways were induced at the same time in zygotic embryo culture on Murashige & Skoog (MS) medium supplemented with 0.5-5.0 mM 2,4-dichlorophenoxy acetic acid (2,4-D) as the sole hormone. Embryogenic calli were further mantained on medium supplemented with 2,4-D and kinetin (Kn; 0.5 and 5.0 mM, respectively). Organogenic calli were selected and further cultured on MS media supplemented with 1-naphthaleneacetic acid (NAA) and benzyladenine (BA; 0.5 and 4.5 mM, respectively) for shoot initiation. Somatic embryos germinated and shoots rooted on MS plant growth regulator-free medium. Plants regenerated by both procceses were succesfully acclimatized in greenhouse conditions and flowered in the following flowering season. Some alterations in flower morphology were detected among plants regenerated by organogenesis. Flow cytometric analysis revealed that plants with altered morphology of flowers had the same ploidy level and genome size as plants collected from the natural habitat. A tetraploid plant was observed in the population of plants regenerated by somatic embryogenesis induced at a high concentration of 2,4-D (10.0 mM)., Prikazan je efikasan protokol za propagaciju somatskom embriogenezom i organogenezom Balkanske endemične perunike, Iris reichenbachii i procenjena je klonalna indentičnost dobijenih biljaka. Oba načina za regeneraciju u uslovima in vitro su postignuta u kulturi zrelih zigotskih embriona na Murashige & Skoog (MS) hranljivoj podlozi obogaćenoj sa 2,4-dihlorofenoksi sirćetnom kiselinom 0.5-5.0 mM (2,4-D) kao jedinim regulatorom rastenja. Dobijeni embriogeni kalusi su dalje gajeni na hranljivoj podlozi sa 2,4-D i kinetinom (Kn; 0.5 odnosno 5.0 mM). Formirani organogeni kalusi su dalje gajeni na MS hranljivoj podlozi obogaćenoj sa α-naftilsirćetnom kiselinom (NAA) i benziladeninom (BA; 0.5 odnosno 4.5 mM) kada je došlo do formiranja izdanaka. Klijanje somatskih embriona kao i ožiljavanje formiranih izdanaka postignuto je na MS hranljivoj podlozi bez biljnih regulatora rastenja. Biljke dobijene na oba načina su dalje uspešno aklimatizovane na uslove gajenja u stakleniku i cvetale su sledeće godine u proleće. Uočene su neke promene u morfologiji cvetova kod biljaka dobijenih procesom organogeneze. Na osnovu flow-citometrijske analize pokazano je da su biljke sa izmenjenom morfologijom cvetova imale isti nivo ploidnosti i veličinu genoma kao biljke iz prirode. U populaciji biljaka regenerisanih procesom somatske embriogeneze koja je indukovana na podlozi sa visokom koncentracijom 2,4-D (10.0 mM) jedna biljka je bila tetraploidna.",
publisher = "Belgrade: Institute of Botany and Botanical Garden Jevremovac",
journal = "Botanica Serbica",
title = "In vitro propagation of Iris reichenbachii Heuff. and clonal fidelity of regenerated plants, In vitro propagacija Iris reichenbachii Heuff. i klonalna identičnost dobijenih biljaka",
number = "2",
volume = "39",
pages = "129-136",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6411"
}

Jevremović, S., Trajković, M., Jeknić, Z., Trifunović-Momčilov, M., Antonić Reljin, D., Marković, M., Subotić, A.,& Radojević, L.. (2015). In vitro propagation of Iris reichenbachii Heuff. and clonal fidelity of regenerated plants. in Botanica Serbica
Belgrade: Institute of Botany and Botanical Garden Jevremovac., 39(2), 129-136.
https://hdl.handle.net/21.15107/rcub_ibiss_6411

Jevremović S, Trajković M, Jeknić Z, Trifunović-Momčilov M, Antonić Reljin D, Marković M, Subotić A, Radojević L. In vitro propagation of Iris reichenbachii Heuff. and clonal fidelity of regenerated plants. in Botanica Serbica. 2015;39(2):129-136.
https://hdl.handle.net/21.15107/rcub_ibiss_6411 .

Jevremović, Slađana, Trajković, Milena, Jeknić, Zoran, Trifunović-Momčilov, Milana, Antonić Reljin, Dragana, Marković, Marija, Subotić, Angelina, Radojević, Ljiljana, "In vitro propagation of Iris reichenbachii Heuff. and clonal fidelity of regenerated plants" in Botanica Serbica, 39, no. 2 (2015):129-136,
https://hdl.handle.net/21.15107/rcub_ibiss_6411 .

TY  - JOUR
AU  - Milošević, Snežana
AU  - Trajković, Milena
AU  - Antonić Reljin, Dragana
AU  - Cingel, Aleksandar
AU  - Subotić, Angelina
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6210
AB  - Impatiens walleriana L. shoots were inoculated with Agrobacterium rhizogenes
A4M70GUS and the effects of genetic transformation on the catalase (CAT), superoxide
dismutase (SOD) and peroxidase (POX) activities in wounded region of stems and
unwounded leaves were evaluated 10, 24, 240 and 720 hours after inoculation. Following
Agrobacterum infection activities of plant antioxidative enzymes changed in a timedependent manner indicating that dynamic processes occurred during plantAgrobacterium interaction, plant cell transformation and formation of hairy roots.
Appearance of hairy roots on wound sites of shoots was observed ten days after
inoculation with A. rhizogenes and the root induction frequency was 100%. Among
selected hairy root lines significant differences in growth rate and biomass production
were observed and an average 3-fold increase in biomass production was observed for the
best growing hairy root line compared with the untransformed roots. PCR analysis
showed presence of uidA, rolB, rolC and rolD genes in all analyzed I. walleriana L. hairy
root lines, while amplification fragment of rolA gene was detected in 83.3% transformed
lines. Efficient transformation protocol for I. walleriana L described in this work offer
possibilities to generate hairy root cultures for in vitro propagation of plant viruses.
AB  - Izdanci Impatiens walleriana L. su inokulisani sa Agrobacterium rhizogenes A4M70GUS i praćen
je efekat genetičke transformacije na akivnost katalaza (CAT), superoksid dismutaza (SOD) i
peroksidaza (POX) u regionu povrede izdanka kao i u nepovređenim listovima 10, 24, 240 i 720
sati nakon infekcije. Promene u aktivnosti antioksidativnih enzima biljke u zavisnosti od vremena
nakon infekcije ukazuju na dinamične procese koji se odigravaju tokom interakcije biljke i
Agrobakterije, transformacije biljnih ćelija i formiranja “hairy roots” (transformisanih korenova).
Pojava transformisanih korenova je uočena 10 dana nakon inokulacije i učestalost njihove
indukcije iznosila je 100%. Između izabranih “hairy roots” linija postojale su značajne razlike u
stopi rasta i uvećanju biomase, a za transformisanu liniju korenova koja je pokazivala najbolji rast
zabeležen je trostruko veći porast biomase u odnosu na netransformisane korenove. PCR analizom
je potvrđeno prisustvo uidA, rolB, rolC and rolD gena u svim analiziranim linijama
transformisanih korenova I. walleriana L. dok je fragment amplifikacije rolA gena detektovan kod
83.3% transformisanih linija. Efikasni protokol transformacije I. walleriana L koji je opisan u
ovom radu pruža mogućnost stvaranja kultura transformisanih korenova u cilju in vitro
propagacije biljnih virusa.
PB  - Belgrade: Serbian Genetics Society
T2  - Genetika
T1  - Changes of antioxidative enzymes in Impatiens walleriana L. shoots in response to genetic transformation
T1  - Promene antioksidativnih enzima u izdancima Impatiens walleriana L. kao odgovor na genetičku transformaciju
IS  - 1
VL  - 47
DO  - 10.2298/GENSR1501071M
SP  - 71
EP  - 84
ER  - 

@article{
author = "Milošević, Snežana and Trajković, Milena and Antonić Reljin, Dragana and Cingel, Aleksandar and Subotić, Angelina",
year = "2015",
abstract = "Impatiens walleriana L. shoots were inoculated with Agrobacterium rhizogenes
A4M70GUS and the effects of genetic transformation on the catalase (CAT), superoxide
dismutase (SOD) and peroxidase (POX) activities in wounded region of stems and
unwounded leaves were evaluated 10, 24, 240 and 720 hours after inoculation. Following
Agrobacterum infection activities of plant antioxidative enzymes changed in a timedependent manner indicating that dynamic processes occurred during plantAgrobacterium interaction, plant cell transformation and formation of hairy roots.
Appearance of hairy roots on wound sites of shoots was observed ten days after
inoculation with A. rhizogenes and the root induction frequency was 100%. Among
selected hairy root lines significant differences in growth rate and biomass production
were observed and an average 3-fold increase in biomass production was observed for the
best growing hairy root line compared with the untransformed roots. PCR analysis
showed presence of uidA, rolB, rolC and rolD genes in all analyzed I. walleriana L. hairy
root lines, while amplification fragment of rolA gene was detected in 83.3% transformed
lines. Efficient transformation protocol for I. walleriana L described in this work offer
possibilities to generate hairy root cultures for in vitro propagation of plant viruses., Izdanci Impatiens walleriana L. su inokulisani sa Agrobacterium rhizogenes A4M70GUS i praćen
je efekat genetičke transformacije na akivnost katalaza (CAT), superoksid dismutaza (SOD) i
peroksidaza (POX) u regionu povrede izdanka kao i u nepovređenim listovima 10, 24, 240 i 720
sati nakon infekcije. Promene u aktivnosti antioksidativnih enzima biljke u zavisnosti od vremena
nakon infekcije ukazuju na dinamične procese koji se odigravaju tokom interakcije biljke i
Agrobakterije, transformacije biljnih ćelija i formiranja “hairy roots” (transformisanih korenova).
Pojava transformisanih korenova je uočena 10 dana nakon inokulacije i učestalost njihove
indukcije iznosila je 100%. Između izabranih “hairy roots” linija postojale su značajne razlike u
stopi rasta i uvećanju biomase, a za transformisanu liniju korenova koja je pokazivala najbolji rast
zabeležen je trostruko veći porast biomase u odnosu na netransformisane korenove. PCR analizom
je potvrđeno prisustvo uidA, rolB, rolC and rolD gena u svim analiziranim linijama
transformisanih korenova I. walleriana L. dok je fragment amplifikacije rolA gena detektovan kod
83.3% transformisanih linija. Efikasni protokol transformacije I. walleriana L koji je opisan u
ovom radu pruža mogućnost stvaranja kultura transformisanih korenova u cilju in vitro
propagacije biljnih virusa.",
publisher = "Belgrade: Serbian Genetics Society",
journal = "Genetika",
title = "Changes of antioxidative enzymes in Impatiens walleriana L. shoots in response to genetic transformation, Promene antioksidativnih enzima u izdancima Impatiens walleriana L. kao odgovor na genetičku transformaciju",
number = "1",
volume = "47",
doi = "10.2298/GENSR1501071M",
pages = "71-84"
}

Milošević, S., Trajković, M., Antonić Reljin, D., Cingel, A.,& Subotić, A.. (2015). Changes of antioxidative enzymes in Impatiens walleriana L. shoots in response to genetic transformation. in Genetika
Belgrade: Serbian Genetics Society., 47(1), 71-84.
https://doi.org/10.2298/GENSR1501071M

Milošević S, Trajković M, Antonić Reljin D, Cingel A, Subotić A. Changes of antioxidative enzymes in Impatiens walleriana L. shoots in response to genetic transformation. in Genetika. 2015;47(1):71-84.
doi:10.2298/GENSR1501071M .

Milošević, Snežana, Trajković, Milena, Antonić Reljin, Dragana, Cingel, Aleksandar, Subotić, Angelina, "Changes of antioxidative enzymes in Impatiens walleriana L. shoots in response to genetic transformation" in Genetika, 47, no. 1 (2015):71-84,
https://doi.org/10.2298/GENSR1501071M . .

TY  - JOUR
AU  - Tadić, Vojin
AU  - Milošević, Snežana
AU  - Cingel, Aleksandar
AU  - Petrić, Marija
AU  - Trifunović-Momčilov, Milana
AU  - Antonić Reljin, Dragana
AU  - Jovan, Tadic
AU  - Subotić, Angelina
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2122
AB  - Hairy root cultures of lettuce (Lactuca sativa L.) were obtained by
   inoculation of cotyledonary leaves of in vitro lettuce seedlings (cvs.
   Nansen and Ljubljanska ledenka) with Agrobacterium rhizogenes A4M70GUS.
   Approximately in 96.7\% cvs. Nansen and in 91.2\% Ljubljanska ledenka
   inoculated explants produced hairy root when they were incubated on
   Murashige and Skoog (MS) half-strength medium without plant growth
   regulators. A total of 54\% of all hairy root cultures expressed GUS
   activity. Every hairy root represented an independent transformation
   event. Line Ljubljanska ledenka 18 showed the highest biomass (5.5 times
   the biomass of control root). A PCR analysis of the genomic DNA
   confirmed the presence of marker and target genes in 15 hairy roots
   examined.
T2  - Central European Journal of Biology
T1  - Production of hairy root cultures of lettuce (Lactuca sativa L.)
IS  - 12
VL  - 9
DO  - 10.2478/s11535-014-0351-9
SP  - 1196
EP  - 1205
ER  - 

@article{
author = "Tadić, Vojin and Milošević, Snežana and Cingel, Aleksandar and Petrić, Marija and Trifunović-Momčilov, Milana and Antonić Reljin, Dragana and Jovan, Tadic and Subotić, Angelina",
year = "2014",
abstract = "Hairy root cultures of lettuce (Lactuca sativa L.) were obtained by
   inoculation of cotyledonary leaves of in vitro lettuce seedlings (cvs.
   Nansen and Ljubljanska ledenka) with Agrobacterium rhizogenes A4M70GUS.
   Approximately in 96.7\% cvs. Nansen and in 91.2\% Ljubljanska ledenka
   inoculated explants produced hairy root when they were incubated on
   Murashige and Skoog (MS) half-strength medium without plant growth
   regulators. A total of 54\% of all hairy root cultures expressed GUS
   activity. Every hairy root represented an independent transformation
   event. Line Ljubljanska ledenka 18 showed the highest biomass (5.5 times
   the biomass of control root). A PCR analysis of the genomic DNA
   confirmed the presence of marker and target genes in 15 hairy roots
   examined.",
journal = "Central European Journal of Biology",
title = "Production of hairy root cultures of lettuce (Lactuca sativa L.)",
number = "12",
volume = "9",
doi = "10.2478/s11535-014-0351-9",
pages = "1196-1205"
}

Tadić, V., Milošević, S., Cingel, A., Petrić, M., Trifunović-Momčilov, M., Antonić Reljin, D., Jovan, T.,& Subotić, A.. (2014). Production of hairy root cultures of lettuce (Lactuca sativa L.). in Central European Journal of Biology, 9(12), 1196-1205.
https://doi.org/10.2478/s11535-014-0351-9

Tadić V, Milošević S, Cingel A, Petrić M, Trifunović-Momčilov M, Antonić Reljin D, Jovan T, Subotić A. Production of hairy root cultures of lettuce (Lactuca sativa L.). in Central European Journal of Biology. 2014;9(12):1196-1205.
doi:10.2478/s11535-014-0351-9 .

Tadić, Vojin, Milošević, Snežana, Cingel, Aleksandar, Petrić, Marija, Trifunović-Momčilov, Milana, Antonić Reljin, Dragana, Jovan, Tadic, Subotić, Angelina, "Production of hairy root cultures of lettuce (Lactuca sativa L.)" in Central European Journal of Biology, 9, no. 12 (2014):1196-1205,
https://doi.org/10.2478/s11535-014-0351-9 . .