TY  - CONF
AU  - Bondokić, Jasna
AU  - Trajković, Milena
AU  - Dragišić Maksimović, Jelena
AU  - Smailagić, Dijana
AU  - Banjac, Nevena
AU  - Ninković, Slavica
AU  - Stanišić, Mariana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5064
AB  - Phloretin is phenolic compound specifically produced in apple tree species and secreted into the soil as a response to biotic and abiotic factors. Since our previous results indicated roots as the primary site of phloretin phytotoxic action, this study focused on assessment of phloretin effects on the root growth of weed (Chenopodium murale and Lotus corniculatus),  crop (Solanum tuberosum) and woody species (Castanea sativa and Malus × domestica Borkh.). Significant decrease in root biomass production in liquid growth medium enriched with 500 µM phloretin was observed both after 2 and 4 weeks of treatment in all tested species. The percentages of roots growth inhibition were as follows: Chenopodium murale (95% and 89,1%), Lotus corniculatus (69,4% and 81,8%), Solanum tuberosum (88,4% and 84,7%), Castanea sativa (85,6% and 100%) and Malus × domestica Borkh. (65,3% and 87,4%, respectively). Total antioxidant capacity (TAC), total phenolic content (TPC), as well as antioxidant enzymes activities were determined spectrophotometrically in treated and control Chenopodium murale roots after 4 weeks of phloretin treatment. TAC increased 44,1% presumably due to elevated TPC (25,8%) in treated roots. However, there were no significant differences in catalase (CAT), guaiacol peroxidase (POD) and superoxide dismutase (SOD) activities in control and treated roots after 4 weeks of treatment. Increased antioxidant activity in treated roots is presumably the consequence of known phloretin antioxidant potential.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - The effect of phloretin on root growth and antioxidant activity in selected plant species in vitro
SP  - 81
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5064
ER  - 

@conference{
author = "Bondokić, Jasna and Trajković, Milena and Dragišić Maksimović, Jelena and Smailagić, Dijana and Banjac, Nevena and Ninković, Slavica and Stanišić, Mariana",
year = "2022",
abstract = "Phloretin is phenolic compound specifically produced in apple tree species and secreted into the soil as a response to biotic and abiotic factors. Since our previous results indicated roots as the primary site of phloretin phytotoxic action, this study focused on assessment of phloretin effects on the root growth of weed (Chenopodium murale and Lotus corniculatus),  crop (Solanum tuberosum) and woody species (Castanea sativa and Malus × domestica Borkh.). Significant decrease in root biomass production in liquid growth medium enriched with 500 µM phloretin was observed both after 2 and 4 weeks of treatment in all tested species. The percentages of roots growth inhibition were as follows: Chenopodium murale (95% and 89,1%), Lotus corniculatus (69,4% and 81,8%), Solanum tuberosum (88,4% and 84,7%), Castanea sativa (85,6% and 100%) and Malus × domestica Borkh. (65,3% and 87,4%, respectively). Total antioxidant capacity (TAC), total phenolic content (TPC), as well as antioxidant enzymes activities were determined spectrophotometrically in treated and control Chenopodium murale roots after 4 weeks of phloretin treatment. TAC increased 44,1% presumably due to elevated TPC (25,8%) in treated roots. However, there were no significant differences in catalase (CAT), guaiacol peroxidase (POD) and superoxide dismutase (SOD) activities in control and treated roots after 4 weeks of treatment. Increased antioxidant activity in treated roots is presumably the consequence of known phloretin antioxidant potential.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "The effect of phloretin on root growth and antioxidant activity in selected plant species in vitro",
pages = "81",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5064"
}

Bondokić, J., Trajković, M., Dragišić Maksimović, J., Smailagić, D., Banjac, N., Ninković, S.,& Stanišić, M.. (2022). The effect of phloretin on root growth and antioxidant activity in selected plant species in vitro. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 81.
https://hdl.handle.net/21.15107/rcub_ibiss_5064

Bondokić J, Trajković M, Dragišić Maksimović J, Smailagić D, Banjac N, Ninković S, Stanišić M. The effect of phloretin on root growth and antioxidant activity in selected plant species in vitro. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:81.
https://hdl.handle.net/21.15107/rcub_ibiss_5064 .

Bondokić, Jasna, Trajković, Milena, Dragišić Maksimović, Jelena, Smailagić, Dijana, Banjac, Nevena, Ninković, Slavica, Stanišić, Mariana, "The effect of phloretin on root growth and antioxidant activity in selected plant species in vitro" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):81,
https://hdl.handle.net/21.15107/rcub_ibiss_5064 .

TY  - CONF
AU  - Trajković, Milena
AU  - Cingel, Aleksandar
AU  - Jevremović, Slađana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6689
AB  - Boja cveta je jedna od najznačajnijih osobina biljaka čija je osnovna uloga u privlačenju oprašivača. Takođe, boja cvetova određuje i komercijalnu vrednost ukrasnih biljaka. Ova osobina biljaka predstavlja jedan od prvih predmeta istraživanja u oblasti primene genetičkog inženjerstva u hortikulturi. Boja cvetova je određena sadržim tri grupe biljnih pigmenata: anticijanina, karotenoida i betalaina. Veliki napredak u istraživanjima je napravljen na modifikaciji boje cvetova genetičkom modifikacijom biosinteze antocijanina. Sadržaj karotenoida u cvetovima (likopen, lutein, β-karoten, anteraksantin, violaksantin) određuje boju cvetova biljaka od žute do crvene. U radu će biti prikazani rezultati na promeni boje cvetova sitnocvetne ljubičice (Viola cornuta L.) kultivara sa žutim cvetovima genetičkom modifikacijom karotenoida. Genetičkom transformacijom, posredstvom Agrobacterium tumefaciens, genom za kapsantin-kapsorubin sintazu poreklom iz cvetova ljiljana (Llccs) pomoću dva vektora (35S-Llccs i PchsA-Llccs) dobijene su biljke sa promenjenom bojom cvetova. Krunični listići cvetova biljaka su promenjeni iz žute u nijanse narandžaste usled akumulacije novosintetisanog pigmenta kapsantina. Pigment se akumulira u hromoplastima ćelija kruničnih listova usled čega je došlo do promene boje cvetova. Na osnovu ovih rezultata zaključeno je da ektopijska ekspresija gena Llccs pod uticajem oba promotora se može koristi za promenu boje cvetova biljaka.
AB  - Боја цветова је једна од најзначајнијих особина биљака чија је основна улога у привлачењу опрашивача. Такође, боја цветова одређује и комерцијалну вредност украсних биљака. Ова особина биљака представља један од првих предмета истраживања у области примене генетичког инжењерства у хортикултури. Боја цветова је одређена садржајем три групе биљних пигмената: антицијанина, каротеноида и беталаина. Велики напредак у истраживањима је направљен на модификацији боје цветова генетичком модификацијом биосинтезе антоцијанина. Садржај каротеноида у цветовима (ликопен, лутеин,  β-каротен, антераксантин, виолаксантин) одређује боју цветова биљака од жуте до црвене. У раду ће бити приказани резултати на промени боје цветова ситноцветне љубичице (Viola cornuta L.) култивара са жутим цветовима генетичком модификацијом каротеноида. Генетичком трансформацијом, посредством Agrobacterium tumefaciens, геном за капсантин-капсорубин синтазу пореклом из цветова љиљана (Llccs) помоћу два вектора (35S-Llccs и PchsA-Llccs) добијене су биљке са промењеном бојом цветова. Крунични листићи цветова биљака су промењени из жуте у нијансе наранџасте услед акумулације новосинтетисаног пигмента капсантина. Пигмент се акумулира у хромопластима ћелија круничних листића услед чега је дошло до промене боје цветова. На основу ових резултата закључено је да ектопијска експресија гена Llccs под утицајем оба промотора се може користити за промену боје цветова биљака.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Најновија постигнућа на промени боје цветова ситноцветне љубичице (Viola cornuta L.)
T1  - Najnovija postignuća na promeni boje cvetova sitnocvetne ljubičice (Viola cornutaL.)
SP  - 70
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6689
ER  - 

@conference{
author = "Trajković, Milena and Cingel, Aleksandar and Jevremović, Slađana",
year = "2022",
abstract = "Boja cveta je jedna od najznačajnijih osobina biljaka čija je osnovna uloga u privlačenju oprašivača. Takođe, boja cvetova određuje i komercijalnu vrednost ukrasnih biljaka. Ova osobina biljaka predstavlja jedan od prvih predmeta istraživanja u oblasti primene genetičkog inženjerstva u hortikulturi. Boja cvetova je određena sadržim tri grupe biljnih pigmenata: anticijanina, karotenoida i betalaina. Veliki napredak u istraživanjima je napravljen na modifikaciji boje cvetova genetičkom modifikacijom biosinteze antocijanina. Sadržaj karotenoida u cvetovima (likopen, lutein, β-karoten, anteraksantin, violaksantin) određuje boju cvetova biljaka od žute do crvene. U radu će biti prikazani rezultati na promeni boje cvetova sitnocvetne ljubičice (Viola cornuta L.) kultivara sa žutim cvetovima genetičkom modifikacijom karotenoida. Genetičkom transformacijom, posredstvom Agrobacterium tumefaciens, genom za kapsantin-kapsorubin sintazu poreklom iz cvetova ljiljana (Llccs) pomoću dva vektora (35S-Llccs i PchsA-Llccs) dobijene su biljke sa promenjenom bojom cvetova. Krunični listići cvetova biljaka su promenjeni iz žute u nijanse narandžaste usled akumulacije novosintetisanog pigmenta kapsantina. Pigment se akumulira u hromoplastima ćelija kruničnih listova usled čega je došlo do promene boje cvetova. Na osnovu ovih rezultata zaključeno je da ektopijska ekspresija gena Llccs pod uticajem oba promotora se može koristi za promenu boje cvetova biljaka., Боја цветова је једна од најзначајнијих особина биљака чија је основна улога у привлачењу опрашивача. Такође, боја цветова одређује и комерцијалну вредност украсних биљака. Ова особина биљака представља један од првих предмета истраживања у области примене генетичког инжењерства у хортикултури. Боја цветова је одређена садржајем три групе биљних пигмената: антицијанина, каротеноида и беталаина. Велики напредак у истраживањима је направљен на модификацији боје цветова генетичком модификацијом биосинтезе антоцијанина. Садржај каротеноида у цветовима (ликопен, лутеин,  β-каротен, антераксантин, виолаксантин) одређује боју цветова биљака од жуте до црвене. У раду ће бити приказани резултати на промени боје цветова ситноцветне љубичице (Viola cornuta L.) култивара са жутим цветовима генетичком модификацијом каротеноида. Генетичком трансформацијом, посредством Agrobacterium tumefaciens, геном за капсантин-капсорубин синтазу пореклом из цветова љиљана (Llccs) помоћу два вектора (35S-Llccs и PchsA-Llccs) добијене су биљке са промењеном бојом цветова. Крунични листићи цветова биљака су промењени из жуте у нијансе наранџасте услед акумулације новосинтетисаног пигмента капсантина. Пигмент се акумулира у хромопластима ћелија круничних листића услед чега је дошло до промене боје цветова. На основу ових резултата закључено је да ектопијска експресија гена Llccs под утицајем оба промотора се може користити за промену боје цветова биљака.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Најновија постигнућа на промени боје цветова ситноцветне љубичице (Viola cornuta L.), Najnovija postignuća na promeni boje cvetova sitnocvetne ljubičice (Viola cornutaL.)",
pages = "70",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6689"
}

Trajković, M., Cingel, A.,& Jevremović, S.. (2022). Најновија постигнућа на промени боје цветова ситноцветне љубичице (Viola cornuta L.). in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 70.
https://hdl.handle.net/21.15107/rcub_ibiss_6689

Trajković M, Cingel A, Jevremović S. Најновија постигнућа на промени боје цветова ситноцветне љубичице (Viola cornuta L.). in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:70.
https://hdl.handle.net/21.15107/rcub_ibiss_6689 .

Trajković, Milena, Cingel, Aleksandar, Jevremović, Slađana, "Најновија постигнућа на промени боје цветова ситноцветне љубичице (Viola cornuta L.)" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):70,
https://hdl.handle.net/21.15107/rcub_ibiss_6689 .

TY  - CHAP
AU  - Trajković, Milena
AU  - Jevremović, Slađana
AU  - Cingel, Aleksandar
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5302
AB  - U hortikulturi je prisutna stalna potreba za ukrasnim biljkama sa novim karakteristikama, gde boja
cveta predstavlja jednu od najvažnijih osobina koja određuje njihovu komercijalnu vrednost. Sa razvojem
metoda genetičkog inženjeringa otvorena je mogućnost kreiranja biljaka sa željenom bojom cvetova koja
se ne može postići klasičnim ukrštanjem ili mutagenezom. Boja cvetova kod biljaka određena je sadržajem
tri biljna pigmenta: antocijanina, karotenoida i betalaina. Do sada, najveći napredak postignut je genetičkom
modulacijom biosinteze antocijanina. Na ovaj način postignute su nove boje cvetova kod
najmanje 50 ukrasnih vrsta, a neki od tih modifikovanih varijeteta su već dugi niz godina u slobodnoj prodaji.
Međutim, promena boje cveta manipulacijom biosintetskog puta karotenoida je dokumentovana
kod svega nekoliko ukrasnih vrsta i poslednjih godina intenzivirana su istraživanja u tom pravcu. U ovom
radu je razmatran potencijal ovog pristupa, sa posebnim osvrtom na rezultate postignute na promeni boje
cvetova kod kultivara ljubičice uvodjenjem gena za kapsantin-kapsorubin sintazu.
AB  - In horticulture, there is a constant need for ornamental plants with new characteristics, where the flower
color is one of the most important features that determines their commercial value. With the development
of genetic engineering methods, it has been possible to create plants with the desired flower
color which cannot be achieved by classical breeding or mutagenesis. The flower color in plants is determined
by the content of three plant pigments: anthocyanins, carotenoids and betalains. Up to date, the
greatest progress has been made by genetic modulation of anthocyanin biosynthesis. In this way, the new
flower colors have been achieved in at least 50 ornamental species, and some of these modified varieties
have been on market for many years. However, the alteration of flower color by manipulating the carotenoid
biosynthetic pathway has been documented in only a few ornamental species, and the research has
been significantly increased last few years. In this paper, the potential of this approach is considered, with
special reference to the results achieved on flower color alteration of pansy cultivars by introducing the
gene for capsanthin-capsorubin synthase.
PB  - Belgrade: Institute of Molecular Genetics and Genetic Engineering
T2  - Trends in Molecular Biology
T1  - Dosadašnja postignuća na promeni boje cvetova biljaka metaboličkom modulacijom biosinteze karotenoida
T1  - Recent advances in flower color alteration by metabolic manipulation of carotenoid biosynthesis
VL  - 2
SP  - 238
EP  - 253
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5302
ER  - 

@inbook{
author = "Trajković, Milena and Jevremović, Slađana and Cingel, Aleksandar",
year = "2022",
abstract = "U hortikulturi je prisutna stalna potreba za ukrasnim biljkama sa novim karakteristikama, gde boja
cveta predstavlja jednu od najvažnijih osobina koja određuje njihovu komercijalnu vrednost. Sa razvojem
metoda genetičkog inženjeringa otvorena je mogućnost kreiranja biljaka sa željenom bojom cvetova koja
se ne može postići klasičnim ukrštanjem ili mutagenezom. Boja cvetova kod biljaka određena je sadržajem
tri biljna pigmenta: antocijanina, karotenoida i betalaina. Do sada, najveći napredak postignut je genetičkom
modulacijom biosinteze antocijanina. Na ovaj način postignute su nove boje cvetova kod
najmanje 50 ukrasnih vrsta, a neki od tih modifikovanih varijeteta su već dugi niz godina u slobodnoj prodaji.
Međutim, promena boje cveta manipulacijom biosintetskog puta karotenoida je dokumentovana
kod svega nekoliko ukrasnih vrsta i poslednjih godina intenzivirana su istraživanja u tom pravcu. U ovom
radu je razmatran potencijal ovog pristupa, sa posebnim osvrtom na rezultate postignute na promeni boje
cvetova kod kultivara ljubičice uvodjenjem gena za kapsantin-kapsorubin sintazu., In horticulture, there is a constant need for ornamental plants with new characteristics, where the flower
color is one of the most important features that determines their commercial value. With the development
of genetic engineering methods, it has been possible to create plants with the desired flower
color which cannot be achieved by classical breeding or mutagenesis. The flower color in plants is determined
by the content of three plant pigments: anthocyanins, carotenoids and betalains. Up to date, the
greatest progress has been made by genetic modulation of anthocyanin biosynthesis. In this way, the new
flower colors have been achieved in at least 50 ornamental species, and some of these modified varieties
have been on market for many years. However, the alteration of flower color by manipulating the carotenoid
biosynthetic pathway has been documented in only a few ornamental species, and the research has
been significantly increased last few years. In this paper, the potential of this approach is considered, with
special reference to the results achieved on flower color alteration of pansy cultivars by introducing the
gene for capsanthin-capsorubin synthase.",
publisher = "Belgrade: Institute of Molecular Genetics and Genetic Engineering",
journal = "Trends in Molecular Biology",
booktitle = "Dosadašnja postignuća na promeni boje cvetova biljaka metaboličkom modulacijom biosinteze karotenoida, Recent advances in flower color alteration by metabolic manipulation of carotenoid biosynthesis",
volume = "2",
pages = "238-253",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5302"
}

Trajković, M., Jevremović, S.,& Cingel, A.. (2022). Dosadašnja postignuća na promeni boje cvetova biljaka metaboličkom modulacijom biosinteze karotenoida. in Trends in Molecular Biology
Belgrade: Institute of Molecular Genetics and Genetic Engineering., 2, 238-253.
https://hdl.handle.net/21.15107/rcub_ibiss_5302

Trajković M, Jevremović S, Cingel A. Dosadašnja postignuća na promeni boje cvetova biljaka metaboličkom modulacijom biosinteze karotenoida. in Trends in Molecular Biology. 2022;2:238-253.
https://hdl.handle.net/21.15107/rcub_ibiss_5302 .

Trajković, Milena, Jevremović, Slađana, Cingel, Aleksandar, "Dosadašnja postignuća na promeni boje cvetova biljaka metaboličkom modulacijom biosinteze karotenoida" in Trends in Molecular Biology, 2 (2022):238-253,
https://hdl.handle.net/21.15107/rcub_ibiss_5302 .

TY  - JOUR
AU  - Smailagić, Dijana
AU  - Banjac, Nevena
AU  - Ninković, Slavica
AU  - Savić, Jelena
AU  - Ćosić, Tatjana
AU  - Pěnčík, Aleš
AU  - Ćalić, Dušica
AU  - Bogdanović, Milica
AU  - Trajković, Milena
AU  - Stanišić, Mariana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5065
AB  - Apple species are the unique naturally rich source of dihydrochalcones, phenolic compounds with an elusive role in planta, but suggested auto-allelochemical features related to “apple replant disease” (ARD). Our aim was to elucidate the physiological basis of the phytotoxic action of dihydrochalcone phloretin in the model plant Arabidopsis and to promote phloretin as a new prospective eco-friendly phytotoxic compound. Phloretin treatment induced a significant dose-dependent growth retardation and severe morphological abnormalities and agravitropic behavior in Arabidopsis seedlings. Histological examination revealed a reduced starch content in the columella cells and a serious disturbance in root architecture, which resulted in the reduction in length of meristematic and elongation zones. Significantly disturbed auxin metabolome profile in roots with a particularly increased content of IAA accumulated in the lateral parts of the root apex, accompanied by changes in the expression of auxin biosynthetic and transport genes, especially PIN1, PIN3, PIN7, and ABCB1, indicates the role of auxin in physiological basis of phloretin-induced growth retardation. The results reveal a disturbance of auxin homeostasis as the main mechanism of phytotoxic action of phloretin. This mechanism makes phloretin a prospective candidate for an eco-friendly bioherbicide and paves the way for further research of phloretin role in ARD.
PB  - Lausanne: Frontiers Media SA
T2  - Frontiers in Plant Science
T1  - New Insights Into the Activity of Apple Dihydrochalcone Phloretin: Disturbance of Auxin Homeostasis as Physiological Basis of Phloretin Phytotoxic Action
VL  - 13
DO  - 0.3389/fpls.2022.875528
SP  - 875528
ER  - 

@article{
author = "Smailagić, Dijana and Banjac, Nevena and Ninković, Slavica and Savić, Jelena and Ćosić, Tatjana and Pěnčík, Aleš and Ćalić, Dušica and Bogdanović, Milica and Trajković, Milena and Stanišić, Mariana",
year = "2022",
abstract = "Apple species are the unique naturally rich source of dihydrochalcones, phenolic compounds with an elusive role in planta, but suggested auto-allelochemical features related to “apple replant disease” (ARD). Our aim was to elucidate the physiological basis of the phytotoxic action of dihydrochalcone phloretin in the model plant Arabidopsis and to promote phloretin as a new prospective eco-friendly phytotoxic compound. Phloretin treatment induced a significant dose-dependent growth retardation and severe morphological abnormalities and agravitropic behavior in Arabidopsis seedlings. Histological examination revealed a reduced starch content in the columella cells and a serious disturbance in root architecture, which resulted in the reduction in length of meristematic and elongation zones. Significantly disturbed auxin metabolome profile in roots with a particularly increased content of IAA accumulated in the lateral parts of the root apex, accompanied by changes in the expression of auxin biosynthetic and transport genes, especially PIN1, PIN3, PIN7, and ABCB1, indicates the role of auxin in physiological basis of phloretin-induced growth retardation. The results reveal a disturbance of auxin homeostasis as the main mechanism of phytotoxic action of phloretin. This mechanism makes phloretin a prospective candidate for an eco-friendly bioherbicide and paves the way for further research of phloretin role in ARD.",
publisher = "Lausanne: Frontiers Media SA",
journal = "Frontiers in Plant Science",
title = "New Insights Into the Activity of Apple Dihydrochalcone Phloretin: Disturbance of Auxin Homeostasis as Physiological Basis of Phloretin Phytotoxic Action",
volume = "13",
doi = "0.3389/fpls.2022.875528",
pages = "875528"
}

Smailagić, D., Banjac, N., Ninković, S., Savić, J., Ćosić, T., Pěnčík, A., Ćalić, D., Bogdanović, M., Trajković, M.,& Stanišić, M.. (2022). New Insights Into the Activity of Apple Dihydrochalcone Phloretin: Disturbance of Auxin Homeostasis as Physiological Basis of Phloretin Phytotoxic Action. in Frontiers in Plant Science
Lausanne: Frontiers Media SA., 13, 875528.
https://doi.org/0.3389/fpls.2022.875528

Smailagić D, Banjac N, Ninković S, Savić J, Ćosić T, Pěnčík A, Ćalić D, Bogdanović M, Trajković M, Stanišić M. New Insights Into the Activity of Apple Dihydrochalcone Phloretin: Disturbance of Auxin Homeostasis as Physiological Basis of Phloretin Phytotoxic Action. in Frontiers in Plant Science. 2022;13:875528.
doi:0.3389/fpls.2022.875528 .

Smailagić, Dijana, Banjac, Nevena, Ninković, Slavica, Savić, Jelena, Ćosić, Tatjana, Pěnčík, Aleš, Ćalić, Dušica, Bogdanović, Milica, Trajković, Milena, Stanišić, Mariana, "New Insights Into the Activity of Apple Dihydrochalcone Phloretin: Disturbance of Auxin Homeostasis as Physiological Basis of Phloretin Phytotoxic Action" in Frontiers in Plant Science, 13 (2022):875528,
https://doi.org/0.3389/fpls.2022.875528 . .

TY  - JOUR
AU  - Trajković, Milena
AU  - Jevremović, Slađana
AU  - Dragićević, Milan
AU  - Simonović, Ana
AU  - Subotić, Angelina
AU  - Milošević, Snežana
AU  - Cingel, Aleksandar
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4434
AB  - Flower color is an important characteristic that determines the commercial value of ornamental plants. The development of modern biotechnology methods such as genetic engineering
enables the creation of new flower colors that cannot be achieved with classical methods of hybridization or mutational breeding. This is the first report on the successful Agrobacterium-mediated genetic
transformation of Viola cornuta L. The hypocotyl explants of cv. “Lutea Splendens” variety with
yellow flowers were transformed with A. tumefaciens carrying empty pWBVec10a vector (Llccs−) or
pWBVec10a/CaMV 35S::Llccs::TNos vector (Llccs+) for capsanthin/capsorubin synthase gene (Llccs)
from tiger lily (Lilium lancifolium). A comparative study of shoot multiplication, rooting ability during
culture in vitro, as well as phenotypic characteristics of untransformed (control) and transgenic
Llccs− and Llccs+ plants during ex vitro growth and flowering is presented. Successful integration
of Llccs transgene allows the synthesis of red pigment capsanthin in petal cells that gives flowers
different shades of an orange/reddish color. We demonstrate that the ectopic expression of Llccs gene
in ornamental plants, such as V. cornuta “Lutea Splendens” could successfully be used to change
flower color from yellow to different shades of orange.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Alteration of flower color in Viola cornuta cv. ‘Lutea Splendens’ through metabolic engineering of capsanthin/capsorubin synthesis
IS  - 9
VL  - 7
DO  - 10.3390/horticulturae7090324
SP  - 324
ER  - 

@article{
author = "Trajković, Milena and Jevremović, Slađana and Dragićević, Milan and Simonović, Ana and Subotić, Angelina and Milošević, Snežana and Cingel, Aleksandar",
year = "2021",
abstract = "Flower color is an important characteristic that determines the commercial value of ornamental plants. The development of modern biotechnology methods such as genetic engineering
enables the creation of new flower colors that cannot be achieved with classical methods of hybridization or mutational breeding. This is the first report on the successful Agrobacterium-mediated genetic
transformation of Viola cornuta L. The hypocotyl explants of cv. “Lutea Splendens” variety with
yellow flowers were transformed with A. tumefaciens carrying empty pWBVec10a vector (Llccs−) or
pWBVec10a/CaMV 35S::Llccs::TNos vector (Llccs+) for capsanthin/capsorubin synthase gene (Llccs)
from tiger lily (Lilium lancifolium). A comparative study of shoot multiplication, rooting ability during
culture in vitro, as well as phenotypic characteristics of untransformed (control) and transgenic
Llccs− and Llccs+ plants during ex vitro growth and flowering is presented. Successful integration
of Llccs transgene allows the synthesis of red pigment capsanthin in petal cells that gives flowers
different shades of an orange/reddish color. We demonstrate that the ectopic expression of Llccs gene
in ornamental plants, such as V. cornuta “Lutea Splendens” could successfully be used to change
flower color from yellow to different shades of orange.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Alteration of flower color in Viola cornuta cv. ‘Lutea Splendens’ through metabolic engineering of capsanthin/capsorubin synthesis",
number = "9",
volume = "7",
doi = "10.3390/horticulturae7090324",
pages = "324"
}

Trajković, M., Jevremović, S., Dragićević, M., Simonović, A., Subotić, A., Milošević, S.,& Cingel, A.. (2021). Alteration of flower color in Viola cornuta cv. ‘Lutea Splendens’ through metabolic engineering of capsanthin/capsorubin synthesis. in Horticulturae
Basel: MDPI., 7(9), 324.
https://doi.org/10.3390/horticulturae7090324

Trajković M, Jevremović S, Dragićević M, Simonović A, Subotić A, Milošević S, Cingel A. Alteration of flower color in Viola cornuta cv. ‘Lutea Splendens’ through metabolic engineering of capsanthin/capsorubin synthesis. in Horticulturae. 2021;7(9):324.
doi:10.3390/horticulturae7090324 .

Trajković, Milena, Jevremović, Slađana, Dragićević, Milan, Simonović, Ana, Subotić, Angelina, Milošević, Snežana, Cingel, Aleksandar, "Alteration of flower color in Viola cornuta cv. ‘Lutea Splendens’ through metabolic engineering of capsanthin/capsorubin synthesis" in Horticulturae, 7, no. 9 (2021):324,
https://doi.org/10.3390/horticulturae7090324 . .

TY  - THES
AU  - Trajković, Milena
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3684
AB  - Razvijen je protokol za regeneraciju biljaka Viola cornuta L. cv. ’Lutea Splendens’, sitnocvetne ljubičice sa cvetovima žute boje, i genetičku transformaciju genom za kapsantin-kapsorubin-sintazu u cilju promene boje cveta metaboličkom modifikacijom biosinteze karotenoida. Najuspešnija indukcija adventivnih izdanaka dobijena je od eksplantata hipokotila gajenih na ½MS hranljivoj podlozi sa 0,1 mg/l 2,4-D i 2 mg/l BAP u uslovima dugog dana. Indukcija adventivnih izdanaka odvijala se procesom indirektne i direktne organogeneze. Transformisani izdanci dobijeni su genetičkom transformacijom pomoću ”praznog” (Llccs-) i pWBVec10a/CaMV 35S::Llccs::TNos (35S-Llccs) vektora, dok je korišćenjem pWBVec10a/PchsA-Llccs::TNos (PchsA-Llccs) vektora dobijen transformisani kalus. Prisustvo Llccs, hpt i uidA gena u genomu V. cornuta potvrđeno je PCR analizom, dok je qPCR analiza pokazala neujednačen nivo ekspresije Llccs gena u kruničnim listićima cvetova i listovima 35S-Llccs transformisanih linija. Boja kruničnih listića cvetova i žiga tučka promenjena je iz žute, odnosno zelene u nijanse narandžaste boje, usled akumulacije kapsantina, novosintetisanog crvenog pigmenta u hromoplastima 35S-Llccs transformisanih biljaka. Fenotip transformisanih biljaka se značajno razlikovao od fenotipa netransformisanih biljaka. Razvijen je protokol za dugotrajno čuvanje (krioprezervaciju) netransformisanih i transformisanih linija korišćenjem metode vitrifikacije sa PVS3 rastvorom. Oporavak vrhova izdanaka i regeneracija biljaka transformisanih linija (Llccs- i 35S-Llccs) posle krioprezervacije bili su značajno manji u odnosu na oporavak vrhova netransformisanih izdanaka. Nivo ploidnosti biljaka V. cornuta regenerisanih u kulturi in vitro, transformisanih linija kao i biljaka regenerisanih posle krioprezervacije bio je identičan.
AB  - A protocol for the plant regeneration of Viola cornuta L. cv. ’Lutea Splendens’, horned pansy with yellow flowers and the genetic transformation with the capsanthin-capsorubin-synthase gene in order to change flower color by metabolic modification of carotenoid biosynthesis were developed. The most efficient induction of adventitious shoots was obtained from hypocotyl explants grown on ½MS medium with 0,1 mg/l 2,4-D and 2 mg/l BAP under 16h light/8h dark conditions. The induction of adventitious shoots was achieved through a process of indirect and direct organogenesis.Transformed shoots were obtained by genetic transformation using ”empty” (Llccs-) and pWBVec10a/CaMV 35S::Llccs::TNos (35S-Llccs) vectors, while using pWBVec10a/PchsA-Llccs::TNos (PchsA-Llccs) vectors transformed calli were obtained. The presence of the Llccs, hpt and uidA genes in the V. cornuta transformed plants were confirmed by PCR analysis, while qPCR analysis showed an unequal level of Llccs gene expression in the petals and leaves of 35S-Llccs transformed lines. The color of the flower petals and the stigma changed from yellow or green to shades of orange, due to the accumulation of capsanthin, a newly synthesized red pigment in the chromoplasts of 35S-Llccs transformed plants. The phenotype of transformed plants differed significantly from the phenotype of untransformed plants. A protocol for the long-term storage (cryopreservation) of untransformed and transformed lines using the vitrification method with PVS3 solution has been developed. The recovery of shoot tips and regeneration of transformed lines (Llccs- and 35S-Llccs) after cryopreservation was significantly lower compared to the recovery of the shoot tips of the untransformed shoots. The ploidy levels of V. cornuta plants regenerated in culture in vitro, transformed lines and plants recovered after cryopreservation were similar.
PB  - Belgrade: University of Belgrade, Faculty of Biology
T2  - University of Belgrade, Faculty of Biology
T1  - Regeneracija biljaka in vitro i genetička transformacija Viola cornuta (Violaceae) genom za kapsantin-kapsorubin-sintazu
T1  - Plant regeneration in vitro and genetic transformation of Viola cornuta (Violaceae) using capsanthin-capsorubin-synthase gene
SP  - 1
EP  - 143
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3684
ER  - 

@phdthesis{
author = "Trajković, Milena",
year = "2020",
abstract = "Razvijen je protokol za regeneraciju biljaka Viola cornuta L. cv. ’Lutea Splendens’, sitnocvetne ljubičice sa cvetovima žute boje, i genetičku transformaciju genom za kapsantin-kapsorubin-sintazu u cilju promene boje cveta metaboličkom modifikacijom biosinteze karotenoida. Najuspešnija indukcija adventivnih izdanaka dobijena je od eksplantata hipokotila gajenih na ½MS hranljivoj podlozi sa 0,1 mg/l 2,4-D i 2 mg/l BAP u uslovima dugog dana. Indukcija adventivnih izdanaka odvijala se procesom indirektne i direktne organogeneze. Transformisani izdanci dobijeni su genetičkom transformacijom pomoću ”praznog” (Llccs-) i pWBVec10a/CaMV 35S::Llccs::TNos (35S-Llccs) vektora, dok je korišćenjem pWBVec10a/PchsA-Llccs::TNos (PchsA-Llccs) vektora dobijen transformisani kalus. Prisustvo Llccs, hpt i uidA gena u genomu V. cornuta potvrđeno je PCR analizom, dok je qPCR analiza pokazala neujednačen nivo ekspresije Llccs gena u kruničnim listićima cvetova i listovima 35S-Llccs transformisanih linija. Boja kruničnih listića cvetova i žiga tučka promenjena je iz žute, odnosno zelene u nijanse narandžaste boje, usled akumulacije kapsantina, novosintetisanog crvenog pigmenta u hromoplastima 35S-Llccs transformisanih biljaka. Fenotip transformisanih biljaka se značajno razlikovao od fenotipa netransformisanih biljaka. Razvijen je protokol za dugotrajno čuvanje (krioprezervaciju) netransformisanih i transformisanih linija korišćenjem metode vitrifikacije sa PVS3 rastvorom. Oporavak vrhova izdanaka i regeneracija biljaka transformisanih linija (Llccs- i 35S-Llccs) posle krioprezervacije bili su značajno manji u odnosu na oporavak vrhova netransformisanih izdanaka. Nivo ploidnosti biljaka V. cornuta regenerisanih u kulturi in vitro, transformisanih linija kao i biljaka regenerisanih posle krioprezervacije bio je identičan., A protocol for the plant regeneration of Viola cornuta L. cv. ’Lutea Splendens’, horned pansy with yellow flowers and the genetic transformation with the capsanthin-capsorubin-synthase gene in order to change flower color by metabolic modification of carotenoid biosynthesis were developed. The most efficient induction of adventitious shoots was obtained from hypocotyl explants grown on ½MS medium with 0,1 mg/l 2,4-D and 2 mg/l BAP under 16h light/8h dark conditions. The induction of adventitious shoots was achieved through a process of indirect and direct organogenesis.Transformed shoots were obtained by genetic transformation using ”empty” (Llccs-) and pWBVec10a/CaMV 35S::Llccs::TNos (35S-Llccs) vectors, while using pWBVec10a/PchsA-Llccs::TNos (PchsA-Llccs) vectors transformed calli were obtained. The presence of the Llccs, hpt and uidA genes in the V. cornuta transformed plants were confirmed by PCR analysis, while qPCR analysis showed an unequal level of Llccs gene expression in the petals and leaves of 35S-Llccs transformed lines. The color of the flower petals and the stigma changed from yellow or green to shades of orange, due to the accumulation of capsanthin, a newly synthesized red pigment in the chromoplasts of 35S-Llccs transformed plants. The phenotype of transformed plants differed significantly from the phenotype of untransformed plants. A protocol for the long-term storage (cryopreservation) of untransformed and transformed lines using the vitrification method with PVS3 solution has been developed. The recovery of shoot tips and regeneration of transformed lines (Llccs- and 35S-Llccs) after cryopreservation was significantly lower compared to the recovery of the shoot tips of the untransformed shoots. The ploidy levels of V. cornuta plants regenerated in culture in vitro, transformed lines and plants recovered after cryopreservation were similar.",
publisher = "Belgrade: University of Belgrade, Faculty of Biology",
journal = "University of Belgrade, Faculty of Biology",
title = "Regeneracija biljaka in vitro i genetička transformacija Viola cornuta (Violaceae) genom za kapsantin-kapsorubin-sintazu, Plant regeneration in vitro and genetic transformation of Viola cornuta (Violaceae) using capsanthin-capsorubin-synthase gene",
pages = "1-143",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3684"
}

Trajković, M.. (2020). Regeneracija biljaka in vitro i genetička transformacija Viola cornuta (Violaceae) genom za kapsantin-kapsorubin-sintazu. in University of Belgrade, Faculty of Biology
Belgrade: University of Belgrade, Faculty of Biology., 1-143.
https://hdl.handle.net/21.15107/rcub_ibiss_3684

Trajković M. Regeneracija biljaka in vitro i genetička transformacija Viola cornuta (Violaceae) genom za kapsantin-kapsorubin-sintazu. in University of Belgrade, Faculty of Biology. 2020;:1-143.
https://hdl.handle.net/21.15107/rcub_ibiss_3684 .

Trajković, Milena, "Regeneracija biljaka in vitro i genetička transformacija Viola cornuta (Violaceae) genom za kapsantin-kapsorubin-sintazu" in University of Belgrade, Faculty of Biology (2020):1-143,
https://hdl.handle.net/21.15107/rcub_ibiss_3684 .

TY  - JOUR
AU  - Trajković, Milena
AU  - Antonić Reljin, Dragana
AU  - Cingel, Aleksandar
AU  - Ghalawenji, Nabil
AU  - Subotić, Angelina
AU  - Jevremović, Slađana
PY  - 2019
UR  - http://link.springer.com/10.1007/s13205-018-1540-4
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC6314937
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3232
AB  - The aim of this study was to develop a fast, reliable and true-to-type protocol for in vitro plant regeneration and long-term storage of horned pansy (Viola cornuta L). Seed germination over 60% was recorded after 12 weeks of growth at 10 °C or 4 °C. Calli formation and shoot induction were obtained in petiole and hypocotyl culture on half-strength MS mineral salts with full concentration of Na-FeEDTA and vitamins (½MS medium) with 2,4-dichlorophenoxyacetic acid (2,4-D, 0.1 mg/L) and 6-benzylaminopurine (BAP, 2.0 mg/L) and leaf culture on ½MS medium with thidiazuron (TDZ,1.0 mg/L). The highest frequency of adventitious shoot induction (50%) with six shoots/explant was achieved in hypocotyl culture from top hypocotyl segments, close to epicotyl which was grown 8 weeks at 16 h light/8 h dark photoperiod. Subsequent shoot multiplication was achieved on ½MS medium with α-naphthaleneacetic acid (NAA, 0.1 or 0.5 mg/L) and BAP (1.0 mg/L). Rooting of shoots was obtained on ½MS medium with low concentration (0.1 mg/L) of auxins: indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) or NAA, or without growth regulators. In vitro-derived plantlets were acclimatized under greenhouse conditions. All plants developed normally, bloomed and set seeds. Shoot tips were cryopreserved succssefully using modified plant vitrification 3 (PVS3-based vitrification procedure). Cold acclimation for 2 weeks significantly improved shoot regrowth (64%) after thawing in comparison to non-acclimated shoots (39%). Clonal fidelity of regenerated plantlets at ploidy level was confirmed by chromosome counting. The presented protocol can be useful for mass propagation, genetic transformation studies and long-term storage of valuable Viola spp.
T2  - 3 Biotech
T1  - Advancement in protocol for in vitro seed germination, plant regeneration and cryopreservation of Viola cornuta.
IS  - 1
VL  - 9
DO  - 10.1007/s13205-018-1540-4
SP  - 17
ER  - 

@article{
author = "Trajković, Milena and Antonić Reljin, Dragana and Cingel, Aleksandar and Ghalawenji, Nabil and Subotić, Angelina and Jevremović, Slađana",
year = "2019",
abstract = "The aim of this study was to develop a fast, reliable and true-to-type protocol for in vitro plant regeneration and long-term storage of horned pansy (Viola cornuta L). Seed germination over 60% was recorded after 12 weeks of growth at 10 °C or 4 °C. Calli formation and shoot induction were obtained in petiole and hypocotyl culture on half-strength MS mineral salts with full concentration of Na-FeEDTA and vitamins (½MS medium) with 2,4-dichlorophenoxyacetic acid (2,4-D, 0.1 mg/L) and 6-benzylaminopurine (BAP, 2.0 mg/L) and leaf culture on ½MS medium with thidiazuron (TDZ,1.0 mg/L). The highest frequency of adventitious shoot induction (50%) with six shoots/explant was achieved in hypocotyl culture from top hypocotyl segments, close to epicotyl which was grown 8 weeks at 16 h light/8 h dark photoperiod. Subsequent shoot multiplication was achieved on ½MS medium with α-naphthaleneacetic acid (NAA, 0.1 or 0.5 mg/L) and BAP (1.0 mg/L). Rooting of shoots was obtained on ½MS medium with low concentration (0.1 mg/L) of auxins: indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) or NAA, or without growth regulators. In vitro-derived plantlets were acclimatized under greenhouse conditions. All plants developed normally, bloomed and set seeds. Shoot tips were cryopreserved succssefully using modified plant vitrification 3 (PVS3-based vitrification procedure). Cold acclimation for 2 weeks significantly improved shoot regrowth (64%) after thawing in comparison to non-acclimated shoots (39%). Clonal fidelity of regenerated plantlets at ploidy level was confirmed by chromosome counting. The presented protocol can be useful for mass propagation, genetic transformation studies and long-term storage of valuable Viola spp.",
journal = "3 Biotech",
title = "Advancement in protocol for in vitro seed germination, plant regeneration and cryopreservation of Viola cornuta.",
number = "1",
volume = "9",
doi = "10.1007/s13205-018-1540-4",
pages = "17"
}

Trajković, M., Antonić Reljin, D., Cingel, A., Ghalawenji, N., Subotić, A.,& Jevremović, S.. (2019). Advancement in protocol for in vitro seed germination, plant regeneration and cryopreservation of Viola cornuta.. in 3 Biotech, 9(1), 17.
https://doi.org/10.1007/s13205-018-1540-4

Trajković M, Antonić Reljin D, Cingel A, Ghalawenji N, Subotić A, Jevremović S. Advancement in protocol for in vitro seed germination, plant regeneration and cryopreservation of Viola cornuta.. in 3 Biotech. 2019;9(1):17.
doi:10.1007/s13205-018-1540-4 .

Trajković, Milena, Antonić Reljin, Dragana, Cingel, Aleksandar, Ghalawenji, Nabil, Subotić, Angelina, Jevremović, Slađana, "Advancement in protocol for in vitro seed germination, plant regeneration and cryopreservation of Viola cornuta." in 3 Biotech, 9, no. 1 (2019):17,
https://doi.org/10.1007/s13205-018-1540-4 . .

TY  - CONF
AU  - Trajković, Milena
AU  - Antonić Reljin, Dragana
AU  - Trailović, Maja
AU  - Trifunović-Momčilov, Milana
AU  - Subotić, Angelina
AU  - Jevremović, Slađana
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6722
AB  - Cryopreservation represents a suitable method for long term storage of different plant genetic
resources. The aim of this study was to develop protocol for cryopreservation of Viola cornuta
shoot tips using one step freezing method with chemical dehydration of tissue with modified
Plant Vitrification Solutions (PVS2 or PVS3). Shoot tips (1-2 mm) of two-week cold acclimated
shoots were cultured on ½MS medium with 0.3 M sucrose for one day before treatment with loading
solution (2 M glycerol, 0.4 M sucrose) for 30 min. Osmotic dehydration with PVS2 solution (30% glycerol, 15% ethylene glycol and 15% DMSO in liquid ½MS medium with 0.4 M sucrose) were
tested at 0 °C or 24 °C. Osmotic dehydration with PVS3 (50% sucrose, 50% glycerol in liquid ½MS
medium) were tested at 24 °C for 45 min. After the treatment the explants were directly immersed
in liquid nitrogen (LN) for at least one day. Re-warming was performed at 42 °C in water bath
for 2 min. After re-warming, the PVS solutions were replaced with unloading solution containing
1.2 M sucrose for 20 min. Re-warmed shoot tips were cultured on ½MS medium with 0.1 mg L-1
BAP. We observed that PVS2 solution is cytotoxic for V. cornuta shoot tips and cannot be used for
cryopreservation. However, cryopreservation with PVS3 solution was successful, where 71.9-100%
shoot tips survived treatment before immersion to LN and 31-40% survived after re-warming from
LN. Regrowth of cryopreserved shoot tips with new well-formed leaves was obtained after four
weeks of culture.
PB  - Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade, Faculty of Biology, University of Belgrade
C3  - Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
T1  - Cryopreservation of Viola cornuta shoot tips using vitrification procedure
SP  - 91
EP  - 92
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6722
ER  - 

@conference{
author = "Trajković, Milena and Antonić Reljin, Dragana and Trailović, Maja and Trifunović-Momčilov, Milana and Subotić, Angelina and Jevremović, Slađana",
year = "2018",
abstract = "Cryopreservation represents a suitable method for long term storage of different plant genetic
resources. The aim of this study was to develop protocol for cryopreservation of Viola cornuta
shoot tips using one step freezing method with chemical dehydration of tissue with modified
Plant Vitrification Solutions (PVS2 or PVS3). Shoot tips (1-2 mm) of two-week cold acclimated
shoots were cultured on ½MS medium with 0.3 M sucrose for one day before treatment with loading
solution (2 M glycerol, 0.4 M sucrose) for 30 min. Osmotic dehydration with PVS2 solution (30% glycerol, 15% ethylene glycol and 15% DMSO in liquid ½MS medium with 0.4 M sucrose) were
tested at 0 °C or 24 °C. Osmotic dehydration with PVS3 (50% sucrose, 50% glycerol in liquid ½MS
medium) were tested at 24 °C for 45 min. After the treatment the explants were directly immersed
in liquid nitrogen (LN) for at least one day. Re-warming was performed at 42 °C in water bath
for 2 min. After re-warming, the PVS solutions were replaced with unloading solution containing
1.2 M sucrose for 20 min. Re-warmed shoot tips were cultured on ½MS medium with 0.1 mg L-1
BAP. We observed that PVS2 solution is cytotoxic for V. cornuta shoot tips and cannot be used for
cryopreservation. However, cryopreservation with PVS3 solution was successful, where 71.9-100%
shoot tips survived treatment before immersion to LN and 31-40% survived after re-warming from
LN. Regrowth of cryopreserved shoot tips with new well-formed leaves was obtained after four
weeks of culture.",
publisher = "Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade, Faculty of Biology, University of Belgrade",
journal = "Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia",
title = "Cryopreservation of Viola cornuta shoot tips using vitrification procedure",
pages = "91-92",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6722"
}

Trajković, M., Antonić Reljin, D., Trailović, M., Trifunović-Momčilov, M., Subotić, A.,& Jevremović, S.. (2018). Cryopreservation of Viola cornuta shoot tips using vitrification procedure. in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade, Faculty of Biology, University of Belgrade., 91-92.
https://hdl.handle.net/21.15107/rcub_ibiss_6722

Trajković M, Antonić Reljin D, Trailović M, Trifunović-Momčilov M, Subotić A, Jevremović S. Cryopreservation of Viola cornuta shoot tips using vitrification procedure. in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. 2018;:91-92.
https://hdl.handle.net/21.15107/rcub_ibiss_6722 .

Trajković, Milena, Antonić Reljin, Dragana, Trailović, Maja, Trifunović-Momčilov, Milana, Subotić, Angelina, Jevremović, Slađana, "Cryopreservation of Viola cornuta shoot tips using vitrification procedure" in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia (2018):91-92,
https://hdl.handle.net/21.15107/rcub_ibiss_6722 .

TY  - CONF
AU  - Trajković, Milena
AU  - Jeknić, Zoran
AU  - Antonić Reljin, Dragana
AU  - Subotić, Angelina
AU  - Jevremović, Slađana
AU  - Cingel, Aleksandar
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6540
AB  - Viola cornuta L. ‘Lutea Splendens’ is a perennial ornamental plant with small yellow flowers that
naturally grows in the Pyrenees in Spain and France. To develop novel cultivars with orange and
red flower colors, we performed Agrobacterium tumefaciens (LBA4404)-mediated transformation
with the binary vector pWBVec10a/P35S::Llccs::TNos that harbored capsanthin-capsorubin synthase
(Llccs) gene from Lilium lancifolium under the control of CaMV35S constitutive promoter and
the nopaline synthase (Nos) terminator. Capsanthin-capsorubin synthase catalyzes the conversion
of anteraxanthin and violaxanthin, two yellow ubiquitous 5-6-epoxy-xanthophylls, into capsanthin
and capsorubin, two red xanthophylls, respectively. Starting with hypocotyl explants, we developed
a transformation protocol with 0.3% shoot regeneration efficiency. Histochemical assay for
β-glucuronidase (GUS) activity showed uidA reporter gene expression in all putative Llccs-transgenic
shoots. The presence of Llccs transgene, hygromycin phosphotransferase (hpt) selectable
marker gene and uidA (GUS) reporter gene in all putative Llccs-transgenic lines were confirmed
by PCR analysis. This is the first report on Agrobacterium-mediated genetic transformation of V.
cornuta L. with the aim to introduce desirable traits into this species.
PB  - Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade Faculty of Biology, University of Belgrade
C3  - Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
T1  - Agrobacterium-mediated genetic transformation of Viola cornuta L. "Lutea Splendens" with capsanthin-capsorubin synthase gene
SP  - 152
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6540
ER  - 

@conference{
author = "Trajković, Milena and Jeknić, Zoran and Antonić Reljin, Dragana and Subotić, Angelina and Jevremović, Slađana and Cingel, Aleksandar",
year = "2018",
abstract = "Viola cornuta L. ‘Lutea Splendens’ is a perennial ornamental plant with small yellow flowers that
naturally grows in the Pyrenees in Spain and France. To develop novel cultivars with orange and
red flower colors, we performed Agrobacterium tumefaciens (LBA4404)-mediated transformation
with the binary vector pWBVec10a/P35S::Llccs::TNos that harbored capsanthin-capsorubin synthase
(Llccs) gene from Lilium lancifolium under the control of CaMV35S constitutive promoter and
the nopaline synthase (Nos) terminator. Capsanthin-capsorubin synthase catalyzes the conversion
of anteraxanthin and violaxanthin, two yellow ubiquitous 5-6-epoxy-xanthophylls, into capsanthin
and capsorubin, two red xanthophylls, respectively. Starting with hypocotyl explants, we developed
a transformation protocol with 0.3% shoot regeneration efficiency. Histochemical assay for
β-glucuronidase (GUS) activity showed uidA reporter gene expression in all putative Llccs-transgenic
shoots. The presence of Llccs transgene, hygromycin phosphotransferase (hpt) selectable
marker gene and uidA (GUS) reporter gene in all putative Llccs-transgenic lines were confirmed
by PCR analysis. This is the first report on Agrobacterium-mediated genetic transformation of V.
cornuta L. with the aim to introduce desirable traits into this species.",
publisher = "Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade Faculty of Biology, University of Belgrade",
journal = "Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia",
title = "Agrobacterium-mediated genetic transformation of Viola cornuta L. "Lutea Splendens" with capsanthin-capsorubin synthase gene",
pages = "152",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6540"
}

Trajković, M., Jeknić, Z., Antonić Reljin, D., Subotić, A., Jevremović, S.,& Cingel, A.. (2018). Agrobacterium-mediated genetic transformation of Viola cornuta L. "Lutea Splendens" with capsanthin-capsorubin synthase gene. in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade Faculty of Biology, University of Belgrade., 152.
https://hdl.handle.net/21.15107/rcub_ibiss_6540

Trajković M, Jeknić Z, Antonić Reljin D, Subotić A, Jevremović S, Cingel A. Agrobacterium-mediated genetic transformation of Viola cornuta L. "Lutea Splendens" with capsanthin-capsorubin synthase gene. in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. 2018;:152.
https://hdl.handle.net/21.15107/rcub_ibiss_6540 .

Trajković, Milena, Jeknić, Zoran, Antonić Reljin, Dragana, Subotić, Angelina, Jevremović, Slađana, Cingel, Aleksandar, "Agrobacterium-mediated genetic transformation of Viola cornuta L. "Lutea Splendens" with capsanthin-capsorubin synthase gene" in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia (2018):152,
https://hdl.handle.net/21.15107/rcub_ibiss_6540 .

TY  - CONF
AU  - Antonić Reljin, Dragana
AU  - Trailović, Maja
AU  - Trajković, Milena
AU  - Cingel, Aleksandar
AU  - Subotić, Angelina
AU  - Jevremović, Slađana
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6394
AB  - Impatiens hawkeri (Balsaminaceae) je ukrasna biljna vrsta poreklom sa Nove Gvineje. Krioprezervacija predstavlja metod za dugotrajno čuvanje biljnog materijala na -196C u tečnom azotu koji se koristi i za eliminaciju patogena kod biljaka.1 Vrste roda Impatiens su izrazito osetljive na tretmane dehidratacije koji prethode zamrzavanju.2 Takođe, koncentracija saharoze u hranljivim podlogama utiče na morfogenetski potencijal tokom gajenja u kulturi in vitro.3 U ovom radu smo ispitivali uticaj prekulture vrhova izdanaka na povišenoj koncentraciji saharoze (6%), kao i vitamina C na preživljavanje i regeneraciju biljaka posle hemijske dehidratacije vrhova izdanaka sa rastvorima za vitrifikaciju (PVS2 i PVS3). Vrhovi izdanaka I. hawkeri su izrazito osetljivi na tretman sa 100% PVS2 rastvorom. Najveći morfogenetski potencijal posle izlaganja PVS2 rastvoru su pokazali vrhovi tretirani sa 50% PVS2 rastvorom kada je dolazilo samo do kalusiranja vrhova izdanaka. Potpuna regeneracija biljaka posle PVS2 tretmana postignuta je uz dodatak vitamina C u hranljivoj podlozi u koncentraciji od 100 mg/l. Najbolji rezultati preživljavanja i regeneracije biljaka posle odmrzavanja postignuti su korišćenjem metode vitrifikacije sa 100% PVS3 rastvorom u trajanju od 45 min. Izdanci gajeni na hranljivoj podlozi sa 6% saharoze su pokazali veće preživljavanje (49,4%) posle izlaganja ultra niskim temperaturama u odnosu na izdanke gajene na podlogama sa 3% saharoze (26,5 %).
PB  - Belgrade: Serbian Biological Socitey
C3  - Drugi kongres biologa Srbije; 2018 Sep 25-30; Kladovo, Srbija
T1  - Krioprezervacija vrhova izdanaka Impatiens hawkeri W. Bull
SP  - 60
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6394
ER  - 

@conference{
author = "Antonić Reljin, Dragana and Trailović, Maja and Trajković, Milena and Cingel, Aleksandar and Subotić, Angelina and Jevremović, Slađana",
year = "2018",
abstract = "Impatiens hawkeri (Balsaminaceae) je ukrasna biljna vrsta poreklom sa Nove Gvineje. Krioprezervacija predstavlja metod za dugotrajno čuvanje biljnog materijala na -196C u tečnom azotu koji se koristi i za eliminaciju patogena kod biljaka.1 Vrste roda Impatiens su izrazito osetljive na tretmane dehidratacije koji prethode zamrzavanju.2 Takođe, koncentracija saharoze u hranljivim podlogama utiče na morfogenetski potencijal tokom gajenja u kulturi in vitro.3 U ovom radu smo ispitivali uticaj prekulture vrhova izdanaka na povišenoj koncentraciji saharoze (6%), kao i vitamina C na preživljavanje i regeneraciju biljaka posle hemijske dehidratacije vrhova izdanaka sa rastvorima za vitrifikaciju (PVS2 i PVS3). Vrhovi izdanaka I. hawkeri su izrazito osetljivi na tretman sa 100% PVS2 rastvorom. Najveći morfogenetski potencijal posle izlaganja PVS2 rastvoru su pokazali vrhovi tretirani sa 50% PVS2 rastvorom kada je dolazilo samo do kalusiranja vrhova izdanaka. Potpuna regeneracija biljaka posle PVS2 tretmana postignuta je uz dodatak vitamina C u hranljivoj podlozi u koncentraciji od 100 mg/l. Najbolji rezultati preživljavanja i regeneracije biljaka posle odmrzavanja postignuti su korišćenjem metode vitrifikacije sa 100% PVS3 rastvorom u trajanju od 45 min. Izdanci gajeni na hranljivoj podlozi sa 6% saharoze su pokazali veće preživljavanje (49,4%) posle izlaganja ultra niskim temperaturama u odnosu na izdanke gajene na podlogama sa 3% saharoze (26,5 %).",
publisher = "Belgrade: Serbian Biological Socitey",
journal = "Drugi kongres biologa Srbije; 2018 Sep 25-30; Kladovo, Srbija",
title = "Krioprezervacija vrhova izdanaka Impatiens hawkeri W. Bull",
pages = "60",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6394"
}

Antonić Reljin, D., Trailović, M., Trajković, M., Cingel, A., Subotić, A.,& Jevremović, S.. (2018). Krioprezervacija vrhova izdanaka Impatiens hawkeri W. Bull. in Drugi kongres biologa Srbije; 2018 Sep 25-30; Kladovo, Srbija
Belgrade: Serbian Biological Socitey., 60.
https://hdl.handle.net/21.15107/rcub_ibiss_6394

Antonić Reljin D, Trailović M, Trajković M, Cingel A, Subotić A, Jevremović S. Krioprezervacija vrhova izdanaka Impatiens hawkeri W. Bull. in Drugi kongres biologa Srbije; 2018 Sep 25-30; Kladovo, Srbija. 2018;:60.
https://hdl.handle.net/21.15107/rcub_ibiss_6394 .

Antonić Reljin, Dragana, Trailović, Maja, Trajković, Milena, Cingel, Aleksandar, Subotić, Angelina, Jevremović, Slađana, "Krioprezervacija vrhova izdanaka Impatiens hawkeri W. Bull" in Drugi kongres biologa Srbije; 2018 Sep 25-30; Kladovo, Srbija (2018):60,
https://hdl.handle.net/21.15107/rcub_ibiss_6394 .

TY  - CONF
AU  - Antonić, Dragana
AU  - Milošević, Snežana
AU  - Pantelić, Danijel
AU  - Simonović, Ana
AU  - Trajković, Milena
AU  - Momčilović, Ivana
AU  - Subotić, Angelina
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4337
AB  - Impatiens walleriana is one of the most popular Impatiens species characterized by fleshy, succulent
leaves and a variety of flower colours. The major problem in production and sale of this
ornamental species is related to its tendency to quickly wilt when drought stressed. Since one of
the most devastating consequences of drought is the onset of oxidative stress, we have studied
the responses of antioxidative enzymes to drought and applied salicylic acid (SA) as a potential
stress-alleviating regulator. The I. walleriana seeds were germinated in a growth chamber under
controlled conditions. Plants (8 weeks old) were divided into four groups: (1) regularly watered
plants (W), (2) regularly watered plants with single application of 2 mM SA (WS), (3) plants exposed
to drought (D), and (4) drought-exposed plants with single application of 2 mM SA (DS). The activities
of antioxidative enzymes including superoxide dismutase (SOD), catalase (CAT) and guaiacol
peroxidase (POX) were assessed in all four groups. Our results show that drought and SA (WS, D,
and DS) increase activity of Cu/ZnSOD and MnSOD in comparison to control (W). Total CAT activity
was increased in D, but not in WS and DS groups; however additional CAT isoforms were observed
in these groups. POX activity was generally high in D and especially in DS group. It can be concluded
that SA improves drought tolerance in I. walleriana grown ex vitro probably by modulating the
activity of antioxidative enzymes and can be used as a drought-ameliorating agent.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade
T1  - Response of antioxidative enzymes to drought and salicylic acid application in Impatiens walleriana grown ex vitro
SP  - 73
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4337
ER  - 

@conference{
author = "Antonić, Dragana and Milošević, Snežana and Pantelić, Danijel and Simonović, Ana and Trajković, Milena and Momčilović, Ivana and Subotić, Angelina",
year = "2018",
abstract = "Impatiens walleriana is one of the most popular Impatiens species characterized by fleshy, succulent
leaves and a variety of flower colours. The major problem in production and sale of this
ornamental species is related to its tendency to quickly wilt when drought stressed. Since one of
the most devastating consequences of drought is the onset of oxidative stress, we have studied
the responses of antioxidative enzymes to drought and applied salicylic acid (SA) as a potential
stress-alleviating regulator. The I. walleriana seeds were germinated in a growth chamber under
controlled conditions. Plants (8 weeks old) were divided into four groups: (1) regularly watered
plants (W), (2) regularly watered plants with single application of 2 mM SA (WS), (3) plants exposed
to drought (D), and (4) drought-exposed plants with single application of 2 mM SA (DS). The activities
of antioxidative enzymes including superoxide dismutase (SOD), catalase (CAT) and guaiacol
peroxidase (POX) were assessed in all four groups. Our results show that drought and SA (WS, D,
and DS) increase activity of Cu/ZnSOD and MnSOD in comparison to control (W). Total CAT activity
was increased in D, but not in WS and DS groups; however additional CAT isoforms were observed
in these groups. POX activity was generally high in D and especially in DS group. It can be concluded
that SA improves drought tolerance in I. walleriana grown ex vitro probably by modulating the
activity of antioxidative enzymes and can be used as a drought-ameliorating agent.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade",
title = "Response of antioxidative enzymes to drought and salicylic acid application in Impatiens walleriana grown ex vitro",
pages = "73",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4337"
}

Antonić, D., Milošević, S., Pantelić, D., Simonović, A., Trajković, M., Momčilović, I.,& Subotić, A.. (2018). Response of antioxidative enzymes to drought and salicylic acid application in Impatiens walleriana grown ex vitro. in 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade
Belgrade: Serbian Plant Physiology Society., 73.
https://hdl.handle.net/21.15107/rcub_ibiss_4337

Antonić D, Milošević S, Pantelić D, Simonović A, Trajković M, Momčilović I, Subotić A. Response of antioxidative enzymes to drought and salicylic acid application in Impatiens walleriana grown ex vitro. in 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade. 2018;:73.
https://hdl.handle.net/21.15107/rcub_ibiss_4337 .

Antonić, Dragana, Milošević, Snežana, Pantelić, Danijel, Simonović, Ana, Trajković, Milena, Momčilović, Ivana, Subotić, Angelina, "Response of antioxidative enzymes to drought and salicylic acid application in Impatiens walleriana grown ex vitro" in 3rd International Conference on Plant Biology (22nd SPPS Meeting), Belgrade (2018):73,
https://hdl.handle.net/21.15107/rcub_ibiss_4337 .

TY  - CONF
AU  - Trajković, Milena
AU  - Jeknić, Zoran
AU  - Antonić Reljin, Dragana
AU  - Subotić, Angelina
AU  - Jevremović, Slađana
AU  - Cingel, Aleksandar
PY  - 2017
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6731
AB  - Introduction: Viola cornuta is a valuable perennial ornamental plant. Development of new traits, such as new flower color with classical breeding suffers from many difficulties, which can be overcome using genetic engineering. With aim to develop protocol for Agrobacterium-mediated transformation of V. cornuta, we used Agrobacterium tumefaciens strain LBA4404 harbouring the superbinary vector pTOK233 carried a GUS reporter gene and hygromycin phosphotransferase selectable marker gene.
Methods: Hypocotyl explants obtained from seedlings were grown on ½MS medium supplemented with 0.1 mg/l 2,4-D and 2.0 mg/l BA for shoot induction. After two days of pre-cultivation, hypocotyl explants were inoculated in bacterial suspension for 15 min and placed on the same culture medium with addition of acetosyringone 100 μM at pH 5.2. After two days of co-cultivation, explants were transferred on shoot induction medium supplemented with cefotaxime and hygromycin B for selection. Regenerated putative transformants were analyzed by PCR for hygromycin phsphotransferase gene presence and by histochemical assay for β-glucuronidase (GUS) activity.
Results: Shoots were obtained within 8 weeks after explants were inoculated with A. tumefaciens, with 2.0% regeneration efficiency. PCR analysis confirmed selectable marker gene presence in twelve out of sixteen (75.0%) independently derived putatively transformed lines that were tested. Additionally, all analyzed lines exhibited a notable β-glucuronidase activity that was not present in untransformed plants.
Conclusion: This is the first report about V. cornuta susceptibility to A. tumefaciens. Presented protocol for genetic transformation can be used for further introduction of desirable traits in V. cornuta cultivars.
PB  - Belgrade: University of Belgrade, Faculty of Biology
C3  - Book of Abstracts: 1st Congress of Molecular Biologists of Serbia: CoMBoS; 2017 Sep 20-21; Belgrade, Serbia
T1  - Agrobacterium-mediated genetic transformation of Viola cornuta
SP  - 83
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6731
ER  - 

@conference{
author = "Trajković, Milena and Jeknić, Zoran and Antonić Reljin, Dragana and Subotić, Angelina and Jevremović, Slađana and Cingel, Aleksandar",
year = "2017",
abstract = "Introduction: Viola cornuta is a valuable perennial ornamental plant. Development of new traits, such as new flower color with classical breeding suffers from many difficulties, which can be overcome using genetic engineering. With aim to develop protocol for Agrobacterium-mediated transformation of V. cornuta, we used Agrobacterium tumefaciens strain LBA4404 harbouring the superbinary vector pTOK233 carried a GUS reporter gene and hygromycin phosphotransferase selectable marker gene.
Methods: Hypocotyl explants obtained from seedlings were grown on ½MS medium supplemented with 0.1 mg/l 2,4-D and 2.0 mg/l BA for shoot induction. After two days of pre-cultivation, hypocotyl explants were inoculated in bacterial suspension for 15 min and placed on the same culture medium with addition of acetosyringone 100 μM at pH 5.2. After two days of co-cultivation, explants were transferred on shoot induction medium supplemented with cefotaxime and hygromycin B for selection. Regenerated putative transformants were analyzed by PCR for hygromycin phsphotransferase gene presence and by histochemical assay for β-glucuronidase (GUS) activity.
Results: Shoots were obtained within 8 weeks after explants were inoculated with A. tumefaciens, with 2.0% regeneration efficiency. PCR analysis confirmed selectable marker gene presence in twelve out of sixteen (75.0%) independently derived putatively transformed lines that were tested. Additionally, all analyzed lines exhibited a notable β-glucuronidase activity that was not present in untransformed plants.
Conclusion: This is the first report about V. cornuta susceptibility to A. tumefaciens. Presented protocol for genetic transformation can be used for further introduction of desirable traits in V. cornuta cultivars.",
publisher = "Belgrade: University of Belgrade, Faculty of Biology",
journal = "Book of Abstracts: 1st Congress of Molecular Biologists of Serbia: CoMBoS; 2017 Sep 20-21; Belgrade, Serbia",
title = "Agrobacterium-mediated genetic transformation of Viola cornuta",
pages = "83",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6731"
}

Trajković, M., Jeknić, Z., Antonić Reljin, D., Subotić, A., Jevremović, S.,& Cingel, A.. (2017). Agrobacterium-mediated genetic transformation of Viola cornuta. in Book of Abstracts: 1st Congress of Molecular Biologists of Serbia: CoMBoS; 2017 Sep 20-21; Belgrade, Serbia
Belgrade: University of Belgrade, Faculty of Biology., 83.
https://hdl.handle.net/21.15107/rcub_ibiss_6731

Trajković M, Jeknić Z, Antonić Reljin D, Subotić A, Jevremović S, Cingel A. Agrobacterium-mediated genetic transformation of Viola cornuta. in Book of Abstracts: 1st Congress of Molecular Biologists of Serbia: CoMBoS; 2017 Sep 20-21; Belgrade, Serbia. 2017;:83.
https://hdl.handle.net/21.15107/rcub_ibiss_6731 .

Trajković, Milena, Jeknić, Zoran, Antonić Reljin, Dragana, Subotić, Angelina, Jevremović, Slađana, Cingel, Aleksandar, "Agrobacterium-mediated genetic transformation of Viola cornuta" in Book of Abstracts: 1st Congress of Molecular Biologists of Serbia: CoMBoS; 2017 Sep 20-21; Belgrade, Serbia (2017):83,
https://hdl.handle.net/21.15107/rcub_ibiss_6731 .

TY  - JOUR
AU  - Antonić Reljin, Dragana
AU  - Trajković, Milena
AU  - Cingel, Aleksandar
AU  - Subotić, Angelina
AU  - Jevremović, Slađana
PY  - 2017
UR  - http://www.journal-pop.org/2017_17_3_95-102.html
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2905
AB  - In vitro-derived petiole and leaf explants of Viola cornuta L. 'Lutea Splendens' were cultured on half-strength Murashige and Skoog (MS) medium with several concentrations of 6-benzylaminopurin (BAP), 6-furfurylaminopurine (KIN), N-phenyl-N'(1,2,3-thidiazol-5-yl) urea (TDZ) or N-(2-chloro-4-pyridyl)-N'phenylurea (CPPU) alone or supplemented with indole-3-acetic acid (IAA) or a-naphthaleneacetic acid (NAA). The most efficient direct adventitious shoot induction (21%) without callus formation was obtained using petiole explants cultured on half-strength MS medium containing 2.02 μM CPPU. Significantly higher shoot multiplication was achieved on medium supplemented with TDZ (7.6 shoots) or CPPU (7.1 shoots) than on medium supplemented with NAA and BAP (2.9 shoots). Shoots were rooted on half-strength MS medium without plant growth regulators.
T2  - Propagation of Ornamental Plants
T1  - Plant regeneration from in vitro-derived leaf and petiole explants of Viola cornuta L. 'Lutea Splendens'
IS  - 3
VL  - 17
SP  - 95
EP  - 102
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_2905
ER  - 

@article{
author = "Antonić Reljin, Dragana and Trajković, Milena and Cingel, Aleksandar and Subotić, Angelina and Jevremović, Slađana",
year = "2017",
abstract = "In vitro-derived petiole and leaf explants of Viola cornuta L. 'Lutea Splendens' were cultured on half-strength Murashige and Skoog (MS) medium with several concentrations of 6-benzylaminopurin (BAP), 6-furfurylaminopurine (KIN), N-phenyl-N'(1,2,3-thidiazol-5-yl) urea (TDZ) or N-(2-chloro-4-pyridyl)-N'phenylurea (CPPU) alone or supplemented with indole-3-acetic acid (IAA) or a-naphthaleneacetic acid (NAA). The most efficient direct adventitious shoot induction (21%) without callus formation was obtained using petiole explants cultured on half-strength MS medium containing 2.02 μM CPPU. Significantly higher shoot multiplication was achieved on medium supplemented with TDZ (7.6 shoots) or CPPU (7.1 shoots) than on medium supplemented with NAA and BAP (2.9 shoots). Shoots were rooted on half-strength MS medium without plant growth regulators.",
journal = "Propagation of Ornamental Plants",
title = "Plant regeneration from in vitro-derived leaf and petiole explants of Viola cornuta L. 'Lutea Splendens'",
number = "3",
volume = "17",
pages = "95-102",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_2905"
}

Antonić Reljin, D., Trajković, M., Cingel, A., Subotić, A.,& Jevremović, S.. (2017). Plant regeneration from in vitro-derived leaf and petiole explants of Viola cornuta L. 'Lutea Splendens'. in Propagation of Ornamental Plants, 17(3), 95-102.
https://hdl.handle.net/21.15107/rcub_ibiss_2905

Antonić Reljin D, Trajković M, Cingel A, Subotić A, Jevremović S. Plant regeneration from in vitro-derived leaf and petiole explants of Viola cornuta L. 'Lutea Splendens'. in Propagation of Ornamental Plants. 2017;17(3):95-102.
https://hdl.handle.net/21.15107/rcub_ibiss_2905 .

Antonić Reljin, Dragana, Trajković, Milena, Cingel, Aleksandar, Subotić, Angelina, Jevremović, Slađana, "Plant regeneration from in vitro-derived leaf and petiole explants of Viola cornuta L. 'Lutea Splendens'" in Propagation of Ornamental Plants, 17, no. 3 (2017):95-102,
https://hdl.handle.net/21.15107/rcub_ibiss_2905 .

TY  - JOUR
AU  - Antonić Reljin, Dragana
AU  - Milošević, Snežana
AU  - Cingel, Aleksandar
AU  - Trajković, Milena
AU  - Trifunović-Momčilov, Milana
AU  - Marković, Marija
AU  - Subotić, Angelina
AU  - Simonović, Ana
PY  - 2016
UR  - http://www.scopus.com/inward/record.url?eid=2-s2.0-84963773069&partnerID=tZOtx3y1
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2470
UR  - https://www.sciencedirect.com/science/article/pii/S0254629915326594?via%3Dihub
AB  - We describe the responses of Impatiens walleriana to polyethylene glycol (PEG)-induced physiological drought and the potential of exogenous salicylic acid (SA) as stress-ameliorating agent. Impatiens shoot culture was established on 16 different media containing 0-3% PEG and 0-3 mM SA. After prolonged drought (60 days), water relation parameters, oxidative stress indicators, and growth responses of the shoots to PEG and/or SA were recorded. PEG reduced growth, fresh weight, the number of developed leaves and shoots (proliferation rate, PR), relative water content, and chlorophyll content. PEG increased leaf water loss (LWL) and caused accumulation of proline, H2O2, and malondialdehyde. The activities of catalase, superoxide dismutase, and peroxidase were increased in response to PEG in a dose-dependent manner, with specific peroxidase isoforms induced by drought. Exogenous SA counteracted the effects of PEG on growth, physiological and biochemical parameters, except on proline accumulation. SA was particularly effective in enhancing PR, preserving LWL, and protecting photosynthetic pigments and membranes from oxidative damage. Proline accumulation was strongly enhanced by both PEG and SA. SA had differential effects on different peroxidase isoforms. SA may be safely used in 2-3 mM concentration for drought protection of Impatiens with no negative effects.
PB  - Elsevier
T2  - South African Journal of Botany
T1  - Effects of exogenous salicylic acid on Impatiens walleriana L. grown in vitro under polyethylene glycol-imposed drought
VL  - 105
DO  - 10.1016/j.sajb.2016.04.002
SP  - 226
EP  - 233
ER  - 

@article{
author = "Antonić Reljin, Dragana and Milošević, Snežana and Cingel, Aleksandar and Trajković, Milena and Trifunović-Momčilov, Milana and Marković, Marija and Subotić, Angelina and Simonović, Ana",
year = "2016",
abstract = "We describe the responses of Impatiens walleriana to polyethylene glycol (PEG)-induced physiological drought and the potential of exogenous salicylic acid (SA) as stress-ameliorating agent. Impatiens shoot culture was established on 16 different media containing 0-3% PEG and 0-3 mM SA. After prolonged drought (60 days), water relation parameters, oxidative stress indicators, and growth responses of the shoots to PEG and/or SA were recorded. PEG reduced growth, fresh weight, the number of developed leaves and shoots (proliferation rate, PR), relative water content, and chlorophyll content. PEG increased leaf water loss (LWL) and caused accumulation of proline, H2O2, and malondialdehyde. The activities of catalase, superoxide dismutase, and peroxidase were increased in response to PEG in a dose-dependent manner, with specific peroxidase isoforms induced by drought. Exogenous SA counteracted the effects of PEG on growth, physiological and biochemical parameters, except on proline accumulation. SA was particularly effective in enhancing PR, preserving LWL, and protecting photosynthetic pigments and membranes from oxidative damage. Proline accumulation was strongly enhanced by both PEG and SA. SA had differential effects on different peroxidase isoforms. SA may be safely used in 2-3 mM concentration for drought protection of Impatiens with no negative effects.",
publisher = "Elsevier",
journal = "South African Journal of Botany",
title = "Effects of exogenous salicylic acid on Impatiens walleriana L. grown in vitro under polyethylene glycol-imposed drought",
volume = "105",
doi = "10.1016/j.sajb.2016.04.002",
pages = "226-233"
}

Antonić Reljin, D., Milošević, S., Cingel, A., Trajković, M., Trifunović-Momčilov, M., Marković, M., Subotić, A.,& Simonović, A.. (2016). Effects of exogenous salicylic acid on Impatiens walleriana L. grown in vitro under polyethylene glycol-imposed drought. in South African Journal of Botany
Elsevier., 105, 226-233.
https://doi.org/10.1016/j.sajb.2016.04.002

Antonić Reljin D, Milošević S, Cingel A, Trajković M, Trifunović-Momčilov M, Marković M, Subotić A, Simonović A. Effects of exogenous salicylic acid on Impatiens walleriana L. grown in vitro under polyethylene glycol-imposed drought. in South African Journal of Botany. 2016;105:226-233.
doi:10.1016/j.sajb.2016.04.002 .

Antonić Reljin, Dragana, Milošević, Snežana, Cingel, Aleksandar, Trajković, Milena, Trifunović-Momčilov, Milana, Marković, Marija, Subotić, Angelina, Simonović, Ana, "Effects of exogenous salicylic acid on Impatiens walleriana L. grown in vitro under polyethylene glycol-imposed drought" in South African Journal of Botany, 105 (2016):226-233,
https://doi.org/10.1016/j.sajb.2016.04.002 . .

TY  - CONF
AU  - Trajković, Milena
AU  - Milošević, Snežana
AU  - Cingel, Aleksandar
AU  - Antonić Reljin, Dragana
AU  - Marković, Marija
AU  - Trifunović-Momčilov, Milana
AU  - Subotić, Angelina
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6782
AB  - Impatiens walleriana is a highly popular ornamental plant worldwide. The major problem in I. walleriana
production, transport and sale display is related to tendency to wilt quickly when drought-stressed thus becoming
undesirable to customers. Salicylic acid (SA) is now considered as an endogenous growth regulator
that influences many plant physiological processes including regulation of plant defense, and plays a significant
role in plant protection against various abiotic stresses. To evaluate the role of exogenously applied SA
against the effect of drought simulated by polyethylene glycol (PEG 8000), growth and physiological parameters
of I. walleriana were analyzed. In vitro developed shoots of I. walleriana were grown on MS media containing
different levels of PEG (0, 1, 2 and 3%) and treated with SA at varying concentrations (0, 1, 2 and 3
mM). Increasing PEG concentrations progressively reduced plant growth parameters (plant height, number
of leaves, fresh weight and proliferation rate) and decreased relative water content, chlorophyll and total pigments
in I. walleriana leaves. In contrast, SA treatments under simulated water deficit conditions enhanced I.
walleriana growth performance. Additionally, a decline in the photosynthetic pigment contents and the level
of water loss in leaves of drought stressed plants were significantly reduced with SA application. Taken together,
these results show that SA improves performance of I. walleriana under stress conditions, suggesting
that exogenous application of SA can be used to increase tolerance of Impatiens plants against water deficit.
PB  - Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade
C3  - Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
T1  - Influence of salicylic acid on morphological and physiological characteristics of Impatiens walleriana L. under water stress induced by polyethylene glycol
SP  - 170
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6782
ER  - 

@conference{
author = "Trajković, Milena and Milošević, Snežana and Cingel, Aleksandar and Antonić Reljin, Dragana and Marković, Marija and Trifunović-Momčilov, Milana and Subotić, Angelina",
year = "2015",
abstract = "Impatiens walleriana is a highly popular ornamental plant worldwide. The major problem in I. walleriana
production, transport and sale display is related to tendency to wilt quickly when drought-stressed thus becoming
undesirable to customers. Salicylic acid (SA) is now considered as an endogenous growth regulator
that influences many plant physiological processes including regulation of plant defense, and plays a significant
role in plant protection against various abiotic stresses. To evaluate the role of exogenously applied SA
against the effect of drought simulated by polyethylene glycol (PEG 8000), growth and physiological parameters
of I. walleriana were analyzed. In vitro developed shoots of I. walleriana were grown on MS media containing
different levels of PEG (0, 1, 2 and 3%) and treated with SA at varying concentrations (0, 1, 2 and 3
mM). Increasing PEG concentrations progressively reduced plant growth parameters (plant height, number
of leaves, fresh weight and proliferation rate) and decreased relative water content, chlorophyll and total pigments
in I. walleriana leaves. In contrast, SA treatments under simulated water deficit conditions enhanced I.
walleriana growth performance. Additionally, a decline in the photosynthetic pigment contents and the level
of water loss in leaves of drought stressed plants were significantly reduced with SA application. Taken together,
these results show that SA improves performance of I. walleriana under stress conditions, suggesting
that exogenous application of SA can be used to increase tolerance of Impatiens plants against water deficit.",
publisher = "Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade",
journal = "Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia",
title = "Influence of salicylic acid on morphological and physiological characteristics of Impatiens walleriana L. under water stress induced by polyethylene glycol",
pages = "170",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6782"
}

Trajković, M., Milošević, S., Cingel, A., Antonić Reljin, D., Marković, M., Trifunović-Momčilov, M.,& Subotić, A.. (2015). Influence of salicylic acid on morphological and physiological characteristics of Impatiens walleriana L. under water stress induced by polyethylene glycol. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade., 170.
https://hdl.handle.net/21.15107/rcub_ibiss_6782

Trajković M, Milošević S, Cingel A, Antonić Reljin D, Marković M, Trifunović-Momčilov M, Subotić A. Influence of salicylic acid on morphological and physiological characteristics of Impatiens walleriana L. under water stress induced by polyethylene glycol. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia. 2015;:170.
https://hdl.handle.net/21.15107/rcub_ibiss_6782 .

Trajković, Milena, Milošević, Snežana, Cingel, Aleksandar, Antonić Reljin, Dragana, Marković, Marija, Trifunović-Momčilov, Milana, Subotić, Angelina, "Influence of salicylic acid on morphological and physiological characteristics of Impatiens walleriana L. under water stress induced by polyethylene glycol" in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia (2015):170,
https://hdl.handle.net/21.15107/rcub_ibiss_6782 .

TY  - CONF
AU  - Antonić Reljin, Dragana
AU  - Marković, Marija
AU  - Simonović, Ana
AU  - Milošević, Snežana
AU  - Trajković, Milena
AU  - Subotić, Angelina
AU  - Jevremović, Slađana
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6780
AB  - Impatiens walleriana is an ornamental plant sensitive to dehydration with tendency to wilt without water.
Proper tissue dehydration is a necessary step in cryopreservation protocols to avoid formation of intracellular
ice crystals which are lethal for plant cells. In order to increase dehydration tolerance of I. walleriana shoots,
we evaluated proline accumulation, a common osmoprotectant, in I. walleriana shoots after two weeks of
culture at several dehydration pretreatments: increased concentration of sucrose (3, 4, 5, 6, and 10%), at different
temperatures (25 ± 2 °C and 4 °C) and different concentration of abscisic acid (ABA, 0, 0.1, 0.2, 0.5, 1, 5
and 10 mg L-1). We observed that graduated concentration of sucrose in culture media increased proline accumulation
in I. walleriana shoots grown under standard conditions, as well as at 4 °C. The highest proline
concentration was recorded in shoots grown on media with 6% sucrose (100 ± 2.3 μmol g-1) at 25 °C which
was more than three times higher than on medium with 3% sucrose (30 ± 0.6 μmol g-1). The highest proline
accumulation in shoots grown at 4 °C was observed at 10% sucrose (88 ± 0.9 μmol g-1) in comparison to
37 ± 0.6 μmol g-1 at 3% sucrose. Addition of ABA in culture medium increased proline concentration in shoots
grown on media with 10 mg L-1 ABA (68 ± 1.2 μmol g-1). Our results suggest that applied pretreatments significantly
increase accumulation of proline and can be helpful in increasing dehydration tolerance of I. walleriana
shoots prior to cryopreservation.
PB  - Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade
C3  - Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
T1  - Proline accumulation in Impatiens walleriana shoots after different dehydration treatments
SP  - 43
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6780
ER  - 

@conference{
author = "Antonić Reljin, Dragana and Marković, Marija and Simonović, Ana and Milošević, Snežana and Trajković, Milena and Subotić, Angelina and Jevremović, Slađana",
year = "2015",
abstract = "Impatiens walleriana is an ornamental plant sensitive to dehydration with tendency to wilt without water.
Proper tissue dehydration is a necessary step in cryopreservation protocols to avoid formation of intracellular
ice crystals which are lethal for plant cells. In order to increase dehydration tolerance of I. walleriana shoots,
we evaluated proline accumulation, a common osmoprotectant, in I. walleriana shoots after two weeks of
culture at several dehydration pretreatments: increased concentration of sucrose (3, 4, 5, 6, and 10%), at different
temperatures (25 ± 2 °C and 4 °C) and different concentration of abscisic acid (ABA, 0, 0.1, 0.2, 0.5, 1, 5
and 10 mg L-1). We observed that graduated concentration of sucrose in culture media increased proline accumulation
in I. walleriana shoots grown under standard conditions, as well as at 4 °C. The highest proline
concentration was recorded in shoots grown on media with 6% sucrose (100 ± 2.3 μmol g-1) at 25 °C which
was more than three times higher than on medium with 3% sucrose (30 ± 0.6 μmol g-1). The highest proline
accumulation in shoots grown at 4 °C was observed at 10% sucrose (88 ± 0.9 μmol g-1) in comparison to
37 ± 0.6 μmol g-1 at 3% sucrose. Addition of ABA in culture medium increased proline concentration in shoots
grown on media with 10 mg L-1 ABA (68 ± 1.2 μmol g-1). Our results suggest that applied pretreatments significantly
increase accumulation of proline and can be helpful in increasing dehydration tolerance of I. walleriana
shoots prior to cryopreservation.",
publisher = "Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade",
journal = "Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia",
title = "Proline accumulation in Impatiens walleriana shoots after different dehydration treatments",
pages = "43",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6780"
}

Antonić Reljin, D., Marković, M., Simonović, A., Milošević, S., Trajković, M., Subotić, A.,& Jevremović, S.. (2015). Proline accumulation in Impatiens walleriana shoots after different dehydration treatments. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade., 43.
https://hdl.handle.net/21.15107/rcub_ibiss_6780

Antonić Reljin D, Marković M, Simonović A, Milošević S, Trajković M, Subotić A, Jevremović S. Proline accumulation in Impatiens walleriana shoots after different dehydration treatments. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia. 2015;:43.
https://hdl.handle.net/21.15107/rcub_ibiss_6780 .

Antonić Reljin, Dragana, Marković, Marija, Simonović, Ana, Milošević, Snežana, Trajković, Milena, Subotić, Angelina, Jevremović, Slađana, "Proline accumulation in Impatiens walleriana shoots after different dehydration treatments" in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia (2015):43,
https://hdl.handle.net/21.15107/rcub_ibiss_6780 .

TY  - CONF
AU  - Trajković, Milena
AU  - Antonić Reljin, Dragana
AU  - Jeknić, Zoran
AU  - Jeknić, Stevan
AU  - Cingel, Aleksandar
AU  - Subotić, Angelina
AU  - Jevremović, Slađana
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6779
AB  - We developed an efficient plant regeneration protocol for a yellow cultivar of Viola cornuta ‘Lutea Splendens’.
Calli and shoots were induced from seedling explants (petiole, leaf, hypocotyls) by one month of culture
on ½-strength MS medium supplemented with 2,4-D (0.1 mg L-1) and BA (2.0 mg L-1). The highest frequency
of shoot induction (12%) was obtained from petiole explants, while the highest frequency of callus formation
(97%) was achieved on hypocotyl explants. The effects of explant origin (top, center, and bottom of hypocotyl)
and light/dark conditions on shoot and callus induction in hypocotyl cultures were also investigated. The
highest shoot induction in hypocotyl cultures (30%) was achieved when explants from the upper section of
the hypocotyls (near the epicotyls) were cultured in light conditions. Shoot multiplication from all explants
was achieved by transferring initially formed shoots to ½strength MS medium supplemented with NAA (0.2
mg L-1), GA3 (2.0 mg L-1), and TDZ (1.0 mg L-1) for four weeks of culture. Developed shoots were then further
multiplied on ½strength MS medium supplemented with NAA (0.5 mg L-1) and BA (1.0 mg L-1). Regenerated
shoots successfully rooted on ½-strength MS medium without growth regulators, acclimatized in a greenhouse
in autumn, and bloomed the following spring. All the regenerated plants exhibited normal morphology
and flower color. This regeneration protocol could be useful for mass propagation using in vitro cultures
and genetic transformation studies using seedling explants.
PB  - Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade
C3  - Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
T1  - Efficient plant regeneration of Viola cornuta ‘Lutea Splendens’ L. using seedling explants
SP  - 25
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6779
ER  - 

@conference{
author = "Trajković, Milena and Antonić Reljin, Dragana and Jeknić, Zoran and Jeknić, Stevan and Cingel, Aleksandar and Subotić, Angelina and Jevremović, Slađana",
year = "2015",
abstract = "We developed an efficient plant regeneration protocol for a yellow cultivar of Viola cornuta ‘Lutea Splendens’.
Calli and shoots were induced from seedling explants (petiole, leaf, hypocotyls) by one month of culture
on ½-strength MS medium supplemented with 2,4-D (0.1 mg L-1) and BA (2.0 mg L-1). The highest frequency
of shoot induction (12%) was obtained from petiole explants, while the highest frequency of callus formation
(97%) was achieved on hypocotyl explants. The effects of explant origin (top, center, and bottom of hypocotyl)
and light/dark conditions on shoot and callus induction in hypocotyl cultures were also investigated. The
highest shoot induction in hypocotyl cultures (30%) was achieved when explants from the upper section of
the hypocotyls (near the epicotyls) were cultured in light conditions. Shoot multiplication from all explants
was achieved by transferring initially formed shoots to ½strength MS medium supplemented with NAA (0.2
mg L-1), GA3 (2.0 mg L-1), and TDZ (1.0 mg L-1) for four weeks of culture. Developed shoots were then further
multiplied on ½strength MS medium supplemented with NAA (0.5 mg L-1) and BA (1.0 mg L-1). Regenerated
shoots successfully rooted on ½-strength MS medium without growth regulators, acclimatized in a greenhouse
in autumn, and bloomed the following spring. All the regenerated plants exhibited normal morphology
and flower color. This regeneration protocol could be useful for mass propagation using in vitro cultures
and genetic transformation studies using seedling explants.",
publisher = "Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade",
journal = "Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia",
title = "Efficient plant regeneration of Viola cornuta ‘Lutea Splendens’ L. using seedling explants",
pages = "25",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6779"
}

Trajković, M., Antonić Reljin, D., Jeknić, Z., Jeknić, S., Cingel, A., Subotić, A.,& Jevremović, S.. (2015). Efficient plant regeneration of Viola cornuta ‘Lutea Splendens’ L. using seedling explants. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade., 25.
https://hdl.handle.net/21.15107/rcub_ibiss_6779

Trajković M, Antonić Reljin D, Jeknić Z, Jeknić S, Cingel A, Subotić A, Jevremović S. Efficient plant regeneration of Viola cornuta ‘Lutea Splendens’ L. using seedling explants. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia. 2015;:25.
https://hdl.handle.net/21.15107/rcub_ibiss_6779 .

Trajković, Milena, Antonić Reljin, Dragana, Jeknić, Zoran, Jeknić, Stevan, Cingel, Aleksandar, Subotić, Angelina, Jevremović, Slađana, "Efficient plant regeneration of Viola cornuta ‘Lutea Splendens’ L. using seedling explants" in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia (2015):25,
https://hdl.handle.net/21.15107/rcub_ibiss_6779 .

TY  - CONF
AU  - Antonić Reljin, Dragana
AU  - Milošević, Snežana
AU  - Trajković, Milena
AU  - Subotić, Angelina
AU  - Jevremović, Slađana
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6781
AB  - Vitrification with plant vitrification solution 2 (PVS2) is commonly used procedure for chemical dehydration
of tissue prior to cryopreservation of the shoot tips. PVS2 contains 30% glycerol (w/v), 15% ethylene glycol
(w/v) and 15% dimethylsulfoxide (DMSO; w/v) in basal culture medium containing 0.4 M sucrose. In order
to develop PVS2-vitrification based protocol for cryopreservation of Impatiens walleriana shoot tips, we tested
the effect of different PVS2 concentrations (100, 80, 60, 50, and 40%), and time of shoot tips exposure to
PVS2 solution (5, 10, 15, 20, 25 and 30 minutes) on survival and plant regeneration prior and after exposure
to liquid nitrogen (LN). Nodal segments of I. walleriana were cultured on plant growth regulator-free MS medium
for three weeks before the shoot tips were isolated. The results have shown that survival of shoot tips
prior to exposure to liquid nitrogen depended on PVS2 concentration and time of exposure to PVS2 solution
and ranged from 100% (lower PVS2 concentrations) to 50% (100% of PVS2, 30 min exposure). After exposure
to LN, the highest survival (77%) was achieved when shoot tips were exposed 25 min to 50% PVS2 solution,
while full strength PVS2 solution with 30 min exposure was harmful when only 26% of cultured shoot tips
survived. The highest plant regeneration (10.3%) of the cryopreserved shoot tips was observed when shoot
tips were exposed 25 minutes to 50% PVS2 solution prior to immersing in LN. Viability of PVS2 treated shoot
tips was determined by staining with fluorescein diacetate.
PB  - Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade
C3  - Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
T1  - Cryopreservation of Impatiens walleriana shoot tips using PVS2 vitrification procedure
SP  - 119
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6781
ER  - 

@conference{
author = "Antonić Reljin, Dragana and Milošević, Snežana and Trajković, Milena and Subotić, Angelina and Jevremović, Slađana",
year = "2015",
abstract = "Vitrification with plant vitrification solution 2 (PVS2) is commonly used procedure for chemical dehydration
of tissue prior to cryopreservation of the shoot tips. PVS2 contains 30% glycerol (w/v), 15% ethylene glycol
(w/v) and 15% dimethylsulfoxide (DMSO; w/v) in basal culture medium containing 0.4 M sucrose. In order
to develop PVS2-vitrification based protocol for cryopreservation of Impatiens walleriana shoot tips, we tested
the effect of different PVS2 concentrations (100, 80, 60, 50, and 40%), and time of shoot tips exposure to
PVS2 solution (5, 10, 15, 20, 25 and 30 minutes) on survival and plant regeneration prior and after exposure
to liquid nitrogen (LN). Nodal segments of I. walleriana were cultured on plant growth regulator-free MS medium
for three weeks before the shoot tips were isolated. The results have shown that survival of shoot tips
prior to exposure to liquid nitrogen depended on PVS2 concentration and time of exposure to PVS2 solution
and ranged from 100% (lower PVS2 concentrations) to 50% (100% of PVS2, 30 min exposure). After exposure
to LN, the highest survival (77%) was achieved when shoot tips were exposed 25 min to 50% PVS2 solution,
while full strength PVS2 solution with 30 min exposure was harmful when only 26% of cultured shoot tips
survived. The highest plant regeneration (10.3%) of the cryopreserved shoot tips was observed when shoot
tips were exposed 25 minutes to 50% PVS2 solution prior to immersing in LN. Viability of PVS2 treated shoot
tips was determined by staining with fluorescein diacetate.",
publisher = "Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade",
journal = "Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia",
title = "Cryopreservation of Impatiens walleriana shoot tips using PVS2 vitrification procedure",
pages = "119",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6781"
}

Antonić Reljin, D., Milošević, S., Trajković, M., Subotić, A.,& Jevremović, S.. (2015). Cryopreservation of Impatiens walleriana shoot tips using PVS2 vitrification procedure. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade., 119.
https://hdl.handle.net/21.15107/rcub_ibiss_6781

Antonić Reljin D, Milošević S, Trajković M, Subotić A, Jevremović S. Cryopreservation of Impatiens walleriana shoot tips using PVS2 vitrification procedure. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia. 2015;:119.
https://hdl.handle.net/21.15107/rcub_ibiss_6781 .

Antonić Reljin, Dragana, Milošević, Snežana, Trajković, Milena, Subotić, Angelina, Jevremović, Slađana, "Cryopreservation of Impatiens walleriana shoot tips using PVS2 vitrification procedure" in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia (2015):119,
https://hdl.handle.net/21.15107/rcub_ibiss_6781 .

TY  - CONF
AU  - Jeknić, Stevan
AU  - Jevremović, Slađana
AU  - Trajković, Milena
AU  - Subotić, Angelina
AU  - Jeknić, Zoran
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6778
AB  - The elusive red iris (I. germanica, I. hollandica) has been a subject of interest for over a century as it does
not exist naturally and efforts with classical breeding methods have failed to produce it. Genetic engineering,
on the other hand, offers alternative ways of introducing this desirable trait by expanding the color-determining
gene pool that is available. We first studied the effects of ectopic expression of a bacterial phytoene synthase
gene (crtB) from Pantoea agglomerans in the pink I. germanica cultivar ‘Fire Bride’. This approach aimed
to increase the flux of metabolites into the carotenoid biosynthetic pathway, and ultimately lead to elevated
accumulation of lycopene, thus generating darker pink or red flowers. CrtB-transgenic plants showed prominent
color changes in the ovaries (green to orange), flower stalk (green to orange), and anthers (white to
pink) when compared to control plants, while the standards and falls showed no significant color change.
Next, we studied the feasibility of using a gene for capsanthin-capsorubin synthase (Llccs) from tiger lily (Lilium
lancifolium ‘Splendens’) in yellow iris flowers to alter their color to red. This approach aimed to produce
the flower-specific accumulation of two red κ-xanthophylls, capsanthin and capsorubin. Llccs-transgenic iriscalli successfully accumulated the novel carotenoids and changed color from yellow to red-orange, but no
plants were regenerated due to the lack of morphogenic response from the cultivar ‘Hot Property’. Efforts are
currently under way to transform different morphogenic yellow cultivars with Llccs to produce red I. germanica,
as well as I. hollandica, flowers.
PB  - Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade
C3  - Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
T1  - Recent advancements in the production of a red Iris flower through genetic engineering
SP  - 23
EP  - 24
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6778
ER  - 

@conference{
author = "Jeknić, Stevan and Jevremović, Slađana and Trajković, Milena and Subotić, Angelina and Jeknić, Zoran",
year = "2015",
abstract = "The elusive red iris (I. germanica, I. hollandica) has been a subject of interest for over a century as it does
not exist naturally and efforts with classical breeding methods have failed to produce it. Genetic engineering,
on the other hand, offers alternative ways of introducing this desirable trait by expanding the color-determining
gene pool that is available. We first studied the effects of ectopic expression of a bacterial phytoene synthase
gene (crtB) from Pantoea agglomerans in the pink I. germanica cultivar ‘Fire Bride’. This approach aimed
to increase the flux of metabolites into the carotenoid biosynthetic pathway, and ultimately lead to elevated
accumulation of lycopene, thus generating darker pink or red flowers. CrtB-transgenic plants showed prominent
color changes in the ovaries (green to orange), flower stalk (green to orange), and anthers (white to
pink) when compared to control plants, while the standards and falls showed no significant color change.
Next, we studied the feasibility of using a gene for capsanthin-capsorubin synthase (Llccs) from tiger lily (Lilium
lancifolium ‘Splendens’) in yellow iris flowers to alter their color to red. This approach aimed to produce
the flower-specific accumulation of two red κ-xanthophylls, capsanthin and capsorubin. Llccs-transgenic iriscalli successfully accumulated the novel carotenoids and changed color from yellow to red-orange, but no
plants were regenerated due to the lack of morphogenic response from the cultivar ‘Hot Property’. Efforts are
currently under way to transform different morphogenic yellow cultivars with Llccs to produce red I. germanica,
as well as I. hollandica, flowers.",
publisher = "Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade",
journal = "Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia",
title = "Recent advancements in the production of a red Iris flower through genetic engineering",
pages = "23-24",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6778"
}

Jeknić, S., Jevremović, S., Trajković, M., Subotić, A.,& Jeknić, Z.. (2015). Recent advancements in the production of a red Iris flower through genetic engineering. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
Belgrade: Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade., 23-24.
https://hdl.handle.net/21.15107/rcub_ibiss_6778

Jeknić S, Jevremović S, Trajković M, Subotić A, Jeknić Z. Recent advancements in the production of a red Iris flower through genetic engineering. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia. 2015;:23-24.
https://hdl.handle.net/21.15107/rcub_ibiss_6778 .

Jeknić, Stevan, Jevremović, Slađana, Trajković, Milena, Subotić, Angelina, Jeknić, Zoran, "Recent advancements in the production of a red Iris flower through genetic engineering" in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia (2015):23-24,
https://hdl.handle.net/21.15107/rcub_ibiss_6778 .

TY  - JOUR
AU  - Jevremović, Slađana
AU  - Trajković, Milena
AU  - Jeknić, Zoran
AU  - Trifunović-Momčilov, Milana
AU  - Antonić Reljin, Dragana
AU  - Marković, Marija
AU  - Subotić, Angelina
AU  - Radojević, Ljiljana
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6411
AB  - An efficient propagation protocol by somatic embryogenesis and organogenesis of Balkan endemic iris, Iris reichenbachii, was achieved and clonal fidelity of regenerated plants evaluated. Both regeneration pathways were induced at the same time in zygotic embryo culture on Murashige & Skoog (MS) medium supplemented with 0.5-5.0 mM 2,4-dichlorophenoxy acetic acid (2,4-D) as the sole hormone. Embryogenic calli were further mantained on medium supplemented with 2,4-D and kinetin (Kn; 0.5 and 5.0 mM, respectively). Organogenic calli were selected and further cultured on MS media supplemented with 1-naphthaleneacetic acid (NAA) and benzyladenine (BA; 0.5 and 4.5 mM, respectively) for shoot initiation. Somatic embryos germinated and shoots rooted on MS plant growth regulator-free medium. Plants regenerated by both procceses were succesfully acclimatized in greenhouse conditions and flowered in the following flowering season. Some alterations in flower morphology were detected among plants regenerated by organogenesis. Flow cytometric analysis revealed that plants with altered morphology of flowers had the same ploidy level and genome size as plants collected from the natural habitat. A tetraploid plant was observed in the population of plants regenerated by somatic embryogenesis induced at a high concentration of 2,4-D (10.0 mM).
AB  - Prikazan je efikasan protokol za propagaciju somatskom embriogenezom i organogenezom Balkanske endemične perunike, Iris reichenbachii i procenjena je klonalna indentičnost dobijenih biljaka. Oba načina za regeneraciju u uslovima in vitro su postignuta u kulturi zrelih zigotskih embriona na Murashige & Skoog (MS) hranljivoj podlozi obogaćenoj sa 2,4-dihlorofenoksi sirćetnom kiselinom 0.5-5.0 mM (2,4-D) kao jedinim regulatorom rastenja. Dobijeni embriogeni kalusi su dalje gajeni na hranljivoj podlozi sa 2,4-D i kinetinom (Kn; 0.5 odnosno 5.0 mM). Formirani organogeni kalusi su dalje gajeni na MS hranljivoj podlozi obogaćenoj sa α-naftilsirćetnom kiselinom (NAA) i benziladeninom (BA; 0.5 odnosno 4.5 mM) kada je došlo do formiranja izdanaka. Klijanje somatskih embriona kao i ožiljavanje formiranih izdanaka postignuto je na MS hranljivoj podlozi bez biljnih regulatora rastenja. Biljke dobijene na oba načina su dalje uspešno aklimatizovane na uslove gajenja u stakleniku i cvetale su sledeće godine u proleće. Uočene su neke promene u morfologiji cvetova kod biljaka dobijenih procesom organogeneze. Na osnovu flow-citometrijske analize pokazano je da su biljke sa izmenjenom morfologijom cvetova imale isti nivo ploidnosti i veličinu genoma kao biljke iz prirode. U populaciji biljaka regenerisanih procesom somatske embriogeneze koja je indukovana na podlozi sa visokom koncentracijom 2,4-D (10.0 mM) jedna biljka je bila tetraploidna.
PB  - Belgrade: Institute of Botany and Botanical Garden Jevremovac
T2  - Botanica Serbica
T1  - In vitro propagation of Iris reichenbachii Heuff. and clonal fidelity of regenerated plants
T1  - In vitro propagacija Iris reichenbachii Heuff. i klonalna identičnost dobijenih biljaka
IS  - 2
VL  - 39
SP  - 129
EP  - 136
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6411
ER  - 

@article{
author = "Jevremović, Slađana and Trajković, Milena and Jeknić, Zoran and Trifunović-Momčilov, Milana and Antonić Reljin, Dragana and Marković, Marija and Subotić, Angelina and Radojević, Ljiljana",
year = "2015",
abstract = "An efficient propagation protocol by somatic embryogenesis and organogenesis of Balkan endemic iris, Iris reichenbachii, was achieved and clonal fidelity of regenerated plants evaluated. Both regeneration pathways were induced at the same time in zygotic embryo culture on Murashige & Skoog (MS) medium supplemented with 0.5-5.0 mM 2,4-dichlorophenoxy acetic acid (2,4-D) as the sole hormone. Embryogenic calli were further mantained on medium supplemented with 2,4-D and kinetin (Kn; 0.5 and 5.0 mM, respectively). Organogenic calli were selected and further cultured on MS media supplemented with 1-naphthaleneacetic acid (NAA) and benzyladenine (BA; 0.5 and 4.5 mM, respectively) for shoot initiation. Somatic embryos germinated and shoots rooted on MS plant growth regulator-free medium. Plants regenerated by both procceses were succesfully acclimatized in greenhouse conditions and flowered in the following flowering season. Some alterations in flower morphology were detected among plants regenerated by organogenesis. Flow cytometric analysis revealed that plants with altered morphology of flowers had the same ploidy level and genome size as plants collected from the natural habitat. A tetraploid plant was observed in the population of plants regenerated by somatic embryogenesis induced at a high concentration of 2,4-D (10.0 mM)., Prikazan je efikasan protokol za propagaciju somatskom embriogenezom i organogenezom Balkanske endemične perunike, Iris reichenbachii i procenjena je klonalna indentičnost dobijenih biljaka. Oba načina za regeneraciju u uslovima in vitro su postignuta u kulturi zrelih zigotskih embriona na Murashige & Skoog (MS) hranljivoj podlozi obogaćenoj sa 2,4-dihlorofenoksi sirćetnom kiselinom 0.5-5.0 mM (2,4-D) kao jedinim regulatorom rastenja. Dobijeni embriogeni kalusi su dalje gajeni na hranljivoj podlozi sa 2,4-D i kinetinom (Kn; 0.5 odnosno 5.0 mM). Formirani organogeni kalusi su dalje gajeni na MS hranljivoj podlozi obogaćenoj sa α-naftilsirćetnom kiselinom (NAA) i benziladeninom (BA; 0.5 odnosno 4.5 mM) kada je došlo do formiranja izdanaka. Klijanje somatskih embriona kao i ožiljavanje formiranih izdanaka postignuto je na MS hranljivoj podlozi bez biljnih regulatora rastenja. Biljke dobijene na oba načina su dalje uspešno aklimatizovane na uslove gajenja u stakleniku i cvetale su sledeće godine u proleće. Uočene su neke promene u morfologiji cvetova kod biljaka dobijenih procesom organogeneze. Na osnovu flow-citometrijske analize pokazano je da su biljke sa izmenjenom morfologijom cvetova imale isti nivo ploidnosti i veličinu genoma kao biljke iz prirode. U populaciji biljaka regenerisanih procesom somatske embriogeneze koja je indukovana na podlozi sa visokom koncentracijom 2,4-D (10.0 mM) jedna biljka je bila tetraploidna.",
publisher = "Belgrade: Institute of Botany and Botanical Garden Jevremovac",
journal = "Botanica Serbica",
title = "In vitro propagation of Iris reichenbachii Heuff. and clonal fidelity of regenerated plants, In vitro propagacija Iris reichenbachii Heuff. i klonalna identičnost dobijenih biljaka",
number = "2",
volume = "39",
pages = "129-136",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6411"
}

Jevremović, S., Trajković, M., Jeknić, Z., Trifunović-Momčilov, M., Antonić Reljin, D., Marković, M., Subotić, A.,& Radojević, L.. (2015). In vitro propagation of Iris reichenbachii Heuff. and clonal fidelity of regenerated plants. in Botanica Serbica
Belgrade: Institute of Botany and Botanical Garden Jevremovac., 39(2), 129-136.
https://hdl.handle.net/21.15107/rcub_ibiss_6411

Jevremović S, Trajković M, Jeknić Z, Trifunović-Momčilov M, Antonić Reljin D, Marković M, Subotić A, Radojević L. In vitro propagation of Iris reichenbachii Heuff. and clonal fidelity of regenerated plants. in Botanica Serbica. 2015;39(2):129-136.
https://hdl.handle.net/21.15107/rcub_ibiss_6411 .

Jevremović, Slađana, Trajković, Milena, Jeknić, Zoran, Trifunović-Momčilov, Milana, Antonić Reljin, Dragana, Marković, Marija, Subotić, Angelina, Radojević, Ljiljana, "In vitro propagation of Iris reichenbachii Heuff. and clonal fidelity of regenerated plants" in Botanica Serbica, 39, no. 2 (2015):129-136,
https://hdl.handle.net/21.15107/rcub_ibiss_6411 .

TY  - JOUR
AU  - Trajković, Milena
AU  - Vinterhalter, Branka
AU  - Subotić, Angelina
AU  - Vinterhalter, Dragan
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6412
AB  - The regenerative potential of in vitro-produced bulblets was investigated in three commercial Oriental lily cultivars (‘Aubade’, ‘Belcanto’ and ‘Solaia’) in relation to two synthetic cytokinins  BA and TDZ and picloram as representative of auxins. Single bulblet leaflets were excised and cultured on MS medium supplemented with either 6-benzyladenine (BA 0-2.0 mg/l), thidiazurone (TDZ 0-2.0 mg/l) or picloram (PIC 0-3.0 mg/l). In all three cultivars and medium combinations explants after 5 weeks regenerated somatic embryos, bulblets and plantlets. While bulblet production was balanced, plantlet and somatic embryogenesis (SE) production were complementary with pronounced SE production at higher plant growth regulator concentrations and plantlet production at lower concentrations. Picloram had a sharp regeneration demarcation with low plantlet production above 0.5mg/l. BA and TDZ produced SE at all concentrations including hormone-free controls. On media with TDZ and BA there was a gradual change from bulblet regeneration at lower to somatic embryogenesis at higher concentrations. For all three cultivars, details of the regeneration process were studied by histological techniques in TDZ-supplemented medium, showing early stage SE regeneration in all samples. Mature, elongated SE stages were missing, indicating early transition of SE into bulblets. The optimal propagation conditions were elaborated for all three lily cultivars.
AB  - Regenerativni potencijal in vitro razmnoženih lukovica istražen je kod tri komercijalne sorte Orijental ljiljana (‘Aubade’, ‘Belcanto’ and ‘Solaia’) u odnosu na dva sintetička citokinina BAP i  TDZ kao i na pikloram regulator rastenja sa auksinskom aktivnošću. Listići sa in vitro lukovica su izolovani i kultivisani na MS podlogama sa 6-benzil aminopurinom (BAP 0-2.0 mg/l), thidiazuronom (TDZ 0-2.0 mg/l) ili pikloramom (PIC 0-3.0 mg/l). Kod sve tri sorte i u svim kombinacijama podloga eksplantati su nakon 5 nedelja regenerisali somatske embrione, lukovičice ili biljke. Dok je produkcija lukovičica bila izbalansirana produkcija biljaka i produkcija ranih somatskih embriona bile su komplementarne sa izrazitom produkcijom SE na višim koncentracijama regulatora rastenja i produkcijom biljaka na nižim koncentracijama. Pikloram je pokazivao jasnu regeneracionu demarkaciju sa niskom produkcijom biljaka na podlogama sa ili iznad 0.5 mg/l. BAP i TDZ su produkovali somatske embrione na svim koncentracijama regulatora rastenja uključujući i podloge bez regulatora rastenja. Podloge sa  TDZ i BAP pokazivale su postepenu promenu od regeneracije lukovica na nižim prema SE na višim koncentracijama. Kod sva tri kultivara proces regeneracije praćen je histološki na podlogama sa TDZ i pokazano je prisustvo direktne SE. Stariji, izduženi stupnjevi SE su bili odsutni što ukazuje na ranu tranziciju somatskih embriona ljiljana u lukovičice. Optimalni uslovi za razmnožavanje razradjeni su i prikazani za sva tri sorte ljiljana.
PB  - Belgrade: Institute of Botany and Botanical Garden Jevremovac
T2  - Botanica Serbica
T1  - Differences in regenerative capacity of Oriental lily (Lilium sp.) cultivars
T1  - Različiti regenerativni potencijal kultivara orjentalnog ljiljana (Lilium sp.)
IS  - 2
VL  - 39
SP  - 159
EP  - 167
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6412
ER  - 

@article{
author = "Trajković, Milena and Vinterhalter, Branka and Subotić, Angelina and Vinterhalter, Dragan",
year = "2015",
abstract = "The regenerative potential of in vitro-produced bulblets was investigated in three commercial Oriental lily cultivars (‘Aubade’, ‘Belcanto’ and ‘Solaia’) in relation to two synthetic cytokinins  BA and TDZ and picloram as representative of auxins. Single bulblet leaflets were excised and cultured on MS medium supplemented with either 6-benzyladenine (BA 0-2.0 mg/l), thidiazurone (TDZ 0-2.0 mg/l) or picloram (PIC 0-3.0 mg/l). In all three cultivars and medium combinations explants after 5 weeks regenerated somatic embryos, bulblets and plantlets. While bulblet production was balanced, plantlet and somatic embryogenesis (SE) production were complementary with pronounced SE production at higher plant growth regulator concentrations and plantlet production at lower concentrations. Picloram had a sharp regeneration demarcation with low plantlet production above 0.5mg/l. BA and TDZ produced SE at all concentrations including hormone-free controls. On media with TDZ and BA there was a gradual change from bulblet regeneration at lower to somatic embryogenesis at higher concentrations. For all three cultivars, details of the regeneration process were studied by histological techniques in TDZ-supplemented medium, showing early stage SE regeneration in all samples. Mature, elongated SE stages were missing, indicating early transition of SE into bulblets. The optimal propagation conditions were elaborated for all three lily cultivars., Regenerativni potencijal in vitro razmnoženih lukovica istražen je kod tri komercijalne sorte Orijental ljiljana (‘Aubade’, ‘Belcanto’ and ‘Solaia’) u odnosu na dva sintetička citokinina BAP i  TDZ kao i na pikloram regulator rastenja sa auksinskom aktivnošću. Listići sa in vitro lukovica su izolovani i kultivisani na MS podlogama sa 6-benzil aminopurinom (BAP 0-2.0 mg/l), thidiazuronom (TDZ 0-2.0 mg/l) ili pikloramom (PIC 0-3.0 mg/l). Kod sve tri sorte i u svim kombinacijama podloga eksplantati su nakon 5 nedelja regenerisali somatske embrione, lukovičice ili biljke. Dok je produkcija lukovičica bila izbalansirana produkcija biljaka i produkcija ranih somatskih embriona bile su komplementarne sa izrazitom produkcijom SE na višim koncentracijama regulatora rastenja i produkcijom biljaka na nižim koncentracijama. Pikloram je pokazivao jasnu regeneracionu demarkaciju sa niskom produkcijom biljaka na podlogama sa ili iznad 0.5 mg/l. BAP i TDZ su produkovali somatske embrione na svim koncentracijama regulatora rastenja uključujući i podloge bez regulatora rastenja. Podloge sa  TDZ i BAP pokazivale su postepenu promenu od regeneracije lukovica na nižim prema SE na višim koncentracijama. Kod sva tri kultivara proces regeneracije praćen je histološki na podlogama sa TDZ i pokazano je prisustvo direktne SE. Stariji, izduženi stupnjevi SE su bili odsutni što ukazuje na ranu tranziciju somatskih embriona ljiljana u lukovičice. Optimalni uslovi za razmnožavanje razradjeni su i prikazani za sva tri sorte ljiljana.",
publisher = "Belgrade: Institute of Botany and Botanical Garden Jevremovac",
journal = "Botanica Serbica",
title = "Differences in regenerative capacity of Oriental lily (Lilium sp.) cultivars, Različiti regenerativni potencijal kultivara orjentalnog ljiljana (Lilium sp.)",
number = "2",
volume = "39",
pages = "159-167",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6412"
}

Trajković, M., Vinterhalter, B., Subotić, A.,& Vinterhalter, D.. (2015). Differences in regenerative capacity of Oriental lily (Lilium sp.) cultivars. in Botanica Serbica
Belgrade: Institute of Botany and Botanical Garden Jevremovac., 39(2), 159-167.
https://hdl.handle.net/21.15107/rcub_ibiss_6412

Trajković M, Vinterhalter B, Subotić A, Vinterhalter D. Differences in regenerative capacity of Oriental lily (Lilium sp.) cultivars. in Botanica Serbica. 2015;39(2):159-167.
https://hdl.handle.net/21.15107/rcub_ibiss_6412 .

Trajković, Milena, Vinterhalter, Branka, Subotić, Angelina, Vinterhalter, Dragan, "Differences in regenerative capacity of Oriental lily (Lilium sp.) cultivars" in Botanica Serbica, 39, no. 2 (2015):159-167,
https://hdl.handle.net/21.15107/rcub_ibiss_6412 .

TY  - JOUR
AU  - Milošević, Snežana
AU  - Trajković, Milena
AU  - Antonić Reljin, Dragana
AU  - Cingel, Aleksandar
AU  - Subotić, Angelina
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6210
AB  - Impatiens walleriana L. shoots were inoculated with Agrobacterium rhizogenes
A4M70GUS and the effects of genetic transformation on the catalase (CAT), superoxide
dismutase (SOD) and peroxidase (POX) activities in wounded region of stems and
unwounded leaves were evaluated 10, 24, 240 and 720 hours after inoculation. Following
Agrobacterum infection activities of plant antioxidative enzymes changed in a timedependent manner indicating that dynamic processes occurred during plantAgrobacterium interaction, plant cell transformation and formation of hairy roots.
Appearance of hairy roots on wound sites of shoots was observed ten days after
inoculation with A. rhizogenes and the root induction frequency was 100%. Among
selected hairy root lines significant differences in growth rate and biomass production
were observed and an average 3-fold increase in biomass production was observed for the
best growing hairy root line compared with the untransformed roots. PCR analysis
showed presence of uidA, rolB, rolC and rolD genes in all analyzed I. walleriana L. hairy
root lines, while amplification fragment of rolA gene was detected in 83.3% transformed
lines. Efficient transformation protocol for I. walleriana L described in this work offer
possibilities to generate hairy root cultures for in vitro propagation of plant viruses.
AB  - Izdanci Impatiens walleriana L. su inokulisani sa Agrobacterium rhizogenes A4M70GUS i praćen
je efekat genetičke transformacije na akivnost katalaza (CAT), superoksid dismutaza (SOD) i
peroksidaza (POX) u regionu povrede izdanka kao i u nepovređenim listovima 10, 24, 240 i 720
sati nakon infekcije. Promene u aktivnosti antioksidativnih enzima biljke u zavisnosti od vremena
nakon infekcije ukazuju na dinamične procese koji se odigravaju tokom interakcije biljke i
Agrobakterije, transformacije biljnih ćelija i formiranja “hairy roots” (transformisanih korenova).
Pojava transformisanih korenova je uočena 10 dana nakon inokulacije i učestalost njihove
indukcije iznosila je 100%. Između izabranih “hairy roots” linija postojale su značajne razlike u
stopi rasta i uvećanju biomase, a za transformisanu liniju korenova koja je pokazivala najbolji rast
zabeležen je trostruko veći porast biomase u odnosu na netransformisane korenove. PCR analizom
je potvrđeno prisustvo uidA, rolB, rolC and rolD gena u svim analiziranim linijama
transformisanih korenova I. walleriana L. dok je fragment amplifikacije rolA gena detektovan kod
83.3% transformisanih linija. Efikasni protokol transformacije I. walleriana L koji je opisan u
ovom radu pruža mogućnost stvaranja kultura transformisanih korenova u cilju in vitro
propagacije biljnih virusa.
PB  - Belgrade: Serbian Genetics Society
T2  - Genetika
T1  - Changes of antioxidative enzymes in Impatiens walleriana L. shoots in response to genetic transformation
T1  - Promene antioksidativnih enzima u izdancima Impatiens walleriana L. kao odgovor na genetičku transformaciju
IS  - 1
VL  - 47
DO  - 10.2298/GENSR1501071M
SP  - 71
EP  - 84
ER  - 

@article{
author = "Milošević, Snežana and Trajković, Milena and Antonić Reljin, Dragana and Cingel, Aleksandar and Subotić, Angelina",
year = "2015",
abstract = "Impatiens walleriana L. shoots were inoculated with Agrobacterium rhizogenes
A4M70GUS and the effects of genetic transformation on the catalase (CAT), superoxide
dismutase (SOD) and peroxidase (POX) activities in wounded region of stems and
unwounded leaves were evaluated 10, 24, 240 and 720 hours after inoculation. Following
Agrobacterum infection activities of plant antioxidative enzymes changed in a timedependent manner indicating that dynamic processes occurred during plantAgrobacterium interaction, plant cell transformation and formation of hairy roots.
Appearance of hairy roots on wound sites of shoots was observed ten days after
inoculation with A. rhizogenes and the root induction frequency was 100%. Among
selected hairy root lines significant differences in growth rate and biomass production
were observed and an average 3-fold increase in biomass production was observed for the
best growing hairy root line compared with the untransformed roots. PCR analysis
showed presence of uidA, rolB, rolC and rolD genes in all analyzed I. walleriana L. hairy
root lines, while amplification fragment of rolA gene was detected in 83.3% transformed
lines. Efficient transformation protocol for I. walleriana L described in this work offer
possibilities to generate hairy root cultures for in vitro propagation of plant viruses., Izdanci Impatiens walleriana L. su inokulisani sa Agrobacterium rhizogenes A4M70GUS i praćen
je efekat genetičke transformacije na akivnost katalaza (CAT), superoksid dismutaza (SOD) i
peroksidaza (POX) u regionu povrede izdanka kao i u nepovređenim listovima 10, 24, 240 i 720
sati nakon infekcije. Promene u aktivnosti antioksidativnih enzima biljke u zavisnosti od vremena
nakon infekcije ukazuju na dinamične procese koji se odigravaju tokom interakcije biljke i
Agrobakterije, transformacije biljnih ćelija i formiranja “hairy roots” (transformisanih korenova).
Pojava transformisanih korenova je uočena 10 dana nakon inokulacije i učestalost njihove
indukcije iznosila je 100%. Između izabranih “hairy roots” linija postojale su značajne razlike u
stopi rasta i uvećanju biomase, a za transformisanu liniju korenova koja je pokazivala najbolji rast
zabeležen je trostruko veći porast biomase u odnosu na netransformisane korenove. PCR analizom
je potvrđeno prisustvo uidA, rolB, rolC and rolD gena u svim analiziranim linijama
transformisanih korenova I. walleriana L. dok je fragment amplifikacije rolA gena detektovan kod
83.3% transformisanih linija. Efikasni protokol transformacije I. walleriana L koji je opisan u
ovom radu pruža mogućnost stvaranja kultura transformisanih korenova u cilju in vitro
propagacije biljnih virusa.",
publisher = "Belgrade: Serbian Genetics Society",
journal = "Genetika",
title = "Changes of antioxidative enzymes in Impatiens walleriana L. shoots in response to genetic transformation, Promene antioksidativnih enzima u izdancima Impatiens walleriana L. kao odgovor na genetičku transformaciju",
number = "1",
volume = "47",
doi = "10.2298/GENSR1501071M",
pages = "71-84"
}

Milošević, S., Trajković, M., Antonić Reljin, D., Cingel, A.,& Subotić, A.. (2015). Changes of antioxidative enzymes in Impatiens walleriana L. shoots in response to genetic transformation. in Genetika
Belgrade: Serbian Genetics Society., 47(1), 71-84.
https://doi.org/10.2298/GENSR1501071M

Milošević S, Trajković M, Antonić Reljin D, Cingel A, Subotić A. Changes of antioxidative enzymes in Impatiens walleriana L. shoots in response to genetic transformation. in Genetika. 2015;47(1):71-84.
doi:10.2298/GENSR1501071M .

Milošević, Snežana, Trajković, Milena, Antonić Reljin, Dragana, Cingel, Aleksandar, Subotić, Angelina, "Changes of antioxidative enzymes in Impatiens walleriana L. shoots in response to genetic transformation" in Genetika, 47, no. 1 (2015):71-84,
https://doi.org/10.2298/GENSR1501071M . .