TY  - JOUR
AU  - Ajdžanović, Vladimir
AU  - Miler, Marko
AU  - Šošić-Jurjević, Branka
AU  - Filipović, Branko
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6555
AB  - Time perception is an important and to some extent adjustable aspect of the human cognitive sphere. We hypothesize that the specific food consumption can affect the perception of time in two ways, namely through the emotions it triggers and through the nutrients it contains, which may affect brain chemistry. The “internal clock”, responsible for timing tasks, can be charged (accelerated) by arousal caused by visual food stimuli or uncharged (slowed down) in the same context by distraction of attention, leading to overestimation or underestimation of time, respectively. Nutrients can affect the “internal clock” rate in terms of increasing or decreasing it, which affects time estimation in the same way. Animal studies indicate that a protein meal, rich in the amino acid tyrosine, can increase the release of dopamine in the brain and speed up the “internal clock”. Furthermore, a carbohydrate meal can change the balance of amino acids in the brain in favor of tryptophan, which in turn can increase the release of serotonin and slow down the “internal clock”. Given that the modern, fast-paced lifestyle requires the slowing down of subjective time, it seems that the consumption of foods that charge/accelerate the “internal clock” is desirable for this purpose.
PB  - Elsevier Ltd.
T2  - Medical Hypotheses
T1  - Can food affect the perception of time?
VL  - 184
DO  - 10.1016/j.mehy.2024.111283
SP  - 111283
ER  - 

@article{
author = "Ajdžanović, Vladimir and Miler, Marko and Šošić-Jurjević, Branka and Filipović, Branko",
year = "2024",
abstract = "Time perception is an important and to some extent adjustable aspect of the human cognitive sphere. We hypothesize that the specific food consumption can affect the perception of time in two ways, namely through the emotions it triggers and through the nutrients it contains, which may affect brain chemistry. The “internal clock”, responsible for timing tasks, can be charged (accelerated) by arousal caused by visual food stimuli or uncharged (slowed down) in the same context by distraction of attention, leading to overestimation or underestimation of time, respectively. Nutrients can affect the “internal clock” rate in terms of increasing or decreasing it, which affects time estimation in the same way. Animal studies indicate that a protein meal, rich in the amino acid tyrosine, can increase the release of dopamine in the brain and speed up the “internal clock”. Furthermore, a carbohydrate meal can change the balance of amino acids in the brain in favor of tryptophan, which in turn can increase the release of serotonin and slow down the “internal clock”. Given that the modern, fast-paced lifestyle requires the slowing down of subjective time, it seems that the consumption of foods that charge/accelerate the “internal clock” is desirable for this purpose.",
publisher = "Elsevier Ltd.",
journal = "Medical Hypotheses",
title = "Can food affect the perception of time?",
volume = "184",
doi = "10.1016/j.mehy.2024.111283",
pages = "111283"
}

Ajdžanović, V., Miler, M., Šošić-Jurjević, B.,& Filipović, B.. (2024). Can food affect the perception of time?. in Medical Hypotheses
Elsevier Ltd.., 184, 111283.
https://doi.org/10.1016/j.mehy.2024.111283

Ajdžanović V, Miler M, Šošić-Jurjević B, Filipović B. Can food affect the perception of time?. in Medical Hypotheses. 2024;184:111283.
doi:10.1016/j.mehy.2024.111283 .

Ajdžanović, Vladimir, Miler, Marko, Šošić-Jurjević, Branka, Filipović, Branko, "Can food affect the perception of time?" in Medical Hypotheses, 184 (2024):111283,
https://doi.org/10.1016/j.mehy.2024.111283 . .

TY  - JOUR
AU  - Ajdžanović, Vladimir
AU  - Miler, Marko
AU  - Šošić-Jurjević, Branka
AU  - Filipović, Branko
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6554
AB  - Time perception is an important and to some extent adjustable aspect of the human cognitive sphere. We hypothesize that the specific food consumption can affect the perception of time in two ways, namely through the emotions it triggers and through the nutrients it contains, which may affect brain chemistry. The “internal clock”, responsible for timing tasks, can be charged (accelerated) by arousal caused by visual food stimuli or uncharged (slowed down) in the same context by distraction of attention, leading to overestimation or underestimation of time, respectively. Nutrients can affect the “internal clock” rate in terms of increasing or decreasing it, which affects time estimation in the same way. Animal studies indicate that a protein meal, rich in the amino acid tyrosine, can increase the release of dopamine in the brain and speed up the “internal clock”. Furthermore, a carbohydrate meal can change the balance of amino acids in the brain in favor of tryptophan, which in turn can increase the release of serotonin and slow down the “internal clock”. Given that the modern, fast-paced lifestyle requires the slowing down of subjective time, it seems that the consumption of foods that charge/accelerate the “internal clock” is desirable for this purpose.
PB  - Elsevier Ltd.
T2  - Medical Hypotheses
T1  - Can food affect the perception of time?
VL  - 184
DO  - 10.1016/j.mehy.2024.111283
SP  - 111283
ER  - 

@article{
author = "Ajdžanović, Vladimir and Miler, Marko and Šošić-Jurjević, Branka and Filipović, Branko",
year = "2024",
abstract = "Time perception is an important and to some extent adjustable aspect of the human cognitive sphere. We hypothesize that the specific food consumption can affect the perception of time in two ways, namely through the emotions it triggers and through the nutrients it contains, which may affect brain chemistry. The “internal clock”, responsible for timing tasks, can be charged (accelerated) by arousal caused by visual food stimuli or uncharged (slowed down) in the same context by distraction of attention, leading to overestimation or underestimation of time, respectively. Nutrients can affect the “internal clock” rate in terms of increasing or decreasing it, which affects time estimation in the same way. Animal studies indicate that a protein meal, rich in the amino acid tyrosine, can increase the release of dopamine in the brain and speed up the “internal clock”. Furthermore, a carbohydrate meal can change the balance of amino acids in the brain in favor of tryptophan, which in turn can increase the release of serotonin and slow down the “internal clock”. Given that the modern, fast-paced lifestyle requires the slowing down of subjective time, it seems that the consumption of foods that charge/accelerate the “internal clock” is desirable for this purpose.",
publisher = "Elsevier Ltd.",
journal = "Medical Hypotheses",
title = "Can food affect the perception of time?",
volume = "184",
doi = "10.1016/j.mehy.2024.111283",
pages = "111283"
}

Ajdžanović, V., Miler, M., Šošić-Jurjević, B.,& Filipović, B.. (2024). Can food affect the perception of time?. in Medical Hypotheses
Elsevier Ltd.., 184, 111283.
https://doi.org/10.1016/j.mehy.2024.111283

Ajdžanović V, Miler M, Šošić-Jurjević B, Filipović B. Can food affect the perception of time?. in Medical Hypotheses. 2024;184:111283.
doi:10.1016/j.mehy.2024.111283 .

Ajdžanović, Vladimir, Miler, Marko, Šošić-Jurjević, Branka, Filipović, Branko, "Can food affect the perception of time?" in Medical Hypotheses, 184 (2024):111283,
https://doi.org/10.1016/j.mehy.2024.111283 . .

TY  - JOUR
AU  - Šošić-Jurjević, Branka
AU  - Lütjohann, Dieter
AU  - Trifunović, Svetlana
AU  - Pavlović, Slađan
AU  - Borković-Mitić, Slavica
AU  - Jovanović, Ljubiša
AU  - Ristić, Nataša
AU  - Ljiljana, Marina
AU  - Ajdžanović, Vladimir
AU  - Filipović, Branko
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6120
AB  - Age and sex influence serum cholesterol levels, but the underlying mechanisms remain
unclear. To investigate further, we measured cholesterol, precursors (surrogate synthesis markers),
degradation products (oxysterols and bile acid precursors) in serum, the liver, jejunum, and ileum,
as well as serum plant sterols (intestinal absorption markers) in male and female Wistar rats (4 and
24 months old). The analysis of histomorphometric and oxidative stress parameters (superoxide
dismutase, catalase, glutathione-related enzyme activities, lipid peroxide, and protein carbonyl concentrations) in the liver and jejunum offered further insights into the age- and sex-related differences.
The hepatic gene expression analysis included AR, ERα, and sex-specific growth hormone-regulated
(Cyp2c11 and Cyp2c12) and thyroid-responsive (Dio1, Tbg, and Spot 14) genes by qPCR. We observed
age-related changes in both sexes, with greater prominence in females. Aged females had significantly
higher serum cholesterol (p < 0.05), jejunum cholesterol (p < 0.05), and serum plant sterols (p < 0.05).
They exhibited poorer hepato-intestinal health compared with males, which was characterized by
mild liver dysfunction (hydropic degeneration, increased serum ALT, p < 0.05, and decreased activity
of some antioxidant defense enzymes, p < 0.05), mononuclear inflammation in the jejunal lamina
propria, and age-related decreases in jejunal catalase and glutathione peroxidase activity (p < 0.05).
Aged females showed increased levels of 27-hydroxycholesterol (p < 0.05) and upregulated ERα gene
expression (p < 0.05) in the liver. Our study suggests that the more significant age-related increase in
serum cholesterol in females is associated with poorer hepato-intestinal health and increased jejunal
cholesterol absorption. The local increase in 27-hydroxycholesterol during aging might reduce the
hepatoprotective effects of endogenous estrogen in the female liver.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Differences in Cholesterol Metabolism, Hepato-Intestinal Aging, and Hepatic Endocrine Milieu in Rats as Affected by the Sex and Age
IS  - 16
VL  - 24
DO  - 10.3390/ijms241612624
SP  - 12624
ER  - 

@article{
author = "Šošić-Jurjević, Branka and Lütjohann, Dieter and Trifunović, Svetlana and Pavlović, Slađan and Borković-Mitić, Slavica and Jovanović, Ljubiša and Ristić, Nataša and Ljiljana, Marina and Ajdžanović, Vladimir and Filipović, Branko",
year = "2023",
abstract = "Age and sex influence serum cholesterol levels, but the underlying mechanisms remain
unclear. To investigate further, we measured cholesterol, precursors (surrogate synthesis markers),
degradation products (oxysterols and bile acid precursors) in serum, the liver, jejunum, and ileum,
as well as serum plant sterols (intestinal absorption markers) in male and female Wistar rats (4 and
24 months old). The analysis of histomorphometric and oxidative stress parameters (superoxide
dismutase, catalase, glutathione-related enzyme activities, lipid peroxide, and protein carbonyl concentrations) in the liver and jejunum offered further insights into the age- and sex-related differences.
The hepatic gene expression analysis included AR, ERα, and sex-specific growth hormone-regulated
(Cyp2c11 and Cyp2c12) and thyroid-responsive (Dio1, Tbg, and Spot 14) genes by qPCR. We observed
age-related changes in both sexes, with greater prominence in females. Aged females had significantly
higher serum cholesterol (p < 0.05), jejunum cholesterol (p < 0.05), and serum plant sterols (p < 0.05).
They exhibited poorer hepato-intestinal health compared with males, which was characterized by
mild liver dysfunction (hydropic degeneration, increased serum ALT, p < 0.05, and decreased activity
of some antioxidant defense enzymes, p < 0.05), mononuclear inflammation in the jejunal lamina
propria, and age-related decreases in jejunal catalase and glutathione peroxidase activity (p < 0.05).
Aged females showed increased levels of 27-hydroxycholesterol (p < 0.05) and upregulated ERα gene
expression (p < 0.05) in the liver. Our study suggests that the more significant age-related increase in
serum cholesterol in females is associated with poorer hepato-intestinal health and increased jejunal
cholesterol absorption. The local increase in 27-hydroxycholesterol during aging might reduce the
hepatoprotective effects of endogenous estrogen in the female liver.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Differences in Cholesterol Metabolism, Hepato-Intestinal Aging, and Hepatic Endocrine Milieu in Rats as Affected by the Sex and Age",
number = "16",
volume = "24",
doi = "10.3390/ijms241612624",
pages = "12624"
}

Šošić-Jurjević, B., Lütjohann, D., Trifunović, S., Pavlović, S., Borković-Mitić, S., Jovanović, L., Ristić, N., Ljiljana, M., Ajdžanović, V.,& Filipović, B.. (2023). Differences in Cholesterol Metabolism, Hepato-Intestinal Aging, and Hepatic Endocrine Milieu in Rats as Affected by the Sex and Age. in International Journal of Molecular Sciences
Basel: MDPI., 24(16), 12624.
https://doi.org/10.3390/ijms241612624

Šošić-Jurjević B, Lütjohann D, Trifunović S, Pavlović S, Borković-Mitić S, Jovanović L, Ristić N, Ljiljana M, Ajdžanović V, Filipović B. Differences in Cholesterol Metabolism, Hepato-Intestinal Aging, and Hepatic Endocrine Milieu in Rats as Affected by the Sex and Age. in International Journal of Molecular Sciences. 2023;24(16):12624.
doi:10.3390/ijms241612624 .

Šošić-Jurjević, Branka, Lütjohann, Dieter, Trifunović, Svetlana, Pavlović, Slađan, Borković-Mitić, Slavica, Jovanović, Ljubiša, Ristić, Nataša, Ljiljana, Marina, Ajdžanović, Vladimir, Filipović, Branko, "Differences in Cholesterol Metabolism, Hepato-Intestinal Aging, and Hepatic Endocrine Milieu in Rats as Affected by the Sex and Age" in International Journal of Molecular Sciences, 24, no. 16 (2023):12624,
https://doi.org/10.3390/ijms241612624 . .

TY  - CONF
AU  - Ajdžanović, Vladimir
AU  - Čukuranović-Kokoris, Jovana
AU  - Miler, Marko
AU  - Šošić-Jurjević, Branka
AU  - Živanović, Jasmina
AU  - Marković Filipović, Jelena
AU  - Milošević, Verica
AU  - Filipović, Branko
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6405
AB  - Uvod. Aplikacija daidzeina može predstavljati efikasnu i manje rizičnu altemativu indikovanoj, klasičnoj estrogenizaciji starijih muškaraca. Cilj ove studije je podrazumevao morfofiziološku analizu kore nadbubrežne žlezde u pacovskom modelu uznapredovale andropauze, nakon primene daidzeina.
Materijal i metode. Sredovečni (16 meseci stari) pacovi Wistar soja su razdeljeni u
lažno operisanu (SO; n=8), orhidektomisanu (Orx; n=8) i daidzeinom tretiranu orhidektomisanu (Orx+D; n=8) grupu. Daidzein (30 mg/kg t.m./dan) je subkutano davan tri nedelje, dok su SO i Orx grupa dobijale samo rastvarač tokom istog perioda. Ciljevi su realizovani korišćenjem stereologije, histohemije/imunohistohemije, imunoeseja i ultrastruktume analize.
Rezultati. Tretman daidzeinom je značajno uvećao volumene ćelija i jedara zone glomeruloze 
za 32% i 41% tim redom, ali je i snizio (p<0.05) nivo cirkulišućeg aldosterona za 61%, sve u 
poređenju sa odgovarajućim parametrima kod Orx zivotinja. Takođe, daidzein je značajno smanjio 
volumen jedara ćelija zone fascikulate, kao i tkivni i cirkulišući nivo kortikosterona, za 24%, 48% i 33% 
navedenim redom, te izrazito (p<0.05) povisio cirkulišući nivo DHEA za oko 4.6 puta, sve u 
poređenju sa odgovarajućim parametrima kod Orx pacova. Daidzeinom prouzrokovano preusmeravanje 
puteva kortikosteroidogeneze ka produkciji adrenalnih androgena bilo je u skladu sa ultrastruktumim nalazom i rearanžmanima kapilarne rnreže u okviru konkretnih zona kore nadbubrežne zlezde.
Zaključak. Imajući u vidu sklad efekata daidzeina u ispitivanom kontekstu, kao relativnu bezbednost njegove upotrebe, moglo bi se reći da daidzein ima perspektivu u tretrnanu starenjem uzorokovanog gubitka androgena, te hiperfunkcije hipotalamo-hipofizno-adrenalne ose i povezanih metaboličkih poremećaja kod muškaraca.
AB  - Introduction. Daidzein application may represent effective and less harmful alternative to indicated, classical estrogenization of the aging men. The aim of this study was to perform morphophysiological analysis of the adrenal cortex, after daidzein supplementation in a rat model of the severe andropause.
Material and methods. Middle-aged (16 months old) Wistar rats were divided into sham operated (SO; n=8), orchidectomized (Orx; n=8) and daidzein treated orchidectomized (Orx+D; n=8) groups. Daidzein (30 mg/kg b.m./day) was administered subcutaneously for three weeks, while the SO and Orx groups received the vehicle alone. Set objectives were achieved using stereology, histochemistry/immunohistochemistry, immunoassays and ultrastructural analysis.
Results. Daidzein treatment significantly increased volumes of the zona glomerulosa cell and nuclei by 32% and 41% respectively, but decreased (p<0.05) circulating aldosterone levels by 61%, all in comparison with the adequate parameters in Orx animals. Also, daidzein significantly decreased zona fasciculata cell nuclei volume, as well as both the adrenal and circulating levels of corticosterone, by 24%, 48% and 33% respectively, but strongly (p<0.05) increased the circulating level of DHEA by about 4.6 times, all when compared to the corresponding parameters of Orx rats. Daidzein-induced shunting of the corticosteroidogenesis pathways towards adrenal androgens production corresponded to the ultrastructural findings and zonal capillary network rearrangements.
Conclusion. Given the coherence of its effects and relative safety, daidzein could be the remedy of choice for the treatment of aging-caused androgen deprivation and the hypothalamo-pituitary-adrenal axis hyperfunction/related metabolic issues in males.
PB  - Niš: University of Niš, Faculty of Medicine
C3  - Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia
T1  - Morfofiziološke karakteristike kore nadbubrežne žlezde nakon aplikacije daidzeina u pacovskom modelu uznapredovale andropauze
T1  - Morphophysiology of the adrenal cortex after daidzein application in a rat model of the severe andropause
SP  - 166
EP  - 167
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6405
ER  - 

@conference{
author = "Ajdžanović, Vladimir and Čukuranović-Kokoris, Jovana and Miler, Marko and Šošić-Jurjević, Branka and Živanović, Jasmina and Marković Filipović, Jelena and Milošević, Verica and Filipović, Branko",
year = "2023",
abstract = "Uvod. Aplikacija daidzeina može predstavljati efikasnu i manje rizičnu altemativu indikovanoj, klasičnoj estrogenizaciji starijih muškaraca. Cilj ove studije je podrazumevao morfofiziološku analizu kore nadbubrežne žlezde u pacovskom modelu uznapredovale andropauze, nakon primene daidzeina.
Materijal i metode. Sredovečni (16 meseci stari) pacovi Wistar soja su razdeljeni u
lažno operisanu (SO; n=8), orhidektomisanu (Orx; n=8) i daidzeinom tretiranu orhidektomisanu (Orx+D; n=8) grupu. Daidzein (30 mg/kg t.m./dan) je subkutano davan tri nedelje, dok su SO i Orx grupa dobijale samo rastvarač tokom istog perioda. Ciljevi su realizovani korišćenjem stereologije, histohemije/imunohistohemije, imunoeseja i ultrastruktume analize.
Rezultati. Tretman daidzeinom je značajno uvećao volumene ćelija i jedara zone glomeruloze 
za 32% i 41% tim redom, ali je i snizio (p<0.05) nivo cirkulišućeg aldosterona za 61%, sve u 
poređenju sa odgovarajućim parametrima kod Orx zivotinja. Takođe, daidzein je značajno smanjio 
volumen jedara ćelija zone fascikulate, kao i tkivni i cirkulišući nivo kortikosterona, za 24%, 48% i 33% 
navedenim redom, te izrazito (p<0.05) povisio cirkulišući nivo DHEA za oko 4.6 puta, sve u 
poređenju sa odgovarajućim parametrima kod Orx pacova. Daidzeinom prouzrokovano preusmeravanje 
puteva kortikosteroidogeneze ka produkciji adrenalnih androgena bilo je u skladu sa ultrastruktumim nalazom i rearanžmanima kapilarne rnreže u okviru konkretnih zona kore nadbubrežne zlezde.
Zaključak. Imajući u vidu sklad efekata daidzeina u ispitivanom kontekstu, kao relativnu bezbednost njegove upotrebe, moglo bi se reći da daidzein ima perspektivu u tretrnanu starenjem uzorokovanog gubitka androgena, te hiperfunkcije hipotalamo-hipofizno-adrenalne ose i povezanih metaboličkih poremećaja kod muškaraca., Introduction. Daidzein application may represent effective and less harmful alternative to indicated, classical estrogenization of the aging men. The aim of this study was to perform morphophysiological analysis of the adrenal cortex, after daidzein supplementation in a rat model of the severe andropause.
Material and methods. Middle-aged (16 months old) Wistar rats were divided into sham operated (SO; n=8), orchidectomized (Orx; n=8) and daidzein treated orchidectomized (Orx+D; n=8) groups. Daidzein (30 mg/kg b.m./day) was administered subcutaneously for three weeks, while the SO and Orx groups received the vehicle alone. Set objectives were achieved using stereology, histochemistry/immunohistochemistry, immunoassays and ultrastructural analysis.
Results. Daidzein treatment significantly increased volumes of the zona glomerulosa cell and nuclei by 32% and 41% respectively, but decreased (p<0.05) circulating aldosterone levels by 61%, all in comparison with the adequate parameters in Orx animals. Also, daidzein significantly decreased zona fasciculata cell nuclei volume, as well as both the adrenal and circulating levels of corticosterone, by 24%, 48% and 33% respectively, but strongly (p<0.05) increased the circulating level of DHEA by about 4.6 times, all when compared to the corresponding parameters of Orx rats. Daidzein-induced shunting of the corticosteroidogenesis pathways towards adrenal androgens production corresponded to the ultrastructural findings and zonal capillary network rearrangements.
Conclusion. Given the coherence of its effects and relative safety, daidzein could be the remedy of choice for the treatment of aging-caused androgen deprivation and the hypothalamo-pituitary-adrenal axis hyperfunction/related metabolic issues in males.",
publisher = "Niš: University of Niš, Faculty of Medicine",
journal = "Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia",
title = "Morfofiziološke karakteristike kore nadbubrežne žlezde nakon aplikacije daidzeina u pacovskom modelu uznapredovale andropauze, Morphophysiology of the adrenal cortex after daidzein application in a rat model of the severe andropause",
pages = "166-167",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6405"
}

Ajdžanović, V., Čukuranović-Kokoris, J., Miler, M., Šošić-Jurjević, B., Živanović, J., Marković Filipović, J., Milošević, V.,& Filipović, B.. (2023). Morfofiziološke karakteristike kore nadbubrežne žlezde nakon aplikacije daidzeina u pacovskom modelu uznapredovale andropauze. in Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia
Niš: University of Niš, Faculty of Medicine., 166-167.
https://hdl.handle.net/21.15107/rcub_ibiss_6405

Ajdžanović V, Čukuranović-Kokoris J, Miler M, Šošić-Jurjević B, Živanović J, Marković Filipović J, Milošević V, Filipović B. Morfofiziološke karakteristike kore nadbubrežne žlezde nakon aplikacije daidzeina u pacovskom modelu uznapredovale andropauze. in Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia. 2023;:166-167.
https://hdl.handle.net/21.15107/rcub_ibiss_6405 .

Ajdžanović, Vladimir, Čukuranović-Kokoris, Jovana, Miler, Marko, Šošić-Jurjević, Branka, Živanović, Jasmina, Marković Filipović, Jelena, Milošević, Verica, Filipović, Branko, "Morfofiziološke karakteristike kore nadbubrežne žlezde nakon aplikacije daidzeina u pacovskom modelu uznapredovale andropauze" in Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia (2023):166-167,
https://hdl.handle.net/21.15107/rcub_ibiss_6405 .

TY  - JOUR
AU  - Ajdžanović, Vladimir
AU  - Miler, Marko
AU  - Šošić-Jurjević, Branka
AU  - Filipović, Branko
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6279
AB  - Prostate health is related to the frequency of ejaculation and prostate drainage. Frequent, repetitive ejaculations theoretically may pose a prostate cancer risk due to ensuing high testosterone levels and loss of zinc. In line with this, the proximity of the human rectum and prostate is of diagnostic and therapeutic importance. We provide evidence to support the view that the proximity of the rectum and prostate in men enables lower bowel distention to compress the prostate's lateral side to facilitate drainage, which might lower cancer risk. This drainage mechanism is likely on-going and gradual, compared to rapid drainage following ejaculation. The physiological context suggests that these mechanisms of prostate drainage are complementary. Dietary content containing polyphenol- and fiber-rich meals, along with voluminous stool formation and increased gastrointestinal transit may all help improve prostate drainage. This may be important in voluntary abstinent men or other categories of sexually limited men. Recognizing that the anatomic relationship between the rectal area and prostate may facilitate prostate drainage, because of diet and gastro-intestinal activity, could be important to prostate health maintenance.
PB  - MRE Press
T2  - Journal of Men's Health
T1  - The close proximity between the human rectum and prostate: a functional-anatomic arrangement favoring prostate health
IS  - 10
VL  - 19
DO  - 10.22514/jomh.2023.094
SP  - 1
EP  - 6
ER  - 

@article{
author = "Ajdžanović, Vladimir and Miler, Marko and Šošić-Jurjević, Branka and Filipović, Branko",
year = "2023",
abstract = "Prostate health is related to the frequency of ejaculation and prostate drainage. Frequent, repetitive ejaculations theoretically may pose a prostate cancer risk due to ensuing high testosterone levels and loss of zinc. In line with this, the proximity of the human rectum and prostate is of diagnostic and therapeutic importance. We provide evidence to support the view that the proximity of the rectum and prostate in men enables lower bowel distention to compress the prostate's lateral side to facilitate drainage, which might lower cancer risk. This drainage mechanism is likely on-going and gradual, compared to rapid drainage following ejaculation. The physiological context suggests that these mechanisms of prostate drainage are complementary. Dietary content containing polyphenol- and fiber-rich meals, along with voluminous stool formation and increased gastrointestinal transit may all help improve prostate drainage. This may be important in voluntary abstinent men or other categories of sexually limited men. Recognizing that the anatomic relationship between the rectal area and prostate may facilitate prostate drainage, because of diet and gastro-intestinal activity, could be important to prostate health maintenance.",
publisher = "MRE Press",
journal = "Journal of Men's Health",
title = "The close proximity between the human rectum and prostate: a functional-anatomic arrangement favoring prostate health",
number = "10",
volume = "19",
doi = "10.22514/jomh.2023.094",
pages = "1-6"
}

Ajdžanović, V., Miler, M., Šošić-Jurjević, B.,& Filipović, B.. (2023). The close proximity between the human rectum and prostate: a functional-anatomic arrangement favoring prostate health. in Journal of Men's Health
MRE Press., 19(10), 1-6.
https://doi.org/10.22514/jomh.2023.094

Ajdžanović V, Miler M, Šošić-Jurjević B, Filipović B. The close proximity between the human rectum and prostate: a functional-anatomic arrangement favoring prostate health. in Journal of Men's Health. 2023;19(10):1-6.
doi:10.22514/jomh.2023.094 .

Ajdžanović, Vladimir, Miler, Marko, Šošić-Jurjević, Branka, Filipović, Branko, "The close proximity between the human rectum and prostate: a functional-anatomic arrangement favoring prostate health" in Journal of Men's Health, 19, no. 10 (2023):1-6,
https://doi.org/10.22514/jomh.2023.094 . .

TY  - JOUR
AU  - Trifunović, Svetlana
AU  - Šošić-Jurjević, Branka 
AU  - Ristić, Nataša
AU  - Nestorović, Nataša
AU  - Filipović, Branko
AU  - Stevanović, Ivana
AU  - Begović-Kuprešanin, Vesna
AU  - Manojlović-Stojanoski, Milica
PY  - 2023
UR  - https://www.mdpi.com/1422-0067/24/1/540
UR  - http://www.ncbi.nlm.nih.gov/pubmed/36613982
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9820254
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5402
AB  - As the mediator between the mother and fetus, the placenta allows the most appropriate environment and optimal fetal growth. The placenta of one sex sometimes has a greater ability over the other to respond to and protect against possible maternal insults. Here, we characterized sex differences in the placenta's morphological features and antioxidant status following dexamethasone (Dx) exposure. Pregnant rats were exposed to Dx or saline. The placenta was histologically and stereologically analyzed. The activity of the antioxidant enzymes, lipid peroxides (TBARS), superoxide anion and nitric oxide (NO) was measured. The decrease in placental zone volumes was more pronounced (p < 0.05) in female placentas. The volume density of PCNA-immunopositive nuclei was reduced (p < 0.05) in both sexes. The reduced (p < 0.05) antioxidant enzyme activities, enhanced TBARS and NO concentration indicate that Dx exposure triggered oxidative stress in the placenta of both fetal sexes, albeit stronger in the placenta of female fetuses. In conclusion, maternal Dx treatment reduced the size and volume of placental zones, altered placental histomorphology, decreased cell proliferation and triggered oxidative stress; however, the placentas of female fetuses exerted more significant responses to the treatment effects. The reduced placental size most probably reduced the transport of nutrients and oxygen, thus resulting in the reduced weight of fetuses, similar in both sexes. The lesser ability of the male placenta to detect and react to maternal exposure to environmental challenges may lead to long-standing health effects.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Maternal Dexamethasone Exposure Induces Sex-Specific Changes in Histomorphology and Redox Homeostasis of Rat Placenta
IS  - 1
VL  - 24
DO  - 10.3390/ijms24010540
SP  - 540
ER  - 

@article{
author = "Trifunović, Svetlana and Šošić-Jurjević, Branka  and Ristić, Nataša and Nestorović, Nataša and Filipović, Branko and Stevanović, Ivana and Begović-Kuprešanin, Vesna and Manojlović-Stojanoski, Milica",
year = "2023",
abstract = "As the mediator between the mother and fetus, the placenta allows the most appropriate environment and optimal fetal growth. The placenta of one sex sometimes has a greater ability over the other to respond to and protect against possible maternal insults. Here, we characterized sex differences in the placenta's morphological features and antioxidant status following dexamethasone (Dx) exposure. Pregnant rats were exposed to Dx or saline. The placenta was histologically and stereologically analyzed. The activity of the antioxidant enzymes, lipid peroxides (TBARS), superoxide anion and nitric oxide (NO) was measured. The decrease in placental zone volumes was more pronounced (p < 0.05) in female placentas. The volume density of PCNA-immunopositive nuclei was reduced (p < 0.05) in both sexes. The reduced (p < 0.05) antioxidant enzyme activities, enhanced TBARS and NO concentration indicate that Dx exposure triggered oxidative stress in the placenta of both fetal sexes, albeit stronger in the placenta of female fetuses. In conclusion, maternal Dx treatment reduced the size and volume of placental zones, altered placental histomorphology, decreased cell proliferation and triggered oxidative stress; however, the placentas of female fetuses exerted more significant responses to the treatment effects. The reduced placental size most probably reduced the transport of nutrients and oxygen, thus resulting in the reduced weight of fetuses, similar in both sexes. The lesser ability of the male placenta to detect and react to maternal exposure to environmental challenges may lead to long-standing health effects.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Maternal Dexamethasone Exposure Induces Sex-Specific Changes in Histomorphology and Redox Homeostasis of Rat Placenta",
number = "1",
volume = "24",
doi = "10.3390/ijms24010540",
pages = "540"
}

Trifunović, S., Šošić-Jurjević, B., Ristić, N., Nestorović, N., Filipović, B., Stevanović, I., Begović-Kuprešanin, V.,& Manojlović-Stojanoski, M.. (2023). Maternal Dexamethasone Exposure Induces Sex-Specific Changes in Histomorphology and Redox Homeostasis of Rat Placenta. in International Journal of Molecular Sciences
Basel: MDPI., 24(1), 540.
https://doi.org/10.3390/ijms24010540

Trifunović S, Šošić-Jurjević B, Ristić N, Nestorović N, Filipović B, Stevanović I, Begović-Kuprešanin V, Manojlović-Stojanoski M. Maternal Dexamethasone Exposure Induces Sex-Specific Changes in Histomorphology and Redox Homeostasis of Rat Placenta. in International Journal of Molecular Sciences. 2023;24(1):540.
doi:10.3390/ijms24010540 .

Trifunović, Svetlana, Šošić-Jurjević, Branka , Ristić, Nataša, Nestorović, Nataša, Filipović, Branko, Stevanović, Ivana, Begović-Kuprešanin, Vesna, Manojlović-Stojanoski, Milica, "Maternal Dexamethasone Exposure Induces Sex-Specific Changes in Histomorphology and Redox Homeostasis of Rat Placenta" in International Journal of Molecular Sciences, 24, no. 1 (2023):540,
https://doi.org/10.3390/ijms24010540 . .

TY  - JOUR
AU  - Ajdžanović, Vladimir
AU  - Šošić-Jurjević, Branka
AU  - Ranin, Jovan
AU  - Filipović, Branko
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6078
AB  - In his Theory of relativity, Einstein determined that the time is relative to the reference frame of the observer. Under specific conditions, there is a difference in the elapsed time between two clocks, known as time dilation. A similar relativistic effect could be attributed to the brain operating at different frequencies, e.g. while it is slow and during the thought process. Time flow and the aging process are causally linked. Herein, we introduce physical relativity into the mind/thought context and elaborate changed perception of the time flow (the impression of the time acceleration) with aging. The phenomenology of time is observed in the context of physical and biological clock, as well as by introducing the category of ‘mind time’. Mental processing impairment is crucial for the “aging-caused relativity of time”, while adjusting of its’ perception seems to be a matter of body/mind rest, mental hygiene and physical activity of the aging subject. We also provide a brief overview of the perception of time flow in some disease states that coincide with aging. Our main idea has a perspective for future development in the interdisciplinary synergy of philosophy, physical-mathematical elaboration, experimental biology and clinical investigations.
PB  - Heidelberg: Springer Nature
T2  - Biologia Futura
T1  - Biologia Futura: does the aging process contribute to the relativity of time?
IS  - 74
IS  - 1-2
DO  - 10.1007/s42977-023-00167-2
SP  - 137
EP  - 143
ER  - 

@article{
author = "Ajdžanović, Vladimir and Šošić-Jurjević, Branka and Ranin, Jovan and Filipović, Branko",
year = "2023",
abstract = "In his Theory of relativity, Einstein determined that the time is relative to the reference frame of the observer. Under specific conditions, there is a difference in the elapsed time between two clocks, known as time dilation. A similar relativistic effect could be attributed to the brain operating at different frequencies, e.g. while it is slow and during the thought process. Time flow and the aging process are causally linked. Herein, we introduce physical relativity into the mind/thought context and elaborate changed perception of the time flow (the impression of the time acceleration) with aging. The phenomenology of time is observed in the context of physical and biological clock, as well as by introducing the category of ‘mind time’. Mental processing impairment is crucial for the “aging-caused relativity of time”, while adjusting of its’ perception seems to be a matter of body/mind rest, mental hygiene and physical activity of the aging subject. We also provide a brief overview of the perception of time flow in some disease states that coincide with aging. Our main idea has a perspective for future development in the interdisciplinary synergy of philosophy, physical-mathematical elaboration, experimental biology and clinical investigations.",
publisher = "Heidelberg: Springer Nature",
journal = "Biologia Futura",
title = "Biologia Futura: does the aging process contribute to the relativity of time?",
number = "74, 1-2",
doi = "10.1007/s42977-023-00167-2",
pages = "137-143"
}

Ajdžanović, V., Šošić-Jurjević, B., Ranin, J.,& Filipović, B.. (2023). Biologia Futura: does the aging process contribute to the relativity of time?. in Biologia Futura
Heidelberg: Springer Nature.(74), 137-143.
https://doi.org/10.1007/s42977-023-00167-2

Ajdžanović V, Šošić-Jurjević B, Ranin J, Filipović B. Biologia Futura: does the aging process contribute to the relativity of time?. in Biologia Futura. 2023;(74):137-143.
doi:10.1007/s42977-023-00167-2 .

Ajdžanović, Vladimir, Šošić-Jurjević, Branka, Ranin, Jovan, Filipović, Branko, "Biologia Futura: does the aging process contribute to the relativity of time?" in Biologia Futura, no. 74 (2023):137-143,
https://doi.org/10.1007/s42977-023-00167-2 . .

TY  - CONF
AU  - Ćirić, Jelena
AU  - Milovanović, Nikola
AU  - Jovanović Macura, Irena
AU  - Tešić, Vesna
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka 
AU  - Perović, Milka
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5847
AB  - Targeting novel pathways contributing to the pathogenesis/progression of Alzheimer’s disease (AD) is crucial due to the lack of effective management and treatment modalities. Higher prevalence, progression rate and severity of AD in women than in men also establish sex as key variable in AD therapy development.Thyroid disorders, both hyper- and hypothyroidism, were found to occur with up to nine-fold higher prevalence in women compared to men. The molecular mechanisms by which thyroid dysfunction contribute to AD pathogenesis and heterogeneity remain however elusive. We therefore examined sex-related alterations in gene expression of iodothyronine deiodinase 2 (Dio2) and transthyretin (TTR) involved in the tissue metabolism and the distribution of thyroid hormones (THs), respectively, in a novel, state-of the art knock-in (KI) mouse model of AD-like amyloidosis, APPNL-G-F mice.Quantitative RT-PCR analysis revealed prominent differences in cortical Dio2 and TTR gene expression in 9-month-old male and female APPNL-G-F mice and their non KI littermates (WT). In comparison to WT male mice, the increase in Dio2 mRNA level was evident in female WT mice, while a trend toward a decrease was detected in their APPNL-G-F KI littermates. Expression in the opposite direction was observed for TTR, with a robust genotype-dependent decrease in male mice.Results are in line with well-established role of THs in the regulation of neuronal plasticity in the adult brain and suggest profound sex-biased effects of TH on Aβ induced pathology in APPNL-G-F mice.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković" – National Institute of Republic of Serbia, University of Belgrade
C3  - 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
T1  - Thyroid hormone metabolism in the cortex of male and female APP knock-in mice
SP  - 111
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5847
ER  - 

@conference{
author = "Ćirić, Jelena and Milovanović, Nikola and Jovanović Macura, Irena and Tešić, Vesna and Filipović, Branko and Šošić-Jurjević, Branka  and Perović, Milka",
year = "2023",
abstract = "Targeting novel pathways contributing to the pathogenesis/progression of Alzheimer’s disease (AD) is crucial due to the lack of effective management and treatment modalities. Higher prevalence, progression rate and severity of AD in women than in men also establish sex as key variable in AD therapy development.Thyroid disorders, both hyper- and hypothyroidism, were found to occur with up to nine-fold higher prevalence in women compared to men. The molecular mechanisms by which thyroid dysfunction contribute to AD pathogenesis and heterogeneity remain however elusive. We therefore examined sex-related alterations in gene expression of iodothyronine deiodinase 2 (Dio2) and transthyretin (TTR) involved in the tissue metabolism and the distribution of thyroid hormones (THs), respectively, in a novel, state-of the art knock-in (KI) mouse model of AD-like amyloidosis, APPNL-G-F mice.Quantitative RT-PCR analysis revealed prominent differences in cortical Dio2 and TTR gene expression in 9-month-old male and female APPNL-G-F mice and their non KI littermates (WT). In comparison to WT male mice, the increase in Dio2 mRNA level was evident in female WT mice, while a trend toward a decrease was detected in their APPNL-G-F KI littermates. Expression in the opposite direction was observed for TTR, with a robust genotype-dependent decrease in male mice.Results are in line with well-established role of THs in the regulation of neuronal plasticity in the adult brain and suggest profound sex-biased effects of TH on Aβ induced pathology in APPNL-G-F mice.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković" – National Institute of Republic of Serbia, University of Belgrade",
journal = "8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia",
title = "Thyroid hormone metabolism in the cortex of male and female APP knock-in mice",
pages = "111",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5847"
}

Ćirić, J., Milovanović, N., Jovanović Macura, I., Tešić, V., Filipović, B., Šošić-Jurjević, B.,& Perović, M.. (2023). Thyroid hormone metabolism in the cortex of male and female APP knock-in mice. in 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković" – National Institute of Republic of Serbia, University of Belgrade., 111.
https://hdl.handle.net/21.15107/rcub_ibiss_5847

Ćirić J, Milovanović N, Jovanović Macura I, Tešić V, Filipović B, Šošić-Jurjević B, Perović M. Thyroid hormone metabolism in the cortex of male and female APP knock-in mice. in 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. 2023;:111.
https://hdl.handle.net/21.15107/rcub_ibiss_5847 .

Ćirić, Jelena, Milovanović, Nikola, Jovanović Macura, Irena, Tešić, Vesna, Filipović, Branko, Šošić-Jurjević, Branka , Perović, Milka, "Thyroid hormone metabolism in the cortex of male and female APP knock-in mice" in 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia (2023):111,
https://hdl.handle.net/21.15107/rcub_ibiss_5847 .

TY  - CONF
AU  - Ćirić, Jelena
AU  - Milovanović, Nikola
AU  - Jovanović Macura, Irena
AU  - Tešić, Vesna
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka 
AU  - Perović, Milka
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5846
AB  - Targeting novel pathways contributing to the pathogenesis/progression of Alzheimer’s 
disease (AD) is crucial due to the lack of effective management and treatment 
modalities. Higher prevalence, progression rate and severity of AD in women than in 
men also establish sex as key variable in AD therapy development.
Thyroid disorders, both hyper- and hypothyroidism, were found to occur with up to 
nine-fold higher prevalence in women compared to men. The molecular mechanisms 
by which thyroid dysfunction contribute to AD pathogenesis and heterogeneity remain 
however elusive. We therefore examined sex-related alterations in gene expression of 
iodothyronine deiodinase 2 (Dio2) and transthyretin (TTR) involved in the tissue 
metabolism and the distribution of thyroid hormones (THs), respectively, in a novel, 
state-of the art knock-in (KI) mouse model of AD-like amyloidosis, APPNL-G-F mice.
Quantitative RT-PCR analysis revealed prominent differences in cortical Dio2 and 
TTR gene expression in 9-month-old male and female APPNL-G-F mice and their non KI littermates (WT). In comparison to WT male mice, the increase in Dio2 mRNA 
level was evident in female WT mice, while a trend toward a decrease was detected in 
their APPNL-G-F KI littermates. Expression in the opposite direction was observed for 
TTR, with a robust genotype-dependent decrease in male mice.
Results are in line with well-established role of THs in the regulation of neuronal 
plasticity in the adult brain and suggest profound sex-biased effects of TH on Aβ induced pathology in APPNL-G-F mice.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
T1  - Thyroid hormone metabolism in the cortex of male and female APP knock-in mice
SP  - 111
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5846
ER  - 

@conference{
author = "Ćirić, Jelena and Milovanović, Nikola and Jovanović Macura, Irena and Tešić, Vesna and Filipović, Branko and Šošić-Jurjević, Branka  and Perović, Milka",
year = "2023",
abstract = "Targeting novel pathways contributing to the pathogenesis/progression of Alzheimer’s 
disease (AD) is crucial due to the lack of effective management and treatment 
modalities. Higher prevalence, progression rate and severity of AD in women than in 
men also establish sex as key variable in AD therapy development.
Thyroid disorders, both hyper- and hypothyroidism, were found to occur with up to 
nine-fold higher prevalence in women compared to men. The molecular mechanisms 
by which thyroid dysfunction contribute to AD pathogenesis and heterogeneity remain 
however elusive. We therefore examined sex-related alterations in gene expression of 
iodothyronine deiodinase 2 (Dio2) and transthyretin (TTR) involved in the tissue 
metabolism and the distribution of thyroid hormones (THs), respectively, in a novel, 
state-of the art knock-in (KI) mouse model of AD-like amyloidosis, APPNL-G-F mice.
Quantitative RT-PCR analysis revealed prominent differences in cortical Dio2 and 
TTR gene expression in 9-month-old male and female APPNL-G-F mice and their non KI littermates (WT). In comparison to WT male mice, the increase in Dio2 mRNA 
level was evident in female WT mice, while a trend toward a decrease was detected in 
their APPNL-G-F KI littermates. Expression in the opposite direction was observed for 
TTR, with a robust genotype-dependent decrease in male mice.
Results are in line with well-established role of THs in the regulation of neuronal 
plasticity in the adult brain and suggest profound sex-biased effects of TH on Aβ induced pathology in APPNL-G-F mice.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia",
title = "Thyroid hormone metabolism in the cortex of male and female APP knock-in mice",
pages = "111",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5846"
}

Ćirić, J., Milovanović, N., Jovanović Macura, I., Tešić, V., Filipović, B., Šošić-Jurjević, B.,& Perović, M.. (2023). Thyroid hormone metabolism in the cortex of male and female APP knock-in mice. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 111.
https://hdl.handle.net/21.15107/rcub_ibiss_5846

Ćirić J, Milovanović N, Jovanović Macura I, Tešić V, Filipović B, Šošić-Jurjević B, Perović M. Thyroid hormone metabolism in the cortex of male and female APP knock-in mice. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. 2023;:111.
https://hdl.handle.net/21.15107/rcub_ibiss_5846 .

Ćirić, Jelena, Milovanović, Nikola, Jovanović Macura, Irena, Tešić, Vesna, Filipović, Branko, Šošić-Jurjević, Branka , Perović, Milka, "Thyroid hormone metabolism in the cortex of male and female APP knock-in mice" in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia (2023):111,
https://hdl.handle.net/21.15107/rcub_ibiss_5846 .

TY  - CONF
AU  - Ristić, Nataša
AU  - Nestorović, Nataša
AU  - Trifunović, Svetlana
AU  - Šošić-Jurjević, Branka
AU  - Filipović, Branko
AU  - Ajdžanović, Vladimir
AU  - Živanović, Jasmina
AU  - Miler, Marko
AU  - Manojlović-Stojanoski, Milica
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6695
AB  - Развој организма и хомеостатски механизми се прилагођавају променљивим условима спољашње средине још током пренаталног периода. Женски репродуктивни систем активно реагује адаптирајући развој и диференцирање гонадотропних ћелија хипофизе и оваријума у складу са условима околине.1,2 Различити типови стреса, укључујући хормонске, нутритивне и психолошке изазове могу проузроковати бројне промене које обликују будући репродуктивни капацитет женке и заправо су део пренаталног програмирања физиологије организма. Третман гравидних женки дексаметазоном (Дк) значајно утиче на развој фетуса и опонаша антенаталну терапију гравидних жена глукокортикоидима као најчешће присутни третман у обстетричкој пракси. Стога су гравидне женке пацова Дк третиране (3 x 0,5 мг/кг/тм Дк) од 16. до 18. дана гестације. Пренатално Дк излагање изазвало је смањење масе фетуса непосредно пред рођење. Забележено је значајно смањење апсолутног броја ФСХ (фоликулостимулирајући хормон) и ЛХ (лутеинизирајући хормон) гонадотропних ћелија хипофизе (p < 0,05) од феталног, преко постнаталног, до пуберталног периода у односу на контролне вредности. Паралелно, Дк излагање током феталног развоја проузроковало је смањење волумена оваријума код потомака старих 16 и 38 дана (p < 0,05). Број фоликула је смањен код 16 дана старих потомака, док су поремећај процеса фоликулогенезе и одложен почетак пубертета забележени код потомака старих 38 дана. Можемо закључити да пренатални период развоја снажно утиче на фертилитет женки током репродуктивног периода.
AB  - Razvoj organizma i homeostatski mehanizmi se prilagođavaju promenljivim uslovima spoljašnje sredine još tokom prenatalnog perioda. Ženski reproduktivni sistem aktivno reaguje adaptirajući razvoj i diferenciranje gonadotropnih ćelija hipofize i ovarijuma u skladu sa uslovima okoline.1,2 Različiti tipovi stresa, uključujući hormonske, nutritivne i psihološke izazove mogu prouzrokovati brojne promene koje oblikuju budući reproduktivni kapacitet ženke i zapravo su deo prenatalnog programiranja fiziologije organizma. Tretman gravidnih ženki deksametazonom (Dk) značajno utiče na razvoj fetusa i oponaša antenatalnu terapiju gravidnih žena glukokortikoidima kao najčešće prisutni tretman u obstetričkoj praksi. Stoga su gravidne ženke pacova Dk tretirane (3 x 0,5 mg/kg/tm Dk) od 16. do 18. dana gestacije. Prenatalno Dk izlaganje izazvalo je smanjenje mase fetusa neposredno pred rođenje. Zabeleženo je značajno smanjenje apsolutnog broja FSH (folikulostimulirajući hormon) i LH (luteinizirajući hormon) gonadotropnih ćelija hipofize (p < 0,05) od fetalnog, preko postnatalnog, do pubertalnog perioda u odnosu na kontrolne vrednosti. Paralelno, Dk izlaganje tokom fetalnog razvoja prouzrokovalo je smanjenje volumena ovarijuma kod potomaka starih 16 i 38 dana (p < 0,05). Broj folikula je smanjen kod 16 dana starih potomaka, dok su poremećaj procesa folikulogeneze i odložen početak puberteta zabeleženi kod potomaka starih 38 dana. Možemo zaključiti da prenatalni period razvoja snažno utiče na fertilitet ženki tokom reproduktivnog perioda.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Развој женског репродуктивног система и последице пренаталног излагања дексаметазону
T1  - Razvoj ženskog reproduktivnog sistema i posledice prenatalnog izlaganja deksametazonu
SP  - 379
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6695
ER  - 

@conference{
author = "Ristić, Nataša and Nestorović, Nataša and Trifunović, Svetlana and Šošić-Jurjević, Branka and Filipović, Branko and Ajdžanović, Vladimir and Živanović, Jasmina and Miler, Marko and Manojlović-Stojanoski, Milica",
year = "2022",
abstract = "Развој организма и хомеостатски механизми се прилагођавају променљивим условима спољашње средине још током пренаталног периода. Женски репродуктивни систем активно реагује адаптирајући развој и диференцирање гонадотропних ћелија хипофизе и оваријума у складу са условима околине.1,2 Различити типови стреса, укључујући хормонске, нутритивне и психолошке изазове могу проузроковати бројне промене које обликују будући репродуктивни капацитет женке и заправо су део пренаталног програмирања физиологије организма. Третман гравидних женки дексаметазоном (Дк) значајно утиче на развој фетуса и опонаша антенаталну терапију гравидних жена глукокортикоидима као најчешће присутни третман у обстетричкој пракси. Стога су гравидне женке пацова Дк третиране (3 x 0,5 мг/кг/тм Дк) од 16. до 18. дана гестације. Пренатално Дк излагање изазвало је смањење масе фетуса непосредно пред рођење. Забележено је значајно смањење апсолутног броја ФСХ (фоликулостимулирајући хормон) и ЛХ (лутеинизирајући хормон) гонадотропних ћелија хипофизе (p < 0,05) од феталног, преко постнаталног, до пуберталног периода у односу на контролне вредности. Паралелно, Дк излагање током феталног развоја проузроковало је смањење волумена оваријума код потомака старих 16 и 38 дана (p < 0,05). Број фоликула је смањен код 16 дана старих потомака, док су поремећај процеса фоликулогенезе и одложен почетак пубертета забележени код потомака старих 38 дана. Можемо закључити да пренатални период развоја снажно утиче на фертилитет женки током репродуктивног периода., Razvoj organizma i homeostatski mehanizmi se prilagođavaju promenljivim uslovima spoljašnje sredine još tokom prenatalnog perioda. Ženski reproduktivni sistem aktivno reaguje adaptirajući razvoj i diferenciranje gonadotropnih ćelija hipofize i ovarijuma u skladu sa uslovima okoline.1,2 Različiti tipovi stresa, uključujući hormonske, nutritivne i psihološke izazove mogu prouzrokovati brojne promene koje oblikuju budući reproduktivni kapacitet ženke i zapravo su deo prenatalnog programiranja fiziologije organizma. Tretman gravidnih ženki deksametazonom (Dk) značajno utiče na razvoj fetusa i oponaša antenatalnu terapiju gravidnih žena glukokortikoidima kao najčešće prisutni tretman u obstetričkoj praksi. Stoga su gravidne ženke pacova Dk tretirane (3 x 0,5 mg/kg/tm Dk) od 16. do 18. dana gestacije. Prenatalno Dk izlaganje izazvalo je smanjenje mase fetusa neposredno pred rođenje. Zabeleženo je značajno smanjenje apsolutnog broja FSH (folikulostimulirajući hormon) i LH (luteinizirajući hormon) gonadotropnih ćelija hipofize (p < 0,05) od fetalnog, preko postnatalnog, do pubertalnog perioda u odnosu na kontrolne vrednosti. Paralelno, Dk izlaganje tokom fetalnog razvoja prouzrokovalo je smanjenje volumena ovarijuma kod potomaka starih 16 i 38 dana (p < 0,05). Broj folikula je smanjen kod 16 dana starih potomaka, dok su poremećaj procesa folikulogeneze i odložen početak puberteta zabeleženi kod potomaka starih 38 dana. Možemo zaključiti da prenatalni period razvoja snažno utiče na fertilitet ženki tokom reproduktivnog perioda.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Развој женског репродуктивног система и последице пренаталног излагања дексаметазону, Razvoj ženskog reproduktivnog sistema i posledice prenatalnog izlaganja deksametazonu",
pages = "379",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6695"
}

Ristić, N., Nestorović, N., Trifunović, S., Šošić-Jurjević, B., Filipović, B., Ajdžanović, V., Živanović, J., Miler, M.,& Manojlović-Stojanoski, M.. (2022). Развој женског репродуктивног система и последице пренаталног излагања дексаметазону. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 379.
https://hdl.handle.net/21.15107/rcub_ibiss_6695

Ristić N, Nestorović N, Trifunović S, Šošić-Jurjević B, Filipović B, Ajdžanović V, Živanović J, Miler M, Manojlović-Stojanoski M. Развој женског репродуктивног система и последице пренаталног излагања дексаметазону. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:379.
https://hdl.handle.net/21.15107/rcub_ibiss_6695 .

Ristić, Nataša, Nestorović, Nataša, Trifunović, Svetlana, Šošić-Jurjević, Branka, Filipović, Branko, Ajdžanović, Vladimir, Živanović, Jasmina, Miler, Marko, Manojlović-Stojanoski, Milica, "Развој женског репродуктивног система и последице пренаталног излагања дексаметазону" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):379,
https://hdl.handle.net/21.15107/rcub_ibiss_6695 .

TY  - JOUR
AU  - Ajduković, Maja
AU  - Vučić, Tijana
AU  - Cvijanović, Milena
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka 
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5032
AB  - Amphibians are useful bioindicators for monitoring aquatic health and the influence of xenobiotics such as endocrine disrupting chemicals. Because aquatic ecosystems experience the majority of global pollution, aquatic organisms are most exposed and vulnerable to endocrine disruptors. Furthermore, penetration of endocrine disruptors into aquatic organisms especially in amphibians is even easier because of more permeable skin, resulting in high bioavailability and bioaccumulation of chemicals. One of the most potent endocrine disruptors is thiourea, which chemically blocks the synthesis of thyroid hormones and prevents metamorphosis in amphibians. We investigated the influence of thiourea on histomorphology of the thyroid gland in Triturus newts at the metamorphic stage, when thyroid hormone concentrations should reach their maximum level. Chronic exposure to thiourea induced hypertrophy and hyperplasia of follicular cells as well as a significant reduction of interstitial tissue. The intensity of the thyroglobulin immunostaining signal significantly decreases upon chronic exposure to thiourea. Successful cross-reactivity of human primary antibody in immunochemical detection of thyroglobulin in Urodela confirms potential homology in thyroglobulin structure throughout the vertebrates.
PB  - Munich: Elsevier GmbH
T2  - Zoology
T1  - Changes in thyroid histomorphology and thyroglobulin immunostaining upon exposure to thiourea in Triturus newts
VL  - 155
DO  - 10.1016/j.zool.2022.126050
SP  - 126050
ER  - 

@article{
author = "Ajduković, Maja and Vučić, Tijana and Cvijanović, Milena and Filipović, Branko and Šošić-Jurjević, Branka ",
year = "2022",
abstract = "Amphibians are useful bioindicators for monitoring aquatic health and the influence of xenobiotics such as endocrine disrupting chemicals. Because aquatic ecosystems experience the majority of global pollution, aquatic organisms are most exposed and vulnerable to endocrine disruptors. Furthermore, penetration of endocrine disruptors into aquatic organisms especially in amphibians is even easier because of more permeable skin, resulting in high bioavailability and bioaccumulation of chemicals. One of the most potent endocrine disruptors is thiourea, which chemically blocks the synthesis of thyroid hormones and prevents metamorphosis in amphibians. We investigated the influence of thiourea on histomorphology of the thyroid gland in Triturus newts at the metamorphic stage, when thyroid hormone concentrations should reach their maximum level. Chronic exposure to thiourea induced hypertrophy and hyperplasia of follicular cells as well as a significant reduction of interstitial tissue. The intensity of the thyroglobulin immunostaining signal significantly decreases upon chronic exposure to thiourea. Successful cross-reactivity of human primary antibody in immunochemical detection of thyroglobulin in Urodela confirms potential homology in thyroglobulin structure throughout the vertebrates.",
publisher = "Munich: Elsevier GmbH",
journal = "Zoology",
title = "Changes in thyroid histomorphology and thyroglobulin immunostaining upon exposure to thiourea in Triturus newts",
volume = "155",
doi = "10.1016/j.zool.2022.126050",
pages = "126050"
}

Ajduković, M., Vučić, T., Cvijanović, M., Filipović, B.,& Šošić-Jurjević, B.. (2022). Changes in thyroid histomorphology and thyroglobulin immunostaining upon exposure to thiourea in Triturus newts. in Zoology
Munich: Elsevier GmbH., 155, 126050.
https://doi.org/10.1016/j.zool.2022.126050

Ajduković M, Vučić T, Cvijanović M, Filipović B, Šošić-Jurjević B. Changes in thyroid histomorphology and thyroglobulin immunostaining upon exposure to thiourea in Triturus newts. in Zoology. 2022;155:126050.
doi:10.1016/j.zool.2022.126050 .

Ajduković, Maja, Vučić, Tijana, Cvijanović, Milena, Filipović, Branko, Šošić-Jurjević, Branka , "Changes in thyroid histomorphology and thyroglobulin immunostaining upon exposure to thiourea in Triturus newts" in Zoology, 155 (2022):126050,
https://doi.org/10.1016/j.zool.2022.126050 . .

TY  - JOUR
AU  - Filipović, Branko
AU  - Ajdžanović, Vladimir
AU  - Živanović, Jasmina
AU  - Trifunović, Svetlana
AU  - Ristić, Nataša
AU  - Milošević, Verica
AU  - Šošić-Jurjević, Branka 
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4971
AB  - Thyroid C-cells secrete the hormone calcitonin (CT) which acts as an inhibitor of bone resorption. Our aim was to examine the age-related changes in the structure and function of CT-producing C-cells, using histomorphometric, ultrastructural, and biochemical analyses. We used young adult (3-months-old), middle-aged (16-months-old), and old (24-months-old) male rats. The peroxidase-antiperoxidase method was applied for localization of CT. Stereological analysis was performed using the newCAST stereological software package. Serum samples were analyzed for the determination of CT, testosterone (T), calcium (Ca2+), and phosphorus (P). We found a significant increase in the volume density (Vv) of C-cells in both older groups (p < 0.05). The percentage of smaller volume range C-cells increased (p < 0.0001), while the proportion of greater volume range C-cells decreased (p < 0.05) with ageing. Ultrastructural analysis revealed a larger number of secretory granules in older rats. Serum CT increased (p < 0.001), while serum T and P were reduced (p < 0.01) in older rats. Serum Ca2+ was lower (p < 0.0001) in middle-aged rats compared to young adults. We revealed a 20% incidence of C-cell hyperplasia in older rats and one case of medullary thyroid carcinoma in an old rat. Our findings indicate that the ageing process causes significant histomorphometric changes at the thyroid C-cell level.
PB  - New York: Cambridge University Press
T2  - Microscopy and Microanalysis
T1  - Age-Related Changes in Calcitonin-Producing Thyroid C-Cells of Male Wistar Rats
DO  - 10.1017/S1431927622000721
ER  - 

@article{
author = "Filipović, Branko and Ajdžanović, Vladimir and Živanović, Jasmina and Trifunović, Svetlana and Ristić, Nataša and Milošević, Verica and Šošić-Jurjević, Branka ",
year = "2022",
abstract = "Thyroid C-cells secrete the hormone calcitonin (CT) which acts as an inhibitor of bone resorption. Our aim was to examine the age-related changes in the structure and function of CT-producing C-cells, using histomorphometric, ultrastructural, and biochemical analyses. We used young adult (3-months-old), middle-aged (16-months-old), and old (24-months-old) male rats. The peroxidase-antiperoxidase method was applied for localization of CT. Stereological analysis was performed using the newCAST stereological software package. Serum samples were analyzed for the determination of CT, testosterone (T), calcium (Ca2+), and phosphorus (P). We found a significant increase in the volume density (Vv) of C-cells in both older groups (p < 0.05). The percentage of smaller volume range C-cells increased (p < 0.0001), while the proportion of greater volume range C-cells decreased (p < 0.05) with ageing. Ultrastructural analysis revealed a larger number of secretory granules in older rats. Serum CT increased (p < 0.001), while serum T and P were reduced (p < 0.01) in older rats. Serum Ca2+ was lower (p < 0.0001) in middle-aged rats compared to young adults. We revealed a 20% incidence of C-cell hyperplasia in older rats and one case of medullary thyroid carcinoma in an old rat. Our findings indicate that the ageing process causes significant histomorphometric changes at the thyroid C-cell level.",
publisher = "New York: Cambridge University Press",
journal = "Microscopy and Microanalysis",
title = "Age-Related Changes in Calcitonin-Producing Thyroid C-Cells of Male Wistar Rats",
doi = "10.1017/S1431927622000721"
}

Filipović, B., Ajdžanović, V., Živanović, J., Trifunović, S., Ristić, N., Milošević, V.,& Šošić-Jurjević, B.. (2022). Age-Related Changes in Calcitonin-Producing Thyroid C-Cells of Male Wistar Rats. in Microscopy and Microanalysis
New York: Cambridge University Press..
https://doi.org/10.1017/S1431927622000721

Filipović B, Ajdžanović V, Živanović J, Trifunović S, Ristić N, Milošević V, Šošić-Jurjević B. Age-Related Changes in Calcitonin-Producing Thyroid C-Cells of Male Wistar Rats. in Microscopy and Microanalysis. 2022;.
doi:10.1017/S1431927622000721 .

Filipović, Branko, Ajdžanović, Vladimir, Živanović, Jasmina, Trifunović, Svetlana, Ristić, Nataša, Milošević, Verica, Šošić-Jurjević, Branka , "Age-Related Changes in Calcitonin-Producing Thyroid C-Cells of Male Wistar Rats" in Microscopy and Microanalysis (2022),
https://doi.org/10.1017/S1431927622000721 . .

TY  - JOUR
AU  - Šošić-Jurjević, Branka 
AU  - Trifunović, Svetlana
AU  - Živanović, Jasmina
AU  - Ajdžanović, Vladimir
AU  - Miler, Marko
AU  - Ristić, Nataša
AU  - Filipović, Branko
PY  - 2022
UR  - https://www.mdpi.com/1422-0067/23/2/791
UR  - http://www.ncbi.nlm.nih.gov/pubmed/35054977
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4769
AB  - Vitamin D plays an essential role in prevention and treatment of osteoporosis. Thyroid hormones, in addition to vitamin D, significantly contribute to regulation of bone remodeling cycle and health. There is currently no data about a possible connection between vitamin D treatment and the thyroid in the context of osteoporosis. Middle-aged Wistar rats were divided into: sham operated (SO), orchidectomized (Orx), and cholecalciferol-treated orchidectomized (Orx + Vit. D3; 5 µg/kg b.m./day during three weeks) groups (n = 6/group). Concentration of 25(OH)D in serum of the Orx + Vit. D3 group increased 4 and 3.2 times (p < 0.0001) respectively, compared to Orx and SO group. T4, TSH, and calcitonin in serum remained unaltered. Vit. D3 treatment induced changes in thyroid functional morphology that indicate increased utilization of stored colloid and release of thyroid hormones in comparison with hormone synthesis, to maintain hormonal balance. Increased expression of nuclear VDR (p < 0.05) points to direct, TSH independent action of Vit. D on thyrocytes. Strong CYP24A1 immunostaining in C cells suggests its prominent expression in response to Vit. D in this cell subpopulation in orchidectomized rat model of osteoporosis. The indirect effect of Vit. D on bone, through fine regulation of thyroid function, is small.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Vitamin D3 Treatment Alters Thyroid Functional Morphology in Orchidectomized Rat Model of Osteoporosis.
IS  - 2
VL  - 23
DO  - 10.3390/ijms23020791
SP  - 791
ER  - 

@article{
author = "Šošić-Jurjević, Branka  and Trifunović, Svetlana and Živanović, Jasmina and Ajdžanović, Vladimir and Miler, Marko and Ristić, Nataša and Filipović, Branko",
year = "2022",
abstract = "Vitamin D plays an essential role in prevention and treatment of osteoporosis. Thyroid hormones, in addition to vitamin D, significantly contribute to regulation of bone remodeling cycle and health. There is currently no data about a possible connection between vitamin D treatment and the thyroid in the context of osteoporosis. Middle-aged Wistar rats were divided into: sham operated (SO), orchidectomized (Orx), and cholecalciferol-treated orchidectomized (Orx + Vit. D3; 5 µg/kg b.m./day during three weeks) groups (n = 6/group). Concentration of 25(OH)D in serum of the Orx + Vit. D3 group increased 4 and 3.2 times (p < 0.0001) respectively, compared to Orx and SO group. T4, TSH, and calcitonin in serum remained unaltered. Vit. D3 treatment induced changes in thyroid functional morphology that indicate increased utilization of stored colloid and release of thyroid hormones in comparison with hormone synthesis, to maintain hormonal balance. Increased expression of nuclear VDR (p < 0.05) points to direct, TSH independent action of Vit. D on thyrocytes. Strong CYP24A1 immunostaining in C cells suggests its prominent expression in response to Vit. D in this cell subpopulation in orchidectomized rat model of osteoporosis. The indirect effect of Vit. D on bone, through fine regulation of thyroid function, is small.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Vitamin D3 Treatment Alters Thyroid Functional Morphology in Orchidectomized Rat Model of Osteoporosis.",
number = "2",
volume = "23",
doi = "10.3390/ijms23020791",
pages = "791"
}

Šošić-Jurjević, B., Trifunović, S., Živanović, J., Ajdžanović, V., Miler, M., Ristić, N.,& Filipović, B.. (2022). Vitamin D3 Treatment Alters Thyroid Functional Morphology in Orchidectomized Rat Model of Osteoporosis.. in International Journal of Molecular Sciences
Basel: MDPI., 23(2), 791.
https://doi.org/10.3390/ijms23020791

Šošić-Jurjević B, Trifunović S, Živanović J, Ajdžanović V, Miler M, Ristić N, Filipović B. Vitamin D3 Treatment Alters Thyroid Functional Morphology in Orchidectomized Rat Model of Osteoporosis.. in International Journal of Molecular Sciences. 2022;23(2):791.
doi:10.3390/ijms23020791 .

Šošić-Jurjević, Branka , Trifunović, Svetlana, Živanović, Jasmina, Ajdžanović, Vladimir, Miler, Marko, Ristić, Nataša, Filipović, Branko, "Vitamin D3 Treatment Alters Thyroid Functional Morphology in Orchidectomized Rat Model of Osteoporosis." in International Journal of Molecular Sciences, 23, no. 2 (2022):791,
https://doi.org/10.3390/ijms23020791 . .

TY  - CONF
AU  - Ajduković, Maja
AU  - Vučić, Tijana
AU  - Cvijanović, Milena
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka 
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5227
AB  - Aquatic ecosystems receive the majority of global pollution. Therefore, aquatic
organisms are the most exposed and the most vulnerable as penetration of various
endocrine disruptors is easier, resulting in high bioavailability and bioaccumulation of
chemicals. One of the potent endocrine disruptors (anti-thyroid agents) is thiourea,
which chemically blocks the synthesis of thyroid hormones and prevents
metamorphosis in amphibians. The mechanism of action is very similar for all antithyroid agents via inhibition of thyroid peroxidase enzyme (TPO), thereby inhibiting
iodination of tyrosine residues in thyroglobulin, the oxidative coupling of iodinated
tyrosine and therefore the biosynthesis of the thyroid hormones. We investigated the
influence of two non-lethal concentrations of thiourea (0.05% and 0.1%) on
histomorphology of the thyroid gland in Triturus newts at the metamorphic stage,
when TH concentrations should reach maximum level. Exposure to thiourea induced
hypertrophy and hyperplasia of follicular cells as well as a significant reduction
(p<0.05) of interstitial tissue. Intensity of thyroglobulin immunostaining significantly
decreased (p<0.05) upon both thiourea treatments. Additionally, we tested
mammalian thyroglobulin antibodies for immunohistochemical assessment of the
thyroid follicular tissue in newts. Successful cross-reactivity of human primary
antibody in immunochemical detection of thyroglobulin in Triturus newts in this study
confirms potential homology in protein structure throughout the vertebrates.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
T1  - Changes in thyroid histomorphology and thyroglobulin immunostaining upon exposure to thiourea in Triturus newts
SP  - 41
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5227
ER  - 

@conference{
author = "Ajduković, Maja and Vučić, Tijana and Cvijanović, Milena and Filipović, Branko and Šošić-Jurjević, Branka ",
year = "2022",
abstract = "Aquatic ecosystems receive the majority of global pollution. Therefore, aquatic
organisms are the most exposed and the most vulnerable as penetration of various
endocrine disruptors is easier, resulting in high bioavailability and bioaccumulation of
chemicals. One of the potent endocrine disruptors (anti-thyroid agents) is thiourea,
which chemically blocks the synthesis of thyroid hormones and prevents
metamorphosis in amphibians. The mechanism of action is very similar for all antithyroid agents via inhibition of thyroid peroxidase enzyme (TPO), thereby inhibiting
iodination of tyrosine residues in thyroglobulin, the oxidative coupling of iodinated
tyrosine and therefore the biosynthesis of the thyroid hormones. We investigated the
influence of two non-lethal concentrations of thiourea (0.05% and 0.1%) on
histomorphology of the thyroid gland in Triturus newts at the metamorphic stage,
when TH concentrations should reach maximum level. Exposure to thiourea induced
hypertrophy and hyperplasia of follicular cells as well as a significant reduction
(p<0.05) of interstitial tissue. Intensity of thyroglobulin immunostaining significantly
decreased (p<0.05) upon both thiourea treatments. Additionally, we tested
mammalian thyroglobulin antibodies for immunohistochemical assessment of the
thyroid follicular tissue in newts. Successful cross-reactivity of human primary
antibody in immunochemical detection of thyroglobulin in Triturus newts in this study
confirms potential homology in protein structure throughout the vertebrates.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia",
title = "Changes in thyroid histomorphology and thyroglobulin immunostaining upon exposure to thiourea in Triturus newts",
pages = "41",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5227"
}

Ajduković, M., Vučić, T., Cvijanović, M., Filipović, B.,& Šošić-Jurjević, B.. (2022). Changes in thyroid histomorphology and thyroglobulin immunostaining upon exposure to thiourea in Triturus newts. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 41.
https://hdl.handle.net/21.15107/rcub_ibiss_5227

Ajduković M, Vučić T, Cvijanović M, Filipović B, Šošić-Jurjević B. Changes in thyroid histomorphology and thyroglobulin immunostaining upon exposure to thiourea in Triturus newts. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia. 2022;:41.
https://hdl.handle.net/21.15107/rcub_ibiss_5227 .

Ajduković, Maja, Vučić, Tijana, Cvijanović, Milena, Filipović, Branko, Šošić-Jurjević, Branka , "Changes in thyroid histomorphology and thyroglobulin immunostaining upon exposure to thiourea in Triturus newts" in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia (2022):41,
https://hdl.handle.net/21.15107/rcub_ibiss_5227 .

TY  - JOUR
AU  - Ajdžanović, Vladimir
AU  - Šošić-Jurjević, Branka 
AU  - Vodnik, Vesna
AU  - Filipović, Branko
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5076
PB  - Dortmund: Leibniz Research Centre for Working Environment and Human Factors, EXCLI Journal
T2  - EXCLI Journal
T1  - Interventions on soy isoflavone molecules to improve their therapeutic potential for prostate cancer treatment
VL  - 21
DO  - 10.17179/excli2022-5130
SP  - 941
EP  - 947
ER  - 

@article{
author = "Ajdžanović, Vladimir and Šošić-Jurjević, Branka  and Vodnik, Vesna and Filipović, Branko",
year = "2022",
publisher = "Dortmund: Leibniz Research Centre for Working Environment and Human Factors, EXCLI Journal",
journal = "EXCLI Journal",
title = "Interventions on soy isoflavone molecules to improve their therapeutic potential for prostate cancer treatment",
volume = "21",
doi = "10.17179/excli2022-5130",
pages = "941-947"
}

Ajdžanović, V., Šošić-Jurjević, B., Vodnik, V.,& Filipović, B.. (2022). Interventions on soy isoflavone molecules to improve their therapeutic potential for prostate cancer treatment. in EXCLI Journal
Dortmund: Leibniz Research Centre for Working Environment and Human Factors, EXCLI Journal., 21, 941-947.
https://doi.org/10.17179/excli2022-5130

Ajdžanović V, Šošić-Jurjević B, Vodnik V, Filipović B. Interventions on soy isoflavone molecules to improve their therapeutic potential for prostate cancer treatment. in EXCLI Journal. 2022;21:941-947.
doi:10.17179/excli2022-5130 .

Ajdžanović, Vladimir, Šošić-Jurjević, Branka , Vodnik, Vesna, Filipović, Branko, "Interventions on soy isoflavone molecules to improve their therapeutic potential for prostate cancer treatment" in EXCLI Journal, 21 (2022):941-947,
https://doi.org/10.17179/excli2022-5130 . .

TY  - CONF
AU  - Miler, Marko
AU  - Živanović, Jasmina
AU  - Ristić, Nataša
AU  - Manojlović-Stojanoski, Milica
AU  - Trifunović, Svetlana
AU  - Šošić-Jurjević, Branka 
AU  - Nestorović, Nataša
AU  - Filipović, Branko
AU  - Ajdžanović, Vladimir
PY  - 2022
UR  - https://www.serbiosoc.org.rs/?page_id=702
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6034
AB  - Старење доводи до акумулације и смањене елиминације реактивних врста,
стварајући оксидациону средину у јетри. Једна од могућих опција за одлагање овог
догађаја је употреба антиоксиданата из цитруса у склопу процеса здравог старења.
Стога, ова студија je имала за циљ да истражи ефекте ериоцитрина (ЕРИ),
полифенола из лимуна, на редокс средину у јетри код 24-месечних мужјака Wistar
пацова. ЕРИ (30 мг/кг т.м.) је даван орално, једном дневно током четири недеље.
Контролне групе су примиле или носач третмана (сунцокретово уље) или су остале
интактне. Примењена методологија подразумевала је имуноблот и qPCR анализу,
детекцију нивоа персулфидације у гелу и биохемијске анализе. Третман ЕРИ
повећао је експресију гена и протеина за нуклеарни фактор 2 (Nrf2), тиоредоксин
(Trx) 1, глутатион пероксидазу и редуктазу. Експресија гена супероксид дисмутазе
2 (SOD2) и Trx2 је смањена, док је примена ЕРИ повећала експресију протеина
SOD2. Штавише, ЕРИ је смањио концентрацију малондиалдехида, маркера
оксидативног оштећења у ћелији. Показали смо смањење персулфидације протеина
у јетри након третмана ЕРИ, док су генска и протеинска експресија ензима који
производе H2S остале непромењене. У закључку, ЕРИ појачава регулацију редокс
регулатора јетре Nrf2, који активира антиоксидативни одбрамбени систем и Trx1,
што доводи до смањења нивоа персулфидације протеина у јетри. Наши налази
указују на потенцијал ЕРИ у унапређењу редокс средине у јетри старих пацова.
AB  - Starenje dovodi do akumulacije i smanjene eliminacije reaktivnih vrsta,
stvarajući oksidacionu sredinu u jetri. Jedna od mogućih opcija za odlaganje ovog
događaja je upotreba antioksidanata iz citrusa u sklopu procesa zdravog starenja.
Stoga, ova studija je imala za cilj da istraži efekte eriocitrina (ERI),
polifenola iz limuna, na redoks sredinu u jetri kod 24-mesečnih mužjaka Wistar
pacova. ERI (30 mg/kg t.m.) je davan oralno, jednom dnevno tokom četiri nedelje.
Kontrolne grupe su primile ili nosač tretmana (suncokretovo ulje) ili su ostale
intaktne. Primenjena metodologija podrazumevala je imunoblot i qPCR analizu,
detekciju nivoa persulfidacije u gelu i biohemijske analize. Tretman ERI
povećao je ekspresiju gena i proteina za nuklearni faktor 2 (Nrf2), tioredoksin
(Trx) 1, glutation peroksidazu i reduktazu. Ekspresija gena superoksid dismutaze
2 (SOD2) i Trx2 je smanjena, dok je primena ERI povećala ekspresiju proteina
SOD2. Štaviše, ERI je smanjio koncentraciju malondialdehida, markera
oksidativnog oštećenja u ćeliji. Pokazali smo smanjenje persulfidacije proteina
u jetri nakon tretmana ERI, dok su genska i proteinska ekspresija enzima koji
proizvode H2S ostale nepromenjene. U zaključku, ERI pojačava regulaciju redoks
regulatora jetre Nrf2, koji aktivira antioksidativni odbrambeni sistem i Trx1,
što dovodi do smanjenja nivoa persulfidacije proteina u jetri. Naši nalazi
ukazuju na potencijal ERI u unapređenju redoks sredine u jetri starih pacova.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Eриоцитрин, полифенол из лимуна, унапређује редокс средину у јетри старих пацова
T1  - Eriocitrin, polifenol iz limuna, unapređuje redoks sredinu u jetri starih pacova
SP  - 374
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6034
ER  - 

@conference{
author = "Miler, Marko and Živanović, Jasmina and Ristić, Nataša and Manojlović-Stojanoski, Milica and Trifunović, Svetlana and Šošić-Jurjević, Branka  and Nestorović, Nataša and Filipović, Branko and Ajdžanović, Vladimir",
year = "2022",
abstract = "Старење доводи до акумулације и смањене елиминације реактивних врста,
стварајући оксидациону средину у јетри. Једна од могућих опција за одлагање овог
догађаја је употреба антиоксиданата из цитруса у склопу процеса здравог старења.
Стога, ова студија je имала за циљ да истражи ефекте ериоцитрина (ЕРИ),
полифенола из лимуна, на редокс средину у јетри код 24-месечних мужјака Wistar
пацова. ЕРИ (30 мг/кг т.м.) је даван орално, једном дневно током четири недеље.
Контролне групе су примиле или носач третмана (сунцокретово уље) или су остале
интактне. Примењена методологија подразумевала је имуноблот и qPCR анализу,
детекцију нивоа персулфидације у гелу и биохемијске анализе. Третман ЕРИ
повећао је експресију гена и протеина за нуклеарни фактор 2 (Nrf2), тиоредоксин
(Trx) 1, глутатион пероксидазу и редуктазу. Експресија гена супероксид дисмутазе
2 (SOD2) и Trx2 је смањена, док је примена ЕРИ повећала експресију протеина
SOD2. Штавише, ЕРИ је смањио концентрацију малондиалдехида, маркера
оксидативног оштећења у ћелији. Показали смо смањење персулфидације протеина
у јетри након третмана ЕРИ, док су генска и протеинска експресија ензима који
производе H2S остале непромењене. У закључку, ЕРИ појачава регулацију редокс
регулатора јетре Nrf2, који активира антиоксидативни одбрамбени систем и Trx1,
што доводи до смањења нивоа персулфидације протеина у јетри. Наши налази
указују на потенцијал ЕРИ у унапређењу редокс средине у јетри старих пацова., Starenje dovodi do akumulacije i smanjene eliminacije reaktivnih vrsta,
stvarajući oksidacionu sredinu u jetri. Jedna od mogućih opcija za odlaganje ovog
događaja je upotreba antioksidanata iz citrusa u sklopu procesa zdravog starenja.
Stoga, ova studija je imala za cilj da istraži efekte eriocitrina (ERI),
polifenola iz limuna, na redoks sredinu u jetri kod 24-mesečnih mužjaka Wistar
pacova. ERI (30 mg/kg t.m.) je davan oralno, jednom dnevno tokom četiri nedelje.
Kontrolne grupe su primile ili nosač tretmana (suncokretovo ulje) ili su ostale
intaktne. Primenjena metodologija podrazumevala je imunoblot i qPCR analizu,
detekciju nivoa persulfidacije u gelu i biohemijske analize. Tretman ERI
povećao je ekspresiju gena i proteina za nuklearni faktor 2 (Nrf2), tioredoksin
(Trx) 1, glutation peroksidazu i reduktazu. Ekspresija gena superoksid dismutaze
2 (SOD2) i Trx2 je smanjena, dok je primena ERI povećala ekspresiju proteina
SOD2. Štaviše, ERI je smanjio koncentraciju malondialdehida, markera
oksidativnog oštećenja u ćeliji. Pokazali smo smanjenje persulfidacije proteina
u jetri nakon tretmana ERI, dok su genska i proteinska ekspresija enzima koji
proizvode H2S ostale nepromenjene. U zaključku, ERI pojačava regulaciju redoks
regulatora jetre Nrf2, koji aktivira antioksidativni odbrambeni sistem i Trx1,
što dovodi do smanjenja nivoa persulfidacije proteina u jetri. Naši nalazi
ukazuju na potencijal ERI u unapređenju redoks sredine u jetri starih pacova.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Eриоцитрин, полифенол из лимуна, унапређује редокс средину у јетри старих пацова, Eriocitrin, polifenol iz limuna, unapređuje redoks sredinu u jetri starih pacova",
pages = "374",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6034"
}

Miler, M., Živanović, J., Ristić, N., Manojlović-Stojanoski, M., Trifunović, S., Šošić-Jurjević, B., Nestorović, N., Filipović, B.,& Ajdžanović, V.. (2022). Eриоцитрин, полифенол из лимуна, унапређује редокс средину у јетри старих пацова. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 374.
https://hdl.handle.net/21.15107/rcub_ibiss_6034

Miler M, Živanović J, Ristić N, Manojlović-Stojanoski M, Trifunović S, Šošić-Jurjević B, Nestorović N, Filipović B, Ajdžanović V. Eриоцитрин, полифенол из лимуна, унапређује редокс средину у јетри старих пацова. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:374.
https://hdl.handle.net/21.15107/rcub_ibiss_6034 .

Miler, Marko, Živanović, Jasmina, Ristić, Nataša, Manojlović-Stojanoski, Milica, Trifunović, Svetlana, Šošić-Jurjević, Branka , Nestorović, Nataša, Filipović, Branko, Ajdžanović, Vladimir, "Eриоцитрин, полифенол из лимуна, унапређује редокс средину у јетри старих пацова" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):374,
https://hdl.handle.net/21.15107/rcub_ibiss_6034 .

TY  - CONF
AU  - Filipović, Branko
AU  - Ajdžanović, Vladimir
AU  - Živanović, Jasmina
AU  - Manojlović-Stojanoski, Milica
AU  - Trifunović, Svetlana
AU  - Nestorović, Nataša
AU  - Šošić-Jurjević, Branka 
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4976
AB  - Thyroid C-cells, as a second type of endocrine cell population within thyroid, produce and secrete peptide hormone calcitonin (CT). This hypocalcemic hormone acts as an inhibitor of bone resorption. The aging is a complex process that alters various cellular functions. Therefore, the aim of this study was to examine the aging-related changes in the structure and function of CT-producing thyroid C-cells in male Wistar rats, using histomorphometric, ultrastructural and biochemical analysis. The investigation was performed on three groups of male rats: adult (3-months old), middle-aged (16-months old) and old (24-months old). The peroxidase-antiperoxidase method was applied for localization of CT in the C-cells. Stereological analysis was performed using Olimpus microscope (BX-51), equipped with a microcator, a motorised stage and a CCD video camera, and controlled by the newCAST stereological software package. Blood serum samples were analyzed for determination of CT, testosterone (T), calcium (Ca2+) and phosphorus (P) concentrations. We found a significant increase in the volume density (Vv) of thyroid C-cells in both middle-aged and old rats, all compared to adult animals. The percentage of smaller volume range C-cells (<500 μm3) increases, while the proportion of greater volume rang C-cells (both 500-1000 μm3 and >1000 μm3) markedly decreases with aging. By ultrastructural analysis we found that the average number of secretory granules per C-cell was significantly increased in both middle-aged and aged rats, all compared to adults. Unlike the C-cells of adult rats, these granules in older, especially in old animals, had a content of fairly low density. By the biochemical analysis, we detected a significant increase in serum CT levels, while serum T was markedly reduced in both middle aged and old rats, all in comparison with adults. Serum Ca2+ concentration significantly decreased in middle-aged rats compared to adults, while concentration of serum Pwas lower in both middle-aged and old rats, all related to adult group. Our findings show that aging process increases the Vv of thyroid C-cells, with a simultaneous change in percentage of cells with larger and smaller volume rang, and an increase in the number of both cell types. These changes, accompanied by modulation of the cellular ultrastructure and an increase in serum CT levels, reflect the structure and function of CT-producing thyroid C-cells in our aging model.
PB  - bioscientifica
C3  - European Congress of Endocrinology 2022; 2022 May 21-24; Milan, Italy
T1  - The effects of aging on thyroid C-cells in male rats
DO  - 10.1530/endoabs.81.P477
SP  - P477
ER  - 

@conference{
author = "Filipović, Branko and Ajdžanović, Vladimir and Živanović, Jasmina and Manojlović-Stojanoski, Milica and Trifunović, Svetlana and Nestorović, Nataša and Šošić-Jurjević, Branka ",
year = "2022",
abstract = "Thyroid C-cells, as a second type of endocrine cell population within thyroid, produce and secrete peptide hormone calcitonin (CT). This hypocalcemic hormone acts as an inhibitor of bone resorption. The aging is a complex process that alters various cellular functions. Therefore, the aim of this study was to examine the aging-related changes in the structure and function of CT-producing thyroid C-cells in male Wistar rats, using histomorphometric, ultrastructural and biochemical analysis. The investigation was performed on three groups of male rats: adult (3-months old), middle-aged (16-months old) and old (24-months old). The peroxidase-antiperoxidase method was applied for localization of CT in the C-cells. Stereological analysis was performed using Olimpus microscope (BX-51), equipped with a microcator, a motorised stage and a CCD video camera, and controlled by the newCAST stereological software package. Blood serum samples were analyzed for determination of CT, testosterone (T), calcium (Ca2+) and phosphorus (P) concentrations. We found a significant increase in the volume density (Vv) of thyroid C-cells in both middle-aged and old rats, all compared to adult animals. The percentage of smaller volume range C-cells (<500 μm3) increases, while the proportion of greater volume rang C-cells (both 500-1000 μm3 and >1000 μm3) markedly decreases with aging. By ultrastructural analysis we found that the average number of secretory granules per C-cell was significantly increased in both middle-aged and aged rats, all compared to adults. Unlike the C-cells of adult rats, these granules in older, especially in old animals, had a content of fairly low density. By the biochemical analysis, we detected a significant increase in serum CT levels, while serum T was markedly reduced in both middle aged and old rats, all in comparison with adults. Serum Ca2+ concentration significantly decreased in middle-aged rats compared to adults, while concentration of serum Pwas lower in both middle-aged and old rats, all related to adult group. Our findings show that aging process increases the Vv of thyroid C-cells, with a simultaneous change in percentage of cells with larger and smaller volume rang, and an increase in the number of both cell types. These changes, accompanied by modulation of the cellular ultrastructure and an increase in serum CT levels, reflect the structure and function of CT-producing thyroid C-cells in our aging model.",
publisher = "bioscientifica",
journal = "European Congress of Endocrinology 2022; 2022 May 21-24; Milan, Italy",
title = "The effects of aging on thyroid C-cells in male rats",
doi = "10.1530/endoabs.81.P477",
pages = "P477"
}

Filipović, B., Ajdžanović, V., Živanović, J., Manojlović-Stojanoski, M., Trifunović, S., Nestorović, N.,& Šošić-Jurjević, B.. (2022). The effects of aging on thyroid C-cells in male rats. in European Congress of Endocrinology 2022; 2022 May 21-24; Milan, Italy
bioscientifica., P477.
https://doi.org/10.1530/endoabs.81.P477

Filipović B, Ajdžanović V, Živanović J, Manojlović-Stojanoski M, Trifunović S, Nestorović N, Šošić-Jurjević B. The effects of aging on thyroid C-cells in male rats. in European Congress of Endocrinology 2022; 2022 May 21-24; Milan, Italy. 2022;:P477.
doi:10.1530/endoabs.81.P477 .

Filipović, Branko, Ajdžanović, Vladimir, Živanović, Jasmina, Manojlović-Stojanoski, Milica, Trifunović, Svetlana, Nestorović, Nataša, Šošić-Jurjević, Branka , "The effects of aging on thyroid C-cells in male rats" in European Congress of Endocrinology 2022; 2022 May 21-24; Milan, Italy (2022):P477,
https://doi.org/10.1530/endoabs.81.P477 . .

TY  - CONF
AU  - Šošić-Jurjević, Branka 
AU  - Jovanović, Ljubiša
AU  - Marina, Ljiljana
AU  - Ristić, Nataša
AU  - Miler, Marko
AU  - Ajdžanović, Vladimir
AU  - Filipović, Branko
AU  - Luetjohann, Dieter
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4975
AB  - The cholesterol oxidation product 27-hydroxycholesterol (27OHC) is enzymatically
produced from cholesterol by CYP27A1 in an alternative pathway of
cholesterol degradation to bile acids. This oxysterol also acts as an endogenous
selective estrogen receptor modulator (SERM). In healthy humans its
concentration in circulation increases in hypercholesterolemia and with age,
and is associated with increased risk of atherosclerosis, cardiovascular diseases
and breast cancer. Several drugs with SERM activity used for treatments of breast
cancer or osteoporosis have been reported to have sporadic hepatotoxic effects.
Women suffer from some liver diseases more commonly (acute liver failure,
autoimmune hepatitis, benign liver lesions, or primary biliary cirrhosis). For all of
these the incidence increases with advancing age. To the best of our knowledge,
there is no information in the literature, clinical or experimental, relating changes
in hepatic 27OHC with incidence of liver disease in the context of aging and sex.
To address this problem, we examined the effect of age and sex on liver and serum
concentrations of 27OHC, as well as the immunostaining pattern of CYP27A1 in
the liver of four-month and 24-month-old Wistar rats (experiments were repeated
twice with similar results, nZ5-6 animals/group) using LC MS/MS and
immunohistochemistry, respectively. Furthermore, we examined changes in
total cholesterol and concentration in liver and serum, liver histopathology, as
well as serum concentration of hepatic enzymes, alanine (ALT) and aspartate
aminotransferase (AST). The effect of age (P!0.05) on increase of serum and
hepatic 27OHC was obtained both in males and females (P!0.05) and followed
the same pattern of age-related total cholesterol increase (P!0.05). However, the
intrahepatic increase of 27OHC was dramatically more pronounced only in oldaged
females (P!0.0001). CYP27A1 immunostaining intensity was similar in all
experimental groups, being the strongest in the cytoplasm of centrilobular
hepatocytes, but the immunopositivity was diffusely spread throughout the liver
lobule. Histopathological analysis revealed age-related hepatocellular degeneration
(swelling and hydropic degeneration, increased fraction of binuclear
hepatocytes and focal fatty changes) only in females. Moreover, age-related
elevation of alanine transaminase (ALT) was observed only in female rats (P!
0.01). In conclusion, the obtained results confirmed age-related female-specific
increase of hepatic 27OHC as well as hepatocyte degeneration obsereved only in
the liver of rat females. These age-related adaptive changes in cholesterol
metabolism may atenuate hepatoprotective estrogen-like effects in the liver.
PB  - bioscientifica
C3  - 24th European Congress of  Endocrinology 2022; 21–24 May 2022; Milan, Italy
T1  - Is age-related hepatic elevation of endogenous SERM 27-hydroxycho lesterol associated with hepatocellular degeneration female-specific? - Results from Rat study?
DO  - 10.1530/endoabs.81.P615
SP  - P615
ER  - 

@conference{
author = "Šošić-Jurjević, Branka  and Jovanović, Ljubiša and Marina, Ljiljana and Ristić, Nataša and Miler, Marko and Ajdžanović, Vladimir and Filipović, Branko and Luetjohann, Dieter",
year = "2022",
abstract = "The cholesterol oxidation product 27-hydroxycholesterol (27OHC) is enzymatically
produced from cholesterol by CYP27A1 in an alternative pathway of
cholesterol degradation to bile acids. This oxysterol also acts as an endogenous
selective estrogen receptor modulator (SERM). In healthy humans its
concentration in circulation increases in hypercholesterolemia and with age,
and is associated with increased risk of atherosclerosis, cardiovascular diseases
and breast cancer. Several drugs with SERM activity used for treatments of breast
cancer or osteoporosis have been reported to have sporadic hepatotoxic effects.
Women suffer from some liver diseases more commonly (acute liver failure,
autoimmune hepatitis, benign liver lesions, or primary biliary cirrhosis). For all of
these the incidence increases with advancing age. To the best of our knowledge,
there is no information in the literature, clinical or experimental, relating changes
in hepatic 27OHC with incidence of liver disease in the context of aging and sex.
To address this problem, we examined the effect of age and sex on liver and serum
concentrations of 27OHC, as well as the immunostaining pattern of CYP27A1 in
the liver of four-month and 24-month-old Wistar rats (experiments were repeated
twice with similar results, nZ5-6 animals/group) using LC MS/MS and
immunohistochemistry, respectively. Furthermore, we examined changes in
total cholesterol and concentration in liver and serum, liver histopathology, as
well as serum concentration of hepatic enzymes, alanine (ALT) and aspartate
aminotransferase (AST). The effect of age (P!0.05) on increase of serum and
hepatic 27OHC was obtained both in males and females (P!0.05) and followed
the same pattern of age-related total cholesterol increase (P!0.05). However, the
intrahepatic increase of 27OHC was dramatically more pronounced only in oldaged
females (P!0.0001). CYP27A1 immunostaining intensity was similar in all
experimental groups, being the strongest in the cytoplasm of centrilobular
hepatocytes, but the immunopositivity was diffusely spread throughout the liver
lobule. Histopathological analysis revealed age-related hepatocellular degeneration
(swelling and hydropic degeneration, increased fraction of binuclear
hepatocytes and focal fatty changes) only in females. Moreover, age-related
elevation of alanine transaminase (ALT) was observed only in female rats (P!
0.01). In conclusion, the obtained results confirmed age-related female-specific
increase of hepatic 27OHC as well as hepatocyte degeneration obsereved only in
the liver of rat females. These age-related adaptive changes in cholesterol
metabolism may atenuate hepatoprotective estrogen-like effects in the liver.",
publisher = "bioscientifica",
journal = "24th European Congress of  Endocrinology 2022; 21–24 May 2022; Milan, Italy",
title = "Is age-related hepatic elevation of endogenous SERM 27-hydroxycho lesterol associated with hepatocellular degeneration female-specific? - Results from Rat study?",
doi = "10.1530/endoabs.81.P615",
pages = "P615"
}

Šošić-Jurjević, B., Jovanović, L., Marina, L., Ristić, N., Miler, M., Ajdžanović, V., Filipović, B.,& Luetjohann, D.. (2022). Is age-related hepatic elevation of endogenous SERM 27-hydroxycho lesterol associated with hepatocellular degeneration female-specific? - Results from Rat study?. in 24th European Congress of  Endocrinology 2022; 21–24 May 2022; Milan, Italy
bioscientifica., P615.
https://doi.org/10.1530/endoabs.81.P615

Šošić-Jurjević B, Jovanović L, Marina L, Ristić N, Miler M, Ajdžanović V, Filipović B, Luetjohann D. Is age-related hepatic elevation of endogenous SERM 27-hydroxycho lesterol associated with hepatocellular degeneration female-specific? - Results from Rat study?. in 24th European Congress of  Endocrinology 2022; 21–24 May 2022; Milan, Italy. 2022;:P615.
doi:10.1530/endoabs.81.P615 .

Šošić-Jurjević, Branka , Jovanović, Ljubiša, Marina, Ljiljana, Ristić, Nataša, Miler, Marko, Ajdžanović, Vladimir, Filipović, Branko, Luetjohann, Dieter, "Is age-related hepatic elevation of endogenous SERM 27-hydroxycho lesterol associated with hepatocellular degeneration female-specific? - Results from Rat study?" in 24th European Congress of  Endocrinology 2022; 21–24 May 2022; Milan, Italy (2022):P615,
https://doi.org/10.1530/endoabs.81.P615 . .

TY  - JOUR
AU  - Ajdžanović, Vladimir
AU  - Miler, Marko
AU  - Šošić-Jurjević, Branka 
AU  - Filipović, Branko
AU  - Milenković, Dragan
AU  - Jakovljević, Vladimir
AU  - Milošević, Verica
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4144
AB  - In a series of our previous works, we revealed the beneficial effects of applied soy isoflavones (genistein or
daidzein) on the wide context of corticosteroidogenesis in vivo, in a rat model of the andropause. Soy isoflavones
decreased the circulating levels of pituitary adrenocorticotropic hormone, inhibited aldosterone secretion, as
well as corticosterone production and secretion, but stimulated dehydroepiandrosterone secretion, all in
andropausal rats. In vitro studies indicate that the mechanism underlying these hormonal changes relies on inhibition of the pituitary tyrosine kinase and adrenocortical 3β-hydroxysteroid dehydrogenase enzymes by soy
isoflavones. Although the clinical studies are in their infancy, the opinion is that genistein and daidzein have
therapeutic potential for the safe treatment of ageing-caused androgen deprivation and glucocorticoid excess
with related metabolic/hemodynamic issues in males. Our accumulated experience and knowledge in the field of
biomedical effects of plant polyphenols have provided a platform for potential recommending the agenda to
organize and accelerate experimental research aimed at producing the optimal supplementation. We hypothesize
that an in vivo approach should first be exploited in the sequence of investigative steps, followed by in vitro
studies and synchronously conducted molecular docking analyses. In vivo research, besides establishing the
margin of exposure safety or adjustment of the correct polyphenol dose, enables identification and quantification
of the metabolites of applied polyphenols in the blood. Subsequent in vitro exploitation of the metabolites and
related docking analyses provide clarification of the molecular mechanisms of action of applied polyphenols.
Chemical modification of the polyphenol structure or coupling it with nanoparticles might be the next step in
optimizing the design of supplementation. Selected, intact or chemically-modified polyphenol molecules should
be included in preclinical studies on a more closely-related species, while clinical studies would finally assess the
safety and effectiveness of a polyphenol-based remedial strategy. The final supplement represents a product of an
appropriate technological process, conducted in accordance with the recommendations derived from the preceding research.
PB  - Elsevir
T2  - Medical Hypotheses
T1  - Soy isoflavone-caused shunting of the corticosteroidogenesis pathways in andropausal subjects: Top-down impulse for the optimal supplementation design
VL  - 148
DO  - 10.1016/j.mehy.2021.110516
SP  - 110516
ER  - 

@article{
author = "Ajdžanović, Vladimir and Miler, Marko and Šošić-Jurjević, Branka  and Filipović, Branko and Milenković, Dragan and Jakovljević, Vladimir and Milošević, Verica",
year = "2021",
abstract = "In a series of our previous works, we revealed the beneficial effects of applied soy isoflavones (genistein or
daidzein) on the wide context of corticosteroidogenesis in vivo, in a rat model of the andropause. Soy isoflavones
decreased the circulating levels of pituitary adrenocorticotropic hormone, inhibited aldosterone secretion, as
well as corticosterone production and secretion, but stimulated dehydroepiandrosterone secretion, all in
andropausal rats. In vitro studies indicate that the mechanism underlying these hormonal changes relies on inhibition of the pituitary tyrosine kinase and adrenocortical 3β-hydroxysteroid dehydrogenase enzymes by soy
isoflavones. Although the clinical studies are in their infancy, the opinion is that genistein and daidzein have
therapeutic potential for the safe treatment of ageing-caused androgen deprivation and glucocorticoid excess
with related metabolic/hemodynamic issues in males. Our accumulated experience and knowledge in the field of
biomedical effects of plant polyphenols have provided a platform for potential recommending the agenda to
organize and accelerate experimental research aimed at producing the optimal supplementation. We hypothesize
that an in vivo approach should first be exploited in the sequence of investigative steps, followed by in vitro
studies and synchronously conducted molecular docking analyses. In vivo research, besides establishing the
margin of exposure safety or adjustment of the correct polyphenol dose, enables identification and quantification
of the metabolites of applied polyphenols in the blood. Subsequent in vitro exploitation of the metabolites and
related docking analyses provide clarification of the molecular mechanisms of action of applied polyphenols.
Chemical modification of the polyphenol structure or coupling it with nanoparticles might be the next step in
optimizing the design of supplementation. Selected, intact or chemically-modified polyphenol molecules should
be included in preclinical studies on a more closely-related species, while clinical studies would finally assess the
safety and effectiveness of a polyphenol-based remedial strategy. The final supplement represents a product of an
appropriate technological process, conducted in accordance with the recommendations derived from the preceding research.",
publisher = "Elsevir",
journal = "Medical Hypotheses",
title = "Soy isoflavone-caused shunting of the corticosteroidogenesis pathways in andropausal subjects: Top-down impulse for the optimal supplementation design",
volume = "148",
doi = "10.1016/j.mehy.2021.110516",
pages = "110516"
}

Ajdžanović, V., Miler, M., Šošić-Jurjević, B., Filipović, B., Milenković, D., Jakovljević, V.,& Milošević, V.. (2021). Soy isoflavone-caused shunting of the corticosteroidogenesis pathways in andropausal subjects: Top-down impulse for the optimal supplementation design. in Medical Hypotheses
Elsevir., 148, 110516.
https://doi.org/10.1016/j.mehy.2021.110516

Ajdžanović V, Miler M, Šošić-Jurjević B, Filipović B, Milenković D, Jakovljević V, Milošević V. Soy isoflavone-caused shunting of the corticosteroidogenesis pathways in andropausal subjects: Top-down impulse for the optimal supplementation design. in Medical Hypotheses. 2021;148:110516.
doi:10.1016/j.mehy.2021.110516 .

Ajdžanović, Vladimir, Miler, Marko, Šošić-Jurjević, Branka , Filipović, Branko, Milenković, Dragan, Jakovljević, Vladimir, Milošević, Verica, "Soy isoflavone-caused shunting of the corticosteroidogenesis pathways in andropausal subjects: Top-down impulse for the optimal supplementation design" in Medical Hypotheses, 148 (2021):110516,
https://doi.org/10.1016/j.mehy.2021.110516 . .

TY  - JOUR
AU  - Dejanović, Bratislav
AU  - Begović-Kuprešanin, Vesna
AU  - Stevanović, Ivana
AU  - Lavrnja, Irena
AU  - Šošić-Jurjević, Branka 
AU  - Ninković, Milica
AU  - Trifunović, Svetlana
PY  - 2021
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46642100028D
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4651
UR  - https://www.serbiosoc.org.rs/arch/index.php/abs/article/view/6557
AB  - The use of the antidepressant drug chlorpromazine (CPZ) is linked to the occurrence of oxidative stress in some brain structures. Thus, overcoming the side effects of CPZ is of great importance. Because agmatine (AGM) can act as a free radical scavenger, it is an interesting compound as an adjunct to CPZ therapy. The aim of our study was to investigate the enzymatic parameters of oxidative stress in the hippocampus and striatum of rats after CPZ treatment, and the potential protective effects of AGM. Rats were injected as follows with (i) 1 mL/kg b.w. saline; (ii) a single intraperitoneal (i.p.) dose of CPZ (38.7 mg/kg); (iii) CPZ (38.7 mg/kg) and AGM (75 mg/kg); (iv) AGM (75 mg/kg). CPZ induced an increase in superoxide anion radical (O2 catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR), were lowered in both the hippocampus striatum. Cotreatment with CPZ and AGM protected the examined brain structures by reversing the antioxidant enzyme control values. Following CPZ treatment, the effects were more pronounced for SOD and GPx in the hippocampus, the striatum. The full effect of restored superoxide production was achieved in the striatum, which points to the role of CAT. The obtained results suggest that CPZ in combination with AGM may be considered as a new treatment strategy.
PB  - Belgrade: Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - Agmatine reduces chlorpromazine prooxidant effects in rat hippocampus and striatum
IS  - 3
VL  - 73
DO  - 10.2298/abs210429028d
SP  - 353
EP  - 359
ER  - 

@article{
author = "Dejanović, Bratislav and Begović-Kuprešanin, Vesna and Stevanović, Ivana and Lavrnja, Irena and Šošić-Jurjević, Branka  and Ninković, Milica and Trifunović, Svetlana",
year = "2021",
abstract = "The use of the antidepressant drug chlorpromazine (CPZ) is linked to the occurrence of oxidative stress in some brain structures. Thus, overcoming the side effects of CPZ is of great importance. Because agmatine (AGM) can act as a free radical scavenger, it is an interesting compound as an adjunct to CPZ therapy. The aim of our study was to investigate the enzymatic parameters of oxidative stress in the hippocampus and striatum of rats after CPZ treatment, and the potential protective effects of AGM. Rats were injected as follows with (i) 1 mL/kg b.w. saline; (ii) a single intraperitoneal (i.p.) dose of CPZ (38.7 mg/kg); (iii) CPZ (38.7 mg/kg) and AGM (75 mg/kg); (iv) AGM (75 mg/kg). CPZ induced an increase in superoxide anion radical (O2 catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR), were lowered in both the hippocampus striatum. Cotreatment with CPZ and AGM protected the examined brain structures by reversing the antioxidant enzyme control values. Following CPZ treatment, the effects were more pronounced for SOD and GPx in the hippocampus, the striatum. The full effect of restored superoxide production was achieved in the striatum, which points to the role of CAT. The obtained results suggest that CPZ in combination with AGM may be considered as a new treatment strategy.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "Agmatine reduces chlorpromazine prooxidant effects in rat hippocampus and striatum",
number = "3",
volume = "73",
doi = "10.2298/abs210429028d",
pages = "353-359"
}

Dejanović, B., Begović-Kuprešanin, V., Stevanović, I., Lavrnja, I., Šošić-Jurjević, B., Ninković, M.,& Trifunović, S.. (2021). Agmatine reduces chlorpromazine prooxidant effects in rat hippocampus and striatum. in Archives of Biological Sciences
Belgrade: Serbian Biological Society., 73(3), 353-359.
https://doi.org/10.2298/abs210429028d

Dejanović B, Begović-Kuprešanin V, Stevanović I, Lavrnja I, Šošić-Jurjević B, Ninković M, Trifunović S. Agmatine reduces chlorpromazine prooxidant effects in rat hippocampus and striatum. in Archives of Biological Sciences. 2021;73(3):353-359.
doi:10.2298/abs210429028d .

Dejanović, Bratislav, Begović-Kuprešanin, Vesna, Stevanović, Ivana, Lavrnja, Irena, Šošić-Jurjević, Branka , Ninković, Milica, Trifunović, Svetlana, "Agmatine reduces chlorpromazine prooxidant effects in rat hippocampus and striatum" in Archives of Biological Sciences, 73, no. 3 (2021):353-359,
https://doi.org/10.2298/abs210429028d . .

TY  - JOUR
AU  - Ajdžanović, Vladimir
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka 
AU  - Miler, Marko
AU  - Milošević, Verica
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4223
PB  - Dortmund : IfADo - Leibniz Research Centre for Working Environment and Human Factors
T2  - EXCLI Journal
T1  - Margins of beneficial daily dosage of supplements in prevention of COVID-19
VL  - 20
DO  - 10.17179/excli2021-3790
SP  - 828
EP  - 834
ER  - 

@article{
author = "Ajdžanović, Vladimir and Filipović, Branko and Šošić-Jurjević, Branka  and Miler, Marko and Milošević, Verica",
year = "2021",
publisher = "Dortmund : IfADo - Leibniz Research Centre for Working Environment and Human Factors",
journal = "EXCLI Journal",
title = "Margins of beneficial daily dosage of supplements in prevention of COVID-19",
volume = "20",
doi = "10.17179/excli2021-3790",
pages = "828-834"
}

Ajdžanović, V., Filipović, B., Šošić-Jurjević, B., Miler, M.,& Milošević, V.. (2021). Margins of beneficial daily dosage of supplements in prevention of COVID-19. in EXCLI Journal
Dortmund : IfADo - Leibniz Research Centre for Working Environment and Human Factors., 20, 828-834.
https://doi.org/10.17179/excli2021-3790

Ajdžanović V, Filipović B, Šošić-Jurjević B, Miler M, Milošević V. Margins of beneficial daily dosage of supplements in prevention of COVID-19. in EXCLI Journal. 2021;20:828-834.
doi:10.17179/excli2021-3790 .

Ajdžanović, Vladimir, Filipović, Branko, Šošić-Jurjević, Branka , Miler, Marko, Milošević, Verica, "Margins of beneficial daily dosage of supplements in prevention of COVID-19" in EXCLI Journal, 20 (2021):828-834,
https://doi.org/10.17179/excli2021-3790 . .

TY  - JOUR
AU  - Miler, Marko
AU  - Ajdžanović, Vladimir
AU  - Živanović, Jasmina
AU  - Marković Filipović, Jelena
AU  - Šošić-Jurjević, Branka 
AU  - Milošević, Verica
PY  - 2021
UR  - https://www.cambridge.org/core/journals/microscopy-and-microanalysis/article/abs/thyroid-gland-alterations-in-oldaged-wistar-rats-a-comprehensive-stereological-ultrastructural-hormonal-and-gene-expression-study/4C35B9A6AFED404C65272C08C8C2DC27
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4143
AB  - The aim of the present study was to determine and elaborate on all changes in old-aged (OA) versus young-aged (YA) rat thyroids by using
stereological, ultrastructural, hormonal, and gene expression analyses. We used 4- and 24-month-old male Wistar rats in our evaluation,
presenting all changes in comparison with YA rats. Results showed that the thyroid parenchyma was characterized by higher absolute
volumes of the gland, colloid, epithelium, and interstitium by 135, 135, 140, and 142% ( p < 0.05) respectively, while the relative volumes
of colloid and glands were unchanged. Ultrastructural analysis revealed less active glands, with smaller amounts of lysosomes, thyroglobulin
(Tg) granules, and microvilli in the luminal colloid. Optical density values for thyroid peroxidase (TPO), Tg, and vascular-endothelial
growth factor immunostaining remained unchanged; however, TPO and Tg exhibited visually stronger expression in small active follicles.
Thyroxine (T4)-Tg, the relative intensity of fluorescence (RIF), serum T4, and the sodium-iodide symporter immunohistochemical and gene
expressions decreased by 20, 40, 29, and 31% ( p < 0.05), respectively, in OA thyroids. Pituitary thyroid-stimulating hormone (TSH) RIF
increased by 44% ( p < 0.05), but the TSH serum concentration remained unchanged. In conclusion, the obtained results indicate depression
of the thyroid gland synthetic and secretory capacity with advanced age.
PB  - Cambridge University Press
T2  - Microscopy and Microanalysis
T1  - Thyroid Gland Alterations in Old-Aged Wistar Rats: A Comprehensive Stereological, Ultrastructural, Hormonal, and Gene Expression Study
DO  - 10.1017/S1431927621000064
ER  - 

@article{
author = "Miler, Marko and Ajdžanović, Vladimir and Živanović, Jasmina and Marković Filipović, Jelena and Šošić-Jurjević, Branka  and Milošević, Verica",
year = "2021",
abstract = "The aim of the present study was to determine and elaborate on all changes in old-aged (OA) versus young-aged (YA) rat thyroids by using
stereological, ultrastructural, hormonal, and gene expression analyses. We used 4- and 24-month-old male Wistar rats in our evaluation,
presenting all changes in comparison with YA rats. Results showed that the thyroid parenchyma was characterized by higher absolute
volumes of the gland, colloid, epithelium, and interstitium by 135, 135, 140, and 142% ( p < 0.05) respectively, while the relative volumes
of colloid and glands were unchanged. Ultrastructural analysis revealed less active glands, with smaller amounts of lysosomes, thyroglobulin
(Tg) granules, and microvilli in the luminal colloid. Optical density values for thyroid peroxidase (TPO), Tg, and vascular-endothelial
growth factor immunostaining remained unchanged; however, TPO and Tg exhibited visually stronger expression in small active follicles.
Thyroxine (T4)-Tg, the relative intensity of fluorescence (RIF), serum T4, and the sodium-iodide symporter immunohistochemical and gene
expressions decreased by 20, 40, 29, and 31% ( p < 0.05), respectively, in OA thyroids. Pituitary thyroid-stimulating hormone (TSH) RIF
increased by 44% ( p < 0.05), but the TSH serum concentration remained unchanged. In conclusion, the obtained results indicate depression
of the thyroid gland synthetic and secretory capacity with advanced age.",
publisher = "Cambridge University Press",
journal = "Microscopy and Microanalysis",
title = "Thyroid Gland Alterations in Old-Aged Wistar Rats: A Comprehensive Stereological, Ultrastructural, Hormonal, and Gene Expression Study",
doi = "10.1017/S1431927621000064"
}

Miler, M., Ajdžanović, V., Živanović, J., Marković Filipović, J., Šošić-Jurjević, B.,& Milošević, V.. (2021). Thyroid Gland Alterations in Old-Aged Wistar Rats: A Comprehensive Stereological, Ultrastructural, Hormonal, and Gene Expression Study. in Microscopy and Microanalysis
Cambridge University Press..
https://doi.org/10.1017/S1431927621000064

Miler M, Ajdžanović V, Živanović J, Marković Filipović J, Šošić-Jurjević B, Milošević V. Thyroid Gland Alterations in Old-Aged Wistar Rats: A Comprehensive Stereological, Ultrastructural, Hormonal, and Gene Expression Study. in Microscopy and Microanalysis. 2021;.
doi:10.1017/S1431927621000064 .

Miler, Marko, Ajdžanović, Vladimir, Živanović, Jasmina, Marković Filipović, Jelena, Šošić-Jurjević, Branka , Milošević, Verica, "Thyroid Gland Alterations in Old-Aged Wistar Rats: A Comprehensive Stereological, Ultrastructural, Hormonal, and Gene Expression Study" in Microscopy and Microanalysis (2021),
https://doi.org/10.1017/S1431927621000064 . .

TY  - JOUR
AU  - Vodnik, Vesna V.
AU  - Mojić, Marija
AU  - Stamenović, Una
AU  - Otoničar, Mojca
AU  - Ajdžanović, Vladimir
AU  - Maksimović-Ivanić, Danijela
AU  - Mijatović, Sanja
AU  - Marković, Mirjana M.
AU  - Barudžija, Tanja
AU  - Filipović, Branko
AU  - Milošević, Verica
AU  - Šošić-Jurjević, Branka 
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4238
AB  - Soy isoflavone genistein (Gen) exerts beneficial effects against prostate cancer cells in vitro and in vivo. However, its use as a chemoprevention/therapeutic agent is largely limited due to its low bioavailability. In this study we synthesized two variants of a new delivery system, genistein–gold nanoparticles conjugates Gen@AuNPs1 and Gen@AuNPs2, by an environmentally friendly method, using a dual role of Gen to reduce Au3+ and stabilize the formed AuNPs, with no additional component. The formation of Gen@AuNPs was confirmed via UV–Vis spectroscopy, FTIR, and Raman spectra measurements. The spherical shape and uniform size of Gen@AuNPs1 and Gen@AuNPs2 (10 ± 2 and 23 ± 3 nm, respectively), were determined by transmission electron microscopy. The nano-conjugates also varied in hydrodynamic diameter (65.0 ± 1.7 and 153.0 ± 2.2 nm) but had similar negative zeta potential (−35.0 ± 2.5 and −37.0 ± 1.6 mV), as measured by dynamic light scattering. The Gen loading was estimated to be 46 and 48%, for Gen@AuNPs1 and Gen@AuNPs2, respectively. The antiproliferative activities of GenAuNPs were confirmed by MTT test in vitro on three malignant prostate carcinoma cell lines (PC3, DU 145, and LNCaP), while selectivity toward malignant phenotype was confirmed using non-cancerous MRC-5 cells. Flow cytometric analysis showed that the inhibition on cell proliferation of more potent Gen@AuNPs1 nano-conjugate is comparable with the effects of free Gen. In conclusion, the obtained results, including physicochemical characterization of newly synthesized AuNPs loaded with Gen, cytotoxicity, and IC50 assessments, indicate their stability and bioactivity as an antioxidant and anti-prostate cancer agent, with low toxicity against human primary cells.
PB  - Elsevier Ltd
T2  - Materials Science and Engineering C
T1  - Development of genistein-loaded gold nanoparticles and their antitumor potential against prostate cancer cell lines
VL  - 124
DO  - 10.1016/j.msec.2021.112078
SP  - 112078
ER  - 

@article{
author = "Vodnik, Vesna V. and Mojić, Marija and Stamenović, Una and Otoničar, Mojca and Ajdžanović, Vladimir and Maksimović-Ivanić, Danijela and Mijatović, Sanja and Marković, Mirjana M. and Barudžija, Tanja and Filipović, Branko and Milošević, Verica and Šošić-Jurjević, Branka ",
year = "2021",
abstract = "Soy isoflavone genistein (Gen) exerts beneficial effects against prostate cancer cells in vitro and in vivo. However, its use as a chemoprevention/therapeutic agent is largely limited due to its low bioavailability. In this study we synthesized two variants of a new delivery system, genistein–gold nanoparticles conjugates Gen@AuNPs1 and Gen@AuNPs2, by an environmentally friendly method, using a dual role of Gen to reduce Au3+ and stabilize the formed AuNPs, with no additional component. The formation of Gen@AuNPs was confirmed via UV–Vis spectroscopy, FTIR, and Raman spectra measurements. The spherical shape and uniform size of Gen@AuNPs1 and Gen@AuNPs2 (10 ± 2 and 23 ± 3 nm, respectively), were determined by transmission electron microscopy. The nano-conjugates also varied in hydrodynamic diameter (65.0 ± 1.7 and 153.0 ± 2.2 nm) but had similar negative zeta potential (−35.0 ± 2.5 and −37.0 ± 1.6 mV), as measured by dynamic light scattering. The Gen loading was estimated to be 46 and 48%, for Gen@AuNPs1 and Gen@AuNPs2, respectively. The antiproliferative activities of GenAuNPs were confirmed by MTT test in vitro on three malignant prostate carcinoma cell lines (PC3, DU 145, and LNCaP), while selectivity toward malignant phenotype was confirmed using non-cancerous MRC-5 cells. Flow cytometric analysis showed that the inhibition on cell proliferation of more potent Gen@AuNPs1 nano-conjugate is comparable with the effects of free Gen. In conclusion, the obtained results, including physicochemical characterization of newly synthesized AuNPs loaded with Gen, cytotoxicity, and IC50 assessments, indicate their stability and bioactivity as an antioxidant and anti-prostate cancer agent, with low toxicity against human primary cells.",
publisher = "Elsevier Ltd",
journal = "Materials Science and Engineering C",
title = "Development of genistein-loaded gold nanoparticles and their antitumor potential against prostate cancer cell lines",
volume = "124",
doi = "10.1016/j.msec.2021.112078",
pages = "112078"
}

Vodnik, V. V., Mojić, M., Stamenović, U., Otoničar, M., Ajdžanović, V., Maksimović-Ivanić, D., Mijatović, S., Marković, M. M., Barudžija, T., Filipović, B., Milošević, V.,& Šošić-Jurjević, B.. (2021). Development of genistein-loaded gold nanoparticles and their antitumor potential against prostate cancer cell lines. in Materials Science and Engineering C
Elsevier Ltd., 124, 112078.
https://doi.org/10.1016/j.msec.2021.112078

Vodnik VV, Mojić M, Stamenović U, Otoničar M, Ajdžanović V, Maksimović-Ivanić D, Mijatović S, Marković MM, Barudžija T, Filipović B, Milošević V, Šošić-Jurjević B. Development of genistein-loaded gold nanoparticles and their antitumor potential against prostate cancer cell lines. in Materials Science and Engineering C. 2021;124:112078.
doi:10.1016/j.msec.2021.112078 .

Vodnik, Vesna V., Mojić, Marija, Stamenović, Una, Otoničar, Mojca, Ajdžanović, Vladimir, Maksimović-Ivanić, Danijela, Mijatović, Sanja, Marković, Mirjana M., Barudžija, Tanja, Filipović, Branko, Milošević, Verica, Šošić-Jurjević, Branka , "Development of genistein-loaded gold nanoparticles and their antitumor potential against prostate cancer cell lines" in Materials Science and Engineering C, 124 (2021):112078,
https://doi.org/10.1016/j.msec.2021.112078 . .

TY  - JOUR
AU  - Rauš Balind, Snežana
AU  - Manojlović-Stojanoski, Milica
AU  - Šošić-Jurjević, Branka 
AU  - Selaković, Vesna
AU  - Milošević, Verica
AU  - Petković, Branka
PY  - 2020
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1002/bem.22237
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3570
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3764
AB  - The neuroendocrine system can be modulated by a magnetic field and cerebral ischemia as external and internal stressors, respectively. This study deals with the separate or combined effects of an extremely low frequency (ELF) magnetic field (50 Hz, average magnetic field of 0.5 mT) for 7 days and global cerebral ischemia for 10 min on the morpho-functional features of pituitary adrenocorticotrophic (ACTH) and thyrotrophic (TSH) cells in 3-month-old gerbils. To determine the immediate and delayed effects of the applied stressors, measurements were made on the 7th and 14th days after the onset of the experiment. The ELF magnetic field and 10-min global cerebral ischemia, separately and particularly in combination, decreased (P < 0.05) the volume density of ACTH cells, while only in combination were intracellular ACTH content and plasma ACTH concentration increased (P < 0.05) on day 7. The ELF magnetic field elevated serum TSH concentration on day 7 and intracellular TSHβ content on day 14 (P < 0.05). Also, 10-min global cerebral ischemia alone increased serum TSH concentration (P < 0.05), while in combination with the ELF magnetic field it elevated (P < 0.05) intracellular TSHβ content on day 14. In conclusion, an ELF magnetic field and/or 10-min global cerebral ischemia can induce immediate and delayed stimulation of ACTH and TSH synthesis and secretion.
PB  - John Wiley & Sons, Ltd
T2  - Bioelectromagnetics
T1  - An Extremely Low Frequency Magnetic Field and Global Cerebral Ischemia Affect Pituitary ACTH and TSH Cells in Gerbils
IS  - 2
VL  - 41
DO  - 10.1002/bem.22237
SP  - 91
EP  - 103
ER  - 

@article{
author = "Rauš Balind, Snežana and Manojlović-Stojanoski, Milica and Šošić-Jurjević, Branka  and Selaković, Vesna and Milošević, Verica and Petković, Branka",
year = "2020",
abstract = "The neuroendocrine system can be modulated by a magnetic field and cerebral ischemia as external and internal stressors, respectively. This study deals with the separate or combined effects of an extremely low frequency (ELF) magnetic field (50 Hz, average magnetic field of 0.5 mT) for 7 days and global cerebral ischemia for 10 min on the morpho-functional features of pituitary adrenocorticotrophic (ACTH) and thyrotrophic (TSH) cells in 3-month-old gerbils. To determine the immediate and delayed effects of the applied stressors, measurements were made on the 7th and 14th days after the onset of the experiment. The ELF magnetic field and 10-min global cerebral ischemia, separately and particularly in combination, decreased (P < 0.05) the volume density of ACTH cells, while only in combination were intracellular ACTH content and plasma ACTH concentration increased (P < 0.05) on day 7. The ELF magnetic field elevated serum TSH concentration on day 7 and intracellular TSHβ content on day 14 (P < 0.05). Also, 10-min global cerebral ischemia alone increased serum TSH concentration (P < 0.05), while in combination with the ELF magnetic field it elevated (P < 0.05) intracellular TSHβ content on day 14. In conclusion, an ELF magnetic field and/or 10-min global cerebral ischemia can induce immediate and delayed stimulation of ACTH and TSH synthesis and secretion.",
publisher = "John Wiley & Sons, Ltd",
journal = "Bioelectromagnetics",
title = "An Extremely Low Frequency Magnetic Field and Global Cerebral Ischemia Affect Pituitary ACTH and TSH Cells in Gerbils",
number = "2",
volume = "41",
doi = "10.1002/bem.22237",
pages = "91-103"
}

Rauš Balind, S., Manojlović-Stojanoski, M., Šošić-Jurjević, B., Selaković, V., Milošević, V.,& Petković, B.. (2020). An Extremely Low Frequency Magnetic Field and Global Cerebral Ischemia Affect Pituitary ACTH and TSH Cells in Gerbils. in Bioelectromagnetics
John Wiley & Sons, Ltd., 41(2), 91-103.
https://doi.org/10.1002/bem.22237

Rauš Balind S, Manojlović-Stojanoski M, Šošić-Jurjević B, Selaković V, Milošević V, Petković B. An Extremely Low Frequency Magnetic Field and Global Cerebral Ischemia Affect Pituitary ACTH and TSH Cells in Gerbils. in Bioelectromagnetics. 2020;41(2):91-103.
doi:10.1002/bem.22237 .

Rauš Balind, Snežana, Manojlović-Stojanoski, Milica, Šošić-Jurjević, Branka , Selaković, Vesna, Milošević, Verica, Petković, Branka, "An Extremely Low Frequency Magnetic Field and Global Cerebral Ischemia Affect Pituitary ACTH and TSH Cells in Gerbils" in Bioelectromagnetics, 41, no. 2 (2020):91-103,
https://doi.org/10.1002/bem.22237 . .

TY  - JOUR
AU  - Ajdžanović, Vladimir
AU  - Miler, Marko
AU  - Živanović, Jasmina
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka 
AU  - Popovska-Perčinić, Florina
AU  - Milošević, Verica
PY  - 2020
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32120001
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3627
AB  - INTRODUCTION AND AIM Daidzein application may represent an effective and less harmful alternative to indicated, classical estrogenization of ageing men. The aim of this study was to perform structural and hormonal analysis of the adrenal cortex, after estradiol or daidzein supplementation in a rat model of the andropause. MATERIAL AND METHODS Middle-aged Wistar rats were divided into sham operated (SO; n = 8), orchidectomized (Orx; n = 8), estradiol treated orchidectomized (Orx + E; n = 8) and daidzein treated orchidectomized (Orx + D; n = 8) groups. Estradiol (0.625 mg/kg b.m./day) or daidzein (30 mg/kg b.m./day) were administered subcutaneously for three weeks, while the SO and Orx groups received the vehicle alone. Set objectives were achieved using stereology, histochemistry/immunohistochemistry, immunoassays and ultrastructural analysis. RESULTS Both estradiol and daidzein treatment significantly increased volumes of the zona glomerulosa cell and nuclei, but decreased circulating aldosterone levels. Estradiol markedly increased volumes of the zona fasciculata cell and nuclei in parallel with significant decrease of the adrenal tissue level of corticosterone, while daidzein significantly decreased both the adrenal and circulating levels of corticosterone. Serum DHEA level and volumes of the zona reticularis cell and nuclei significantly increased upon estradiol treatment, whereas daidzein even stronger increased the circulating level of DHEA. Shunting of the corticosteroidogenesis pathways towards adrenal androgens production, after the treatments, corresponded to the ultrastructural findings and zonal capillary network rearrangements. CONCLUSIONS Given the coherence of its effects and relative safety, daidzein could be the remedy of choice for the treatment of ageing-caused androgen deprivation and the hypothalamo-pituitary-adrenal axis hyperfunction/related metabolic issues in males.
T2  - Annals of Anatomy
T1  - The adrenal cortex after estradiol or daidzein application in a rat model of the andropause: Structural and hormonal study.
VL  - 230
DO  - 10.1016/j.aanat.2020.151487
SP  - 151487
ER  - 

@article{
author = "Ajdžanović, Vladimir and Miler, Marko and Živanović, Jasmina and Filipović, Branko and Šošić-Jurjević, Branka  and Popovska-Perčinić, Florina and Milošević, Verica",
year = "2020",
abstract = "INTRODUCTION AND AIM Daidzein application may represent an effective and less harmful alternative to indicated, classical estrogenization of ageing men. The aim of this study was to perform structural and hormonal analysis of the adrenal cortex, after estradiol or daidzein supplementation in a rat model of the andropause. MATERIAL AND METHODS Middle-aged Wistar rats were divided into sham operated (SO; n = 8), orchidectomized (Orx; n = 8), estradiol treated orchidectomized (Orx + E; n = 8) and daidzein treated orchidectomized (Orx + D; n = 8) groups. Estradiol (0.625 mg/kg b.m./day) or daidzein (30 mg/kg b.m./day) were administered subcutaneously for three weeks, while the SO and Orx groups received the vehicle alone. Set objectives were achieved using stereology, histochemistry/immunohistochemistry, immunoassays and ultrastructural analysis. RESULTS Both estradiol and daidzein treatment significantly increased volumes of the zona glomerulosa cell and nuclei, but decreased circulating aldosterone levels. Estradiol markedly increased volumes of the zona fasciculata cell and nuclei in parallel with significant decrease of the adrenal tissue level of corticosterone, while daidzein significantly decreased both the adrenal and circulating levels of corticosterone. Serum DHEA level and volumes of the zona reticularis cell and nuclei significantly increased upon estradiol treatment, whereas daidzein even stronger increased the circulating level of DHEA. Shunting of the corticosteroidogenesis pathways towards adrenal androgens production, after the treatments, corresponded to the ultrastructural findings and zonal capillary network rearrangements. CONCLUSIONS Given the coherence of its effects and relative safety, daidzein could be the remedy of choice for the treatment of ageing-caused androgen deprivation and the hypothalamo-pituitary-adrenal axis hyperfunction/related metabolic issues in males.",
journal = "Annals of Anatomy",
title = "The adrenal cortex after estradiol or daidzein application in a rat model of the andropause: Structural and hormonal study.",
volume = "230",
doi = "10.1016/j.aanat.2020.151487",
pages = "151487"
}

Ajdžanović, V., Miler, M., Živanović, J., Filipović, B., Šošić-Jurjević, B., Popovska-Perčinić, F.,& Milošević, V.. (2020). The adrenal cortex after estradiol or daidzein application in a rat model of the andropause: Structural and hormonal study.. in Annals of Anatomy, 230, 151487.
https://doi.org/10.1016/j.aanat.2020.151487

Ajdžanović V, Miler M, Živanović J, Filipović B, Šošić-Jurjević B, Popovska-Perčinić F, Milošević V. The adrenal cortex after estradiol or daidzein application in a rat model of the andropause: Structural and hormonal study.. in Annals of Anatomy. 2020;230:151487.
doi:10.1016/j.aanat.2020.151487 .

Ajdžanović, Vladimir, Miler, Marko, Živanović, Jasmina, Filipović, Branko, Šošić-Jurjević, Branka , Popovska-Perčinić, Florina, Milošević, Verica, "The adrenal cortex after estradiol or daidzein application in a rat model of the andropause: Structural and hormonal study." in Annals of Anatomy, 230 (2020):151487,
https://doi.org/10.1016/j.aanat.2020.151487 . .